CN114292889B - Preparation method and application of amorpha fruticosa flower polysaccharide - Google Patents
Preparation method and application of amorpha fruticosa flower polysaccharide Download PDFInfo
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- CN114292889B CN114292889B CN202111496803.7A CN202111496803A CN114292889B CN 114292889 B CN114292889 B CN 114292889B CN 202111496803 A CN202111496803 A CN 202111496803A CN 114292889 B CN114292889 B CN 114292889B
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- 240000002066 Amorpha fruticosa Species 0.000 title claims abstract description 92
- 235000004047 Amorpha fruticosa Nutrition 0.000 title claims abstract description 92
- 150000004676 glycans Chemical class 0.000 title claims abstract description 80
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 80
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 80
- 238000002360 preparation method Methods 0.000 title claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 16
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- 238000005303 weighing Methods 0.000 claims abstract description 11
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- 239000000843 powder Substances 0.000 claims abstract description 8
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- 230000001105 regulatory effect Effects 0.000 claims abstract description 4
- 238000002390 rotary evaporation Methods 0.000 claims abstract description 4
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- 239000006228 supernatant Substances 0.000 claims abstract description 4
- 230000000694 effects Effects 0.000 claims description 16
- 239000000047 product Substances 0.000 claims description 16
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- 108060008724 Tyrosinase Proteins 0.000 claims description 14
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 claims description 12
- 230000005764 inhibitory process Effects 0.000 claims description 11
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- 238000002835 absorbance Methods 0.000 description 8
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- 229940095259 butylated hydroxytoluene Drugs 0.000 description 6
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- WZNJWVWKTVETCG-UHFFFAOYSA-N kojic acid Natural products OC(=O)C(N)CN1C=CC(=O)C(O)=C1 WZNJWVWKTVETCG-UHFFFAOYSA-N 0.000 description 3
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- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 2
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- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
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- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- 206010007247 Carbuncle Diseases 0.000 description 1
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- 230000002292 Radical scavenging effect Effects 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
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- 239000011648 beta-carotene Substances 0.000 description 1
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 description 1
- 229960002747 betacarotene Drugs 0.000 description 1
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- 238000004061 bleaching Methods 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
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- HEILIGJNYTWOHU-UHFFFAOYSA-N ethanol 2-hydroxybenzoic acid Chemical compound CCO.OC(=O)C1=CC=CC=C1O HEILIGJNYTWOHU-UHFFFAOYSA-N 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
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- 210000004185 liver Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 239000010687 lubricating oil Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
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- 239000003921 oil Substances 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 238000011160 research Methods 0.000 description 1
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- 239000007921 spray Substances 0.000 description 1
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- 235000018553 tannin Nutrition 0.000 description 1
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- 238000009210 therapy by ultrasound Methods 0.000 description 1
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- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 description 1
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses polysaccharide of amorpha fruticosa and application thereof, wherein the polysaccharide of amorpha fruticosa is prepared by the following steps of weighing 50g of dry powder of amorpha fruticosa in a 1000mL round bottom flask, adding 500mL of 95% ethanol, refluxing for 3 hours, filtering, and drying filter residues to obtain defatted amorpha fruticosa precipitate for later use; weighing 2g of defatted amorpha fruticosa dry powder obtained in the step S1, adding cellulase accounting for 4% of the mass of the amorpha fruticosa, adding distilled water according to the feed-liquid ratio of 1:20 (g/ml), regulating the pH to 7, stirring uniformly, extracting for 3h at 50 ℃, inactivating enzyme for 10min at 90 ℃, centrifuging the solution after enzymolysis in a centrifuge at the speed of 5000r/min for 10min, and obtaining supernatant as an extracting solution of amorpha fruticosa polysaccharide; concentrating the extractive solution by rotary evaporation to a proper volume, adding 4 times of 95% ethanol, standing overnight at 4deg.C, centrifuging at 4000r/min for 15min, collecting precipitate, and drying at low temperature to obtain crude polysaccharide.
Description
Technical Field
The invention belongs to the technical field of skin care products, and particularly relates to a preparation method and application of amorpha fruticosa polysaccharide.
Background
Amorpha fruticosa (Amorpha fruticosa) is a perennial deciduous shrub of the family Leguminosae (Leguminosae), and is also known as: cotton locust, pepper strips, cotton sliver, and acacia. Flowers, clearing heat, cooling blood and stopping bleeding; the leaves are slightly bitter and cool, have the efficacy of eliminating dampness and detumescence, and are mainly used for treating carbuncle, eczema and burns and scalds. Northeast and southeast of the original united states are now widely cultivated in the south of the river. The branches and leaves are used as green manure and livestock feed; the stem skin can extract tannin extract, and the branches weave basket; the fruit contains aromatic oil, and the seed contains 10% oil, and can be used as raw material of paint, glycerol and lubricating oil. Is planted on river bank, river levee, sand land, hillside and railway, and has effects of protecting levee, preventing sand, preventing wind and fixing sand. In recent years, research on chemical components and pharmacological activity of amorpha fruticosa is gradually in progress, and a plurality of active components are discovered, so that the amorpha fruticosa has potential medicinal values in the aspects of resisting tumors, resisting viruses, protecting livers, killing insects and the like.
With the development of economy and the improvement of life quality of people, the demand of consumers for skin care products is increased, and the demand level of the products is also increased. The raw materials of the existing skin care products are mainly synthesized by manpower, and have certain side effects. Therefore, natural skin care products ingredients which are nontoxic, pollution-free and have no side effects are increasingly favored by consumers. The amorpha fruticosa is widely planted in China, has high yield and low price, and is an excellent polysaccharide extraction material. If the amorpha fruticosa polysaccharide can be utilized in the field of skin care products, the added value of the product can be greatly improved, and the economic benefit is increased. At present, no report on oxidation resistance and skin care function of amorpha fruticosa polysaccharide is known.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a cool insect-preventing spray and a preparation method thereof, and the effects of relieving, diminishing inflammation, resisting bacteria and protecting skin can be realized through the formula of the invention.
In order to achieve the above purpose, the invention adopts the following technical scheme,
a polysaccharide of amorpha fruticosa, the preparation of which is obtained by the following method:
s1, weighing 50g of dry powder of amorpha fruticosa, adding 500mL of 95% ethanol into a 1000mL round bottom flask, refluxing for 3 hours, filtering, and drying filter residues to obtain defatted amorpha fruticosa precipitate for later use;
s2, weighing 2g of defatted amorpha fruticosa dry powder obtained in the step S1, adding cellulase accounting for 4% of the mass of the amorpha fruticosa, adding distilled water according to the feed-liquid ratio of 1:20 (g/ml), regulating the pH to 7, stirring uniformly, extracting for 3h at 50 ℃, inactivating enzyme at 90 ℃ for 10min, centrifuging the solution after enzymolysis in a centrifuge at the rotating speed of 5000r/min for 10min, and obtaining supernatant as an extracting solution of amorpha fruticosa polysaccharide;
s3, concentrating the extracting solution by rotary evaporation to a proper volume, adding 4 times of 95% ethanol, standing overnight at 4 ℃, centrifuging at 4000r/min for 15min, taking precipitate, and drying at low temperature to obtain crude polysaccharide.
The invention provides an application of amorpha fruticosa polysaccharide, which is used for eliminating DPPH free radicals.
The invention also provides an application of the amorpha fruticosa polysaccharide, wherein the amorpha fruticosa polysaccharide is used for removing OH free radicals.
The invention further provides an application of the amorpha fruticosa polysaccharide, wherein the amorpha fruticosa polysaccharide is used for resisting oxidation of a beta-carotene-linoleic acid system.
The invention further provides an application of the amorpha fruticosa polysaccharide, wherein the amorpha fruticosa polysaccharide is used for inhibiting the activity of tyrosinase.
The invention further provides application of the amorpha fruticosa polysaccharide, and application of the amorpha fruticosa polysaccharide in moisture absorption and moisture preservation.
Secondly, the invention further provides a skin care product containing the amorpha fruticosa polysaccharide, wherein the amorpha fruticosa polysaccharide can be used as an additive component in the skin care product, and the content of the amorpha fruticosa polysaccharide is 1-10%.
The skin care product can have single DPPH free radical scavenging or OH free radical scavenging or beta-carotene-linoleic acid system antioxidant or tyrosinase activity inhibiting effect or moisture absorption and retention effect or a plurality of combinations thereof.
The amorpha fruticosa polysaccharide has the advantages of high antioxidant activity, inhibition of tyrosinase synthesis, blocking of melanin generation, good moisture absorption and preservation activity, good effects of supplementing skin moisture, whitening skin, resisting skin aging and the like, and can be used for developing natural antioxidants and cosmetic additives.
Drawings
FIG. 1 is a graph showing the DPPH radical scavenging ability of amorpha fruticosa polysaccharide in example 1 of the present invention;
FIG. 2 is a graph showing the OH free radical scavenging ability of amorpha fruticosa polysaccharide according to example 2 of the present invention;
FIG. 3 is a graph showing the inhibition of the beta-carotene-linoleic acid oxidation system by amorpha fruticosa polysaccharide and BHT in example 3 of the present invention;
FIG. 4 is a graph showing the inhibitory activity of amorpha fruticosa polysaccharide and kojic acid on tyrosinase in example 4 of the present invention;
FIG. 5 is a graph showing the data of moisture absorption rate of amorpha fruticosa polysaccharide in example 5 of the present invention;
FIG. 6 is a graph showing the data of moisture retention rate of amorpha fruticosa polysaccharide in example 5 of the present invention.
Detailed Description
The following description of the present invention will further illustrate the present invention, and the following examples are provided on the premise of the present technical solution, and the detailed implementation and the specific operation procedure are given, but the protection scope of the present invention is not limited to the present examples.
The invention relates to polysaccharide of amorpha fruticosa, which is prepared by the following steps:
s1, weighing 50g of dry powder of amorpha fruticosa, adding 500mL of 95% ethanol into a 1000mL round bottom flask, refluxing for 3 hours, filtering, and drying filter residues to obtain defatted amorpha fruticosa precipitate for later use;
s2, weighing 2g of defatted amorpha fruticosa dry powder obtained in the step S1, adding cellulase accounting for 4% of the mass of the amorpha fruticosa, adding distilled water according to the feed-liquid ratio of 1:20 (g/ml), regulating the pH to 7, stirring uniformly, extracting for 3h at 50 ℃, inactivating enzyme at 90 ℃ for 10min, centrifuging the solution after enzymolysis in a centrifuge at the rotating speed of 5000r/min for 10min, and obtaining supernatant as an extracting solution of amorpha fruticosa polysaccharide;
s3, concentrating the extracting solution by rotary evaporation to a proper volume, adding 4 times of 95% ethanol, standing overnight at 4 ℃, centrifuging at 4000r/min for 15min, taking precipitate, and drying at low temperature to obtain crude polysaccharide.
The invention provides an application of amorpha fruticosa polysaccharide, which is used for eliminating DPPH free radicals.
The invention also provides an application of the amorpha fruticosa polysaccharide, wherein the amorpha fruticosa polysaccharide is used for removing OH free radicals.
The invention further provides an application of the amorpha fruticosa polysaccharide, wherein the amorpha fruticosa polysaccharide is used for resisting oxidation of a beta-carotene-linoleic acid system.
The invention further provides an application of the amorpha fruticosa polysaccharide, wherein the amorpha fruticosa polysaccharide is used for inhibiting the activity of tyrosinase.
The invention further provides application of the amorpha fruticosa polysaccharide, and application of the amorpha fruticosa polysaccharide in moisture absorption and moisture preservation.
Secondly, the invention further provides a skin care product containing the amorpha fruticosa polysaccharide, wherein the amorpha fruticosa polysaccharide can be used as an additive component in the skin care product, and the content of the amorpha fruticosa polysaccharide is 1-10%.
The skin care product can have single DPPH free radical scavenging or OH free radical scavenging or beta-carotene-linoleic acid system antioxidant or tyrosinase activity inhibiting effect or moisture absorption and retention effect or a plurality of combinations thereof.
Example 1
Scavenging activity of amorpha fruticosa polysaccharide on DPPH
1. Preparation of DPPH test solution: accurately weighing 8.0mg of DPPH, dissolving with 100mL of methanol to prepare 0.08mg/mL of DPPH solution, and preserving in dark for later use.
2. The experimental steps are as follows: preparing amorpha fruticosa polysaccharide prepared in example 1 and a positive drug Butylated Hydroxytoluene (BHT) into five series concentration test solutions of 1mg/mL, 0.5mg/mL, 0.25mg/mL, 0.125mg/mL and 0.06mg/mL, and mixing 2.0mL series concentration amorpha fruticosa polysaccharide solution and 1.0mL DPPH solution to obtain a solution s; mixing 2.0mL of polysaccharide solution with serial concentration and 1.0mL of methanol to obtain solution b; a mixture of 2.0mL of methanol and 1.0mL of DPPH solution was taken and designated as solution c. The above solutions were kept away from light at room temperature and placed in a shaker for 30min, absorbance values As, ab, ac were measured at 517nm, 3 multiplex wells were set per group, and 3 replicates were measured. According to the formula: clearance (%) = [1- (As-Ab)/Ac ] ×100% clearance to DPPH radical was calculated, and the result is shown in fig. 1. The clearance rate of the amorpha fruticosa polysaccharide (number 1 in the figure) on DPPH is close to that of the positive drug BHT (number 2 in the figure) at the concentration of 1 mg/mL.
Example 2
Scavenging activity of amorpha fruticosa polysaccharide on OH
The amorpha polysaccharide prepared in example 1 and the positive drug Vc were formulated into a sample solution of 2.0 mg/mL. Taking 5-branch test tubes with stopper, adding 2.0mmol/L FeSO to each of the test tubes 4 1.0mL, 6.0mmol/L salicylic acid-ethanol solution 1.0mL, shaking, adding polysaccharide solution 0, 0.2, 0.4, 0.6, 0.8, 1.0mL, distilled water 1, 0.8, 0.6, 0.4, 0.2, 0.0mL, respectively, 1.0mmol/L H, respectively 2 O 2 1.0mL, shaking, heating in 37deg.C water bath for 30min, zeroing with distilled water, and measuring absorbance at 510nm with ultraviolet spectrophotometer. The clearance of OH was calculated As follows, clearance (%) = [1- (As-Ab)/Ac ]]X 100%, where A c To add 1mL of distilled water, A s To add absorbance values of samples of different concentrations, A b To add no H 2 O 2 The results are shown in FIG. 2. The clearance rate of the amorpha fruticosa polysaccharide (number 1 in the figure) on OH at the concentration of 1mg/mLSimilar to the positive drug Vc (number 2 in the figure).
From the results, the amorpha fruticosa polysaccharide has strong antioxidant activity, and can be further developed into natural antioxidants.
Example 3
Antioxidation effect of amorpha fruticosa polysaccharide on beta-carotene-linoleic acid system
Precisely sucking 3ml of beta-carotene-chloroform solution with the concentration of 0.1mg/ml, adding 400 mu l of Tween 40 and 40 mu l of linoleic acid, shaking uniformly, rotationally evaporating at 50 ℃ for 5min, adding 200ml of distilled water, and carrying out ultrasonic treatment for 20min to obtain a reaction solution A. 5ml of the reaction solution A was taken, 100ul of 2mg/ml of the amorpha fruticosa polysaccharide solution was added, and the mixture was heated in a water bath at 50℃for 3 hours, and the absorbance thereof was measured at 470nm every 20 minutes. BHT was used as a positive control. Inhibition of beta-carotene bleaching (I%) can be calculated by the following formula:
I％＝(1-Rs/Rc)×100％
r (degradation rate) =ln (a/b) × (1/t), where a is the absorbance at t=0 and b is the absorbance at t= (20 min,40min,60min … …).
The results are shown in FIG. 3. The results show that the inhibition capacity of the amorpha fruticosa polysaccharide (No. 1 in the figure) on the beta-carotene-linoleic acid oxidation system is about 83% of that of BHT (No. 2 in the figure), which shows that the amorpha fruticosa polysaccharide has better inhibition capacity on lipid oxidation.
Example 4
Inhibition activity of amorpha fruticosa polysaccharide on tyrosinase
The amorpha fruticosa polysaccharide prepared in the example 1 and the positive medicine kojic acid are respectively prepared into sample solutions with different concentrations, the reaction is carried out in a 96-well culture plate, and the total reaction system is 200 mu L: 40 mu L of sample solutions with different concentrations; 67mmol/L phosphate buffer 80. Mu.L; 40. Mu.L of 100U/mL tyrosinase solution; 2.5mmol/L L-tyrosine 40. Mu.L. Only 67mmol/L of phosphate buffer 200. Mu.L was added to the zeroed wells. After the sample is added, the mixture is evenly mixed, the mixture is placed in a 37 ℃ water bath incubator for 20min, the enzyme-linked immunosorbent assay instrument is used for detecting the specific absorbance value of the reaction product at the wavelength of 490nm, and the test is repeated for 3 times.
The sample and kojic acid pair tyrosinase were calculated according to the following formulaIs effective in inhibiting the inhibition of the action of (a). Inhibition rate (I%) = [1- (a) 1 -A 2 )/(A 3 -A 4 )]X 100%, wherein: a is that 1 、A 2 、A 3 、A 4 The absorbance of the sample group, the sample background group (PBS instead of tyrosinase), the blank group (distilled water instead of sample), and the blank background group (distilled water instead of sample, PBS instead of tyrosinase), respectively, are shown in fig. 4. The result shows that the inhibition rate of the amorpha fruticosa polysaccharide to tyrosinase can reach 56.32%, and the amorpha fruticosa polysaccharide has a good inhibition effect on tyrosinase.
Example 5
Moisture absorption and retention activity of amorpha fruticosa polysaccharide
1. Determination of hygroscopicity of amorpha fruticosa polysaccharide
The amorpha fruticosa polysaccharide is dried in an oven at 55 ℃ for 4 hours. At a constant temperature of 20 ℃, a saturated aqueous potassium sulfate solution in a dryer is used for maintaining RH at 86.5%, 100mg of samples are accurately weighed and placed in weighing bottles of 25X 25mm, three parallel samples are taken each, glycerin is taken as a control group, and the samples are placed in the dryer. After a certain time interval, each sample is accurately weighed until 72 hours later and taken out.
Moisture absorption (%) = (m) n -m 0 )/m 0 ×100％
Wherein: m is m 0 For the initial mass of the sample, m n For sample mass after n hours of standing. The results are shown in FIG. 5. The results indicate that the amorpha polysaccharide (number 1 in the figure) has better hygroscopicity than glycerol (number 2 in the figure).
2. Moisture retention determination of amorpha fruticosa polysaccharide
The amorpha fruticosa polysaccharide is dried in an oven at 55 ℃ for 4 hours. At a constant temperature of 20 ℃, the RH was maintained at 32% with a saturated aqueous solution of calcium chloride in a desiccator. 100mg of the sample was accurately weighed, placed in weighing bottles of 25X 25mm, and 40% by mass of water was added to each bottle, three replicates each with glycerin as a control, and placed in a desiccator. After a certain time interval, each sample is accurately weighed until 72 hours later and taken out.
Moisture retention (%) =m n /m 0
In the middle of：m 0 For the initial water mass of the sample, m n The water content of the sample after n hours of standing is measured. The results are shown in FIG. 6. The results indicate that the amorpha fruticosa polysaccharide (number 1 in the figure) has better moisture retention than glycerol (number 2 in the figure).
Various modifications and variations of the present invention will be apparent to those skilled in the art in light of the foregoing teachings and are intended to be included within the scope of the following claims.
Claims (3)
1. A polysaccharide of amorpha fruticosa, characterized in that the preparation of the polysaccharide of amorpha fruticosa is obtained by the following method:
s1, weighing 50g of dry powder of amorpha fruticosa, adding 500mL of 95% ethanol into a 1000mL round bottom flask, refluxing for 3 hours, filtering, and drying filter residues to obtain defatted amorpha fruticosa precipitate for later use;
s2, weighing 2g of defatted amorpha fruticosa dry powder obtained in the step S1, adding cellulase accounting for 4% of the mass of the amorpha fruticosa, adding distilled water according to the feed-liquid ratio of 1:20 (g/ml), regulating the pH to 7, stirring uniformly, extracting for 3h at 50 ℃, inactivating enzyme at 90 ℃ for 10min, centrifuging the solution after enzymolysis in a centrifuge at the rotating speed of 5000r/min for 10min, and obtaining supernatant as an extracting solution of amorpha fruticosa polysaccharide;
s3, concentrating the extracting solution by rotary evaporation to a proper volume, adding 4 times of 95% ethanol, standing overnight at 4 ℃, centrifuging at 4000r/min for 15min, taking precipitate, and drying at low temperature to obtain crude polysaccharide.
2. Use of the amorpha fruticosa polysaccharide according to claim 1, for the preparation of a skin care product, wherein the amorpha fruticosa polysaccharide is used as an additive in skin care products for the scavenging of DPPH free radicals or for scavenging of OH free radicals or for antioxidation of the beta-carotene-linoleic acid system or for inhibition of tyrosinase activity.
3. The use of amorpha fruticosa polysaccharide according to claim 2, characterized in that the amorpha fruticosa polysaccharide is present in an amount of 1% -10% as an additive in skin care products.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1980000566A1 (en) * | 1978-09-12 | 1980-04-03 | Bristol Myers Co | N-substituted-6-oxamorphinans |
| GB9522176D0 (en) * | 1995-10-30 | 1996-01-03 | Iaf Biochem Int | Morphinan derivatives having neuroprotective activity |
| CN103421062A (en) * | 2013-08-30 | 2013-12-04 | 南京泽朗医药科技有限公司 | Preparation method for amorpha fruticosa glycosides |
| WO2015192053A1 (en) * | 2014-06-13 | 2015-12-17 | Purdue Pharma L.P. | Azamophinan derivatives and use thereof |
| CN108822227A (en) * | 2018-06-14 | 2018-11-16 | 林仁周 | A kind of polysaccharide and the application in terms of CIK cell culture |
-
2022
- 2022-03-14 CN CN202111496803.7A patent/CN114292889B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1980000566A1 (en) * | 1978-09-12 | 1980-04-03 | Bristol Myers Co | N-substituted-6-oxamorphinans |
| GB9522176D0 (en) * | 1995-10-30 | 1996-01-03 | Iaf Biochem Int | Morphinan derivatives having neuroprotective activity |
| CN103421062A (en) * | 2013-08-30 | 2013-12-04 | 南京泽朗医药科技有限公司 | Preparation method for amorpha fruticosa glycosides |
| WO2015192053A1 (en) * | 2014-06-13 | 2015-12-17 | Purdue Pharma L.P. | Azamophinan derivatives and use thereof |
| CN108822227A (en) * | 2018-06-14 | 2018-11-16 | 林仁周 | A kind of polysaccharide and the application in terms of CIK cell culture |
Non-Patent Citations (4)
| Title |
|---|
| Antimicrobial flavonoids as a potential substitute for overcoming antimicrobial resistance;Avadh Biharee等;《Fitoterapia》;104720 * |
| Antioxidant Activity of the Extracts from Amorpha Fruticosa Bark;LEE Hyun-Jung等;《춘계총회 및 학술대회》;第298页 * |
| 紫穗槐化学成分及药理活性研究进展;赵昱玮;南敏伦;赫玉芳;吕娜;王文君;李清梅;赵全成;王艳芳;;中国实验方剂学杂志(01);全文 * |
| 紫穗槐花总黄酮提取工艺的优化;黎代余 等;《贵州农业科学》;第136-139页 * |
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