CN114306516B - Traditional Chinese medicine decoction pieces rich in gastrodin and production process thereof - Google Patents
Traditional Chinese medicine decoction pieces rich in gastrodin and production process thereof Download PDFInfo
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Abstract
The invention relates to a Chinese medicinal decoction piece rich in gastrodin and its preparation method, in the field of Chinese medicinal processing technique, steam rhizoma Gastrodiae first, oven dry slice rhizoma Gastrodiae Preparata, soak with rhizoma Gastrodiae extract and oven dry finally, make decoction piece; according to the method, the characteristics of the Gastrodia elata of each grade are utilized, first-class Gastrodia elata with high content of Gastrodia elata polysaccharide is used as a decoction piece substrate, gastrodia elata extract is prepared from four-class Gastrodia elata with similar gastrodin content to the first-class Gastrodia elata, and the Gastrodia elata extract is absorbed into Gastrodia elata slices, so that the Gastrodia elata slices are rich in gastrodin; the process provided by the invention also adopts various methods to solve the problems of blackening and warping of the gastrodia elata; in the preparation of the rhizoma gastrodiae extract, raw rhizoma gastrodiae is pressed into pulp to prevent gelatinization in the extraction process caused by modification of starch in steamed rhizoma gastrodiae powder from influencing the extraction effect, and during extraction, amylase is used for enzymolysis of starch in the rhizoma gastrodiae pulp, and then a wall-breaking compound enzyme is used for dissolving cell walls to fully release gastrodin, so that the content of the gastrodin in the rhizoma gastrodiae extract is high.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine processing, and particularly relates to a gastrodin-rich traditional Chinese medicine decoction piece and a production process thereof.
Background
Rhizoma gastrodiae is a rare traditional Chinese medicine, and the traditional Chinese medicine considers that the rhizoma gastrodiae has the main effects of calming wind and relieving spasm, calming liver yang, dispelling wind and dredging collaterals. Modern pharmacological studies show that gastrodin has the effects of increasing sedation, hypnosis, analgesia, immunity enhancement and the like, and the gastrodin is a water-soluble substance, and the gastrodia elata is often processed into decoction pieces in the prior art so as to be convenient to take.
The gastrodia elata is divided into first-class gastrodia elata, second-class gastrodia elata and fourth-class gastrodia elata in sequence according to the mass size, wherein the first-class gastrodia elata is large in volume and mass and serves as a first-class product in the gastrodia elata, gastrodin and gastrodin are rich in the gastrodia elata, the price is high, the content of the gastrodin in the fourth-class gastrodia elata serves as a lower-class gastrodia elata, the content of the gastrodin in the fourth-class gastrodia elata is close to that of the first-class gastrodia elata, and the content of gastrodin in the fourth-class gastrodia elata is low.
Disclosure of Invention
In order to solve the technical problems mentioned in the background technology, the invention aims to provide a gastrodin-rich traditional Chinese medicine decoction piece and a production process thereof.
The purpose of the invention can be realized by the following technical scheme:
a production process of a traditional Chinese medicine decoction piece rich in gastrodin comprises the following working procedures:
step S1: cleaning first-class rhizoma Gastrodiae, soaking in clear water, taking out rhizoma Gastrodiae, draining, pricking gas needle holes on the upper surface of rhizoma Gastrodiae with needle roller, steaming in microwave steam box, and steaming rhizoma Gastrodiae to obtain cooked rhizoma Gastrodiae;
the moisture of each part inside the rhizoma gastrodiae is uniform after the rhizoma gastrodiae is soaked in water, and the rhizoma gastrodiae is steamed in cooperation with microwave, so that each part inside the rhizoma gastrodiae is uniformly heated, the steaming time is short, compared with the traditional water-resisting steaming, the phenomenon that a finished product is blackened due to long-time excessive heating of a surface layer is avoided, meanwhile, the steam generated by the microwave steaming is discharged from an air through hole, the problem that the surface of the rhizoma gastrodiae is cracked due to the traditional steaming is avoided, in addition, the microwave steaming is used for heating in a ventilation environment, the steam is discharged in time, and the rhizoma gastrodiae is pre-dried;
step S2: hanging one end of the cooked gastrodia elata, vertically suspending the end of the cooked gastrodia elata, putting the end into a drying box for drying treatment, drying until the water content is 28-35%, taking down the gastrodia elata, and cutting the gastrodia elata into gastrodia elata slices; the cooked gastrodia elata is dried in the air, so that moisture dried from the gastrodia elata is evaporated and removed in time, the problem that in the traditional drying process, moisture at the contact part of the gastrodia elata and a drying net cannot be dried in time, the part is in a wet state for a long time, the part is blackened at the drying temperature, the color and luster of a finished product are uneven, the product appearance is influenced is avoided, the gastrodia elata slices with the water content of 28-35% have good toughness by combining production experience, and the peripheries of the sliced gastrodia elata slices are not easy to warp in the state;
step S3: naturally drying the gastrodia elata slices until the water content is not more than 15%, then placing the gastrodia elata slices into a soaking tank, adding the gastrodia elata extract into the soaking tank for soaking for 5-10 hours at room temperature to enable the gastrodia elata slices to fully absorb the gastrodia elata extract, taking out the gastrodia elata slices, draining, carrying out secondary drying, and carrying out secondary drying until the water content is not more than 7% to obtain the gastrodin-rich traditional Chinese medicine decoction pieces.
Further, in the step S2, the drying temperature of the drying treatment is 80-90 ℃, and hot air drying is adopted, and the air speed is properly adjusted according to the arrangement density of the cooked gastrodia elata.
Further, in the step S3, the temperature of secondary baking is 40-50 ℃, and the low-temperature slow drying effectively prevents the edge of the gastrodia elata piece from being warped due to too fast dehydration at the edge.
Further, the gastrodia elata extracting solution is prepared by the following steps:
step A1: squeezing and filtering rhizoma Gastrodiae, washing the filter cake with distilled water, filtering, and mixing filtrates to obtain rhizoma Gastrodiae slurry;
step A2: adding amylase into the gastrodia elata pulp, performing enzymolysis on starch in the gastrodia elata pulp to avoid that a large amount of starch in the gastrodia elata pulp is gelatinized to influence extraction of gastrodin, then adding a wall-breaking compound enzyme into the gastrodia elata pulp to dissolve cell walls of the gastrodia elata in the gastrodia elata pulp, facilitating full release of the gastrodin, standing and precipitating after enzyme deactivation at high temperature, taking an upper turbid liquid layer, filtering, and taking filtrate to obtain mother liquor;
step A3: adding ethanol solution into the mother liquor, performing low-frequency ultrasonic extraction at 28kHz for 3h, adding sodium bicarbonate to adjust the pH value of the mixed solution to 7.5-8.5, standing for 24h, centrifuging the mixed solution, collecting supernatant, and recovering part of ethanol from the supernatant under reduced pressure to obtain rhizoma Gastrodiae extract.
Further, in the working procedure A2, the amylase is alpha-amylase, the dosage of the amylase is 2.7-3.3mg/L, the enzymolysis temperature is 38-42 ℃, and the enzymolysis time is 2 hours.
Further, in the step A2, the wall-breaking compound enzyme comprises pectinase, xylanase and protease, and the mass ratio of the pectinase to the xylanase to the protease is 1:0.45-0.5, 0.1-0.15, the dosage of the wall-breaking compound enzyme is 4.7-6.1mg/L, the enzymolysis temperature is 35-40 ℃, and the enzymolysis time is 3h.
Further, in the step A3, the volume concentration of the ethanol solution is 70%, and the volume ratio of the ethanol solution to the mother liquor is 1.1 to 1.25.
The invention has the beneficial effects that:
1. the method reasonably utilizes the characteristics of the Gastrodia elata of each grade, uses first-grade Gastrodia elata with high Gastrodia elata polysaccharide content as a decoction piece matrix, uses four-grade Gastrodia elata with gastrodin content similar to that of the first-grade Gastrodia elata to prepare Gastrodia elata extract, dries Gastrodia elata slices, then soaks the Gastrodia elata extract to enable the Gastrodia elata extract to be absorbed into Gastrodia elata slices, and finally dries the Gastrodia elata slices to enable the Gastrodia elata slices to be rich in Gastrodin, and meanwhile, the Gastrodia elata slices are made of first-grade Gastrodia elata, wherein the Gastrodia elata polysaccharides are rich.
2. The process provided by the invention adopts various methods to prevent the problems of blackening and warping of the gastrodia elata, such as: soaking and microwave steaming are adopted, the steaming time is short, the steaming is uniform and is in an environment with low water content, and the problem that the surface layer of the gastrodia elata is excessively heated for a long time to cause blackening of a finished product is effectively solved; the cooked gastrodia elata is dried in a suspended mode, so that the problem that moisture in the contact position of the gastrodia elata and a drying net cannot be timely dried to cause blackening in the traditional drying process is solved.
3. The invention provides a preparation method of a gastrodia elata extract rich in gastrodin, which is characterized in that raw gastrodia elata is pressed into pulp, the starch modification in steamed gastrodia elata powder is prevented from causing gelatinization in the extraction process to influence the extraction effect, the amylase is used for carrying out enzymolysis on the starch in the gastrodia elata pulp during extraction, then a wall-breaking compound enzyme is used for dissolving cell walls, the gastrodin is fully released, and the gastrodin content in the gastrodia elata extract is high.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
In this embodiment, a specific implementation process of preparing a gastrodia elata extracting solution is as follows:
step A1: adding 100kg of Gastrodia elata Blume into a juicer, assembling a 16-mesh screen, squeezing and filtering Gastrodia elata Blume, repeatedly washing and filtering filter cakes for 3 times by adopting 100L of distilled water, and combining filtrates to obtain Gastrodia elata Blume pulp;
step A2: adding 0.324g of commercially available alpha-amylase into the gastrodia elata pulp, fully stirring and mixing, slowly heating to 38 ℃, preserving heat and performing enzymolysis for 2 hours, performing enzymolysis on starch in the gastrodia elata pulp, avoiding the influence of gelatinization of a large amount of starch in the gastrodia elata pulp on extraction of gastrodin, and taking pectinase, xylanase and protease according to the mass ratio of 1:0.45, preparing a wall-broken compound enzyme by mixing, adding 0.564g of the wall-broken compound enzyme into rhizoma gastrodiae slurry, fully stirring and mixing, regulating and controlling the temperature to 35 ℃, performing heat preservation and enzymolysis for 3 hours, performing steam enzyme deactivation after the enzymolysis is finished, standing until obvious layering appears, filtering an upper turbid liquid layer by using a 80-mesh screen, and taking a filtrate to obtain a mother solution;
step A3: measuring mother liquor, adding an ethanol solution with the volume concentration of 70%, controlling the volume ratio of the ethanol solution to the mother liquor to be 1.1, carrying out low-frequency ultrasonic extraction for 3h at 28kHz, adding sodium bicarbonate to adjust the pH value of the mixed liquor to be 7.5, standing for 24h, centrifuging, taking supernatant, recovering ethanol under reduced pressure, and concentrating to 60% of the volume of the original supernatant to obtain the rhizoma gastrodiae extract.
Example 2
In this embodiment, a specific implementation process of preparing a gastrodia elata extracting solution is as follows:
step A1: adding 100kg of four equal rhizoma Gastrodiae into a juicer, assembling 16 mesh sieve, squeezing and filtering rhizoma Gastrodiae, washing and filtering filter cake with 100L of distilled water for 3 times, and mixing filtrates to obtain rhizoma Gastrodiae slurry;
step A2: adding 0.396g of commercially available alpha-amylase into the gastrodia elata pulp, fully stirring and mixing, slowly heating to 42 ℃, preserving heat and performing enzymolysis for 2 hours, performing enzymolysis on starch in the gastrodia elata pulp, avoiding that a large amount of starch in the gastrodia elata pulp is gelatinized to influence extraction of gastrodin, and taking pectinase, xylanase and protease according to the dosage-mass ratio of 1:0.5, mixing to prepare a wall-broken compound enzyme, adding 0.732g of the wall-broken compound enzyme into the gastrodia elata pulp, fully stirring and mixing, regulating and controlling the temperature to be 40 ℃, performing heat preservation and enzymolysis for 3 hours, performing steam enzyme deactivation after the enzymolysis is finished, standing until obvious layering occurs, filtering an upper turbid liquid layer by using a 80-mesh screen, and taking filtrate to obtain mother liquor;
step A3: measuring mother liquor, adding an ethanol solution with the volume concentration of 70%, controlling the volume ratio of the ethanol solution to the mother liquor to be 1.25, ultrasonically extracting at the low frequency of 28kHz for 3h, then adding sodium bicarbonate to adjust the pH value of the mixed liquor to be 8.5, standing for 24h, centrifuging, taking supernatant, recovering ethanol under reduced pressure, and concentrating to 60% of the volume of the original supernatant to obtain the rhizoma gastrodiae extract.
Example 3
The embodiment of the preparation of the gastrodin-rich traditional Chinese medicine decoction piece comprises the following specific implementation processes:
step S1: cleaning 50kg of first-class rhizoma gastrodiae, placing the cleaned first-class rhizoma gastrodiae in clear water, soaking the cleaned first-class rhizoma gastrodiae for 10h, taking out the rhizoma gastrodiae, draining, tying a gas passing pinhole on the upper surface of the rhizoma gastrodiae by using a roller pin, then placing the rhizoma gastrodiae into a microwave steam box for steaming, and steaming the rhizoma gastrodiae to obtain cooked rhizoma gastrodiae;
the gastrodia elata is soaked in water, water in each part of the inside of the gastrodia elata is uniform, the gastrodia elata is steamed in a matched mode, each part in the gastrodia elata is heated uniformly, steaming time is short, compared with traditional water-resisting steaming, the phenomenon that a finished product is blackened due to the fact that a surface layer is heated excessively for a long time is avoided, meanwhile, water vapor generated by microwave steaming is discharged from an air through pinhole, the problem that the surface of the gastrodia elata is cracked due to the traditional steaming is avoided, in addition, the microwave steaming is used for heating in a ventilating environment, the water vapor is discharged in time, and the gastrodia elata is pre-dried;
step S2: hanging one end of the cooked gastrodia elata, vertically suspending the end of the cooked gastrodia elata, enabling the distance between every two adjacent hooks to be 5cm, placing all the cooked gastrodia elata into a drying box after hanging, setting the drying temperature to be 80 ℃ and the air speed to be 12m/min, drying the cooked gastrodia elata until the water content is 28%, taking down the gastrodia elata, placing the gastrodia elata into a slicing machine, setting the slicing thickness to be 3mm, and cutting the gastrodia elata into gastrodia elata slices;
the cooked gastrodia elata is dried in the air, so that moisture dried from the gastrodia elata is timely evaporated and removed, the problem that in the traditional drying process, moisture of a contact part of the gastrodia elata and a drying net cannot be timely dried and removed, the part is in a wet state for a long time, the part is blackened at the drying temperature, the color and luster of a finished product are uneven, the product appearance is influenced, the gastrodia elata piece with the water content of 28% also has good toughness by combining with production experience, and the periphery of the sliced gastrodia elata piece is not easy to warp in the state is solved;
step S3: naturally drying the gastrodia elata slices until the water content is not more than 15%, then placing the gastrodia elata slices into a soaking tank, adding the gastrodia elata extracting solution prepared in the embodiment 1 into the soaking tank for soaking at room temperature for 5 hours to enable the gastrodia elata slices to fully absorb the gastrodia elata extracting solution, taking out the gastrodia elata slices, draining, carrying out secondary drying, setting the drying temperature to be 40 ℃, and carrying out secondary drying until the water content is not more than 7% to obtain the gastrodin-rich traditional Chinese medicine decoction pieces.
Example 4
The embodiment of preparing the traditional Chinese medicine decoction pieces rich in gastrodin specifically comprises the following implementation processes:
step S1: cleaning 50kg of first-class rhizoma Gastrodiae, soaking in clear water for 10h, taking out the rhizoma Gastrodiae, draining, tying an air-passing pinhole on the upper surface of the rhizoma Gastrodiae with needle roller, steaming in a microwave steam box, and steaming the rhizoma Gastrodiae to obtain cooked rhizoma Gastrodiae;
step S2: hanging one end of the cooked gastrodia elata, suspending the end of the cooked gastrodia elata vertically, enabling the distance between adjacent hooks to be 7cm, placing all the cooked gastrodia elata into a drying box after hanging, setting the drying temperature to be 90 ℃ and the air speed to be 16m/min, drying the cooked gastrodia elata until the water content is 35%, taking down the gastrodia elata, placing the gastrodia elata into a slicing machine, setting the slicing thickness to be 3mm, and cutting into gastrodia elata slices;
step S3: naturally drying the gastrodia elata pieces until the water content is not more than 15%, then placing the gastrodia elata pieces into a soaking tank, adding the gastrodia elata extracting solution prepared in the embodiment 2 into the soaking tank for soaking at room temperature for 10 hours to enable the gastrodia elata pieces to fully absorb the gastrodia elata extracting solution, taking out the gastrodia elata pieces, draining, carrying out secondary drying, setting the drying temperature to be 50 ℃, and carrying out secondary drying until the water content is not more than 7% to obtain the gastrodin-rich traditional Chinese medicine decoction pieces.
Comparative example 1
The comparative example is a processing technology of rhizoma gastrodiae decoction pieces in the prior art, and the specific implementation process is as follows:
step 1: removing impurities from first-class rhizoma Gastrodiae, and cleaning to obtain cleaned rhizoma Gastrodiae;
and a step 2: naturally moistening the cleaned rhizoma Gastrodiae for 4 hr to soften and cut rhizoma Gastrodiae to obtain softened rhizoma Gastrodiae;
step 3: slicing the softened rhizoma Gastrodiae into slices with thickness of 3mm by a slicing machine to obtain rhizoma Gastrodiae wet slices;
and step 4: and flatly paving the wet gastrodia elata slices on a drying net, drying by adopting circulating hot air at the drying temperature of 60 ℃ until the water content is not more than 10%, and thus obtaining the gastrodia elata decoction pieces.
The pieces prepared in example 3, example 4 and comparative example 1 were evaluated for appearance color and warpage, and the specific data are shown in Table 1:
TABLE 1
As can be seen from table 1, the decoction pieces of gastrodia elata prepared in examples 3 and 4 showed uniform pale yellow color with slight warpage in the edge position.
100g of each of the rhizoma gastrodiae decoction pieces prepared in example 3, example 4 and comparative example 1 was taken, soaked in 1L of water for 1 hour, then boiled at normal pressure for 20 minutes, cooled, and subjected to gastrodin content measurement on the boiled extract by referring to high performance liquid chromatography specified in the "chinese pharmacopoeia", as shown in the specific data table 2:
TABLE 2
As can be seen from Table 2, the content of gastrodin in the boiling leachate of rhizoma Gastrodiae decoction pieces prepared in example 4 was 311mg/L, which is much higher than that of the product produced by the prior art.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing is illustrative and explanatory only and is not intended to be exhaustive or to limit the invention to the precise embodiments described, and various modifications, additions, and substitutions may be made by those skilled in the art without departing from the scope of the invention or exceeding the scope of the claims.
Claims (7)
1. The production process of the gastrodia elata decoction pieces rich in gastrodin is characterized by comprising the following steps of:
step S1: cleaning first-class rhizoma Gastrodiae, soaking, draining, pricking gas-passing pinholes on the upper surface of the rhizoma Gastrodiae, and microwave steaming to obtain cooked rhizoma Gastrodiae;
step S2: hanging one end of the cooked gastrodia elata, drying in the air until the water content is 28-35%, and cutting to obtain gastrodia elata slices;
step S3: drying the gastrodia elata slices until the water content is not more than 15%, then adding the gastrodia elata extract for soaking at room temperature for 5-10h, finally taking out the gastrodia elata slices, draining, and drying for the second time until the water content is not more than 7% to obtain gastrodia elata decoction pieces rich in gastrodin;
the rhizoma gastrodiae extract is prepared by the following steps:
step A1: squeezing and filtering rhizoma Gastrodiae, washing filter cake, filtering, and mixing filtrates to obtain rhizoma Gastrodiae slurry;
step A2: adding amylase into rhizoma Gastrodiae slurry, performing enzymolysis on starch in rhizoma Gastrodiae slurry, adding wall-broken complex enzyme into rhizoma Gastrodiae slurry, dissolving cell wall of rhizoma Gastrodiae in rhizoma Gastrodiae slurry, inactivating enzyme at high temperature, standing for precipitation, filtering the upper turbid liquid layer, and collecting filtrate to obtain mother liquor; the wall-broken compound enzyme comprises pectinase, xylanase and protease;
step A3: adding ethanol solution into the mother liquor, performing ultrasonic extraction for 3h, adjusting the pH value of the mixed solution to 7.5-8.5, standing for 24h, centrifuging the mixed solution, taking supernatant, and concentrating the supernatant under reduced pressure to obtain rhizoma Gastrodiae extract.
2. The production process of the gastrodin-rich gastrodia elata decoction pieces as claimed in claim 1, wherein in the step A2, the amylase is alpha-amylase, the dosage of the amylase is 2.7-3.3mg/L, the enzymolysis temperature is 38-42 ℃, and the enzymolysis time is 2 hours.
3. The production process of the gastrodin-rich gastrodia elata decoction pieces as claimed in claim 1, wherein in the step A2, the dosage mass ratio of pectinase, xylanase and protease is 1:0.45-0.5, the dosage of the wall-breaking compound enzyme is 4.7-6.1mg/L, the enzymolysis temperature is 35-40 ℃, and the enzymolysis time is 3h.
4. The process for producing rhizoma gastrodiae decoction pieces rich in gastrodin according to claim 1, wherein in the step A3, the volume concentration of the ethanol solution is 70%, and the volume ratio of the ethanol solution to the mother liquor is 1.1-1.25.
5. The process for producing rhizoma Gastrodiae decoction pieces rich in gastrodin according to claim 1, wherein in the step S2, the drying temperature of the drying treatment is 80-90 deg.C, and hot air drying is adopted.
6. The process for producing rhizoma Gastrodiae decoction pieces rich in gastrodin as claimed in claim 1, wherein in step S3, the temperature of the secondary baking is 40-50 ℃.
7. A gastrodia elata decoction piece rich in gastrodin, which is characterized by being prepared by the production process as claimed in any one of claims 1 to 6.
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| CN101531689A (en) * | 2009-04-20 | 2009-09-16 | 陕西省科学院酶工程研究所 | Method for extracting gastrodin by biological enzyme method |
| CN102579845A (en) * | 2012-03-07 | 2012-07-18 | 玉溪市维和生物技术有限责任公司 | Preparation process of tall gastrodia tuber tablet rich in gastrodin |
| CN104815191A (en) * | 2015-03-30 | 2015-08-05 | 亳州市盛林中药饮片有限公司 | Processing method of rhizoma gastrodiae decoction pieces |
| CN105477382A (en) * | 2015-12-26 | 2016-04-13 | 蔡丝英 | Processing method for gastrodia elata decoction pieces |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR100882325B1 (en) * | 2007-06-19 | 2009-02-11 | 무주군약초영농조합법인 | Method of manufacturing for enhancement gastrodin which has anti-convulsive effect |
| CN107802746A (en) * | 2017-11-13 | 2018-03-16 | 贵州景诚制药有限公司 | Rhizoma gastrodiae wall-broken decoction pieces and preparation method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101531689A (en) * | 2009-04-20 | 2009-09-16 | 陕西省科学院酶工程研究所 | Method for extracting gastrodin by biological enzyme method |
| CN102579845A (en) * | 2012-03-07 | 2012-07-18 | 玉溪市维和生物技术有限责任公司 | Preparation process of tall gastrodia tuber tablet rich in gastrodin |
| CN104815191A (en) * | 2015-03-30 | 2015-08-05 | 亳州市盛林中药饮片有限公司 | Processing method of rhizoma gastrodiae decoction pieces |
| CN105477382A (en) * | 2015-12-26 | 2016-04-13 | 蔡丝英 | Processing method for gastrodia elata decoction pieces |
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