CN114324913A

CN114324913A - Micro-fluidic detection system for refrigerator and refrigerator

Info

Publication number: CN114324913A
Application number: CN202011030041.7A
Authority: CN
Inventors: 刘浩泉; 赵斌堂; 吕守鹏; 臧艺强
Original assignee: Qingdao Haier Refrigerator Co Ltd; Haier Smart Home Co Ltd
Current assignee: Qingdao Haier Refrigerator Co Ltd; Haier Smart Home Co Ltd
Priority date: 2020-09-27
Filing date: 2020-09-27
Publication date: 2022-04-12

Abstract

The invention relates to a micro-fluidic detection system for a refrigerator and the refrigerator, wherein the micro-fluidic detection system comprises: the microfluidic biochip is provided with a sample inlet, a communication port and a detection pool formed inside the microfluidic biochip, and the sample inlet, the detection pool and the communication port are sequentially communicated through a micro-channel; the sample liquid driving device is communicated with the communication port and is used for promoting the sample liquid contacted with the sample injection port to enter the micro-channel and flow to the detection pool through the micro-channel; the sealed docking mechanism is used for forming fluid sealed connection between the microfluidic biochip and the sample liquid driving device; and the detection mechanism is used for detecting the detection pool so as to obtain the preset detection parameters of the sample liquid. Therefore, the normal sample introduction effect of the micro-fluidic detection system is ensured, and the sample introduction is conveniently and accurately controlled by using the sample liquid driving device.

Description

Micro-fluidic detection system for refrigerator and refrigerator

Technical Field

The invention relates to a refrigeration and freezing technology, in particular to a micro-fluidic detection system for a refrigerator and the refrigerator.

Background

With the improvement of living standard of people, pesticide residues, viruses, nutrient elements or other aspects of edible food materials are generally required to be detected in daily life so as to qualitatively or quantitatively acquire the conditions of the food materials. For example, due to the abuse problem of pesticides, fruits, vegetables and agricultural and sideline products purchased daily by people may have the problem of excessive pesticide residue content, and if the problem of excessive pesticide residue content of the foods cannot be found in time, the foods can cause great harm after being taken by human bodies. As another example, breast feeding advocated at present is the best for infants only when breast milk has normal nutritional value, but in cases of lactating mothers suffering from illness, taking medicine, surgery or other conditions, the nutritional content of milk secreted by the mothers may be reduced and viruses may be produced, thereby affecting the growth and health of the infants.

Among the detection methods, the method for detecting by utilizing the microfluidic biochip is relatively quick, small in size and suitable for being used at home. In order to make the sample introduction of the microfluidic biochip more accurate and convenient to control, a driving device can be used for driving the sample liquid to enter the microfluidic biochip, and at the moment, the effect and the accuracy of the driving sample introduction are directly influenced by the quality of the butt joint between the driving device and the microfluidic biochip.

Disclosure of Invention

An object of the first aspect of the present invention is to overcome at least one of the defects in the prior art, and to provide a micro-fluidic detection system with good sample injection effect and accurate sample injection control, which is suitable for a refrigerator.

It is a further object of the first aspect of the invention to improve the reliability and sealing effect of the sealed interface between the microfluidic biochip and the sample liquid driving means.

It is a further object of the first aspect of the invention to improve the robustness of microfluidic biochip mounting.

The second aspect of the invention aims to provide a refrigerator with the microfluidic detection system.

According to a first aspect of the present invention, there is provided a microfluidic detection system for a refrigerator, comprising:

the microfluidic biochip is provided with a sample inlet, a communication port and a detection pool formed inside the microfluidic biochip, wherein the sample inlet, the detection pool and the communication port are sequentially communicated through a micro-channel;

the sample liquid driving device is communicated with the communication port and is used for promoting the sample liquid contacted with the sample injection port to enter the micro-channel and flow to the detection pool through the micro-channel;

the sealed docking mechanism is used for forming fluid sealed connection between the microfluidic biochip and the sample liquid driving device; and

and the detection mechanism is used for detecting the detection cell so as to obtain the preset detection parameters of the sample liquid.

Optionally, the sealed docking mechanism comprises:

the sealing connecting piece is connected between the microfluidic biochip and the sample liquid driving device, and a connecting channel penetrating through the sealing connecting piece is formed in the sealing connecting piece; and

and the elastic pressing piece is used for applying elastic acting force to the sealing connecting piece so that the sealing connecting piece is simultaneously in sealing butt joint with the sample liquid driving device and the microfluidic biochip, and therefore the communication ports of the sample liquid driving device and the microfluidic biochip are in sealing communication through the connecting channel.

Optionally, the elastic pressing member is disposed between the sealing connector and the sample liquid driving device to apply an elastic force to the sealing connector towards the microfluidic biochip, so that the elastic force is used to urge the sealing connector to tightly and elastically abut against the microfluidic biochip, and a reaction force of the microfluidic biochip to the sealing connector is used to urge the sealing connector to elastically and hermetically abut against the sample liquid driving device.

Optionally, the sample liquid driving device is communicated with the communication port through a connecting pipeline;

the sealing connecting piece comprises a first connecting column which protrudes and extends towards the connecting pipeline, and the connecting channel penetrates through the first connecting column; the first connection column is inserted into the connection pipe and is in close contact with the inner wall of the connection pipe, so that the connection channel is in sealed communication with the connection pipe, and thus the connection channel is in sealed communication with the sample liquid driving device.

Optionally, the elastic pressing member includes a spring, one end of the spring abuts against a fixedly disposed end plate, the other end of the spring abuts against the sealing connector, and the end plate and the microfluidic biochip are respectively located on two opposite sides of the sealing connector.

Optionally, the sealing and butting structure further comprises a guide rod, and the spring is sleeved on the guide rod; and is

One end of the guide rod is fixedly connected with the sealing connecting piece, and the other end of the guide rod is in contact with a Hall switch after the microfluidic biochip is in sealing butt joint with the sealing connecting piece, so that the Hall switch is prompted to generate a trigger signal for indicating that the microfluidic biochip is installed in place.

Optionally, the sealing connector further comprises a first connecting block for directly interfacing with the microfluidic biochip and a second connecting block disposed on a side of the first connecting block facing away from the microfluidic biochip, and the first connecting column protrudes from the second connecting block to the connecting pipeline;

the inner part of the second connecting block is provided with a column hole, the side of the first connecting block, which deviates from the microfluidic biochip, is provided with a second connecting column, the second connecting column is plugged in the column hole, and the connecting channel penetrates through the first connecting column, the first connecting block and the second connecting block.

Optionally, the microfluidic detection system further comprises:

the clamping mechanism is used for clamping the microfluidic biochip to keep the microfluidic biochip in a fluid sealing connection state with the sample liquid driving device; wherein

The direction of the acting force generated by the clamping mechanism on the microfluidic biochip is perpendicular to the direction of the acting force generated by the sealing and butting mechanism on the microfluidic biochip.

Optionally, the clamping mechanism comprises two elastic clamping jaws arranged oppositely to apply opposite forces to the microfluidic biochip clamped between the two elastic clamping jaws; and is

The micro-fluidic detection system further comprises a cantilever key which is suspended on one side of the micro-fluidic biochip, the cantilever key is simultaneously abutted against the inner sides of the two elastic clamping jaws, and acting force which enables the elastic clamping jaws to elastically deform towards the direction deviating from each other is applied to the two elastic clamping jaws when the cantilever key is subjected to acting force towards the elastic clamping jaws, so that the clamping effect of the two elastic clamping jaws on the micro-fluidic biochip is relieved.

Optionally, the pesticide residue detection system further comprises:

the sample table is used for placing a sample cup, and the sample cup is used for containing a sample liquid; wherein

The sample stage comprises a supporting table for supporting a sample cup and an oscillator arranged on the supporting table, wherein the oscillator is used for oscillating the sample cup after the sample cup is placed on the supporting table, so that the buffer solution in the sample cup and the sample are fully mixed to generate the sample solution.

According to a second aspect of the present invention, there is also provided a refrigerator comprising the microfluidic detection system according to any of the above aspects.

The micro-fluidic detection system is particularly provided with the sealing butt-joint mechanism between the micro-fluidic biochip and the sample liquid driving device, and is used for realizing fluid sealing connection between the micro-fluidic biochip and the sample liquid driving device, avoiding the problems of air leakage, liquid leakage and the like at the joint between the micro-fluidic biochip and the sample liquid driving device from influencing the pressure in the main channel formed by the sequential communication of the sample inlet, the detection pool and the communication port, further influencing the sample liquid to enter the main channel, ensuring the normal sample introduction effect of the micro-fluidic detection system, and facilitating the accurate control of sample introduction by using the sample liquid driving device.

The sealing and butting mechanism is further designed to comprise a sealing connecting piece and an elastic pressing piece, and elastic acting force can be applied to the sealing connecting piece through the elastic pressing piece, so that the sealing connecting piece is always kept in a state of being tightly sealed and butted with the sample liquid driving device and the microfluidic biochip, the problems of looseness, breakage and the like caused by the fact that other butting mechanisms are adopted and long-term and reliable fluid sealing communication relation between the sample liquid driving device and the communication port of the microfluidic biochip is ensured, and the sealing effect between the sample liquid driving device and the communication port of the microfluidic biochip is improved.

Furthermore, the microfluidic biochip is clamped and fixed by the clamping mechanism, and the direction of the acting force generated by the clamping mechanism on the microfluidic biochip is perpendicular to the direction of the acting force generated by the sealing and butting mechanism on the microfluidic biochip, so that the microfluidic biochip is fixed and supported in two different directions, and the mounting stability of the microfluidic biochip is improved.

The above and other objects, advantages and features of the present invention will become more apparent to those skilled in the art from the following detailed description of specific embodiments thereof, taken in conjunction with the accompanying drawings.

Drawings

Some specific embodiments of the invention will be described in detail hereinafter, by way of illustration and not limitation, with reference to the accompanying drawings. The same reference numbers in the drawings identify the same or similar elements or components. Those skilled in the art will appreciate that the drawings are not necessarily drawn to scale. In the drawings:

FIG. 1 is a schematic block diagram of a microfluidic detection system for a refrigerator according to one embodiment of the present invention;

FIG. 2 is a schematic exploded view of a microfluidic detection system for a refrigerator according to one embodiment of the present invention;

FIG. 3 is a schematic block diagram of the internal structure of a microfluidic detection system according to one embodiment of the present invention;

FIG. 4 is a schematic exploded view of the internal structure of a microfluidic detection system according to one embodiment of the present invention;

FIG. 5 is a schematic block diagram of a microfluidic biochip according to one embodiment of the present invention;

fig. 6 is a schematic exploded view of a microfluidic biochip, a sample liquid driving apparatus, and a sealing and docking structure according to an embodiment of the present invention;

fig. 7 is a schematic cross-sectional view of a microfluidic biochip, a sample liquid driving device, and a seal docking structure according to one embodiment of the present invention;

fig. 8 is a schematic enlarged view of a portion a in fig. 7;

FIG. 9 is an enlarged view of a portion of the structure of a microfluidic biochip and a sealing and docking mechanism according to a further embodiment of the present invention;

FIG. 10 is a schematic block diagram of the lifting mechanism and the sample stage in a disassembled state according to one embodiment of the present invention;

fig. 11 is a schematic structural view of a refrigerator according to one embodiment of the present invention;

fig. 12 is a schematic exploded view of a door body according to an embodiment of the present invention.

Detailed Description

The invention firstly provides a micro-fluidic detection system for a refrigerator, which is used for qualitatively or quantitatively detecting preset detection parameters of a sample liquid, wherein the preset detection parameters can be, for example, pesticide residue parameters for indicating whether pesticide residue exceeds the standard and/or specific values of the pesticide residue, nutrient parameters for indicating whether nutrient elements reach the standard and/or specific contents of the nutrient elements, specific substance parameters for indicating whether specific harmful substances (such as specific viruses) exceed the standard and/or the specific contents, and the like.

Fig. 1 is a schematic structural view of a microfluidic detection system for a refrigerator according to an embodiment of the present invention, fig. 2 is a schematic structural exploded view of the microfluidic detection system for a refrigerator according to an embodiment of the present invention, fig. 3 is a schematic structural view of an internal structure of the microfluidic detection system according to an embodiment of the present invention, and fig. 4 is a schematic structural exploded view of the internal structure of the microfluidic detection system according to an embodiment of the present invention. For ease of understanding, a sample cup 2 is also shown in fig. 1-4.

Referring to fig. 1 to 4, a microfluidic detection system 1 according to the present invention includes a microfluidic biochip 10, a sample liquid driving device 40, a sealing and docking structure 90, and a detection mechanism 20. It will be appreciated by those skilled in the art that the specific selection of the microfluidic biochip 10 and detection mechanism 20 used in the microfluidic detection system may vary when the predetermined detection parameters for detection are different. For example, when the microfluidic detection system is used for pesticide residue detection, the microfluidic biochip 10 can be a microfluidic pesticide residue detection chip capable of providing detection conditions for pesticide residue, and the detection mechanism 20 can be a pesticide residue detection mechanism capable of detecting pesticide residue parameters of pesticide residue.

Fig. 5 is a schematic structural view of a microfluidic biochip according to an embodiment of the present invention, and referring to fig. 5, the microfluidic biochip 10 has a sample inlet 111, a communication port 112, and a detection cell 121 formed therein, and the sample inlet 111, the detection cell 121, and the communication port 112 are sequentially communicated through a microchannel 14, thereby forming a main channel. The micro flow channel of the present invention means a micro flow channel or a capillary flow channel having a flow area within a predetermined size range so as to have a proper ability to hold liquid therein. The sample inlet 111 and the communication port 112 may be formed at the end of the microfluidic biochip 10. Further, the sample inlet 111 and the communication port 112 are preferably formed at different ends of the microfluidic biochip 10.

The sample liquid driving device 40 is in communication with the communication port 112, and is configured to cause the sample liquid in contact with the sample inlet 111 to enter the micro flow channel and flow to the detection cell 121 through the micro flow channel. Specifically, the sample liquid driving device 40 may form a negative pressure in the main channel by sucking air outward, so as to allow the sample liquid contacting the sample inlet 111 to flow into the microchannel 14 under the negative pressure.

The hermetic docking mechanism 90 is used to form a fluid tight connection between the microfluidic biochip 10 and the sample liquid driving device 40.

The detection mechanism 20 is used for detecting the detection cell 121 to obtain preset detection parameters of the sample liquid. Specifically, the detection cell 121 may be provided with a detection reagent in advance, or the detection reagent may be manually or automatically added to the detection cell 121, so that the detection mechanism 20 detects the detection cell 121 after the sample solution in the detection cell 121 reacts with the detection reagent therein.

In a specific embodiment, when the detecting mechanism 20 is a pesticide residue detecting mechanism for detecting pesticide residue parameters of pesticide residue, an enzyme inhibition rate method can be used to perform rapid qualitative detection on whether pesticide residue in the sample liquid exceeds standard. At this time, the microfluidic biochip 10 further includes a reaction cell 122 formed therein, and the reaction cell 122 is located on a main channel formed by sequentially communicating the sample inlet 111, the detection cell 121, and the communication port 112, and is communicated between the sample inlet 111 and the detection cell 121, so that the sample solution firstly reacts with the reaction reagent in the reaction cell 122 and then flows into the detection cell 121. The reaction cell 122 is communicated with the sample inlet 111, and the reaction cell 122 is communicated with the detection cell 121 through the microchannel 14. The reaction reagent and the detection reagent for pesticide residue detection can be an enzyme reagent and a color-developing agent respectively. The reaction cell 122 is used for allowing the sample liquid to react with the enzyme reagent therein, and the sample liquid after reacting with the enzyme reagent flows into the detection cell 121 to react with the color developing agent in the detection cell 121. The detection mechanism 20 may be selected as a photoelectric detection mechanism, and may include a light source 21 and a photosensitive element 22 respectively disposed at two opposite sides of the microfluidic biochip 10 and facing the detection cell 121, light emitted from the light source 21 is irradiated to the detection cell 121, and light passing through the detection cell 121 is guided to the photosensitive element 22, so that it is beneficial to determine absorbance change in the detection cell 121 through a light intensity signal received by the photosensitive element 22, and further calculate the pesticide residue inhibition rate. Further, the detecting mechanism 20 further includes a heating plate 24 for supplying heat to the detecting cell 121 and a temperature controller 25 for controlling the heating plate 24 to have a constant heating power, so that the sample liquid and the detecting reagent in the detecting cell 121 can be reacted sufficiently and quickly.

The micro-fluidic detection system 1 of the present invention is particularly provided with a sealing and docking mechanism 90 between the micro-fluidic biochip 10 and the sample liquid driving device 40, which is used for realizing fluid sealing connection between the two, and avoiding the problems of air leakage, liquid leakage, etc. at the connection between the two to affect the pressure in the main channel formed by the sequential communication of the sample inlet 111, the detection cell 121 and the communication port 112, thereby further affecting the sample liquid entering the main channel, ensuring the normal sample introduction effect of the micro-fluidic detection system 1, and facilitating the accurate control of sample introduction by the sample liquid driving device 40.

Fig. 6 is a schematic exploded view of a microfluidic biochip, a sample liquid driving apparatus, and a sealing and docking structure according to an embodiment of the present invention, fig. 7 is a schematic cross-sectional view of the microfluidic biochip, the sample liquid driving apparatus, and the sealing and docking structure according to an embodiment of the present invention, and fig. 8 is a schematic enlarged view of a portion a in fig. 7. In some embodiments, the seal interface mechanism 90 may include a seal connection 91 and a resilient press 92. The sealing connector 91 is connected between the microfluidic biochip 10 and the sample liquid driving device 40, and a connection channel penetrating the sealing connector 91 is formed therein. The elastic pressing member 92 is used for applying an elastic force to the sealing connector 91 so that the sealing connector 91 is simultaneously in sealing abutment with the sample liquid driving device 40 and the microfluidic biochip 10, thereby allowing the communication ports 112 of the sample liquid driving device 40 and the microfluidic biochip 10 to be in sealing communication through the connection channels inside the sealing connector 91.

According to the application, the sealing and butting mechanism 90 is further designed to comprise the sealing connector 91 and the elastic pressing piece 92, and elastic acting force can be applied to the sealing connector 91 through the elastic pressing piece 92, so that the sealing connector 91 is always kept in a state of being tightly and hermetically butted with the sample liquid driving device 40 and the microfluidic biochip 10, the problems of looseness, fracture and the like caused by long-time use due to the adoption of other butting mechanisms are avoided, the long-term and reliable fluid sealing and communicating relation between the sample liquid driving device 40 and the communicating port 112 of the microfluidic biochip 10 is ensured, and the sealing effect between the two is improved.

In some embodiments, an elastic pressing member 92 is disposed between the sealing connector 91 and the sample liquid driving device 40 to apply an elastic force to the sealing connector 91 toward the microfluidic biochip 10, thereby urging the sealing connector 91 to be tightly and elastically abutted against the microfluidic biochip 10 by the elastic force and urging the sealing connector 91 to be elastically and sealingly abutted against the sample liquid driving device 40 by a reaction force of the microfluidic biochip 10 against the sealing connector 91. It is understood that in some alternative embodiments, the elastic pressing member 92 may be disposed between the sealing connector 91 and the microfluidic biochip 10, and the operation principle is the same as that of the above embodiments, and thus the description is omitted.

In some embodiments, the sample fluid drive device 40 communicates with the communication port 112 through the connecting line 46. The communication port 112 may be formed on the top of the microfluidic biochip 10, and the sample liquid driving device 40 may be adjacently disposed on the lateral side of the microfluidic biochip 10 to avoid the sample liquid driving device 40 from being adversely affected by the leakage liquid that may be generated by the microfluidic biochip 10. The connecting line 46 may communicate with the top of the sample liquid driving device 40 to bridge between the sample liquid driving device 40 and the microfluidic biochip 10.

Further, the seal connection 91 includes a first connection post 911 convexly extending toward the connection pipe 46, and a connection passage inside the seal connection 91 penetrates the first connection post 911. The first connection post 911 is inserted into the connection tube 46 and is brought into close contact with the inner wall of the connection tube 46, so that the connection channel inside the sealing connector 91 is in sealed communication with the connection tube 46, and thus the connection channel inside the sealing connector 91 is in sealed communication with the sample liquid driving device 40. That is, the first connecting post 911 and the connecting pipeline 46 are sleeved together, and the sleeved connection manner can enlarge the contact surface area between the two, thereby improving the tightness of the connection between the two.

In some embodiments, the elastic pressing member 92 may be a spring, one end of the spring abuts against a fixedly disposed end plate 513, and the other end abuts against the sealing connector 91, and the end plate 513 and the microfluidic biochip 10 are respectively located at two opposite sides of the sealing connector 91. In particular, in the mounted state of the microfluidic biochip 10, the springs are in a compressed state, thereby generating an elastic force for urging the sealing connection 91 to have a tendency to move towards the microfluidic biochip 10. The number of the elastic pressing members 92 can be two or more than two, so as to increase the magnitude of the elastic acting force acting on the microfluidic biochip 10, make the elastic acting force applied on the microfluidic biochip 10 more balanced, avoid the inclination, and further improve the effect of sealing connection.

Further, the sealing and docking mechanism 90 further includes a guide rod 93, and the spring is sleeved on the guide rod 93 to prevent the spring from displacing. One end of the guide rod 93 is fixedly connected with the sealing connector 91, and the other end of the guide rod is in contact with a hall switch 94 after the microfluidic biochip 10 is in sealing butt joint with the sealing connector 91, so that the hall switch 94 is prompted to generate a trigger signal for indicating that the microfluidic biochip 10 is installed in place, a user is prompted, structural damage caused by excessive installation of the microfluidic biochip 10 is avoided, and the use experience of the user is improved.

Specifically, the guide rods 93 may pass through the end plate 513 and be retained and supported by the end plate 513. When the microfluidic biochip 10 is mounted upward in the vertical direction, the head of the guide rod 93 above the end plate 513 may be provided with an expanded diameter portion, and when the microfluidic biochip 10 is not mounted, the spring is in a natural state, and the guide rod 93 and the sealing connector 91 move downward under the action of its own gravity until the expanded diameter portion of the guide rod 93 abuts against the end plate 513, thereby supporting the guide rod 93 and the sealing connector 91.

In some embodiments, the sealing connector 91 further comprises a first connection block 912 for directly interfacing with the microfluidic biochip 10 and a second connection block 913 disposed at a side of the first connection block 912 facing away from the microfluidic biochip 10, the first connection column 911 is extended from the second connection block 913 to protrude toward the connection line 46. A column hole is formed inside the second connection block 913, a second connection column 914 is formed on the side of the first connection block 912 facing away from the microfluidic biochip 10, the second connection column 914 is plugged in the column hole 9131, and a connection channel inside the sealing connector 91 penetrates through the first connection column 911, the first connection block 912 and the second connection block 913. Therefore, the first connection block 912 and the second connection block 913 form a sleeved connection relationship, so that the contact surface area between the two is enlarged, and the connection tightness between the two is improved.

FIG. 9 is an enlarged view of a portion of the structure of a microfluidic biochip and a sealing and docking mechanism according to a further embodiment of the present invention. Further, a trumpet-shaped tapered groove 9121 which is gradually expanded from inside to outside along the axial direction is formed at the end part of the first connecting block 912, which is adjacent to the microfluidic biochip 10, and the end part of the microfluidic biochip 10 where the communication port 112 is located is a tapered protrusion 15 which is gradually tapered towards the direction where the communication port 112 is located along the axial direction, so that when the microfluidic biochip 10 is in sealed butt joint with the sealing and butting mechanism 90, the tapered protrusion 15 is inserted into the trumpet-shaped tapered groove 9121 of the first connecting block 912, and thus the microfluidic biochip 10 is in sealed butt joint with the sealing and butting mechanism 90 in a sleeved mode, the contact surface area between the two is enlarged, and the sealing performance of connection between the two is improved.

In some embodiments, the microfluidic detection system 1 further comprises a clamping mechanism 51, and the clamping mechanism 51 is configured to clamp the microfluidic biochip 10 to maintain a fluid-tight connection with the sample liquid driving device 40. The direction of the acting force generated by the clamping mechanism 51 on the microfluidic biochip 10 is perpendicular to the direction of the acting force generated by the sealing and docking mechanism 90 on the microfluidic biochip 10. Thereby fixing and supporting the microfluidic biochip in two different directions, respectively, and improving the stability of the mounting of the microfluidic biochip 10.

Further, the clamping mechanism 51 may comprise two elastic clamping jaws 511 arranged oppositely to apply opposing forces to the microfluidic biochip 10 clamped between the two elastic clamping jaws 511.

In some embodiments, the microfluidic detection system 1 further comprises a cantilever key 52 suspended at one side of the microfluidic biochip 10, the cantilever key 52 simultaneously abutting against oppositely disposed inner sides of the two elastic clamping jaws 511 to apply an outward force to the inner sides of the two elastic clamping jaws 511 when the cantilever key 52 is subjected to a force towards the microfluidic biochip 10, thereby elastically deforming the two elastic clamping jaws 511 towards outer directions away from each other. That is, when the microfluidic biochip 10 needs to be disassembled, the user only needs to press the cantilever button to release the clamping effect of the two elastic clamping jaws 511 on the microfluidic biochip 10, so as to release the microfluidic biochip 10, the operation is very simple and convenient, and the cantilever button 52 has a very simple structure and a very smart design.

In some embodiments, the sample liquid driving device 40 may be a micro syringe pump, which may form a negative pressure in the main channel by pumping air outwards, so that the sample liquid contacting with the sample inlet 111 enters the main channel under the action of the negative pressure. Specifically, the sample liquid driving device 40 includes a driving motor 41, a vertically extending syringe 42, a lead screw 43, a slider 44, and a piston 45.

The syringe 42 is fixed on the holder 87, and the top of the syringe 42 is in sealed communication with the communication port 112 on the top of the microfluidic biochip 10 through the connecting line 46. The lead screw 43 extends vertically and is connected to the driving motor 41 to be rotated by the driving motor 41. The slider 44 is inserted into the screw rod 43 and is connected with the screw rod 43 by screw threads so as to move up and down along the screw rod 43 along with the rotation of the screw rod 43. Specifically, the bracket 87 may be formed with a guide groove extending in the vertical direction, in which the slider 44 is located, to guide the movement of the slider 44 in the up-down direction through the guide groove. The piston 45 is disposed inside the injector 42 and fixedly connected to the slider 44 to move in the vertical direction under the driving of the slider 44, so that when the piston moves in the vertical direction, a negative pressure is generated in the main channel to force the sample liquid contacting the sample inlet 111 to flow into the microchannel and flow into the detection cell 121 through the microchannel, and when the piston moves in the vertical direction, the sample liquid in the main channel is forced to flow toward the sample inlet 111.

In some embodiments, the microfluidic detection system 1 further comprises a sample stage 70, the sample stage 70 is disposed below the microfluidic biochip 10 for placing the sample cup 2, and the sample cup 2 is used for containing the sample liquid. And, the sample stage 70 is arranged to be controllably or operatively moved to transport the sample cup 2 placed thereon by the sample stage 70 to a position allowing the sample liquid in the sample cup 2 to contact the sample inlet 111 of the microfluidic biochip 10. Thus, sample application of the microfluidic biochip 10 is achieved. The user only needs to place the sample cup 2 on the sample stage 70, or place the sample cup 2 on the sample stage 70 and then move the sample stage 70 to the position contacting with the sample inlet 111 of the microfluidic biochip 10, so that the sample adding operation is very convenient and fast, and time and labor are saved. In addition, the sample stage 70 is movably arranged, so that complex structures such as a sample liquid delivery pump, a delivery pipeline, a sampling needle and the like are omitted, the structure of the microfluidic detection system 1 is very simple, and the microfluidic detection system is suitable for being integrated on a refrigerator and is convenient for family use.

Further, the microfluidic detection system 1 further includes a lifting mechanism 60 for driving the sample stage 70 to move up and down, so that the sample stage 70 is switched between a detection position allowing the sample liquid in the sample cup 2 placed on the sample stage 70 to contact the sample inlet 111 and an initial position at a preset distance below the detection position. That is, the sample stage 70 may be automatically lifted and lowered by the upgrade mechanism 60.

Fig. 10 is a schematic configuration diagram of the elevating mechanism and the sample stage in a disassembled state according to one embodiment of the present invention. In some embodiments, the lift mechanism 60 may include a lift motor 61, a drive screw 62, and a nut 63. The lift motor 61 is used to output a driving force. The driving screw 62 is disposed in a vertical direction and connected to an output shaft of the elevating motor 61 to be rotated by the elevating motor 61. The nut 63 is arranged on the transmission screw rod 62 in a penetrating way and is in threaded connection with the transmission screw rod 62 so as to move up and down along the transmission screw rod 62 along with the rotation of the transmission screw rod 62. The sample stage 70 is fixedly connected with the nut 63 so as to drive the sample stage 70 to move up and down through the nut 63.

Further, the lifting mechanism 60 further includes a slide rail 64 and a slider 65. The slide rail 64 is arranged beside the transmission screw rod 62 in parallel with the transmission screw rod 62, the slide block 65 is movably arranged on the slide rail 64, and the sample table 70 is fixedly connected with the slide block 65 so as to guide the sample table 70 to move up and down through the matching of the slide rail 64 and the slide block 65. Specifically, the slide block 65 is driven to move synchronously when the sample stage 70 moves in the up-down direction under the action of the driving module, the slide block 65 is limited on the slide rail 64, and the slide rail 64 has guiding and limiting functions on the movement of the slide block 65, so that the sample stage 70 is indirectly guided and limited, the sample stage 70 is prevented from being shifted or stuck in the moving process, and the moving stability of the sample stage 70 is improved. Specifically, the sample stage 70 may include a horizontal connecting plate 74 penetrating the driving screw 62 and fixedly connected to the nut 63, and a vertical connecting plate 75 extending upward perpendicular to the horizontal connecting plate 74, the vertical connecting plate 75 being fixedly connected to the slide block 65.

In some embodiments, the lifting mechanism 60 further comprises a limit switch 66, and the limit switch 66 is disposed adjacent to the upper portion of the transmission screw 62 to cause the lifting motor 61 to stop operating when the sample stage 70 moves upward to touch the limit switch 66. And, the position of the limit switch 66 is set so that the sample stage 70 is in its detection position when the elevating motor 61 stops operating under the trigger of the limit switch 66. The elevating motor 61 is not operated to keep the sample stage 70 at its detecting position. The detection position of the sample table 70 is positioned through the limit switch 66, the positioning is accurate, and the problem that the sample table 70 exceeds the detection position to continuously move to cause the damage of the sample table 70, the microfluidic biochip 10 and other structures can be avoided.

In some embodiments, the sample stage 70 may include a support stage 71 and an oscillator 72. The support stage 71 is for supporting the sample cup 2. Specifically, the supporting platform 71 may be a horizontally disposed supporting plate, and a groove for placing the bottom of the sample cup 2 therein may be disposed on the supporting plate, so as to prevent the sample cup 2 from toppling or shaking during the moving process of the sample platform 70, thereby improving the stability of placing the sample cup 2. The support table 71 is fixedly connected with the horizontal connecting plate 74.

The oscillator 72 is disposed on the supporting platform 71, and is configured to oscillate the sample cup 2 after the sample cup 2 is placed on the supporting platform 71, so that a buffer solution in the sample cup 2 and a sample are sufficiently mixed to generate a sample solution, and a substance to be detected on the sample is sufficiently dissolved in the buffer solution to obtain the sample solution with a suitable concentration. The buffer solution may be pre-set in the sample cup 2 by means of manual addition, or may be automatically transferred to the sample cup 2 by the driving means after the sample cup 2 is placed on the sample stage 70.

In some embodiments, the sample stage 70 further comprises a load cell 73, the load cell 73 being arranged below the support stage 71 for weighing the weight of the sample in the sample cup 2, thereby allowing the buffer drive 30 to deliver a preset amount of buffer matching the weight of the sample to the sample cup 2. Usually, the sample is taken at will by the home user, for example, a small piece of vegetable leaves is torn off at will, and therefore, in order to ensure the accuracy of the measurement result, the amount of the buffer solution input into the sample cup 2 needs to be matched with the amount of the sample, so as to generate the sample solution with proper concentration. The weight of the sample can be automatically and accurately obtained through the weighing sensor 73 arranged below the supporting table 71, so that the buffer solution driving device 30 is automatically controlled to input the matched amount of buffer solution into the sample cup 2, the accuracy of the measuring result is ensured, various problems of inconvenience in use, complex operation, large error and the like caused by manual measurement of the sample by a user are avoided, and the automation degree of the microfluidic detection system and the use experience of the user are further improved.

It should be noted that, in some alternative embodiments, the sample stage 70 may be fixed, and the microfluidic pesticide residue detection chip 10 may be configured to be movable, which can also facilitate the sampling operation.

In some embodiments, the microfluidic detection system 1 further comprises a housing 80. The housing 80 has a console 83 formed thereon and opened toward the front side thereof, and the sample stage 70 is at least partially located in the console 83, thereby facilitating the user to perform operations of placing the sample cup 2, taking out the sample cup 2, and the like in the console 83. A drip tray 88 may be disposed below the console 83 to receive liquid that may drip from the console 83 to prevent contamination of the console 83. At least some of the sections of the microfluidic biochip 10, the detection mechanism 20, the buffer solution bottle 36, and the buffer driving device 30 are disposed in the housing 80. Further, the housing 80 is provided with a first structural connection 81 for connecting with a cabinet or door of the refrigerator, and a first electrical connection 82 for forming an electrical connection between the microfluidic detection system 1 and an electrical control device of the refrigerator 100, so as to allow the microfluidic detection system 1 to be mounted to the cabinet or door of the refrigerator as a whole.

In some embodiments, the microfluidic detection system 1 further comprises a buffer solution bottle 36 and a buffer solution driving device 30. The buffer bottle 36 is disposed in the housing 80 and is used for containing a buffer. The buffer driving device 30 is disposed in the housing 80 and is in communication with the buffer bottle 36 to controllably drive the buffer in the buffer bottle 36 into the sample cup 2 placed on the sample stage 70, so that the buffer is mixed with the sample in the sample cup 2 to generate the sample solution. Specifically, the buffer solution bottle 36 is communicated with the buffer solution driving device 30 through the inlet tube 32. The extraction tube 31 of the buffer driving device 30 extends to the sample stage 70. The method mainly aims at that a detected sample is a solid sample, and a buffer solution is needed to dissolve a substance to be detected on the solid sample into the solid sample so as to form a sample solution; alternatively, the sample is a liquid sample, but the concentration is too high, and it is necessary to dilute the sample with a buffer solution to produce a sample solution. For example, in the case of pesticide residue detection, the sample to be detected is usually a solid food residue such as epidermis or leaf, and the sample is placed in a buffer solution, and the pesticide residue on the sample is dissolved in the buffer solution to form a sample solution.

In particular, the buffer drive 30 may be a peristaltic pump, a diaphragm pump, or other suitable type of drive. The peristaltic or membrane pump generates a large vibration in its radial direction when it is operated, and in order to avoid that the vibration is transmitted to the microfluidic biochip 10, the radial outer side of the peristaltic or membrane pump may be provided with an elastic vibration reduction member 35. The elastic damping member 35 may be fitted over the outside of the buffer driving device 30 and supported in the housing 80 by the clamping action of the bracket 87 and the fixing block 89, and the fixing block 89 may be fixed to the support plate 86.

In some embodiments, the microfluidic detection system 1 further comprises a circuit board 53, a display device 56, and a switch button 57, the circuit board 53 being disposed within the housing 80 and electrically connected to the first electrical connector 82 on the housing 80. The electrical components (e.g., the lifting mechanism 60, the buffer driving device 30, the sample liquid driving device 40, the display device 56, the switch button 57, etc.) of the microfluidic detection system 1 are all directly or indirectly electrically connected to the circuit board 53. The display device 56 is disposed on the front side of the housing 80 and electrically connected to the circuit board 53 for displaying the detection result of the detection mechanism 20. The switch button 57 is disposed on the front side of the housing 80 and electrically connected to the circuit board 53 for activating and/or deactivating the detection function of the microfluidic detection system 1. That is, the user can activate, suspend, or deactivate the detection function of the microfluidic detection system 1 by operating the switch button 57.

In some embodiments, the housing 80 may include a rear case 84 at a rear side and a front panel 85 connected to a front side of the rear case 84. The rear housing 84 and the front panel 85 define a receiving cavity therebetween when assembled. Further, a support plate 86 and a bracket 87 are provided in the housing chamber of the housing 80. The supporting plate 86 is fixedly connected to the rear housing 84, and at least a part of the elevating mechanism 60 (e.g., an immovable part of the elevating mechanism) and the buffer driving unit 30 are fixed to the supporting plate 86. The holder 87 is fixedly attached to the front side of the support plate 86, and both the microfluidic biochip 10 and the sample liquid driving device 40 are directly or indirectly supported on the holder 87. Thus, the elevating mechanism 60, the buffer driving device 30, the microfluidic biochip 10, and the sample liquid driving device 40 can be stably supported by the support plate 86 and the holder 87 in the accommodation chamber formed between the rear case 84 and the front panel 85.

In some embodiments, the lifting mechanism 60 may be disposed at a lateral side of the sample stage 70, the buffer driving device 30 may be disposed at one lateral side of the microfluidic biochip 10 and above the lifting mechanism 60, the sample liquid driving device 40 is disposed at the other lateral side of the microfluidic biochip 10, and the buffer bottle 36 is disposed at a side of the sample liquid driving device 40 facing away from the microfluidic biochip 10. The microfluidic biochip 10, the sample stage 70, the lifting mechanism 60, the buffer driving device 30, the sample liquid driving device 40 and the buffer liquid bottle 36 which are arranged in this way fully utilize the dimensional characteristics of each module in the vertical direction and the transverse direction, so that the arrangement of each module is more compact, and the occupied space is reduced as much as possible. Moreover, the modules are arranged side by side only in the vertical direction and the transverse direction, so that the thickness of the microfluidic detection system 1 in the front and rear directions is reduced as much as possible, and the microfluidic detection system is more suitable for being integrated on a refrigerator.

Further, a partition 861 extending transversely may be disposed between the buffer driving device 30 and the lifting mechanism 60 to prevent the buffer driving device 30 from leaking liquid and dropping on the lifting mechanism 60, which may affect the normal operation of the lifting mechanism 60. The partition 861 may be fixed to the support plate 86.

The present invention also provides a refrigerator, and fig. 11 is a schematic structural view of a refrigerator according to an embodiment of the present invention. The refrigerator 100 of the present invention includes the microfluidic detection system 1 according to any of the above embodiments to integrate the microfluidic detection system 1 on the refrigerator 100. The refrigerator 100 is frequently used in daily life, and the refrigerator 100 is mainly used for storing food materials, so that when the microfluidic detection system 1 is integrated on the refrigerator 100, a user can conveniently perform a detection operation of a food material sample by using the microfluidic detection system 1.

Further, the refrigerator 100 further includes a cabinet 200 and a door 300, wherein the cabinet 200 defines a storage space therein, and the door 300 is connected to the cabinet 200 and is used for opening and/or closing the storage space. The micro-fluidic detection system 1 is preferably arranged on the door 300, so that the operation is convenient, the original storage space in the refrigerator body 200 cannot be occupied, and the storage capacity of the refrigerator 100 cannot be influenced.

Fig. 12 is a schematic exploded view of a door body according to an embodiment of the present invention. In some embodiments, the hollow window 301 is disposed on the front side of the door 300, and the sample stage 70 of the microfluidic detection system 1 is exposed to the front side of the door 300 through the hollow window 301, so that a user can place a sample cup on the sample stage 70 without opening the door 300, the problem of serious cold leakage caused by opening the door 300 during each detection is avoided, the heat preservation performance of the refrigerator 100 is ensured, and energy consumption is reduced.

Specifically, the door body 300 may include a panel 302 for forming a front portion thereof, a door liner 303 for forming a rear portion thereof, and a foam insulation layer (not shown) disposed between the panel 302 and the door liner 303, and the cutout window 301 is opened on the panel 302. An embedded box 304 is embedded between the panel 302 and the door liner 303 before the foaming heat-insulating layer is formed, and the microfluidic detection system 1 is arranged in the embedded box 304. That is, the pre-embedded box 304 is pre-arranged between the panel 302 and the door liner 303 before the door 300 is foamed, so as to reserve a space for installing the microfluidic detection system 1 between the panel 302 and the door liner 303.

Further, the embedded box 304 is attached to the rear surface of the panel 302, the front side of the embedded box 304 is open and faces the hollow window 301, so that the micro-fluidic detection system 1 is allowed to be installed into the embedded box 304 from front to back through the hollow window 301, and the installation convenience of the micro-fluidic detection system 1 is improved.

Specifically, the embedded box 304 may be provided with a second structure connector 305 connected to the first structure connector 81 in a matching manner and a second electrical connector 306 electrically connected to the first electrical connector 82, and the second electrical connector 306 is electrically connected to the electric control device of the refrigerator 100. Therefore, the micro-fluidic detection system 1 is integrally mounted on the door 300 by arranging corresponding structural connecting pieces and electrical connecting pieces on the embedded box 304 and the shell 80, so that the connection between the whole micro-fluidic detection system 1 and the refrigerator 100 is realized in both structural and circuit aspects. Therefore, the assembly process of the microfluidic detection system 1 is simplified, and the disassembly or the maintenance of the microfluidic detection system 1 is facilitated.

The refrigerator 100 of the present application is a refrigerator in a broad sense, and includes not only a so-called refrigerator in a narrow sense but also a storage device having a refrigerating, freezing or other storage function, for example, a refrigerator, a freezer, and the like.

It should be further understood by those skilled in the art that the terms "upper", "lower", "front", "back", "top", "bottom", etc. used in the embodiments of the present invention are used as terms for indicating the orientation or positional relationship with respect to the actual use state of the microfluidic detection system 1 and the refrigerator 100, and these terms are only used for convenience of describing and understanding the technical solution of the present invention, and do not indicate or imply that the device referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

Thus, it should be appreciated by those skilled in the art that while a number of exemplary embodiments of the invention have been illustrated and described in detail herein, many other variations or modifications consistent with the principles of the invention may be directly determined or derived from the disclosure of the present invention without departing from the spirit and scope of the invention. Accordingly, the scope of the invention should be understood and interpreted to cover all such other variations or modifications.

Claims

1. A microfluidic detection system for a refrigerator, comprising:

2. The microfluidic detection system of claim 1, wherein the hermetic docking mechanism comprises:

3. The microfluidic detection system of claim 2,

the elastic pressing piece is arranged between the sealing connecting piece and the sample liquid driving device to apply an elastic acting force towards the microfluidic biochip to the sealing connecting piece, so that the sealing connecting piece is tightly and elastically abutted against the microfluidic biochip under the action of the elastic acting force, and the sealing connecting piece is elastically and hermetically abutted against the sample liquid driving device under the action of the reaction force of the microfluidic biochip on the sealing connecting piece.

4. The microfluidic detection system of claim 3,

the sample liquid driving device is communicated with the communication port through a connecting pipeline;

5. The microfluidic detection system of claim 3,

the elastic pressing piece comprises a spring, one end of the spring abuts against a fixedly arranged end plate, the other end of the spring abuts against the sealing connecting piece, and the end plate and the microfluidic biochip are respectively positioned on two opposite sides of the sealing connecting piece.

6. The microfluidic detection system of claim 5,

the sealing butt joint structure further comprises a guide rod, and the spring is sleeved on the guide rod; and is

7. The microfluidic detection system of claim 4,

the sealing connecting piece also comprises a first connecting block used for being directly butted with the microfluidic biochip and a second connecting block arranged on the side, away from the microfluidic biochip, of the first connecting block, and the first connecting column protrudes and extends from the second connecting block to the connecting pipeline;

8. The microfluidic detection system of claim 1, further comprising:

9. The microfluidic detection system of claim 8,

the clamping mechanism comprises two elastic clamping jaws which are oppositely arranged so as to apply opposite acting force to the microfluidic biochip clamped between the two elastic clamping jaws; and is

10. The microfluidic detection system of claim 1, further comprising:

The sample stage comprises a supporting table for supporting a sample cup and an oscillator arranged on the supporting table, wherein the oscillator is used for oscillating the sample cup after the sample cup is placed on the supporting table, so that the buffer solution and the sample in the sample cup are fully mixed to generate the sample solution.

11. A refrigerator comprising a microfluidic detection system according to any one of claims 1 to 10.