CN114391446A - Production method of detoxified raw ginger seeds - Google Patents
Production method of detoxified raw ginger seeds Download PDFInfo
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- CN114391446A CN114391446A CN202210081239.0A CN202210081239A CN114391446A CN 114391446 A CN114391446 A CN 114391446A CN 202210081239 A CN202210081239 A CN 202210081239A CN 114391446 A CN114391446 A CN 114391446A
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- 235000006886 Zingiber officinale Nutrition 0.000 title claims abstract description 69
- 235000008397 ginger Nutrition 0.000 title claims abstract description 69
- 241000234314 Zingiber Species 0.000 title claims abstract description 68
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 19
- 239000004576 sand Substances 0.000 claims abstract description 21
- BSYNRYMUTXBXSQ-UHFFFAOYSA-N Aspirin Chemical compound CC(=O)OC1=CC=CC=C1C(O)=O BSYNRYMUTXBXSQ-UHFFFAOYSA-N 0.000 claims abstract description 20
- 229960001138 acetylsalicylic acid Drugs 0.000 claims abstract description 20
- 239000001963 growth medium Substances 0.000 claims abstract description 15
- 239000011159 matrix material Substances 0.000 claims abstract description 14
- LJXTYJXBORAIHX-UHFFFAOYSA-N diethyl 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate Chemical compound CCOC(=O)C1=C(C)NC(C)=C(C(=O)OCC)C1 LJXTYJXBORAIHX-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000007864 aqueous solution Substances 0.000 claims abstract description 12
- 238000012258 culturing Methods 0.000 claims abstract description 11
- 238000005507 spraying Methods 0.000 claims abstract description 11
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000004202 carbamide Substances 0.000 claims abstract description 6
- 201000010099 disease Diseases 0.000 claims abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 239000002689 soil Substances 0.000 claims description 17
- 229910052902 vermiculite Inorganic materials 0.000 claims description 17
- 235000019354 vermiculite Nutrition 0.000 claims description 17
- 239000010455 vermiculite Substances 0.000 claims description 17
- 239000003337 fertilizer Substances 0.000 claims description 11
- 230000002363 herbicidal effect Effects 0.000 claims description 11
- 239000004009 herbicide Substances 0.000 claims description 11
- 235000015097 nutrients Nutrition 0.000 claims description 11
- 239000000243 solution Substances 0.000 claims description 8
- 238000003306 harvesting Methods 0.000 claims description 6
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 5
- 241000196324 Embryophyta Species 0.000 claims description 5
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 5
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 5
- 239000006013 carbendazim Substances 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 5
- 229910052700 potassium Inorganic materials 0.000 claims description 5
- 239000011591 potassium Substances 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims description 3
- 229920001817 Agar Polymers 0.000 claims description 3
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 claims description 3
- 229960001669 kinetin Drugs 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 238000009826 distribution Methods 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- 230000003385 bacteriostatic effect Effects 0.000 abstract description 4
- 241000724252 Cucumber mosaic virus Species 0.000 abstract description 3
- 241000723873 Tobacco mosaic virus Species 0.000 abstract description 3
- 239000000758 substrate Substances 0.000 description 25
- 230000000052 comparative effect Effects 0.000 description 11
- 238000001784 detoxification Methods 0.000 description 4
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 241000700605 Viruses Species 0.000 description 1
- 244000273928 Zingiber officinale Species 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000001841 zingiber officinale Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Soil Sciences (AREA)
- Forests & Forestry (AREA)
- Ecology (AREA)
- Botany (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention relates to the technical field of detoxified ginger production, in particular to a production method of a detoxified ginger stock seed, which comprises the following steps: s1, burying disease-free ginger blocks in wet fine sand, culturing at room temperature, taking 1cm stem tips when the buds grow to 1-2cm, sterilizing, stripping the stem tips with 1-2 leaf primordia, and inoculating to a prepared culture medium; s2, placing the culture medium in an insect-proof net room for culture; s3, when the sprouts at the stem tips grow to 3-5 cm in height and have 4-5 leaves, planting the sprouts in a matrix for cultivation, spraying an aspirin aqueous solution after the planting is finished, and covering a mulching film, wherein the matrix is mixed with 3, 5-dicarbethoxy-2, 6-dimethyl-1, 4-dihydropyridine; s4, shading by using a shading net when the ginger seedlings emerge 20 days after cultivation, additionally fertilizing 25-30 kg of urea, and removing the shading net before and after the beginning of autumn. The invention not only can effectively promote the ginger to root, but also can improve the bacteriostatic effect of the ginger on tobacco mosaic virus and cucumber mosaic virus.
Description
Technical Field
The invention relates to the technical field of detoxified ginger production, in particular to a production method of a detoxified ginger original seed.
Background
The detoxified ginger is produced by combining heat treatment and stem tip culture to obtain test-tube seedling, planting in insect-proof net room, strengthening soil, fertilizer and water management, harvesting in autumn to obtain original seed, planting in the next year to obtain original seed, and planting in the first year to obtain production seed. After the ginger is detoxified, the excellent properties of the ginger are recovered, detoxified cultivation is realized, the quality of the ginger can be improved, the morbidity is reduced, and the commodity and the economic benefit of the ginger are improved.
Therefore, we propose a method for producing detoxified ginger stock seeds to solve the above problems.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a production method of detoxified ginger original seeds.
A production method of detoxified ginger original seeds comprises the following steps:
s1, burying disease-free ginger blocks in wet fine sand, culturing at room temperature, taking 1cm stem tips when the buds grow to 1-2cm, sterilizing, stripping the stem tips with 1-2 leaf primordia, and inoculating to a prepared culture medium;
s2, placing the culture medium in an insect-proof net room for culture;
s3, when the sprouts at the stem tips grow to 3-5 cm in height and have 4-5 leaves, planting the sprouts in a matrix for cultivation, spraying an aspirin aqueous solution after the planting is finished, and covering a mulching film, wherein the matrix is mixed with 3, 5-dicarbethoxy-2, 6-dimethyl-1, 4-dihydropyridine;
s4, shading by using a shading net when ginger seedlings emerge 20 days after cultivation, additionally fertilizing 25-30 kg of urea, removing the shading net before and after 'early autumn', removing a mulching film for additionally fertilizing 110 kg of compound fertilizer and 50-60 kg of potassium fertilizer in a ditch 15-17 cm away from the plant base, then covering soil, sealing the ditch and ridging, and finally watering thoroughly;
s5, erecting an arch shed above the ginger seedlings before blooming to prolong the growth of the ginger for 20-30 days, and harvesting to obtain the virus-free ginger stock seeds.
Preferably, the treatment process of the wet fine sand in S1 is as follows: soaking the fine sand in 800 times of 50% carbendazim solution.
Preferably, the culture medium in the S1 is MS + 3% of sucrose + 0.5% of agar +1mg/L of KT kinetin +1mg/L of naphthylacetic acid, and the pH value is adjusted to 5.8.
Preferably, the conditions for the culture in step S2 are: culturing under the conditions of 12 hours/day of illumination, 4000 lux of light intensity and 26 +/-2 ℃.
Preferably, the distribution of the matrix in S3 is: the lower layer is nutrient soil, the middle layer is river sand and the upper layer is vermiculite.
Preferably, before the vermiculite is used, the water is uniformly poured to ensure that the water holding capacity of the vermiculite reaches a saturated state.
Preferably, in S3, before the mulching film is coated, the mulching film is sprayed with the herbicide mixed with water.
Preferably, the ratio of aspirin to water in the aspirin aqueous solution in S3 is 1:1000, and the aspirin is ground powder.
Preferably, the amount of the 3, 5-dicarbethoxy-2, 6-dimethyl-1, 4-dihydropyridine in the S3 is 3-5% of the mass of the matrix.
Preferably, in the step S4, after the sunshade net is removed, water is poured for 1 time every 7 to 8 days.
Compared with the prior art, the invention has the beneficial effects that:
1. after the stem tip is planted in the matrix, the water solution of aspirin is sprayed to the matrix, so that the rooting of the ginger can be promoted more effectively.
2. A small amount of 3, 5-dicarbethoxy-2, 6-dimethyl-1, 4-dihydropyridine is mixed in the substrate planted in the stem tip, so that the antibacterial effect of the ginger can be improved.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples.
Example 1:
a production method of detoxified ginger original seeds comprises the following steps:
s1, burying disease-free ginger blocks in fine sand soaked in 800 times of 50% carbendazim solution, culturing at room temperature, taking 1cm of stem tips after the buds grow to 1-2cm, sterilizing, stripping the stem tips with 1-2 leaf primordia, and inoculating to a prepared culture medium;
s2, placing the culture medium in an insect-proof net room, and culturing under the conditions of illumination for 12 hours/day, light intensity of 4000 luxes and temperature of 24 ℃;
s3, when the shoot tip sprouts to 3-5 cm high and has 4-5 leaves, planting the shoot tip in a substrate with a lower layer of nutrient soil, a middle layer of river sand and an upper layer of vermiculite, spraying an aspirin aqueous solution after planting, covering with a mulching film, spraying a herbicide with water before covering with the mulching film, and mixing 3, 5-dicarbethoxy-2, 6-dimethyl-1, 4-dihydropyridine with the mass of the substrate in the substrate;
s4, shading by using a sunshade net when ginger seedlings emerge 20 days after cultivation, additionally fertilizing 25 kg of urea, removing the sunshade net before and after "beginning of autumn", removing a mulching film for additionally fertilizing 100 kg of compound fertilizer and 50 kg of potassium fertilizer in a ditch 15 cm away from the plant base, then covering soil, sealing the ditch, ridging, finally watering thoroughly, and watering 1 time every 7 days after the sunshade net is removed;
s5, erecting an arch shed above the ginger seedlings before blooming to prolong the growth of the ginger for 20-30 days, and harvesting to obtain the virus-free ginger stock seeds.
Example 2:
s1, burying disease-free ginger blocks in fine sand soaked in 800 times of 50% carbendazim solution, culturing at room temperature, taking 1cm of stem tips after the buds grow to 1-2cm, sterilizing, stripping the stem tips with 1-2 leaf primordia, and inoculating to a prepared culture medium;
s2, placing the culture medium in an insect-proof net room, and culturing under the conditions of illumination for 12 hours/day, light intensity of 4000 luxes and temperature of 26 ℃;
s3, when the shoot tip sprouts to 3-5 cm high and has 4-5 leaves, planting the shoot tip in a substrate with a lower layer of nutrient soil, a middle layer of river sand and an upper layer of vermiculite, spraying an aspirin aqueous solution after planting, covering with a mulching film, spraying a herbicide with water before covering with the mulching film, and mixing 3, 5-dicarbethoxy-2, 6-dimethyl-1, 4-dihydropyridine with the mass of the substrate, wherein the mass of the substrate is 4%;
s4, shading by using a sunshade net when ginger seedlings emerge 20 days after cultivation, additionally fertilizing 27 kg of urea, removing the sunshade net before and after the beginning of autumn, removing a mulching film, additionally fertilizing 105 kg of compound fertilizer and 55 kg of potassium fertilizer in a ditch 15 cm away from the plant base, then covering soil, sealing the ditch, ridging, finally watering thoroughly, and watering 1 time every 7 days after the sunshade net is removed;
s5, erecting an arch shed above the ginger seedlings before blooming to prolong the growth of the ginger for 20-30 days, and harvesting to obtain the virus-free ginger stock seeds.
Example 3:
s1, burying disease-free ginger blocks in fine sand soaked in 800 times of 50% carbendazim solution, culturing at room temperature, taking 1cm of stem tips after the buds grow to 1-2cm, sterilizing, stripping the stem tips with 1-2 leaf primordia, and inoculating to a prepared culture medium;
s2, placing the culture medium in an insect-proof net room, and culturing under the conditions of illumination for 12 hours/day, light intensity of 4000 luxes and temperature of 28 ℃;
s3, when the shoot tip sprouts to 3-5 cm high and has 4-5 leaves, planting the shoot tip in a substrate with a lower layer of nutrient soil, a middle layer of river sand and an upper layer of vermiculite, spraying an aspirin aqueous solution after planting, covering with a mulching film, spraying a herbicide with water before covering with the mulching film, and mixing 3, 5-dicarbethoxy-2, 6-dimethyl-1, 4-dihydropyridine with the mass of 5% of the substrate in the substrate;
s4, shading by using a sunshade net when ginger seedlings emerge 20 days after cultivation, additionally fertilizing 30 kilograms of urea, removing the sunshade net before and after the beginning of autumn, removing a mulching film for additionally fertilizing 110 kilograms of compound fertilizer and 60 kilograms of potassium fertilizer, additionally fertilizing in a ditch 15 centimeters away from the plant base, then covering soil, sealing the ditch, ridging, finally watering thoroughly, and watering 1 time every 7 days after the sunshade net is removed;
s5, erecting an arch shed above the ginger seedlings before blooming to prolong the growth of the ginger for 20-30 days, and harvesting to obtain the virus-free ginger stock seeds.
Wherein the culture medium used in the above examples 1-3 was MS + 3% sucrose + 0.5% agar +1mg/L KT kinetin +1mg/L naphthylacetic acid, and the pH was adjusted to 5.8.
In the used matrix, vermiculite is uniformly poured by water before use, so that the water holding capacity of the vermiculite reaches a saturated state; the ratio of aspirin to water in the aspirin water solution is 1:1000, and the aspirin is ground powder.
Test one: determination of rooting rate of detoxified ginger original seed
Comparative example 1: compared with the example 1, except that the step S3 is changed into 'when the stem tip sprouts to 3-5 cm high and has 4-5 leaves, the stem tip sprouts are planted in a substrate with the lower layer being nutrient soil, the middle layer being river sand and the upper layer being vermiculite to carry out cultivation, after the cultivation is finished, a herbicide is added with water to be sprayed, a mulching film is covered, and 3, 5-dicarbethoxy-2, 6-dimethyl-1, 4-dihydropyridine accounting for 3 percent of the mass of the substrate is mixed in the substrate', the other steps are consistent;
comparative example 2: compared with the example 2, except that the step S3 is changed into 'when the stem tip sprouts to 3-5 cm high and has 4-5 leaves, the stem tip sprouts are planted in a substrate with the lower layer being nutrient soil, the middle layer being river sand and the upper layer being vermiculite to carry out cultivation, after the cultivation is finished, a herbicide is added with water to be sprayed, a mulching film is covered, and 3, 5-dicarbethoxy-2, 6-dimethyl-1, 4-dihydropyridine accounting for 3 percent of the mass of the substrate is mixed in the substrate', the other steps are consistent;
comparative example 3: compared with the example 3, except that the step S3 is changed into 'when the stem tip sprouts to 3-5 cm high and has 4-5 leaves, the stem tip sprouts are planted in a substrate with the lower layer being nutrient soil, the middle layer being river sand and the upper layer being vermiculite to carry out cultivation, after the cultivation is finished, the herbicide is added with water to be sprayed, a mulching film is covered, and 3, 5-dicarbethoxy-2, 6-dimethyl-1, 4-dihydropyridine accounting for 3 percent of the mass of the substrate is mixed in the substrate', the other steps are consistent;
the detoxified ginger stock seeds of examples 1-3 and comparative examples 1-3 were tested and the rooting of the ginger stock seeds was periodically observed and recorded in the following table:
from the above test results, it can be seen that the rooting rate of the detoxified ginger stock seeds in examples 1-3 continuously increases with the time, which may approach 50%, the rooting rate of the detoxified ginger stock seeds in comparative examples 1-3 increases slowly, and after a period of time, the rooting rate begins to approach a steady state, and thus, spraying the aqueous solution of aspirin into the matrix can effectively promote the rooting of ginger.
And (2) test II: determination of bacteriostatic effect on detoxified ginger original seed
Comparative example 4: compared with the embodiment 1, the steps are the same except that the step S3 is changed into the steps that when the stem tip sprouts to 3-5 cm high and has 4-5 leaves, the stem tip sprouts are planted in a substrate with the lower layer being nutrient soil, the middle layer being river sand and the upper layer being vermiculite to be cultivated, after the planting is finished, an aspirin aqueous solution is sprayed, a mulching film is covered, and a herbicide is mixed with water and sprayed before the mulching film is covered;
comparative example 5: compared with the embodiment 2, the steps are the same except that the step S3 is changed into the steps that when the stem tip sprouts to 3-5 cm high and has 4-5 leaves, the stem tip sprouts are planted in a substrate with the lower layer being nutrient soil, the middle layer being river sand and the upper layer being vermiculite, the substrate is planted, the aspirin aqueous solution is sprayed after the stem tip sprouts are planted, the mulching film is covered, and the herbicide is mixed with water and sprayed before the mulching film is covered;
comparative example 6: compared with the embodiment 3, the steps are the same except that the step S3 is changed into the steps that when the stem tip sprouts to 3-5 cm high and has 4-5 leaves, the stem tip sprouts are planted in a substrate with the lower layer being nutrient soil, the middle layer being river sand and the upper layer being vermiculite, the substrate is planted, the aspirin aqueous solution is sprayed after the planting is finished, the mulching film is covered, and the herbicide is mixed with water and sprayed before the mulching film is covered;
the original seeds of detoxified ginger of the above examples 1-3 and comparative examples 4-6 were subjected to bacteriostatic tests according to the contents of standard No. NY/T404-2000 technical Specification for detecting virus of detoxified ginger seeds (Zingiber officinale) and the results are reported in the following table:
as can be seen from the test results in the table above,
firstly, for tobacco mosaic virus, the detoxification rate of the detoxified ginger stock seed in the examples 1-3 is as high as 100%, and the detoxification rate of the detoxified ginger stock seed in the comparative examples 4-6 is only 93.7% at most;
② for the cucumber mosaic virus, the detoxification rate of the detoxified ginger stock seed in the embodiment 1-3 is up to 100%, while the detoxification rate of the detoxified ginger stock seed in the comparative example 4-6 is up to 94.3%;
in summary, the 3, 5-dicarbethoxy-2, 6-dimethyl-1, 4-dihydropyridine is mixed into the substrate planted at the stem tip, so that the bacteriostatic effect of ginger on tobacco mosaic virus and cucumber mosaic virus can be improved.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (10)
1. The production method of the detoxified ginger original seed is characterized by comprising the following steps:
s1, burying disease-free ginger blocks in wet fine sand, culturing at room temperature, taking 1cm stem tips when the buds grow to 1-2cm, sterilizing, stripping the stem tips with 1-2 leaf primordia, and inoculating to a prepared culture medium;
s2, placing the culture medium in an insect-proof net room for culture;
s3, when the sprouts at the stem tips grow to 3-5 cm in height and have 4-5 leaves, planting the sprouts in a matrix for cultivation, spraying an aspirin aqueous solution after the planting is finished, and covering a mulching film, wherein the matrix is mixed with 3, 5-dicarbethoxy-2, 6-dimethyl-1, 4-dihydropyridine;
s4, shading by using a shading net when ginger seedlings emerge 20 days after cultivation, additionally fertilizing 25-30 kg of urea, removing the shading net before and after 'early autumn', removing a mulching film for additionally fertilizing 110 kg of compound fertilizer and 50-60 kg of potassium fertilizer in a ditch 15-17 cm away from the plant base, then covering soil, sealing the ditch and ridging, and finally watering thoroughly;
s5, erecting an arch shed above the ginger seedlings before blooming to prolong the growth of the ginger for 20-30 days, and harvesting to obtain the virus-free ginger stock seeds.
2. The method of claim 1, wherein the step of processing the fine wet sand at S1 comprises: soaking the fine sand in 800 times of 50% carbendazim solution.
3. The method for producing a detoxified ginger stock as claimed in claim 1, wherein the culture medium in S1 is MS + 3% sucrose + 0.5% agar +1mg/L KT kinetin +1mg/L naphthaleneacetic acid, and the pH is adjusted to 5.8.
4. The method for producing a detoxified ginger stock as set forth in claim 1, wherein the conditions for cultivation in step S2 are: culturing under the conditions of 12 hours/day of illumination, 4000 lux of light intensity and 26 +/-2 ℃.
5. The method for producing a detoxified ginger stock according to claim 1, wherein the distribution of the matrix in S3 is: the lower layer is nutrient soil, the middle layer is river sand and the upper layer is vermiculite.
6. The method for producing the detoxified ginger stock seed according to claim 5, wherein the vermiculite is evenly sprinkled with water before use, so that the water holding capacity of the vermiculite reaches a saturation state.
7. The method for producing the detoxified ginger stock seed according to claim 1, wherein in S3, before covering with mulching film, spraying with herbicide and water is required.
8. The method for producing the detoxified ginger stock seed according to claim 1, wherein the ratio of aspirin to water in the aspirin-containing aqueous solution in S3 is 1:1000, and the aspirin is ground powder.
9. The method for producing the detoxified ginger stock seed according to claim 1, wherein the amount of 3, 5-dicarbethoxy-2, 6-dimethyl-1, 4-dihydropyridine in S3 is 3-5% by mass of the matrix.
10. The method of claim 1, wherein the S4 step of removing the sunshade net is followed by 1 water pour every 7-8 days.
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