CN114277146B - 一种诊断腓骨肌萎缩症的探针组合、试剂盒及应用 - Google Patents
一种诊断腓骨肌萎缩症的探针组合、试剂盒及应用 Download PDFInfo
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Abstract
本发明公开了一种用于诊断腓骨肌萎缩症的探针组合、试剂盒及应用，属于基因检测技术领域。其中，所述探针组合包括靶向PMP22、GJB1、MFN2、MPZ、GDAP1、MORC2、SORD、NEFH、HSPB1、NEFL、PMP2、GLA、PRPS1和TTR中的至少一个基因的至少一个探针。本发明还公开了包含所述探针组合的试剂盒以及在诊断骨肌萎缩症中的应用。本发明的探针组合或试剂盒能够显著提高目标基因变异检测的准确率，并进而有助于腓骨肌萎缩症的精准辅助诊断，具有巨大的临床应用价值。
Description
技术领域
本发明属于基因检测技术领域，具体地，涉及一种诊断腓骨肌萎缩症的探针组合、试剂盒及应用。
背景技术
腓骨肌萎缩症(Charcot-marie-tooth disease，CMT)是一组最常见的，具有高度临床和遗传异质性的遗传性周围神经病，其总体发病率约为1/2500。根据正中神经运动传导速度(Midian motor nerve conduction velocity，MNCV)，CMT可以进一步细分为脱髓鞘型(CMT1，MNCV≤38m/s)，轴索型(CMT2，MNCV>38m/s)和中间型(ICMT，25m/s<MNCV<45m/s)三种。迄今为止，已经有超过100个基因被鉴定和CMT相关，其中：PMP22是CMT1型最常见的基因，其次为MPZ；MFN2是CMT2型最常见的基因而GJB1是CMT中间型最常见基因。据文献报导，PMP22、GJB1、MPZ和MFN2是4种最常见基因，占所有确诊的CMT的90％以上。
尽管全外显子测序在临床上已经被广泛利用，CMT的分子诊断仍然面临着许多挑战：(1)GJB1基因的3′UTR区域的突变也可导致CMT1X表型。(2)PMP22大片段重复突变可以和其他的点突变共同存在，产生共同致病效应(Double trouble effect)，单一检测PMP22基因拷贝数变异或者CMT致病基因的点突变都可能导致漏诊。(3)部分复杂型遗传性周围神经病可以以CMT样表现为唯一症状，包括：转甲状腺素淀粉样多神经病变(Familial amyloidpolyneuropathy，FAP)、遗传性痉挛性截瘫(hereditary spastic paraplegia，HSP)和GNE肌病。
因此，本领域亟需一种能够检测多种与CMT相关基因的变异情况的试剂盒，以辅助CMT精准诊断。
发明内容
为了解决上述技术问题中的至少一个，本发明采用的技术方案如下
本发明第一方面提供一种用于对目标基因进行SNP/Indel变异检测的探针组合的设计方法，包括以下步骤：
S1，将目标基因的每个外显子及其两端50bp作为第一探针区间，针对至少一个所述第一探针区间设计一个或多个探针；
S2，针对目标基因除所述第一探针区间以外的区域设计探针：以100bp为滑动窗口，当窗口中有2个以上致病突变位点时，将该窗口作为第二探针区间，针对至少个所述第二探针区间设计一个或多个探针；
S3，若S1～S2设计的探针GC含量高于80％的，若探针靶向的探针区间具有人群突变频率为20％～80％的SNP位点，则将突变碱基作为探针碱基，若无相关突变位点的，则随机将探针中的5个G或C碱基改变为A或T。
本发明的第三方面提供一种用于对目标基因进行拷贝数变异检测的探针组合的设计方法，针对所述目标基因，每间隔1～3k，设置1个200bp第三探针区间，并针对至少一个所述第三探针区间设计一个或多个CNV-plus探针。
在本发明中，设置步骤S3的原因是：相关基因有较多的基因拷贝数变异(Copynumber variations，CNV)，因此CNV-plus探针用于检测基因CNV。CNV-plus探针在基因上间隔均一，可以有效避免因外显子捕获探针不均一，导致CNV预测不准确，提高基于Reads评估CNV的能力。
在本发明的一些实施方案中，在CNV-plus探针中包含东亚人群突变频率为20％～80％的SNP位点，可用于基于allele balance的CNV分析。在本发明的一些具体实施方案中，设计所述CNV-plus探针的具体步骤如下：
S1’，距离目标基因起始位置1～3k，选取一个200bp的区间，若该区间同时满足(1)包含参考人类基因数据库中人群突变频率为20％～80％的SNP位点，且(2)在基因组上无相似序列，则将该区间作为第一个第三探针区间；
S2’，在选择的第一个第三探针区间之后，在基因上间隔1～3k内再次选取一个200bp的区间，若该区间同时满足S1’中(1)和(2)的条件，则将该区间作为第二个第三探针区间，若距离第一个第三探针区间1～3k内没有符合S1’中(1)和(2)所述要求的区间，则在距离第一个第三探针区间2k位置处选取200bp区间作为第二个第三探针区间，依此类推，直到最后一个第三探针区间后没有同时满足S1’中(1)和(2)的条件的区间且距离基因终止位置不足2k；
S3’，针对得到的至少一个所述第三探针区间设计一个或多个CNV-plus探针。
在本发明的一些实施方案中，所述区间在基因组上无相似序列是指以200bp为滑动窗口在基因组上进行滑动，将每个窗口片段与所述区间进行比对，无90％以上相似度的窗口片段。
在本发明的一些实施方案中，针对本发明第一方面和本发明第二方面，所述目标基因包括但不限于PMP22、GJB1、MFN2、MPZ、GDAP1、MORC2、SORD、NEFH、HSPB1、NEFL、PMP2、GLA、PRPS1、TTR。在这些基因中，有些明确与腓骨肌萎缩症有关，如PMP22基因；有些与其他相关疾病有关，如GLA是Fabry病的致病基因，TTR是遗传性转甲状腺素蛋白淀粉样变性的致病基因；SORD是山梨糖醇脱氢酶缺乏症伴周围神经病的致病基因。对这些与其他相关疾病有关的基因进行变异检测，能够提高诊断腓骨肌萎缩症的准确率和特异性。
本发明第三方面提供一种用于诊断腓骨肌萎缩症的探针组合，包括利用本发明第一方面所述的设计方法对所述腓骨肌萎缩症及其相关疾病相关基因进行设计得到的探针组合，所述腓骨肌萎缩症及其相关疾病相关基因选自PMP22、GJB1、MFN2、MPZ、GDAP1、MORC2、SORD、NEFH、HSPB1、NEFL、PMP2、GLA、PRPS1、TTR中的至少一种。
在本发明的一些实施方案中，靶向PMP22基因的探针组合包括SEQ ID No.87～129所示的探针；靶向GJB1基因的探针组合包括SEQ ID No.388～401所示的探针；靶向MFN2基因的探针组合包括SEQ ID No.1～10所示的探针；靶向MPZ基因的探针组合包括SEQ IDNo.11～20所示的探针；靶向GDAP1基因的探针组合包括SEQ ID No.291～322所示的探针；靶向MORC2基因的探针组合包括SEQ ID No.220～241所示的探针；靶向SORD基因的探针组合包括SEQ ID No.21～40所示的探针；靶向NEFH基因的探针组合包括SEQ ID No.170～219所示的探针；靶向HSPB1基因的探针组合包括SEQ ID No.242～257所示的探针；靶向NEFL基因的探针组合包括SEQ ID No.258～290所示的探针；靶向PMP2基因的探针组合包括SEQ IDNo.323～339所示的探针；靶向GLA基因的探针组合包括SEQ ID No.340～368所示的探针；靶向PRPS1基因的探针组合包括SEQ ID No.369～387所示的探针；靶向TTR基因的探针组合包括SEQ ID No.151～169所示的探针。
在本发明的一些实施方案中，所述用于诊断腓骨肌萎缩症的探针组合还包括用于分析PMP22基因拷贝数变异的探针组合，所述用于分析PMP22基因拷贝数变异的探针组合利用本发明第二方面所述的设计方法设计得到。
在本发明的一些方案中，所述用于分析PMP22基因拷贝数变异的探针组合包括靶向chr17:14100991-14101127、chr17:14133516-14133640、chr17:14171563-14171695、chr17:14235680-14235802、chr17:14285014-14285146、chr17:14312221-14312359、chr17:14343889-14344015、chr17:14409890-14410024、chr17:14457954-14458086、chr17:14492312-14492444、chr17:14531113-14531265、chr17:14596178-14596308、chr17:14646877-14646999、chr17:14655667-14655843、chr17:14735456-14735580、chr17:14780118-14780242、chr17:14800953-14801085、chr17:14850608-14850734、chr17:14898767-14898911、chr17:14939853-14939975、chr17:14979111-14979257、chr17:15018350-15018500、chr17:15065290-15065416、chr17:15133696-15133826、chr17:15136057-15136181、chr17:15141300-15141426、chr17:15142280-15142454、chr17:15144794-15144916、chr17:15148220-15148342、chr17:15150538-15150664、chr17:15152985-15153107、chr17:15155327-15155469、chr17:15157779-15157901、chr17:15158695-15158833中的至少一个区间的一个或多个探针。
在本发明的一些实施方案中，靶向chr17:14100991-14101127的探针组合包括SEQID No.41～42所示的探针；靶向chr17:14133516-14133640的探针组合包括SEQ ID No.43～44所示的探针；靶向chr17:14171563-14171695的探针组合包括SEQ ID No.45～46所示的探针；靶向chr17:14235680-14235802的探针组合包括SEQ ID No.47～48所示的探针；靶向chr17:14285014-14285146的探针组合包括SEQ ID No.49～50所示的探针；靶向chr17:14312221-14312359的探针组合包括SEQ ID No.51～52所示的探针；靶向chr17:14343889-14344015的探针组合包括SEQ ID No.53～54所示的探针；靶向chr17:14409890-14410024的探针组合包括SEQ ID No.55～56所示的探针；靶向chr17:14457954-14458086的探针组合包括SEQ ID No.57～58所示的探针；靶向chr17:14492312-14492444的探针组合包括SEQID No.59～60所示的探针；靶向chr17:14531113-14531265的探针组合包括SEQ ID No.61～62所示的探针；靶向chr17:14596178-14596308的探针组合包括SEQ ID No.63～64所示的探针；靶向chr17:14646877-14646999的探针组合包括SEQ ID No.65～66所示的探针；靶向chr17:14655667-14655843的探针组合包括SEQ ID No.67～68所示的探针；靶向chr17:14735456-14735580的探针组合包括SEQ ID No.69～70所示的探针；靶向chr17:14780118-14780242的探针组合包括SEQ ID No.71～72所示的探针；靶向chr17:14800953-14801085的探针组合包括SEQ ID No.73～74所示的探针；靶向chr17:14850608-14850734的探针组合包括SEQ ID No.75～76所示的探针；靶向chr17:14898767-14898911的探针组合包括SEQID No.77～78所示的探针；靶向chr17:14939853-14939975的探针组合包括SEQ ID No.79～80所示的探针；靶向chr17:14979111-14979257的探针组合包括SEQ ID No.81～82所示的探针；靶向chr17:15018350-15018500的探针组合包括SEQ ID No.83～84所示的探针；靶向chr17:15065290-15065416的探针组合包括SEQ ID No.85～86所示的探针；靶向chr17:15133696-15133826的探针组合包括SEQ ID No.130～131所示的探针；靶向chr17:15136057-15136181的探针组合包括SEQ ID No.132～133所示的探针；靶向chr17:15141300-15141426的探针组合包括SEQ ID No.134～135所示的探针；靶向chr17:15142280-15142454的探针组合包括SEQ ID No.136～137所示的探针；靶向chr17:15144794-15144916的探针组合包括SEQ ID No.138～139所示的探针；靶向chr17:15148220-15148342的探针组合包括SEQ ID No.140～141所示的探针；靶向chr17:15150538-15150664的探针组合包括SEQ ID No.142～143所示的探针；靶向chr17:15152985-15153107的探针组合包括SEQ ID No.144～145所示的探针；靶向chr17:15155327-15155469的探针组合包括SEQ ID No.146～147所示的探针；靶向chr17:15157779-15157901的探针组合包括SEQ ID No.148～149所示的探针；靶向chr17:15158695-15158833的探针组合包括SEQ ID No.150所示的探针。
本发明第四方面提供一种用于诊断腓骨肌萎缩症相关基因进行变异检测的试剂盒，所述试剂盒包括本发明第三方面任一所述的探针组合。
在本发明的一些实施方案中，所述用于诊断腓骨肌萎缩症的试剂盒还包括基因组DNA提取试剂、末端修复试剂、末端加“A”试剂、核酸扩增试剂和/或纯化试剂。
本发明第四方面提供一种本发明第三方面任一所述的探针组合在制备适用于以下方法诊断腓骨肌萎缩症的试剂盒中的应用，所述方法包括以下步骤：
S101，获得个体的基因组DNA样本；
S102，利用所述探针组合进行富集并进行高通量测序；
S103，根据高通量测序数据诊断个体是否患有腓骨肌萎缩症。
在本发明的，可以利用任意高通量测序平台进行高通量测序。
在本发明的一些实施方案中，所述变异包括SNP/Indel变异以及CNV变异。
在本发明的一些实施方案中，利用以下步骤进行SNP/Indel分析：
对高通量测序数据进行质控，得到clean reads，利用基因比对软件将cleanreads比对到参考基因组上，得到最初比对结果，优选地，对比对结果任选地经过包括但不限于去重复、局部重比对、碱基质量值样本校正处理，得到最终比对结果，其中，如果一个或一对read(s)在基因上可以有多个比对位置，则从中选择一个最好(MQ值最高)的，如果有两个或以上最好的比对位置，则从中随机选择一个；进一步地，根据最终比对结果进行SNP/Indel分析。
在本发明的一些实施方案中，利用以下步骤进行CNV分析：
任选地，利用reads进行CNV分析，具体地，利用reads深度(reads depth)进行CNV分析；任选地，利用allele balance进行CNV分析；任选地，综合利用reads进行CNV分析的结果和利用allele balance进行CNV分析的结果。
在本发明的一些实施方案中，所述诊断为辅助诊断，需要结合人体其他临床表征或检测数据由专业医生进行最终判断。
本发明的有益效果
相对于现有技术，本发明具有以下有益效果：
本发明的探针设计方法不仅针对目标基因的外显子进行设计，还针对外显子两端50bp进行设计，并且还针对目标基因的非编码区域尤其是基因调控区进行探针设计，相较于只对编码区捕获的探针设计更为全面，能够显著提高目标基因变异检测的精准度，并进而助力疾病精准辅助诊断。
根据本发明的探针设计方法，设计一个包含所有已知CMT致病基因和部分常见复杂性周围神经病致病基因的的探针组合，能良好覆盖GJB1基因的非编码区域，并且能够检测常见基因如PMP22基因拷贝数变异，提高CMT精准诊断率，减轻患者的时间和经济负担。
通常由于探针捕获的波动性与人为操作、仪器波动、试剂批次等因素影响较为密切，不能保证每个批次的结果的波动在一定范围内，会不定向偏差。本发明提出了allelebalance的CNV分析方法，通常使用高通量捕获的突变其突变丰度在一个恒定的范围内，并不会由于人为影响导致其有较大的变化。
具体实施方式
除非另有说明、从上下文暗示或属于现有技术的惯例，否则本申请中所有的份数和百分比都基于重量，且所用的测试和表征方法都是与本申请的提交日期同步的。在适用的情况下，本申请中涉及的任何专利、专利申请或公开的内容全部结合于此作为参考，且其等价的同族专利也引入作为参考。如果现有技术中披露的具体术语的定义与本申请中提供的任何定义不一致，则以本申请中提供的术语定义为准。
术语“包含”，“包括”，“具有”以及它们的派生词不排除任何其它的组分、步骤或过程的存在，且与这些其它的组分、步骤或过程是否在本申请中披露无关。为消除任何疑问，除非明确说明，否则本申请中所有使用术语“包含”，“包括”，或“具有”的组合物可以包含任何附加的添加剂、辅料或化合物。相反，出来对操作性能所必要的那些，术语“基本上由……组成”将任何其他组分、步骤或过程排除在任何该术语下文叙述的范围之外。术语“由……组成”不包括未具体描述或列出的任何组分、步骤或过程。除非明确说明，否则术语“或”指列出的单独成员或其任何组合。
为了使本发明所解决的技术问题、技术方案及有益效果更加清楚明白，以下结合实施例，对本发明进行进一步详细说明。
实施例
以下例子在此用于示范本发明的优选实施方案。本领域内的技术人员会明白，下述例子中披露的技术代表发明人发现的可以用于实施本发明的技术，因此可以视为实施本发明的优选方案。但是本领域内的技术人员根据本说明书应该明白，这里所公开的特定实施例可以做很多修改，仍然能得到相同的或者类似的结果，而非背离本发明的精神或范围。
除非另有定义，所有在此使用的技术和科学的术语，和本发明所属领域内的技术人员所通常理解的意思相同，在此公开引用及他们引用的材料都将以引用的方式被并入。
那些本领域内的技术人员将意识到或者通过常规试验就能了解许多这里所描述的发明的特定实施方案的许多等同技术。这些等同将被包含在权利要求书中。
下述实施例中未作具体说明的分子生物学实验方法，均按照《分子克隆实验指南》(第四版)(J.萨姆布鲁克、M.R.格林，2017)一书中所列的具体方法进行，或者按照试剂盒和产品说明书进行。其他实验方法，如无特殊说明，均为常规方法。下述实施例中所用的仪器设备，如无特殊说明，均为实验室常规仪器设备；下述实施例中所用的试验材料，如无特殊说明，均为自常规生化试剂商店购买得到的。
实施例1探针设计
本实施例利用以下方法设计CMT相关基因检测的特异性探针：
1)探针覆盖外显子区域及和外显子两端50bp；
2)针对外显子以外的调控区域，以100bp为滑动窗口，当一个窗口中有2个及2个以上已知致病突变时，保留该区间，并设计相关探针；
3)探针设计使用叠瓦式，参考区间中的每个碱基至少有3条以上探针对其进行覆盖；
4)针对存在CNV的目标基因，每间隔1～3k，增加1个200bp CNV-plus探针区间，该探针区间需包含gnomad人群数据库中，东亚人群突变频率为20％～80％的SNP位点且该区间在基因组上无相似序列(以200bp为滑动窗口，进行全基因组比对，无90％以上相似度的序列)，如间隔1～3k内无符合要求的SNP位点，则取距离上个最近区间2k位置设计一个200bp大小的区间，并设计探针；
5)对于设计探针GC含量高于80％的，如探针区域有人群突变频率为20％～80％的SNP位点的将突变碱基作为探针碱基，如无相关突变位点的，随机将探针中的5个G或C碱基改变为A或T。
根据以上设计原则，发明人针对PMP22、GJB1、MFN2、MPZ、GDAP1、MORC2、SORD、NEFH、HSPB1、NEFL、PMP2、GLA、PRPS1、TTR基因设计探针，并针对PMP22基因设计CNV-plus探针，得到靶向以下区间的探针：
chr17:14100991-14101127、chr17:14133516-14133640、chr17:14171563-14171695、chr17:14235680-14235802、chr17:14285014-14285146、chr17:14312221-14312359、chr17:14343889-14344015、chr17:14409890-14410024、chr17:14457954-14458086、chr17:14492312-14492444、chr17:14531113-14531265、chr17:14596178-14596308、chr17:14646877-14646999、chr17:14655667-14655843、chr17:14735456-14735580、chr17:14780118-14780242、chr17:14800953-14801085、chr17:14850608-14850734、chr17:14898767-14898911、chr17:14939853-14939975、chr17:14979111-14979257、chr17:15018350-15018500、chr17:15065290-15065416、chr17:15133696-15133826、chr17:15136057-15136181、chr17:15141300-15141426、chr17:15142280-15142454、chr17:15144794-15144916、chr17:15148220-15148342、chr17:15150538-15150664、chr17:15152985-15153107、chr17:15155327-15155469、chr17:15157779-15157901、chr17:15158695-15158833
由此，设计探针序列如表1所示(基因顺序与前述不同，但本领域知悉，基因的顺序的描述不影响本发明公开的实质内容，不影响本发明的实际保护范围)。
表1探针信息
注：探针名称中“_”后面的内容表示探针靶向的基因或者片段，如“15981_PMP22”表示该探针靶向PMP22基因，“15989_chr17:15148220-15148342”表示该探针靶向chr17:15148220-15148342片段。
实施例2目标区域捕获测序
本实施例使用了实施例1提供捕获探针组，并且基于液相杂交的序列捕获技术对靶序列进行富集。液相杂交技术路线是：(1)制备杂交探针库，(2)使用探针对目标基因进行富集，(3)将富集后的DNA序列用高通量测序仪进行测序。
使用液相杂交技术能对目标基因进行有效富集，降低样本量的要求，同时也能更好地控制测序成本。该文库构建方法包括如下步骤：
1.DNA提取和打断
使用QIAamp DNA Blood Mini Kit提取4个外周血样本中DNA。
使用Bioruptor Pico DNA打断仪，待冷循环仪温度降至4℃后，设置参数ON 30s，OFF 30s为1个循环，每10循环为一轮，共进行3轮，每组结束后将样品置于振荡器上充分混匀，短暂离心后进行下一轮打断。
取1μL样品使用QSEP 100进行片段检测，正常打断后样品检测主峰约在150bp-200bp。
2.末端修复
预先从-20℃保存的试剂盒中取出10×T4 PNK Buffer和Natural dNTP Mix，将其置于冰上融化并用Vortex充分混匀直至Buffer中没有任何固体不溶物，将酶从-20℃冰箱取出置于-20℃冰盒上。
在1.5mL的离心管中分别配制末端修复反应体系，如表2所示。
表2末端修复反应体系
| 试剂 | 体积/μL |
| 来自1的样品 | 41 |
| 10×T4 PNK Buffer | 5 |
| 10mM dNTP Mix | 1 |
| T4 DNA Polymerase | 1 |
| T4 PNK | 1 |
| Klenow Fragment | 1 |
| 共计 | 100 |
20℃温浴30min。反应后产物用Ampure XP磁珠进行纯化，溶解于20μL TE中。
3.末端加“A”(A-Tailing)
在1.5mL的离心管中分别配制末端加“A”反应体系，如表3所示。
表3末端加“A”体系
| 试剂 | 体积/μL |
| 来自2的样品 | 19.5 |
| 10×Blue Buffer | 2.5 |
| 1mM dATP | 2.5 |
| Klenow(3’-5’exo-) | 0.5 |
| 共计 | 25 |
37℃温浴30min。反应后产物使用Ampure XP磁珠进行纯化，溶解于25μL TE中。
4.接头(Adapter)连接
在1.5mL的离心管中配制Adapters连接反应体系，如表4所示。
表4 Adapter连接体系
20℃温浴15min，反应后产物使用Ampure XP磁珠进行纯化，溶解于21μL TE中。
5.PCR扩增加Index
增加Index的PCR反应体系及反应条件如表5所示：
表5加Index的PCR反应体系
| 试剂 | 体积/μL |
| 来自步骤4的样品 | 21 |
| HiFi Mix | 25 |
| Index primers 1 | 2 |
| Index primers 2 | 2 |
| Total | 50 |
在PCR仪中运行下列程序：
反应后产物使用Ampure XP磁珠进行纯化。溶解于50μL TE中。使用NanoDrop1000检测PCR产物浓度。使用Qubit 3.0进行文库定量，文库浓度>25ng/μL参考为合格文库。使用QSEP 100检测，文库主峰要在220-320bp左右，主峰前后无杂峰。
6.杂交
将Hyb Block及Hyb Buffer从冰箱取出，冰上融化，融化后将Hyb Buffer置于金属浴上65℃预热。
按照表6体系将样品文库与Hyb block混匀，标记为B。
表6杂交体系
| 组分 | 用量 |
| 样品文库 | 600ng |
| Hyb Block | 5μL |
将Hyb Buffer置于室温融化，未加热前会有沉淀出现，混匀后置于65℃水浴锅内预热，完全溶解后(无沉淀及浑浊物)取20μL Hyb Buffer置于新的200μL PCR管内，盖好管盖，标记为A，继续置于65℃水浴锅内孵育待用。
取5μL RNase Block与2μL Probe置于200μL PCR管内，轻轻吸打混匀，短暂离心后置于冰上待用，标记为C。
设置PCR仪参数，heat lid 100℃，95℃，5min；65℃，hold；
将PCR管B置于PCR仪上，运行以上程序。
PCR仪温度降至65℃时，将PCR管A置于PCR仪上孵育，盖上PCR仪热盖；5min后，将C置于PCR上孵育，盖上PCR仪热盖；将PCR管C放置入PCR仪2min后，把移液器调至13μL，从PCR管A中吸取13μL Hyb Buffer移至PCR管C中，吸取全部PCR管B中样品移至PCR管C中，轻轻吸打10次，充分混匀，避免产生大量气泡，密封管盖，盖上PCR仪热盖，65℃孵育过夜(8-16h)。
7.捕获
提前分装Wash Buffer 2(每个捕获需要1.8mL)，并置于ThermoMixer上65℃预热。
保持杂交产物PCR管C在PCR仪上，将杂交后PCR管C的产物加入到200μLBiotin-bed中，用移液器吸打6次混匀，置于旋转混匀仪上(10rpm/min)室温结合30min后将离心管置于磁力架上2min，然后移除上清液。
向离心管内加入500μL的Wash Buffer 1，轻轻吸打6次混匀，重悬磁珠，并于漩涡混合仪上振荡5s混匀样品，室温下孵育样品15min。
加入500μL的65℃预热的Wash Buffer 2，涡旋混匀5s，置于ThermoMixer上65℃孵育10min，转速800转/min进行清洗。
短暂离心，将离心管放于磁力架上2min，移除上清。重复洗2次清洗，共计3次。最后一次彻底移除除Wash Buffer2(可用10μL移液器移除残留)。
向离心管中加入25μL Nuclease-free water，从磁力架上取下离心管，轻轻吸打6次重悬磁珠待用。
8.富集
获后需要对DNA文库进行富集，根据表7配制混合物(Mix)。
表7富集Mix体系
PCR扩增体系如下：
将PCR产物纯化后，文库使用Qubit dsDNA HS Assay Kit进行定量，使用QSEP 100进行文库片段长度测定，文库长度约在220-320bp之间。捕获好的文库通过高通量测序仪(Illumina NovaSeq 6000system)进行测序。
9.测序数据分析
9.1数据质控
测序得到的原始图像数据经basecalling转化为序列数据，即为raw data或rawreads，结果以fastq文件格式存储(文件名:*.fq)。Raw data中会包含接头信息，低质量碱基，未测出的碱基(以N表示)，这些信息会对后续的信息分析造成很大的干扰，分析前需要将这些干扰信息去除掉，最终得到的数据即为有效数据，即为clean data或clean reads。原始数据过滤方法如下：
(1)过滤掉含有接头序列的reads；
(2)当单端测序read中含有的N的含量超过该条read长度比例的10％时，需要去除此对paired reads；
(3)当单端测序read中含有的低质量(碱基质量值小于5)碱基数超过该条read长度比例的50％时，需要去除此对pairedreads。经过对测序数据的严格过滤，得到高质量的cleandata。对产出数据进行统计，包括测序read数量，数据产量，测序错误率，Q20含量，Q30含量，GC含量等。
9.2比对
有效测序数据通过BWA比对到参考基因组(UCSC hg19)，得到BAM格式的最初的比对结果。BAM文件再用Picard和GATK进行去重复(duplicate removal)、局部重比对(localrealignment)、碱基质量值重校正(basequality recalibration)等处理，从而得到BAM格式的最终比对结果。如果一个或一对read(s)在基因上可以有多个比对位置，BWA的处理策略是从中选择一个最好的(MQ值最高的)，如果有两个或以上“最好”的比对位置，则从中随机选择一个。这种多重比对(multiple hit)的处理对SNP、Indel以及CNV等的检测有重要影响。通常检测SNP或Indel的时候要使用高质量的比对(alignment)。
9.3 Indel变异检测
最终的BAM文件用GATK进行SNP/Indel(小于50bp的小片段)的检测。
9.4基因的CNV变异检测
9.4.1利用reads进行CNV分析
评估bed区间的GC含量、捕获效率。对低捕获效率的目标区间(对区间平均深度进行排序，位于末位百分之五的区间)进行过滤，不加入CNV评估的区间分析。
标准化数据量：每次检测样本，计算捕获区间reads数，当reads数是正常对照样本的70％～130％则进行下一步分析。如reads数大于130％，则使用seqtk抽取相应数据量数据后，进行进一步分析。
reads depth判定：使用由上述步骤9.2比对产生的BAM文件，统计所有待分析样本无相似序列bed区间内的reads数目。
利用贝塔二项分布来描述特定区域正常样本和对照样本的覆盖度比值，用隐马尔可夫模型来预测CNV情况。
9.4.2利用allele balance进行CNV分析
针对每个基因选取目标区域内的所有SNP。对SNP进行以下过滤：保留非基因组重复区域及基因座控制区(Locus control region，LCR)的单核苷酸突变，GQ>＝20，DP>＝10，allele balance>0.2的SNP。
计算筛选出样本的杂合度：杂合SNP数量/总过滤后SNP数量。当杂合度<0.1，判定为del；如杂合度≥0.1，删除allele balanc≥0.9的SNP，对剩余SNP的allele balance根据表8计算得分(Score)：
表8根据allele balance计算得分
对所有位点的SNP求和：获得∑Score，进行表9所示判定：其中使用SNP数量作为主要质控参数，当SNP数量不满足需求时，作为未知(unkonw)。
表9根据SNP判定
9.4.3CNV复核校验
根据Reads depth判定结果及allele balance∑Score进行综合判定，如表10所示。
表10综合判定结果
| ∑Score判定结果 | Reads depth判定结果 | 综合判定结果 |
| Dup | Dup | Dup+ |
| Dup | Normal | Dup |
| Dup | Del | Unkonw |
| Normal | Dup | Normal |
| Normal | Normal | Normal |
| Normal | Del | Normal |
| Del | Dup | Unkonw |
| Del | Normal | Del |
| Del | Del | Del+ |
| Unkonw | Dup | Dup- |
| Unkonw | Normal | Normal |
| Unkonw | Del | Del- |
实施例3样本检测
选取20例样本利用实施例2所示方法进行检测，同时使用WES、MLPA对样本的PMP22基因CNV进行平行比较实验。WES使用试剂盒为：SureSelect人全外显子V7外显子组；MLPA使用试剂盒为：MRC-Holland P405检测试剂盒。
结果如表11所示，显示实施例2方法检测结果与MLPA符合率为100％，并且高于单纯基于Reads depth分析的结果。
表11样本检测结果
在本发明提及的所有文献都在本申请中引用作为参考，就如同每一篇文献被单独引用作为参考那样。此外应理解，在阅读了本发明的上述讲授内容之后，本领域技术人员可以对本发明作各种改动或修改，这些等价形式同样落于本申请所附权利要求书所限定的范围。
序列表
<110> 中南大学湘雅三医院
杭州祥音医学检验实验室有限公司
<120> 一种诊断腓骨肌萎缩症的探针组合、试剂盒及应用
<130> XYY202111831
<140> 202111625291X
<141> 2021-12-28
<160> 401
<170> SIPOSequenceListing 1.0
<210> 1
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1
gaagcgagga tcaactggtt gcagtgacct gagatcccca tattcttgat tctccccaaa 60
gcattccgcc atctccctag caggatgtcc cgaggtaggt gttgctgggt tcgctcattg 120
cgtgaaccga 130
<210> 2
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 2
gaagcgagga tcaactaggt gttgctgggt tcgctcacgt tagcttctct tgcagcgcaa 60
tgtccctgct cttctctcga tgcaactcta tcgtcacagt caagaaaaat aagagcattg 120
cgtgaaccga 130
<210> 3
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 3
gaagcgagga tcaactcagt caagaaaaat aagagacaca tggctgaggt gaatgcatcc 60
ccacttaagc actttgtcac tgccaagaag aagatcaatg gcatttttga gcagccattg 120
cgtgaaccga 130
<210> 4
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 4
gaagcgagga tcaactaatg gcatttttga gcagctgggg gcctacatcc aggagagcgc 60
caccttcctt gaaggtaagg gggcaccggc tcagccaggc ccgctcttac ctgttcattg 120
cgtgaaccga 130
<210> 5
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 5
gaagcgagga tcaactaggc ccgctcttac ctgtttagat atttagctag tgggatttgc 60
gggtggggag gtatatctct gctcccaggg aagtcataac cagatagcct tagaacattg 120
cgtgaaccga 130
<210> 6
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 6
gaagcgagga tcaacttctg gagctcagcc tgtcggctgc agggtgccag aggtggactc 60
gtttagggta agcagggccg gcgctctggc ccttccagac ttgggactgt ggaaccattg 120
cgtgaaccga 130
<210> 7
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 7
gaagcgagga tcaactagac ttgggactgt ggaactcctc tgaccacgtg gtgacccatt 60
ttcaatcccc acctccagac acgtacagga atgcagaact ggaccccgtt accaccattg 120
cgtgaaccga 130
<210> 8
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 8
gaagcgagga tcaactaact ggaccccgtt accacagaag aacaggttct ggacgtcaaa 60
ggttacctat ccaaagtgag aggcatcagt gaggtgctgg ctcggaggca catgacattg 120
cgtgaaccga 130
<210> 9
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 9
gaagcgagga tcaactctgg ctcggaggca catgaaagtg gctttttttg gccggtaagt 60
ccttgaggca cccacccttt ctttcttcct ggctaggagg gagggaggca ctttgcattg 120
cgtgaaccga 130
<210> 11
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 11
gaagcgagga tcaactgagg gagggaggca ctttgtgcaa ggtcacagaa cgttccaggc 60
agggacatgc ttcagaattc attgctttcc tcttaattta tattttattt atttacattg 120
cgtgaaccga 130
<210> 12
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 12
gaagcgagga tcaactactt gacagcaggc cggagctccg ggctctgctc atcctttcgt 60
agctccatct cgatgaccat cacctttggg cctttggcgg actccacccc taacccattg 120
cgtgaaccga 130
<210> 13
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 13
gaagcgagga tcaactgcgg actccacccc taacccccga tcccccgccc ggcccgctaa 60
ccgctatttc ttatccttgc gagactcccc cagccccttg gccttcttct cactgcattg 120
cgtgaaccga 130
<210> 13
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 13
gaagcgagga tcaactcttg gccttcttct cactgacagc tttggtgctt ctgctgtggt 60
ccagcattgc atacagcact ggcgtctggg ggaggggcgc acacatcagt caccgcattg 120
cgtgaaccga 130
<210> 14
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 14
gaagcgagga tcaactgcgc acacatcagt caccgagcga ctggggcttg actgttccca 60
tcccacccct cactgctgcc cggcggctcc cagggttctc cttcccatct tgtctcattg 120
cgtgaaccga 130
<210> 15
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 15
gaagcgagga tcaacttctc cttcccatct tgtctaggcc ccaagtcccg ctaacctgcc 60
gcccgcgctt cgacgcgtcc tttcctggct tgtgcaattt ccccttctcc atagccattg 120
cgtgaaccga 130
<210> 16
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 16
gaagcgagga tcaactattt ccccttctcc atagcactgc aagaagagag actgctgtac 60
gtttggcctc gccggaaccc cttgcaccgc ggacacagct tcctcttccc cttggcattg 120
cgtgaaccga 130
<210> 17
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 17
gaagcgagga tcaactagct tcctcttccc cttggccctc ccacccactg gagtagtctc 60
cgcccagatg ggggatagtg gggagagggg gaggggagct aggctccgcc ccttacattg 120
cgtgaaccga 130
<210> 18
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 18
gaagcgagga tcaactagct aggctccgcc ccttacctga gcctcctctg cagggccgcc 60
tgcctgcgta gccagcagta ccgaaccacg tagaaaagca gcagcagcaa cagcacattg 120
cgtgaaccga 130
<210> 19
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 19
gaagcgagga tcaactagca gcagcagcaa cagcaccacc ccgaggacac ccccgatcac 60
agctcccaga acgaccccgt acctagttgg cactaggagg ggtgggaaaa gaagtcattg 120
cgtgaaccga 130
<210> 20
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 20
gaagcgagga tcaactgagg ggtgggaaaa gaagtgggag aatgagcagg gccctgtatc 60
tgtggttcct agtccgagtg tatgccctgc attgaggatg taggactccc agctacattg 120
cgtgaaccga 130
<210> 21
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 21
gaagcgagga tcaactagca aggtccttcg acttatgtgt tcattcaaca taaatatgtg 60
tgcatgcttt ctttgggcca ggccctctgt taagctctgg gtacagcagg gaacacattg 120
cgtgaaccga 130
<210> 22
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 22
gaagcgagga tcaactagca tgcaagcctt cataacatct ctgcttctgc tgttttcaaa 60
ggttggaaaa caaagaacca gaccaaagag cgggcagact ctgctcccac cctcgcattg 120
cgtgaaccga 130
<210> 23
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 23
gaagcgagga tcaactgact ctgctcccac cctcggacat ggtgccatct ttgttttcct 60
ctccaggtga tcgtgttgcc atcgagcctg gtgctccccg agaaaatgat gaattcattg 120
cgtgaaccga 130
<210> 24
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 24
gaagcgagga tcaactcccg agaaaatgat gaattctgca agatgggccg atacaatctg 60
tcaccttcca tcttcttctg tgccacgccc cccgatgacg ggaacctctg ccggtcattg 120
cgtgaaccga 130
<210> 25
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 25
gaagcgagga tcaactgacg ggaacctctg ccggttctat aagcacaatg cagccttttg 60
ttacaagtta gtgtccacag tcccactggg tcacctggga cctctttccc ttcatcattg 120
cgtgaaccga 130
<210> 26
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 26
gaagcgagga tcaactggga cctctttccc ttcattagct tggtgctgtt tcctgtggtt 60
atttctttat tcttttcagc tcataattcc aaacatgagg catcacctgg acaggcattg 120
cgtgaaccga 130
<210> 27
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 27
gaagcgagga tcaacttata aattgcttag cacattgctc tgcacataaa taaatgctcg 60
ataaatgcta gctatggttg ttattacagt gacattgggc atatataata ctattcattg 120
cgtgaaccga 130
<210> 28
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 28
gaagcgagga tcaactcaat gtcacctttg aggaaggcgc cctgatcgag ccactttctg 60
tggggatcca tgcctgcagg agaggcggag ttaccctggg acacaaggtc cttgtcattg 120
cgtgaaccga 130
<210> 29
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 29
gaagcgagga tcaacttggg acacaaggtc cttgtgtgtg gagctggtaa gaaacagaag 60
ccaccctgtt gcgggttcat tgactgggaa ttcagggaac cctctgccca tctcacattg 120
cgtgaaccga 130
<210> 30
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 30
gaagcgagga tcaactgaac cctctgccca tctcattccc cctccaaggc ttgagatttg 60
ttctagaatc cttctgggta agggaggatt gcagtgtccc attccctcag tgactcattg 120
cgtgaaccga 130
<210> 31
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 31
gaagcgagga tcaactctaa ggtcttatca tgccaggcac tgggatagat gctagagaca 60
cacaaaagat atggttccta tccatggcct ggacaagtgg gagagcagat gtttacattg 120
cgtgaaccga 130
<210> 32
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 32
gaagcgagga tcaactgtgg gagagcagat gtttaatatt tcacgaacat attccatctt 60
ctgctttgtt tagggccaat cgggatggtc actttgctcg tggccaaagc aatggcattg 120
cgtgaaccga 130
<210> 33
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 33
gaagcgagga tcaactctcg tggccaaagc aatgggagca gctcaagtag tggtgactgg 60
taagactttg ttctttatca atctccttga ctgggaaaca gcgggtccta ctgtacattg 120
cgtgaaccga 130
<210> 34
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 34
gaagcgagga tcaactaaca gcgggtccta ctgtatgtgc atgtgtgagg agaggttatc 60
tgtgacagtg tggagggggt gagtgtgtag tgtcatatgg attgtggaaa tatagcattg 120
cgtgaaccga 130
<210> 35
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 35
gaagcgagga tcaactcacc cattgcacga gagctttgtt gccatcagat cttacatctc 60
cattttcttt tcttcctaat gagtcatcag atttctcttg tttgaaagaa tttttcattg 120
cgtgaaccga 130
<210> 36
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 36
gaagcgagga tcaactcttg tttgaaagaa tttttttttt tttttttttt accttcagat 60
ctgtctgcta cccgattgtc caaagccaag gagattgggg ctgatttagt cctcccattg 120
cgtgaaccga 130
<210> 37
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 37
gaagcgagga tcaactgggg ctgatttagt cctccagatc tccaaggaga gccctcagga 60
aatcgccagg aaagtagaag gtcagctggg gtgcaagccg gaagtcacca tcgagcattg 120
cgtgaaccga 130
<210> 38
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 38
gaagcgagga tcaactgccg gaagtcacca tcgagtgcac gggggcagag gcctccatcc 60
aggcgggcat ctacgtgagt gggctgaggg cagctttggg gaatcagcat aggggcattg 120
cgtgaaccga 130
<210> 39
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 39
gaagcgagga tcaacttggg gaatcagcat aggggagtga aggaggcaga agtagggagt 60
caaacttctt taccagcttg ctgcgtgagc cccaagccaa acttattcat cttttcattg 120
cgtgaaccga 130
<210> 40
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 40
gaagcgagga tcaacttatg ttattgtaag tgggaagtca gtatttctta tcttctgagc 60
tttcttgttt ttacctcctt tacaggccac tcgctctggt gggaacctcg tgcttcattg 120
cgtgaaccga 130
<210> 41
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 41
gaagcgagga tcaactcagc tcagcttttc tccccgtcac agtgccaacg gctttcagtc 60
cagtgcttgc tacagagagt gctgtgccgc aaagcatggg cccagcccag agagtcattg 120
cgtgaaccga 130
<210> 42
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 42
gaagcgagga tcaacttggg cccagcccag agagtgctct ttgtggttgt tctgtcaggg 60
tagtcaggac tctgcggccc agctgcttct gtgcacagga aataacttca ttttacattg 120
cgtgaaccga 130
<210> 43
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 43
gaagcgagga tcaactattt tgcctgacag agaggaggtc tctgaaatac ttctacgaaa 60
catagtttga gaaccactat tttagctgat accaaatggc catagagctt tactacattg 120
cgtgaaccga 130
<210> 44
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 44
gaagcgagga tcaacttggc catagagctt tactattctg tctggttcta ctatgttata 60
aagactaagt agtttacttt tactttttct ttctttcttt cctttatttt tttttcattg 120
cgtgaaccga 130
<210> 45
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 45
gaagcgagga tcaactccca agggaaggag catgtgtttg ttccaggcaa acttatctac 60
ctgaccacag ctgaatgctt caataatggg ctcctgactc agaagaacca gtccacattg 120
cgtgaaccga 130
<210> 46
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 46
gaagcgagga tcaactactc agaagaacca gtccacaggc tggatagaac cagtcagata 60
gtgtatgggg gccggtttca aattgctaag agagcccatt agcagttagg agccacattg 120
cgtgaaccga 130
<210> 47
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 47
gaagcgagga tcaacttctg aagtaccaga tgccctcccc ccataacctg ccagcgtctt 60
ccacctctag gacttgcctg gcagcttgga agtttgcttc ttggaagcat cctctcattg 120
cgtgaaccga 130
<210> 48
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 48
gaagcgagga tcaactcttc ttggaagcat cctctaccct cccccattcg cagctaacct 60
aaaagtctgc aaccgctccc accgcccgtt caagctggtc acctgagtag gtggacattg 120
cgtgaaccga 130
<210> 49
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 49
gaagcgagga tcaactaggc ctacagggac cgtacagccg gtgattgggc tcccaaccac 60
ctactgaaaa tttaaaggag ctttctgctt tcagatccct atcacgtcct cgactcattg 120
cgtgaaccga 130
<210> 50
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 50
gaagcgagga tcaactccct atcacgtcct cgactttcct gcaagaggca gattattaca 60
gtcccctggg agccactcag ggggagtctc agggcacaga ggttatttga ttcaacattg 120
cgtgaaccga 130
<210> 51
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 51
gaagcgagga tcaactcaat aaccaagaga caatggccag agtcatgagc cctggagata 60
atcagagtgt gacggtgatg gttgtccctg aaagctttgt ccacggagct gctgacattg 120
cgtgaaccga 130
<210> 52
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 52
gaagcgagga tcaactttgt ccacggagct gctgagtcaa tgttgtggat gcccagaaat 60
attaacactt ttttacattt atccattatc tcatttgaaa ctgcaaaagt actgccattg 120
cgtgaaccga 130
<210> 53
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 53
gaagcgagga tcaactcaca gtaaaaaagc aaataataat catcgctcat caagggctat 60
tacttccagc aatgacggtt gaggtcattt ggatcaattc tctgtctgaa catctcattg 120
cgtgaaccga 130
<210> 54
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 54
gaagcgagga tcaactattc tctgtctgaa catctacaga agaaaggcaa atatgaaaaa 60
aaaaaatcct tatgaaacca ttgcagaatt aacaagacag tgaagaatta ccaggcattg 120
cgtgaaccga 130
<210> 55
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 55
gaagcgagga tcaactactg atacccagag tccatgaatc acttcctgaa gccgcacagc 60
tatgtatagc gtaaagatcc aaaccctggt cctttgaccc ttaaggctga gacttcattg 120
cgtgaaccga 130
<210> 56
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 56
gaagcgagga tcaactaccc ttaaggctga gactttccat tatactcaag gcttttaaaa 60
aatggcttgt ctcaaagatt taaacagaca agtcataaaa taagctatga atggtcattg 120
cgtgaaccga 130
<210> 57
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 57
gaagcgagga tcaacttgtt ttgtgatcag agataacttg aaagtctttt tcatcatctg 60
aaagtgacca ggtaagccat gggagctgcc cttgtttcta tccagggagt aaacacattg 120
cgtgaaccga 130
<210> 58
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 58
gaagcgagga tcaacttcta tccagggagt aaacaggagc taacctcatg gaatgtagga 60
gagagattat ttttgttttg ctgattgttt gtttctaggt actaggggtt tttctcattg 120
cgtgaaccga 130
<210> 59
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 59
gaagcgagga tcaactatgc cattccagat ggtttctgct actaatttgt atgtcaggct 60
tcaactctgt gtcattggtc cttacacttt ggggttttga tgtggctgtc caggtcattg 120
cgtgaaccga 130
<210> 60
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 60
gaagcgagga tcaactttga tgtggctgtc caggtgacct agagggaaca gaaatatttc 60
tataaccttc aagtccctta taggaaggag gtaccagaaa tgacctattc tactccattg 120
cgtgaaccga 130
<210> 61
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 61
gaagcgagga tcaactattt ttaggccctc tgatctcaaa tggtcaccta tcggacactc 60
atgcaggctt atttgaaggg tcggtggtct cagtaggctt gaagtgggta agaaccattg 120
cgtgaaccga 130
<210> 62
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 62
gaagcgagga tcaactgctt gaagtgggta agaactgact ctacgttcct gaaaaacaac 60
ttaggcaact gttaccatgg tgactcatac atcagagatg tcgtctataa ggaagcattg 120
cgtgaaccga 130
<210> 63
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 63
gaagcgagga tcaacttcac tttggatttc tttgggttca tttcatatgg cgtccttttc 60
cttccccaga tttgaggagg ttttagccat tatttctttg aatacacatt ctgttcattg 120
cgtgaaccga 130
<210> 64
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 64
gaagcgagga tcaacttttg aatacacatt ctgttccttt ctttatcttc tctttctgga 60
actcctcagt gtatacattg atttgcttga tggtgtccca taattctcta aaaatcattg 120
cgtgaaccga 130
<210> 65
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 65
gaagcgagga tcaacttttg tgtaagtcac tgtatgatgt gcgtgcaaca atgaaattgc 60
ccagcgatgc atgtctcaga acatatccct gttgttaacc atgcatgacc gtattcattg 120
cgtgaaccga 130
<210> 66
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 66
gaagcgagga tcaactaacc atgcatgacc gtattccact cttcagattt catgaagtgt 60
atttgtagat aatcccttta tgtgaaatta tctcatgtga gtaaagaggg gttgtcattg 120
cgtgaaccga 130
<210> 67
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 67
gaagcgagga tcaactgtta ggaagttatt tcaagagtac atcatggtga agcattcaaa 60
acttgtgtct agcatacagt tggtgctcaa taaatgttta caaaagaaag gctggcattg 120
cgtgaaccga 130
<210> 68
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 68
gaagcgagga tcaactttta caaaagaaag gctggaattc atctattgtt ctacatttct 60
gactgatatg gcctcatctc aaaaaaaata tatatctgga aagtattttc tgagtcattg 120
cgtgaaccga 130
<210> 69
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 69
gaagcgagga tcaactcaca gagaagctaa ggaaattgca gaggtcacac agccaggaag 60
ggacagccca aggatttgaa cccaatcagc gtgagtccag catttgtgct cttggcattg 120
cgtgaaccga 130
<210> 70
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 70
gaagcgagga tcaactccag catttgtgct cttggcctcc aggctctgat gcttgcttac 60
tggctaaggt tgaaacaatc ttgtcaattt aaaacgtggc tgcactttag actcacattg 120
cgtgaaccga 130
<210> 71
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 71
gaagcgagga tcaacttcat ttaaacaaca aaagttttca catttatggt caatattttt 60
tgtatcctag aaaatccttt cttgtcccag gtttgcaaga gttcctccca tctgtcattg 120
cgtgaaccga 130
<210> 72
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 72
gaagcgagga tcaactaaga gttcctccca tctgtgtttc tgggaatgtt acagttttgg 60
tttttacatt aggtctgtaa ttcacttcaa gtaattttgt aagatgtgag gtagacattg 120
cgtgaaccga 130
<210> 73
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 73
gaagcgagga tcaacttgga tttgatttta acccaaagtt gatgaggttc cttagtcaca 60
gaaattgcat caggaaaggg aatatgatac aagtcaggcc aacaagagcc aacaacattg 120
cgtgaaccga 130
<210> 74
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 74
gaagcgagga tcaactggcc aacaagagcc aacaagactc aattttagga gttttatctc 60
aactactgag caagaaaaaa aaaaaacttt aaatttggta tagtcaagat gtcatcattg 120
cgtgaaccga 130
<210> 75
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 75
gaagcgagga tcaactgtgt ctcctctcca catgtgcaat gaccagcttt gagtttcaga 60
ttccctggct cccaacactc ggaagcaaaa tataaccatg ggcaggaagg cttgacattg 120
cgtgaaccga 130
<210> 76
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 76
gaagcgagga tcaactcatg ggcaggaagg cttgagagaa gacatgcatt agaaatcagt 60
tgcctcagga ttccatctcc agtgttgtta cacagagcct gggtctctgg ctaaacattg 120
cgtgaaccga 130
<210> 77
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 77
gaagcgagga tcaactaaac taaatgtggt aggtgattgt tcatttcaga taattctgtt 60
aaccctgtga atgaagggcg gttatattgc tagattactc agtcttttgt ttatacattg 120
cgtgaaccga 130
<210> 78
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 78
gaagcgagga tcaactactc agtcttttgt ttatatttga agtcaggcat ggaattgaat 60
tctataaaat tgactttttt tgaaatgatt ttatttgtaa ctaaaagaaa ttacacattg 120
cgtgaaccga 130
<210> 79
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 79
gaagcgagga tcaacttcag gaggatcagt ccagtgggaa tttaccgagc catgccagaa 60
atggcgccat cttctacagc tacattaaca agaaaagaag actcgcctca tttcccattg 120
cgtgaaccga 130
<210> 80
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 80
gaagcgagga tcaactgaag actcgcctca tttccttttc cctagtcttt ctttctccgt 60
ttttttttct cttggagttg tatctccctc cctatggaag tctgcagcct ttttccattg 120
cgtgaaccga 130
<210> 81
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 81
gaagcgagga tcaactacag gcaaggaacc cgcctttaca gaagcacgag tcctttctca 60
ttctttatat gggtctggag gtgtagtagg tgtggaccag acctgtaccg tgggacattg 120
cgtgaaccga 130
<210> 82
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 82
gaagcgagga tcaactactc tacaaaggtc aatattttgc cccttcctga aaaatttatt 60
gtattattag ggatgttata agctacaacc tgggcaactt agaacgacag aaattcattg 120
cgtgaaccga 130
<210> 83
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 83
gaagcgagga tcaactactt agaacgacag aaatttaatg cctcacagtt ctggaggctg 60
aaggttcaag atcaaggtgt cagcaaggtt ggttccttct gaggccatga gggggcattg 120
cgtgaaccga 130
<210> 84
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 84
gaagcgagga tcaactgtta gcatggtgag aagttgtaac actttactcc gtgtacagtt 60
agggatcctc tcctttccgc tccattatga ctaggaataa gaaagatggc atagacattg 120
cgtgaaccga 130
<210> 85
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 85
gaagcgagga tcaactataa gaaagatggc atagaagcct gctgtgtttt ttgtactcag 60
gcttgtgagt ctagtttcta catataaaag tcactcacag gaaaaaatag aatctcattg 120
cgtgaaccga 130
<210> 86
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 86
gaagcgagga tcaacttgtc ataagacatg tgtaagtagc aaagcactgt ctggttttga 60
aatctcaaaa ccatgagtat tgaaatgttg tatttgagtg tttaatgaga agtggcattg 120
cgtgaaccga 130
<210> 87
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 87
gaagcgagga tcaactctca tgtgttttca tgtgttgtca tagccccatg tttggaaatg 60
acaatatgtg acacaagagt gtcaaaatga agacaccaga ggatgacaac agtcacattg 120
cgtgaaccga 130
<210> 88
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 88
gaagcgagga tcaactcaga ggatgacaac agtcaatcaa agcccagtag aatctaggaa 60
ctggaagcaa ggaaggtgag aatttgtcag tgtggcttca tcactaccta ttggtcattg 120
cgtgaaccga 130
<210> 89
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 89
gaagcgagga tcaactcaga ggatgacaac agtcaatcaa agcccagtag aatctaggaa 60
ctggaagcaa ggaaggtgag aatttgtcag tgtggcttca tcactaccta ttggtcattg 120
cgtgaaccga 130
<210> 90
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 90
gaagcgagga tcaactttca tcactaccta ttggtggaag acacatacct gataactact 60
ttgctagttc ttagcacatc agggccattt tctggaggca taatgtaatc tacagcattg 120
cgtgaaccga 130
<210> 91
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 91
gaagcgagga tcaactggca taatgtaatc tacagaaaca gctctcaagc ctgacctttg 60
ctctacagat agcctcagta ttcacctctg attatgtgtc cagattgcag tttcacattg 120
cgtgaaccga 130
<210> 92
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 92
gaagcgagga tcaacttgtc cagattgcag tttcattcct gttgatacgc ctggcttggg 60
tggggacctt ccttccagat cctctaagtt ctctttcata tgcatctcat tccagcattg 120
cgtgaaccga 130
<210> 93
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 93
gaagcgagga tcaactaccc tcactttatg gaaagcatcc agtggggaga ctcatgaaca 60
tcagtcattc tgaggccaca tccttctact aaactaatca ttccgcagac ttggacattg 120
cgtgaaccga 130
<210> 94
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 94
gaagcgagga tcaactatca ttccgcagac ttggatgcac cccgcttcca catggacttt 60
accatccaca acttaccagc aagaatttgg aagattccag tgatgtaaaa cctgccattg 120
cgtgaaccga 130
<210> 95
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 95
gaagcgagga tcaactccag tgatgtaaaa cctgcccccc ttggtgaggg tgaagagttg 60
gcagaagaac aggaacagag acagaatgct gaagatgatc gacaggatca tggtgcattg 120
cgtgaaccga 130
<210> 96
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 96
gaagcgagga tcaactgatc gacaggatca tggtggcctg gacagactgc agccattctg 60
ggggaaagag acacttggtt aggagagctg gccatggccg ggggagggct ctgcccattg 120
cgtgaaccga 130
<210> 97
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 97
gaagcgagga tcaactgccg ggggagggct ctgcctcgag gtgcagggcc tgaagggcca 60
tgactgctga cagcacagtc ctcacaggca gcagaagcag aagtcctttt tccttcattg 120
cgtgaaccga 130
<210> 98
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 98
gaagcgagga tcaacttcat aatattgttt gcttaaaata aaaaaattat ctagcacttt 60
ggtagatgca gctggaatta caaagaaatc tgagactcaa atctgaaact aaaaccattg 120
cgtgaaccga 130
<210> 99
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 99
gaagcgagga tcaacttcaa atctgaaact aaaacaaata aagggattca ctttggctgg 60
gaaatgtcct tccatgtgca tgttcatgag gagtatggct ggtggagaca atatccattg 120
cgtgaaccga 130
<210> 100
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 100
gaagcgagga tcaactggct ggtggagaca atatcttaga tttccttctc caatcctgag 60
atactgtctt cacccttaac cccgcgcccc tgtcattttc agcagaaact actaccattg 120
cgtgaaccga 130
<210> 101
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 101
gaagcgagga tcaacttttc agcagaaact actacgagac ctcacataca cagaaaactt 60
aaaatcttta tatgttaaat ctatataaac agaaagtgtc taatatttaa taatacattg 120
cgtgaaccga 130
<210> 102
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 102
gaagcgagga tcaacttgtc taatatttaa taatataacc taaatacttc ttgaaacatc 60
tttcaacatt aacttgaatt ttagtcccaa ccccattaca attgagctct ttgcccattg 120
cgtgaaccga 130
<210> 103
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 103
gaagcgagga tcaactattc gtagagaaag agccaaagtt gtgtcaaatt gatgtcagtg 60
ggttccccca ggaactactg ttgcaacgaa gaagagtttg gtcaagaagc ctttccattg 120
cgtgaaccga 130
<210> 104
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 104
gaagcgagga tcaacttttg gtcaagaagc ctttcagttg aggatatttt catgtattat 60
aatacttgta ttacttaaat aattaaaacc aatttaaaag attacaaagg taaaacattg 120
cgtgaaccga 130
<210> 105
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 105
gaagcgagga tcaactaaag attacaaagg taaaaacaga tgcagcactc tattgactta 60
ctaagagtca ggattccaaa actcaaaggg ctagtggaat tctgggcagg actggcattg 120
cgtgaaccga 130
<210> 106
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 106
gaagcgagga tcaactgaat tctgggcagg actgggcttc cacatgccca ggaaaacaac 60
aagggatttt cctttcctcc agtgccattg atcaaaagct acacaaggac agcgacattg 120
cgtgaaccga 130
<210> 107
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 107
gaagcgagga tcaactagct acacaaggac agcgagagga aaaaaaacag agcccagccc 60
aggagaaagc aaactccatt ccagctccaa aaggctggtg caagattcaa gagctcattg 120
cgtgaaccga 130
<210> 108
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 108
gaagcgagga tcaactaaaa aaaaaaaaag gccgggcgcc gtggctcaca cctgtaatcc 60
cagcactttg ggaggccaag gcgggtggat cacgaggtca ggagattgag accatcattg 120
cgtgaaccga 130
<210> 109
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 109
gaagcgagga tcaactgtca ggagattgag accatcctgg ctaacacggt gaaacctcgt 60
ctctactaaa aatacaaaaa attagctggg cgtggtggcg ggcacctgta gtccccattg 120
cgtgaaccga 130
<210> 110
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 110
gaagcgagga tcaactggcg ggcacctgta gtcccaacta cttgggaggc tgaggcagga 60
gaatggtgtg aacccgggag gtggagcttg cagtgagccg agatggcgcc actgccattg 120
cgtgaaccga 130
<210> 111
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 111
gaagcgagga tcaactgccg agatggcgcc actgcactcc agcctgggcg acagagcgag 60
actgtctcaa aaacaacaac aacaacaaaa ctaaagctaa agctctttgc aggcccattg 120
cgtgaaccga 130
<210> 112
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 112
gaagcgagga tcaactctaa agctctttgc aggcctcctg ttctgagagt gtccggtata 60
agagctgctg acagctttct gacatgggcc gtggcagcac ccagacctag ctccccattg 120
cgtgaaccga 130
<210> 113
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 113
gaagcgagga tcaactctta gaatccacaa aaactagaga gggtggaagg agaatggtgt 60
ggcaaagaga gagcggcttc ccttattctc tgctcatcag cctgccaagc caaaccattg 120
cgtgaaccga 130
<210> 114
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 114
gaagcgagga tcaacttcag cctgccaagc caaaccactg accaaacttc agaagggcaa 60
gaagaggccc tgcttatgca gctgggacca gcaccagaga agccactgaa agaagcattg 120
cgtgaaccga 130
<210> 115
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 115
gaagcgagga tcaactgaga agccactgaa agaaggaagg aaagaaaaac tgcaatcgtc 60
aactgtttga agagtcatcg tgtagaggag agacgggggt caccctgcag tcattcattg 120
cgtgaaccga 130
<210> 116
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 116
gaagcgagga tcaactgggt caccctgcag tcattgtcca ggaggcactg tggagattta 60
aattctttaa ggacgtgcca agcaacttga taaaacccaa gtccagcaca tcaggcattg 120
cgtgaaccga 130
<210> 117
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 117
gaagcgagga tcaactccaa gtccagcaca tcaggcatga atgaaggcaa ctagaagtga 60
atggacaaaa gaggggcagt gttgggttta acaaggtaat ttcttttttt gagaccattg 120
cgtgaaccga 130
<210> 118
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 118
gaagcgagga tcaacttata gattccaggt catacagttc agacacaggt tcaaacaaac 60
atttcatact aattcctggg tattggcatc agagaaaccc taagggttga aatcccattg 120
cgtgaaccga 130
<210> 119
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 119
gaagcgagga tcaactaccc taagggttga aatcctggca tgttgacaac acattccgtc 60
ctctgtacct cccttgtacc accagggtct cagcatctca taagagcctg taaaacattg 120
cgtgaaccga 130
<210> 120
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 120
gaagcgagga tcaactctca taagagcctg taaaatccag agccattcct tgggtagaga 60
ttatgggttg gctgagggag ccacgaagat tttaaaatgt aaaacatgaa cccatcattg 120
cgtgaaccga 130
<210> 121
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 121
gaagcgagga tcaactatgt aaaacatgaa cccataaatg cagaaggtct cccgtgtttt 60
ctaaaatctg gccaaaagct gagccacaga caaggaaatt gtctggtgtc cttgccattg 120
cgtgaaccga 130
<210> 122
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 122
gaagcgagga tcaactaatt gtctggtgtc cttgccaaaa tgacagtgtc accaaattga 60
taaacaagtc tgggatcgtt ttgaagacag cgatgatagg agaccaccaa gggctcattg 120
cgtgaaccga 130
<210> 123
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 123
gaagcgagga tcaactgtta ggaggctgcc ctggcgcctc tgacaaacga ggaagcagcg 60
tgaccacagg tgggcaacag gcattggaac aatggggtgg cctgagggac ccgtgcattg 120
cgtgaaccga 130
<210> 124
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 124
gaagcgagga tcaactgtgg cctgagggac ccgtgacaat gctgagaaga gaaagaagga 60
aacgcgtcaa gtcaaggcag actctagatg tttggcttgc tggctggtga agcagcattg 120
cgtgaaccga 130
<210> 125
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 125
gaagcgagga tcaactttgc tggctggtga agcagcattc actaaaatac tagaaagtga 60
cagaggggtt gaattggcag aggaagataa ggactgtctc tggcctagtg acttgcattg 120
cgtgaaccga 130
<210> 126
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 126
gaagcgagga tcaacttctc tggcctagtg acttgggggc ctctcttaga tatcccaagg 60
gaagtggcca ggagacagct ggataaacag gtctgggatt cagaagagag gtctgcattg 120
cgtgaaccga 130
<210> 127
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 127
gaagcgagga tcaactgatt cagaagagag gtctggactc attaaataat aataagatgg 60
ccaggcatgg tggctcacgc ttgtaatctc agcactttgg gaggctgagg caggtcattg 120
cgtgaaccga 130
<210> 128
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 128
gaagcgagga tcaactagag aggtatttgc tgccaggttt ctatgatttg aaaagccatg 60
cttcccccaa ggccgaatga ctatatttct cagcctctct tgtaaatagg tgtagcattg 120
cgtgaaccga 130
<210> 129
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 129
gaagcgagga tcaactctct tgtaaatagg tgtaggtgtg accatgtcac atttcattaa 60
aagaaaagga gattcattct tccccacctc ctttctccac cctactgctt gaatccattg 120
cgtgaaccga 130
<210> 130
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 130
gaagcgagga tcaacttcgg gcagcggctg tttctgttgg atgcactggg tcacccacca 60
gaaaagggct tttggacatt tggggtttct acccacactt tggttttcta aatgacattg 120
cgtgaaccga 130
<210> 131
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 131
gaagcgagga tcaactactt tggttttcta aatgaggtgg actgggaggg aggtatcttc 60
tttcagatga aagggaaggg gcgagatgga gttatcttat ttctgggtaa aacaacattg 120
cgtgaaccga 130
<210> 132
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 132
gaagcgagga tcaactagct gagtggacca gctactcccc gatttcactg actaagagga 60
gataccagat tccatccttg aacaagaggg atagaaagag gcagtataga gaagtcattg 120
cgtgaaccga 130
<210> 133
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 133
gaagcgagga tcaactagag gcagtataga gaagtgcagc gactaactac acagcttaat 60
gtgtatgact ttcatcttag tagaatctca gaagtgaatg actctaagct taacccattg 120
cgtgaaccga 130
<210> 134
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 134
gaagcgagga tcaactgaga aaaaaaaaaa gaaaccccag aatatgttac agttgttttt 60
attccaactg aaaattgagt aagggtaact cttcacttta acgtacctag aaatgcattg 120
cgtgaaccga 130
<210> 135
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 135
gaagcgagga tcaactttta acgtacctag aaatgatatc tccttgcata attagtcatt 60
taacagagcc agcaaccatc caggagcttg gcttggtgtc ataagacatg tgtaacattg 120
cgtgaaccga 130
<210> 136
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 136
gaagcgagga tcaactttca tcactaccta ttggtggaag acacatacct gataactact 60
ttgctagttc ttagcacatc agggccattt tctggaggca taatgtaatc tacagcattg 120
cgtgaaccga 130
<210> 137
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 137
gaagcgagga tcaactggca taatgtaatc tacagaaaca gctctcaagc ctgacctttg 60
ctctacagat agcctcagta ttcacctctg attatgtgtc cagattgcag tttcacattg 120
cgtgaaccga 130
<210> 138
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 138
gaagcgagga tcaactggct ggtggagaca atatcttaga tttccttctc caatcctgag 60
atactgtctt cacccttaac cccgcgcccc tgtcattttc agcagaaact actaccattg 120
cgtgaaccga 130
<210> 139
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 139
gaagcgagga tcaacttttc agcagaaact actacgagac ctcacataca cagaaaactt 60
aaaatcttta tatgttaaat ctatataaac agaaagtgtc taatatttaa taatacattg 120
cgtgaaccga 130
<210> 140
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 140
gaagcgagga tcaactaaag attacaaagg taaaaacaga tgcagcactc tattgactta 60
ctaagagtca ggattccaaa actcaaaggg ctagtggaat tctgggcagg actggcattg 120
cgtgaaccga 130
<210> 141
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 141
gaagcgagga tcaactgaat tctgggcagg actgggcttc cacatgccca ggaaaacaac 60
aagggatttt cctttcctcc agtgccattg atcaaaagct acacaaggac agcgacattg 120
cgtgaaccga 130
<210> 142
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 142
gaagcgagga tcaactggcg ggcacctgta gtcccaacta cttgggaggc tgaggcagga 60
gaatggtgtg aacccgggag gtggagcttg cagtgagccg agatggcgcc actgccattg 120
cgtgaaccga 130
<210> 143
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 143
gaagcgagga tcaactgccg agatggcgcc actgcactcc agcctgggcg acagagcgag 60
actgtctcaa aaacaacaac aacaacaaaa ctaaagctaa agctctttgc aggcccattg 120
cgtgaaccga 130
<210> 144
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 144
gaagcgagga tcaactgaga agccactgaa agaaggaagg aaagaaaaac tgcaatcgtc 60
aactgtttga agagtcatcg tgtagaggag agacgggggt caccctgcag tcattcattg 120
cgtgaaccga 130
<210> 145
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 145
gaagcgagga tcaactgggt caccctgcag tcattgtcca ggaggcactg tggagattta 60
aattctttaa ggacgtgcca agcaacttga taaaacccaa gtccagcaca tcaggcattg 120
cgtgaaccga 130
<210> 146
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 146
gaagcgagga tcaactctca taagagcctg taaaatccag agccattcct tgggtagaga 60
ttatgggttg gctgagggag ccacgaagat tttaaaatgt aaaacatgaa cccatcattg 120
cgtgaaccga 130
<210> 147
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 147
gaagcgagga tcaactatgt aaaacatgaa cccataaatg cagaaggtct cccgtgtttt 60
ctaaaatctg gccaaaagct gagccacaga caaggaaatt gtctggtgtc cttgccattg 120
cgtgaaccga 130
<210> 148
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 148
gaagcgagga tcaactttgc tggctggtga agcagcattc actaaaatac tagaaagtga 60
cagaggggtt gaattggcag aggaagataa ggactgtctc tggcctagtg acttgcattg 120
cgtgaaccga 130
<210> 149
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 149
gaagcgagga tcaacttctc tggcctagtg acttgggggc ctctcttaga tatcccaagg 60
gaagtggcca ggagacagct ggataaacag gtctgggatt cagaagagag gtctgcattg 120
cgtgaaccga 130
<210> 150
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 150
gaagcgagga tcaactccac cctactgctt gaatcatgga tgagatggct ggagctctag 60
cagccttttg tttcctgaag atgacagcca aacccaagag atggcaaaga aatgacattg 120
cgtgaaccga 130
<210> 151
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 151
gaagcgagga tcaactgttg actaagtcaa taatcagaat cagcaggttt gcagtcagat 60
tggcagggat aagcagccta gctcaggaga agtgagtata aaagccccag gctggcattg 120
cgtgaaccga 130
<210> 152
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 152
gaagcgagga tcaacttata aaagccccag gctgggagca gccatcacag aagtccactc 60
attcttggca ggatggcttc tcatcgtctg ctcctcctct gccttgctgg actggcattg 120
cgtgaaccga 130
<210> 153
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 153
gaagcgagga tcaactctct gccttgctgg actggtattt gtgtctgagg ctggccctac 60
ggtgagtgtt tctgtgacat cccattccta catttaagat tcacgctaaa tgaagcattg 120
cgtgaaccga 130
<210> 154
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 154
gaagcgagga tcaactagat tcacgctaaa tgaagtagaa gtgactcctt ccagctttgc 60
caaccagctt ttattactag ggcaagggta cccagcatct atttttaata taattcattg 120
cgtgaaccga 130
<210> 155
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 155
gaagcgagga tcaactgctc attaattgtc gacacttacg ttcctgataa tgggatcagt 60
gtgtaattct tgtttcgctc cagatttcta ataccacaaa gaataaatcc tttcacattg 120
cgtgaaccga 130
<210> 156
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 156
gaagcgagga tcaactcaaa gaataaatcc tttcactctg atcaattttg ttaacttctc 60
acgtgtcttc tctacaccca gggcaccggt gaatccaagt gtcctctgat ggtcacattg 120
cgtgaaccga 130
<210> 157
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 157
gaagcgagga tcaactaagt gtcctctgat ggtcaaagtt ctagatgctg tccgaggcag 60
tcctgccatc aatgtggccg tgcatgtgtt cagaaaggct gctgatgaca cctggcattg 120
cgtgaaccga 130
<210> 158
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 158
gaagcgagga tcaactggct gctgatgaca cctgggagcc atttgcctct gggtaagttg 60
ccaaagaacc ctcccacagg acttggtttt atcttcccgt ttgcccctca cttggcattg 120
cgtgaaccga 130
<210> 159
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 159
gaagcgagga tcaactccgt ttgcccctca cttggtagag agaggctcac atcatctgct 60
aaagaattta caagtagatt gaaaaacgta ggcagaggtc aagtatgccc tctgacattg 120
cgtgaaccga 130
<210> 160
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 160
gaagcgagga tcaactccta cttctgactt agttgagggg aaatgttcat aacatgttta 60
taacatgttt atgtgtgtta gttggtgggg gtgtattact ttgccatgcc atttgcattg 120
cgtgaaccga 130
<210> 161
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 161
gaagcgagga tcaacttact ttgccatgcc atttgtttcc tccatgcgta acttaatcca 60
gactttcaca ccttatagga aaaccagtga gtctggagag ctgcatgggc tcacacattg 120
cgtgaaccga 130
<210> 162
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 162
gaagcgagga tcaactagag ctgcatgggc tcacaactga ggaggaattt gtagaaggga 60
tatacaaagt ggaaatagac accaaatctt actggaaggc acttggcatc tcccccattg 120
cgtgaaccga 130
<210> 163
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 163
gaagcgagga tcaactaggc acttggcatc tccccattcc atgagcatgc agaggtgagt 60
atacagacct tcgagggttg ttttggtttt ggtttttgct tttggcattc caggacattg 120
cgtgaaccga 130
<210> 164
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 164
gaagcgagga tcaacttgct tttggcattc caggaaatgc acagttttac tcagtgtacc 60
acagaaatgt cctaaggaag gtgatgaatg accaaaggtt ccctttccta ttatacattg 120
cgtgaaccga 130
<210> 165
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 165
gaagcgagga tcaactcata tttgagcttt ttccttctgt tcaaactgtt ccaaaatata 60
aaagaataaa attaattaag ttggcactgg acttccggtg gtcagtcatg tgtgtcattg 120
cgtgaaccga 130
<210> 166
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 166
gaagcgagga tcaactggtg gtcagtcatg tgtgtcatct gtcacgtttt tcgggctctg 60
gtggaaatgg atctgtctgt cttctctcat aggtggtatt cacagccaac gactccattg 120
cgtgaaccga 130
<210> 167
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 167
gaagcgagga tcaacttatt cacagccaac gactccggcc cccgccgcta caccattgcc 60
gccctgctga gcccctactc ctattccacc acggctgtcg tcaccaatcc caaggcattg 120
cgtgaaccga 130
<210> 168
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 168
gaagcgagga tcaactgtcg tcaccaatcc caaggaatga gggacttctc ctccagtgga 60
cctgaaggac gagggatggg atttcatgta accaagagta ttccattttt actaacattg 120
cgtgaaccga 130
<210> 169
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 169
gaagcgagga tcaactagta ttccattttt actaaagcag tgttttcacc tcatatgcta 60
tgttagaagt ccaggcagag acaataaaac attcctgtga aaggcacttt tcattcattg 120
cgtgaaccga 130
<210> 170
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 170
gaagcgagga tcaacttgcc gcagtgcctc ccgccccgtc ccggcctcgc gcacctgctc 60
aggccatgat gagcttcggc ggcgcggacg cgctgctggg cgccccgttc gcgcccattg 120
cgtgaaccga 130
<210> 171
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 171
gaagcgagga tcaacttggg cgccccgttc gcgccgctgc atggcggcgg cagcctccac 60
tacgcgctag cccgaaaggg tggcgcaggc gggacgcgct ccgccgctgg ctcctcattg 120
cgtgaaccga 130
<210> 172
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 172
gaagcgagga tcaactcgct ccgccgctgg ctcctccagc ggcttccact cgtggacacg 60
gacgtccgtg agctccgtgt ccgcctcgcc cagccgcttc cgtggcgcag gcgcccattg 120
cgtgaaccga 130
<210> 173
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 173
gaagcgagga tcaactcttc cgtggcgcag gcgccgcctc aagcaccgac tcgctggaca 60
cgctgagcaa cgggccggag ggctgcatgg tggcggtggc cacctcacgc agtgacattg 120
cgtgaaccga 130
<210> 174
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 174
gaagcgagga tcaacttggc cacctcacgc agtgagaagg agcagctgca ggcgctgaac 60
gaccgcttcg ccgggtacat cgacaaggtg cggcagctgg aggcgcacaa ccgcacattg 120
cgtgaaccga 130
<210> 175
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 175
gaagcgagga tcaactctgg aggcgcacaa ccgcagcctg gagggcgagg ctgcggcgct 60
gcggcagcag caggcgggcc gctccgctat gggcgagctg tacgagcgcg aggtccattg 120
cgtgaaccga 130
<210> 176
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 176
gaagcgagga tcaactgctg tacgagcgcg aggtccgcga gatgcgcggc gcggtgctgc 60
gcctgggcgc ggcgcgcggt cagctacgcc tggagcagga gcacctgctc gaggacattg 120
cgtgaaccga 130
<210> 177
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 177
gaagcgagga tcaactagga gcacctgctc gaggacatcg cgcacgtgcg ccagcgccta 60
gacgacgagg cccggcagcg agaggaggcc gaggcggcgg cccgcgcgct ggcgccattg 120
cgtgaaccga 130
<210> 178
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 178
gaagcgagga tcaactgcgg cccgcgcgct ggcgcgcttc gcgcaggagg ccgaggcggc 60
gcgcgtggac ctgcagaaga aggcgcaggc gctgcaggag gagtgcggct acctgcattg 120
cgtgaaccga 130
<210> 179
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 179
gaagcgagga tcaactggag gagtgcggct acctgcggcg ccaccaccag gaagaggtgg 60
gcgagctgct cggccagatc cagggctccg gcgccgcgca ggcgcagatg caggccattg 120
cgtgaaccga 130
<210> 180
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 180
gaagcgagga tcaactcgca ggcgcagatg caggccgaga cgcgcgacgc cctgaagtgc 60
gacgtgacgt cggcgctgcg cgagattcgc gcgcagcttg aaggccacgc ggtgccattg 120
cgtgaaccga 130
<210> 181
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 181
gaagcgagga tcaactcttg aaggccacgc ggtgcagagc acgctgcagt ccgaggagtg 60
gttccgaggt acgcaggcgc gcgggtgggg ggaggggcgc ccctgctgac cccgccattg 120
cgtgaaccga 130
<210> 182
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 182
gaagcgagga tcaactgcgc ccctgctgac cccgcagcga acttttgggc tgcgtgaccc 60
aagggggcgc tgccggactg cgcgtggagt ggcgcgctgc tcaccttccc tctgccattg 120
cgtgaaccga 130
<210> 183
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 183
gaagcgagga tcaactgctg agagtgcacc actgccctcc agcctgggcg agagcgagaa 60
tccgtctcaa aaaaaaaaaa aaagaaaaag aacaaagaaa aaaatctggg cattacattg 120
cgtgaaccga 130
<210> 184
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 184
gaagcgagga tcaactgaaa aaaatctggg cattagaacc cagtccaggt gtgtctaacc 60
ctgtgccctg ctaccttctc ccccagtgag gctggaccga ctgtcggagg cagcccattg 120
cgtgaaccga 130
<210> 185
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 185
gaagcgagga tcaactccga ctgtcggagg cagccaaggt gaacacagac gctatgcgct 60
cagcgcagga ggagataact gagtaccggc gtcagctgca ggccaggacc acagacattg 120
cgtgaaccga 130
<210> 186
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 186
gaagcgagga tcaacttgca ggccaggacc acagagctgg aggcactgaa aagcaccaag 60
gactcactgg agaggcagcg ctctgagctg gaggaccgtc atcaggccga cattgcattg 120
cgtgaaccga 130
<210> 187
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 187
gaagcgagga tcaactcgtc atcaggccga cattgcctcc taccaggtgg gcaggggcaa 60
ggcagacagc cagactgcct tacctgattg ggtagccctg gacaagttac tgtcccattg 120
cgtgaaccga 130
<210> 188
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 188
gaagcgagga tcaactcctg gacaagttac tgtccctcac tgagtggggt tgtagtggtt 60
aagattgtgg cccttggagt cagacatacc agcactttcc agctgtgtga ccctacattg 120
cgtgaaccga 130
<210> 189
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 189
gaagcgagga tcaactgacc ctacagctct ggcagggttg gggttggccc cagtgagcct 60
ggctggatgg gcctgctctg gggtcgcaca gcccgccgca gcttctctgg tactgcattg 120
cgtgaaccga 130
<210> 190
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 190
gaagcgagga tcaactcgca gcttctctgg tactgagggc cagacactgg ctggcatgtg 60
atgtgtgtca cctctccttc ccaggaagcc attcagcagc tggacgctga gctgacattg 120
cgtgaaccga 130
<210> 191
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 191
gaagcgagga tcaactcagc tggacgctga gctgaggaac accaagtggg agatggccgc 60
ccagctgcga gaataccagg acctgctcaa tgtcaagatg gctctggata tagagcattg 120
cgtgaaccga 130
<210> 192
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 192
gaagcgagga tcaactgatg gctctggata tagagatagc cgcttacagg tgagacgcac 60
aggggctgtc acatggtgaa gaaagcttgt gttcctgcga gacgctaagc cttggcattg 120
cgtgaaccga 130
<210> 193
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 193
gaagcgagga tcaactgcga gacgctaagc cttgggtttc aagaccccgt ttaggcagcc 60
tacctgtctt agggacaaaa ttctttttaa attgcggcaa aatagacaaa acatacattg 120
cgtgaaccga 130
<210> 194
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 194
gaagcgagga tcaactcatt cctgttccac caaaacccat atcattcgat gtcagtgacc 60
ttcaggatag tctaggaaga acccaaatag tgaatttgcc ctgagtttat actaacattg 120
cgtgaaccga 130
<210> 195
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 195
gaagcgagga tcaacttgcc ctgagtttat actaatgtgt tccgtgatcc atcctgcaga 60
aaactcctgg aaggtgaaga gtgtcggatt ggctttggcc caattccttt ctcgccattg 120
cgtgaaccga 130
<210> 196
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 196
gaagcgagga tcaactggcc caattccttt ctcgcttcca gaaggactcc ccaaaattcc 60
ctctgtgtcc actcacataa aggtgaaaag cgaagagaag atcaaagtgg tggagcattg 120
cgtgaaccga 130
<210> 197
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 197
gaagcgagga tcaactgaag atcaaagtgg tggagaagtc tgagaaagaa actgtgattg 60
tggaggaaca gacagaggag acccaagtga ctgaagaagt gactgaagaa gaggacattg 120
cgtgaaccga 130
<210> 198
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 198
gaagcgagga tcaactaagt gactgaagaa gaggagaaag aggccaaaga ggaggagggc 60
aaggaggaag aagggggtga agaagaggag gcagaagggg gagaagaaga aacaacattg 120
cgtgaaccga 130
<210> 199
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 199
gaagcgagga tcaactgggg gagaagaaga aacaaagtct cccccagcag aagaggctgc 60
atccccagag aaggaagcca agtcaccagt aaaggaagag gcaaagtcac cggctcattg 120
cgtgaaccga 130
<210> 200
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 200
gaagcgagga tcaactagag gcaaagtcac cggctgaggc caagtcccca gagaaggagg 60
aagcaaaatc cccagccgaa gtcaagtccc ctgagaaggc caagtctcca gcaaacattg 120
cgtgaaccga 130
<210> 201
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 201
gaagcgagga tcaactaggc caagtctcca gcaaaggaag aggcaaagtc accgcctgag 60
gccaagtccc cagagaagga ggaagcaaaa tctccagctg aggtcaagtc ccccgcattg 120
cgtgaaccga 130
<210> 202
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 202
gaagcgagga tcaactgctg aggtcaagtc ccccgagaag gccaagtccc cagcaaagga 60
agaggcaaag tcaccggctg aggccaagtc tccagagaag gccaagtccc cagtgcattg 120
cgtgaaccga 130
<210> 203
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 203
gaagcgagga tcaactgaag gccaagtccc cagtgaagga agaagcaaag tcaccggctg 60
aggccaagtc cccagtgaag gaagaagcaa aatctccagc tgaggtcaag tcccccattg 120
cgtgaaccga 130
<210> 204
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 204
gaagcgagga tcaactcagc tgaggtcaag tccccggaaa aggccaagtc tccaacgaag 60
gaggaagcaa agtcccctga gaaggccaag tccccagaga aggaagaggc caagtcattg 120
cgtgaaccga 130
<210> 205
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 205
gaagcgagga tcaactgaga aggaagaggc caagtcccct gagaaggcca agtccccagt 60
gaaggcagaa gcaaagtccc ctgagaaggc caagtcccca gtgaaggcag aagcacattg 120
cgtgaaccga 130
<210> 206
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 206
gaagcgagga tcaactccca gtgaaggcag aagcaaagtc ccctgagaag gccaagtccc 60
cagtgaagga agaagcaaag tcccctgaga aggccaagtc cccagtgaag gaagacattg 120
cgtgaaccga 130
<210> 207
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 207
gaagcgagga tcaactagtc cccagtgaag gaagaagcaa agtcccctga gaaggccaag 60
tccccagtga aggaagaagc aaagaccccc gagaaggcca agtccccagt gaaggcattg 120
cgtgaaccga 130
<210> 208
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 208
gaagcgagga tcaactagtc cccagtgaag gaagaagcaa agtcccctga gaaggccaag 60
tccccagtga aggaagaagc aaagaccccc gagaaggcca agtccccagt gaaggcattg 120
cgtgaaccga 130
<210> 209
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 209
gaagcgagga tcaactgtct ccagaagcca agactccagc gaaggaggaa gcaaggtccc 60
ctgcagacaa attccctgaa aaggccaaaa gccctgtcaa ggaggaggtc aagtccattg 120
cgtgaaccga 130
<210> 210
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 210
gaagcgagga tcaacttcaa ggaggaggtc aagtccccag agaaggcgaa atctcccctg 60
aaggaggatg ccaaggcccc tgagaaggag atcccaaaaa aggaagaggt gaagtcattg 120
cgtgaaccga 130
<210> 211
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 211
gaagcgagga tcaactaaaa aggaagaggt gaagtcccca gtgaaggagg aggagaagcc 60
ccaggaggtg aaagtcaaag agcccccaaa gaaggcagag gaagagaaag cccctcattg 120
cgtgaaccga 130
<210> 212
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 212
gaagcgagga tcaactagag gaagagaaag cccctgccac accaaaaaca gaggagaaga 60
aggacagcaa gaaagaggag gcacccaaga aggaggctcc aaagcccaag gtggacattg 120
cgtgaaccga 130
<210> 213
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 213
gaagcgagga tcaactctcc aaagcccaag gtggaggaga agaaggaacc tgctgtcgaa 60
aagcccaaag aatccaaagt tgaagccaag aaggaagagg ctgaagataa gaaaacattg 120
cgtgaaccga 130
<210> 214
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 214
gaagcgagga tcaactgagg ctgaagataa gaaaaaagtc cccaccccag agaaggaggc 60
tcctgccaag gtggaggtga aggaagacgc taaacccaaa gaaaagacag aggtacattg 120
cgtgaaccga 130
<210> 215
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 215
gaagcgagga tcaactcaaa gaaaagacag aggtagccaa gaaggaacca gatgatgcca 60
aggccaagga acccagcaaa ccagcagaga agaaggaggc agcaccggag aaaaacattg 120
cgtgaaccga 130
<210> 216
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 216
gaagcgagga tcaactaggc agcaccggag aaaaaagaca ccaaggagga gaaggccaag 60
aagcctgagg agaaacccaa gacagaggcc aaagccaagg aagatgacaa gaccccattg 120
cgtgaaccga 130
<210> 217
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 217
gaagcgagga tcaactaagg aagatgacaa gaccctctca aaagagccta gcaagcctaa 60
ggcagaaaag gctgaaaaat cctccagcac agaccaaaaa gacagcaagc ctccacattg 120
cgtgaaccga 130
<210> 218
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 218
gaagcgagga tcaactaaaa gacagcaagc ctccagagaa ggccacagaa gacaaggccg 60
ccaaggggaa gtaaggcagg gagaaaggaa catccggaac agccaaagaa actcacattg 120
cgtgaaccga 130
<210> 219
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 219
gaagcgagga tcaactgaac agccaaagaa actcagaaga gtcccggagc tcaaggatca 60
gagtaacaca attttcactt tttctgtctt tatgtaagaa gaaactgctt agatgcattg 120
cgtgaaccga 130
<210> 220
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 220
gaagcgagga tcaactcttt tcctccaaaa acacaaatgt cccgtgtaag tcaaaccaag 60
gtgcgaccac caacccatga atgaagtccc ctccccctgc agctacaggg ttgagcattg 120
cgtgaaccga 130
<210> 221
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 221
gaagcgagga tcaactctgc agctacaggg ttgaggggca ggtgggcagg ggagctgctc 60
tctctcctgc cttcagtccc ccttggtgat gaggtcctca atgtaggcgt ccagccattg 120
cgtgaaccga 130
<210> 222
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 222
gaagcgagga tcaactctca atgtaggcgt ccagctcatc atctgtgttg atgtctatgt 60
cctggaatag agcagggtag ggatcaactg ggggccagaa agtgattggg gggctcattg 120
cgtgaaccga 130
<210> 223
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 223
gaagcgagga tcaactagaa agtgattggg gggctcacct ttccaccctt ctctcagctc 60
ctgggatgga gcccagagtc ctctccctga cctcccaccc cgccctggat gtcagcattg 120
cgtgaaccga 130
<210> 224
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 224
gaagcgagga tcaactcatc tcaagccctc ctgccagggt gctgtgcagc cagggtgaga 60
accatagatt cctgtgagtg gatagattct tgtgagaaca ggaacagggc ccaggcattg 120
cgtgaaccga 130
<210> 225
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 225
gaagcgagga tcaactaaca ggaacagggc ccaggccccc ctccctgaga gaccaggtgg 60
tccagctgca acaggaaggc tgccgggctc acctcctgca ccttttgcag gagtgcattg 120
cgtgaaccga 130
<210> 226
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 226
gaagcgagga tcaacttgca ccttttgcag gagtgccacg atgttggtcc tcagcttctg 60
cagcttctcc tccgtctcgc ggagcttcct ctcggaggtg cgcaggcttt cctcgcattg 120
cgtgaaccga 130
<210> 227
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 227
gaagcgagga tcaactggtg cgcaggcttt cctcggaggc cttggcccgg gagtcagcac 60
ggctctggta ggaattgcac aggttttgga gccctacttc atattgcttg aagtacattg 120
cgtgaaccga 130
<210> 228
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 228
gaagcgagga tcaactcttc atattgcttg aagtactcct tctgttggga gcagagcaag 60
agggagagtg tgtaagttca caggggtccc cagggccctt ctaggctgac acgggcattg 120
cgtgaaccga 130
<210> 229
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 229
gaagcgagga tcaactcctt ctaggctgac acgggtgtct ccagcccgct ttacccaagg 60
atgcctggtc tgaaggaaca tcttagatac aaagggcaaa cagcctcttt acgtccattg 120
cgtgaaccga 130
<210> 230
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 230
gaagcgagga tcaacttttc agcaggagag aggctggctg agatggagca cacagctgag 60
agctagcaac tgcaacaagc gtaacaatca taatcacaac agttacaaca aatgccattg 120
cgtgaaccga 130
<210> 231
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 231
gaagcgagga tcaactcaac agttacaaca aatgcagggg caggggtggg ggaatgaaga 60
gtgtggaatc gaaggtcagg ccagactcac cagaggaaaa gatattagct catctcattg 120
cgtgaaccga 130
<210> 232
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 232
gaagcgagga tcaactaaaa gatattagct catctgaatt catagcactc agctgcttct 60
tggagatggg gaaacttgga ggcaggaagt accgtaaaca attcctaaag aaggccattg 120
cgtgaaccga 130
<210> 233
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 233
gaagcgagga tcaactaaca attcctaaag aaggcaggga cagtaattca gaatggcatg 60
gagcaggcag agagctgctg ctggccctgg ggtgggaaga caaaagacac atgtacattg 120
cgtgaaccga 130
<210> 234
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 234
gaagcgagga tcaactaaga caaaagacac atgtaccgga ggatctggac aagcaggtcg 60
atggtctcgt gattggtgct cagggcagtg gtgtcaggct caatgcggag gcattcattg 120
cgtgaaccga 130
<210> 235
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 235
gaagcgagga tcaactggct caatgcggag gcattcggaa gtggagggct ctgcgacagc 60
tatggcctgc tgggccacag ggcccacctc ctcctccccg ccctcctgtt gtgtacattg 120
cgtgaaccga 130
<210> 236
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 236
gaagcgagga tcaactcccg ccctcctgtt gtgtatcaag gctctgatgt tccggagaag 60
ggggtttcat cagccgcaca tcctcactgc ctttctccac cctgtggaag acacacattg 120
cgtgaaccga 130
<210> 237
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 237
gaagcgagga tcaactccac cctgtggaag acacacagct tatgtcatgt ctgacccggg 60
ctcccaggat gggctgctgg cagggaggcc ggggatacct ccttcgagga accaccattg 120
cgtgaaccga 130
<210> 238
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 238
gaagcgagga tcaactacct ccttcgagga accactgctc ctccctgatt ggggcattac 60
cagcggtctc ttggtgtcgt gtctgtgggc acgtagtcaa acttcacctt ccacccattg 120
cgtgaaccga 130
<210> 239
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 239
gaagcgagga tcaacttcaa acttcacctt ccaccgcacc acatgcttgc ccacctccac 60
ggctgtgaca cggcccgtgt accactccct gttcacacgc acctccacgt gcagccattg 120
cgtgaaccga 130
<210> 240
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 240
gaagcgagga tcaactacgc acctccacgt gcagcccttt atctgacaat gtagacagag 60
gtgcgagtca gaaaactagc gtagagtccc tcacttggcc tgggccacat cgagacattg 120
cgtgaaccga 130
<210> 241
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 241
gaagcgagga tcaactggcc tgggccacat cgagaaaggc agtccccagg gtttggagga 60
caggaacact taggtgcttg cccccactac accagggaca cagagaccag ggacccattg 120
cgtgaaccga 130
<210> 242
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 242
gaagcgagga tcaactgagg ggcggccctc aaacgggtca ttgccattaa tagagacctc 60
aaacaccgcc tgctaaaaat acccgactgg aggagcataa aagcgcagcc gagcccattg 120
cgtgaaccga 130
<210> 243
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 243
gaagcgagga tcaactataa aagcgcagcc gagcccagcg ccccgcactt ttctgagcag 60
acgtccagag cagagtcagc cagcatgacc gagcgccgcg tccccttctc gctcccattg 120
cgtgaaccga 130
<210> 244
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 244
gaagcgagga tcaactcgcg tccccttctc gctcctgcgg ggccccagct gggacccctt 60
ccgcgactgg tacccgcata gccgcctctt cgaccaggcc ttcgggctgc cccggcattg 120
cgtgaaccga 130
<210> 245
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 245
gaagcgagga tcaactggcc ttcgggctgc cccggctgcc ggaggagtgg tcgcagtggt 60
taggcggcag cagctggcca ggctacgtgc gccccctgcc ccccgccgcc atcgacattg 120
cgtgaaccga 130
<210> 246
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 246
gaagcgagga tcaacttgcc ccccgccgcc atcgagagcc ccgcagtggc cgcgcccgcc 60
tacagccgcg cgctcagccg gcaactcagc agcggggtct cggagatccg gcacacattg 120
cgtgaaccga 130
<210> 247
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 247
gaagcgagga tcaactgtct cggagatccg gcacactgcg gaccgctggc gcgtgtccct 60
ggatgtcaac cacttcgccc cggacgagct gacggtcaag accaaggatg gcgtgcattg 120
cgtgaaccga 130
<210> 248
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 248
gaagcgagga tcaactcaag accaaggatg gcgtggtgga gatcaccggt gagcccccct 60
gctcctgcag gggagaggag gaggctagca gggcgggcag ggccgggggc gtgcgcattg 120
cgtgaaccga 130
<210> 249
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 249
gaagcgagga tcaactgcag ggccgggggc gtgcggttga aacgggggtc ccgggggcct 60
ggggagttaa acgttggccc agcaccggga aaaacaggac tcctgattcc cttgccattg 120
cgtgaaccga 130
<210> 250
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 250
gaagcgagga tcaactcacg cccccatccc caaccccctc tgttaatccc taccagcctg 60
cagtcctggc tgcttccaag caggaggtgg ggcctctggc ctagcggggc cgaaacattg 120
cgtgaaccga 130
<210> 251
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 251
gaagcgagga tcaacttggc ctagcggggc cgaaaggcag tcccctcccc cgcagtctga 60
tttccctctt ccccccaaag gcaagcacga ggagcggcag gacgagcatg gctaccattg 120
cgtgaaccga 130
<210> 252
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 252
gaagcgagga tcaactgcag gacgagcatg gctacatctc ccggtgcttc acgcggaaat 60
acacgtgagt cctggcgcca ggtcggggtg ggtgggtggc gtgggggtgg ggtcacattg 120
cgtgaaccga 130
<210> 253
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 253
gaagcgagga tcaacttggc gtgggggtgg ggtcagggaa gagggcacag ggacccaccc 60
ggtgtgtaat gtaacgcttg cctttcctct ctgcacgtcc aggctgcccc ccggtcattg 120
cgtgaaccga 130
<210> 255
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 255
gaagcgagga tcaactgtcc aggctgcccc ccggtgtgga ccccacccaa gtttcctcct 60
ccctgtcccc tgagggcaca ctgaccgtgg aggcccccat gcccaagcta gccaccattg 120
cgtgaaccga 130
<210> 255
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 255
gaagcgagga tcaactccat gcccaagcta gccacgcagt ccaacgagat caccatccca 60
gtcaccttcg agtcgcgggc ccagcttggg ggcccagaag ctgcaaaatc cgatgcattg 120
cgtgaaccga 130
<210> 256
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 256
gaagcgagga tcaactgaag ctgcaaaatc cgatgagact gccgccaagt aaagccttag 60
cccggatgcc cacccctgct gccgccactg gctgtgcctc ccccgccacc tgtgtcattg 120
cgtgaaccga 130
<210> 257
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 257
gaagcgagga tcaactcctc ccccgccacc tgtgtgttct tttgatacat ttatcttctg 60
tttttctcaa ataaagttca aagcaaccac ctgtcactgg cccaggccct ggtgtcattg 120
cgtgaaccga 130
<210> 258
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 258
gaagcgagga tcaactacct aagtcatctc agaattatac atatattgac tttttaattc 60
acatagaatc tggaactcaa ctggttggtt ggttggtgat ggggttgacc tgattcattg 120
cgtgaaccga 130
<210> 259
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 259
gaagcgagga tcaacttgat ggggttgacc tgatttcggg agaattattc ctgaaataat 60
taaggaaatg ggggttcaat ctttcttctt agctgcttgt tcctccccag cacctcattg 120
cgtgaaccga 130
<210> 260
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 260
gaagcgagga tcaactttgt tcctccccag caccttcaac tttcttctcc tcctcttcag 60
cttctttggt ttcctctcct tcttcacctt cacctccttc ttcttcttct tttgccattg 120
cgtgaaccga 130
<210> 261
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 261
gaagcgagga tcaactcttc ttcttcttct tttgcttctt cagactcttc cttggcagct 60
ttaacataaa aagaaaatgt acaaaatgca aatccagggt aagtccagtc aaaaccattg 120
cgtgaaccga 130
<210> 262
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 262
gaagcgagga tcaactgggt aagtccagtc aaaacatgtc tgcaaaggtt ttatttgcaa 60
acacaactgt cagaatgcta ccaaaacctc ccagctccac tctctccaaa agcaccattg 120
cgtgaaccga 130
<210> 263
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 263
gaagcgagga tcaactagaa aatttattta tgattctaat gtcatatatg aataaatgag 60
caaggcttca tttgtcttat ccacctccta acaaatctcc acacccagtt tacaccattg 120
cgtgaaccga 130
<210> 264
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 264
gaagcgagga tcaactctcc acacccagtt tacacttgaa gttgcagggg ttttttctta 60
tcataccttc ttcctcttca gctgcctcct cttcctcggc ctcttccttg tccttcattg 120
cgtgaaccga 130
<210> 265
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 265
gaagcgagga tcaactcggc ctcttccttg tccttctcct cctcctcggc ttctccttca 60
gagggggggc tcatccttgg cttcctcagc cttggcagcc tcaatggttt cctcccattg 120
cgtgaaccga 130
<210> 266
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 266
gaagcgagga tcaactagcc tcaatggttt cctccacttc gatctgctcc tcttggacat 60
ggctggtgta gtaggacggg aaggagcggg tggacatcag ataggagctg gtctgcattg 120
cgtgaaccga 130
<210> 267
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 267
gaagcgagga tcaacttcag ataggagctg gtctgtaaac cgccgtaggc agatcggcca 60
aagacctggg agctctggga gtagccactg gttatgcttc ccacgctggt gaaaccattg 120
cgtgaaccga 130
<210> 268
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 268
gaagcgagga tcaactcttc ccacgctggt gaaactgagt cgggtctcct cgccttccaa 60
gagtttcctg gggatgcaga tgcaaggtga ggttaaaaaa cacctgtgtt tcacacattg 120
cgtgaaccga 130
<210> 269
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 269
gaagcgagga tcaactaaaa cacctgtgtt tcacaacttg ctctgagtac cctaaacaca 60
aaggcaagca ggagcagggc tgggatttat caatacttaa tgcctgggga tctttcattg 120
cgtgaaccga 130
<210> 270
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 270
gaagcgagga tcaacttacc ctcttcttgg aagaagccaa aaactaacac ttcattattt 60
caaaaggact attattgaag gtttctgatc caacataact ttaaatggga tctgacattg 120
cgtgaaccga 130
<210> 271
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 271
gaagcgagga tcaactaact ttaaatggga tctgattttc ttcctaaggt ttaatggctg 60
ctgtctttgc ccctctattt tcacctgtaa gctgcaatct caatatccaa agccacattg 120
cgtgaaccga 130
<210> 272
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 272
gaagcgagga tcaactatct caatatccaa agccatcttc acgttgagga ggtcttggta 60
ttcttttagg tatcgtgcca tttcactctt tgtggtcctc aattcatttt ctaatcattg 120
cgtgaaccga 130
<210> 273
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 273
gaagcgagga tcaactcctc aattcatttt ctaatttgtt gatcgtgtcc tgtttgaaga 60
caaaaataaa acaaaaaaaa aatccgagca taaatccctt ctattatttt actgtcattg 120
cgtgaaccga 130
<210> 274
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 274
gaagcgagga tcaactcctt ctattatttt actgtagtgt agttgcaaag gcctagtttc 60
gattaaaatc aaggtgcaca cctttgataa aatctctcct agaataactt cctgtcattg 120
cgtgaaccga 130
<210> 275
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 275
gaagcgagga tcaactcaag gagccaagcc ctatccctaa gagctgcgtg cagccgcacc 60
acccctggtc tccactttct gggcgcacca actccccccc ttgctcgagt ccccccattg 120
cgtgaaccga 130
<210> 276
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 276
gaagcgagga tcaactcccc ttgctcgagt cccccgcccc cctgtgtttc tggccgtgcc 60
gcacctgcat agcgctgatg tcggcgttct gcttgtcctc cagctcctgc agctgcattg 120
cgtgaaccga 130
<210> 277
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 277
gaagcgagga tcaactcctc cagctcctgc agctgcttct ccagcgcttc attcatgccc 60
cggcatgctt cgatttccag ggtcttggcc ttgagcagac gacggctctc ggacacattg 120
cgtgaaccga 130
<210> 278
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 278
gaagcgagga tcaactagac gacggctctc ggacacctcg tccttggcgg cgcgcacggc 60
gtcggtgttc ttggcggcgc tctcggtcag cacggtgaag cggctcttga accatcattg 120
cgtgaaccga 130
<210> 279
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 279
gaagcgagga tcaactgaag cggctcttga accattcctc agcgttctgc atgttcttgg 60
cggccagctt ctcgtactgc gcgcggatgt ccttgagcgc ggcggaaagg tcgggcattg 120
cgtgaaccga 130
<210> 280
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 280
gaagcgagga tcaactgcgc ggcggaaagg tcgggcttgg tcacgtccat ctccacggag 60
atctgcgcgt actggatctg cgcctgcagt tcggcgatct cctcttcgtg cacttcattg 120
cgtgaaccga 130
<210> 281
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 281
gaagcgagga tcaactatct cctcttcgtg cactttcttc agaaaagaga tttcgtccat 60
caagctgtcg atgcgcttct cgagctcggc gcgagcgagc gccgcctcgt cggcgcattg 120
cgtgaaccga 130
<210> 282
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 282
gaagcgagga tcaactgagc gccgcctcgt cggcgccttt gcgcgcttcc atcagccggc 60
cctcggcgtc ctcgcggctc agcacctcct cttcatagcg cgcctgcagg ttgcgcattg 120
cgtgaaccga 130
<210> 283
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 283
gaagcgagga tcaactagcg cgcctgcagg ttgcgcaggg tctcctccag cccttcgcgc 60
tcgccctgga gcgcctgctt ctcgttggtg gcatcttccg ccgccaggcg caggtcattg 120
cgtgaaccga 130
<210> 284
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 284
gaagcgagga tcaacttccg ccgccaggcg caggtcgcgg atctcctgct cgtacagcgc 60
ccggaagcgg gatggctcgg agtgcttctg gcgcagcacc agcagctcgg cttcccattg 120
cgtgaaccga 130
<210> 285
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 285
gaagcgagga tcaactcacc agcagctcgg cttccaggac cttgttctgc tgctccagct 60
cgtgcacgcg ctcgatgaag ctggcgaagc ggtcattgag gtcctggagc tgcgccattg 120
cgtgaaccga 130
<210> 286
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 286
gaagcgagga tcaacttgag gtcctggagc tgcgccttct cctgcgtgcg gatggacttg 60
aggtcgttgc tgatggcggc tacctggctc aggtcgaggt tctccagact gggcacattg 120
cgtgaaccga 130
<210> 287
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 287
gaagcgagga tcaactaggt tctccagact gggcatcaac gatccagagc tggaggagta 60
gctgcggcgc acggacagcg aggaagacac cggcgccgag tagctggagt aagctcattg 120
cgtgaaccga 130
<210> 288
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 288
gaagcgagga tcaactcgag tagctggagt aagctgagcg tgcggtgctg tagccgctgc 60
gcacgctgga gatgtgcacc cggggcgtct ccacgtagcg ccgcttgtag gaggtcattg 120
cgtgaaccga 130
<210> 289
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 289
gaagcgagga tcaactagcg ccgcttgtag gaggtcgagt agtacggctc gtagctgaag 60
gaactcatgg tggcggccgg tggctccccg gcccgcggcg gcggtgggag cccggcattg 120
cgtgaaccga 130
<210> 290
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 290
gaagcgagga tcaactggcg gcggtgggag cccggagaga gaggacaggg gagagaggga 60
agggggagga tggatggctg tgtgcggctc ggcgccgttc tgccacccct atttacattg 120
cgtgaaccga 130
<210> 291
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 291
gaagcgagga tcaactcttg cggggcagtg tgggagggag aagtccaggg cggacaggct 60
gggcgcaccc gtgctcgcgc accccaagat ggctgagagg caggaagagc agagacattg 120
cgtgaaccga 130
<210> 292
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 292
gaagcgagga tcaactgagg caggaagagc agagagggag cccgcccttg agggcggaag 60
gcaaggccga cgcggaggtt aagctcattc tgtaccattg gacgcattcg ttcagcattg 120
cgtgaaccga 130
<210> 293
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 293
gaagcgagga tcaactattg gacgcattcg ttcagctctc aaaaggtaca acaggccttg 60
gcggcggagg gtggcgcgga tcgggcttca gcactgggac agctccttct gagtccattg 120
cgtgaaccga 130
<210> 294
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 294
gaagcgagga tcaactggac agctccttct gagtcccgcc cagggaagcc ggtccaccta 60
gaaatccgcc tccagtgtcc tgaccccggg caggcgctcc ctccaggcgg ggacgcattg 120
cgtgaaccga 130
<210> 295
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 295
gaagcgagga tcaactctta cttgttaatt ccaggaatag gaattgttaa aacgactgtt 60
gaaaatgtcg gtaacacagg gaagcccaga aaggctgctt agcggtgtcc agggacattg 120
cgtgaaccga 130
<210> 296
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 296
gaagcgagga tcaactgctt agcggtgtcc agggaagtca tttaattgaa agcttacatg 60
tgttgtagta accagtgtga actcttccag gtgcgcttgg taattgctga aaaggcattg 120
cgtgaaccga 130
<210> 297
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 297
gaagcgagga tcaactttgg taattgctga aaaggcattg aagtgcgagg aacatgatgt 60
aagtctgccc ttgagtgagc acaatgagcc ttggtttatg cgtttgaact caactcattg 120
cgtgaaccga 130
<210> 298
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 298
gaagcgagga tcaacttatg cgtttgaact caactggaga agtgcctgtc cttatccacg 60
gggaaaacat aatttgtgag gccactcaga tcattgatta tcttgaacag actttcattg 120
cgtgaaccga 130
<210> 299
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 299
gaagcgagga tcaactatta tcttgaacag actttcctgg atggtaatgt taaggctact 60
tgcgatttct tggatttact ttcaacacaa ctatgtgttc cctttctctt tttctcattg 120
cgtgaaccga 130
<210> 300
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 300
gaagcgagga tcaactgttc cctttctctt tttctctctc tcctctctct ctttctcttg 60
tgtcatgatg gtggtttggg attaaaaacc ttttccattt ccataagcac acaaacattg 120
cgtgaaccga 130
<210> 301
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 301
gaagcgagga tcaactgttt tttgaatata cagatatgtt gaaaatgtta atgatgagtg 60
gatagtgttt ttgttttgct tttgagtgta acaactcatg tgtaactttt tcttccattg 120
cgtgaaccga 130
<210> 302
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 302
gaagcgagga tcaactcatg tgtaactttt tcttcaatat ttgtgtgtgt gtattttaga 60
aagaacaccc aggttaatgc ctgataaaga aagcatgtat tacccacggg tacaacattg 120
cgtgaaccga 130
<210> 303
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 303
gaagcgagga tcaactgtat tacccacggg tacaacatta ccgagagctg cttgactcct 60
tgccaatgga tgcctataca catggctgca ttttacatcc tgagttaact gtggacattg 120
cgtgaaccga 130
<210> 304
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 304
gaagcgagga tcaactatcc tgagttaact gtggactcca tgatcccggc ttatgcaact 60
acaaggattc gtagtatgta aacattttaa agacctggaa ttctgtctga cacttcattg 120
cgtgaaccga 130
<210> 305
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 305
gaagcgagga tcaactggaa ttctgtctga cactttcttt taattcattt tgagacctag 60
catgtctcat ggtcactaaa agaagattgg gaatagggtt gttgagtttg cttcacattg 120
cgtgaaccga 130
<210> 306
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 306
gaagcgagga tcaactgcag agaagcaggg catgagcccc agagctcatg ctcttgtcat 60
tgagtttctc tgcttctcct tgttactggt gtagaaggga gaaaataatt ttctgcattg 120
cgtgaaccga 130
<210> 307
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 307
gaagcgagga tcaactggga gaaaataatt ttctgtttcc aaaatgtttt tattatcagg 60
ccaaattgga aacacagagt ctgagctgaa gaaacttgct gaagaaaacc cagatcattg 120
cgtgaaccga 130
<210> 308
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 308
gaagcgagga tcaacttgct gaagaaaacc cagatttaca agaagcatac attgcaaaac 60
agaaacgact taaagtaagc caatcagctg tcctcagttg acatacactg cacggcattg 120
cgtgaaccga 130
<210> 309
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 309
gaagcgagga tcaactgttg acatacactg cacggagtaa atgttctact ttttgtaaag 60
taccactatt tctagaatat cttcttttaa atattgcagt tcaccagtac acatgcattg 120
cgtgaaccga 130
<210> 310
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 310
gaagcgagga tcaacttttt tttttttttt tttttgctga gtttttctat ttcttctcgt 60
tgtctaaaat aggctgaact ctgtaagagt ttgtatctgc tttacctaga tttatcattg 120
cgtgaaccga 130
<210> 311
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 311
gaagcgagga tcaactctgc tttacctaga tttatttttt ttaaaggtgc aaataatatg 60
tttggtttct ttttttggtg gttaattagt caaagctgct tgatcatgac aatgtcattg 120
cgtgaaccga 130
<210> 312
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 312
gaagcgagga tcaacttgct tgatcatgac aatgtcaagt atttgaagaa aattcttgat 60
gagttggaga aagtcttgga tcaggttgaa actgaattgc aaagaagaaa tgaagcattg 120
cgtgaaccga 130
<210> 313
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 313
gaagcgagga tcaactttgc aaagaagaaa tgaagaaacc ccaggtaggt tctcatttat 60
attctttctc tcttttcaac atcagtatta ttcatgggaa cattcttagg tctcacattg 120
cgtgaaccga 130
<210> 314
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 314
gaagcgagga tcaactggaa cattcttagg tctcaccaaa aacgttctgt aatctgcttt 60
tcattgttgt tattcacagg cagttttaat tatgatagca ataatgatat tgcaccattg 120
cgtgaaccga 130
<210> 315
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 315
gaagcgagga tcaacttgtc aagaaaataa atatataatg tccttcatct tttgctatac 60
tcacactcac ccttaagggt gagaccactg ataccagctg gagtctgtct gtagacattg 120
cgtgaaccga 130
<210> 316
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 316
gaagcgagga tcaactgctg gagtctgtct gtagagtgct tgcctctaat tctctatgtc 60
cctttctcta attagaagag ggccagcaac cttggctctg cggtgaatcc ttcaccattg 120
cgtgaaccga 130
<210> 317
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 317
gaagcgagga tcaacttctg cggtgaatcc ttcaccctgg cagacgtctc actcgctgtc 60
acattgcatc gactgaagtt cctggggttt gcaaggagaa actggggaaa cggaacattg 120
cgtgaaccga 130
<210> 318
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 318
gaagcgagga tcaactagaa actggggaaa cggaaagcga ccaaacttgg aaacctatta 60
cgagcgtgtc ttgaagagaa aaacatttaa caaggtttta ggacatgtca acaatcattg 120
cgtgaaccga 130
<210> 319
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 319
gaagcgagga tcaactttta ggacatgtca acaatatatt aatctctgca gtgctgccaa 60
cagcattccg ggtggccaag aaaagggccc caaaagttct tggcacgacc cttgtcattg 120
cgtgaaccga 130
<210> 320
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 320
gaagcgagga tcaactttct tggcacgacc cttgtggttg gtttgcttgc aggagtggga 60
tattttgctt ttatgctttt cagaaagagg cttggcagca tgatattagc atttacattg 120
cgtgaaccga 130
<210> 321
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 321
gaagcgagga tcaactagca tgatattagc atttagaccc agaccaaatt atttctaggt 60
ttgttgggat cttgtcgtgg cagctcatcc aagcatttag ctagaccctg tgattcattg 120
cgtgaaccga 130
<210> 322
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 322
gaagcgagga tcaactttag ctagaccctg tgattgcccg tggctctctg agtctgtctt 60
attgagtagt tagcagtatt ttttcctaaa attcagaagt catctttgtt acacacattg 120
cgtgaaccga 130
<210> 323
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 323
gaagcgagga tcaacttaat tctcagttta ggcctttgca tatctgatat attaagacaa 60
aagcaaaaac aaatatttgc agtgtattca gttttgtcaa tggaagcaat tgattcattg 120
cgtgaaccga 130
<210> 324
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 324
gaagcgagga tcaacttcaa tggaagcaat tgatttccat cattaaatga taaaaagcca 60
cttcaatgaa gaaatgattt ttcagacctt ctcatagatt ctggtgcaca ccacgcattg 120
cgtgaaccga 130
<210> 325
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 325
gaagcgagga tcaactgatt ctggtgcaca ccacgccctt cattttacat tccttaaaaa 60
agagagaggt taattgagta tctcaagttc aaaccagaga acagaaaatt tgtaacattg 120
cgtgaaccga 130
<210> 326
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 326
gaagcgagga tcaactgaga acagaaaatt tgtaataaaa atagaaattc aaaataaact 60
cataagatta atttagaaag gaataaaata tcagtatgtc agtctccagt aattccattg 120
cgtgaaccga 130
<210> 327
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 327
gaagcgagga tcaacttctt actttattct tagtcatttt tatccattca tattccaaag 60
aacaaagaat attttgaaaa caatataatc aaattattta tatagcatta tttctcattg 120
cgtgaaccga 130
<210> 328
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 328
gaagcgagga tcaactttta tatagcatta tttctctatt tagaagtaaa gatactctta 60
ccgctaccat tttcccattc actagctttc tctttatggt tgtctctttg ccatccattg 120
cgtgaaccga 130
<210> 329
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 329
gaagcgagga tcaacttggt tgtctctttg ccatcccatc tctgcacttg attcagtgat 60
cctctctgca gggttacgat gctctgcaaa gacaaggtca tgcaattgac ttgcccattg 120
cgtgaaccga 130
<210> 330
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 330
gaagcgagga tcaactgtca tgcaattgac ttgcctgaaa tttgttgatc aaagaagcag 60
ttctttcagt atggaaccat tttcataata gatatagatg tctcaggagg tacaccattg 120
cgtgaaccga 130
<210> 331
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 331
gaagcgagga tcaactgatg tctcaggagg tacactcctt ttcaacagaa ttgagtctac 60
ccttttacac tagcaggaat attcctctct ctcaagcagc ccactgggcc aacttcattg 120
cgtgaaccga 130
<210> 332
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 332
gaagcgagga tcaactcagc ccactgggcc aactttgaag agaaacatta aagattacct 60
tggtctttct attgtcagct gtggtttctt caaattcctg gcctagcttg aaggacattg 120
cgtgaaccga 130
<210> 333
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 333
gaagcgagga tcaactcctg gcctagcttg aaggagattt ctgtattttt aaaggtactt 60
tcagttcgta tagttataat atctcctttc ttgctgatga tcacagtggg tttggcattg 120
cgtgaaccga 130
<210> 334
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 334
gaagcgagga tcaactatga tcacagtggg tttggccaaa tttcccagtt ttctggtggc 60
taaccccaca cctgaaaatt aagatagtgt tagaattatg ttgaccaaga gagcacattg 120
cgtgaaccga 130
<210> 335
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 335
gaagcgagga tcaacttatg ttgaccaaga gagcatagcc aaagagactg tggtggccac 60
atcctacgct cagtggtgcc ccaggtgagt tgatggcagg taatatcact tactacattg 120
cgtgaaccga 130
<210> 336
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 336
gaagcgagga tcaactgatt aatcctaaaa gaggattgta ctgcagcctt gcaaaactcc 60
ccagtatcct gttaaaagta gtagatgaaa atgtcatgta gctttcttaa aaaggcattg 120
cgtgaaccga 130
<210> 337
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 337
gaagcgagga tcaacttgta gctttcttaa aaaggagctt ttcagcagaa caacaaaaag 60
catttcttac ccagagcttt catgtaatcg tcaaagttct cactagagac aagttcattg 120
cgtgaaccga 130
<210> 338
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 338
gaagcgagga tcaactttct cactagagac aagtttccag gtgcccagga atttgttgct 60
catcgtgatg ggtgagagct caacacagtt ctaagtggga ttcagaagac tcagacattg 120
cgtgaaccga 130
<210> 339
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 339
gaagcgagga tcaactggga ttcagaagac tcagataaaa tgctgaggcc tcagaagatt 60
cttttcaagg atgcccaaag cgtgtgactc ttacagagac ccaatgggga aaggccattg 120
cgtgaaccga 130
<210> 340
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 340
gaagcgagga tcaacttaat gacatctgca ttgtattttc tagctgaagc aaaacagtgc 60
ctgtgggatt tatgtgactt cttaaccttg aagtccattc atagaaccct agcttcattg 120
cgtgaaccga 130
<210> 341
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 341
gaagcgagga tcaactattc atagaaccct agcttccttt tcacagggag gagctgtgtg 60
atgaagcagg caggattaca ggccactcct ttacccaggg aagcaactgc gatggcattg 120
cgtgaaccga 130
<210> 342
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 342
gaagcgagga tcaactaggg aagcaactgc gatggtataa gagcgaggtc caccaatctc 60
ctgccggttt atcatagcta cagcccaggc taagcctgag agaggtcgtt cccaccattg 120
cgtgaaccga 130
<210> 343
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 343
gaagcgagga tcaacttgag agaggtcgtt cccacacttc aaagttgtct ccctgaaaaa 60
ccaagaaagt gtggttgctt agcaactagt gataagtggc cctgttagtt tggcacattg 120
cgtgaaccga 130
<210> 344
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 344
gaagcgagga tcaacttggc cctgttagtt tggcattcat tcttaatgtc agaactatca 60
agcacttgta ggaaaaatta aaatgatcct gtagcattat tttggaaata aagtacattg 120
cgtgaaccga 130
<210> 345
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 345
gaagcgagga tcaacttcta gcaagaagct ttattactga tagtaacatc aagagcaagg 60
gaaaaaaata gatttaggcc caagacaaag ttggtattgg gtatataaag ccatccattg 120
cgtgaaccga 130
<210> 346
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 346
gaagcgagga tcaactttgg gtatataaag ccatcttaaa atatatactc ttatttacct 60
gtctaagctg gtacccttgc ttgcccaagg ggtcctgatt gatggcaatt acgtccattg 120
cgtgaaccga 130
<210> 347
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 347
gaagcgagga tcaactgatt gatggcaatt acgtccttat cctgaaggag agctttggct 60
tgagggctga tgtgtcggag gtcattagac atgaataaag gagcagccat gatagcattg 120
cgtgaaccga 130
<210> 348
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 348
gaagcgagga tcaactaaag gagcagccat gatagcccag agggccatct gagttacttg 60
ctgattccag ctgaggccaa agttgccaat cactaactga gaaaaagaat gaaatcattg 120
cgtgaaccga 130
<210> 349
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 349
gaagcgagga tcaactctga gaaaaagaat gaaataattc aaacaagaga ggaggaaaca 60
ttcttaaagt tacctagatg acccatatgg agaaaccact ttccacagca tcctgcattg 120
cgtgaaccga 130
<210> 350
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 350
gaagcgagga tcaactcact ttccacagca tcctgctcta agtactctca cataaagcct 60
cctcccagga actttacctg tatttacctt gaatgtcaaa ataggaaaca agcctcattg 120
cgtgaaccga 130
<210> 351
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 351
gaagcgagga tcaactcaaa ataggaaaca agcctaccgc agggtcttga acaaggaggg 60
ctcaagtttt taccatatct gggtcattcc aaccccctgg tccagcaaca tcaaccattg 120
cgtgaaccga 130
<210> 352
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 352
gaagcgagga tcaactctgg tccagcaaca tcaacaattc tctcctggtt aaaagatgtc 60
cagtccaaga tactctttat acttttccag gaatcatcaa tgtcagcaaa atttccattg 120
cgtgaaccga 130
<210> 353
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 353
gaagcgagga tcaacttcaa tgtcagcaaa atttcgccag tgattgcagt actgtcggat 60
ttctgtataa ttgggctgtg aaaacagata tgactcttct gtttactttc tactacattg 120
cgtgaaccga 130
<210> 354
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 354
gaagcgagga tcaactttct gtttactttc tactaacatc cttgtgagat gaaaacagtt 60
taaaggaggc acttgtagcc ttctcttgag tttacagatt gaacgtctcc ataagcattg 120
cgtgaaccga 130
<210> 355
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 355
gaagcgagga tcaactaacc tgggagagat ggtaggatga tagtaagtaa cgttggactt 60
tgaaggagac cttggtttcc tttgttgtca agttctatct atcagtacag ttctacattg 120
cgtgaaccga 130
<210> 356
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 356
gaagcgagga tcaactatct atcagtacag ttctattgga ttctgggctc actatctcac 60
cttttgaaag ggccacatat aaagaggcca ctcacaggag tacacaatgc ttctgcattg 120
cgtgaaccga 130
<210> 357
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 357
gaagcgagga tcaactggag tacacaatgc ttctgccagt cctattcagg gccaaggaca 60
tgtgcttata acctgtatga gaaaacaatg ggtaaaataa gggaaagaaa tgaatcattg 120
cgtgaaccga 130
<210> 358
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 358
gaagcgagga tcaactataa gggaaagaaa tgaatttcca gctggggcta tatatatagt 60
tattttattt tattttattt taattttttg agatggggtt cagtctgtca tccagcattg 120
cgtgaaccga 130
<210> 359
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 359
gaagcgagga tcaactgaat aattatttcc agtattgtga cagggtattt aaaattctga 60
agattggttc tttggctcag ctaccatggc ctcaaagttc tttcctttgt ggctacattg 120
cgtgaaccga 130
<210> 360
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 360
gaagcgagga tcaactgttc tttcctttgt ggctaaatct ctggaatgaa acattaccat 60
ctgccaaatt ttccaaactg tcacagtaac aaccatcaaa ttttagcaga tctaccattg 120
cgtgaaccga 130
<210> 361
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 361
gaagcgagga tcaactcaaa ttttagcaga tctactcccc agtcagcaaa ggtctgggca 60
tcaatgtcgt agtatccaaa actcccaggg aagcctgcgc aggttttatt tccaacattg 120
cgtgaaccga 130
<210> 362
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 362
gaagcgagga tcaactgcgc aggttttatt tccaacatct gcataaatcc ctagcttcag 60
tcctttgctg tgaacctgaa atgagaggga ggaaaagagt caccattgta gaagccattg 120
cgtgaaccga 130
<210> 363
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 363
gaagcgagga tcaactgagt caccattgta gaagcacaat cgtgaggtag cagaaagaga 60
gaaccattcc aggctggggg aagagacaag gttacttcac caggtattgg gggctcattg 120
cgtgaaccga 130
<210> 364
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 364
gaagcgagga tcaactcaaa gtgttgggat tacaggcgtg agccaccacg cccggccatg 60
agggctgttt ctaaacaagc ttctgtacag aagtgcttac agtcctctga atgaacattg 120
cgtgaaccga 130
<210> 365
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 365
gaagcgagga tcaactttac agtcctctga atgaacaaga acattatcta taaactcaca 60
taattagcta gctggcgaat cccatgagga aagcgctgag ggtctgcctg aagtccattg 120
cgtgaaccga 130
<210> 366
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 366
gaagcgagga tcaacttgag ggtctgcctg aagtctgcct tctgaatctc tttggggagc 60
catccaacag tcatcaatgc agaggtactc ataacctgca tccttccagc cttctcattg 120
cgtgaaccga 130
<210> 367
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 367
gaagcgagga tcaacttgca tccttccagc cttctgagac catgagctct gccatctcca 60
tgaagagctt ctcactgaaa gagaaattcc aataatcatt acaattcatt aaatgcattg 120
cgtgaaccga 130
<210> 368
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 368
gaagcgagga tcaactcatt acaattcatt aaatgaacac ttaggtacct cccatttatt 60
aggcaccttg ggatttcaca attttttcca acccagtaat agtgtggtag taatccattg 120
cgtgaaccga 130
<210> 369
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 369
gaagcgagga tcaactgccg acttcggttc cggtctctgc agcagccgtg atcgcttagt 60
ggagtgctta gggtagttgg ccaggatgcc gaatatcaaa atcttcagcg gcagccattg 120
cgtgaaccga 130
<210> 370
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 370
gaagcgagga tcaactcaaa atcttcagcg gcagctccca ccaggactta tctcagaaaa 60
ttgctgaccg cctgggcctg gagctaggca aggtggtgac taagaaattc agcaacattg 120
cgtgaaccga 130
<210> 371
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 371
gaagcgagga tcaacttgac taagaaattc agcaaccagg agacctggta aggaccaagt 60
tgggaccctg actagacctc acctgcccag gcggcagagt ataggaggga tggggcattg 120
cgtgaaccga 130
<210> 372
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 372
gaagcgagga tcaactgagt ataggaggga tggggtcgcc gcttgagctc aaacagactg 60
ggggttgggg ggagagggtg cagctctgtg actagcctac cccacgggct gtttccattg 120
cgtgaaccga 130
<210> 373
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 373
gaagcgagga tcaactgaaa aaaatctgta catgttcaat acaggtgcaa ttttttctcc 60
aaatattttc aatccacact tggttgaatc catagatgtg gaacctatgg atatgcattg 120
cgtgaaccga 130
<210> 374
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 374
gaagcgagga tcaacttgtg gaacctatgg atatggaggg ctgacagtac agtggtttaa 60
aatctatttc atgttctttc tttcctcatt gtagtgtgga aattggtgaa agtgtcattg 120
cgtgaaccga 130
<210> 375
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 375
gaagcgagga tcaacttgga aattggtgaa agtgtacgtg gagaggatgt ctacattgtt 60
cagagtggtt gtggcgaaat caatgacaat ttaatggagc ttttgatcat gattacattg 120
cgtgaaccga 130
<210> 376
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 376
gaagcgagga tcaactgagc ttttgatcat gattaatgcc tgcaagattg cttcagccag 60
ccgggttact gcagtcatcc catgcttccc ttatgcccgg caggataaga aagatcattg 120
cgtgaaccga 130
<210> 377
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 377
gaagcgagga tcaactccgg caggataaga aagataaggt aggagcagaa ttttattttt 60
tgagcagagg aagcaggagc acttgcccca aatcacaaat aaattccaaa attatcattg 120
cgtgaaccga 130
<210> 378
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 378
gaagcgagga tcaactaaat aaattccaaa attatcaaat atatatttag ggggaatttt 60
aaaaatcacc ttttcttagg tattacccta tcttcaaaac aatagaaata taattcattg 120
cgtgaaccga 130
<210> 379
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 379
gaagcgagga tcaactaatt tctgggtacc atagtgcctt taacatagta ggtacacaat 60
aaatagtttc ttgagtaaat gaattgtttt tcaaattggc tttttggttt ttcttcattg 120
cgtgaaccga 130
<210> 380
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 380
gaagcgagga tcaacttggc tttttggttt ttcttttctt tcctcccctc catttagagc 60
cgggcgccaa tctcagccaa gcttgttgca aatatgctat ctgtagcagg tgcagcattg 120
cgtgaaccga 130
<210> 381
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 381
gaagcgagga tcaactctat ctgtagcagg tgcagatcat attatcacca tggacctaca 60
tgcttctcaa attcaggtat cagtggaagc taaatattgg tgtttgaaag gtgggcattg 120
cgtgaaccga 130
<210> 382
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 382
gaagcgagga tcaactttgg tgtttgaaag gtgggaggaa aggactgatg atgctgaaga 60
ctgcagagaa gccaaaaatt atgcccaagt acatctgaaa taaactagat atcaacattg 120
cgtgaaccga 130
<210> 383
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 383
gaagcgagga tcaacttttt ataatctacc acactgggcc tgccttccca tcagtttgaa 60
tgttgcagta agacattctc tgagcaagtt actactttgt gtttgccatt tataacattg 120
cgtgaaccga 130
<210> 384
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 384
gaagcgagga tcaactttgt gtttgccatt tataaatgga agagaaggaa agtgaagcaa 60
aactgatcca gcttctttct acagggcttt tttgatatcc cagtagacaa tttgtcattg 120
cgtgaaccga 130
<210> 385
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 385
gaagcgagga tcaactatcc cagtagacaa tttgtatgca gagccggctg tcctaaagtg 60
gataagggag aatatctctg agtggaggaa ctgcactatt gtctcacctg atgctcattg 120
cgtgaaccga 130
<210> 386
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 386
gaagcgagga tcaacttatt gtctcacctg atgctggtgg agctaagagg tatggttgaa 60
attagtattg ttcccaatgt actgggaaaa tctttcagat ggttgtgtca caaagcattg 120
cgtgaaccga 130
<210> 387
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 387
gaagcgagga tcaactagat ggttgtgtca caaagtgaag tttttctatc caagtggcag 60
tttttaagta tttttgaatg gatgtaagta gctagcacat gggttgaata tagttcattg 120
cgtgaaccga 130
<210> 388
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 388
gaagcgagga tcaactcatg accatccttc ctttcctgct actggctctt ggaagagttg 60
agggggggtg cgcaggcagt gctatggcgc ccgactttcc accccagctt tctgacattg 120
cgtgaaccga 130
<210> 389
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 389
gaagcgagga tcaactttcc accccagctt tctgacagct tgcttcatgg ctggtgtttt 60
gcaggtgtga atgaggcagg atgaactgga caggtttgta caccttgctc agtggcattg 120
cgtgaaccga 130
<210> 390
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 390
gaagcgagga tcaacttgta caccttgctc agtggcgtga accggcattc tactgccatt 60
ggccgagtat ggctctcggt catcttcatc ttcagaatca tggtgctggt ggtggcattg 120
cgtgaaccga 130
<210> 391
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 391
gaagcgagga tcaactatca tggtgctggt ggtggctgca gagagtgtgt ggggtgatga 60
gaaatcttcc ttcatctgca acacactcca gcctggctgc aacagcgttt gctatcattg 120
cgtgaaccga 130
<210> 392
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 392
gaagcgagga tcaactctgc aacagcgttt gctatgacca attcttcccc atctcccatg 60
tgcggctgtg gtccctgcag ctcatcctag tttccacccc agctctcctc gtggccattg 120
cgtgaaccga 130
<210> 393
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 393
gaagcgagga tcaactcccc agctctcctc gtggccatgc acgtggctca ccagcaacac 60
atagagaaga aaatgctacg gcttgagggc catggggacc ccctacacct ggaggcattg 120
cgtgaaccga 130
<210> 394
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 394
gaagcgagga tcaactgacc ccctacacct ggaggaggtg aagaggcaca aggtccacat 60
ctcagggaca ctgtggtgga cctatgtcat cagcgtggtg ttccggctgt tgtttcattg 120
cgtgaaccga 130
<210> 395
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 395
gaagcgagga tcaactggtg ttccggctgt tgtttgaggc cgtcttcatg tatgtctttt 60
atctgctcta ccctggctat gccatggtgc ggctggtcaa gtgcgacgtc taccccattg 120
cgtgaaccga 130
<210> 396
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 396
gaagcgagga tcaacttcaa gtgcgacgtc tacccctgcc ccaacacagt ggactgcttc 60
gtgtcccgcc ccaccgagaa aaccgtcttc accgtcttca tgctagctgc ctctgcattg 120
cgtgaaccga 130
<210> 397
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 397
gaagcgagga tcaactttca tgctagctgc ctctggcatc tgcatcatcc tcaatgtggc 60
cgaggtggtg tacctcatca tccgggcctg tgcccgccga gcccagcgcc gctcccattg 120
cgtgaaccga 130
<210> 398
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 398
gaagcgagga tcaactccga gcccagcgcc gctccaatcc accttcccgc aagggctcgg 60
gcttcggcca ccgcctctca cctgaataca agcagaatga gatcaacaag ctgctcattg 120
cgtgaaccga 130
<210> 399
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 399
gaagcgagga tcaactatga gatcaacaag ctgctgagtg agcaggatgg ctccctgaaa 60
gacatactgc gccgcagccc tggcaccggg gctgggctgg ctgaaaagag cgacccattg 120
cgtgaaccga 130
<210> 400
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 400
gaagcgagga tcaactctgg ctgaaaagag cgaccgctgc tcggcctgct gatgccacat 60
accaggcaac ctcccatccc acccccgacc ctgccctggg cgagcccctc cttctcattg 120
cgtgaaccga 130
<210> 401
<211> 130
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 401
gaagcgagga tcaacttggg cgagcccctc cttctcccct gccggtgcac aggcctctgc 60
ctgctgggga ttactcgatc aaaaccttcc ttccctggct acttcccttc ctccccattg 120
cgtgaaccga 130
Claims (6)
1.一种用于对目标基因进行SNP/Indel变异检测的探针组合的设计方法，其特征在于，包括以下步骤：
S1，将目标基因的每个外显子及其两端50bp作为第一探针区间，针对至少每个所述第一探针区间设计一个或多个探针；
S2，针对目标基因除所述第一探针区间以外的区域设计探针：以100bp为滑动窗口，当窗口中有2个以上致病突变位点时，将该窗口作为第二探针区间，针对至少一个所述第二探针区间设计一个或多个探针；
S3，若S1～S2设计的探针GC含量高于80％的，若探针靶向的探针区间具有人群突变频率为20％～80％的SNP位点，则将突变碱基作为探针碱基，若无相关突变位点的，则随机将探针中的5个G或C碱基改变为A或T，
针对存在CNV的目标基因，每间隔1～3k，设置1个200bp第三探针区间，并针对至少一个所述第三探针区间设计一个或多个CNV-plus探针，
设计所述CNV-plus探针的具体步骤如下：
S1’，距离目标基因起始位置1～3k，选取一个200bp的区间，若该区间同时满足：(1)包含参考人类基因数据库中人群突变频率为20％～80％的SNP位点，且(2)在基因组上无相似序列，则将该区间作为第一个第三探针区间；
S2’，在选择的第一个第三探针区间之后，在基因上间隔1～3k内再次选取一个200bp的区间，若该区间同时满足S1’中(1)和(2)的条件，则将该区间作为第二个第三探针区间，若距离第一个第三探针区间1～3k内没有符合S1’中(1)和(2)所述要求的区间，则在距离第一个第三探针区间2k位置处选取200bp区间作为第二个第三探针区间，依此类推，直到最后一个第三探针区间后没有同时满足S1’中(1)和(2)的条件的区间且距离基因终止位置不足2k；
S3’，针对得到的至少一个所述第三探针区间设计一个或多个探针，
所述目标基因包括PMP22、GJB1、MFN2、MPZ、GDAP1、MORC2、SORD、NEFH、HSPB1、NEFL、PMP2、GLA、PRPS1、TTR，所述存在CNV的目标基因为PMP22。
2.一种用于诊断腓骨肌萎缩症的探针组合，其特征在于，包括利用权利要求1所述的设计方法设计得到的靶向目标基因的探针组合。
3.根据权利要求2所述的用于诊断腓骨肌萎缩症的探针组合，其特征在于，靶向PMP22基因的探针组合包括SEQ ID No.87～129所示的探针；靶向GJB1基因的探针组合包括SEQID No.388～401所示的探针；靶向MFN2基因的探针组合包括SEQ IDNo.1～10所示的探针；靶向MPZ基因的探针组合包括SEQ ID No.11～20所示的探针；靶向GDAP1基因的探针组合包括SEQ ID No.291～322所示的探针；靶向MORC2基因的探针组合包括SEQ ID No.220～241所示的探针；靶向SORD基因的探针组合包括SEQ ID No.21～40所示的探针；靶向NEFH基因的探针组合包括SEQ ID No.170～219所示的探针；靶向HSPB1基因的探针组合包括SEQ IDNo.242～257所示的探针；靶向NEFL基因的探针组合包括SEQ ID No.258～290所示的探针；靶向PMP2基因的探针组合包括SEQ ID No.323～339所示的探针；靶向GLA基因的探针组合包括SEQ IDNo.340～368所示的探针；靶向PRPS1基因的探针组合包括SEQ ID No.369～387所示的探针；靶向TTR基因的探针组合包括SEQ ID No.151～169所示的探针，
还包括用于分析PMP22基因拷贝数变异的探针组合，包括靶向chr17:14100991-14101127、chr17:14133516-14133640、chr17:14171563-14171695、chr17:14235680-14235802、chr17:14285014-14285146、chr17:14312221-14312359、chr17:14343889-14344015、chr17:14409890-14410024、chr17:14457954-14458086、chr17:14492312-14492444、chr17:14531113-14531265、chr17:14596178-14596308、chr17:14646877-14646999、chr17:14655667-14655843、chr17:14735456-14735580、chr17:14780118-14780242、chr17:14800953-14801085、chr17:14850608-14850734、chr17:14898767-14898911、chr17:14939853-14939975、chr17:14979111-14979257、chr17:15018350-15018500、chr17:15065290-15065416、chr17:15133696-15133826、chr17:15136057-15136181、chr17:15141300-15141426、chr17:15142280-15142454、chr17:15144794-15144916、chr17:15148220-15148342、chr17:15150538-15150664、chr17:15152985-15153107、chr17:15155327-15155469、chr17:15157779-15157901和chr17:15158695-15158833的一个或多个探针。
4.一种用于诊断腓骨肌萎缩症的试剂盒，其特征在于，所述试剂盒包括权利要求2～3任一所述的用于诊断腓骨肌萎缩症探针组合。
5.根据权利要求4所述的用于诊断腓骨肌萎缩症的试剂盒，其特征在于，所述试剂盒还包括基因组DNA提取试剂、末端修复试剂、末端加“A”试剂、核酸扩增试剂和/或纯化试剂。
6.权利要求2～3任一所述的用于探针组合在制备适用于以下方法诊断腓骨肌萎缩症的试剂盒中的应用，其特征在于，所述方法包括以下步骤：
S101，获得个体的基因组DNA样本；
S102，利用所述探针组合进行富集并进行高通量测序；
S103，根据高通量测序数据诊断个体是否患有腓骨肌萎缩症。
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2015011262A1 (en) * | 2013-07-26 | 2015-01-29 | INSERM (Institut National de la Santé et de la Recherche Médicale) | In vitro genetic diagnostic of inherited peripheral neuropathies (charcot-marie-tooth disease) |
| CN106011224A (zh) * | 2015-12-24 | 2016-10-12 | 晶能生物技术（上海）有限公司 | 神经系统遗传性疾病基因联合筛查方法、试剂盒及其制备方法 |
| CN107529555A (zh) * | 2017-04-21 | 2018-01-02 | 上海杰傲奉生医学检验所有限公司 | 腓骨肌萎缩症相关pmp22基因拷贝数变异检测试剂盒 |
| KR20180105597A (ko) * | 2018-08-13 | 2018-09-28 | 사회복지법인 삼성생명공익재단 | 샤르코-마리-투스 질환 진단용 키트 |
| CN108753954A (zh) * | 2018-06-26 | 2018-11-06 | 中南大学湘雅医院 | 痴呆相关基因的捕获探针组、试剂盒、文库构建方法和用途 |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015011262A1 (en) * | 2013-07-26 | 2015-01-29 | INSERM (Institut National de la Santé et de la Recherche Médicale) | In vitro genetic diagnostic of inherited peripheral neuropathies (charcot-marie-tooth disease) |
| CN106011224A (zh) * | 2015-12-24 | 2016-10-12 | 晶能生物技术（上海）有限公司 | 神经系统遗传性疾病基因联合筛查方法、试剂盒及其制备方法 |
| CN107529555A (zh) * | 2017-04-21 | 2018-01-02 | 上海杰傲奉生医学检验所有限公司 | 腓骨肌萎缩症相关pmp22基因拷贝数变异检测试剂盒 |
| CN108753954A (zh) * | 2018-06-26 | 2018-11-06 | 中南大学湘雅医院 | 痴呆相关基因的捕获探针组、试剂盒、文库构建方法和用途 |
| KR20180105597A (ko) * | 2018-08-13 | 2018-09-28 | 사회복지법인 삼성생명공익재단 | 샤르코-마리-투스 질환 진단용 키트 |
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