CN114306567A - Traditional Chinese medicine oral liquid for preventing and treating chicken glandular myositis - Google Patents

Abstract

The invention discloses a traditional Chinese medicine oral liquid for preventing and treating chicken adenofibromyositis, which is a traditional Chinese medicine compound prepared by scientifically matching 6 traditional Chinese medicines including salvia miltiorrhiza, codonopsis pilosula, baked ginger, vinegar rhizoma cyperi, fried bighead atractylodes rhizome and liquorice according to the dialectical analysis of traditional Chinese veterinary medicines for treating chicken adenofibromyositis, and the traditional Chinese medicine oral liquid has a simple formula, has a remarkable treatment effect on chicken with adenofibromyositis, can improve the growth rate of the chicken, promote the secretion of pepsinogen and alpha-amylase, accelerate the elimination of adenofibromyositis, promote the development of spleen and bursa of fabricius, and improve the immunity.

Description

Traditional Chinese medicine oral liquid for preventing and treating chicken glandular myositis

Technical Field

The invention relates to a traditional Chinese medicine oral liquid for preventing and treating proventriculitis (proventriculitis and muscular gastritis) of chicken, belonging to the technical field of traditional Chinese veterinary medicines.

Background

The chicken adenomyositis is a multi-cause digestive system disorder syndrome which troubles the broiler industry for a long time, is mostly generated in chicks of 2-4 weeks, and mainly shows that cocks, meat beards and toe claws are pale, excrement is thin or is yellow in soil and mixed with feed residues, growth retardation, dyspepsia and low feed reward are caused, adenogastric swelling is like globus and adenogastric mucosa ulcer bleeding, flat and fuzzy nipple, sarcophagous cuticle flavochrome decline, erosion, ulcer, even sub-cuticular ulcer, bleeding and the like. At present, no specific prevention and treatment medicine exists.

Common anti-inflammatory drugs for the chicken adenofibromyositis comprise amoxicillin, clindamycin and the like, gastric acid secretion inhibitors atropine, farnesidine and the like, mycotoxin adsorbents such as montmorillonite powder, hydrated sodium calcium aluminosilicate and the like, and traditional Chinese medicines comprise five-flavor spleen tonifying mixture, sinistra powder and the like. The medicine is used for preventing and treating by protecting gastrointestinal mucosa, promoting gastrointestinal peristalsis, aiding digestion and the like, but has poor effect and is easy to repeat.

The applicant previously developed a method for rapidly constructing a chicken adenofibromyositis model (patent application No. 2021100733568), which is to directly add rhubarb and acetic acid or polyethylene glycol-400 to drinking water of chicken to construct the chicken adenofibromyositis model. The model constructed by the method has obvious clinical symptoms and typical autopsy characteristics, and is suitable for the research of medicaments for preventing and treating the chicken adenoid myositis. On the basis, the applicant further develops the traditional Chinese medicine oral liquid for preventing and treating the chicken adenofibromyositis.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine oral liquid for preventing and treating chicken adenofibromyositis, which is a traditional Chinese medicine compound prepared by scientifically matching 6 traditional Chinese medicines of salvia miltiorrhiza, codonopsis pilosula, baked ginger, vinegar rhizoma cyperi, fried bighead atractylodes rhizome and liquorice according to the syndrome differentiation analysis of traditional Chinese veterinary medicines for treating chicken adenofibromyositis, adopts the treatment principle of warming middle-jiao to dispel cold, and tonifying qi and spleen, and is simple in formula and has a remarkable treatment effect on chicken with adenofibromyositis.

The technical scheme of the invention is as follows: the traditional Chinese medicine oral liquid for preventing and treating chicken glandular myositis is characterized by being prepared from the following raw material medicines in parts by weight: 5-15 parts of salvia miltiorrhiza, 10-20 parts of codonopsis pilosula, 15-20 parts of baked ginger, 3-10 parts of vinegar rhizoma cyperi, 5-20 parts of fried bighead atractylodes rhizome and 10-15 parts of liquorice.

The preferable mixture ratio is (parts by weight): 15 portions of salvia miltiorrhiza, 20 portions of codonopsis pilosula, 20 portions of baked ginger, 10 portions of rhizoma cyperi with vinegar, 20 portions of fried rhizoma atractylodis macrocephalae and 15 portions of liquorice.

The production process comprises the following steps: weighing the raw materials according to the weight parts, decocting the raw materials in water for 2-3 times, concentrating the decoction until the ratio of the raw materials is 1: 0.5-1.5, and adding a preservative to obtain the traditional Chinese medicine.

The method comprises the following specific steps:

(1) weighing: weighing the raw material medicines according to the parts by weight;

(2) soaking and decocting: the 6 medicines are decocted twice by adding water, 5 to 10 times of water by mass is added for the first time, the mixture is soaked for 2 to 3 hours, boiled for 0.5 to 1.5 hours and filtered; adding 3-5 times of water by mass for the second time, boiling for 0.1-1.0 h, and filtering; mixing the filtrates for 2 times;

(3) concentration: concentrating to obtain medicinal liquid with a crude drug ratio of 1: 0.8-1.2, standing, and filtering;

(4) corrosion prevention and constant volume: adding sodium benzoate into the filtrate for corrosion prevention;

(5) filling: and (6) filling and sealing.

The medicinal properties and pharmacological analysis of the selected Chinese herbal medicines are as follows:

1, vinegar rhizoma cyperi: is dried rhizome of Cyperusrotundus L. of Cyperaceae. Pungent, slightly bitter, slightly sweet and neutral. It enters liver, spleen and triple energizer meridians. Has the effects of promoting qi circulation, resolving stagnation, regulating menstruation and relieving pain. Vinegar processed rhizoma Cyperi has effects of activating qi-flowing, relieving epigastric distention, dispersing stagnated liver qi, resolving stagnation, regulating menstruation, relieving pain, preventing miscarriage, and relieving vomit, and can be used for treating stagnation of qi due to liver-qi, distending pain of chest and hypochondrium, abdominal region, dyspepsia, incoordination between liver and stomach, qi stagnation, and stagnation of qi of spleen and stomach.

2, radix codonopsis: is dried root of Codonopsis pilosula Codonopsispirosula (Franch.) Nannf., Codonopsis pilosula Suffruticosa (West Codonopsis pilosula), Codonopsispirosula Nannf., var. modesta (Nannf.), L.T.Shen or Codonopsist angshenoliv. Sweet and neutral. It enters spleen and lung meridians. Has the effects of invigorating spleen and replenishing qi, and invigorating spleen and lung. Can be used for treating spleen and lung deficiency, short breath, palpitation, anorexia, loose stool, asthma, cough, internal heat, and diabetes.

3, salvia miltiorrhiza: is dried root and rhizome of Salvia Miltiorrhiza Bunge of Labiatae. Bitter and slightly cold. It enters heart and liver meridians. Dispel stasis and alleviate pain, activate blood and dredge meridians, clear heart fire and relieve restlessness. Can be used for treating abdominal mass, pain of chest and abdomen, pain due to arthralgia, pyocutaneous disease, swelling and pain, vexation, and insomnia; hepatosplenomegaly, etc.

4, frying the bighead atractylodes rhizome: is dried rhizome of Atractylodes macrocephala Koidz of Compositae. Bitter, sweet and warm. It enters spleen and stomach meridians. Has the effects of strengthening spleen, tonifying qi, eliminating dampness, inducing diuresis, suppressing sweating and preventing miscarriage. Can be used for treating spleen deficiency, anorexia, abdominal distention, diarrhea, phlegm retention, dizziness, palpitation, edema, spontaneous perspiration, and threatened abortion.

5, baked ginger: the product is prepared by parching dried rhizoma Zingiberis of Zingiber officinale Rosc of Zingiberaceae to slight black surface and brown yellow color. Collected in winter, removed fibrous root and silt, dried in the sun or dried at low temperature. Bitter, pungent and warm herbs enter spleen, stomach, kidney, heart and lung meridians. Has effects of warming spleen and stomach, dispelling cold, warming channels and stopping bleeding. Pao Jiang is pungent and dry, and has weak property and mild and lasting action, but it is weaker than gan Jiang in warming the interior, and is good at warming the middle energizer to alleviate pain, checking diarrhea and warming meridians to stop bleeding. Can be used for treating abdominal pain, diarrhea and hemorrhage due to deficiency-cold of middle-warmer energy.

6, liquorice: the product is dried root of Glycyrrhiza uralensis Fisch, Glycyrrhiza inflata Bat, or Glycyrrhiza glabra L. Collected in spring and autumn, removed fibrous root, and dried in the sun. Sweet in flavor and neutral in nature. It enters heart, lung, spleen and stomach meridians. Has the effects of invigorating spleen and replenishing qi, clearing away heat and toxic materials, eliminating phlegm and stopping cough, relieving spasm and pain, and harmonizing the medicines. Can be used for treating weakness of spleen and stomach, asthenia, palpitation, short breath, cough, excessive phlegm, abdominal and limb spasm, pain, carbuncle, swelling, sore, and drug toxicity and strong nature.

The chicken adenofibromyositis is mainly characterized in that the spleen and stomach of a young chicken are not well developed, the young chicken is fed with high-energy and high-protein feed for a long time, the young chicken is lack of necessary activities after intensive high-density feeding, food stagnation can not be realized, dampness and heat are transformed, the spleen is in damp stagnation, qi movement is blocked, blood circulation is not smooth, glandular and gastric edema occurs, excrement is thin, food essence cannot be transformed and distributed to the whole body, spleen qi deficiency and spleen yang deficiency occur for a long time, spleen and stomach deficiency cold is caused, grain indigestion occurs, a feed is discharged, and a middle veterinarian distinguishes a spleen and stomach deficiency cold as a spleen and stomach deficiency cold syndrome.

According to the dialectical analysis of the chicken gland and muscular gastritis by a middle veterinarian, the invention consists of 6 traditional Chinese medicines of salvia miltiorrhiza, codonopsis pilosula, baked ginger, vinegar-processed rhizoma cyperi, fried bighead atractylodes rhizome and liquorice, wherein the baked ginger is bitter, pungent and warm, is good at warming the spleen and stomach yang, is not as pungent as the dried ginger in property and not as rapid as the dried ginger in property, but has mild and lasting effect, so that the traditional Chinese medicine is long used for warming the middle energizer to relieve pain, relieving diarrhea, warming channels to stop bleeding, and treating the main symptoms of deficiency-cold of the spleen and stomach and insufficiency of yang qi, and is a monarch medicine; the codonopsis pilosula is used for tonifying qi and spleen, the fried bighead atractylodes rhizome is used for tonifying spleen and eliminating dampness, the vinegar rhizoma cyperi is used for promoting qi circulation, relieving epigastric distention, soothing liver, relieving depression and relieving pain, and the combination of the three medicines can assist in increasing the middle-jiao warming capability of the baked ginger and can also relieve the symptoms of spleen damp stagnation, transport and transformation weakness, spleen and stomach swelling and stuffiness, inappetence and food consumption reduction, and are used as ministerial medicines; the salvia miltiorrhiza is red in color and bitter in taste, is good for entering blood system, can promote blood circulation to remove blood stasis, promote blood circulation to relieve pain, remove blood stasis to promote tissue regeneration, is used as an adjuvant drug in cooperation with monarch and ministerial drugs for treating pathological changes of swollen gland and stomach, bleeding and ulcer and promoting the recovery of spleen and stomach functions; licorice root, radix Glycyrrhizae, sweet in taste and warm in nature, is a guiding drug that can not only harmonize the effects of the other drugs, but also induce diuresis, and facilitate the discharge of water-dampness from urine. The medicines are compatible and have the effects of warming the middle-jiao and dispelling cold, and tonifying qi and spleen.

The invention has the beneficial effects that: the traditional Chinese medicine oral liquid is prepared by compounding 6 traditional Chinese medicines, has a simple formula, and is proved by experiments to have an obvious treatment effect on the chicken with the adenofibromyositis, and can improve the growth rate of the chicken, promote the secretion of pepsinogen and alpha-amylase, accelerate the elimination of the adenofibromyositis, promote the development of spleen and bursa of fabricius, and improve the immunity.

Drawings

FIG. 1 is a graph showing mental states and stool states of model group chickens of test example 1; as can be seen from the figure: model group chicken essence with poor state (figure A), loose and soil-discharging yellow manure (figure B, C);

FIG. 2 is a photograph of the glandular gastrectomy of the experimental example 1 model group and the blank group; as can be seen from the figure: swelling of glandular stomach (figure A), bleeding of glandular stomach mucous membrane, ulcer of muscular stomach cuticle (figure B), flat and vague papilla of glandular stomach mucous membrane (figure C, D); the stratum corneum of the muscular stomach has yellow color reduction and rough and uneven surface (figure D), and the blank group is normal (figure E);

FIG. 3 shows mental states and fecal status of the drug group and the blank group of chickens in test example 1; as can be seen from the figure: the chickens in the group with the medicine had better spirit and gradually normal feces (A, B picture); the chickens in the blank group had poor spirit, thin feces and reduced yellow pigment in toe paw (C, D diagram);

FIG. 4 shows the effect of the Chinese medicinal oral liquid on pepsinogen of a model chicken in test example 1; note: indicates significant difference (P < 0.05) compared to the blank group; # indicates significant difference compared to the model group (P < 0.05);

FIG. 5 shows the effect of the Chinese medicinal oral liquid on the serum alpha-amylase of a model chicken in test example 1; note: indicates significant difference (P < 0.05) compared to the blank group; # indicates significant difference compared to the model group (P < 0.05);

FIG. 6 shows clinical symptoms of experimental example 2, in which cockscomb, beard and toe yellow pigment are decreased and pale (A picture); dissecting glandular gastric mucosal hemorrhage, flat nipple, and vague tongue (B picture); ulcer of muscular stomach cuticle, yellow pigment decrease, lackluster (figure C), uneven pigment deposition on the surface of cuticle (figure D); the Chinese medicine group was basically normal after 7 days of drug withdrawal and the blank control group had still severe lesion (panel E).

Detailed Description

The effects of the examples and test examples are described below with reference to the drawings.

Example 1

The formula (parts by weight) is as follows: 15 portions of salvia miltiorrhiza, 20 portions of codonopsis pilosula, 20 portions of baked ginger, 10 portions of rhizoma cyperi with vinegar, 20 portions of fried rhizoma atractylodis macrocephalae and 15 portions of liquorice.

The production process comprises the following steps:

(1) weighing: weighing the raw material medicines according to the parts by weight;

(2) soaking and decocting: decocting the above 6 Chinese medicinal materials in water for 2 times. Adding 7 times of water for the first time, soaking for 2.5h, boiling for 1.0h, and filtering; adding 4.5 times of water for the second time, boiling for 0.5h, and filtering; the 2 filtrates were combined.

(3) Concentration: concentrating to obtain medicinal liquid with crude drug ratio of 1:1, standing for 12 hr, and filtering.

(4) Corrosion prevention and constant volume: the filtrate is preserved by adding 0.3 percent of sodium benzoate.

(5) Filling: and (6) filling and sealing.

The Chinese medicinal oral liquid of example 1 was used in the following test examples 1 to 3.

Test example 1: optimal formula screening of medicine for preventing and treating chicken glandular myositis

1 Material

1.1 drugs and reagents: the following three traditional Chinese medicine compositions are prepared. The traditional Chinese medicinal materials are purchased from Jinan Jianlian Chinese materia medica shop and prepared into oral liquid with the crude drug ratio of 1:1 according to the preparation requirement.

The formula I is as follows: 15 portions of salvia miltiorrhiza, 20 portions of codonopsis pilosula, 20 portions of baked ginger, 10 portions of vinegar-processed rhizoma cyperi (vinegar-processed), 20 portions of fried rhizoma atractylodis macrocephalae (fried), and 15 portions of liquorice;

and the formula II comprises: 15 portions of salvia miltiorrhiza, 20 portions of codonopsis pilosula, 10 portions of baked ginger, 10 portions of cimicifuga foetida, 20 portions of fried bighead atractylodes rhizome (fried), 3 portions of monkshood, 5 portions of common andrographis herb and 15 portions of liquorice;

the formula III is as follows: radix codonopsitis 28, fried white 19, tuckahoe 19, cimicifuga rhizome 9, costus root 9, salvia miltiorrhiza 9 and dried orange peel 7.

Rhubarb, purchased from the Jianlian Chinese medicine store in Jinan province, is prepared into water decoction with the crude drug ratio of 1:1 for later use; polyethylene glycol-400, batch number 20010306, manufactured by Tanjin quartz clock factory, Bazhou chemical division; the pepsinogen and alpha-amylase detection kit is purchased from Jiangsu Jingmei biotechnology limited; ultra-low temperature freezer at-80 ℃ from Mike Mitsubishi, Inc.; an electric heating constant temperature water bath which is purchased from Shanghai sperm macro test equipment Co., Ltd; a low speed centrifuge from Shanghai' an pavilion scientific instruments factory; optical microscopes, product of Chongqing Chongguang industries, Inc.; a high-speed cryogenic refrigerated centrifuge available from Thermo Fisher Scientific, usa; full-automatic enzyme labeling analyzer, Australian Osheng Instrument Co.

1.2SPF cocks 20 days old, available from Shandong Hao Tai laboratory animal center; the compound feed AC820R is produced by feed Limited in Jinan pond of Jinan, and the crude protein is more than or equal to 15%; SPF chicken isolators, manufactured by von experimental animal facilities limited, suzhou.

2 method

2.1 grouping: selecting 20-day-old SPF cocks with the weight fluctuating up and down by 5%, randomly dividing the SPF cocks into three groups from one square to three squares, a model group and a blank group, weighing and numbering respectively, and weighing 45 chickens in each group.

2.2 model construction: except for the blank group, 1.0 percent of polyethylene glycol-400 and 2.5mL/kg.w of rhubarb water decoction are respectively added into drinking water of each group, and are continuously added for 10 days, drinking in the morning and changing tap water in the afternoon to construct the glandular muscle gastritis model.

2.3 administration: after the model is successfully constructed, 2mL/kg of body weight of the Chinese medicinal oral liquid of the first formula, the second formula and the third formula is added into drinking water respectively, the drinking water is continuously drunk for 7 days, and no medicament is added into the model group and the blank group.

2.4 serum assay: separating serum by blood procoagulant, and respectively measuring the contents of pepsinogen and alpha-amylase.

2.5 data analysis: in the test, the fecal character and mental state of each group of chickens are observed in detail, and the weight of each group is recorded. And (3) constructing a model, finishing the administration, stopping the administration for 7 days, randomly performing per group autopsy on 15 patients, respectively counting indexes such as glandular myogastric lesion rate, weight gain rate, pepsinogen and alpha-amylase content and the like, and analyzing the difference by using SPPS.

Judging standard of proventriculitis lesion: when the spiritual loss occurs, the feces are thin; swelling of the glandular stomach, thickening of the glandular stomach wall, swelling of the mucosal papilla, flattening, blurring or ulcerated bleeding, all are considered to be onset of disease.

Judging standard of the pathological changes of the muscular gastritis: the incidence of yellow pigment in the skin of the muscle stomach is judged to be hypopigmentation, lusterless, rough and uneven surface, chapping and ulcer, even ulcer under the skin of the muscle stomach.

3 results

3.1 model construction

3.1.1 clinical symptoms: the model group has poor chicken essence state (figure 1A), and has yellow manure (figure 1B, C) after being diluted and discharged; the cesarean examination shows the enlargement of the glandular stomach (fig. 2A), the bleeding of the glandular stomach mucous membrane (fig. 2B), and the flat and vague papilla of the glandular stomach mucous membrane (fig. 2C, D); sarcogastric stratum corneum yellow declined, rough and uneven surface (fig. 2D), ulceration (fig. 2B), blank group normal (fig. 2E). The model accords with the clinical and caesarean section characteristics of the chicken gland muscular gastritis.

3.1.2 when the model is successfully constructed, the weight gain rate, the variability of the glandular disease, the variability of the muscular disease, the serum pepsin and the alpha-amylase of the model chicken

TABLE 1 weight gain, variability of adenoid gastropathy and serum pepsinogen and alpha-amylase (n ═ 45) in model and placebo chickens

Note: indicates significant difference from the same column (p < 0.05); differences were very significant (p < 0.01).

As can be seen from Table 1, the weight gain rate of the model chicken is 33.06 percentage points lower than that of the blank group, and the difference is very obvious (p is less than 0.01); the incidence rate of glandular muscle stomach is more than 80%, and the difference is very obvious (p is less than 0.01); the pepsinogen is remarkably lower than 7.52 percentage points of a blank group, and the difference is remarkable (p is less than 0.05); the alpha-amylase content was 35.3 percentage points lower than the blank, but the difference was not significant (p > 0.05).

3.2 Effect of Chinese herbs on Chicken

3.2.1 clinical symptoms: the chickens in the group with the medicine had better spirits and gradually normal feces (fig. 3A, B); the model group had poor spirit, thin stool and yellow pigment declined in the toe (fig. 3C, D).

3.2.2 Effect of Chinese herbs on the weight gain of glandular-muscular gastritis model chickens

TABLE 2 influence of Chinese medicine on the weight gain of glandular-muscular gastritis model chicken (%)

As can be seen from Table 2, the weight gain rates of the first, second and third groups of prescription in the medication period are respectively 9.2 percent, 8.8 percent and 9.9 percent higher than those of the model group; the first, second and third groups of the prescription at the drug withdrawal period are respectively 6.0 percent, 0.2 percent and 3.3 percent higher than the model group; the first, second and third groups of the prescription in the medication period and the drug withdrawal period are respectively 13.6 percent, 4.9 percent and 10.6 percent higher than those in the model group.

3.2.3 Effect of Chinese herbs on Chicken serum pepsinogen

As can be seen from fig. 4, the pepsinogen content in the model group and the two square groups in the administration period (data at the end of administration, the same below) is significantly lower than that in the blank group (P < 0.05), the pepsinogen content in the two square groups and the three square groups is not significantly different from that in the blank group (P > 0.05), and the pepsinogen content in the one square group and the three square groups is slightly higher than that in the three square groups; in the recovery period (data of 7 days after drug withdrawal, the same below), the pepsinogen content of the model group is obviously lower than that of the blank group (P is less than 0.05), the pepsinogen content of the formula group is obviously higher than that of the model group (P is less than 0.05), and the pepsinogen content of the formula group, the formula group and the formula group is not obviously different from that of the blank group (P is more than 0.05).

3.2.4 Effect of Chinese herbs on Chicken serum alpha-amylase

As can be seen from FIG. 5, during the administration period, the content of alpha-amylase in the model group is significantly lower than that in the blank group (P < 0.05), and the content of alpha-amylase in the square group, the square group and the square group is significantly higher than that in the model group (P < 0.05); during the recovery period, the content of alpha-amylase in the square group is the highest, but the difference between the groups is not significant (P is more than 0.05).

4 conclusion and analysis

4.1 Drinking water with rhubarb and polyethylene glycol can successfully construct a chicken glandular myositis model with slow growth, glandular gastric mucosa edema, hemorrhage, ulcer, flat and vague glandular gastric papilla, and hypo-yellow pigment, dull and rough surface, even ulcer, and obviously reduced content of serum pepsinogen and alpha-amylase.

4.2 the traditional Chinese medicine oral liquid prepared by the first traditional Chinese medicine prescription has the effects of improving the growth rate of model chickens, promoting the secretion of pepsinogen and alpha-amylase and accelerating the elimination of glandular myogastric inflammation, and compared with the second prescription and the third prescription, the prescription is simpler (only compounded by 6 traditional Chinese medicines), so the traditional Chinese medicine oral liquid is the best prescription for preventing and treating the chicken glandular myogastritis.

Test example 2: the traditional Chinese medicine oral liquid of the invention has the influence on the adenomuscular gastritis of the Kebao broiler chicken

1 Material

1.1, traditional Chinese medicine: the traditional Chinese medicine oral liquid prepared by the first formula has a crude drug ratio of 1: 1; chickens, 15-day-old broiler chickens of Kebao family goods bred in three-layer cages by Shandong oasis farming-grazing Co., Ltd; the feed, broiler early stage (broiler chicken) mixed feed 510, produced by feed science and technology ltd in linyi oasis.

2 method

2.1 clinical diagnosis: the yellow pigment of the rooster comb, the meat beard and the toe of the whole group of broilers is reduced and pale (figure 6A); dissecting glandular gastric mucosal hemorrhage, flat nipple, vague (fig. 6B); ulceration of the stratum corneum of the muscular stomach, yellowish pigmentation, dull luster (fig. 6C), uneven and rough pigmentation of the stratum corneum surface (fig. 6D). Diagnosing as adenomyositis.

2.2 grouping: selecting chickens in the adjacent cages of the middle layer, weighing one by one, randomly dividing the chickens within the range of 95-105% of the standard weight into a traditional Chinese medicine group and a blank group, wherein each group comprises 90 chickens, each cage comprises 30 chickens, and each chicken is provided with a foot number one by one.

2.3 administration: the medicine group drinks water according to 1mL/kg body weight for 5 days continuously, and the blank group drinks tap water; and (4) observation: continuously observing for 7 days after the administration, weighing and counting the weight gain rate one by one in the administration period and the observation period, randomly selecting 30 chickens for killing before the administration, after the administration and after the observation, separating serum to measure the content of pepsin, alpha-amylase and SigA (secretory immunoglobulin A), observing the pathological change of viscera, separating and weighing the weight of the glandular stomach, the muscular stomach, the spleen and the bursa of Fabricius, and calculating the corresponding organ index.

3 results

3.1 clinical symptoms: after the medicine is taken for 5 days, the spirit of the large crowd is improved, and the food intake is increased. The lesion rate of glandular gastrulation of the Chinese medicinal group in the caesarean section is obviously lower than that of the blank group, the Chinese medicinal group in the caesarean section is basically normal after 7 days of drug withdrawal (figure 6E), the lesion of the blank group is still serious (figure 6F), but the death does not occur.

3.2 Effect of Chinese herbs on weight gain of broiler chickens

TABLE 3-1 Effect of Chinese herbs on weight gain and weight gain ratio of chickens (n ═ 30)

As can be seen from Table 3-1, the weight gain and the weight gain rate of the Chinese medicine groups in the administration period, the withdrawal period and the whole period are respectively 12.13g, 33.84g, 44.15g, 4.21, 5.08 and 15.42 percent higher than those of the blank group.

3.3 Effect of Chinese herbs on Chicken organ index

TABLE 3-2 Effect of Chinese herbs on Chicken adenogastric index, spleen index and bursa of Fabricius index (n ═ 30)

As can be seen from Table 3-2, the adenogastric index, spleen index and bursal index of the traditional Chinese medicine in the administration period are respectively 1.07%, 0.03% and 0.45 higher than those of the blank group, and are respectively 105.10%, 103.33% and 126.63% of the blank group; the glandular gastric index, the spleen index and the bursal index of the traditional Chinese medicine in the recovery period are respectively 2.18 percent, 0.2 percent and 0.43 percent higher than those of a blank group, and are respectively 111.94 percent, 119.05 percent and 129.25 percent of the blank group.

3.4 Effect of Chinese herbs on the content of pepsin, alpha-amylase and SIgA in the serum of broilers

As can be seen from tables 3-3, no significant difference was observed between the pepsin content and alpha-amylase content in the two groups during the administration period and the blank group (p > 0.05); the pepsin content and the alpha-amylase content of the Chinese medicine in the recovery period are obviously higher than those of a blank group (P is less than 0.05); the content of the traditional Chinese medicine SIgA in the medication period and the recovery period is remarkably higher than that in a blank group (p is less than 0.01).

TABLE 3-3 Change in pepsin and alpha-amylase content (pg/mL) in serum during dosing and recovery periods

Note: p < 0.05, p < 0.01 compared to the blank group.

And 4, conclusion: the traditional Chinese medicine has obvious treatment effect on the chicken suffering from the adenofibromyositis, and has the effects of promoting growth, promoting spleen and bursal development, improving immunity and accelerating elimination of the adenofibromyositis.

Test example 3: curative effect of traditional Chinese medicine on glandular-muscular gastritis hybrid broiler chicken

Materials (I) and (II)

1. Chicken flocks: 10000 broilers aged 17 days in Jiyang county of Jinan City are listless, the feed intake is reduced from normal 800kg to 680kg, the weight of large groups is greatly different, weak chickens are more, the yellow color of cockscomb, legs and beaks is faded away, and the yellow water sample mixed with feed scraps is rare. The local diagnosis of proventriculitis is that the proventriculitis is treated by the medicine factory, and the medicine is ineffective.

2. Medicine preparation: the traditional Chinese medicine oral liquid is 500 mL/bottle; a liquid medicine for replenishing gold (compound liquid vitamin), a product of Shandong Hao Tai science and technology pharmaceutical industry Limited company, 100 mL/bottle.

II, diagnosis

1. And (4) checking: the mental state is still, the size difference is large, and the yellow color of the legs and the beaks is faded to be offwhite.

2. C, performing autopsy: glandular stomach swelling like globoid, section eversion, mucosa edema, nipple blurring; the yellow color of the stratum corneum of the muscular stomach declines, a circular ulcer focus exists, and other organs have no obvious pathological changes.

3, preliminary diagnosis: adenomuscular gastritis.

Thirdly, prevention measures: the oral liquid of the invention is 500 oral liquid/bottle for 5 days, and each bottle of the liquid for replenishing gold (liquid vitamin) is mixed with 500 jin of water for continuous use.

And fourthly, obtaining a result: on the 2 nd day of administration, the food consumption is increased from 680kg to 750kg before administration, the feces are thickened and changed from yellow to grey, the feces are gradually normal on the 3 rd day of administration, and on the 5 th day of administration, the food consumption is restored to 850kg, the mental state is normal, and the feces are normal.

Claims (8)

1. The traditional Chinese medicine oral liquid for preventing and treating chicken glandular myositis is characterized by being prepared from the following raw material medicines in parts by weight: 5-15 parts of salvia miltiorrhiza, 10-20 parts of codonopsis pilosula, 15-20 parts of baked ginger, 3-10 parts of vinegar rhizoma cyperi, 5-20 parts of fried bighead atractylodes rhizome and 10-15 parts of liquorice.

2. The traditional Chinese medicine oral liquid for preventing and treating chicken adenofibromyositis of claim 1, which is characterized by comprising the following raw material medicines in parts by weight: 15 portions of salvia miltiorrhiza, 20 portions of codonopsis pilosula, 20 portions of baked ginger, 10 portions of rhizoma cyperi with vinegar, 20 portions of fried rhizoma atractylodis macrocephalae and 15 portions of liquorice.

3. The preparation method of the traditional Chinese medicine oral liquid according to claim 1 or 2, which is characterized in that the raw materials are weighed according to the parts by weight, decocted with water for 2-3 times, and concentrated to a liquid medicine with the raw material mass ratio of 1: 0.5-1.5.

4. The preparation method of the traditional Chinese medicine oral liquid as claimed in claim 3, which is characterized in that the decoction is carried out for 2 times, and specifically comprises the following steps: adding 5-10 times of mass of water for the first time, soaking for 2-3 h, boiling for 0.5-1.5 h, and filtering; adding 3-5 times of water by mass for the second time, boiling for 0.1-1.0 h, and filtering; the 2 filtrates were combined.

5. The method for preparing a Chinese medicinal oral liquid according to claim 4, wherein the liquid medicine is preserved by adding a preservative.

6. The preparation method of the traditional Chinese medicine oral liquid as claimed in claim 5, which is characterized by comprising the following steps:

(1) weighing: weighing the raw material medicines according to the parts by weight;

(2) soaking and decocting: the 6 medicines are decocted twice by adding water, 5 to 10 times of water by mass is added for the first time, the mixture is soaked for 2 to 3 hours, boiled for 0.5 to 1.5 hours and filtered; adding 3-5 times of water by mass for the second time, boiling for 0.1-1.0 h, and filtering; mixing the filtrates for 2 times;

(3) concentration: concentrating the mixture to obtain a liquid medicine with the mass ratio of the crude drug of 1: 0.8-1.2, standing and filtering;

(4) corrosion prevention and constant volume: adding preservative into the filtrate for corrosion prevention;

(5) filling: and (6) filling and sealing.

7. The method for preparing a Chinese medicinal oral liquid according to claim 6, wherein the liquid is concentrated to a liquid medicine with a crude drug mass of 1: 1.

8. The use of the Chinese medicinal oral liquid of claim 1 or 2 in preventing and treating chicken adenofibromyositis.

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