CN114272287B - Traditional Chinese medicine composition for promoting growth and preparation method thereof - Google Patents
Traditional Chinese medicine composition for promoting growth and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine composition for promoting growth, which is prepared from poria cocos, astragalus mongholicus, radix salviae miltiorrhizae and broussonetia papyrifera leaves. The Chinese medicinal composition has effects of invigorating spleen and qi, promoting growth, and improving production performance. Compared with antibiotics, hormone and chemical synthetic drugs, the traditional Chinese medicine composition disclosed by the invention can prevent drug residues in vivo, has no toxic or side effect, is safe and reliable, and is suitable for promoting growth of livestock and poultry.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese veterinary medicines, and in particular relates to a traditional Chinese medicine composition for promoting growth and improving production performance of livestock and poultry animals and a preparation method thereof.
Background
According to the requirements of 194 th bulletin of agricultural rural area, all growth-promoting medicine feed additive varieties (except traditional Chinese medicines) are withdrawn from 1 month and 1 day in 2020, growth-promoting medicines are forbidden to be used as growth-promoting agents in the feed, and the breeding industry faces the problems of how to effectively improve animal production performance, feed conversion rate and the like. The decrease in animal growth performance will directly result in an increase in feeding costs. The national agricultural rural department issues opinion on promoting the healthy development of veterinary medicine industry, which clearly proposes the research and development of traditional Chinese veterinary medicines and medicine feed additives for enhancing the curative effect, and supports the creation of feed antibiotics to replace products. Therefore, the novel growth-promoting additive is urgently needed to be developed in China as a large country of the breeding industry, and has wide market space.
China has rich advantages of Chinese veterinary medicine resources and a solid research basis. In the thousands of calendar Shi Chang river, traditional Chinese veterinary medicines play a vital role in preventing and treating diseases of livestock and poultry. The middle veterinarian theory holds that the yin-yang balance of the organism can be maintained in a normal state, and if yin is excessive and yang is deficient, or yang is excessive and yin is deficient, the disease is serious; the balance of yin and yang is regulated by four qi and five flavors of traditional Chinese veterinary medicines, and the disease is eliminated. In the modern breeding process, the health growth of livestock and poultry is ensured to be more and more valued by farmers by utilizing traditional Chinese veterinary medicines, and the popularization of the traditional Chinese veterinary medicines is also wider and wider.
Polysaccharide is one of the effective component tables of Chinese herbal medicines, is a natural polymer compound formed by monosaccharide polymerization, and is one of basic substances for maintaining normal operation of living organisms. As people have conducted intensive researches on polysaccharides, it has been found that polysaccharides have many bioactive effects, and have good effects in resisting tumor, virus, oxidation, immunity, inflammation, blood sugar and blood lipid, aging, promoting fracture healing, etc. The most prominent and common function of polysaccharides is the enhancement of immune function of the body, and this function is exerted mainly by the following pathways: activating macrophages, activating T cells and B lymphocytes, and effects on various cytokines. The polysaccharide growth promoting effect was found to be closely related to its immunomodulatory effect. The polysaccharide can reduce nutrient substances consumed by the organism for supporting immune response by enhancing the immune barrier of the organism, and reduce the inhibition effect of products in immune response on feeding and growth, thereby promoting the growth of animals.
Therefore, a traditional Chinese medicine product capable of effectively promoting growth and development is developed, so that the traditional Chinese medicine product meets the requirements of no toxicity, no harm, no side effect and environmental protection, and has important significance.
Disclosure of Invention
Through intensive and careful experiments, the invention successfully develops a traditional Chinese medicine composition for promoting growth and improving production performance, and the curative effect of the traditional Chinese medicine composition is proved by experiments. The traditional Chinese medicine composition has the effects of strengthening spleen and tonifying qi, and can promote growth and improve production performance. Compared with antibiotics, hormone and chemical synthetic drugs, the compound preparation can prevent drug residues in vivo, has no toxic or side effect, is safe and reliable, and is suitable for livestock and poultry to promote growth.
The composition is prepared from the following raw materials in parts by weight: 3 to 20 parts of tuckahoe, 1 to 15 parts of astragalus, 1 to 20 parts of red sage root and 10 to 30 parts of broussonetia papyrifera.
The composition is prepared from the following raw materials in parts by weight: 5 to 15 parts of tuckahoe, 2 to 10 parts of astragalus, 4 to 15 parts of red sage root and 15 to 25 parts of broussonetia papyrifera.
The composition is prepared from the following raw materials in parts by weight: 8-12 parts of poria cocos, 4-8 parts of astragalus membranaceus, 6-10 parts of radix salviae miltiorrhizae and 20-25 parts of broussonetia papyrifera.
The composition is prepared from the following raw materials in parts by weight: 10 parts of poria cocos, 7 parts of astragalus membranaceus, 8 parts of radix salviae miltiorrhizae, and 25 parts of broussonetia papyrifera leaves.
The composition is prepared by weighing the components in parts by weight, respectively crushing, sieving, uniformly mixing, and preparing traditional Chinese medicine powder for animals.
The active ingredients of the poria cocos used for preparing the composition are carboxymethyl pachyman, and the carboxymethyl pachyman is prepared by the following method: pulverizing Poria, and sieving to obtain Poria powder; adding 10-30 times of 0.3-0.7 mol/L sodium hydroxide solution (equivalent to the mass of medicinal materials) under rapid stirring, alkalizing for 3-8 hours at 25-40 ℃, filtering or centrifuging to obtain alkalized solution; simultaneously adding 5 times of chloroacetic acid and sodium hydroxide solution (the molar ratio is 1:0.6-1.8) into the alkalization solution under rapid stirring, reacting for 2-4 hours at 40-80 ℃, then adjusting the pH to 13 by using the sodium hydroxide solution, and continuing to react for 2-8 hours; after the reaction is finished, regulating the pH to 10 by using a hydrochloric acid solution, ultrafiltering, carrying out alcohol precipitation on filtrate by using ethanol with the volume of 2-4 times, filtering or centrifuging, washing alcohol precipitate by using absolute ethanol, drying until the alcohol precipitate is complete, and crushing to obtain pachyman.
The astragalus and the red sage root used for preparing the composition are prepared according to the following method: reflux extracting radix astragali and radix Salviae Miltiorrhizae with 6-10 times (corresponding to the mass of the medicinal materials) of water for 1-3 times, each time for 0.5-2 hours, mixing the extractive solutions, concentrating under reduced pressure to obtain soft extract with relative density of 1.10-1.20 (55deg.C), precipitating with 2-4 times of ethanol, filtering or centrifuging, washing the alcohol precipitate with absolute ethanol, drying to completion, and pulverizing to obtain radix astragali and radix Ginseng polysaccharide.
The broussonetia papyrifera leaves used for preparing the composition are prepared according to the following method: reflux-extracting Broussonetia papyrifera leaves in an extraction tank with 10-15 times (corresponding to the mass of medicinal materials) of 80-95% ethanol for 1-3 hours, and discarding reflux liquid; reflux extracting the residue with 10-15 times (corresponding to the mass of the medicinal materials) of water for 1-3 times, each time for 1-2 hours, mixing the extracting solutions, concentrating under reduced pressure to obtain thick paste with the relative density of 1.10-1.20 (55 ℃), alcohol separating with 2-4 times of ethanol, filtering or centrifuging, washing the alcohol separating substance with absolute ethanol, drying to be complete, and pulverizing to obtain the paper mulberry polysaccharide.
The preparation process of the traditional Chinese medicine composition comprises the following steps: weighing the raw materials according to the weight parts, wherein poria cocos is prepared into carboxymethyl pachyman according to the method; astragalus and red sage are prepared into astragalus and red sage polysaccharide according to the method; preparing broussonetia papyrifera leaf polysaccharide by the method; mixing the three extracts, and making into oral liquid, powder or granule according to pharmaceutical method.
The traditional Chinese medicine composition has the effects of strengthening spleen and tonifying qi, can promote growth and improve production performance, and can be used in livestock and poultry animals.
Poria is sweet and light in taste, has effects of relieving nature, nourishing heart, lung, spleen and kidney channels, promoting diuresis, eliminating dampness, invigorating spleen, and calming heart; astragalus mongholicus is sweet in taste and slightly warm in nature, enters spleen and lung meridians, and has the effects of tonifying qi and raising yang, consolidating exterior and arresting sweating; the red sage root is bitter in taste and slightly cold in nature, enters heart and liver meridians, and has the effects of activating blood circulation to remove stasis, dredging meridians to relieve pain, cooling blood and eliminating carbuncles; the broussonetia papyrifera leaves are sweet and cool in taste and have the effects of clearing heat, cooling blood and promoting diuresis. The composition is prepared from the 4 traditional Chinese medicines, and the main active ingredients are plant polysaccharide, so that the immune barrier of an organism can be enhanced, the nutrient substances consumed by the organism for supporting immune response can be reduced, and the inhibition effect of response products in the immune response on feeding and growth can be reduced, thereby achieving the purpose of promoting the growth of animals.
The beneficial effects of the invention are as follows:
1. the traditional Chinese medicine composition is prepared from natural Chinese herbal medicines and has the advantages of long duration of action, no residue, small stimulation to animal organisms and the like.
2. The traditional Chinese medicine composition has the advantages of remarkable effect, mild effect, spleen strengthening and qi tonifying effects, growth promotion and production performance improvement, and is suitable for various livestock and poultry animals.
3. The traditional Chinese medicine composition can be prepared into oral liquid, granules and powder for drinking water or mixing materials according to the feeding habit of livestock and poultry.
4. The preparation method disclosed by the invention is simple in preparation process, convenient to use, easy to obtain raw materials, low in price and environment-friendly.
Detailed Description
EXAMPLE 1 preparation of powder
The preparation formula comprises the following steps: 50kg of poria cocos, 40kg of astragalus membranaceus, 50kg of radix salviae miltiorrhizae and 100kg of broussonetia papyrifera leaves.
The preparation method comprises the following steps: weighing the above medicinal materials according to the prescription, respectively pulverizing, sieving with 24 mesh sieve, and mixing.
EXAMPLE 2 preparation of powder
The preparation formula comprises the following steps: 100kg of poria cocos, 70kg of astragalus membranaceus, 80kg of radix salviae miltiorrhizae and 250kg of broussonetia papyrifera.
Preparing raw materials:
(1) Pulverizing 100kg of Poria to obtain Poria powder; adding 2400L of 0.7mol/L sodium hydroxide solution under rapid stirring, alkalizing at 25 ℃ for 8 hours, and filtering to obtain alkalized solution; adding 500L of 3.6mol/L chloroacetic acid and 2.16mol/L sodium hydroxide solution into the alkalization solution under rapid stirring, reacting for 2 hours at 60 ℃, then adjusting the pH to 13 by using the sodium hydroxide solution, and continuing the reaction for 5 hours; after the reaction, the pH value is adjusted to 10 by using 2mol/L hydrochloric acid solution, the mixture passes through an ultrafiltration membrane (the molecular weight is 7000 daltons), the filtrate is subjected to alcohol precipitation by using ethanol with the volume of 2 times, the mixture is centrifuged, and the alcohol precipitate is washed by using absolute ethanol, dried and crushed to obtain 66.8kg of carboxymethyl pachyman.
(2) Extracting 70kg of radix astragali and 80kg of radix Salviae Miltiorrhizae with 900L water under reflux for 3 times (each for 0.5 hr), mixing extractive solutions, concentrating under reduced pressure to obtain soft extract with relative density of 1.20 (55deg.C), precipitating with 3 times of ethanol, centrifuging, washing the precipitate with anhydrous ethanol, drying, and pulverizing to obtain 31.6kg of radix astragali and radix Ginseng polysaccharide.
(3) Placing 250kg of broussonetia papyrifera leaves in an extraction tank, reflux-extracting with 2500L of 80% ethanol for 2 hours, and discarding reflux liquid; reflux extracting the residue with 3000L water for 1 time and 2 hr each time, mixing the extractive solutions, concentrating under reduced pressure to obtain soft extract with relative density of 1.15 (55deg.C), precipitating with 2 times ethanol, centrifuging, washing the alcohol precipitate with anhydrous ethanol, drying, and pulverizing to obtain 61.2kg of Broussonetia papyrifera polysaccharide.
(4) Preparing: mixing the polysaccharide extract with 90.4kg glucose, and packaging.
Example 3 preparation of oral liquid
The preparation formula comprises the following steps: 60kg of poria cocos, 160kg of astragalus membranaceus, 200kg of radix salviae miltiorrhizae and 600kg of broussonetia papyrifera leaves.
Preparing raw materials:
(1) Pulverizing 60kg of Poria to obtain Poria powder; adding 600L of 0.5mol/L sodium hydroxide solution under rapid stirring, alkalizing at 40 ℃ for 5 hours, and filtering to obtain alkalized solution; adding 300L of 3.15mol/L chloroacetic acid and 5.67mol/L sodium hydroxide solution into the alkalization solution under rapid stirring, reacting for 4 hours at 40 ℃, then adjusting the pH to 13 by using the sodium hydroxide solution, and continuing reacting for 2 hours; after the reaction, the pH was adjusted to 10 with a 2mol/L hydrochloric acid solution, and the mixture was passed through an ultrafiltration membrane (molecular weight: 7000 daltons), and the filtrate was subjected to alcohol precipitation with 4 volumes of ethanol, centrifugation, washing the alcohol precipitate with absolute ethanol, drying, and pulverizing to obtain 31.7kg of pachymaran.
(2) Putting 160kg of astragalus and 200kg of red sage root into the same extraction tank, reflux-extracting with 2160L of water for 2 times each for 2 hours, combining the extracting solutions, concentrating under reduced pressure to obtain thick paste with the relative density of 1.15 (55 ℃), alcohol-separating with 2 times of ethanol, centrifuging, washing the alcohol-separating substance with absolute ethanol, drying and pulverizing to obtain 65.6kg of astragalus and ginseng polysaccharide.
(3) Placing 600kg of broussonetia papyrifera leaves in an extraction tank, reflux-extracting with 6000L of 90% ethanol for 3 hours, and discarding reflux liquid; reflux extracting the residue with 6000L water for 2 times each for 1 hr, mixing extractive solutions, concentrating under reduced pressure to obtain soft extract with relative density of 1.20 (55deg.C), precipitating with 3 times ethanol, centrifuging, washing the alcohol precipitate with anhydrous ethanol, drying, and pulverizing to obtain Broussonetia papyrifera polysaccharide 145.2kg.
(4) Placing the above 3 polysaccharide extracts into a preparation tank, adding 1800L of water, stirring for dissolving, refrigerating for 24 hr, filtering with pulp board, adding 3%o sodium benzoate and potassium sorbate, stirring for dissolving, adding water to 2000L, mixing, sterilizing with circulating steam for 30 min, and packaging.
Example 4 preparation of oral liquid
The preparation formula comprises the following steps: 200kg of poria cocos, 200kg of astragalus membranaceus, 300kg of radix salviae miltiorrhizae and 300kg of broussonetia papyrifera leaves.
Preparing raw materials:
(1) Pulverizing 200kg of Poria to obtain Poria powder; adding 3000L of 0.45mol/L sodium hydroxide solution under rapid stirring, alkalizing at 35 ℃ for 5.5 hours, and filtering to obtain alkalized solution; 1000L of 3.02mol/L chloroacetic acid and 4.53mol/L sodium hydroxide solution are added into the alkalization solution under rapid stirring, the reaction is carried out for 3.5 hours at 50 ℃, then the pH is regulated to 13 by the sodium hydroxide solution, and the reaction is continued for 2.5 hours; after the reaction, the pH was adjusted to 10 with a 2mol/L hydrochloric acid solution, and the mixture was passed through an ultrafiltration membrane (molecular weight: 7000 daltons), and the filtrate was subjected to alcohol precipitation with 4 volumes of ethanol, centrifugation, washing the alcohol precipitate with absolute ethanol, drying, and pulverizing to obtain 117.3kg of pachymaran.
(2) 200kg of astragalus and 300kg of red sage root are taken and put into a same extraction tank, reflux-extracted for 3 times with 4500L of water for 1.5 hours each time, the extracting solutions are combined, decompressed and concentrated to thick paste with the relative density of 1.15 (55 ℃), alcohol precipitation is carried out by using 2 times of ethanol, centrifugation, alcohol precipitation is washed by using absolute ethanol, and drying and crushing are carried out, thus obtaining 108.6kg of astragalus and ginseng polysaccharide.
(3) Extracting 300kg of broussonetia papyrifera leaves in an extraction tank with 4500L of 85% ethanol under reflux for 2.5 hours, and discarding the reflux liquid; reflux extracting the residue with 3600L water for 3 times each for 2 hr, mixing extractive solutions, concentrating under reduced pressure to obtain soft extract with relative density of 1.20 (55deg.C), precipitating with 3 times ethanol, centrifuging, washing the alcohol precipitate with anhydrous ethanol, drying, and pulverizing to obtain 76.4kg of Broussonetia papyrifera polysaccharide.
(4) Placing the above 3 polysaccharide extracts into a preparation tank, adding 1800L of water, stirring for dissolving, refrigerating for 24 hr, filtering with pulp board, adding 3%o sodium benzoate and potassium sorbate, stirring for dissolving, adding water to 2000L, mixing, sterilizing with circulating steam for 30 min, and packaging.
Example 5 preparation of granules
The preparation formula comprises the following steps: 200kg of poria cocos, 10kg of astragalus membranaceus, 10kg of radix salviae miltiorrhizae and 200kg of broussonetia papyrifera leaves.
Preparing raw materials:
(1) Pulverizing 200kg of Poria to obtain Poria powder; adding 6000L of 0.3mol/L sodium hydroxide solution under rapid stirring, alkalizing for 3 hours at 30 ℃, and filtering to obtain alkalized solution; 1000L of 3.35mol/L chloroacetic acid and 3.96mol/L sodium hydroxide solution are added into the alkalization liquid under rapid stirring, the reaction is carried out for 3 hours at 80 ℃, then the pH is regulated to 13 by the sodium hydroxide solution, and the reaction is continued for 8 hours; after the reaction, the pH value is adjusted to 10 by using 2mol/L hydrochloric acid solution, the mixture passes through an ultrafiltration membrane (the molecular weight is 7000 daltons), the filtrate is subjected to alcohol precipitation by using 3 times of volume of ethanol, the alcohol precipitation is centrifuged, and the alcohol precipitation is washed by using absolute ethanol, dried and crushed to obtain 125.9kg of carboxymethyl pachyman.
(2) Reflux-extracting radix astragali 10kg and Saviae Miltiorrhizae radix 10kg with 200L water for 1 hr each time in the same extraction tank, mixing extractive solutions, concentrating under reduced pressure to obtain soft extract with relative density of 1.10 (55deg.C), precipitating with 4 times of ethanol, centrifuging, washing the alcohol precipitate with anhydrous ethanol, drying, and pulverizing to obtain radix astragali and Ginseng radix polysaccharide 3.7kg.
(3) Placing 200kg of broussonetia papyrifera leaves in an extraction tank, reflux-extracting with 2400L of 95% ethanol for 1 hr, and discarding reflux liquid; reflux extracting the residue with 3000L water for 3 times each for 1.5 hr, mixing extractive solutions, concentrating under reduced pressure to obtain soft extract with relative density of 1.10 (55deg.C), precipitating with 4 times ethanol, centrifuging, washing the alcohol precipitate with anhydrous ethanol, drying, and pulverizing to obtain Broussonetia papyrifera polysaccharide 57.9kg.
(4) Mixing the above 3 polysaccharide extracts with dextrin 150kg and sugar powder 83.4kg, granulating with 60% ethanol as binder, drying, grading, and packaging.
Example 6 preparation of granules
The preparation formula comprises the following steps: 50kg of poria cocos, 60kg of astragalus mongholicus, 90kg of radix salviae miltiorrhizae and 250kg of broussonetia papyrifera leaves.
Preparing raw materials:
(1) Pulverizing 50kg of Poria to obtain Poria powder; adding 600L of 0.35mol/L sodium hydroxide solution under rapid stirring, alkalizing at 35 ℃ for 6.5 hours, and filtering to obtain alkalized solution; adding 250L of 3.58mol/L chloroacetic acid and 3.58mol/L sodium hydroxide solution into the alkalization solution under rapid stirring, reacting for 2.5 hours at 70 ℃, then adjusting the pH to 13 by using the sodium hydroxide solution, and continuing the reaction for 6.5 hours; after the reaction, the pH value is adjusted to 10 by using 2mol/L hydrochloric acid solution, the mixture passes through an ultrafiltration membrane (the molecular weight is 7000 daltons), the filtrate is subjected to alcohol precipitation by using 3 times of volume of ethanol, the alcohol precipitation is centrifuged, and the alcohol precipitation is washed by using absolute ethanol, dried and crushed to obtain 29.9kg of carboxymethyl pachyman.
(2) Reflux-extracting radix astragali 60kg and Saviae Miltiorrhizae radix 90kg with 1500L water for 2 times each for 1.5 hr, mixing extractive solutions, concentrating under reduced pressure to obtain soft extract with relative density of 1.10 (55deg.C), precipitating with 3 times of ethanol, centrifuging, washing the alcohol precipitate with anhydrous ethanol, drying, and pulverizing to obtain radix astragali and Ginseng radix polysaccharide 28.7kg.
(3) Placing 250kg of broussonetia papyrifera leaves in an extraction tank, reflux-extracting with 2500L of 90% ethanol for 2 hours, and discarding reflux liquid; reflux extracting the residue with 3000L water for 2 times each for 1.5 hr, mixing extractive solutions, concentrating under reduced pressure to obtain soft extract with relative density of 1.10 (55deg.C), precipitating with 3 times ethanol, centrifuging, washing the alcohol precipitate with anhydrous ethanol, drying, and pulverizing to obtain Broussonetia papyrifera polysaccharide 64.6kg.
(4) Mixing the above 3 polysaccharide extracts with dextrin 220kg and sugar powder 106.8kg, granulating with 60% ethanol as binder, drying, grading, and packaging.
EXAMPLE 7 preparation of powder
The preparation formula comprises the following steps: 200kg of poria cocos, 140kg of astragalus and 160kg of radix salviae miltiorrhizae.
Preparing raw materials:
(1) Pulverizing 200kg of Poria to obtain Poria powder; adding 0.7mol/L sodium hydroxide solution 4800L under rapid stirring, alkalizing at 25deg.C for 8 hr, and filtering to obtain alkalized solution; 1000L of 3.6mol/L chloroacetic acid and 2.16mol/L sodium hydroxide solution are added into the alkalization liquid under rapid stirring, the reaction is carried out for 2 hours at 60 ℃, then the pH is regulated to 13 by the sodium hydroxide solution, and the reaction is continued for 5 hours; after the reaction, the pH value is adjusted to 10 by using 2mol/L hydrochloric acid solution, the mixture passes through an ultrafiltration membrane (the molecular weight is 7000 daltons), the filtrate is subjected to alcohol precipitation by using ethanol with the volume of 2 times, the mixture is centrifuged, and alcohol precipitation is washed by using absolute ethanol, dried and crushed to obtain 136.3kg of carboxymethyl pachyman.
(2) 140kg of astragalus and 160kg of red sage root are taken and put into the same extraction tank, reflux-extracted with 1800L of water for 3 times, each time for 0.5 hour, the extracting solutions are combined, decompressed and concentrated to thick paste with the relative density of 1.20 (55 ℃), alcohol precipitation is carried out by 3 times of ethanol, centrifugation, the alcohol precipitation is washed by absolute ethanol, and the dried and crushed astragalus and ginseng polysaccharide 62.3kg is obtained.
(3) Preparing: mixing the polysaccharide extract with 51.4kg glucose, and packaging.
Example 8 preparation of comparative drug
Refer to patent CN106913743a: a preparation method of veterinary drug for promoting animal growth is provided.
Mixing radix Codonopsis 25kg, radix astragali 15kg, poria 15kg, atractylodis rhizoma 10kg, pericarpium Citri Tangerinae 10kg, rhizoma Dioscoreae 10kg, and brown sugar 15kg, pulverizing into 50 mesh powder, sieving, packaging, and storing.
Example 9 clinical effect test on growth promotion of broiler chickens
The test method comprises the following steps: 1 day old broiler 810 was randomly divided into 9 groups of 3 replicates each, 30 replicates each. The blank control group was fed with basal diet, and the rest of test groups and the comparison group were added with test samples as required in table 1 for 42 consecutive days. Grouping and dosing are shown in table 1.
Table 1 test group and dosing conditions
| Group of | Test article | Dosage of usage | Equivalent to the crude drug amount | Period of use (Tian) | Animal number (only) |
| Blank group | — | — | — | — | 90 |
| Test 1 group | Example 1 | 0.2% of mixing material | 2kg crude drug/t | 42 | 90 |
| Test 2 groups | Example 2 | 0.05% drinking water | 2kg crude drug/t | 42 | 90 |
| Test 3 groups | Example 3 | 0.2% drinking water | 2kg crude drug/t | 42 | 90 |
| Test 4 groups | Example 4 | 0.2% drinking water | 2kg crude drug/t | 42 | 90 |
| Test 5 groups | Example 5 | 0.1% drinking water | 2kg crude drug/t | 42 | 90 |
| Test 6 groups | Example 6 | 0.1% drinking water | 2kg crude drug/t | 42 | 90 |
| Test 7 groups | Example 7 | 0.05% drinking water | 2kg crude drug/t | 42 | 90 |
| Comparison group | Example 8 | 1% of mixing material | 10kg crude drug/t | 42 | 90 |
Test results (as in table 2): (1) compared with a blank control group, the test group and the comparison group can increase the slaughter weight of the broiler chickens, improve the survival rate and reduce the feed conversion ratio. (2) The test group has advantages over the control group in increasing the weight of the broiler in the slaughter house, improving the survival rate, reducing the feed conversion ratio and the like. (3) Among the test groups, the test 2 group, the test 4 group and the test 6 group had remarkable effects, and the test 2 group (optimum ratio) had the best effect. The product is suggested to improve the growth performance of chickens when coming out.
Table 2 table of statistics of results of broiler tests
| Group of | Go out of the fence body weight (kg) | Survival (%) | Feed to meat ratio |
| Blank control group | 2.663±0.221 | 94.76 | 1.765 |
| Test 1 group | 2.721±0.208 | 95.81 | 1.734 |
| Test 2 groups | 2.812±0.192* | 98.02 | 1.651 |
| Test 3 groups | 2.735±0.215 | 96.27 | 1.713 |
| Test 4 groups | 2.771±0.199* | 97.48 | 1.682 |
| Test 5 groups | 2.762±0.211 | 96.87 | 1.691 |
| Test 6 groups | 2.799±0.187* | 97.86 | 1.677 |
| Test 7 groups | 2.720±0.217 | 95.65 | 1.739 |
| Comparison group | 2.717±0.238 | 95.44 | 1.748 |
Note that: * Indicating P <0.05 compared to the blank.
Example 10 clinical effect test on growth-promoting of weaned piglets
The test method comprises the following steps: healthy weaned pigs 162 with similar gestation, day-old and weight are selected and randomly divided into 9 groups of 3 replicates each and 6 replicates each. The blank control group was fed with basal diet, and the rest of test groups and the comparison group were added with test samples as required in table 3 for 35 consecutive days. Grouping and dosing are shown in table 3.
Table 3 test group and dosing conditions
| Group of | Test article | Dosage of usage | Equivalent to the crude drug amount | Period of use (Tian) | Animal number (only) |
| Blank group | — | — | — | 18 | |
| Test 1 group | Example 1 | 0.2% of mixing material | 2kg crude drug/t | 35 | 18 |
| Test 2 groups | Example 2 | 0.1% of mixing material | 2kg crude drug/t | 35 | 18 |
| Test 3 groups | Example 3 | 0.4% of mixing material | 2kg crude drug/t | 35 | 18 |
| Test 4 groups | Example 4 | 0.4% of mixing material | 2kg crude drug/t | 35 | 18 |
| Test 5 groups | Example 5 | 0.2% of mixing material | 2kg crude drug/t | 35 | 18 |
| Test 6 groups | Example 6 | 0.2% of mixing material | 2kg crude drug/t | 35 | 18 |
| Test 7 groups | Example 7 | 0.1% of mixing material | 2kg crude drug/t | 35 | 18 |
| Comparison group | Example 8 | 1% of mixing material | 10kg crude drug/t | 35 | 18 |
Test results (as in table 4): (1) compared with a blank control group, the test group and the comparison group can increase the final weight and daily gain of piglets and reduce the feed conversion ratio. (2) The test group increased the final weight and daily gain, reduced the feed conversion ratio, etc., and was superior to the control group. (3) Among the test groups, the test 2 group, the test 4 group and the test 6 group had remarkable effects, and the test 2 group (optimum ratio) had the best effect. The product can improve the growth performance of piglets.
Table 4 statistics of weaned pig test results
| Group of | Initial weight (kg) | Final body weight (kg) | Daily gain (kg) | Daily feed intake (kg) | Feed to meat ratio |
| Blank control group | 7.22±0.68 | 23.41±1.01 | 0.46±0.02 | 0.865 | 1.87 |
| Test 1 group | 7.19±0.69 | 23.96±1.04 | 0.48±0.03 | 0.872 | 1.82 |
| Test 2 groups | 7.20±0.67 | 26.02±1.25* | 0.54±0.04* | 0.930 | 1.73 |
| Test 3 groups | 7.21±0.62 | 24.34±1.22 | 0.49±0.03 | 0.881 | 1.80 |
| Test 4 groups | 7.19±0.63 | 25.41±1.15 | 0.52±0.02* | 0.922 | 1.77 |
| Test 5 groups | 7.18±0.65 | 24.67±1.09 | 0.50±0.04 | 0.896 | 1.79 |
| Test 6 groups | 7.21±0.52 | 25.88±1.11* | 0.53±0.05* | 0.938 | 1.76 |
| Test 7 groups | 7.19±0.66 | 23.78±1.04 | 0.47±0.03 | 0.861 | 1.83 |
| Comparison group | 7.22±0.56 | 24.05±1.18 | 0.48±0.05 | 0.883 | 1.84 |
Note that: * Indicating P <0.05 compared to the blank.
While specific embodiments of the invention have been described above, it will be appreciated by those skilled in the art that the specific embodiments described are illustrative only and not intended to limit the scope of the invention, and that equivalents and modifications, in accordance with the spirit of the invention, will be within the scope of the invention as defined by the appended claims.
Claims (3)
1. A traditional Chinese medicine composition for promoting growth is characterized in that: the composition is prepared from the following raw materials in parts by weight: 8-12 parts of poria cocos, 4-8 parts of astragalus membranaceus, 6-10 parts of radix salviae miltiorrhizae and 20-25 parts of broussonetia papyrifera leaves; the active ingredients of the poria cocos used for preparing the composition are carboxymethyl pachyman, and the carboxymethyl pachyman is prepared by the following method: pulverizing Poria, and sieving to obtain Poria powder; adding 10-30 times of 0.3-0.7 mol/L sodium hydroxide solution under rapid stirring, alkalizing for 3-8 hours at 25-40 ℃, and filtering or centrifuging to obtain alkalized solution; simultaneously adding chloroacetic acid and sodium hydroxide solution with the molar ratio of 1:0.6-1.8, which are 5 times of the mass of the medicinal materials, into the alkalization solution under the condition of rapid stirring, reacting for 2-4 hours at 40-80 ℃, then adjusting the pH to 13 by using the sodium hydroxide solution, and continuing the reaction for 2-8 hours; after the reaction is finished, regulating the pH to 10 by using a hydrochloric acid solution, ultrafiltering, carrying out alcohol precipitation on filtrate by using ethanol with the volume of 2-4 times, filtering or centrifuging, washing alcohol precipitate by using absolute ethanol, drying until the alcohol precipitate is complete, and crushing to obtain pachyman;
the effective components of astragalus and red sage root used for preparing the composition are astragalus and red sage root polysaccharide, and the composition is prepared by the following method: reflux extracting radix astragali and radix salviae miltiorrhizae with water with the quality of 6-10 times of the medicine quality for 1-3 times and each time for 0.5-2 hours, mixing the extracting solutions, concentrating under reduced pressure to thick paste with the relative density of 1.10-1.20 at 55 ℃, carrying out alcohol precipitation with 2-4 times of ethanol, filtering or centrifuging, washing alcohol precipitate with absolute ethanol, drying to be complete, and crushing to obtain astragalus-ginseng polysaccharide;
the active ingredients of the broussonetia papyrifera leaf used for preparing the composition are broussonetia papyrifera polysaccharide, and the broussonetia papyrifera leaf composition is prepared according to the following method: placing the broussonetia papyrifera leaves in an extraction tank, reflux-extracting with 80% -95% ethanol with the quality of the medicine material being 10-15 times and the volume fraction for 1-3 hours, and discarding the reflux liquid; reflux-extracting the residues with 10-15 times of water for 1-3 times, each time for 1-2 hours, mixing the extracting solutions, concentrating under reduced pressure to obtain thick paste with the relative density of 1.10-1.20 at 55 ℃, carrying out alcohol precipitation with 2-4 times of ethanol, filtering or centrifuging, washing the alcohol precipitate with absolute ethanol, drying to be complete, and pulverizing to obtain the paper mulberry polysaccharide.
2. The growth-promoting Chinese medicinal composition of claim 1, wherein: the preparation process of the traditional Chinese medicine composition comprises the following steps: weighing the raw materials according to the weight portion ratio, wherein poria cocos is used for preparing carboxymethyl pachyman according to the method; astragalus and red sage root are used for preparing astragalus and red sage root polysaccharide according to the method; preparing broussonetia papyrifera leaf polysaccharide by broussonetia papyrifera leaves according to the method; mixing the three extracts, and making into oral liquid, powder or granule according to pharmaceutical method.
3. The growth-promoting Chinese medicinal composition of claim 1, wherein: the traditional Chinese medicine composition has the effects of strengthening spleen and tonifying qi, promotes growth, improves production performance, and is used in livestock and poultry animals.
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| CN101664498A (en) * | 2009-09-29 | 2010-03-10 | 北京大北农动物保健科技有限责任公司 | Composite traditional Chinese medicine composition and preparation method and application thereof |
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| CN109985148A (en) * | 2017-12-29 | 2019-07-09 | 瑞普（天津）生物药业有限公司 | A kind of Chinese medicine composition of enhancing development |
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| CN106561982A (en) * | 2016-08-30 | 2017-04-19 | 南昌大学 | Livestock and poultry immunopotentiation feed additive prepared from broussonetia papyrifera stem leaf meal and microalgae powder and preparation method thereof |
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