CN114277168B - 一种木薯frk1类似基因及其应用 - Google Patents

Abstract

本发明提供一种木薯FRK1类似基因及其应用，所述木薯FRK1类似基因，在植物受到病原体侵染时，能够响应侵染信号并进行表达，可作为响应病原体侵染信号的标记基因，用于检测病原体对木薯的侵染情况，及时发现病害，采取相应的防治措施，对木薯抗病品种和抗病分子机理研究起到了重要的推动作用。

Description

一种木薯FRK1类似基因及其应用

技术领域

本发明涉及植物基因工程技术领域，特别涉及一种木薯FRK1类似基因及其应用。

背景技术

木薯(Manihot esculenta Crantz)是热带地区重要的粮食作物，同时它也是一种重要的、很有发展潜力的再生能源作物。病害一直是影响木薯产业健康发展的重要制约因素之一。然而，木薯抗病分子机理还不清楚，抗病相关基因资源的报道缺乏，防控技术研究基础薄弱，已不能满足不断扩大的木薯产业的发展需求。因此，对木薯抗病机理及防控技术进行研究，进而采取相应的措施以减少病害对木薯产业的影响，稳步提高木薯产量，已成为木薯产业持续健康发展亟待解决的现实问题。

病原相关分子模式(PAMP)激发的植物先天免疫反应(PTI)是植物抵御病原菌的第一道防线，是植物界中更加广泛地存在着的植物防卫机制，表现为植物对绝大多数病原微生物种类普遍存在的、高效的和持续的抗病能力。因此植物先天免疫反应成为近年来植物抗病领域的研究热点。现有研究成果主要集中在模式植物拟南芥上，热带作物广谱抗性的研究，相对于植物抗病研究中的其它领域来说，还非常薄弱。因此，开展热带作物-木薯的抗病分子机理研究有望为我国热带作物广谱抗性育种提供基因资源，相关研究成果也可供热带作物抗病分子机理的研究参考。

发明内容

鉴以此，本发明提出一种木薯FRK1类似基因及其在检测病原体侵染情况中应用。

本发明的技术方案是这样实现的：

一种木薯FRK1类似基因为MeFRK1基因。

优选的，所述MeFRK1基因的核苷酸序列如SEQ ID NO.1所示。

优选的，所述MeFRK1基因编码的蛋白的氨基酸序列如SEQ ID NO.9所示。

另一方面的，本发明还提供了所述木薯FRK1类似基因在检测病原体侵染情况中应用。

优选的，所述应用为FRK1类似基因在响应病原体侵染信号中的应用。

优选的，所述FRK1类似基因可作为响应病原体侵染信号的标记基因的标记基因。

优选的，所述检测病原体侵染情况的方法为检测样品中所述FRK1类似基因表达情况。

优选的，所述木薯FRK1类似基因在检测病原体侵染植物情况中的应用。

优选的，所述植物为木薯。

优选的，所述病原体为地毯草黄单胞木薯萎蔫致病变种(Xanthomonasaxonopodis pv.manihotis，Xam)和/或粉蚧虫。

与现有技术相比，本发明的有益效果是：

本发明提供了一种木薯FRK1类似基因及其应用，本发明所述木薯FRK1类似基因，在植物受到病原体侵染时，能够响应侵染信号并进行表达，可作为响应病原体侵染信号的标记基因，用于检测病原体对木薯的侵染情况，及时发现病害，采取相应的防治措施，对木薯抗病品种和抗病分子机理研究起到了重要的推动作用。

附图说明

图1为本发明实施例2的木薯FRK1类似基因的结构分析模式图；

图2为本发明实施例2的木薯FRK1类似基因的保守基序预测图；

图3为本发明实施例2的木薯FRK1类似基因家族染色体定位图

图4为本发明实施例3的木薯FRK1类似基因蛋白互作网络图；

图5为本发明实施例4的木薯FRK1类似基因在Xam细菌侵染木薯不同时间的表达值热图；

图6为本发明实施例4的木薯FRK1类似基因在粉蚧虫侵染木薯不同时间的表达值热图；

图7为本发明实施例5中MeFRK1基因在JA诱导下不同时间点的表达情况；

图8为本发明实施例5中MeFRK1基因在乙烯诱导下不同时间点的表达情况；

图9为本发明实施例5中MeFRK1基因在SA诱导下不同时间点的表达情况；

图10为本发明实施例5中在病原菌Xam刺激后MeFRK1基因的表达情况。

具体实施方式

为了更好理解本发明技术内容，下面提供具体实施例，对本发明做进一步的说明。

实施例1木薯FRK1类似基因的筛选

首先，在Phytozome(https://phytozome.jgi.doe.gov/)网站上下载木薯全基因组数据，运用BLASTP软件，以来自NCBI(https://www.ncbi.nlm.nih.gov/protrin/)数据库的拟南芥FRK1基因的氨基酸蛋白序列作为输入序列，以木薯全基因组氨基酸蛋白序列为数据库，e值设置为-10，对木薯FRK基因家族全部成员进行鉴定，选取e值为0的8条木薯FRK基因，按照比对结果顺序命名为MeFRK1～MeFRK8，核苷酸序列见SEQ ID NO.1～8，氨基酸序列见SEQ ID NO.9～16，具体信息见表1。

表1木薯FRK基因家族成员信息

筛选得到的8个FRK基因氨基酸长度均不相同，最长的是MeFRK8，有921个氨基酸残基，最短的是MeFRK3，有804个氨基酸残基，蛋白质序列平均长度为863aa。8个木薯FRK家族成员在木薯染色体上不均匀分布，其中，MeFRK1位于4号染色体上，MeFRK4、MeFRK5、MeFRK6、MeFRK7、MeFRK8都位于11号染色体，而MeFRK2和MeFRK3位于核骨架Scaffold上。

实施例2木薯FRK1类似基因的生物信息学分析

2.1木薯FRK1类似基因的结构分析

在Phytozome(https://phytozome.jgi.doe.gov/)网站下载木薯FRK1基因的内含子和外显子信息，使用在线网站GSDS(http://gsds.cbi.pku.edu.cn/)绘制基因模式图(图1)。

根据基因结构分析结果，8个候选基因聚为3大类。其中，MeFRK2和MeFRK6为一大类，MeFRK4单独为一大类，MeFRK1、MeFRK5、MeFRK7、MeFRK3和MeFRK8为一大类。

2.2木薯FRK1类似基因的保守基序分析

使用MEME(http://meme.nbcr.net/meme/cgi-bin/meme.cgi)预测木薯FRK1类似基因的保守基序，设置查找基序重复的数量为“any”，基序长度为50～250aa，预测基序的数量为3个，结果见图2和表2；

表2木薯FRK1类似基因的保守基序信息

表中，Motif1为酪氨酸蛋白激酶(Protein tyrosine kinase，PTK)结构域，能催化多种底物蛋白质酪氨酸残基磷酸化，在细胞生长、增殖、分化中具有重要作用。Motif2为内质网的碳水化合物结合蛋白(Carbohydrate-binding protein of the ER)结构域，Motif3为富含亮氨酸重复(Leucine Rich Repea，LRR)结构域，参与蛋白质的相互作用，是已知抗病蛋白的重要结构。

2.3木薯FRK1类似基因染色体定位分析

运用在Phytozome(https://phytozome.jgi.doe.gov/)网站上下载的木薯FRK基因家族信息，来制作染色体定位图(图3)。

从图3中可以看出，8个木薯FRK1类似基因在木薯染色体上不均匀分布，MeFRK1位于4号染色体上，MeFRK4、MeFRK5、MeFRK6、MeFRK7、MeFRK8这五个基因都位于11号染色体，图中表示位置的数字单位为Mb。

综合结构分析、保守基序分析和染色体定位等结果，确定MeFRK1基因为木薯基因组中先天免疫下游的标记基因。

实施例3木薯FRK1类似基因上游基因共表达分析

使用在线网站STRING(http://www.string-db.org/)的数据库，预测木薯FRK基因家族有类似的互作关系，预测得到的木薯FRK1类似基因蛋白互作网络如图4所示，互作蛋白的基因信息见表3。

表3木薯FRK1类似基因蛋白互作网络中基因信息

从蛋白互作网络中可以看出，木薯FRK1类似基因的互作蛋白大部分为2C类蛋白磷酸酶，表明木薯FRK1类似基因可能也参与了胁迫信号、激素信号转导等途径，为研究木薯FRK1类似基因在木薯中的作用提供了新的方向。

实施例4木薯FRK1类似基因响应病原体侵染的表达值热图

在NCBI的SRA数据库中下载来自木薯KU50品种的不同生长发育阶段的块根和叶的4个转录组数据包(SRR1299001，SRR1299002，SRR1299003，SRR1299000)，Xam细菌侵染木薯所得的3个转录组数据包(SRR1050891，SRR1050892，SRR1050893)，粉蚧虫侵染木薯所得的3个转录组原始数据(SRR2041628，SRR2043186，SRR2043192)。用Peiner et al.(2003)中的方法计算MeERF基因在组织特异性及各种逆境处理下的RPKM值。用Mev(MultiExperimentViewe)软件对数据进行处理，内置HCL程序进行层次聚类，结果用Heatmap图示(图5～6)。

结果显示，MeFRK1基因在Xam细菌和粉蚧虫侵染木薯后，均上调表达，说明MeFRK1基因能够响应不同病原体侵染信号，可作为木薯病害侵染情况检测的标记基因。

实施例5木薯FRK1类似基因的实时定量分析

使用Oligo7软件，为FRK家族8个基因设计实时定量引物，进行普通PCR实验检验其扩增特异性。

MeFRK1引物：F:TTGCACAATGGATGCAAGCC；R:AAGGTAACCAACGGTGCCAA；

MeFRK2引物：F：CACCCTTAAAACGCCGGTTG；R:CCACCGGAATAGTAGCGACC；

MeFRK3引物：F:AACTGCAGTCCAACCGATGA；R:TGGCTGCAAGTATTGGAGGG；

MeFRK4引物：F：GAAAGCTTGCCGCCAGTAAC；R:CTTGCCATGCTCAGGTCTCA；

MeFRK5引物：F：ACTTGACTACCCTCGTCGGA；R：TACTGGGTCATCGCCTGAGA；

MeFRK6引物：F：CACCCTTAAAACGCCGGTTG；R:GGAAAGGAGCGACCCAATGA；

MeFRK7引物：F:AAGGGTTCTTGGTAAGGGCG；R：ACCCCTGGACTGATGATGGA；

MeFRK8引物：F：GACCCATGTGTGCCAAGAGA；R：ACGGAGAGTGTCGGAGCTAT；

以木薯KU50品种的根部组织(生长期分别是73天、116天、143天)以及用激素水杨酸(SA)、茉莉酸(JA)、乙烯(Ethylene，ETH)处理的木薯SC8品种(华南八号)的叶片为样品，参照植物总RNA提取试剂(TIANGEN)说明书进行总RNA的分离，cDNA第一链的合成参照RevertAid First Stand cDNA synthesis(Thermo)说明书进行。Real-time PCR所用仪器为Rotor-Gene Q(QIAGEN)，试剂为SYBR Premix Ex TaqTM(TaKaRa)。根据引物Tm值按照TaKaRa试剂说明书，Real-time PCR扩增程序为：95℃30s；“95℃5s，55℃30s，72℃30s，40个循环”；72℃延伸10min。

结果采用2－△△Ct的计算方法进行相对定量，以木薯EF1a基因作为内标校正模板量，每个样品进行3次独立的生物学重复。各激素处理时间点MeFRK1基因的表达水平均与该处理0min(处理前)相比。使用SigmaPLot软件处理数据并作柱形图(图7～10)。

从图7中可以看出，在JA诱导下MeFRK1基因的表达量显著上调，说明该基因参与了的JA信号应答过程。

从图8中可以看出，在乙烯诱导下该基因在30min后的表达量显著上调，说明该基因能够响应乙烯信号。

从图9中可以看出该基因能响应SA的刺激，但是响应强度比JA低。

从图10中可以看出，FRK1图表示该基因在病原菌Xam刺激后能显著增强表达，也进一步表明该基因可以作为木薯应答病原菌尤其是是JA信号相关的转导过程的标记基因。

以上所述仅为本发明的较佳实施例而已，并不用以限制本发明，凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。

序列表

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<120> 一种木薯FRK1类似基因及其应用

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cgcccaaggg aattcaccat cagttttaac ggtgccaact atagacgtat taccctccac 840

tacttgaacc cacttaccac ttgttacgga cctcgtaaat caagagttaa tggctttgtg 900

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gaaggcaatc caagtctttg ccagacagat tcctgcgaga aaaagaagca caatgttctc 1440

cttccagttg ttatatcctt tgcaacagtt atggtgcttc tcttcctcag cagtatattt 1500

ttcttttgga gaatgaaaag acaagaggca acatcacaat ctaagaagga aggactagta 1560

atttcaacga atcggtcctt tagttattcg gagatcgtaa gtaatactaa taactttgaa 1620

accatcatcg gagaaggagg gttcggaaaa gtttactttg ggactctgaa agataacgtt 1680

caagttgctg tcaaattgct ctctcaaaat tcaaggcaag gctacaagga atttcaatct 1740

gaggcacaac tgttgatgat tgttcatcac agaaacttgg tttctctaat tggccactgt 1800

gatgaccgtc ataataaggc tctgatttat gaatacatgg ctaatggaaa tttgcgagag 1860

catttaacag agacaagtgg aagtattttg aattggaatg aaagacttca gattgcagca 1920

gatgcagcac agggactgga atatctacat aacggttgca agccaccgat aatccacagg 1980

gatttgaaga cttccaacat cttattgaat gaaaaattgc gagcaaagat atctgatttc 2040

gggctatcaa gagctttcac aaatgaaagt ggttcacata taacaactcg tcctgcaggc 2100

acaatcggct acctggatcc tcaggctcag tcatcaggaa actttaataa gaaaagcgac 2160

atatacagct ttgggataat tctacttgag ctgataactg gtcagcctgc cataagaaga 2220

gacgtcaatg gagaaattat tcgaatacaa gagtgggtta caccaataat tgagaatggc 2280

gatgtcagaa gcatagttga tcccaggcta cagggagact tcgataccaa ttctgcctgg 2340

aaagctgtgg aaatagctct ttcctgtgta ctgaatacag taactcgaag gccagacatg 2400

actgatgtat tgattgaact gaaggaatgt ttgggtatgg taacagctgt tgttggatct 2460

cagaggatgg atagaggaag gacaagatcc atcaattcac ttgaaatgag gtctttggaa 2520

atctacactg aaacggctcc cagtccaagg tag 2553

<210> 3

<211> 2414

<212> DNA/RNA

<213> Manihot esculenta Crantz

<400> 3

tggaagattt cataaacact ggaaaaaacg ggaacgtgtc tcatgaatat gattacaagg 60

atccttattt tggacatgta ttccaaagtt taagattctt tcctaaagga agaaggaatt 120

gctatacgtt gagacctgag caaggtaaaa atcaaaatta tcttatcaga gcattcttca 180

gatatggaaa ttatgactcc aacaatgaag ttccaaggtt tgagttgtat ctcggtgtta 240

atctttggac gacagtgcaa ccttcaagtc caaattcagt acgtgagatt attcattttt 300

ctttaactga taccattaag atctgcctcg taaacattgg ccatggaaca ccttttattt 360

cagcattgga attgaggcct ctcaaccctt ccatttatag aattgagtcc aaagacctat 420

atctagtgga acgatatgac cttggtaata attcgaccaa tagatatgtc aggtacaagg 480

atgatgttta tgatcggttc tggctcccat ggacgtttgt cgattgcatt tcattgaaca 540

ccacctcaaa tattgacgtc cagaatagca gcaatcccct tagaacaccc cttcaagttt 600

taagaactgc agtccaaccg atgaaaatat tacagatttc tcaaggtcca agagaattca 660

ccatcaattt taatggtgaa aactatggac cttttaccct ccaatacttg cagccactca 720

gcatttgtac tcgacctcgt aaatcaagaa ccattggata tgtttatttt tctatcgatc 780

cgactgtaca gtctcaccat ccaccaatcc tcaacgcctt ggagattttc attccgtgga 840

tggaccccgt ttcccccaca gacccagcag atgttgatgc tataatggcc atccagcaaa 900

catacaatat taataaagat gatagctggc aaggagaccc atgtatgcct aaagcttatt 960

cttggacagg cttgaattgc agctactaca ataattctcc taggatcatc tttttggacc 1020

tcagtgcaag tacattgaat ggaagcattt cttcctcatt ctccaagctc aaagcgatac 1080

agtacttaga cttatcaggc aatgaactta ctggaacagt tccagaattc ttagcacaac 1140

tgccaaattt aactgttcta aatttaaccg gaaacaagct cacaggttca gttcctcggt 1200

ctcttgtcca aaaatctaac agtggtctat tgcagctgag cttggacgga aacccaaatc 1260

tttgccacac agattcctgt gagaaacaga agcgaaattt tccacttcca atagttgtag 1320

catccgttgc aatacttacg gtgcttctca gcagtatatt tgtcttttgg agaatgaaaa 1380

gacgagaagg aacatcacaa tctaagaagg aaggattagt aaattcaaag aatcgatctt 1440

ttacttatac agatattgtc agtattcctg ataactttga aaccattatt ggagaaggag 1500

ggttcggaaa agtttacatg gggactctga aagataacac tcaagttgct gtcaaattgc 1560

tctctcaaaa ttcaaggcaa ggctataagg aatttcaagc tgaggcacaa ctgttgatga 1620

ttgttcatca cagaaacttg gtttctctaa ttggctactg tgatgaccgt cataataagg 1680

ctctgattta tgaatacaag gttaatggaa atttgcgaga gcatttatca gagacaagtg 1740

gaagtacttt gaattggaat gaaagacttc atattgcagc agatgcagca cacggactgg 1800

aatatctaca taacggttgc aagccaccca taatccacag ggatttgaag acttccaaca 1860

tcttattgaa tgaaaaattg ggagcaaaga tatctgattt cgggctatca agagctttca 1920

caaatgaaag tggttcacat ataacaactc gtcctgcagg cacactcggc tacctggatc 1980

ctcaggctca gtcatcagga aactttaata agaaaagcga catatacagc tttgggataa 2040

ttctacttga gctgataact ggtcaacctg ccataagaag agacttcaat ggagaaatta 2100

ttcgaataca agagtgggtt acaccaatat ttgagaatgg cgatgtcaga agcatagttg 2160

atcccaggct acagggagac ttcgatacca attctgcctg gaaagctgtg gaaatagctc 2220

tgtcctgtgt actgaataca gcaactcgaa ggccagacat gactgatgta ttgattgaac 2280

tgaaggaatg tttgggtatg gtaacagctg ttgttggatc tcagaggatg gatagaggaa 2340

ggacaagatc catcaattca cttgaaatga ggtctttgga aatctacact gaaacggctc 2400

ctagtccaag gtag 2414

<210> 4

<211> 2484

<212> DNA/RNA

<213> Manihot esculenta Crantz

<400> 4

atggatggac ggaaagctgt ggtttcagtc acactatggt taatctttgt tctgctgctg 60

tctgctactt tcagaaacat aaaactagct gcagcaaagg atgacaagct tgctgaaaaa 120

agaaagcttg ccgccagtaa ctcgagtttc ctgagcattg attgtggggt ggatcaagat 180

tatttcgatg agaaaatggg aatattttac aagtctgaca aagatttcat aagcaccgga 240

gaaaacaagt acagtttaag gttctttcca aaaggaagaa agaattgcta caaattgaga 300

cctgagcatg gcaagaatca aaattatctt atcagagcat tcttcaaata tgggaattac 360

gatgccctga ataaaattcc aaaatttgat gtgtatctcg gggctaattt ttggatgaca 420

gtccaaccaa taaatgtaac agcaacattg ctggaagaaa ttatttattt ttcattaacc 480

gatgttatta acatatgtct cgtaaatact ttatctggaa aaccttttat ctcagcacta 540

gaactcagac tgctgaataa ttctatttat aaaatcgagt ccaaatccct atatgtactt 600

ggaagatatg accttggcaa ctcaaccaac aggatggtca ggtacaagaa tgatgtgtat 660

gatcgcttgt gggatactct tacccctgct gactgcattg cattgaacac caccgcaaat 720

attgaggttc agagcatcaa caacaccctt aaaacgccgg ttgaagtttt aagaactgcc 780

gcccaagtac gaagtcccct cagatcattg ggtaactcct ttcccggtgt gtatattgct 840

gccgaaagtg aatactatgc ctgctttcac tttgccgaaa tattaccgat ttcacaaggc 900

aaagggaaac accgacggga attcaccatc aattttaacg gtgccaacta tggacatatt 960

accctccaat acttgaaccc acttaccatg tgttatggac ctcgtaaatc aagagatagt 1020

ggctttgtgg agttctccat caatcaaact ggacgctctg accttccacc tatcctcaac 1080

gcctttgagc ttttttacgt aatgccgcca cttggttcgc ccacagaccc agcagatgtt 1140

gatgctatga tggccatccc acaaatctac accattgata aagatgataa ctggcaaaga 1200

gacccgtgtc tgccaaggga tattacttgg actggcttga actgcagcta tgacagtaat 1260

tctccaagga tcatctctct ggacctctgt gcaagtaggc tgactggaaa aatatcttcc 1320

tcattcttca atctcacagc catacgatac ttagatttat caggcaatga actgactgga 1380

acagttccag aatttttagc acaactgcca aatttaactg ttctaaattt aagtggaaac 1440

aagctcacag gtacagttcc tcagtctctt gtccaaaaag ctaacaatag actgctgcag 1500

ctgagcttgg aaggcaatcc aagtctatgc cagacagatt cctgccagaa aaagaagcgc 1560

agtgttcttc ttccaattgt tacatccttt gcaacagttc tggtgcttct cttcctgagc 1620

actatatttt tcttttggag aatgaaaaga caagcaacat cacaatctaa gaaagaagga 1680

ctagtaattt caacgaatcg gtcctttagt tattcggaga tcgtaagtat tactaataac 1740

tttgaaacca tcatcggaga aggagggttc ggaaaagttt actttgggac tctgaaagat 1800

aacgttcaag ttgctgtcaa attgctttct caaaattcaa ggcaaggcta caaggaattt 1860

caatctgagg cacaactgtt gatgattgtt catcacagaa acttggtttc tctaattggc 1920

cactgtgatg accgtcataa taaggctctg atttatgaat acatggctaa tggaaatttg 1980

cgagagcatt taacagagac aagtgggagt actttgaatt ggaatgaaag gcttcatatt 2040

gcagcagatg cagcacaggg actggaatat ctacataacg gttgcaagcc accgataatc 2100

cacagggatt tgaagacttc caacatctta ttgaatgaaa aattgcgagc aaagatatct 2160

gatttcgggc tatcaagagc tttcgcaaat gaaagtggtt cacatataac aactcgccct 2220

gcaggcacac tcggctacct ggatcctcag gctcagtcat caggaaactt taataagaaa 2280

agcgacatat acagctttgg gataattcta cttgagctga taactggtca gcctgccata 2340

agaagagacg tcaatggaga aattattcga atacaagagt gggttacacc aataattgag 2400

aatggcgatg tcagaagcat actgttgttg gatctcagag gatggataga ggaaggacaa 2460

gatccatcaa ttcacttgaa atga 2484

<210> 5

<211> 2643

<212> DNA/RNA

<213> Manihot esculenta Crantz

<400> 5

atggagatca aatttcttgt ccttggtttt ttttcacttg caattctgca ggctcagggc 60

caggatcaat cagactttat cagccttgac tgtgggttgc cagcaaatac tagctattct 120

gatgaaaaaa ccagcctata ttacatttca gatgcgtact tcatttacag cggcgtagct 180

aggagcttgc cacctgaatt cagtacaaat agcattgaca ggcagctttg gtacgtcagg 240

agctttcctc agggcatcag aaattgctac aacgtaacgg ttactgcaaa taccaaatat 300

ttgatcagag ccacttttat gtatggaaat tacgataatt taaatcagct gccggaattt 360

gacctgcaca taggacctaa caaatgggtt acagtgcaaa tacaaaatac atccactcct 420

gtaatagagg agatcatata tgatgcttca gtgagttaca tatgtatatg tcttgtaaat 480

ataaactctg aaactccatt tatttcagca ttggaattaa ggcctttgag aaatactacg 540

tatgtaactc agtctggagc tggagcgctt gccttaatcg gacaacggct ggaccttggt 600

tcaataagta atcaagatca tgtcaggtat ccttatgata tctttgatcg tttctggtat 660

ccctttcatt taatccaact gacagacata agtaccaatc tcactattga tgtcggaagc 720

cataacgatt accagccgcc gccaatcgtg atgaggagcg cagttatacc agcaaacagc 780

agcaatccct tgcaactatc cataggcgat agcgtgaaat acaaaaattt ccaattttat 840

atgtacatgc acttcgccga tgttgtgaag cttgaagcta accagtcgcg taaattcaac 900

atttctcgaa atggaaccat ctggttcgga cctattattc ctctttactt gtatacaagc 960

actgcataca gccaatcagc catcactgga ggagactatc tcttttccct cttcaaagtt 1020

gatgggtcaa ctctgccgcc tcttcttaat gcagttgaag tttattactt gattgagctc 1080

ttacaatcag aaacaagcag acctgatatt gacgcaatca tgagcatcaa gtcaacgtat 1140

ggaataagca gaaactggca aggagatcca tgtgctcctc aggagtatgt gtgggatggt 1200

cttaactgca gctataatgg gacagcacct ataatcacat cactgaactt atcctccagc 1260

ggattgacag gtgagatagc ttctgatata gccaatctga aatcgctaag atctctggac 1320

ttatcaaaca atagtttaat tggacctgtg cctgattttc tctctaagtt ggtgtccttg 1380

gaagtactaa acctgacagg caacaggctc acaggtagaa ttccagttga cctcttaaag 1440

agaaggcaaa atggcttgct attgctaagt gttgacggaa atccagaact ttgtgcattg 1500

gattcatgca atgataataa caagaagaat tacgttgttc cagtaacagt atcggtttct 1560

gcattatttg cgatagtagc agcaatatca ctggcaatat gccttaaaag aagaaagaaa 1620

caagcagcag tagctggtga gatgatggaa gctcaagcga ggatattaga gtcaagaaaa 1680

cgacaattca cctactctaa agtcctggaa atgaccaata actttgaaag agttcttggc 1740

aggggaggat ttggaaccgt ttaccatggc ctcttgggtg atactgaagt agctgtaaag 1800

acgctttctc catcctcagt tcaaggctac aaagagttcc aggccgaggt taaacttctt 1860

ttgagagttc atcacaaaaa cttgactacc ctcgtcggat actgcgagga acccagccat 1920

atggtgctca tctatgaata catggccaat ggagacttaa aacaccatct ctcaggcgat 1980

gacccagtaa atatcttaag ctgggaggag agacttcgaa tagccattgg cgcagcacaa 2040

ggattggagt atcttcacaa tggctgcaag ccgccaattg tgcacagaga cgtgaagact 2100

acaaacatct tgttggacag taatttccaa gccaaattag ctgattttgg gctgtccaga 2160

atttttccaa ccgaagatgg ctctcatgtg tcgacaactg ttgctggcac tccaggctac 2220

ctggatcccg agtaccatgt aacaaactgg ttgactgaaa aaagcgatgt ttatagttat 2280

ggagttgttc ttctagagat cattacaagc cgaccagtga ttgccaaaac tggtgacagg 2340

actcatctga gcacttgggt tagttccatg cttgacaaag gagatattaa gaccatttct 2400

gatccaagat tgcttggaga tttcgacact aattctgtct ggaaagttac agaacttgca 2460

atggcttgcg tttctgaaat ttctgctgaa agaccaacta tgagccaagt gttggtggaa 2520

ctgaacgagt gtttagcaac agagacggct cgaacaagag agggacgtag tcaatcaaat 2580

agtaattcta ttgaatttac gaacgtggat ctgtcttctg gactatttcc ccagccaagg 2640

tag 2643

<210> 6

<211> 2550

<212> DNA/RNA

<213> Manihot esculenta Crantz

<400> 6

atggaaatat tttacaagtc tgacaaagat ttcacaagca ccggagaaaa caagtacgtg 60

tctcccggat acgatgatga tgatgattct tcttatactg gacgtatatt gcatagttta 120

aggttctttc caaaaggaag aaagaattgc tacaaattga gacctgagaa tggcaagaat 180

caaaattatc tcatcagagc attcttcaaa tatgggaatt acgatgccca gtaccaaatt 240

ccaaagtttg atgtgtatct cgaggctaat ttttggatga ctgtctatct tccaaatgca 300

tcatacccgt tgatgtatga gattatttat ttttcattaa ccgatattat taacatatgt 360

ctcgtaaata ctttatctgg aacacctttt atctcagcac tagaactcag actgctgaat 420

aattctgttt ataaaatcga gtccaaatcc ctaaatacac ttagaagaga tgaccttggc 480

aactcaacca acaggatggt caggtacaag aatgacgtgt atgatcgcat atggtataca 540

agtgctgcta ctgactacat ttcattgaac accacctcaa atattgaggt tcagagcatc 600

aacaacaccc ttaaaacgcc ggttgacgtt ttaagaactg cgctccaatt acgaagtccc 660

ctcagatcat tgggtcgctc ctttcccggt ttgaataatt acggtaatga aaatgaatac 720

tatgcctgct ttcacttcgc cgaaatatta ccgatttcac aaggcaaagg caaacgccca 780

agggaattca ccatcaattt taacggtgcc aactatggac gtactaccct ccaatatttg 840

aacccactta ccacttgtta cggacctcgt aaatcaagag ttaatggcct tgtggatttc 900

tttatcaatc aaactgtacg gtctgacctt ccacctatcc ttaatgcctt tgagctcttt 960

tatgtaatgc cgccacttgt ttcgcccaca gacccagcag atgttgatgc tatgacggtc 1020

atccagcaaa tgtacaatat taataaagat gatagctggc aaggagaccc ctgtctgcca 1080

agagattatt cctgggctgg cttgaactgc agctatgaca ctaattcccc aagaatcatc 1140

tctctggacc tcagtggaag taagttgact ggaaaaatat cttcctcatt ctccaatctc 1200

acagccatac gattcttaga tttatcaggt aatgaactga ctggaacagt accagaattt 1260

ttagcacaac tgccaaattt aactgttcta aatttaagtg gaaacaagct aacaggttca 1320

gttcctcagt ctcttgtcca aaaagctaac aatagactgc tgcagctgag cttggaaggc 1380

aatccaagtc tttgccagac agattcctgc gagaaaaaga agcacaataa tgttctcctt 1440

ccagttgtta tatcctttgc aacagttatg gtgcttctct tcctcagcag tatatttttc 1500

ttttggagaa tgaaaagaca agaagcaaca tcacaatcta agaaggaagg actagtaatt 1560

tcaacgaatc ggtcctttag ttattcggag atcgtaagta ttactaataa ctttgaaacc 1620

atcatcggag aaggagggtt cggaaaagtt tactttggga ctctgaaaga taacgttcaa 1680

gttgctgtca aattgctctc tcaaaattca aggcaaggct acaaggaatt tcaatctgag 1740

gcacaactgt tgatgattgt tcatcacaga aacttggttt ctctaattgg ctactgtgat 1800

gaccgtcata ataaggctct gatttatgaa tacatggtta atggaaattt gcgagagcat 1860

ttatcagaga caagtggaag tactttgaat tggaatgaaa gacttcatat tgcagcagat 1920

gcagcacacg gactggaata tctacataac ggttgcaagc caccgataat ccacagggat 1980

ttgaagactt ccaacatctt attgaatgaa aaattgcgag caaagatatc agatttcggg 2040

ctatcaagag ctttcgcaaa tgaaagtggt tcacatataa caactcgtcc tgcaggcaca 2100

atcggctacc tggatcctca ggctcagtca tcaggaaact ttaataagaa aagcgacata 2160

tacagctttg ggataattct acttgagctg ataactggtc aacctgccat aagaagagac 2220

gtcaatggag aaattattcg aatacaagag tgggttacac caataattga gaatggcgat 2280

gtcagaagca tagttgatcc caggctacag ggagacttag ataccaattc tgcctggaaa 2340

gctgtggaaa tagctctgtc ctgtgtactg aatacagcaa ctcgaaggcc agacatgact 2400

gatgtattga ttgaactgaa ggaatgtttg ggtatggtaa cagctgttgt tggatctcag 2460

aggatagata gaggaaggac aagatccatc aattcacttg aaatgaggtc tttggaaacc 2520

tacactgaaa cggctcccag tccaaggtag 2550

<210> 7

<211> 2577

<212> DNA/RNA

<213> Manihot esculenta Crantz

<400> 7

atggttgctt cgaggaagct cgaggatgat ggcactaatc atgtgaagtt acaatacttc 60

ctctttctat tgcttcgcac ctttgctttt acagctgtgg tcaaagctca agatcaatct 120

gggttcatca gcatagattg tggagtacca gataatgctt cttacactga taaaacaaca 180

tacttaaatt acgtttctga tgctgcattc attgacactg gcatcagtat gaagatatca 240

ccggaattca ggacatacga tatagaccag cagctacaaa atctcagaag ctttcctgag 300

ggaaacagaa actgctacaa tgttggattg aaaaagggca gtaaatattt gatcagagct 360

actttcatgt atggaaatta tgatggtcaa aacaaagcac cacagtttga tattcatcta 420

ggatctacca aatgggatac tgttgaaaca attaatgcaa ccatgattat tacgaaggag 480

atcatacatg tgaccaccat gagctatcta cttgcctgcc tagcaaacac tggctctggg 540

acacctttca tatcagcttt agaattcagg cctctcaaga atgcatcata caactatact 600

gaatcaggat cattagtacg cttcactcga ttagatattg gttcaacaac aaatcaaaca 660

gtcaggtacc cagaggatgt ttacgaccgc atctgggcgc cttatcattc ctttaagtgg 720

gcagagataa atacctcaca aagcattgat tcacaagctc agaataatta ccggccacca 780

tcagttgtca tgaggactgc tggcacacct gcaaatgcta gcgaggcaat gacactccat 840

ataaacatgg aagataacac tctcaaattt ctggtctaca tgcacttctc tgaaattgta 900

agactccaag ccaaccagtc cagagaattc aacatctctt tcaacggaaa acactggttt 960

ggacctgtag tccctaatta cttgtacaca acaactgtat atagtcgatc agcattgact 1020

gcaggacaat atcagtttac tctctataaa actgaatgct caacccttcc gccccttctc 1080

aatgcaattg agatttatta tgtgcttcca atctcacagt cccaaacaaa ccaagaagat 1140

gggtacttgt gggatggtct caattgcagc aacagtgata atgatatgct taggatcata 1200

tccttgaact tatcctcaag tggattgact ggcgaaataa ctcgttccat atccagtctc 1260

aaattgttag aatctctaga cctatcaaat aatagtttga caggatcagt gcctgatttt 1320

ctttctcaga tgtcatcctt gaaattcctg aacttgacag gaaataaact cagtggctca 1380

gttccaactt cactcattga gaggtcaaag cagaatttgc tagtgctaag caaaaaggac 1440

aagaactggg ttgttccagt agttgcctcg attgctggag gggtcattgc cataggtgca 1500

ttagctatca tattctgcag ccttaaaaga agaaaacaaa aagttgacct tgctaaagta 1560

ttactagcta aagaagagga agccaaaacc aacaacgcat atgagtcact gaaagcaaaa 1620

ggacgacact tcacatactc tgagatcctg agtattacca acaattttga aagggttctt 1680

ggtaagggcg gatttggaac agtttaccat ggctacttag atgatgacac tcaagtagct 1740

gtgaagatgc tctctccatc atcagtccag gggtataggc agtttcaggc agaggtcaac 1800

cttcttttgc gagttcatca ccgaaacttg acaacacttg ttgggttctg tgacgaaggc 1860

accaacaggg gactcatcta tgagtacatg gttaatggag atttagaaaa atttttgtca 1920

ggttcaaaca aatatatatt gacatgggag aggagactaa acatagtagt tgaagcagca 1980

aaaggatggg aatatctgca caatggaagc aaaccaccaa ttgtgcacag ggatgtgaag 2040

acatcaaaca ttttattaaa tgacaatttc caagcaagac ttgctgattt tggactgtcc 2100

agatctttcc cggttgaagg tggcactcat gtaacaacag ttgttgctgg cacccctggc 2160

taccttgatc ctgagtattc tgtgacaaac tggctaacag agaaaagtga tgtgtatagc 2220

ttcggggttg ttctcttaaa gataatcaca ggaaggccag tgattgcagg aggaggtgag 2280

aggaatattc atctgcgtca atgggttagt tctctgctcg caaacgggga cattaaaaat 2340

gttgttgatc caaggttagg tggagatttc gacaataatt ctgtctggaa agcagtagaa 2400

atagcaatgg cctgtacaac cccaatttct gctggcaggc caactatgca tcaagtagtg 2460

acagaactaa atgaatgctt agcaacagaa agaactgaaa agaggaaggg acattctagg 2520

agtgaaccaa tagcttccat tgaaatgatt actgaatcaa ctcccctagc aagatag 2577

<210> 8

<211> 2766

<212> DNA/RNA

<213> Manihot esculenta Crantz

<400> 8

atgaatatgg atagacggaa agcactggtt tcagttgcgc catggctaat cttcgctctg 60

ctctatacta cttatggaaa cttaaaacta gctgcagcaa gtaatgatga tgtgctcgct 120

gaaagaatga agcttgccgc aaaaagctcg ggctccataa gcatcgattg tggagcagat 180

gaagattacg ttgatgaaaa tgttggattg ctttacaagt cagatagaga tttcataagc 240

actggaaaaa attacgttgc acaaccagat gccacccgcc tacaggcttt tacagaacgg 300

ctgtgtaaaa atttaagaag tttcccaacg gggaaaaaga actgctacaa tcttcgacct 360

gaacaaggaa aaaaccataa ttactacatc agagctctct tttgttatgg aaattacgat 420

ggcaagaatc aaccaccaga gtttgatgtg tatgtagggg ttaactattg ggtgacagtg 480

aatggattag ctggttgggc ttggacaaca cgggagatct ttcatttttc ttcaactgat 540

gtcattcatg tctgccttgt aaacaaggac tttggaatac cttttatttc agcattggag 600

ttgcatcctg tgaatgattc catttataaa agtgaatttg gatcattatc tgttgctaga 660

agatctcaac tttcctatcc taccgccatg aatttcaggt acaaggatga tgtctttcac 720

cgcatctgga catcttacgg gcttgcaaac tccacttggg ttaatgccag tgccacatca 780

gatatgcaag tacatcaaac tgatgatccc tataagccac ccgttgaaat ttttagaact 840

gcagcccagc cacaaaaagg gcttcattca ttgagataca acattagctt tatttgctct 900

ccatcatgtg atttctatgt ctactttcac tttgctgaaa ttgtgaagat tcctcaagat 960

caacgaagag aattcacaat tactcttaat ggtctcaact atggacccgt cagcttggaa 1020

tacttgaagc cacagactat acctcttaaa tcaccagctc caggagatat caactttact 1080

ataaatccaa caagcaactc tgaccttcca cccattctca gtgcctttga gattctcagt 1140

gtacttaagc ttccactttc tccaacagac caatcagatg ttgatgccat catggacatc 1200

atgcaattat acaatataaa tggagatgaa ttgcaaggag acccatgtgt gccaagagaa 1260

ctttcatgga atggtttgaa ctgtagttat gacagtaatc ctccaaggat aatctctctg 1320

gaccttagtt ccaggaaatt gactggggaa atagctccga cactctccgt cctcaaggca 1380

attcagtcat tggatttatc atacaataag ttgactggaa gtctgcccaa ttttttagta 1440

gaactgcaaa atttaactcg gctggattta tcgtacaatg atttgacagg accgctgcca 1500

gaatttttag cacaactgcc gaacttaaat actttaaatt taattgggaa caagctctct 1560

ggatccattc cacagtccct tatagaaaag tctaacaatg gaatgttgca gctaagcatc 1620

gacaaaaacc caaatttgtg cccatcgggt tcgggttcct gtgagaaaaa gaagcatttt 1680

cttcttccag ttgttgcatc aattatatca accttgattc ttcttctact gatagctatc 1740

tcttggggat acagaagaaa gaaagaaaca caaaattcaa atacatgggc ctttagttac 1800

tcagagattg ttagcattac ggaggacttt aaaaccatca ttggaggagg gggctttgga 1860

aaagttttct ttggaactct aaaagatggc actcaagttg caattaagct gttatctcaa 1920

tcatcaaggc aaggctacaa agaatttcaa gcagaggtgc agcttttgat gattgttcat 1980

cacaggaact tagtttcact aattgggtac tgtagagata gtcataacat ggcattggtg 2040

tatgagttca tggttaatgg gaacttgcga gagcatttat cagagacaag tggaagtatt 2100

ctgacttgga aagagagaat tcaaattgca gtagatgcag cttatgggct ggagtatcta 2160

cataatggct gcaaaccacc aattatccac agggatctga aggcttccaa catcttattg 2220

aatgaaaaat tgcaagcaaa gatcgctgat tttggattat ctagggcttt cacaagcgaa 2280

agtggtacac atgtatcaac ttgtccagct ggaacattcg gctacgtgga tcctgaggct 2340

caggcatcag gaaacttcaa caaaaaaagt gatgtttata gctttggggt tatactactt 2400

gagctaataa ctggtcagcc tgcactaata agaaacaccg ctggaaactt catttgcata 2460

catcagtgga ttagacctat aattgacaca ggtgatattc ggaccatagt tgatgccaga 2520

ctgcaaggac aattcaatgt caattctgct tgggaagtta tggagattgc cttttcctgt 2580

gtatcagatg ttgcaataca gaggccagac atgagtcatg tactggctgg attacaggaa 2640

tgcttgacga cattaatggc tgttgaagca tcccaaaaat tggaagctat gaccacaaga 2700

tcaagccatt cacttcgaat gaggcatcta gatattgata ctgatatggc tcctagtgca 2760

aggtaa 2766

<210> 9

<211> 915

<212> PRT

<213> Manihot esculenta Crantz

<400> 9

Met Val Glu Lys Gly Arg Gly Ile Trp Ala Trp Gly Ser Ile Ile Cys

1 5 10 15

Leu Ile Leu Leu Gln Leu Leu Ala Asn Cys Phe Gln Thr Lys Gly Ser

20 25 30

Asn Val Phe Ala Trp Arg Phe Thr Arg Tyr Gly Asn Tyr Glu Thr His

35 40 45

Tyr Thr Gln Lys Arg Ser Arg Arg Lys Leu Asp Asp Val Ala Gly Ser

50 55 60

Ile Asn Met Asp Cys Gly Ile Pro Glu Asp Phe Ser Tyr Thr Asp Ala

65 70 75 80

Ser Thr Gly Ile Pro Tyr Ile Ser Asp Gly Gly Phe Ile Asp Thr Gly

85 90 95

Val Asn Lys Asn Ile Ser Ala Asn Phe Ser Ser Asp Asn Pro Pro Thr

100 105 110

Ser Leu Met Thr Val Arg Ser Phe Pro Gln Gly Asn Arg Asn Cys Tyr

115 120 125

Thr Leu Lys Pro Pro Glu Gly Lys Ala Ser Ile Tyr Leu Ile Arg Ala

130 135 140

Ser Phe Met Tyr Gly Asn Tyr Asp Asn Leu Gly Lys Leu Pro Glu Phe

145 150 155 160

Ser Leu Tyr Leu Gly Val Asn Leu Trp Asp Lys Val Lys Phe Asp Asn

165 170 175

Ser Ser His Val Val Asn Lys Glu Ile Ile His Val Pro Thr Met Asp

180 185 190

Asp Val Tyr Val Cys Leu Leu Asn Thr Gly Ser Gly Thr Pro Phe Ile

195 200 205

Ser Ala Leu Glu Leu Arg His Phe His Asn Ser Thr Tyr Lys Ala Glu

210 215 220

Ser Ala Ser Leu Val Leu Tyr Gln Arg Leu Asp Phe Gly Ser Ile Thr

225 230 235 240

Asn Glu Ile Val Arg Tyr His Asp Asp Val Tyr Asp Arg Val Trp Phe

245 250 255

Pro Tyr Asn Cys Pro Gln Ser Ala Ala Ile His Thr Pro Ser Ala Val

260 265 270

Asp Ser Leu Val Ser Thr Asp Phe Asn Leu Pro Ser Lys Val Met Gln

275 280 285

Thr Ala Val Gln Pro Ile Asn Ala Asp Lys Pro Leu Asn Phe Gly Phe

290 295 300

Asp Ile Gly Asp Pro Asn Glu Lys Leu Phe Ile Tyr Met His Phe Ala

305 310 315 320

Glu Ile Glu Thr Val Gln Lys Asn Gln Ser Arg Glu Phe Asn Ile Glu

325 330 335

Leu Asn Gly Val Leu Leu Glu Glu Gly Val Val Leu Lys Tyr Leu His

340 345 350

Ser Thr Thr Ile Pro Ile Glu Ser Val Arg Gly Ala Lys Leu Ser Phe

355 360 365

Ser Leu His Lys Leu Pro Asn Ser Thr Leu Pro Pro Ile Leu Asn Ala

370 375 380

Leu Glu Ile Tyr Leu Val Lys Asp Phe Trp Gln Gln Pro Thr Asp Lys

385 390 395 400

Glu Asp Val Asn Ala Ile Glu Asp Ile Lys Ser Ile Tyr Asn Val Asn

405 410 415

Lys Val Trp Arg Gly Asp Pro Cys Ile Pro Leu Pro Ser Trp Asp Gly

420 425 430

Leu Tyr Cys Asn Asp Asn Gly Tyr Asp Pro Pro Arg Ile Ile Ser Leu

435 440 445

Asn Leu Ser Ser Ser Gly Leu Thr Gly Glu Ile Asp Arg Thr Leu Ser

450 455 460

Asn Leu Lys Leu Leu Gln His Leu Asp Leu Ser Glu Asn Ser Leu Thr

465 470 475 480

Gly Arg Val Pro Glu Phe Leu Ser Glu Leu Pro Asn Leu Arg Thr Leu

485 490 495

Asn Leu Ala Gly Asn Lys Leu Ser Gly Ser Val Pro Phe Ala Leu Val

500 505 510

Glu Arg Ser Asn Asn Gly Leu Leu Ser Leu Arg Leu Asp Gly Asn Pro

515 520 525

Glu Leu Cys Leu Leu Ser Pro Cys Lys Glu Glu Arg Lys Arg Ala Trp

530 535 540

Val Pro Ile Val Ala Thr Ile Val Pro Leu Val Val Ile Leu Met Ala

545 550 555 560

Met Ala Leu Val Ile Leu Cys Ile Tyr Lys Arg Lys Asn Val Ala Arg

565 570 575

Lys Phe Ile Asp Arg His Lys Glu Glu Gly Ser Ser Leu Lys Ser Asp

580 585 590

Asn Arg Gln Phe Thr Tyr Ala Glu Ile Val Arg Ile Thr Asn Asn Phe

595 600 605

Ser Thr Val Ile Gly Lys Gly Gly Phe Gly Thr Val Tyr His Gly Tyr

610 615 620

Leu Ser Asp Asp Thr Gln Val Ala Val Lys Met Leu Ser Pro Thr Ser

625 630 635 640

Asp His Gly Ser Ala Gln Phe Arg Thr Glu Ala His Leu Leu Met Arg

645 650 655

Val His His Arg Asn Leu Ala Ser Phe Ile Gly Tyr Cys Asn Glu Ser

660 665 670

Arg Asn Thr Gly Ile Val Tyr Glu Tyr Met Ala Cys Gly Asn Leu Glu

675 680 685

Asn Tyr Leu Leu Asp Lys Ala Val Gln Val Leu Ser Trp Lys Glu Arg

690 695 700

Val Gln Ile Ala Leu Glu Ala Ala Gln Gly Leu Glu Tyr Leu His Asn

705 710 715 720

Gly Cys Lys Pro Pro Ile Val His Arg Asp Val Lys Ser Ala Asn Ile

725 730 735

Leu Leu Asn Asp Asn Leu Gln Ala Lys Ile Ala Asp Phe Gly Phe Ser

740 745 750

Lys Ile Phe Pro Ser Asp Ser Lys Ser His Leu Ser Thr Gly Val Val

755 760 765

Gly Thr Val Gly Tyr Leu Asp Pro Glu Tyr Tyr Ser Ser Gly Arg Leu

770 775 780

Thr Glu Arg Ser Asp Val Tyr Ser Phe Gly Ile Val Leu Leu Glu Leu

785 790 795 800

Ile Thr Gly Gln Ser Ala Val Ile Lys Asn His Asp Gln Asn Ile His

805 810 815

Ile Val Asn Trp Val Ser Pro Tyr Ile Glu Arg Gly Asp Ile Arg Asn

820 825 830

Val Val Asp Pro Arg Leu Asp Gly Asn Phe Asp Thr Asn Ser Val Trp

835 840 845

Lys Phe Val Glu Ile Ala Met Ser Cys Val Pro Ser Ile Ser Ile Gln

850 855 860

Arg Pro Thr Met Asn His Val Val Ala Glu Leu Lys Glu Cys Leu Val

865 870 875 880

Thr Glu Ile Gly Arg Glu Gln Ser Cys Lys Val Glu Gly Gln Ile Arg

885 890 895

Asn Ser Phe Glu Met Val Thr Val Asp Leu Glu Thr Glu Met Gly Pro

900 905 910

Glu Ala Arg

915

<210> 10

<211> 850

<212> PRT

<213> Manihot esculenta Crantz

<400> 10

Met Glu Ile Phe Tyr Lys Ser Asp Lys Asp Phe Thr Ser Thr Gly Glu

1 5 10 15

Asn Lys Tyr Val Ser Pro Gly Tyr Asp Asp Asp Asp Asp Ser Ser Tyr

20 25 30

Thr Gly Arg Ile Leu His Ser Leu Arg Phe Phe Pro Lys Gly Arg Lys

35 40 45

Asn Cys Tyr Lys Leu Arg Pro Glu Asn Gly Lys Asn Gln Asn Tyr Leu

50 55 60

Ile Arg Ala Phe Phe Lys Tyr Gly Asn Tyr Asp Thr Gln Asn Arg Met

65 70 75 80

Pro Lys Phe Asp Val Tyr Leu Gly Val Asn Phe Trp Met Thr Val Tyr

85 90 95

Pro Ser Asn Ala Ser Gln Pro Trp Met Asn Glu Ile Ile Tyr Phe Ser

100 105 110

Leu Thr Asp Val Ile Asn Ile Cys Leu Val Asn Thr Leu Ser Gly Thr

115 120 125

Pro Phe Ile Ser Ala Leu Glu Leu Arg Pro Leu Asn Asn Ser Ile Tyr

130 135 140

Lys Ile Glu Ser Lys Ser Leu Ser Thr Leu Arg Arg Asp Asp Leu Gly

145 150 155 160

Asn Cys Cys Arg Thr Val Arg Tyr Lys Asn Asp Val Tyr Asp Arg Ile

165 170 175

Trp Asp Thr Arg Asp Thr Ser Ala Ile Asn Glu Cys Ile Ser Leu Asn

180 185 190

Thr Thr Ser Asn Ile Glu Val Gln Ser Ile Asn Asn Thr Leu Lys Thr

195 200 205

Pro Val Asp Val Leu Arg Thr Ala Val Gln Leu Arg Ser Pro Leu Arg

210 215 220

Ser Phe Gly Arg Tyr Tyr Ser Gly Gly Tyr Asn Tyr Gly Asn Glu Ser

225 230 235 240

Glu Tyr Tyr Ala Cys Phe His Phe Ala Glu Ile Leu Pro Ile Ser Gln

245 250 255

Gly Lys Gly Lys Arg Pro Arg Glu Phe Thr Ile Ser Phe Asn Gly Ala

260 265 270

Asn Tyr Arg Arg Ile Thr Leu His Tyr Leu Asn Pro Leu Thr Thr Cys

275 280 285

Tyr Gly Pro Arg Lys Ser Arg Val Asn Gly Phe Val Asp Phe Ile Ile

290 295 300

Asn Gln Thr Val Arg Ser Asp Leu Pro Pro Ile Leu Asn Ala Phe Glu

305 310 315 320

Leu Phe Tyr Val Met Pro Pro Leu Val Ser Pro Thr Asp Pro Ala Asp

325 330 335

Val Asp Ala Met Thr Val Ile Gln Gln Met Tyr Asn Ile Asn Lys Asp

340 345 350

Asp Ser Trp Gln Gly Asp Pro Cys Leu Pro Arg Asp Tyr Ser Trp Ala

355 360 365

Gly Leu Asn Cys Ser Tyr Asp Thr Asn Ser Pro Arg Ile Ile Ser Leu

370 375 380

Asp Leu Ser Gly Ser Arg Leu Thr Gly Lys Ile Ser Ser Ser Phe Ser

385 390 395 400

Asn Leu Thr Ala Ile Arg Phe Leu Asp Leu Ser Gly Asn Glu Leu Thr

405 410 415

Gly Thr Val Pro Glu Phe Leu Ala Gln Leu Pro Asn Leu Thr Val Leu

420 425 430

Asn Leu Ser Gly Asn Lys Leu Thr Gly Ser Val Pro Gln Ser Leu Val

435 440 445

Gln Lys Ala Asn Asn Gly Leu Leu Gln Leu Ser Leu Glu Gly Asn Pro

450 455 460

Ser Leu Cys Gln Thr Asp Ser Cys Glu Lys Lys Lys His Asn Val Leu

465 470 475 480

Leu Pro Val Val Ile Ser Phe Ala Thr Val Met Val Leu Leu Phe Leu

485 490 495

Ser Ser Ile Phe Phe Phe Trp Arg Met Lys Arg Gln Glu Ala Thr Ser

500 505 510

Gln Ser Lys Lys Glu Gly Leu Val Ile Ser Thr Asn Arg Ser Phe Ser

515 520 525

Tyr Ser Glu Ile Val Ser Asn Thr Asn Asn Phe Glu Thr Ile Ile Gly

530 535 540

Glu Gly Gly Phe Gly Lys Val Tyr Phe Gly Thr Leu Lys Asp Asn Val

545 550 555 560

Gln Val Ala Val Lys Leu Leu Ser Gln Asn Ser Arg Gln Gly Tyr Lys

565 570 575

Glu Phe Gln Ser Glu Ala Gln Leu Leu Met Ile Val His His Arg Asn

580 585 590

Leu Val Ser Leu Ile Gly His Cys Asp Asp Arg His Asn Lys Ala Leu

595 600 605

Ile Tyr Glu Tyr Met Ala Asn Gly Asn Leu Arg Glu His Leu Thr Glu

610 615 620

Thr Ser Gly Ser Ile Leu Asn Trp Asn Glu Arg Leu Gln Ile Ala Ala

625 630 635 640

Asp Ala Ala Gln Gly Leu Glu Tyr Leu His Asn Gly Cys Lys Pro Pro

645 650 655

Ile Ile His Arg Asp Leu Lys Thr Ser Asn Ile Leu Leu Asn Glu Lys

660 665 670

Leu Arg Ala Lys Ile Ser Asp Phe Gly Leu Ser Arg Ala Phe Thr Asn

675 680 685

Glu Ser Gly Ser His Ile Thr Thr Arg Pro Ala Gly Thr Ile Gly Tyr

690 695 700

Leu Asp Pro Gln Ala Gln Ser Ser Gly Asn Phe Asn Lys Lys Ser Asp

705 710 715 720

Ile Tyr Ser Phe Gly Ile Ile Leu Leu Glu Leu Ile Thr Gly Gln Pro

725 730 735

Ala Ile Arg Arg Asp Val Asn Gly Glu Ile Ile Arg Ile Gln Glu Trp

740 745 750

Val Thr Pro Ile Ile Glu Asn Gly Asp Val Arg Ser Ile Val Asp Pro

755 760 765

Arg Leu Gln Gly Asp Phe Asp Thr Asn Ser Ala Trp Lys Ala Val Glu

770 775 780

Ile Ala Leu Ser Cys Val Leu Asn Thr Val Thr Arg Arg Pro Asp Met

785 790 795 800

Thr Asp Val Leu Ile Glu Leu Lys Glu Cys Leu Gly Met Val Thr Ala

805 810 815

Val Val Gly Ser Gln Arg Met Asp Arg Gly Arg Thr Arg Ser Ile Asn

820 825 830

Ser Leu Glu Met Arg Ser Leu Glu Ile Tyr Thr Glu Thr Ala Pro Ser

835 840 845

Pro Arg

850

<210> 11

<211> 804

<212> PRT

<213> Manihot esculenta Crantz

<400> 11

Met Glu Asp Phe Ile Asn Thr Gly Lys Asn Gly Asn Val Ser His Glu

1 5 10 15

Tyr Asp Tyr Lys Asp Pro Tyr Phe Gly His Val Phe Gln Ser Leu Arg

20 25 30

Phe Phe Pro Lys Gly Arg Arg Asn Cys Tyr Thr Leu Arg Pro Glu Gln

35 40 45

Gly Lys Asn Gln Asn Tyr Leu Ile Arg Ala Phe Phe Arg Tyr Gly Asn

50 55 60

Tyr Asp Ser Asn Asn Glu Val Pro Arg Phe Glu Leu Tyr Leu Gly Val

65 70 75 80

Asn Leu Trp Thr Thr Val Gln Pro Ser Ser Pro Asn Ser Val Arg Glu

85 90 95

Ile Ile His Phe Ser Leu Thr Asp Thr Ile Lys Ile Cys Leu Val Asn

100 105 110

Ile Gly His Gly Thr Pro Phe Ile Ser Ala Leu Glu Leu Arg Pro Leu

115 120 125

Asn Pro Ser Ile Tyr Arg Ile Glu Ser Lys Asp Leu Tyr Leu Val Glu

130 135 140

Arg Tyr Asp Leu Gly Asn Asn Ser Thr Asn Arg Tyr Val Arg Tyr Lys

145 150 155 160

Asp Asp Val Tyr Asp Arg Phe Trp Leu Pro Trp Thr Phe Val Asp Cys

165 170 175

Ile Ser Leu Asn Thr Thr Ser Asn Ile Asp Val Gln Asn Ser Ser Asn

180 185 190

Pro Leu Arg Thr Pro Leu Gln Val Leu Arg Thr Ala Val Gln Pro Met

195 200 205

Lys Ile Leu Gln Ile Ser Gln Gly Pro Arg Glu Phe Thr Ile Asn Phe

210 215 220

Asn Gly Glu Asn Tyr Gly Pro Phe Thr Leu Gln Tyr Leu Gln Pro Leu

225 230 235 240

Ser Ile Cys Thr Arg Pro Arg Lys Ser Arg Thr Ile Gly Tyr Val Tyr

245 250 255

Phe Ser Ile Asp Pro Thr Val Gln Ser His His Pro Pro Ile Leu Asn

260 265 270

Ala Leu Glu Ile Phe Ile Pro Trp Met Asp Pro Val Ser Pro Thr Asp

275 280 285

Pro Ala Asp Val Asp Ala Ile Met Ala Ile Gln Gln Thr Tyr Asn Ile

290 295 300

Asn Lys Asp Asp Ser Trp Gln Gly Asp Pro Cys Met Pro Lys Ala Tyr

305 310 315 320

Ser Trp Thr Gly Leu Asn Cys Ser Tyr Tyr Asn Asn Ser Pro Arg Ile

325 330 335

Ile Phe Leu Asp Leu Ser Ala Ser Thr Leu Asn Gly Ser Ile Ser Ser

340 345 350

Ser Phe Ser Lys Leu Lys Ala Ile Gln Tyr Leu Asp Leu Ser Gly Asn

355 360 365

Glu Leu Thr Gly Thr Val Pro Glu Phe Leu Ala Gln Leu Pro Asn Leu

370 375 380

Thr Val Leu Asn Leu Thr Gly Asn Lys Leu Thr Gly Ser Val Pro Arg

385 390 395 400

Ser Leu Val Gln Lys Ser Asn Ser Gly Leu Leu Gln Leu Ser Leu Asp

405 410 415

Gly Asn Pro Asn Leu Cys His Thr Asp Ser Cys Glu Lys Gln Lys Arg

420 425 430

Asn Phe Pro Leu Pro Ile Val Val Ala Ser Val Ala Ile Leu Thr Val

435 440 445

Leu Leu Ser Ser Ile Phe Val Phe Trp Arg Met Lys Arg Arg Glu Gly

450 455 460

Thr Ser Gln Ser Lys Lys Glu Gly Leu Val Asn Ser Lys Asn Arg Ser

465 470 475 480

Phe Thr Tyr Thr Asp Ile Val Ser Ile Pro Asp Asn Phe Glu Thr Ile

485 490 495

Ile Gly Glu Gly Gly Phe Gly Lys Val Tyr Met Gly Thr Leu Lys Asp

500 505 510

Asn Thr Gln Val Ala Val Lys Leu Leu Ser Gln Asn Ser Arg Gln Gly

515 520 525

Tyr Lys Glu Phe Gln Ala Glu Ala Gln Leu Leu Met Ile Val His His

530 535 540

Arg Asn Leu Val Ser Leu Ile Gly Tyr Cys Asp Asp Arg His Asn Lys

545 550 555 560

Ala Leu Ile Tyr Glu Tyr Lys Val Asn Gly Asn Leu Arg Glu His Leu

565 570 575

Ser Glu Thr Ser Gly Ser Thr Leu Asn Trp Asn Glu Arg Leu His Ile

580 585 590

Ala Ala Asp Ala Ala His Gly Leu Glu Tyr Leu His Asn Gly Cys Lys

595 600 605

Pro Pro Ile Ile His Arg Asp Leu Lys Thr Ser Asn Ile Leu Leu Asn

610 615 620

Glu Lys Leu Gly Ala Lys Ile Ser Asp Phe Gly Leu Ser Arg Ala Phe

625 630 635 640

Thr Asn Glu Ser Gly Ser His Ile Thr Thr Arg Pro Ala Gly Thr Leu

645 650 655

Gly Tyr Leu Asp Pro Gln Ala Gln Ser Ser Gly Asn Phe Asn Lys Lys

660 665 670

Ser Asp Ile Tyr Ser Phe Gly Ile Ile Leu Leu Glu Leu Ile Thr Gly

675 680 685

Gln Pro Ala Ile Arg Arg Asp Phe Asn Gly Glu Ile Ile Arg Ile Gln

690 695 700

Glu Trp Val Thr Pro Ile Phe Glu Asn Gly Asp Val Arg Ser Ile Val

705 710 715 720

Asp Pro Arg Leu Gln Gly Asp Phe Asp Thr Asn Ser Ala Trp Lys Ala

725 730 735

Val Glu Ile Ala Leu Ser Cys Val Leu Asn Thr Ala Thr Arg Arg Pro

740 745 750

Asp Met Thr Asp Val Leu Ile Glu Leu Lys Glu Cys Leu Gly Met Val

755 760 765

Thr Ala Val Val Gly Ser Gln Arg Met Asp Arg Gly Arg Thr Arg Ser

770 775 780

Ile Asn Ser Leu Glu Met Arg Ser Leu Glu Ile Tyr Thr Glu Thr Ala

785 790 795 800

Pro Ser Pro Arg

<210> 12

<211> 827

<212> PRT

<213> Manihot esculenta Crantz

<400> 12

Met Asp Gly Arg Lys Ala Val Val Ser Val Thr Leu Trp Leu Ile Phe

1 5 10 15

Val Leu Leu Leu Ser Ala Thr Phe Arg Asn Ile Lys Leu Ala Ala Ala

20 25 30

Lys Asp Asp Lys Leu Ala Glu Lys Arg Lys Leu Ala Ala Ser Asn Ser

35 40 45

Ser Phe Leu Ser Ile Asp Cys Gly Val Asp Gln Asp Tyr Phe Asp Glu

50 55 60

Lys Met Gly Ile Phe Tyr Lys Ser Asp Lys Asp Phe Ile Ser Thr Gly

65 70 75 80

Glu Asn Lys Tyr Ser Leu Arg Phe Phe Pro Lys Gly Arg Lys Asn Cys

85 90 95

Tyr Lys Leu Arg Pro Glu His Gly Lys Asn Gln Asn Tyr Leu Ile Arg

100 105 110

Ala Phe Phe Lys Tyr Gly Asn Tyr Asp Ala Leu Asn Lys Ile Pro Lys

115 120 125

Phe Asp Val Tyr Leu Gly Ala Asn Phe Trp Met Thr Val Gln Pro Ile

130 135 140

Asn Val Thr Ala Thr Leu Leu Glu Glu Ile Ile Tyr Phe Ser Leu Thr

145 150 155 160

Asp Val Ile Asn Ile Cys Leu Val Asn Thr Leu Ser Gly Lys Pro Phe

165 170 175

Ile Ser Ala Leu Glu Leu Arg Leu Leu Asn Asn Ser Ile Tyr Lys Ile

180 185 190

Glu Ser Lys Ser Leu Tyr Val Leu Gly Arg Tyr Asp Leu Gly Asn Ser

195 200 205

Thr Asn Arg Met Val Arg Tyr Lys Asn Asp Val Tyr Asp Arg Leu Trp

210 215 220

Asp Thr Leu Thr Pro Ala Asp Cys Ile Ala Leu Asn Thr Thr Ala Asn

225 230 235 240

Ile Glu Val Gln Ser Ile Asn Asn Thr Leu Lys Thr Pro Val Glu Val

245 250 255

Leu Arg Thr Ala Ala Gln Val Arg Ser Pro Leu Arg Ser Leu Gly Asn

260 265 270

Ser Phe Pro Gly Val Tyr Ile Ala Ala Glu Ser Glu Tyr Tyr Ala Cys

275 280 285

Phe His Phe Ala Glu Ile Leu Pro Ile Ser Gln Gly Lys Gly Lys His

290 295 300

Arg Arg Glu Phe Thr Ile Asn Phe Asn Gly Ala Asn Tyr Gly His Ile

305 310 315 320

Thr Leu Gln Tyr Leu Asn Pro Leu Thr Met Cys Tyr Gly Pro Arg Lys

325 330 335

Ser Arg Asp Ser Gly Phe Val Glu Phe Ser Ile Asn Gln Thr Gly Arg

340 345 350

Ser Asp Leu Pro Pro Ile Leu Asn Ala Phe Glu Leu Phe Tyr Val Met

355 360 365

Pro Pro Leu Gly Ser Pro Thr Asp Pro Ala Asp Val Asp Ala Met Met

370 375 380

Ala Ile Pro Gln Ile Tyr Thr Ile Asp Lys Asp Asp Asn Trp Gln Arg

385 390 395 400

Asp Pro Cys Leu Pro Arg Asp Ile Thr Trp Thr Gly Leu Asn Cys Ser

405 410 415

Tyr Asp Ser Asn Ser Pro Arg Ile Ile Ser Leu Asp Leu Cys Ala Ser

420 425 430

Arg Leu Thr Gly Lys Ile Ser Ser Ser Phe Phe Asn Leu Thr Ala Ile

435 440 445

Arg Tyr Leu Asp Leu Ser Gly Asn Glu Leu Thr Gly Thr Val Pro Glu

450 455 460

Phe Leu Ala Gln Leu Pro Asn Leu Thr Val Leu Asn Leu Ser Gly Asn

465 470 475 480

Lys Leu Thr Gly Thr Val Pro Gln Ser Leu Val Gln Lys Ala Asn Asn

485 490 495

Arg Leu Leu Gln Leu Ser Leu Glu Gly Asn Pro Ser Leu Cys Gln Thr

500 505 510

Asp Ser Cys Gln Lys Lys Lys Arg Ser Val Leu Leu Pro Ile Val Thr

515 520 525

Ser Phe Ala Thr Val Leu Val Leu Leu Phe Leu Ser Thr Ile Phe Phe

530 535 540

Phe Trp Arg Met Lys Arg Gln Ala Thr Ser Gln Ser Lys Lys Glu Gly

545 550 555 560

Leu Val Ile Ser Thr Asn Arg Ser Phe Ser Tyr Ser Glu Ile Val Ser

565 570 575

Ile Thr Asn Asn Phe Glu Thr Ile Ile Gly Glu Gly Gly Phe Gly Lys

580 585 590

Val Tyr Phe Gly Thr Leu Lys Asp Asn Val Gln Val Ala Val Lys Leu

595 600 605

Leu Ser Gln Asn Ser Arg Gln Gly Tyr Lys Glu Phe Gln Ser Glu Ala

610 615 620

Gln Leu Leu Met Ile Val His His Arg Asn Leu Val Ser Leu Ile Gly

625 630 635 640

His Cys Asp Asp Arg His Asn Lys Ala Leu Ile Tyr Glu Tyr Met Ala

645 650 655

Asn Gly Asn Leu Arg Glu His Leu Thr Glu Thr Ser Gly Ser Thr Leu

660 665 670

Asn Trp Asn Glu Arg Leu His Ile Ala Ala Asp Ala Ala Gln Gly Leu

675 680 685

Glu Tyr Leu His Asn Gly Cys Lys Pro Pro Ile Ile His Arg Asp Leu

690 695 700

Lys Thr Ser Asn Ile Leu Leu Asn Glu Lys Leu Arg Ala Lys Ile Ser

705 710 715 720

Asp Phe Gly Leu Ser Arg Ala Phe Ala Asn Glu Ser Gly Ser His Ile

725 730 735

Thr Thr Arg Pro Ala Gly Thr Leu Gly Tyr Leu Asp Pro Gln Ala Gln

740 745 750

Ser Ser Gly Asn Phe Asn Lys Lys Ser Asp Ile Tyr Ser Phe Gly Ile

755 760 765

Ile Leu Leu Glu Leu Ile Thr Gly Gln Pro Ala Ile Arg Arg Asp Val

770 775 780

Asn Gly Glu Ile Ile Arg Ile Gln Glu Trp Val Thr Pro Ile Ile Glu

785 790 795 800

Asn Gly Asp Val Arg Ser Ile Leu Leu Leu Asp Leu Arg Gly Trp Ile

805 810 815

Glu Glu Gly Gln Asp Pro Ser Ile His Leu Lys

820 825

<210> 13

<211> 880

<212> PRT

<213> Manihot esculenta Crantz

<400> 13

Met Glu Ile Lys Phe Leu Val Leu Gly Phe Phe Ser Leu Ala Ile Leu

1 5 10 15

Gln Ala Gln Gly Gln Asp Gln Ser Asp Phe Ile Ser Leu Asp Cys Gly

20 25 30

Leu Pro Ala Asn Thr Ser Tyr Ser Asp Glu Lys Thr Ser Leu Tyr Tyr

35 40 45

Ile Ser Asp Ala Tyr Phe Ile Tyr Ser Gly Val Ala Arg Ser Leu Pro

50 55 60

Pro Glu Phe Ser Thr Asn Ser Ile Asp Arg Gln Leu Trp Tyr Val Arg

65 70 75 80

Ser Phe Pro Gln Gly Ile Arg Asn Cys Tyr Asn Val Thr Val Thr Ala

85 90 95

Asn Thr Lys Tyr Leu Ile Arg Ala Thr Phe Met Tyr Gly Asn Tyr Asp

100 105 110

Asn Leu Asn Gln Leu Pro Glu Phe Asp Leu His Ile Gly Pro Asn Lys

115 120 125

Trp Val Thr Val Gln Ile Gln Asn Thr Ser Thr Pro Val Ile Glu Glu

130 135 140

Ile Ile Tyr Asp Ala Ser Val Ser Tyr Ile Cys Ile Cys Leu Val Asn

145 150 155 160

Ile Asn Ser Glu Thr Pro Phe Ile Ser Ala Leu Glu Leu Arg Pro Leu

165 170 175

Arg Asn Thr Thr Tyr Val Thr Gln Ser Gly Ala Gly Ala Leu Ala Leu

180 185 190

Ile Gly Gln Arg Leu Asp Leu Gly Ser Ile Ser Asn Gln Asp His Val

195 200 205

Arg Tyr Pro Tyr Asp Ile Phe Asp Arg Phe Trp Tyr Pro Phe His Leu

210 215 220

Ile Gln Leu Thr Asp Ile Ser Thr Asn Leu Thr Ile Asp Val Gly Ser

225 230 235 240

His Asn Asp Tyr Gln Pro Pro Pro Ile Val Met Arg Ser Ala Val Ile

245 250 255

Pro Ala Asn Ser Ser Asn Pro Leu Gln Leu Ser Ile Gly Asp Ser Val

260 265 270

Lys Tyr Lys Asn Phe Gln Phe Tyr Met Tyr Met His Phe Ala Asp Val

275 280 285

Val Lys Leu Glu Ala Asn Gln Ser Arg Lys Phe Asn Ile Ser Arg Asn

290 295 300

Gly Thr Ile Trp Phe Gly Pro Ile Ile Pro Leu Tyr Leu Tyr Thr Ser

305 310 315 320

Thr Ala Tyr Ser Gln Ser Ala Ile Thr Gly Gly Asp Tyr Leu Phe Ser

325 330 335

Leu Phe Lys Val Asp Gly Ser Thr Leu Pro Pro Leu Leu Asn Ala Val

340 345 350

Glu Val Tyr Tyr Leu Ile Glu Leu Leu Gln Ser Glu Thr Ser Arg Pro

355 360 365

Asp Ile Asp Ala Ile Met Ser Ile Lys Ser Thr Tyr Gly Ile Ser Arg

370 375 380

Asn Trp Gln Gly Asp Pro Cys Ala Pro Gln Glu Tyr Val Trp Asp Gly

385 390 395 400

Leu Asn Cys Ser Tyr Asn Gly Thr Ala Pro Ile Ile Thr Ser Leu Asn

405 410 415

Leu Ser Ser Ser Gly Leu Thr Gly Glu Ile Ala Ser Asp Ile Ala Asn

420 425 430

Leu Lys Ser Leu Arg Ser Leu Asp Leu Ser Asn Asn Ser Leu Ile Gly

435 440 445

Pro Val Pro Asp Phe Leu Ser Lys Leu Val Ser Leu Glu Val Leu Asn

450 455 460

Leu Thr Gly Asn Arg Leu Thr Gly Arg Ile Pro Val Asp Leu Leu Lys

465 470 475 480

Arg Arg Gln Asn Gly Leu Leu Leu Leu Ser Val Asp Gly Asn Pro Glu

485 490 495

Leu Cys Ala Leu Asp Ser Cys Asn Asp Asn Asn Lys Lys Asn Tyr Val

500 505 510

Val Pro Val Thr Val Ser Val Ser Ala Leu Phe Ala Ile Val Ala Ala

515 520 525

Ile Ser Leu Ala Ile Cys Leu Lys Arg Arg Lys Lys Gln Ala Ala Val

530 535 540

Ala Gly Glu Met Met Glu Ala Gln Ala Arg Ile Leu Glu Ser Arg Lys

545 550 555 560

Arg Gln Phe Thr Tyr Ser Lys Val Leu Glu Met Thr Asn Asn Phe Glu

565 570 575

Arg Val Leu Gly Arg Gly Gly Phe Gly Thr Val Tyr His Gly Leu Leu

580 585 590

Gly Asp Thr Glu Val Ala Val Lys Thr Leu Ser Pro Ser Ser Val Gln

595 600 605

Gly Tyr Lys Glu Phe Gln Ala Glu Val Lys Leu Leu Leu Arg Val His

610 615 620

His Lys Asn Leu Thr Thr Leu Val Gly Tyr Cys Glu Glu Pro Ser His

625 630 635 640

Met Val Leu Ile Tyr Glu Tyr Met Ala Asn Gly Asp Leu Lys His His

645 650 655

Leu Ser Gly Asp Asp Pro Val Asn Ile Leu Ser Trp Glu Glu Arg Leu

660 665 670

Arg Ile Ala Ile Gly Ala Ala Gln Gly Leu Glu Tyr Leu His Asn Gly

675 680 685

Cys Lys Pro Pro Ile Val His Arg Asp Val Lys Thr Thr Asn Ile Leu

690 695 700

Leu Asp Ser Asn Phe Gln Ala Lys Leu Ala Asp Phe Gly Leu Ser Arg

705 710 715 720

Ile Phe Pro Thr Glu Asp Gly Ser His Val Ser Thr Thr Val Ala Gly

725 730 735

Thr Pro Gly Tyr Leu Asp Pro Glu Tyr His Val Thr Asn Trp Leu Thr

740 745 750

Glu Lys Ser Asp Val Tyr Ser Tyr Gly Val Val Leu Leu Glu Ile Ile

755 760 765

Thr Ser Arg Pro Val Ile Ala Lys Thr Gly Asp Arg Thr His Leu Ser

770 775 780

Thr Trp Val Ser Ser Met Leu Asp Lys Gly Asp Ile Lys Thr Ile Ser

785 790 795 800

Asp Pro Arg Leu Leu Gly Asp Phe Asp Thr Asn Ser Val Trp Lys Val

805 810 815

Thr Glu Leu Ala Met Ala Cys Val Ser Glu Ile Ser Ala Glu Arg Pro

820 825 830

Thr Met Ser Gln Val Leu Val Glu Leu Asn Glu Cys Leu Ala Thr Glu

835 840 845

Thr Ala Arg Thr Arg Glu Gly Arg Ser Gln Ser Asn Ser Asn Ser Ile

850 855 860

Glu Phe Thr Asn Val Asp Leu Ser Ser Gly Leu Phe Pro Gln Pro Arg

865 870 875 880

<210> 14

<211> 849

<212> PRT

<213> Manihot esculenta Crantz

<400> 14

Met Glu Ile Phe Tyr Lys Ser Asp Lys Asp Phe Thr Ser Thr Gly Glu

1 5 10 15

Asn Lys Tyr Val Ser Pro Gly Tyr Asp Asp Asp Asp Asp Ser Ser Tyr

20 25 30

Thr Gly Arg Ile Leu His Ser Leu Arg Phe Phe Pro Lys Gly Arg Lys

35 40 45

Asn Cys Tyr Lys Leu Arg Pro Glu Asn Gly Lys Asn Gln Asn Tyr Leu

50 55 60

Ile Arg Ala Phe Phe Lys Tyr Gly Asn Tyr Asp Ala Gln Tyr Gln Ile

65 70 75 80

Pro Lys Phe Asp Val Tyr Leu Glu Ala Asn Phe Trp Met Thr Val Tyr

85 90 95

Leu Pro Asn Ala Ser Tyr Pro Leu Met Tyr Glu Ile Ile Tyr Phe Ser

100 105 110

Leu Thr Asp Ile Ile Asn Ile Cys Leu Val Asn Thr Leu Ser Gly Thr

115 120 125

Pro Phe Ile Ser Ala Leu Glu Leu Arg Leu Leu Asn Asn Ser Val Tyr

130 135 140

Lys Ile Glu Ser Lys Ser Leu Asn Thr Leu Arg Arg Asp Asp Leu Gly

145 150 155 160

Asn Ser Thr Asn Arg Met Val Arg Tyr Lys Asn Asp Val Tyr Asp Arg

165 170 175

Ile Trp Tyr Thr Ser Ala Ala Thr Asp Tyr Ile Ser Leu Asn Thr Thr

180 185 190

Ser Asn Ile Glu Val Gln Ser Ile Asn Asn Thr Leu Lys Thr Pro Val

195 200 205

Asp Val Leu Arg Thr Ala Leu Gln Leu Arg Ser Pro Leu Arg Ser Leu

210 215 220

Gly Arg Ser Phe Pro Gly Leu Asn Asn Tyr Gly Asn Glu Asn Glu Tyr

225 230 235 240

Tyr Ala Cys Phe His Phe Ala Glu Ile Leu Pro Ile Ser Gln Gly Lys

245 250 255

Gly Lys Arg Pro Arg Glu Phe Thr Ile Asn Phe Asn Gly Ala Asn Tyr

260 265 270

Gly Arg Thr Thr Leu Gln Tyr Leu Asn Pro Leu Thr Thr Cys Tyr Gly

275 280 285

Pro Arg Lys Ser Arg Val Asn Gly Leu Val Asp Phe Phe Ile Asn Gln

290 295 300

Thr Val Arg Ser Asp Leu Pro Pro Ile Leu Asn Ala Phe Glu Leu Phe

305 310 315 320

Tyr Val Met Pro Pro Leu Val Ser Pro Thr Asp Pro Ala Asp Val Asp

325 330 335

Ala Met Thr Val Ile Gln Gln Met Tyr Asn Ile Asn Lys Asp Asp Ser

340 345 350

Trp Gln Gly Asp Pro Cys Leu Pro Arg Asp Tyr Ser Trp Ala Gly Leu

355 360 365

Asn Cys Ser Tyr Asp Thr Asn Ser Pro Arg Ile Ile Ser Leu Asp Leu

370 375 380

Ser Gly Ser Lys Leu Thr Gly Lys Ile Ser Ser Ser Phe Ser Asn Leu

385 390 395 400

Thr Ala Ile Arg Phe Leu Asp Leu Ser Gly Asn Glu Leu Thr Gly Thr

405 410 415

Val Pro Glu Phe Leu Ala Gln Leu Pro Asn Leu Thr Val Leu Asn Leu

420 425 430

Ser Gly Asn Lys Leu Thr Gly Ser Val Pro Gln Ser Leu Val Gln Lys

435 440 445

Ala Asn Asn Arg Leu Leu Gln Leu Ser Leu Glu Gly Asn Pro Ser Leu

450 455 460

Cys Gln Thr Asp Ser Cys Glu Lys Lys Lys His Asn Asn Val Leu Leu

465 470 475 480

Pro Val Val Ile Ser Phe Ala Thr Val Met Val Leu Leu Phe Leu Ser

485 490 495

Ser Ile Phe Phe Phe Trp Arg Met Lys Arg Gln Glu Ala Thr Ser Gln

500 505 510

Ser Lys Lys Glu Gly Leu Val Ile Ser Thr Asn Arg Ser Phe Ser Tyr

515 520 525

Ser Glu Ile Val Ser Ile Thr Asn Asn Phe Glu Thr Ile Ile Gly Glu

530 535 540

Gly Gly Phe Gly Lys Val Tyr Phe Gly Thr Leu Lys Asp Asn Val Gln

545 550 555 560

Val Ala Val Lys Leu Leu Ser Gln Asn Ser Arg Gln Gly Tyr Lys Glu

565 570 575

Phe Gln Ser Glu Ala Gln Leu Leu Met Ile Val His His Arg Asn Leu

580 585 590

Val Ser Leu Ile Gly Tyr Cys Asp Asp Arg His Asn Lys Ala Leu Ile

595 600 605

Tyr Glu Tyr Met Val Asn Gly Asn Leu Arg Glu His Leu Ser Glu Thr

610 615 620

Ser Gly Ser Thr Leu Asn Trp Asn Glu Arg Leu His Ile Ala Ala Asp

625 630 635 640

Ala Ala His Gly Leu Glu Tyr Leu His Asn Gly Cys Lys Pro Pro Ile

645 650 655

Ile His Arg Asp Leu Lys Thr Ser Asn Ile Leu Leu Asn Glu Lys Leu

660 665 670

Arg Ala Lys Ile Ser Asp Phe Gly Leu Ser Arg Ala Phe Ala Asn Glu

675 680 685

Ser Gly Ser His Ile Thr Thr Arg Pro Ala Gly Thr Ile Gly Tyr Leu

690 695 700

Asp Pro Gln Ala Gln Ser Ser Gly Asn Phe Asn Lys Lys Ser Asp Ile

705 710 715 720

Tyr Ser Phe Gly Ile Ile Leu Leu Glu Leu Ile Thr Gly Gln Pro Ala

725 730 735

Ile Arg Arg Asp Val Asn Gly Glu Ile Ile Arg Ile Gln Glu Trp Val

740 745 750

Thr Pro Ile Ile Glu Asn Gly Asp Val Arg Ser Ile Val Asp Pro Arg

755 760 765

Leu Gln Gly Asp Leu Asp Thr Asn Ser Ala Trp Lys Ala Val Glu Ile

770 775 780

Ala Leu Ser Cys Val Leu Asn Thr Ala Thr Arg Arg Pro Asp Met Thr

785 790 795 800

Asp Val Leu Ile Glu Leu Lys Glu Cys Leu Gly Met Val Thr Ala Val

805 810 815

Val Gly Ser Gln Arg Ile Asp Arg Gly Arg Thr Arg Ser Ile Asn Ser

820 825 830

Leu Glu Met Arg Ser Leu Glu Thr Tyr Thr Glu Thr Ala Pro Ser Pro

835 840 845

Arg

<210> 15

<211> 858

<212> PRT

<213> Manihot esculenta Crantz

<400> 15

Met Val Ala Ser Arg Lys Leu Glu Asp Asp Gly Thr Asn His Val Lys

1 5 10 15

Leu Gln Tyr Phe Leu Phe Leu Leu Leu Arg Thr Phe Ala Phe Thr Ala

20 25 30

Val Val Lys Ala Gln Asp Gln Ser Gly Phe Ile Ser Ile Asp Cys Gly

35 40 45

Val Pro Asp Asn Ala Ser Tyr Thr Asp Lys Thr Thr Tyr Leu Asn Tyr

50 55 60

Val Ser Asp Ala Ala Phe Ile Asp Thr Gly Ile Ser Met Lys Ile Ser

65 70 75 80

Pro Glu Phe Arg Thr Tyr Asp Ile Asp Gln Gln Leu Gln Asn Leu Arg

85 90 95

Ser Phe Pro Glu Gly Asn Arg Asn Cys Tyr Asn Val Gly Leu Lys Lys

100 105 110

Gly Ser Lys Tyr Leu Ile Arg Ala Thr Phe Met Tyr Gly Asn Tyr Asp

115 120 125

Gly Gln Asn Lys Ala Pro Gln Phe Asp Ile His Leu Gly Ser Thr Lys

130 135 140

Trp Asp Thr Val Glu Thr Ile Asn Ala Thr Met Ile Ile Thr Lys Glu

145 150 155 160

Ile Ile His Val Thr Thr Met Ser Tyr Leu Leu Ala Cys Leu Ala Asn

165 170 175

Thr Gly Ser Gly Thr Pro Phe Ile Ser Ala Leu Glu Phe Arg Pro Leu

180 185 190

Lys Asn Ala Ser Tyr Asn Tyr Thr Glu Ser Gly Ser Leu Val Arg Phe

195 200 205

Thr Arg Leu Asp Ile Gly Ser Thr Thr Asn Gln Thr Val Arg Tyr Pro

210 215 220

Glu Asp Val Tyr Asp Arg Ile Trp Ala Pro Tyr His Ser Phe Lys Trp

225 230 235 240

Ala Glu Ile Asn Thr Ser Gln Ser Ile Asp Ser Gln Ala Gln Asn Asn

245 250 255

Tyr Arg Pro Pro Ser Val Val Met Arg Thr Ala Gly Thr Pro Ala Asn

260 265 270

Ala Ser Glu Ala Met Thr Leu His Ile Asn Met Glu Asp Asn Thr Leu

275 280 285

Lys Phe Leu Val Tyr Met His Phe Ser Glu Ile Val Arg Leu Gln Ala

290 295 300

Asn Gln Ser Arg Glu Phe Asn Ile Ser Phe Asn Gly Lys His Trp Phe

305 310 315 320

Gly Pro Val Val Pro Asn Tyr Leu Tyr Thr Thr Thr Val Tyr Ser Arg

325 330 335

Ser Ala Leu Thr Ala Gly Gln Tyr Gln Phe Thr Leu Tyr Lys Thr Glu

340 345 350

Cys Ser Thr Leu Pro Pro Leu Leu Asn Ala Ile Glu Ile Tyr Tyr Val

355 360 365

Leu Pro Ile Ser Gln Ser Gln Thr Asn Gln Glu Asp Gly Tyr Leu Trp

370 375 380

Asp Gly Leu Asn Cys Ser Asn Ser Asp Asn Asp Met Leu Arg Ile Ile

385 390 395 400

Ser Leu Asn Leu Ser Ser Ser Gly Leu Thr Gly Glu Ile Thr Arg Ser

405 410 415

Ile Ser Ser Leu Lys Leu Leu Glu Ser Leu Asp Leu Ser Asn Asn Ser

420 425 430

Leu Thr Gly Ser Val Pro Asp Phe Leu Ser Gln Met Ser Ser Leu Lys

435 440 445

Phe Leu Asn Leu Thr Gly Asn Lys Leu Ser Gly Ser Val Pro Thr Ser

450 455 460

Leu Ile Glu Arg Ser Lys Gln Asn Leu Leu Val Leu Ser Lys Lys Asp

465 470 475 480

Lys Asn Trp Val Val Pro Val Val Ala Ser Ile Ala Gly Gly Val Ile

485 490 495

Ala Ile Gly Ala Leu Ala Ile Ile Phe Cys Ser Leu Lys Arg Arg Lys

500 505 510

Gln Lys Val Asp Leu Ala Lys Val Leu Leu Ala Lys Glu Glu Glu Ala

515 520 525

Lys Thr Asn Asn Ala Tyr Glu Ser Leu Lys Ala Lys Gly Arg His Phe

530 535 540

Thr Tyr Ser Glu Ile Leu Ser Ile Thr Asn Asn Phe Glu Arg Val Leu

545 550 555 560

Gly Lys Gly Gly Phe Gly Thr Val Tyr His Gly Tyr Leu Asp Asp Asp

565 570 575

Thr Gln Val Ala Val Lys Met Leu Ser Pro Ser Ser Val Gln Gly Tyr

580 585 590

Arg Gln Phe Gln Ala Glu Val Asn Leu Leu Leu Arg Val His His Arg

595 600 605

Asn Leu Thr Thr Leu Val Gly Phe Cys Asp Glu Gly Thr Asn Arg Gly

610 615 620

Leu Ile Tyr Glu Tyr Met Val Asn Gly Asp Leu Glu Lys Phe Leu Ser

625 630 635 640

Gly Ser Asn Lys Tyr Ile Leu Thr Trp Glu Arg Arg Leu Asn Ile Val

645 650 655

Val Glu Ala Ala Lys Gly Trp Glu Tyr Leu His Asn Gly Ser Lys Pro

660 665 670

Pro Ile Val His Arg Asp Val Lys Thr Ser Asn Ile Leu Leu Asn Asp

675 680 685

Asn Phe Gln Ala Arg Leu Ala Asp Phe Gly Leu Ser Arg Ser Phe Pro

690 695 700

Val Glu Gly Gly Thr His Val Thr Thr Val Val Ala Gly Thr Pro Gly

705 710 715 720

Tyr Leu Asp Pro Glu Tyr Ser Val Thr Asn Trp Leu Thr Glu Lys Ser

725 730 735

Asp Val Tyr Ser Phe Gly Val Val Leu Leu Lys Ile Ile Thr Gly Arg

740 745 750

Pro Val Ile Ala Gly Gly Gly Glu Arg Asn Ile His Leu Arg Gln Trp

755 760 765

Val Ser Ser Leu Leu Ala Asn Gly Asp Ile Lys Asn Val Val Asp Pro

770 775 780

Arg Leu Gly Gly Asp Phe Asp Asn Asn Ser Val Trp Lys Ala Val Glu

785 790 795 800

Ile Ala Met Ala Cys Thr Thr Pro Ile Ser Ala Gly Arg Pro Thr Met

805 810 815

His Gln Val Val Thr Glu Leu Asn Glu Cys Leu Ala Thr Glu Arg Thr

820 825 830

Glu Lys Arg Lys Gly His Ser Arg Ser Glu Pro Ile Ala Ser Ile Glu

835 840 845

Met Ile Thr Glu Ser Thr Pro Leu Ala Arg

850 855

<210> 16

<211> 921

<212> PRT

<213> Manihot esculenta Crantz

<400> 16

Met Asn Met Asp Arg Arg Lys Ala Leu Val Ser Val Ala Pro Trp Leu

1 5 10 15

Ile Phe Ala Leu Leu Tyr Thr Thr Tyr Gly Asn Leu Lys Leu Ala Ala

20 25 30

Ala Ser Asn Asp Asp Val Leu Ala Glu Arg Met Lys Leu Ala Ala Lys

35 40 45

Ser Ser Gly Ser Ile Ser Ile Asp Cys Gly Ala Asp Glu Asp Tyr Val

50 55 60

Asp Glu Asn Val Gly Leu Leu Tyr Lys Ser Asp Arg Asp Phe Ile Ser

65 70 75 80

Thr Gly Lys Asn Tyr Val Ala Gln Pro Asp Ala Thr Arg Leu Gln Ala

85 90 95

Phe Thr Glu Arg Leu Cys Lys Asn Leu Arg Ser Phe Pro Thr Gly Lys

100 105 110

Lys Asn Cys Tyr Asn Leu Arg Pro Glu Gln Gly Lys Asn His Asn Tyr

115 120 125

Tyr Ile Arg Ala Leu Phe Cys Tyr Gly Asn Tyr Asp Gly Lys Asn Gln

130 135 140

Pro Pro Glu Phe Asp Val Tyr Val Gly Val Asn Tyr Trp Val Thr Val

145 150 155 160

Asn Gly Leu Ala Gly Trp Ala Trp Thr Thr Arg Glu Ile Phe His Phe

165 170 175

Ser Ser Thr Asp Val Ile His Val Cys Leu Val Asn Lys Asp Phe Gly

180 185 190

Ile Pro Phe Ile Ser Ala Leu Glu Leu His Pro Val Asn Asp Ser Ile

195 200 205

Tyr Lys Ser Glu Phe Gly Ser Leu Ser Val Ala Arg Arg Ser Gln Leu

210 215 220

Ser Tyr Pro Thr Ala Met Asn Phe Arg Tyr Lys Asp Asp Val Phe His

225 230 235 240

Arg Ile Trp Thr Ser Tyr Gly Leu Ala Asn Ser Thr Trp Val Asn Ala

245 250 255

Ser Ala Thr Ser Asp Met Gln Val His Gln Thr Asp Asp Pro Tyr Lys

260 265 270

Pro Pro Val Glu Ile Phe Arg Thr Ala Ala Gln Pro Gln Lys Gly Leu

275 280 285

His Ser Leu Arg Tyr Asn Ile Ser Phe Ile Cys Ser Pro Ser Cys Asp

290 295 300

Phe Tyr Val Tyr Phe His Phe Ala Glu Ile Val Lys Ile Pro Gln Asp

305 310 315 320

Gln Arg Arg Glu Phe Thr Ile Thr Leu Asn Gly Leu Asn Tyr Gly Pro

325 330 335

Val Ser Leu Glu Tyr Leu Lys Pro Gln Thr Ile Pro Leu Lys Ser Pro

340 345 350

Ala Pro Gly Asp Ile Asn Phe Thr Ile Asn Pro Thr Ser Asn Ser Asp

355 360 365

Leu Pro Pro Ile Leu Ser Ala Phe Glu Ile Leu Ser Val Leu Lys Leu

370 375 380

Pro Leu Ser Pro Thr Asp Gln Ser Asp Val Asp Ala Ile Met Asp Ile

385 390 395 400

Met Gln Leu Tyr Asn Ile Asn Gly Asp Glu Leu Gln Gly Asp Pro Cys

405 410 415

Val Pro Arg Glu Leu Ser Trp Asn Gly Leu Asn Cys Ser Tyr Asp Ser

420 425 430

Asn Pro Pro Arg Ile Ile Ser Leu Asp Leu Ser Ser Arg Lys Leu Thr

435 440 445

Gly Glu Ile Ala Pro Thr Leu Ser Val Leu Lys Ala Ile Gln Ser Leu

450 455 460

Asp Leu Ser Tyr Asn Lys Leu Thr Gly Ser Leu Pro Asn Phe Leu Val

465 470 475 480

Glu Leu Gln Asn Leu Thr Arg Leu Asp Leu Ser Tyr Asn Asp Leu Thr

485 490 495

Gly Pro Leu Pro Glu Phe Leu Ala Gln Leu Pro Asn Leu Asn Thr Leu

500 505 510

Asn Leu Ile Gly Asn Lys Leu Ser Gly Ser Ile Pro Gln Ser Leu Ile

515 520 525

Glu Lys Ser Asn Asn Gly Met Leu Gln Leu Ser Ile Asp Lys Asn Pro

530 535 540

Asn Leu Cys Pro Ser Gly Ser Gly Ser Cys Glu Lys Lys Lys His Phe

545 550 555 560

Leu Leu Pro Val Val Ala Ser Ile Ile Ser Thr Leu Ile Leu Leu Leu

565 570 575

Leu Ile Ala Ile Ser Trp Gly Tyr Arg Arg Lys Lys Glu Thr Gln Asn

580 585 590

Ser Asn Thr Trp Ala Phe Ser Tyr Ser Glu Ile Val Ser Ile Thr Glu

595 600 605

Asp Phe Lys Thr Ile Ile Gly Gly Gly Gly Phe Gly Lys Val Phe Phe

610 615 620

Gly Thr Leu Lys Asp Gly Thr Gln Val Ala Ile Lys Leu Leu Ser Gln

625 630 635 640

Ser Ser Arg Gln Gly Tyr Lys Glu Phe Gln Ala Glu Val Gln Leu Leu

645 650 655

Met Ile Val His His Arg Asn Leu Val Ser Leu Ile Gly Tyr Cys Arg

660 665 670

Asp Ser His Asn Met Ala Leu Val Tyr Glu Phe Met Val Asn Gly Asn

675 680 685

Leu Arg Glu His Leu Ser Glu Thr Ser Gly Ser Ile Leu Thr Trp Lys

690 695 700

Glu Arg Ile Gln Ile Ala Val Asp Ala Ala Tyr Gly Leu Glu Tyr Leu

705 710 715 720

His Asn Gly Cys Lys Pro Pro Ile Ile His Arg Asp Leu Lys Ala Ser

725 730 735

Asn Ile Leu Leu Asn Glu Lys Leu Gln Ala Lys Ile Ala Asp Phe Gly

740 745 750

Leu Ser Arg Ala Phe Thr Ser Glu Ser Gly Thr His Val Ser Thr Cys

755 760 765

Pro Ala Gly Thr Phe Gly Tyr Val Asp Pro Glu Ala Gln Ala Ser Gly

770 775 780

Asn Phe Asn Lys Lys Ser Asp Val Tyr Ser Phe Gly Val Ile Leu Leu

785 790 795 800

Glu Leu Ile Thr Gly Gln Pro Ala Leu Ile Arg Asn Thr Ala Gly Asn

805 810 815

Phe Ile Cys Ile His Gln Trp Ile Arg Pro Ile Ile Asp Thr Gly Asp

820 825 830

Ile Arg Thr Ile Val Asp Ala Arg Leu Gln Gly Gln Phe Asn Val Asn

835 840 845

Ser Ala Trp Glu Val Met Glu Ile Ala Phe Ser Cys Val Ser Asp Val

850 855 860

Ala Ile Gln Arg Pro Asp Met Ser His Val Leu Ala Gly Leu Gln Glu

865 870 875 880

Cys Leu Thr Thr Leu Met Ala Val Glu Ala Ser Gln Lys Leu Glu Ala

885 890 895

Met Thr Thr Arg Ser Ser His Ser Leu Arg Met Arg His Leu Asp Ile

900 905 910

Asp Thr Asp Met Ala Pro Ser Ala Arg

915 920

Claims (4)

1.一种木薯FRK1类似基因在检测病原体侵染情况中应用，所述木薯FRK1类似基因的核苷酸序列如SEQ ID NO.1所示，所述病原体为地毯草黄单胞木薯萎蔫致病变种(Xanthomonas axonopodis pv.manihotis，Xam)和/或粉蚧虫。

2.根据权利要求1所述的应用，其特征在于，所述木薯FRK1类似基因在响应病原体侵染信号中的应用。

3.根据权利要求2所述的应用，其特征在于，所述木薯FRK1类似基因为响应病原体侵染信号的标记基因。

4.根据权利要求3所述的应用，其特征在于，检测病原体侵染情况的方法为检测样品中所述FRK1类似基因表达情况。