CN114287621A - Composition with physical fatigue relieving effect - Google Patents
Composition with physical fatigue relieving effect Download PDFInfo
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- CN114287621A CN114287621A CN202111626474.3A CN202111626474A CN114287621A CN 114287621 A CN114287621 A CN 114287621A CN 202111626474 A CN202111626474 A CN 202111626474A CN 114287621 A CN114287621 A CN 114287621A
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Abstract
The invention discloses a composition with effect, which comprises 1-12 parts of angelica, 1-30 parts of astragalus, 1-9 parts of pseudo-ginseng, 1-10 parts of epimedium and 1-30 parts of codonopsis pilosula. The composition can relieve physical fatigue, thereby promoting health.
Description
Technical Field
The invention belongs to the technical field of food, and particularly relates to a composition with a function of relieving physical fatigue.
Background
With the acceleration of life rhythm, fatigue has become an important factor influencing the quality of life of people, and is a nonspecific and multifaceted symptom comprising complex interaction among biological, psychological, social and behavioral processes. Fatigue can cause a series of physiological changes of the body, such as sleep disorder, endocrine disorder, immune dysfunction, metabolic disorder and the like. Chronic fatigue, if not relieved in time, can lead to many diseases such as aging, depression, cancer, multiple sclerosis and parkinson's disease. According to data statistics, 70 percent of the adults in the world are in a sub-health state, wherein the symptoms of physical and mental fatigue account for more than 80 percent. In the future, the health care product for relieving physical fatigue becomes a pet in the market.
At present, most of the products for relieving physical fatigue in the market are prepared by combining extracts, and the traditional Chinese medicine is pure natural and has long-term effect, so that the invention of the functional food which takes the traditional Chinese medicine as the raw material and has the function of relieving the physical fatigue has great significance.
Disclosure of Invention
The invention aims to provide a composition with the effect of relieving physical fatigue, and the composition has the advantages that various components are reasonably combined and used together, and the health-care effect of relieving physical fatigue is achieved through multiple ways and multiple layers.
The composition with the effect of relieving physical fatigue comprises the components of Chinese angelica, astragalus, pseudo-ginseng, epimedium and codonopsis pilosula.
The weight ratio of each component in the composition of the invention is as follows: 1-12 parts of angelica, 1-30 parts of astragalus, 1-9 parts of pseudo-ginseng, 1-10 parts of epimedium and 1-30 parts of codonopsis pilosula.
The preferred weight ratio of each component in the composition of the invention is as follows: 1-6 parts of angelica, 1-9 parts of astragalus, 1-3 parts of pseudo-ginseng, 1-6 parts of epimedium and 1-9 parts of codonopsis pilosula.
The optimal weight ratio of each component in the composition is as follows: 5 parts of angelica, 8 parts of astragalus, 3 parts of pseudo-ginseng, 5 parts of epimedium and 8 parts of codonopsis pilosula.
The composition with effect of relieving physical fatigue can be made into tablet, oral liquid, granule, unguent, tablet candy, biscuit, beverage, etc.
The above components radix Angelicae sinensis has effects in dilating blood vessel, promoting differentiation of hematopoietic cells and hematopoietic stem cells, improving microcirculation, relieving pain, relieving convulsion, and enhancing immunity. Modern researches show that the high-dose Chinese angelica water decoction can obviously prolong the swimming time of blood-deficiency mice, reduce the accumulation of lactic acid in the bodies of the mice after exercise, obviously reduce the BUN content and improve the anti-fatigue capability of the blood-deficiency mice. Radix astragali has effects of tonifying heart, regulating blood pressure, protecting cerebral ischemia reperfusion injury, improving cerebral degenerative disease, and promoting cellular immunity. Modern studies have shown that astragalus polysaccharides and astragaloside can relieve peripheral fatigue by increasing the reserve of energy substances, reducing metabolite accumulation, improving TCA cycle and mitochondrial dysfunction, increasing the sensitivity of muscle response and inhibiting oxidative stress. Radix Codonopsis has effects of regulating gastrointestinal motility, resisting ulcer, resisting anoxia, and delaying aging. Modern researches show that codonopsis pilosula can reduce accumulation of metabolites and increase glycogen content by increasing expression of PGC-1 alpha protein, so that the effect of resisting exercise-induced fatigue is achieved. Notoginseng radix has effects of dilating blood vessel, reducing oxygen consumption of myocardium, inhibiting platelet aggregation, scavenging free radicals, and resisting oxidation. Ginsenoside Rg as effective component in Notoginseng radix1Can improve liver glycogen content in mice after exercise, thereby improving endurance capacity and exercise capacity of the mice and resisting fatigue. Herba Epimedii has effects of enhancing specific and nonspecific immunity, resisting arrhythmia, lowering demand, and enhancing reproductive system function. Researches show that the epimedium can improve hemoglobin, blood sugar and blood lactic acid levels of athletes to different degrees, improve organism metabolism, is beneficial to maintaining high-strength exercise capacity, and particularly has special significance on the anti-fatigue effect of long-time endurance projects.
Detailed Description
The invention will now be further illustrated by reference to the following examples, which are given by way of illustration only.
Example 1:
the composition comprises the following components in parts by weight: 1 part of angelica, 1 part of astragalus, 1 part of pseudo-ginseng, 1 part of epimedium and 1 part of codonopsis pilosula.
Example 2:
the composition comprises the following components in parts by weight: 5 parts of angelica, 8 parts of astragalus, 3 parts of pseudo-ginseng, 5 parts of epimedium and 8 parts of codonopsis pilosula.
Example 3:
the composition comprises the following components in parts by weight: 6 parts of angelica, 9 parts of astragalus, 3 parts of pseudo-ginseng, 6 parts of epimedium and 9 parts of codonopsis pilosula.
Example 4:
the composition comprises the following components in parts by weight: 12 parts of angelica, 10 parts of astragalus, 9 parts of pseudo-ginseng, 10 parts of epimedium and 15 parts of codonopsis pilosula.
Example 5:
the composition comprises the following components in parts by weight: 12 parts of angelica, 30 parts of astragalus, 9 parts of pseudo-ginseng, 10 parts of epimedium and 30 parts of codonopsis pilosula.
Example 6: influence on the alleviation of physical fatigue of mice
6.1 Experimental materials
The epimedium composition is granules prepared according to the mixture ratio of the embodiment 3; the preparation method comprises the steps of weighing 6 parts of angelica sinensis, 9 parts of astragalus membranaceus, 3 parts of pseudo-ginseng, 6 parts of epimedium herb and 9 parts of codonopsis pilosula respectively, mixing the raw materials, adding the raw materials into an extraction tank, boiling and decocting for three times, adding 8 times of water of the total weight of the raw materials each time, extracting, filtering, mixing extraction filtrate obtained in three times, concentrating, drying, powdering, sieving, adding 1 time of soluble starch, mixing uniformly, performing dry granulation, and sieving to obtain the test article particles.
Preparing a control granule 1 by the same method, weighing 20 parts of angelica, 15 parts of poria cocos, 10 parts of astragalus membranaceus, 15 parts of epimedium, 10 parts of pseudo-ginseng, 15 parts of codonopsis pilosula, 3 parts of red paeony root, 10 parts of salvia miltiorrhiza, 10 parts of safflower and 15 parts of raw pollen typhae respectively, grinding the raw materials into powder, wrapping the powder with gauze, soaking the powder in kaoliang wine for 6 days, stirring the powder for 3min at 12 hours and 24 hours respectively, centrifuging the powder for 5min at 3000r/min, taking supernatant, filtering the supernatant, collecting filtrate, concentrating, drying, powdering, sieving, adding 1 time of soluble starch, uniformly mixing, granulating by a dry method, and sieving to obtain the control granule 1.
Preparing a control granule 2 by the same method, weighing 9 parts of astragalus, 3 parts of pseudo-ginseng, 6 parts of epimedium and 9 parts of codonopsis pilosula respectively, mixing the raw materials, adding the mixture into an extraction tank, boiling and decocting for three times, adding 8 times of water of the total weight of the raw materials each time, extracting, filtering, mixing the three extraction filtrates, concentrating, drying, pulverizing into powder, sieving, adding 1 time of soluble starch, mixing uniformly, performing dry granulation, and sieving to obtain the control granule 2.
Preparing a control granule 3 by the same method, weighing 6 parts of angelica, 3 parts of pseudo-ginseng, 6 parts of epimedium and 9 parts of codonopsis pilosula respectively, mixing the raw materials, adding the mixture into an extraction tank, boiling and decocting the mixture for three times, adding 8 times of water of the total weight of the raw materials each time, extracting, filtering, mixing the three extraction filtrates, concentrating, drying, powdering, sieving, adding 1 time of soluble starch, mixing uniformly, granulating by a dry method, and sieving to obtain the control granule 3.
Preparing a control granule 4 by the same method, weighing 6 parts of angelica, 9 parts of astragalus, 6 parts of epimedium and 9 parts of codonopsis pilosula respectively, mixing the raw materials, adding the mixture into an extraction tank, boiling and decocting for three times, adding 8 times of water of the total weight of the raw materials each time, extracting, filtering, mixing the three extraction filtrates, concentrating, drying, powdering, sieving, adding 1 time of soluble starch, mixing uniformly, granulating by a dry method, and sieving to obtain the control granule 4.
Preparing a control granule 5 by the same method, weighing 6 parts of angelica, 9 parts of astragalus, 3 parts of pseudo-ginseng and 9 parts of codonopsis pilosula respectively, mixing the raw materials, adding the mixture into an extraction tank, boiling and decocting the mixture for three times, adding 8 times of water of the total weight of the raw materials each time, extracting, filtering, mixing the three extraction filtrates, concentrating, drying, powdering, sieving, adding 1 time of soluble starch, uniformly mixing, granulating by a dry method, and sieving to obtain the control granule 5.
Preparing a control granule 6 by the same method, weighing 6 parts of angelica, 9 parts of astragalus, 3 parts of pseudo-ginseng and 6 parts of epimedium respectively, mixing the raw materials, adding the mixture into an extraction tank, boiling and decocting for three times, adding 8 times of water of the total weight of the raw materials each time, extracting, filtering, mixing the three extraction filtrates, concentrating, drying, powdering, sieving, adding 1 time of soluble starch, mixing uniformly, granulating by a dry method, and sieving to obtain the control granule 6.
Blood UREA (UREA) determination kit (Shenzhen Meirui biomedical electronics, Inc.), liver glycogen determination kit (Shenzhen Meirui biomedical electronics, Inc.), and whole blood lactic acid determination kit (Nanjing institute of bioengineering).
130 SPF male KM mice weigh 18-22 g. The light and the shade are alternated after 12 hours of illumination, the temperature is 20-26 ℃, the relative humidity is 40% -70%, and the experiment is started after 3 days of adaptive feeding.
6.2 animal groups
Male KM mice were randomly divided into 13 groups (blank control group, low dose test group, medium dose test group, high dose test group, control granule group 1-6 group, codonopsis pilosula tablet group, astragalus tablet group, angelica tablet group) by weight layer, 40 mice per group were subjected to load swimming, serum urea assay, liver glycogen assay, blood lactic acid assay, respectively.
The test was carried out according to the requirements of "method for testing anti-fatigue effect" in "program for evaluating health food functionality and method for testing" of testing health food "(2003 edition). 9 dose groups of 1-6 groups of high, medium, low and control granules are respectively given 1.0, 0.75 and 0.5 g/kg-1、0.5g·kg-1Based on the amount of Chinese angelica in the formula; for administration, astragalus root, angelica and codonopsis pilosula decoction pieces are decocted into corresponding single decoction by using distilled water, which is equivalent to the respective crude drug amounts: codonopsis pilosula 1.2 g/kg-1Astragalus root 1.2 g.kg-10.75 g/kg of Chinese angelica-1(ii) a The test sample groups before use were prepared with distilled water at concentrations of 0.025, 0.0375, 0.05 g/mL-1The solution (calculated by the crude drug of angelica in the prescription) is prepared into 0.06 g/mL of single decoction pieces-1、0.06g·mL-1、0.0375g·mL-1The gavage volume of each group of mice is 20mL kg-1. Mice were gavaged with equal volume of distilled water for the blank control group. The administration amount is readjusted by pressing the animal body every week for 30 days after intragastric administration for 1 time every day.
6.3 weight bearing swimming test
Refer to "evaluation procedure and test method of health food Functionality" (2003 edition) "examination method of anti-fatigue action" of Ministry of health. After 10 mice in each group were given the sample for 30min last time, the tail of each mouse was loaded with a 5% weight lead skin, placed in a tank with water depth of 40cm and water temperature of 25 ℃ for swimming, and the time from swimming to death of the mouse was recorded.
The results show that the mice in the high, medium and low doses, the 6 control granule groups and the decoction piece groups have prolonged swimming time under load compared with the blank control group, the radix codonopsis decoction piece group can remarkably prolong the swimming time of the mice (P is less than 0.05), and the high, medium and low dose groups and the control granule 1 can remarkably prolong the swimming time under load of the mice (P is less than 0.01), and the results are shown in table 1.
TABLE 1 weight bearing swimming time results for each group of mice
Note: p < 0.05, P < 0.01, compared to the blank control group.
6.4 serum Urea Nitrogen determination
10 mice in each group are respectively administered for 30min at the last time, then the mice are placed in a swimming box with the water temperature of 30 ℃ for 90min without load, and after 60min of rest, the whole blood is collected by picking eyeballs and about 0.5 mL. 2000 r.min after blood coagulation-1Centrifuging for 15min, collecting supernatant, and measuring urea nitrogen content in serum with full-automatic biochemical analyzer.
The results show that the serum urea nitrogen content of mice in the high, medium and low 3 doses, 6 control granule groups, the codonopsis pilosula tablet group and the astragalus decoction piece group is remarkably reduced (P is less than 0.05) compared with the blank control group, wherein the difference between the high, medium and low dose groups and the control granule 1 group and the blank control group is extremely remarkable (P is less than 0.01), and the results are shown in table 2. The epimedium composition can inhibit the generation of serum urea nitrogen of fatigue mice.
TABLE 2 results of serum urea nitrogen content after exercise in various groups of mice
Note: p < 0.05, P < 0.01, compared to the blank control group.
6.5 liver glycogen assay
10 mice in each group are killed by dislocation of cervical vertebra immediately after last administration for 30min, livers are taken out, the livers are placed in frozen physiological saline, connective tissues are removed, filter paper is sucked dry, the absorbance is measured according to the operation of a liver glycogen test box instruction, and the liver glycogen content is calculated.
Compared with a blank control group, the liver glycogen storage amount of mice with high, medium and low doses of 3, 6 control particle groups, codonopsis pilosula drink group and astragalus drink group is remarkably increased (P is less than 0.05), wherein the difference between the high, medium and low dose groups, the control particle group of 1, the control particle group of 2, the control particle group of 3, the control particle group of 4, the control particle group of 5 and the codonopsis pilosula drink group and the blank control group is extremely remarkable (P is less than 0.01), and the results are shown in a table 3. The epimedium composition has the function of promoting the liver glycogen storage of mice.
TABLE 3 measurement of hepatic glycogen content in each group of mice
Note: p < 0.05, P < 0.01, compared to the blank control group.
6.6 blood lactic acid assay
10 mice in each group, 30min after the last administration, respectively before and 20min after swimming, respectively taking 0.1mL of blood from orbital venous plexus of mice, operating according to the instruction of a whole blood lactic acid test box, measuring absorbance, and calculating blood lactic acid content.
Compared with a blank control group, the blood lactic acid values of mice in a high dose group, a medium dose group, a low dose group, a 6 control particle group, a radix codonopsis pilosulae group and an astragalus membranaceus decoction piece group are remarkably reduced (P is less than 0.05), wherein the difference between the high dose group, the medium dose group, the low dose group, the control particle group 1, the control particle group 2, the control particle group 3, the control particle group 4, the control particle group 5 and the radix codonopsis decoction piece group and the blank control group is extremely remarkable (P is less than 0.01), and the results are shown in a table 4. The epimedium composition has the function of reducing the generation of lactic acid after the movement of mice.
TABLE 4 blood lactate values of the groups of mice
Note: p < 0.05, P < 0.01, compared to the blank control group.
Under the experimental conditions and the designed dosage, the epimedium composition can obviously prolong the weight-bearing swimming time of the mouse; reducing the serum urea nitrogen content of the mice after swimming; increasing liver glycogen stores in the mouse; the lactic acid value of the swimming mice is obviously reduced, and in conclusion, the swimming mice have the effect of relieving physical fatigue.
Under the experimental conditions and the designed dosage, the epimedium composition can obviously prolong the weight-bearing swimming time of the mouse; reducing the serum urea nitrogen content of the mice after swimming; increasing liver glycogen stores in the mouse; the lactic acid value of the swimming mice is obviously reduced, and in conclusion, the swimming mice have the effect of relieving physical fatigue.
In the above examples, the components can be made into tablets, oral liquids, granules, ointments, pressed candies, biscuits and beverage foods by conventional processes.
The above description is only for the specific embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be made by those skilled in the art without inventive work within the technical scope of the present invention disclosed herein are intended to be covered by the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope defined by the claims.
Claims (6)
1. A composition with the effect of relieving physical fatigue is characterized in that: the composition comprises the components of angelica, astragalus, pseudo-ginseng, epimedium and codonopsis pilosula.
2. The composition with physical fatigue relieving effect according to claim 1, wherein: the composition comprises 1-12 parts of angelica, 1-30 parts of astragalus, 1-9 parts of pseudo-ginseng, 1-10 parts of epimedium and 1-30 parts of codonopsis pilosula in parts by weight.
3. The composition with physical fatigue relieving effect according to claim 1, wherein: the composition comprises 1-6 parts of angelica, 1-9 parts of astragalus, 1-3 parts of pseudo-ginseng, 1-6 parts of epimedium and 1-9 parts of codonopsis pilosula in parts by weight.
4. The composition for relieving physical fatigue according to any one of claims 1 to 3, wherein: the composition comprises 5 parts of angelica, 8 parts of astragalus, 3 parts of pseudo-ginseng, 5 parts of epimedium and 8 parts of codonopsis pilosula in parts by weight.
5. The composition for relieving physical fatigue according to any one of claims 1 to 4, wherein: the composition can be prepared into tablets, oral liquid, granules, ointment, tabletting candies, biscuits and beverage foods.
6. Composition for relieving physical fatigue effects according to any of claims 1 to 5, characterized in that: the composition is applied to preparing foods and health-care foods for relieving physical fatigue.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103505526A (en) * | 2013-08-30 | 2014-01-15 | 梁卫 | Medicine composition with functions of resisting anoxia, resisting fatigue, tonifying kidney and invigorating yang and preparation method |
| CN104547171A (en) * | 2014-12-25 | 2015-04-29 | 湖南炎帝生物工程有限公司 | Traditional Chinese medicine composition for relieving physical fatigue, and preparation method and application of traditional Chinese medicine composition |
| CN105477180A (en) * | 2015-12-09 | 2016-04-13 | 兰州大学 | Traditional Chinese medicine composition for treating chronic fatigue syndrome |
| CN106421028A (en) * | 2016-08-23 | 2017-02-22 | 华南师范大学 | Traditional Chinese medicinal composition for preventing exercise induced fatigue, and preparation method thereof |
| CN106912926A (en) * | 2017-05-11 | 2017-07-04 | 全恒太 | A kind of composition health food and its preparation technology containing pseudo-ginseng, ginseng and Shorthorned Epimedium P.E |
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- 2021-12-28 CN CN202111626474.3A patent/CN114287621A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103505526A (en) * | 2013-08-30 | 2014-01-15 | 梁卫 | Medicine composition with functions of resisting anoxia, resisting fatigue, tonifying kidney and invigorating yang and preparation method |
| CN104547171A (en) * | 2014-12-25 | 2015-04-29 | 湖南炎帝生物工程有限公司 | Traditional Chinese medicine composition for relieving physical fatigue, and preparation method and application of traditional Chinese medicine composition |
| CN105477180A (en) * | 2015-12-09 | 2016-04-13 | 兰州大学 | Traditional Chinese medicine composition for treating chronic fatigue syndrome |
| CN106421028A (en) * | 2016-08-23 | 2017-02-22 | 华南师范大学 | Traditional Chinese medicinal composition for preventing exercise induced fatigue, and preparation method thereof |
| CN106912926A (en) * | 2017-05-11 | 2017-07-04 | 全恒太 | A kind of composition health food and its preparation technology containing pseudo-ginseng, ginseng and Shorthorned Epimedium P.E |
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