CN114259531B - Extraction process and application of scindapsus aureus - Google Patents
Extraction process and application of scindapsus aureus Download PDFInfo
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Abstract
The invention discloses an extraction process of scindapsus aureus, which comprises the following steps: preparing green bonnie flower into powder, extracting for 1-2.5 hours by adopting 70-85% ethanol solution according to the material-liquid ratio of 1:10-15, standing, filtering, collecting alcohol precipitation solution, mixing the alcohol precipitation solution with the first filtrate, and distilling to obtain the extract. The method can effectively improve the extraction effect of the active ingredients of the green bonnie flower, so that the effects of extracting the coumarin and other ingredients by hot water extraction and then alcohol extraction are good, and a good blood pressure lowering effect is obtained.
Description
Technical Field
The invention relates to the technical field of scindapsus aureus flower extraction. More specifically, the invention relates to an extraction process and application of scindapsus aureus.
Background
The scindapsus aureus is a Tibetan medicine, is produced in cold regions of Tibet, is slightly cold in nature, has a growth period of more than 40 days, is a unique precious herb in Tibet, can grow only in Tibet, and is the picking season of the scindapsus aureus at the end of 10 months every year. The green bonnie flower has various effects, is used as a medicinal material in a prescription, increases the drug effect of a medicament, treats diseases, and is also often used as a mate in tea soup.
The medicinal components of the green bonnie flower are obtained by decocting, soaking and other modes, the utilization rate is low, and the exertion of the medicinal active components of the green bonnie flower is influenced.
Although the prior art (for example, publication No. CN 105310918B, entitled "a scindapsus aureus extract and its application) describes a method for ultrasonic extraction of scindapsus aureus by ethanol, the dissolution effect of the active ingredients of scindapsus aureus in organic solvents is different, and the extraction effect of scindapsus aureus is affected by the ethanol extraction of the prior art.
Disclosure of Invention
It is an object of the present invention to address at least the above problems and to provide at least the advantages described hereinafter.
The invention provides an extraction process of scindapsus aureus, wherein the leaching of coumarin and other components is promoted by passing the filter residue after alcohol extraction through a hot water body, so that the extraction effect of active ingredients of scindapsus aureus can be effectively improved, the effect of extracting coumarin and other components through hot water extraction and then alcohol extraction is good, and a good blood pressure lowering effect is obtained.
To achieve these objects and other advantages in accordance with the present invention, there is provided a process for extracting scindapsus aureus, which comprises powdering scindapsus aureus, extracting with 70-85% ethanol solution at a feed-to-liquid ratio of 1-15 g/ml for 1-2.5 hours, filtering the ethanol extract to obtain a first filtrate, extracting the residue with hot water, filtering the water extract to obtain a second filtrate, mixing the second filtrate with 90-95% ethanol at a volume ratio of 1:10-15, standing, filtering, collecting alcohol precipitation solution, mixing the alcohol precipitation solution with the first filtrate, and distilling to obtain the extract. References herein to% ethanol concentration are generally to volume fraction.
Preferably, the temperature of the hot water is 70-100 ℃, the water extraction frequency of the filter residue is 2 times, and the mass ratio of the filter residue obtained by the first water extraction to the water is 1:5, the mass ratio of filter residue obtained by secondary water extraction to water is 1:10, the water extraction time is 1-2 hours each time.
Preferably, the standing time is 5 to 10 hours.
Preferably, the alcohol extraction times of the first filtrate is 2, the first alcohol extraction time is 1.5 hours, and the second alcohol extraction time is 1 hour.
Preferably, the scindapsus aureus flower powder is prepared by the following method: and (3) carrying out vacuum freeze drying on the cleaned scindapsus aureus in a dark environment, and crushing the dried scindapsus aureus in a vacuum environment to obtain scindapsus aureus powder.
Preferably, the vacuum freeze drying has freezing time of 1.5 hr, freezing temperature of-20 deg.C, sublimation drying time of 2 hr, sublimation drying temperature of 45 deg.C, vacuum degree of-0.01 Mpa, and pulverized particle size of 35 mesh.
Preferably, the scindapsus aureus flower powder is subjected to alcohol extraction, and the method further comprises the following steps: placing the green bonnie pollen powder in a non-metal closed container, introducing negative hydrogen ion gas into the closed container, wherein the concentration of the hydrogen ion gas in the closed container is 6 multiplied by 10 5 Per cm 3 The pressure is 0.1Mpa, and the nonmetal stirring rod in the closed container is rotated for 1-2 hours.
Preferably, negative hydrogen ion gas is circularly introduced into the alcohol precipitation solution when the alcohol precipitation solution and the first filtrate are combined and distilled, and the concentration of the negative hydrogen ion gas is 6 multiplied by 10 5 Per cm 3 Negative hydrogen ion gas is introduced from the bottom of the distillation flask and is introduced into the gas pipe at the bottom of the distillation flaskA plurality of air outlets are uniformly distributed on the distillation flask and used for allowing negative hydrogen ion gas to flow into the solution from the air outlets, so that the negative hydrogen ion gas is mixed in the solution, and then the emitted gas is pumped out from the top of the distillation flask to form circulation.
The invention also provides application of the extract of the green bonnie flower extracted by the process in preparing a medicine for reducing blood pressure.
The invention at least comprises the following beneficial effects:
firstly, the filter residue after alcohol extraction is subjected to hot water extraction, so that the dissolution of components such as coumarins is promoted, the extraction effect of the active components of the scindapsus aureus can be effectively improved, the effects of extracting the components such as the coumarins through hot water extraction and then alcohol extraction are good, and a good blood pressure lowering effect is obtained.
Secondly, the invention carries out vacuum freeze drying in a dark environment, reduces the oxidation and volatilization of components such as coumarins, flavonoids and the like in the scindapsus aureus, and is beneficial to improving the extraction effect and activity of the extract.
Fourthly, the scindapsus aureus flower powder is mixed with negative hydrogen ion gas in advance through alcohol, and partially oxidized coumarins and flavonoids compounds in the crushing process are reduced, so that the extraction effect and the activity of the extract are improved.
Fifthly, the invention can improve the distillation efficiency and reduce partially oxidized coumarins and flavonoids compounds in the extraction process by circularly introducing the negative hydrogen ion gas into the combined distillation solution, thereby further improving the extraction effect and activity of the extract.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
It is to be noted that the experimental methods described in the following embodiments are all conventional methods unless otherwise specified, and the reagents and materials are commercially available unless otherwise specified.
< example 1>
An extraction process of scindapsus aureus, which comprises the following steps:
step one, putting the cleaned green bonnie flower in a vacuum freeze dryer in a dark environment, carrying out vacuum freezing for 1.5h at the temperature of minus 20 ℃, carrying out sublimation drying for 2h at the temperature of 45 ℃, wherein the vacuum degree in the whole process is minus 0.01Mpa, and then crushing and sieving by a 35-mesh sieve to obtain green bonnie flower powder;
step two, carrying out alcohol extraction on the green bonnie flower powder and an ethanol solution with the concentration of 75% for 2 times under the normal temperature and pressure environment, wherein the material-liquid ratio of primary alcohol extraction is 1;
and step three, carrying out water extraction on the filter residue by using hot water at the temperature of 80 ℃, wherein the water extraction frequency is 2 times, and the mass ratio of the filter residue subjected to the first water extraction to the water is 1:5, the mass ratio of filter residue obtained by secondary water extraction to water is 1:10, each water extraction time is 1 hour; and mixing the two water extract filtrates to obtain a second filtrate, wherein the volume ratio of the second filtrate to 95% ethanol is 1:12, standing the mixed solution for 8 hours, filtering and collecting an alcohol precipitation solution, mixing the alcohol precipitation solution with the first filtrate, and distilling to obtain the extract.
The animal experiments for the hypotensive effect of the extract of example 1 were as follows:
1. specification and feeding requirements of laboratory rats
The male SD rat is 36 healthy male SD rats with SPF grade and weight (175 +/-30) g, all experimental animals are raised in a controllable environment at room temperature of 18-24 ℃ and humidity of 40-50%, the animals eat and drink water freely during the experiment, and the circadian rhythm is normal.
2. Experimental methods
(1) Establishing hypertension rat model
The healthy male SD rats are fed for 1 week adaptively, the blood pressure of the tail artery of each rat is measured by an ALC-NIBP noninvasive blood pressure determination and analysis system before model building, the measurement is repeated for 3 times, and the average value is taken and recorded. A hypertension rat model is prepared by adopting a 'two kidneys and one clip' method. After 4 weeks of molding, 30 rats meet the Systolic Pressure (SP) which is more than 120mmHg, and the blood pressure after molding is more than 20mmHg higher than that before molding, namely the experimental hypertension rats.
(2) Experimental grouping and administration mode
Taking 30 hypertension rats successfully modeled, randomly dividing the hypertension rats into 5 groups, wherein each group comprises 6 rats, namely a model control group, a positive drug control group (captopril group), an extract high dose group, an extract medium dose group and an extract low dose group; another 6 healthy male SD rats were used as a blank control group. After the model is successfully established for 4 weeks, after the SP of the rat is stabilized, the blood pressure of the tail artery of the rat in the waking state is determined by an ALC-NIBP noninvasive blood pressure determination analysis system before administration. The high, medium and low dose groups of the extract are respectively 5ml/kg, 10ml/kg and 15ml/kg, the administration is performed by intragastric administration, the dose of the captopril group is 7.0mg/kg, the administration is performed by intragastric administration, the rats of the model control group and the blank control group are intragastric administered with physiological saline with the same amount as the rats every day, the administration is performed for 1 time every day beginning at the 5 th week, and the administration is performed continuously for 4 weeks. Wherein, the dosage of the equivalent normal saline is the same as that of the abdominal cavity; in the experiment, 7.0mg/kg of captopril is selected as the optimal administration dose screened in the experiment.
3. Blood pressure measurement
Before the model building, at the 4 th week of the model building and at the 4 th week of the drug administration, the caudal artery Systolic Pressure (SP) of the rat in the waking state is respectively measured by an ALC-NIBP non-invasive blood pressure measurement and analysis system, the measurement is repeated for 3 times, the average value is taken, and the obtained data is detailed in a table 1.
Table 1: rat blood pressure changes (
mmHg)
Note: the self blood pressure before and after the administration is compared, △ P<0.05， △△ P<0.01; compared with the model control group, * P<0.05， ** P<0.01。
the data in Table 1 show that the extract is effective in lowering blood pressure in hypertensive rats. The blood pressure of the hypertension rats successfully modeled reaches the hypertension index. After 4 weeks of administration, the high, medium and low dose groups of the extract and the captopril group all produced obvious blood pressure lowering effects, and compared with a model control group, the high, medium and low dose groups of the extract in example 1 (5 ml/kg, 10ml/kg and 15 ml/kg) all produced obvious blood pressure lowering effects compared with the self blood pressure before and after administration (P < 0.01). The data in table 1 clearly show that the extract has a better blood pressure lowering effect when applied to the medicine for treating hypertension.
< example 2>
An extraction process of scindapsus aureus, which comprises the following steps:
step one, putting the cleaned green bonnie flower in a vacuum freeze dryer in a dark environment, carrying out vacuum freezing for 1.5h at the temperature of minus 20 ℃, carrying out sublimation drying for 2h at the temperature of 45 ℃, wherein the vacuum degree in the whole process is minus 0.01Mpa, and then crushing and sieving by a 35-mesh sieve to obtain green bonnie flower powder;
secondly, carrying out alcohol extraction on the green bonnie flower powder and an ethanol solution with the concentration of 70% for 2 times under the normal temperature and pressure environment, wherein the material-liquid ratio of primary alcohol extraction is 1;
and step three, carrying out water extraction on the filter residue by using hot water at the temperature of 70 ℃, wherein the water extraction frequency is 2 times, and the mass ratio of the filter residue subjected to the first water extraction to the water is 1:5, the mass ratio of filter residue obtained by secondary water extraction to water is 1:10, each water extraction time is 1 hour; and (3) mixing the two water extract filtrates to obtain a second filtrate, wherein the volume ratio of the second filtrate to 90% ethanol is 1:10, standing the mixed solution for 5 hours, filtering and collecting an alcohol precipitation solution, combining the alcohol precipitation solution and the first filtrate, and distilling to obtain the extract.
< example 3>
An extraction process of scindapsus aureus, which comprises the following steps:
step one, putting the cleaned green bonnie flower in a vacuum freeze dryer in a dark environment, carrying out vacuum freezing for 1.5h at the temperature of minus 20 ℃, carrying out sublimation drying for 2h at the temperature of 45 ℃, wherein the vacuum degree in the whole process is minus 0.01Mpa, and then crushing and sieving by a 35-mesh sieve to obtain green bonnie flower powder;
step two, carrying out alcohol extraction on the green bonnie flower powder and an ethanol solution with the concentration of 85% for 2 times under the normal temperature and pressure environment, wherein the material-liquid ratio of primary alcohol extraction is 1;
and step three, performing water extraction on the filter residue by using hot water at the temperature of 100 ℃, wherein the water extraction frequency is 2 times, and the mass ratio of the filter residue obtained by the first water extraction to the water is 1:5, the mass ratio of filter residue obtained by secondary water extraction to water is 1:10, the water extraction time is 1 hour each time; and (3) mixing the two water extract filtrates to obtain a second filtrate, wherein the volume ratio of the second filtrate to 95% ethanol is 1:15, standing the mixed solution for 10 hours, filtering and collecting an alcohol precipitation solution, combining the alcohol precipitation solution and the first filtrate, and distilling to obtain the extract.
< example 4>
An extraction process of scindapsus aureus, which comprises the following steps:
step one, putting the cleaned green bonnie flower in a vacuum freeze dryer in a dark environment, carrying out vacuum freezing for 1.5h at the temperature of minus 20 ℃, carrying out sublimation drying for 2h at the temperature of 45 ℃, wherein the vacuum degree in the whole process is minus 0.01Mpa, and then crushing and sieving by a 35-mesh sieve to obtain green bonnie flower powder;
secondly, placing the scindapsus aureus pollen into a nonmetal closed container, and introducing negative hydrogen ion gas into the closed container, wherein the concentration of the hydrogen ion gas in the closed container is 6 multiplied by 10 5 Per cm 3 Rotating a nonmetal stirring rod in a sealed container under 0.1Mpa for 1 hr to obtain processed scindapsus aureus powder and 75% ethanol solution, and extracting with ethanol at normal temperature and pressure2 times, the material-liquid ratio of the first alcohol extraction is 1;
and step three, carrying out water extraction on the filter residue by using hot water at the temperature of 80 ℃, wherein the water extraction frequency is 2 times, and the mass ratio of the filter residue subjected to the first water extraction to the water is 1:5, the mass ratio of filter residue obtained by secondary water extraction to water is 1:10, the water extraction time is 1 hour each time; and mixing the two water extract filtrates to obtain a second filtrate, wherein the volume ratio of the second filtrate to 95% ethanol is 1:12, standing the mixed solution for 8 hours, filtering and collecting an alcohol precipitation solution, combining the alcohol precipitation solution and the first filtrate, and distilling to obtain the extract.
Example 4 animal experiments of the effect of the extract on lowering blood pressure were as follows:
1. specification and feeding requirements of laboratory rats
Healthy male SD rats with 24 animals, SPF level and weight (175 +/-30) g, all experimental animals are raised in a controllable environment at room temperature of 18-24 ℃ and humidity of 40-50%, the animals eat and drink water freely during the experiment, and the circadian rhythm is normal.
2. Experimental methods
(1) Establishing hypertension rat model
24 healthy male SD rats are fed adaptively for 1 week, the blood pressure of the tail artery of each rat is measured by an ALC-NIBP noninvasive blood pressure determination and analysis system before model building, the measurement is repeated for 3 times, and the average value is taken and recorded. A hypertension rat model is prepared by adopting a 'two kidneys and one clamp' method. After 4 weeks of molding, the blood pressure of 24 rats meets the Systolic Pressure (SP) which is more than 120mmHg, and the blood pressure after molding is more than 20mmHg higher than that before molding, namely the experimental hypertension rats.
(2) Experimental grouping and administration mode
And taking 24 hypertension rats successfully modeled, randomly dividing the rats into 4 groups, and each group comprises 6 rats, namely a model control group, an extract high-dose group, an extract medium-dose group and an extract low-dose group. After the model is successfully established for 4 weeks, and after the SP of the rat is stabilized, the ALC-NIBP noninvasive blood pressure determination and analysis system is used for determining the tail artery blood pressure of the rat in the waking state before administration. The extract content in the high, medium and low dose groups is respectively 5ml/kg, 10ml/kg and 15ml/kg, and the administration is performed by intragastric administration, wherein the rats in the model control group are administered with physiological saline with the same amount as that in intragastric administration every day, and the administration is performed 1 time every day starting at week 5 and continuously for 4 weeks. Wherein, the dosage of the same amount of normal saline is the same as that of the abdominal cavity.
3. Blood pressure measurement
Before modeling, at the 4 th week of modeling and at the 4 th week of administration, the caudal arterial Systolic Pressure (SP) of the rat in the awake state is measured by an ALC-NIBP noninvasive blood pressure measurement and analysis system respectively, the measurement is repeated for 3 times, the average value is taken, and the obtained data is detailed in Table 2.
Table 2: rat blood pressure changes (
mmHg)
Note: the self blood pressure before and after the administration is compared, △ P<0.05， △△ P<0.01; compared with the model control group, * P<0.05， ** P<0.01。
the data in Table 2 show that the extract is effective in lowering blood pressure in hypertensive rats. After the hypertension rats successfully modeled are administrated for 4 weeks, the high, medium and low dose groups of the extract have obvious blood pressure reducing effects, and compared with a model control group, the high, medium and low dose groups of the extract in the example 4 (5 ml/kg, 10ml/kg and 15 ml/kg) have obvious blood pressure reducing effects compared with the self blood pressure before and after the administration, wherein the blood pressure of the high, medium and low dose groups of the extract is obviously reduced. The scindapsus aureus flower powder is mixed with the negative hydrogen ion gas before alcohol extraction, and partially oxidized compounds such as coumarins, flavonoids and the like in the crushing process are reduced, so that the extraction effect and activity of the extract are improved, and the blood pressure reducing effect in animal experiments is obviously improved.
< example 5>
An extraction process of scindapsus aureus, which comprises the following steps:
step one, putting the cleaned green bonnie flower in a vacuum freeze dryer in a dark environment, carrying out vacuum freezing for 1.5h at the temperature of minus 20 ℃, carrying out sublimation drying for 2h at the temperature of 45 ℃, wherein the vacuum degree in the whole process is minus 0.01Mpa, and then crushing and sieving by a 35-mesh sieve to obtain green bonnie flower powder;
secondly, placing the scindapsus aureus pollen into a nonmetal closed container, and introducing negative hydrogen ion gas into the closed container, wherein the concentration of the hydrogen ion gas in the closed container is 6 multiplied by 10 5 Per cm 3 The pressure is 0.1Mpa, the nonmetal stirring rod in the closed container is rotated for 1 hour to obtain treated scindapsus aureus powder and 75% ethanol solution, the scindapsus aureus powder is subjected to ethanol extraction for 2 times under normal temperature and pressure, the material-liquid ratio of the first ethanol extraction is 1;
and step three, carrying out water extraction on the filter residue by using hot water at the temperature of 80 ℃, wherein the water extraction frequency is 2 times, and the mass ratio of the filter residue subjected to the first water extraction to the water is 1:5, the mass ratio of filter residue obtained by secondary water extraction to water is 1:10, each water extraction time is 1 hour; and mixing the two water extract filtrates to obtain a second filtrate, wherein the volume ratio of the second filtrate to 95% ethanol is 1:12 mixing, standing for 8 hr, filtering, collecting ethanol precipitation solution, mixing with the first filtrate, placing in a distillation flask, and introducing negative hydrogen ion gas with concentration of 6 × 10 5 Per cm 3 Negative hydrogen ion gas is introduced from the bottom of the distillation flask, a plurality of air outlets are uniformly distributed on an air pipe at the bottom of the distillation flask and used for allowing the negative hydrogen ion gas to flow into the solution from the air outlets, so that the negative hydrogen ion gas is mixed in the solution, and then the emitted gas is extracted from the top of the distillation flask to form circulating negative hydrogen ion gas which is distilled to obtain the extract.
Example 5 animal experiments of the effect of extracts on lowering blood pressure were as follows:
1. specification and feeding requirements of laboratory rats
The male SD rat is 24 male SD rats, the SPF level is SPF, the weight is 175 +/-30 g, all experimental animals are raised in a controllable environment, the room temperature is 18-24 ℃, the humidity is 40% -50%, the animals eat and drink water freely during the experiment, and the circadian rhythm is normal.
2. Experimental methods
(1) Establishing hypertension rat model
24 healthy male SD rats are fed adaptively for 1 week, the blood pressure of the tail artery of each rat is measured by an ALC-NIBP noninvasive blood pressure determination and analysis system before model building, the measurement is repeated for 3 times, and the average value is taken and recorded. A hypertension rat model is prepared by adopting a 'two kidneys and one clip' method. After 4 weeks of molding, the blood pressure of 24 rats meets the Systolic Pressure (SP) which is more than 120mmHg, and the blood pressure after molding is more than 20mmHg higher than that before molding, namely the experimental hypertension rats.
(2) Experimental grouping and administration mode
And (3) randomly dividing 24 hypertension rats successfully molded into 4 groups, wherein each group comprises 6 rats, namely a model control group, an extract high-dose group, an extract medium-dose group and an extract low-dose group. After the model is successfully established for 4 weeks, after the SP of the rat is stabilized, the blood pressure of the tail artery of the rat in the waking state is determined by an ALC-NIBP noninvasive blood pressure determination analysis system before administration. The extract content in the high, medium and low dose groups is respectively 5ml/kg, 10ml/kg and 15ml/kg, and the administration is performed by intragastric administration, wherein the rats in the model control group are administered with physiological saline with the same amount as that in intragastric administration every day, and the administration is performed 1 time every day starting at week 5 and continuously for 4 weeks. Wherein, the equivalent amount of normal saline is the same as the administration amount of abdominal cavity.
3. Blood pressure measurement
Before modeling, at the 4 th week of modeling and at the 4 th week of administration, the caudal arterial Systolic Pressure (SP) of the rat in the awake state is measured by an ALC-NIBP noninvasive blood pressure measurement and analysis system respectively, the measurement is repeated for 3 times, the average value is taken, and the obtained data is detailed in Table 3.
Table 3: rat blood pressure changes (
mmHg)
Note: the self blood pressure before and after the administration is compared, △ P<0.05， △△ P<0.01; compared with the model control group, * P<0.05， ** P<0.01。
the data in Table 3 show that the extract is effective in lowering blood pressure in hypertensive rats. After the hypertension rats successfully modeled are administrated for 4 weeks, the high, medium and low dose groups of the extract have obvious blood pressure reducing effects, and compared with a model control group, the high, medium and low dose groups of the extract in example 5 (5 ml/kg, 10ml/kg and 15 ml/kg) have obvious blood pressure reducing effects compared with the self blood pressure before and after the administration, wherein the blood pressure of the high, medium and low dose groups of the extract is obviously reduced. According to the invention, the green bonnie flower powder and the negative hydrogen ion gas are mixed before alcohol extraction, and the negative hydrogen ion gas is circularly introduced into the solution during distillation for mixing, so that the extraction effect and activity of the extract can be further improved, and the blood pressure reducing effect in animal experiments is further obviously improved.
The extraction rates of the edgeworthiness C and the edgeworthiness C in the green bonnie flowers extracted in examples 1, 4 and 5 were calculated by using separation means such as silica gel column chromatography, sephadex LH-20 and recrystallization, wherein the extraction rate = weight of the edgeworthiness C or the edgeworthiness C in the fixed amount of the extract/fixed amount of the extract × 100%, the fixed amount of the extract is taken as 100g, and the calculation results are shown in the following table 4.
Table 4:
| categories | Extraction ratio (%) of edgeworthia chrysantha C | Extraction ratio of knot essence (%) |
| Example 1 | 6.39 | 7.52 |
| Example 4 | 9.84 | 8.73 |
| Example 5 | 11.97 | 10.44 |
The data in table 4 clearly show that the extraction effect of the extract can be further improved by mixing the green bonnie flower powder with the negative hydrogen ion gas before the alcohol extraction and circulating and introducing the negative hydrogen ion gas into the solution during the distillation.
While embodiments of the invention have been disclosed above, it is not intended to be limited to the uses set forth in the specification and examples. It can be applied to all kinds of fields suitable for the present invention. Additional modifications will readily occur to those skilled in the art.
Claims (7)
1. The scindapsus aureus flower extraction process is characterized by comprising the following steps:
step one, preparing the scindapsus aureus into powder;
secondly, placing the scindapsus aureus pollen into a nonmetal closed container, and introducing negative hydrogen ion gas into the closed container, wherein the concentration of the hydrogen ion gas in the closed container is 6 multiplied by 10 5 Per cm 3 Rotating the non-metal stirring rod in the sealed container for 1-2 hr under 0.1 MPa; then extracting the treated green bonnie flower powder and an ethanol solution with the concentration of 70-85% for 1-2.5 hours according to the material-liquid ratio of 1 to 10-15g/ml, and filtering the ethanol extract to obtain a first filtrate and filter residues for later use;
and step three, extracting the filter residue with hot water, filtering the water extract to obtain a second filtrate, wherein the volume ratio of the second filtrate to 90-95% ethanol is 1:10-15, standing, filtering and collecting an alcohol precipitation solution,mixing the ethanol precipitation solution and the first filtrate, and distilling to obtain extract; wherein, when the alcohol precipitation solution and the first filtrate are combined and distilled, negative hydrogen ion gas is circularly introduced into the solution, and the concentration of the negative hydrogen ion gas is 6 multiplied by 10 5 Per cm 3 Negative hydrogen ion gas is introduced from the bottom of the distillation flask, a plurality of air outlets are uniformly distributed on an air pipe at the bottom of the distillation flask and used for the negative hydrogen ion gas to flow into solution from the air outlets, so that the negative hydrogen ion gas is mixed in the solution, and the emitted gas is pumped out from the top of the distillation flask to form circulation.
2. The scindapsus aureus extraction process according to claim 1, wherein the temperature of hot water is 70-100 ℃, the water extraction frequency of filter residue is 2 times, and the mass ratio of the filter residue obtained by the first water extraction to the water is 1:5, the mass ratio of filter residue obtained by secondary water extraction to water is 1:10, the water extraction time is 1-2 hours each time.
3. The scindapsus aureus flower extraction process of claim 2, wherein the resting time is 5-10 hours.
4. The scindapsus aureus extraction process of claim 3, wherein the first filtrate is extracted with alcohol for 2 times, the first alcohol extraction time is 1.5 hours, and the second alcohol extraction time is 1 hour.
5. The scindapsus aureus flower extraction process of any one of claims 1-4, wherein the scindapsus aureus flower powder is prepared by the following method: and (3) carrying out vacuum freeze drying on the cleaned scindapsus aureus in a dark environment, and crushing the scindapsus aureus in a vacuum environment after drying to obtain scindapsus aureus powder.
6. The scindapsus aureus flower extraction process of claim 5, wherein the vacuum freeze-drying is performed for 1.5 hours at-20 ℃, for 2 hours at 45 ℃ and under-0.01 MPa, and the scindapsus aureus flower is crushed and sieved with a 35-mesh sieve.
7. The application of the scindapsus aureus extract extracted by the process as claimed in claim 6, characterized in that the scindapsus aureus extract is used for preparing a hypotensive drug.
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