CN114324910A - Biological detection chip, biological detection device and refrigerator - Google Patents

Abstract

The invention relates to a biological detection chip, a biological detection device and a refrigerator, wherein the biological detection chip is used for qualitatively and/or quantitatively detecting preset detection parameters of a sample, and comprises a plurality of detection channels which are arranged in parallel and are mutually independent, and each detection channel is provided with a detection chamber for containing a detection reagent. From this, a plurality of detecting channel do not influence each other, and every detecting channel all can detect one of them detection parameter of a sample independently, and a plurality of detecting channel can detect a plurality of different parameters of a plurality of different samples or same sample simultaneously, have saved and have detected required material, have reduced check-out time, have simplified the testing process, have improved detection efficiency, have promoted user's use and have experienced.

Description

Biological detection chip, biological detection device and refrigerator

Technical Field

The invention relates to a refrigeration and freezing technology, in particular to a biological detection chip, a biological detection device and a refrigerator.

Background

With the improvement of living standard of people, pesticide residues, viruses, nutrient elements or other aspects of edible food materials are generally required to be detected in daily life so as to qualitatively or quantitatively acquire the conditions of the food materials. For example, due to the abuse problem of pesticides, fruits, vegetables and agricultural and sideline products purchased daily by people may have the problem of excessive pesticide residue content, and if the problem of excessive pesticide residue content of the foods cannot be found in time, the foods can cause great harm after being taken by human bodies. As another example, breast feeding advocated at present is the best for infants only when breast milk has normal nutritional value, but in cases of lactating mothers suffering from illness, taking medicine, surgery or other conditions, the nutritional content of milk secreted by the mothers may be reduced and viruses may be produced, thereby affecting the growth and health of the infants.

Among the detection methods, the method of detecting by using the biological detection chip is rapid, small in size and suitable for families. However, the existing biological detection chip only comprises one detection flow path, one biological detection chip can only detect one parameter of one sample at a time, and when a user needs to detect a plurality of different samples or different parameters of the same sample, a plurality of biological detection chips are needed to be used for multiple detections, which wastes time and material, is high in cost, needs to be replaced for multiple times, is complex to operate, and has poor user experience effect.

Disclosure of Invention

It is an object of the first aspect of the present invention to overcome at least one of the drawbacks of the prior art by providing a bioassay chip capable of simultaneously detecting a plurality of different samples or a plurality of different parameters of the same sample.

It is a further object of the first aspect of the present invention to improve the operational convenience of the user in using the bioassay chip.

It is an object of the second aspect of the present invention to provide a bioassay system for mounting the bioassay chip according to any one of the above aspects.

It is an object of a third aspect of the present invention to provide a refrigerator having the biological detection apparatus according to any of the above aspects.

According to a first aspect of the present invention, there is provided a bioassay chip for qualitatively and/or quantitatively detecting a predetermined detection parameter of a sample, wherein

The biological detection chip comprises a plurality of detection channels which are arranged in parallel and are mutually independent, and each detection channel is provided with a detection chamber for containing a detection reagent.

Optionally, each of the detection channels further comprises a sampling chamber for providing a sample liquid to the detection chamber of the detection channel, and the sampling chambers of the detection channels are independent from each other.

Optionally, an extraction fluid is predisposed in the sampling chamber to mix with the extraction fluid after the sample is placed in the sampling chamber to produce a sample fluid.

Optionally, each of the detection channels further comprises a reaction chamber for containing a reaction reagent, wherein the reaction chamber is located between the sampling chamber and the detection chamber, so as to allow the sample liquid in the sampling chamber to flow into the detection chamber after flowing through the reaction chamber.

Optionally, the reaction reagent is preset in the reaction chamber; and/or

The reaction reagent is colloidal gold attached to the inner wall of the reaction chamber.

Optionally, the detection reagent is preset in the detection chamber; and/or

The detection chamber is internally provided with test paper, the detection reagent is integrated on the test paper, and at least one side of the detection chamber is open or transparent.

Optionally, a plurality of the detection channels are arranged side by side along the width direction of the biological detection chip, and each detection channel extends along the length direction of the biological detection chip; and is

The detection chambers of the plurality of detection channels are positioned at the same side of the biological detection chip in the length direction of the biological detection chip.

According to a second aspect of the present invention, there is also provided a bioassay device for mounting the bioassay chip according to any one of the above aspects, the bioassay device including:

and the chip mounting part is provided with a plurality of fluid interfaces which are respectively in one-to-one correspondence with a plurality of detection channels of the biological detection chip, so that the biological detection chip is mounted to the chip mounting part and then each fluid interface is communicated with the corresponding detection channel.

Optionally, the biological detection apparatus further comprises:

a drive mechanism having an output port;

the inside of chip installation department still is formed with interface channel, interface channel's one end with output port intercommunication, the other end has a plurality of branch passageways, and is a plurality of branch passageway communicates with a plurality of fluid interface one-to-one ground respectively, with through actuating mechanism is simultaneously to installing in the chip installation department fluid in a plurality of detection channel of biological detection chip drives.

According to a third aspect of the present invention, there is also provided a refrigerator comprising the biological detection apparatus according to any one of the above aspects.

The biological detection chip provided by the invention is provided with a plurality of parallel mutually independent detection channels, and each detection channel is provided with a detection chamber for accommodating a detection reagent, so that the detection channels are not influenced mutually, each detection channel can independently detect one detection parameter of a sample, and the detection channels can simultaneously detect a plurality of different samples or a plurality of different parameters of the same sample, thereby saving materials required by detection, reducing detection time, simplifying detection process, improving detection efficiency and improving use experience of users.

Furthermore, the extracting solution is preset in the sampling chamber, when the sampling device is used, only the sample needs to be placed into the sampling chamber, and the substance to be detected on the sample is dissolved in the extracting solution to form sample solution with proper concentration, so that the complex operation of manually preparing the sample solution by a user is omitted, and the operation convenience of detecting by using the biological detection chip by the user is improved.

The above and other objects, advantages and features of the present invention will become more apparent to those skilled in the art from the following detailed description of specific embodiments thereof, taken in conjunction with the accompanying drawings.

Drawings

Some specific embodiments of the invention will be described in detail hereinafter, by way of illustration and not limitation, with reference to the accompanying drawings. The same reference numbers in the drawings identify the same or similar elements or components. Those skilled in the art will appreciate that the drawings are not necessarily drawn to scale. In the drawings:

FIG. 1 is a schematic structural view of a bioassay chip according to an embodiment of the present invention;

FIGS. 2 and 3 are schematic cross-sectional views of a bioassay chip according to an embodiment of the present invention, taken along different cross-sectional planes, respectively;

FIG. 4 is a schematic structural view of a biological detection apparatus according to one embodiment of the present invention;

FIG. 5 is a schematic exploded view of a biological testing device according to one embodiment of the present invention;

FIG. 6 is a schematic cross-sectional view of a biological testing device according to one embodiment of the present invention;

FIG. 7 is a schematic front perspective view of a biological detection device according to one embodiment of the invention;

FIG. 8 is a schematic sectional view after a bioassay chip according to an embodiment of the present invention is mounted to a bioassay device;

fig. 9 is a schematic enlarged view of a portion a in fig. 8;

FIG. 10 is a schematic flow chart of a control method of a bio-detection apparatus according to an embodiment of the present invention;

fig. 11 is a schematic structural view of a refrigerator according to one embodiment of the present invention;

fig. 12 is a schematic exploded view of a door body according to an embodiment of the present invention.

Detailed Description

The invention firstly provides a biological detection chip which is used for qualitatively and/or quantitatively detecting the preset detection parameters of a sample. The preset detection parameter may be, for example, an agricultural residue parameter indicating whether the agricultural residue exceeds the standard and/or a specific value of the agricultural residue, a nutrient parameter indicating whether the nutrient element meets the standard and/or a specific content of the nutrient element, a specific substance parameter indicating whether a specific harmful substance (e.g., a specific virus) exceeds the standard and/or a specific content, and the like.

FIG. 1 is a schematic structural view of a bioassay chip according to an embodiment of the present invention, and FIGS. 2 and 3 are schematic sectional views of the bioassay chip according to an embodiment of the present invention, taken along different sectional planes, respectively. Referring to FIGS. 1 to 3, the bioassay chip 2 of the present invention includes a plurality of parallel-arranged and independent detection channels 20, each of the detection channels 20 having a detection chamber 23 for containing a detection reagent. It should be noted that the parallel arrangement means that the flow channels in the detection channels 20 are isolated and independent from each other, and the fluid in each detection channel does not flow through the other detection channels. That is, the detection channels 20 are not affected by each other, each detection channel 20 can independently detect one of the detection parameters of one sample, and the detection channels 20 can simultaneously detect a plurality of different samples or a plurality of different parameters of the same sample. Compared with the traditional biological detection chip which is only provided with one detection channel, the biological detection chip 2 saves materials required by detection, reduces the times of installing and replacing the chip by a user, thereby reducing the detection time, simplifying the detection process, improving the detection efficiency and improving the use experience of the user.

In some embodiments, a plurality of detection channels 20 are arranged side by side along the width direction of the bio-detection chip 2, and each detection channel 20 extends along the length direction of the bio-detection chip 2, so that the layout on the bio-detection chip 2 is more compact, thereby reducing the volume of the bio-detection chip 2 and making it more suitable for being integrated into a home appliance.

Further, the detection chambers 23 of the plurality of detection channels 20 are located at the same side of the biological detection chip 2 in the length direction thereof, so that the user can observe the detection results of the plurality of detection channels 20 at the same time, which is in accordance with the usage habit of the user.

Specifically, the bioassay chip 2 further includes a body 40, and each of the detecting channels 20 is formed on the body 40. In the embodiment shown in fig. 1, the body 40 is substantially rectangular parallelepiped, and the plurality of detection chambers 23 may be disposed adjacent to the bottom of the body 40 and laterally side by side.

In some embodiments, each detection channel 20 further comprises a sampling chamber 21 for providing a sample fluid to the detection chamber 23 of the detection channel, the sampling chambers 21 of the respective detection channels 20 being independent of each other. That is, each detection channel 20 is independently sampled to allow each detection channel 20 to have a different sample fluid, e.g., the sample fluids of each detection channel 20 may differ in type, concentration, or other aspects. Specifically, in the embodiment shown in FIG. 1, a plurality of sampling chambers 21 can be located adjacent the top of the body 40 and laterally side-by-side.

Further, an extraction liquid is preset in the sampling chamber 21 to be mixed with the extraction liquid therein after the sample is set in the sampling chamber 21 to generate a sample liquid. When the sample liquid sampling device is used, a user only needs to put a sample into the sampling chamber, and a substance to be detected on the sample is dissolved into the extracting solution to form sample liquid with proper concentration, so that the complex operation of manually preparing the sample liquid by the user is omitted, and the operation convenience of detecting by using the biological detection chip 2 by the user is improved. The kind and amount of the extraction liquid in the sampling chambers 21 of the different detection channels 20 may be the same or different as necessary. In some alternative embodiments, the extraction liquid may also be added to the sampling chamber 21 of the bioassay chip 2 during use thereof.

In some embodiments, each detection channel 20 further comprises a reaction chamber 22 for containing a reaction reagent, and the reaction chamber 22 is located between the sampling chamber 21 and the detection chamber 23 to allow the sample liquid in the sampling chamber 21 to flow into the detection chamber 23 after flowing through the reaction chamber 22. That is, the reaction chamber 22 is downstream of the sampling chamber 21 and upstream of the detection chamber 23 in the fluid flow direction in the detection channel 20. For a specific sample solution or for some specific detection parameters of the sample solution, the sample solution needs to be reacted with a reaction reagent first and then reacted with a detection reagent, and the reaction reagent and the detection reagent are respectively arranged in the reaction chamber 22 and the detection chamber 23 which are different in position, so that the reaction or mutual influence between the reaction reagent and the detection reagent can be avoided, and the accuracy of the detection result is improved.

In some embodiments, the reagent is pre-positioned in the reaction chamber 22, so as to avoid the operation trouble caused by adding the reagent manually by a user or avoid the complicated structure caused by automatically adding the reagent by using a driving mechanism.

In some embodiments, the reaction reagent may be colloidal gold attached to the inner walls of the reaction chamber 22. The colloidal gold support arranged in this way is stable and not easy to move, so that the colloidal gold support is not easy to influence the sampling chamber 21 or the detection chamber 23 and is not easy to be influenced by the reagent in the sampling chamber 21 or the detection chamber 23.

In some embodiments, the detection reagent is pre-set in the detection chamber 23 to avoid the operation trouble of manually adding the detection reagent by a user or the structural complexity caused by automatically adding the detection reagent by using a driving mechanism.

In some embodiments, a test strip 60 is disposed in the detection chamber 23, the detection reagent is integrated on the test strip 60, and at least one side of the detection chamber 23 is open or transparent to facilitate observation of the detection result on the test strip 60 by a user.

Of course, in some alternative embodiments, the reaction reagent and the detection reagent may be added to the reaction chamber 22 and the detection chamber 23, respectively, when the bioassay chip 2 is used.

In some embodiments, the sampling chamber 21, the reaction chamber 22, and the detection chamber 23 of each detection channel 20 may be in communication in sequence through a microchannel. The fluids in the sampling chamber 21, the reaction chamber 22 and the detection chamber 23 can be kept at the set position by the micro flow channel without flowing to other positions under the action of no external driving force. For example, the flow of the extraction liquid in the sampling chamber 21 through the microchannel does not affect the reagent in the reaction chamber 22 or the detection chamber 23 by flowing to the reaction chamber 22 or the detection chamber 23.

In some preferred embodiments, the sampling chamber 21, the reaction chamber 22 and the detection chamber 23 of each detection channel 20 can also be isolated from each other. Only after the bioassay chip 2 is mounted to its mounting position, the sampling chamber 21, the reaction chamber 22 and the detection chamber 23 of each detection channel 20 are communicated in sequence in cooperation with the structure of its mounting position. Therefore, even if the sampling chamber 21, the reaction chamber 22 and the detection chamber 23 are pre-filled with reagents of corresponding types, no matter whether the reagents are in a gas state, a liquid state or a solid state, the mutual influence among the sampling chamber 21, the reaction chamber 22 and the detection chamber 23 can be effectively avoided, and the biological detection chip 2 can be stored for a long time on the premise of not losing efficacy.

The present invention also provides a bioassay system 1 for mounting the bioassay chip 2 according to any of the above-described embodiments. Fig. 4 is a schematic structural view of a biological detection apparatus according to an embodiment of the present invention, fig. 5 is a schematic structural exploded view of the biological detection apparatus according to an embodiment of the present invention, and fig. 6 is a schematic sectional view of the biological detection apparatus according to an embodiment of the present invention. FIG. 7 is a schematic front perspective view of a biological detection device according to one embodiment of the invention.

The bioassay system 1 includes a chip mounting unit 11, and a plurality of fluid ports 111 are formed in the chip mounting unit 11, which correspond to the plurality of detection channels 20 of the bioassay chip 2 one by one, respectively, so that each of the fluid ports 111 communicates with a corresponding one of the detection channels 20 after the bioassay chip 2 is mounted on the chip mounting unit 11. Thereby, it is facilitated to control the fluid flow of each detection channel 20 through the fluidic interface 111 or to add reagents or the like into each detection channel 20 through the fluidic interface 111. Therefore, the biological detection device 1 can simultaneously cooperate with a plurality of detection channels 20 of the biological detection chip 1 to simultaneously detect a plurality of different samples or a plurality of different parameters of the same sample.

In some embodiments, the biological detection apparatus 1 further includes a drive mechanism 30, the drive mechanism 30 having an output port 341. The chip mounting part 11 is further formed with a connection channel 12 therein, one end of the connection channel 12 communicates with the output port 341, and the other end has a plurality of branch channels 121, and the plurality of branch channels 121 communicate with the plurality of fluid ports 111 in a one-to-one correspondence, respectively, to simultaneously drive the fluid in the plurality of detection channels 20 of the bioassay chip 2 mounted in the chip mounting part 11 by the driving mechanism 30. Each of the branch passages 121 may be connected to the output port 341 through an inclined passage 122 formed inside the holder 10.

This application will be used for communicating the interface channel 12 design between actuating mechanism 30 and each fluid interface 111 inside chip installation department 11, has avoided external connecting tube to lead to arranging the problem that the degree of difficulty is big, bulky, and produce the interference with other structures easily between actuating mechanism 30 and each fluid interface 111, has simplified the structure of biological detection device 1, makes it be applicable to more on integrated refrigerator or other domestic appliance.

Specifically, the chip mounting portion 11 may be formed on the holder 10 of the bioassay device 1 with the driving mechanism 30 supported on the holder 10.

In some embodiments, the drive mechanism 30 is a micro-syringe pump to induce fluid flow therein by pressurizing air into the detection channel. When the driving mechanism 30 injects air into the detection channel, the pressure in the detection channel increases, and the fluid in the detection channel flows under the action of the pressure difference between the inside and the outside of the detection channel.

Specifically, the driving mechanism 30 may include a driving motor 31, a lead screw 32, a slider 33, an injector 34, and a piston 35. The drive motor 31 is used to output a drive force. The lead screw 32 is connected to the driving motor 31 to be rotated by the driving motor 31. The sliding block 33 is arranged on the screw rod 32 in a penetrating way and is in threaded connection with the screw rod 32 so as to translate along the screw rod 32 along with the rotation of the screw rod 32. A first end of the syringe 34 communicates with the connecting passage 12. The piston 35 is disposed inside the syringe 34 and is fixedly connected to the slider 33 to move inside the syringe 34 under the driving of the slider 33, so as to promote the fluid flow inside the detection channel when moving toward the first end of the syringe 34.

Further, one end of the driving motor 31 facing away from the screw rod 32 and a circumferential side of the driving motor 31 are both spaced apart from the bracket 10. That is, the driving motor 31 is not in contact with the support 10 except for the end fixedly connected to the support 10. Therefore, sufficient space is provided for heat dissipation of the driving motor 31, and timely dissipation of heat generated by the driving motor is facilitated. Further, the transmission of the vibration generated when the driving motor 31 is operated to other components (for example, a biological detection chip mounted on a chip mounting portion) on the holder 10 is reduced to some extent, and the influence on the other components is avoided.

Because the preparation of the sample liquid and the reaction between the sample liquid and the reaction reagent require time, and the detection channel of the biological detection chip is through after the biological detection chip is installed, the flow position and the flow time of the sample liquid in the detection channel need to be accurately controlled to ensure the accuracy of the detection result. That is, the amount and time of movement of the piston 35 during operation of the drive mechanism 30 are critical.

To this end, in some embodiments, the biological detection apparatus 1 further includes a position sensor 92, and the position sensor 92 is used for detecting the position of the slide block 33 to control the operation of the driving motor 31 according to the position of the slide block 33, so as to control the path of the fluid flow in the detection channel by controlling the displacement of the piston 35 in the injector 34, thereby facilitating the monitoring of the position of the piston 35 and the displacement of the movement thereof, and further realizing the precise control of the fluid flow path.

In some embodiments, the biological detection device 1 further includes a circuit board 93, the circuit board 93 being fixed to the support 10. The position sensor 92 may be disposed on the circuit board 93 and electrically connected to the circuit board 93. Specifically, the circuit board 93 may be fixed to an upper portion of the bracket 10 at a front side of the driving mechanism 30. The upper portion of the bracket 10 may be forwardly extended with a plurality of claws 14 to thereby snap-fit the circuit board 93 thereto.

In some embodiments, the biological detection apparatus 1 further includes a front cover 91, and the front cover 91 covers the front side of the support 10, so as to cover at least a part of the structure of the support 10 and prevent the support 10, the circuit board 93 mounted on the support 10, the driving mechanism 30, and the like from being exposed to the front side of the biological detection apparatus 1 to affect the appearance thereof.

Further, a notch 911 is formed in the front cover 91, and the chip mounting portion 11 is exposed to the front side of the front cover 91 through the notch 911, so that the bioassay chip is mounted to the chip mounting portion 11 through the notch 911.

FIG. 8 is a schematic cross-sectional view of a bioassay chip according to an embodiment of the present invention after being mounted to a bioassay device, and FIG. 9 is a schematic enlarged view of a portion A in FIG. 8. In some embodiments, the sampling chamber 21, the reaction chamber 22, and the detection chamber 23 of each detection channel 20 are isolated from each other. Only after the bioassay chip 2 is mounted on the chip mounting part 11, the sampling chamber 21, the reaction chamber 22 and the detection chamber 23 of each detection channel 20 are communicated in sequence by the structural cooperation of the chip mounting part 11.

Specifically, at least a part of the peripheral walls of the sampling chamber 21 and the reaction chamber 22 of each detection channel 20 are permeable walls to form at least one penetration hole under the action of the piercing structure on the chip mounting portion 11. The chip mounting part 11 is provided with a first piercing structure 51 having a first through passage 511 formed therein for piercing the penetrable wall 221 of the reaction chamber 22 and the penetrable wall 211 of the sampling chamber 21 of the same detection passage 20 after the bioassay chip 2 is mounted to the chip mounting part 11 and allowing fluid communication between the reaction chamber 22 and the sampling chamber 21 of the same detection passage 20 through the first through passage 511.

Further, the detection chamber 23 of each detection channel 20 has a liquid injection port 231 communicating with the inside thereof. The chip mounting part 11 is further provided with a second piercing structure 52 having a second through-passage 521 formed therein for piercing the penetrable wall 222 of the corresponding reaction chamber 22 after the bioassay chip 2 is mounted to the chip mounting part 11 and allowing the reaction chamber 22 to communicate with the liquid injection port 231 of the corresponding detection chamber 23 through the second through-passage 521.

That is, a first piercing structure 51 can be used to pierce and provide fluid communication between the penetrable walls of sampling chamber 21 and reaction chamber 22, and a second piercing structure 52 can be used to pierce and provide fluid communication between reaction chamber 22 and detection chamber 23. Therefore, complicated connecting pipelines are omitted, the structure is simplified, and the sampling chamber 21, the reaction chamber 22 and the detection chamber 23 of the biological detection chip 2 can be allowed to be arranged at intervals, thereby being beneficial to long-term storage of the biological detection chip 2 and allowing liquid reagents to be pre-stored in the sampling chamber, the reaction chamber or the detection chamber.

In some embodiments, the chip mounting portion 11 further has a plurality of sample-loading structures 70 corresponding to the sampling chambers 21 one by one, and each sample-loading structure 70 is configured to push the solid sample resting outside the corresponding sampling chamber 21 into the sampling chamber 21 when the biological detection chip 2 is mounted on the chip mounting portion 11, so that the solid sample is soaked in the extracting solution in the sampling chamber 21. The substance to be detected on the solid sample dissolves into the extracting solution to generate a sample solution. From this, can accomplish the application of sample operation automatically in the installation of biological detection chip 2, the user only need shelve solid-state sample outside sampling chamber 21 to directly install biological detection chip 2 can, need not other operations, also need not to set up the actuating mechanism of other supplementary applications of sample on biological detection device 1, simplified biological detection device 1's structure, reduced its cost, improved user's use and experienced, make it more suitable for the family to use.

Correspondingly, the outer side of each sampling chamber 21 is provided with a sampling port 24 for holding a solid sample, and the sampling ports 24 correspond to the positions of the sample adding structures 70 of the biological detection device 1 one by one, so as to allow each sample adding structure 70 to push the solid sample held at the corresponding sampling port 24 into the corresponding sampling chamber 21 when the biological detection chip 2 is mounted on the biological detection device 1. The sampling port 24 is separated from the sampling chamber 21 by another permeable wall 212 of the sampling chamber 21, and the sample loading structure 70 can penetrate the permeable wall 212 to push the sample into the sampling chamber 21.

Specifically, the sampling port 24 may be recessed toward the direction in which the sampling chamber 21 is located, so as to hold the solid sample therein and prevent the solid sample from falling. The plurality of thief hatches 24 may be spaced apart from one another, thereby forming a plurality of individual recesses. The plurality of thief hatches 24 may also be left unseated to form a complete recess.

The chip mounting portion 11 is further provided with a connection column which is in one-to-one correspondence with the sample adding structure 70 and is arranged adjacently, and the branch channel 121 is formed inside the connection column. After the bioassay chip 2 is mounted on the chip mounting portion 11, the penetrating wall 213 at the top of the corresponding sampling well 21 is penetrated by the connecting column, so that the branch passage 121 in the connecting column communicates with the corresponding sampling well 21.

The following describes in detail a control method for controlling the bio-detection chip mounted thereon using the bio-detection apparatus. Fig. 10 is a schematic flowchart of a control method of a bio-detection apparatus according to an embodiment of the present invention, which may include:

a step S10 of receiving a first trigger signal indicating that the bioassay chip 2 has been mounted on the chip mounting part 11;

step S20, waiting for a first preset time to fully dissolve the substance to be detected on the sample into the extracting solution in the sampling chamber 21, so as to form a sample solution with a proper concentration;

step S31, activating the driving mechanism 30 to drive the sample liquid in the sampling chamber 21 of each detection channel 20 to flow to the reaction chamber 22 of the detection channel through the driving mechanism 30;

step S32, determining whether the sample liquid flowing into the reaction chamber 22 reaches a preset sample amount; if yes, go to step S33; in this step, the position of the piston is detected, and when the piston moves to the preset position, it indicates that the sample liquid flowing into the reaction chamber 22 has reached the preset sample amount;

step S33, stopping the drive mechanism 30;

step S34, when the stopping time of the driving mechanism 30 reaches a second preset time, the driving mechanism 30 is started again to promote the sample liquid in the reaction chamber 22 of each detection channel 20 to flow to the detection chamber 23 of the detection channel 20;

and step S40, sending out prompt information for prompting that the detection result is shown after waiting for a third preset time.

Further, the control method of the present invention further includes:

when receiving the second trigger signal for instructing the removal of the bio-detection chip 2 from the chip mounting part 11, the piston controlling the driving mechanism 30 is restored to the initial position.

The present invention also provides a refrigerator, and fig. 11 is a schematic structural view of a refrigerator according to an embodiment of the present invention. The refrigerator 100 of the present invention includes the biological detection device 1 according to any of the above-described embodiments to integrate the biological detection device 1 into the refrigerator 100. The refrigerator 100 may further include a bio-detection chip 2 mounted on the bio-detection apparatus 1. The refrigerator 100 is frequently used in daily life, and the refrigerator 100 is mainly used for storing food materials, so that when the biological detection device 1 is integrated into the refrigerator 100, a user can conveniently perform a detection operation of a food material sample by using the biological detection device 1.

Further, the refrigerator 100 further includes a cabinet 200 and a door 300, wherein the cabinet 200 defines a storage space therein, and the door 300 is connected to the cabinet 200 and is used for opening and/or closing the storage space. The biological detection device 1 is preferably disposed on the door 300, which not only is convenient to operate, but also does not occupy the original storage space in the refrigerator body 200, and does not affect the storage capacity of the refrigerator 100.

Fig. 12 is a schematic exploded view of a door body according to an embodiment of the present invention. In some embodiments, the hollow window 301 is disposed at the front side of the door 300, and the chip mounting portion 11 is exposed to the front side of the door 300 through the hollow window 301, so that a user can mount the biological detection chip 2 to implement a detection function without opening the door 300, thereby ensuring the heat preservation performance of the refrigerator 100 and saving energy consumption.

Specifically, the door body 300 may include a panel 302 for forming a front portion thereof, a door liner 303 for forming a rear portion thereof, and a foam insulation layer (not shown) disposed between the panel 302 and the door liner 303, and the cutout window 301 is opened on the panel 302. An embedded box 304 is embedded between the panel 302 and the door liner 303 before the foaming heat-insulating layer is formed, and the biological detection device 1 is arranged in the embedded box 304. That is, the cassette 304 is provided in advance between the panel 302 and the door liner 303 before the door 300 is foamed, for reserving a space for installing the biological detection apparatus 1 between the panel 302 and the door liner 303.

Further, the embedded box 304 is attached to the rear surface of the panel 302, the front side of the embedded box 304 is open and faces the hollow window 301, so that the biological detection device 1 is allowed to be installed in the embedded box 304 from front to back through the hollow window 301, and the installation convenience of the biological detection device 1 is improved.

Further, the mounting posture of the biological detection chip 2 in the chip mounting portion 11 is set so that each detection channel 20 formed extends in the vertical direction. Thus, it is convenient for the user to install the biological detection chip 2, and it conforms to the operation habit of the user.

The refrigerator 100 of the present application is a refrigerator in a broad sense, and includes not only a so-called refrigerator in a narrow sense but also a storage device having a refrigerating, freezing or other storage function, for example, a refrigerator, a freezer, and the like.

It should be further understood by those skilled in the art that the terms "upper", "lower", "front", "rear", "top", "bottom", and the like used in the embodiments of the present invention are used with reference to the actual usage state of the bio-detection apparatus 1 and the refrigerator 100, and these terms are only used for convenience of description and understanding of the technical solutions of the present invention, and do not indicate or imply that the apparatus referred to must have a specific orientation, be configured and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

Thus, it should be appreciated by those skilled in the art that while a number of exemplary embodiments of the invention have been illustrated and described in detail herein, many other variations or modifications consistent with the principles of the invention may be directly determined or derived from the disclosure of the present invention without departing from the spirit and scope of the invention. Accordingly, the scope of the invention should be understood and interpreted to cover all such other variations or modifications.

Claims (10)

1. A biological detection chip for qualitatively and/or quantitatively detecting a preset detection parameter of a sample,

the biological detection chip comprises a plurality of detection channels which are arranged in parallel and are mutually independent, and each detection channel is provided with a detection chamber for containing a detection reagent.

2. The bioassay chip as set forth in claim 1,

each detection channel further comprises a sampling chamber for providing a sample liquid to the detection chamber of the detection channel, and the sampling chambers of the detection channels are independent from each other.

3. The bioassay chip as set forth in claim 2,

an extraction liquid is preset in the sampling chamber to be mixed with the extraction liquid after a sample is placed in the sampling chamber so as to generate a sample liquid.

4. The bioassay chip as set forth in claim 2,

each detection channel further comprises a reaction chamber for containing a reaction reagent, wherein the reaction chamber is positioned between the sampling chamber and the detection chamber, so that the sample liquid in the sampling chamber flows into the detection chamber after flowing through the reaction chamber.

5. The bioassay chip as set forth in claim 4,

the reaction reagent is preset in the reaction chamber; and/or

The reaction reagent is colloidal gold attached to the inner wall of the reaction chamber.

6. The bioassay chip as set forth in claim 1,

the detection reagent is preset in the detection chamber; and/or

The detection chamber is internally provided with test paper, the detection reagent is integrated on the test paper, and at least one side of the detection chamber is open or transparent.

7. The bioassay chip as set forth in claim 1,

the detection channels are arranged side by side along the width direction of the biological detection chip, and each detection channel extends along the length direction of the biological detection chip; and is

The detection chambers of the plurality of detection channels are positioned at the same side of the biological detection chip in the length direction of the biological detection chip.

8. A bioassay system for mounting the bioassay chip according to any one of claims 1 to 7, comprising:

and the chip mounting part is provided with a plurality of fluid interfaces which are respectively in one-to-one correspondence with a plurality of detection channels of the biological detection chip, so that the biological detection chip is mounted to the chip mounting part and then each fluid interface is communicated with the corresponding detection channel.

9. The biological detection device of claim 8, further comprising:

a drive mechanism having an output port;

the inside of chip installation department still is formed with interface channel, interface channel's one end with output port intercommunication, the other end has a plurality of branch passageways, and is a plurality of branch passageway communicates with a plurality of fluid interface one-to-one ground respectively, with through actuating mechanism is simultaneously to installing in the chip installation department fluid in a plurality of detection channel of biological detection chip drives.

10. A refrigerator comprising the biological detection device according to any one of claims 8 to 9.

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