CN106071018A - A kind of pressed candy with antitumor action and preparation method and application - Google Patents
A kind of pressed candy with antitumor action and preparation method and application Download PDFInfo
- Publication number
- CN106071018A CN106071018A CN201610553073.2A CN201610553073A CN106071018A CN 106071018 A CN106071018 A CN 106071018A CN 201610553073 A CN201610553073 A CN 201610553073A CN 106071018 A CN106071018 A CN 106071018A
- Authority
- CN
- China
- Prior art keywords
- powder
- sulforaphen
- pressed candy
- semen raphani
- antitumor action
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- XFZJEEAOWLFHDH-NFJBMHMQSA-N procyanidin B2 Chemical compound C1([C@@H]2[C@H](O)[C@H](C3=C(O)C=C(O)C=C3O2)C=2C(O)=CC(O)=C3C[C@H]([C@H](OC3=2)C=2C=C(O)C(O)=CC=2)O)=CC=C(O)C(O)=C1 XFZJEEAOWLFHDH-NFJBMHMQSA-N 0.000 claims abstract description 58
- QKGJFQMGPDVOQE-UHFFFAOYSA-N Sulforaphen Natural products CS(=O)C=CCCN=C=S QKGJFQMGPDVOQE-UHFFFAOYSA-N 0.000 claims abstract description 50
- QKGJFQMGPDVOQE-HWKANZROSA-N raphanin Chemical compound CS(=O)\C=C\CCN=C=S QKGJFQMGPDVOQE-HWKANZROSA-N 0.000 claims abstract description 50
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims abstract description 34
- XFZJEEAOWLFHDH-UHFFFAOYSA-N (2R,2'R,3R,3'R,4R)-3,3',4',5,7-Pentahydroxyflavan(48)-3,3',4',5,7-pentahydroxyflavan Natural products C=12OC(C=3C=C(O)C(O)=CC=3)C(O)CC2=C(O)C=C(O)C=1C(C1=C(O)C=C(O)C=C1O1)C(O)C1C1=CC=C(O)C(O)=C1 XFZJEEAOWLFHDH-UHFFFAOYSA-N 0.000 claims abstract description 30
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- MOJZMWJRUKIQGL-FWCKPOPSSA-N Procyanidin C2 Natural products O[C@@H]1[C@@H](c2cc(O)c(O)cc2)Oc2c([C@H]3[C@H](O)[C@@H](c4cc(O)c(O)cc4)Oc4c3c(O)cc(O)c4)c(O)cc(O)c2[C@@H]1c1c(O)cc(O)c2c1O[C@@H]([C@H](O)C2)c1cc(O)c(O)cc1 MOJZMWJRUKIQGL-FWCKPOPSSA-N 0.000 description 1
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Classifications
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- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/48—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing plants or parts thereof, e.g. fruits, seeds, extracts
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- A—HUMAN NECESSITIES
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- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
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- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/42—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds characterised by the carbohydrates used, e.g. polysaccharides
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- A23G2200/06—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF containing organic compounds, e.g. synthetic flavouring agents containing beet sugar or cane sugar if specifically mentioned or containing other carbohydrates, e.g. starches, gums, alcohol sugar, polysaccharides, dextrin or containing high or low amount of carbohydrate
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- A—HUMAN NECESSITIES
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- A23G2200/00—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF containing organic compounds, e.g. synthetic flavouring agents
- A23G2200/14—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF containing organic compounds, e.g. synthetic flavouring agents containing fruits, nuts, e.g. almonds, seeds, plants, plant extracts or essential oils
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2022—Organic macromolecular compounds
- A61K9/205—Polysaccharides, e.g. alginate, gums; Cyclodextrin
- A61K9/2059—Starch, including chemically or physically modified derivatives; Amylose; Amylopectin; Dextrin
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Inorganic Chemistry (AREA)
- Veterinary Medicine (AREA)
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Abstract
The present invention relates to food technology field, specifically disclose a kind of pressed candy containing sulforaphen and procyanidin B 2 with antitumor action and preparation method thereof.Pressed candy includes following component: sulforaphen powder, procyanidin B 2, maltodextrin, microcrystalline Cellulose, magnesium stearate and sweeting agent, each component is prepared by tabletting after mixing.The present invention passes through procyanidin B 2 and the synergism of sulforaphen, improve both stability, they are respectively by the way of directly or indirectly, continue to play antioxidation, the effect of removing interior free yl efficiently, and demonstrate inhibition significant to tumor.
Description
Technical field
The present invention relates to food technology field, particularly relate to a kind of have antitumor action containing sulforaphen and former flower
Pressed candy of blue or green element B2 and preparation method and application.
Background technology
The at present aggravation of air pollution, the bad life habits etc. such as day by day serious and smoking of food-safety problem cause
Human body produces substantial amounts of free radical.Free radical can make lipid aoxidize, and damages cell membrane；With protein molecular effect, destroy
The structure of the normal the most various enzyme of albumen, causes it can not normally play physiological function；With DNA molecular effect, lesioned gene,
Cause cytometaplasia.These ill effects of free radical can cause aging, inflammation, diabetes, cardiovascular disease and cancer
Etc. various diseases, therefore remove interior free yl and can effectively protect health.
Sulforaphen is a kind of isosulfocyanate compound of isolated from natural product, and it can pass through
The endonuclear Antioxidant responsive element of Keap1/Nrf2 pathway activation, induces multiple antioxidation albumen and the table of II phase detoxication enzyme
Reach.Antioxidation albumen can free radical in scavenger cell, maintain the oxidation level of cell, prevent it by the damage of active oxygen etc.
Evil.II phase enzyme has Detoxication, it is possible to effectively protection body is from carcinogenic injury, thus plays the work of cancer-resisting
With.
Procyanidin B 2 is by a kind of polyphenolic substance of two molecule catechin condensations, has the strongest antioxidation and lives
Property and free radical scavenging function.Discharge H+ after it is the most oxidized, can be combined with free radical and oxide competitively, from
And protect lipid not oxidized, block free chain reaction.
Although sulforaphen and procyanidin B 2 have effect as above, but both stability is the best, easily divides
Solving, therefore, for sulforaphen and two kinds of materials of procyanidin B 2, the present invention proposes a kind of pressed candy with antitumor action
Really.
Summary of the invention
In view of this, it is an object of the invention to overcome the deficiencies in the prior art, it is provided that a kind of stability is good, when storing
Between the long pressed candy with antitumor action and preparation method and application.
In order to solve above-mentioned technical problem, the present invention uses following scheme to realize:
A kind of pressed candy with antitumor action, including following component: sulforaphen powder, procyanidin B 2, maltodextrin,
Microcrystalline Cellulose, magnesium stearate and sweeting agent.
Procyanidin B 2 has the strongest antioxidant activity and a free radical scavenging function, but due to procyanidin B 2 point
Minor structure reason, its stability is not enough, is easily subject to external influence and degrades, and this is also that it is subject to apply the weight limited
Want reason.Applicant carried out patent application previously for sulforaphen, and such as application number 201510728763.2, this patent is passed through
The method that sulforaphen crude extract is prepared as pressed candy, the problem solving sulforaphen stability, therefore inventor for
Patent before this replaces with procyanidin B 2 to sulforaphen crude extract, it is desirable to realize improving procyanidin B 2 stability by this
Purpose, to realize more permanent holding time, but result shows, after replacing with procyanidin B 2, does not solve former flower
The problem of blue or green element B2 stability.Inventor is by discovery, trailing plants after being mixed with procyanidin B 2 by a certain amount of sulforaphen powder
Foretelling and do not suppress between thionin powder and procyanidin B 2, both stability is greatly improved, and not only extends storage
The time deposited, and both match respectively by the way of indirectly and directly, continue to play efficiently in antioxidation, purged body
The effect of free radical, further promotes the antitumor action of sulforaphen powder.
Described sulforaphen powder, procyanidin B 2, maltodextrin, microcrystalline Cellulose, magnesium stearate and the weight ratio of sweeting agent
It is 2.0 ~ 2.5:0.2:1.8 ~ 2.3:0.5:0.03:0.005.
Research finds, sulforaphen powder and procyanidin cooperate and can improve both stability within the specific limits, but
When the amount of sulforaphen powder be the amount of procyanidin B 2 more than 15 times, its stability is but greatly reduced.Therefore, the present invention exists
The ratio range of optimum has been drawn on the basis of great many of experiments.
Further, described sulforaphen powder, procyanidin B 2, maltodextrin, microcrystalline Cellulose, magnesium stearate and sweet taste
The weight ratio of agent is 2.3:0.2:2.0:0.5:0.03:0.005.
The preparation method of a kind of pressed candy with antitumor action, comprises the steps:
S10: by proportioning mixing sulforaphen powder, procyanidin B 2, maltodextrin, microcrystalline Cellulose, magnesium stearate and sweeting agent；
S11: each component of mixing in step S10 is carried out tabletting and prepares pressed candy.The process conditions of tableting processes are according to existing
Technology is had to regulate.
Described sulforaphen powder is prepared by the following method:
S20: Radix Raphani concentrates powder, broccoli concentrated powder and defat Semen Raphani concentrate powder and mix mutually and to obtain vegetable and concentrate powder；
S21: the vegetable in step S20 is concentrated after powder extracts and concentrates, it is thus achieved that thioglycoside extractum；
S22: the thioglycoside extractum obtained in step S21 is carried out enzymolysis, obtains sulforaphen extracting solution after filtration；
S23: the sulforaphen extracting solution obtaining step S22 pulverizes to obtain sulforaphen powder after carrying out lyophilization.
Radix Raphani concentrate powder be prepared via a method which: by big Radix Dauci Sativae remove the peel after dewatered drying to constant weight, after pulverized also
Sieve to obtain Radix Raphani concentration powder；
Broccoli concentrated powder is prepared via a method which: by Caulis et Folium Brassicae capitatae spray dehydration post-drying to constant weight, after pulverized and mistake
Sieve to obtain broccoli concentrated powder；
Defat Semen Raphani concentrates powder and is prepared via a method which: Semen Raphani powder of pulverizing after Semen Raphani natural air drying and sieve to obtain,
Use normal hexane that Semen Raphani powder carries out ungrease treatment and obtain Semen Raphani granulated slag, then Semen Raphani granulated slag crushed after being dried is obtained defat
Semen Raphani concentrates powder.
Described ungrease treatment includes normal hexane defat at least twice: be placed in infuser by Semen Raphani powder and pump into just own
Alkane carries out defat for the first time, releases leachate afterwards；Again pump into normal hexane and carry out second time defat, then pass to compressed air straight
Semen Raphani granulated slag is no longer flowed out to obtain to leachate.
Further, in step S21, in step S21, described extraction includes alcohol steep at least twice: concentrated by vegetable
Powder is scattered in 95% ethanol and carries out extracting for the first time, carries out sucking filtration after having extracted for the first time；Filtering residue after extraction for the first time
In again add 95% ethanol carry out second time extract, carry out sucking filtration the most again；Vacuum is carried out after the filtrate of twice extraction being merged
Concentrate and obtain thioglycoside extractum.
Further, in step S22, described enzyme solution is: defat Semen Raphani concentration powder is joined pH is 2.0 ~ 3.5
Citrate buffer solution in stir, then mix with thioglycoside extractum and carry out enzymolysis.
The present invention, by carrying out enzymolysis again after first condensate precursor thioglycoside, improves the enzymolysis efficiency of sulforaphen
And yield.
Compared with prior art, there is advantages that the present invention passes through procyanidin B 2 and sulforaphen
Synergism, improves both stability, and they continue to play efficiently antioxygen respectively by the way of directly or indirectly
Change, remove the effect of interior free yl, and demonstrate inhibition significant to tumor.
Detailed description of the invention
In order to allow those skilled in the art be more fully understood that technical scheme, below the present invention is made further
Illustrate.
Embodiment 1
One, the preparation of pressed candy
A kind of pressed candy with antitumor action, including following each component: sulforaphen powder, procyanidin B 2, Fructus Hordei Germinatus are stuck with paste
Essence, microcrystalline Cellulose, magnesium stearate and xylitol, the mass ratio between each component is 2.0:0.2:1.8:0.5:0.03:
0.005。
Described pressed candy is made by the steps:
S10: by proportioning mixing sulforaphen powder, procyanidin B 2, maltodextrin, microcrystalline Cellulose, magnesium stearate and xylitol；
S11: each component of mixing in step S10 is carried out tabletting and prepares pressed candy.
Wherein, in step S10, described sulforaphen powder is prepared via a method which:
S20: Radix Raphani is concentrated powder, broccoli concentrated powder and the defat Semen Raphani concentration powder ratio with mass ratio as 1:1:4 and mixes mutually
Obtain vegetable and concentrate powder；
It is the peeling of fresh big Radix Dauci Sativae, stripping and slicing that described Radix Raphani concentrates powder, is dehydrated 1h, then dries at 50 DEG C to permanent at 70 DEG C
Weight, obtain dry radish block, dry radish block pulverized, then with the stainless steel mesh of 40 mesh carry out screening obtain Radix Raphani concentration powder；
Described broccoli concentrated powder is that the spray of fresh broccoli is dehydrated 1h at 70 DEG C, then dries to constant weight at 50 DEG C,
Obtain the dry block of Caulis et Folium Brassicae capitatae, dry for Caulis et Folium Brassicae capitatae block is pulverized, then carries out screening with the stainless steel mesh of 40 mesh and obtain broccoli concentrated powder；
It is by fresh Semen Raphani natural air drying that described defat Semen Raphani concentrates powder, then pulverizes, with the stainless steel mesh of 40 mesh
Sieving, collect the Semen Raphani powder after sieving, be placed in pot type infuser, pump into normal hexane, controlling temperature is 20 DEG C,
Stirring defat 30min, releases leachate, again pumps into normal hexane, and controlling temperature is 20 DEG C, stirs defat 30min, then leads to
Enter gauge pressure be 0.1MPa, flow velocity be the compressed air of 5m/s, till leachate no longer flows out, obtain Semen Raphani granulated slag, by Lay
Fu granulated slag vacuum constant temperature at 30 DEG C is dried 1h, again pulverizes, and obtains defat Semen Raphani and concentrates powder.
S21: the vegetable in step S20 is concentrated after powder extracts and concentrates, it is thus achieved that thioglycoside extractum；
Described extraction method for concentration is as follows: vegetable concentrates powder and is scattered in 95% ethanol, carries out for the first time at a temperature of 65 DEG C
30min is extracted in stirring, and wherein vegetable concentrates the volume ratio of opaque amount and 95% ethanol is 1.0g:10ml；After having extracted for the first time
Carry out sucking filtration, filtering residue adds 95% ethanol and again extracts, then carry out sucking filtration；The filtrate of twice extraction is merged
It is concentrated in vacuo at 45 DEG C, obtains thioglycoside extractum.
S22: the thioglycoside extractum obtained in step S21 is carried out enzymolysis, obtains sulforaphen and extracts after filtration
Liquid；
Described enzyme solution is as follows: Semen Raphani above-mentioned steps prepared concentrates powder and is scattered in the citrate buffer solution of pH2.0,
Stir, be then added to thioglycoside extractum, then at 20 DEG C, enzymolysis 6h, enzymolysis under the mixing speed of 100r/min
Sulforaphen extracting solution is filtered to obtain after completing；Thioglycoside extractum, defat Semen Raphani concentrate powder, the use of citrate buffer solution
Amount ratio is 1ml:3g:10ml.
S23: step S22 is obtained sulforaphen extracting solution 25Pa vacuum, at-50 DEG C lyophilization to constant weight, pulverize
Sulforaphen powder is obtained to 60 mesh.
Two, zoopery
1, experiment material
C57BL/6J mice, female, SPF level, body weight 18 ± 1g, Beijing dimension tonneau China Experimental Animal Center；
Cyclophosphamide, Shanghai Jing Chun biochemical technology limited company；
The present embodiment pressed candy (has stored 24 months), 500mg/ sheet.
2, experimental technique
Collect Lewis lung cancer cell, make single cell suspension, take 0.2mL(containing oncocyte 3 × 107) it is inoculated in the right axillary fossa of mice
Subcutaneous.Inoculate next day, mice is randomly divided into negative control group, experimental group and positive controls, often group 10.Pressure slab sugar
Really, grind to form fine powder, make suspension by every 1mL distilled water.Experimental group every Mus gavage 0.5mL every day suspension, negative right
According to organizing every Mus gavage 0.5mL every day distilled water, positive controls gives cyclophosphamide, continuously by every Mus 10mg/kg every day dosage
10 days.Period routine is raised, and drinking-water, food do not limit.Drug withdrawal next day, mice all takes off cervical vertebra and puts to death, peels off subcutaneous solid tumors tumor
Block, weighs, by following equation calculating tumor control rate (%):
Experimental group tumor control rate/%=[(negative control group average tumor weight-experimental group average tumor weight)/negative control group is average
Tumor weight] × 100%；
Positive controls tumor control rate/%=[(negative control group average tumor weight-positive controls average tumor weight)/negative right
According to organizing average tumor weight] × 100%.
3, experimental result
Experimental result for the first time
| Group | Tumor control rate (%) |
| Experimental group | 65.5 |
| Positive controls | 30.3 |
Second time repeats experimental result
| Group | Tumor control rate (%) |
| Experimental group | 67.2 |
| Positive controls | 33.6 |
Embodiment 2
One, the preparation of pressed candy
A kind of pressed candy with antitumor action, including following each component: sulforaphen powder, procyanidin B 2, Fructus Hordei Germinatus are stuck with paste
Essence, microcrystalline Cellulose, magnesium stearate and xylitol, the mass ratio between each component is 2.5:0.2:2.3:0.5:0.03:
0.005。
Described pressed candy is made by the steps:
S10: by proportioning mixing sulforaphen powder, procyanidin B 2, maltodextrin, microcrystalline Cellulose, magnesium stearate and xylitol；
S11: each component of mixing in step S10 is carried out tabletting and prepares pressed candy.
Wherein, in step S10, described sulforaphen powder is prepared via a method which:
S20: Radix Raphani is concentrated powder, broccoli concentrated powder and the defat Semen Raphani concentration powder ratio with mass ratio as 1:1:6 and mixes mutually
Obtain vegetable and concentrate powder；
It is the peeling of fresh big Radix Dauci Sativae, stripping and slicing that described Radix Raphani concentrates powder, is dehydrated 2h, then dries at 60 DEG C to permanent at 80 DEG C
Weight, obtain dry radish block, dry radish block pulverized, then with the stainless steel mesh of 60 mesh carry out screening obtain Radix Raphani concentration powder；
Described broccoli concentrated powder is that the spray of fresh broccoli is dehydrated 2h at 80 DEG C, then dries to constant weight at 60 DEG C,
Obtain the dry block of Caulis et Folium Brassicae capitatae, dry for Caulis et Folium Brassicae capitatae block is pulverized, then carries out screening with the stainless steel mesh of 60 mesh and obtain broccoli concentrated powder；
It is by fresh Semen Raphani natural air drying that described defat Semen Raphani concentrates powder, then pulverizes, with the stainless steel mesh of 60 mesh
Sieving, collect the Semen Raphani powder after sieving, be placed in pot type infuser, pump into normal hexane, controlling temperature is 40 DEG C,
Stirring defat 60min, releases leachate, again pumps into normal hexane, and controlling temperature is 40 DEG C, stirs defat 60min, then leads to
Enter gauge pressure be 0.4MPa, flow velocity be the compressed air of 15m/s, till leachate no longer flows out, obtain Semen Raphani granulated slag, by Lay
Fu granulated slag vacuum constant temperature at 40 DEG C is dried 2h, again pulverizes, and obtains defat Semen Raphani and concentrates powder.
S21: the vegetable in step S20 is concentrated after powder extracts and concentrates, it is thus achieved that thioglycoside extractum；
Described extraction method for concentration is as follows: vegetable concentrates powder and is scattered in 95% ethanol, carries out for the first time at a temperature of 75 DEG C
60min is extracted in stirring, and wherein vegetable concentrates the volume ratio of opaque amount and 95% ethanol is 1.0g:15ml；After having extracted for the first time
Carry out sucking filtration, filtering residue adds 95% ethanol and again extracts, then carry out sucking filtration；The filtrate of twice extraction is merged
It is concentrated in vacuo at 55 DEG C, obtains thioglycoside extractum.
S22: the thioglycoside extractum obtained in step S21 is carried out enzymolysis, obtains sulforaphen and extracts after filtration
Liquid；
Described enzyme solution is as follows: Semen Raphani above-mentioned steps prepared concentrates powder and is scattered in the citrate buffer solution of pH3.5,
Stir, be then added to thioglycoside extractum, then at 40 DEG C, enzymolysis 9h, enzymolysis under the mixing speed of 200r/min
Sulforaphen extracting solution is filtered to obtain after completing；Thioglycoside extractum, defat Semen Raphani concentrate powder, the use of citrate buffer solution
Amount ratio is 1ml:5g:20ml.
S23: step S22 is obtained sulforaphen extracting solution 45Pa vacuum, at-60 DEG C lyophilization to constant weight, pulverize
Sulforaphen powder is obtained to 60 mesh.
Two, zoopery
1, experiment material
BALB/c nude mice, male, SPF level, body weight 18-20g, Guangdong Medical Lab Animal Center；
BxPC-3 human pancreatic cancer cell, Shanghai Chinese Academy of Sciences cell bank；
Hydrochloride for injection gemcitabine (gemzar), ELI LILLY AND COMPANY
The present embodiment pressed candy (has stored 24 months), 500mg/ sheet.
2, experimental technique
BxPC-3 human pancreatic cancer cell after Secondary Culture, the cell of trophophase of taking the logarithm, be prepared as 5 × 10 with PBS7Cell/
The cell suspension of ml concentration, be inoculated in nude mice armpit subcutaneous (every mice 100 μ l, altogether 5 × 106Cell).
By Mouse feeder about 10 days, screening gross tumor volume reached 100-200mm3Tumor bearing nude mice 24, random district group
Method is divided into negative control group, positive controls and experimental group, often group 8.Ensure that between each group, gross tumor volume and Mouse Weight are homogeneous.
The average of each group gross tumor volume and the average difference of all experimental animal tumor volumes are less than ± 10%.
Take pressed candy, grind to form fine powder, make suspension by every 1mL distilled water.After being grouped, the every Mus of experimental group
Every day gavage 0.5mL suspension, negative control group every Mus gavage 0.5mL every day distilled water, positive controls is pressed by every Mus every time
50mg/kg dosage lumbar injection gemcitabine, biweekly.It is administered and altogether continues 3 weeks.Period routine is raised, and drinking-water, food are not
Limit.Drug withdrawal next day, mice all takes off cervical vertebra and puts to death, peel off subcutaneous solid tumors tumor mass, weigh, calculates tumor suppression by following equation
Rate (%):
Experimental group tumor control rate/%=[(negative control group average tumor weight-experimental group average tumor weight)/negative control group is average
Tumor weight] × 100%；
Positive controls tumor control rate/%=[(negative control group average tumor weight-positive controls average tumor weight)/negative right
According to organizing average tumor weight] × 100%.
3, experimental result
Experimental result for the first time
| Group | Tumor control rate (%) |
| Experimental group | 60.6 |
| Positive controls | 50.5 |
Second time repeats experimental result
| Group | Tumor control rate (%) |
| Experimental group | 64.3 |
| Positive controls | 53.2 |
Embodiment 3
One, the preparation of pressed candy
The present embodiment is similar to Example 1, and difference is, sulforaphen powder, procyanidin B 2, maltodextrin, microcrystalline Cellulose,
Magnesium stearate and xylitol, the mass ratio between each component is 2.3:0.2:2.0:0.5:0.03:0.005.
Two, zoopery
1, experiment material
BALB/c nude mice, female, SPF level, body weight 16-18g, Hunan Si Laike Jing Da laboratory animal company limited；
HepG2 human liver cancer cell, Shanghai Chinese Academy of Sciences cell bank；
Hydrochloride for injection doxorubicin, Haizheng Medicine Stock Co., Ltd., Zhejiang Prov；
The present embodiment pressed candy (has stored 24 months), 500mg/ sheet.
2, experimental technique
HepG2 human liver cancer cell after Secondary Culture, the cell of trophophase of taking the logarithm, be prepared as 1 × 10 with PBS7Cell/ml is dense
The cell suspension of degree, be inoculated in nude mice armpit subcutaneous (every mice 100 μ l, altogether 1 × 106Cell).
By Mouse feeder about 7 days, screening gross tumor volume reached 100-150mm3Tumor bearing nude mice 24, randomized blocks
It is divided into negative control group, positive controls and experimental group, often group 8.Ensure that between each group, gross tumor volume and Mouse Weight are homogeneous.Respectively
The average of group gross tumor volume and the average difference of all experimental animal tumor volumes are less than ± 10%.
Take pressed candy, grind to form fine powder, make suspension by every 1mL distilled water.After being grouped, the every Mus of experimental group
Every day gavage 0.5mL suspension, negative control group every Mus gavage 0.5mL every day distilled water, positive controls is pressed by every Mus every time
10mg/kg dosage lumbar injection doxorubicin, a Wednesday time.It is administered and altogether continues 3 weeks.Period routine is raised, and drinking-water, food are not
Limit.Drug withdrawal next day, mice all takes off cervical vertebra and puts to death, peel off subcutaneous solid tumors tumor mass, weigh, calculates tumor suppression by following equation
Rate (%):
Experimental group tumor control rate/%=[(negative control group average tumor weight-experimental group average tumor weight)/negative control group is average
Tumor weight] × 100%；
Positive controls tumor control rate/%=[(negative control group average tumor weight-positive controls average tumor weight)/negative right
According to organizing average tumor weight] × 100%.
3, experimental result
Experimental result for the first time
| Group | Tumor control rate (%) |
| Experimental group | 55.4 |
| Positive controls | 37.3 |
Second time repeats experimental result
| Group | Tumor control rate (%) |
| Experimental group | 56.2 |
| Positive controls | 32.5 |
Comparative example 1
This comparative example is similar to Example 1, and difference is, pressed candy includes following each component: sulforaphen powder, maltodextrin,
Microcrystalline Cellulose, magnesium stearate and xylitol, the mass ratio between each component is 2.0:1.8:0.5:0.03:0.005.
Experimental result is as follows:
Experimental result for the first time
| Group | Tumor control rate (%) |
| Experimental group | 58.5 |
| Positive controls | 32.5 |
Second time repeats experimental result
| Group | Tumor control rate (%) |
| Experimental group | 57.3 |
| Positive controls | 34.3 |
Comparative example 2
This comparative example is similar to Example 1, and difference is, pressed candy includes following each component: procyanidin B 2, Fructus Hordei Germinatus are stuck with paste
Essence, microcrystalline Cellulose, magnesium stearate and xylitol, the mass ratio between each component is 0.2:1.8:0.5:0.03:0.005.
Experimental result is as follows:
Experimental result for the first time
| Group | Tumor control rate (%) |
| Experimental group | 20.1 |
| Positive controls | 30.6 |
Second time repeats experimental result
| Group | Tumor control rate (%) |
| Experimental group | 18.2 |
| Positive controls | 31.7 |
Comparative example 3
This comparative example is similar to Example 1, and difference is, pressed candy includes following each component: sulforaphen powder, procyanidin
B2, maltodextrin, microcrystalline Cellulose, magnesium stearate and xylitol, the mass ratio between each component is 1.5:0.2:1.8:0.5:
0.03:0.005。
Experimental result is as follows:
Experimental result for the first time
| Group | Tumor control rate (%) |
| Experimental group | 40.6 |
| Positive controls | 34.5 |
Second time repeats experimental result
| Group | Tumor control rate (%) |
| Experimental group | 41.4 |
| Positive controls | 30.2 |
Comparative example 4
This comparative example is similar to Example 2, and difference is, pressed candy includes following each component: sulforaphen powder, procyanidin
B2, maltodextrin, microcrystalline Cellulose, magnesium stearate and xylitol, the mass ratio between each component is 3.0:0.2:2.3:0.5:
0.03:0.005。
Experimental result is as follows:
Experimental result for the first time
| Group | Tumor control rate (%) |
| Experimental group | 43.2 |
| Positive controls | 51.4 |
Second time repeats experimental result
| Group | Tumor control rate (%) |
| Experimental group | 42.8 |
| Positive controls | 52.3 |
Comparative example 5
This comparative example is similar with comparative example 4, and difference is, this comparative example pressed candy (has stored 6 months), 500mg/ sheet.
Experimental result is as follows:
Experimental result for the first time
| Group | Tumor control rate (%) |
| Experimental group | 54.7 |
| Positive controls | 52.6 |
Second time repeats experimental result
| Group | Tumor control rate (%) |
| Experimental group | 52.8 |
| Positive controls | 50.7 |
Above-described embodiment is only the wherein specific implementation of the present invention, therefore it describes more concrete and detailed, but can not be
And it is interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that, for the person of ordinary skill of the art, not
On the premise of departing from present inventive concept, it is also possible to making some deformation and improvement, these obvious alternative forms belong to
Protection scope of the present invention.
Claims (10)
1. a pressed candy with antitumor action, it is characterised in that include following component: sulforaphen powder, procyanidin
B2, maltodextrin, microcrystalline Cellulose, magnesium stearate and sweeting agent.
The pressed candy with antitumor action the most according to claim 1, it is characterised in that described sulforaphen powder, former
The weight ratio of anthocyanidin B2, maltodextrin, microcrystalline Cellulose, magnesium stearate and sweeting agent is 2.0 ~ 2.5:0.2:1.8 ~ 2.3:
0.5:0.03:0.005。
The pressed candy with antitumor action the most according to claim 2, it is characterised in that described sulforaphen powder, former
The weight ratio of anthocyanidin B2, maltodextrin, microcrystalline Cellulose, magnesium stearate and sweeting agent is 2.3:0.2:2.0:0.5:0.03:
0.005。
4. the preparation method of a pressed candy with antitumor action, it is characterised in that comprise the steps:
S10: mix sulforaphen powder, procyanidin B 2, maltodextrin, microcrystalline Cellulose, tristearin by the proportioning of Claims 2 or 3
Acid magnesium and sweeting agent；
S11: each component of mixing in step S10 is carried out tabletting and prepares pressed candy.
The preparation method of the pressed candy with antitumor action the most according to claim 4, it is characterised in that described trailing plants
Foretell thionin powder to prepare by the following method:
S20: Radix Raphani concentrates powder, broccoli concentrated powder and defat Semen Raphani concentrate powder and mix mutually and to obtain vegetable and concentrate powder；
S21: the vegetable in step S20 is concentrated after powder extracts and concentrates, it is thus achieved that thioglycoside extractum；
S22: the thioglycoside extractum obtained in step S21 is carried out enzymolysis, obtains sulforaphen extracting solution after filtration；
S23: the sulforaphen extracting solution obtaining step S22 pulverizes to obtain sulforaphen powder after carrying out lyophilization.
The preparation method of the pressed candy with antitumor action the most according to claim 5, it is characterised in that Radix Raphani is dense
Contracting powder is prepared via a method which: by big Radix Dauci Sativae remove the peel after dewatered drying to constant weight, after is pulverized and sieve to obtain Radix Raphani concentration
Powder；
Broccoli concentrated powder is prepared via a method which: by Caulis et Folium Brassicae capitatae spray dehydration post-drying to constant weight, after pulverized and mistake
Sieve to obtain broccoli concentrated powder；
Defat Semen Raphani concentrates powder and is prepared via a method which: Semen Raphani powder of pulverizing after Semen Raphani natural air drying and sieve to obtain,
Use normal hexane that Semen Raphani powder carries out ungrease treatment and obtain Semen Raphani granulated slag, then Semen Raphani granulated slag crushed after being dried is obtained defat
Semen Raphani concentrates powder.
The preparation method of the pressed candy with antitumor action the most according to claim 6, it is characterised in that described de-
Fat processes the normal hexane defat included at least twice: Semen Raphani powder is placed in infuser and pumps into normal hexane and carries out the most de-
Fat, releases leachate afterwards；Again pump into normal hexane and carry out second time defat, then pass to compressed air until leachate no longer flows
Go out to obtain Semen Raphani granulated slag.
The preparation method of the pressed candy with antitumor action the most according to claim 7, it is characterised in that step
In S21, described extraction includes alcohol steep at least twice: vegetable concentrates powder and is scattered in 95% ethanol and carries out extracting for the first time,
Sucking filtration is carried out after having extracted for the first time；Filtering residue after extraction for the first time adds 95% ethanol again carry out extracting for the second time,
Carry out sucking filtration the most again；Carry out after the filtrate of twice extraction being merged acquisition thioglycoside extractum is concentrated in vacuo.
The preparation method with the pressed candy seeing function of tumor the most according to claim 8, it is characterised in that step
In S22, described enzyme solution is: is joined in the citrate buffer solution that pH is 2.0 ~ 3.5 by defat Semen Raphani concentration powder and stirs
Uniformly, then with thioglycoside extractum mix and carry out enzymolysis.
10. the pressed candy described in any one of claims 1 to 3 is in the application of anti-tumor aspect.
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