CN109758492A - A kind of chrysanthemum total flavone and its preparation method and application - Google Patents
A kind of chrysanthemum total flavone and its preparation method and application Download PDFInfo
- Publication number
- CN109758492A CN109758492A CN201811494945.8A CN201811494945A CN109758492A CN 109758492 A CN109758492 A CN 109758492A CN 201811494945 A CN201811494945 A CN 201811494945A CN 109758492 A CN109758492 A CN 109758492A
- Authority
- CN
- China
- Prior art keywords
- chrysanthemum
- total flavone
- preparation
- fat
- chrysanthemum total
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The present invention provides a kind of chrysanthemum total flavone and its preparation method and application, is related to natural drug extractive technique field, the preparation method mixes after first crushing chrysanthemum with ethanol water, and water-bath obtains extraction mixture；To being separated by solid-liquid separation after extraction mixture ultrasound, chrysanthemum leaching liquor is obtained；Chrysanthemum leaching liquor is isolated and purified with macroporous absorbent resin, collects eluent, it is dry, obtain chrysanthemum total flavone.The present invention improves the recovery rate of effective component using ultrasonic extraction auxiliary, and the chrysanthemum total flavone purity reaches 84.5%, and chrysanthemum total flavone recovery rate reaches 5~7%.The embodiment of the present invention shows that the chrysanthemum total flavone of preparation can reduce rat blood serum TG, TC, LDL-C, NEFA level of induction high in fat, and elevating HDL-C is horizontal, reduces blood lipid and weight, can mitigate caused hepatic disease high in fat, reduce the accumulation of lipid in liver；With apparent weight-reducing and effect for reducing fat, can be applied in preparation weight-reducing or fat-reducing medicament.
Description
Technical field
The present invention relates to natural drug extractive technique field more particularly to a kind of chrysanthemum total flavone and preparation method thereof and answer
With.
Background technique
As obesity rates sharply increase, obesity has become the nutrition problem that a whole society pays close attention to jointly.It is fat and high
Rouge diet is related, this is because the imbalance between Energy intaking and consumption, caused by causing the accumulation of body fat, and with
Diabetes, hypertension and hyperlipidemia are closely related.Hyperlipemia be due to fat metabolism or operating it is abnormal keep blood plasma a kind of or
A variety of lipids are higher than normally, and lipid, which is insoluble or poorly soluble in water, to be existed in conjunction with protein with lipoprotein form, therefore, high in fat
Mass formed by blood stasis is often hyperlipoprotememia, shows as hypercholesterolemia, three vinegar mass formed by blood stasis of high glycerine or both and has concurrently.Hyperlipidemia can be brought
Many harm, this is induced Diabetic, atherosclerosis, coronary heart disease, the basic inducement of cholelithiasis and other diseases.
Most of diet products are containing cathartic ingredient currently on the market, and human body dehydration can lose weight certainly, but
It can make body electrolyte disturbance, lead to even more serious disease, furthermore some diet products are that people is allowed to generate satiety, to reduce
Food-intake is lost weight.But it does not eat or eats less and can cause malnutrition for a long time, to cause new disease.The treatment master of hyperlipidemia
Will be using chemicals such as Statins, fibrates, but these drug side-effects are larger.Therefore from natural plants chemicals ingredient
Seek to have become one of hot spot studied at present with the substance for adjusting and reducing fertilizer, effect for reducing fat.
Chrysanthemum belongs to the perennial herbaceous plant of dual-purpose of drug and food, is one of the medicinal herbs most in use of China's Traditional Chinese Medicine.FLOS CHRYSANTHEMI ALBA from Haizhou of China,
Also known as camomile, feverfew, tea chrysanthemum, medicine chrysanthemum not only have very high medical value, but also have effects that promote longevity.
Bunongshu carries: " camomile is sweet-warm, long term usage are most beneficial.", Yuan Dynasty's " a supplement to augmented materia medica " says: " tonifying yin of chrysanthemum energy ", Li Shizhen (1518-1593 A.D.)
Compendium of Material Medica road: sharp five arteries and veins of chrysanthemum energy adjust four limbs, control intermittent headache and vulcanize.The main component of chrysanthemum have volatile oil, gland he cry of certain animals, choline,
Stachydrine, chrysanthemum popularize flavone compound.Modern pharmacology research show chrysanthemum have it is anti-oxidant, remove free radical, it is anti-inflammatory,
It is antitumor, adjust blood pressure and blood lipoid the effects of.Flavone compound is considered as main active constituent in chrysanthemum, anti-with wide spectrum
Inflammation, scavenging activated oxygen, increases the multiple biological activities such as coronary blood flow, anti-oxidant, blood pressure lowering at antiallergy.In addition, containing chrysanthemum
The Chinese patent drug and single preparations of ephedrine of flower flavone component are in wide clinical application.
Summary of the invention
The present invention is larger for the drug side-effect for solving the problems, such as existing lipid-loweringing, weight-reducing, and providing one kind has lipid-loweringing, subtracts
The chrysanthemum total flavone and preparation method thereof of fertilizer effect, can effectively reduce blood lipid and weight.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of preparation methods of chrysanthemum total flavone, comprising the following steps:
(1) chrysanthemum is mixed with the ethanol water that mass percent is 70~85%, water-bath obtains extraction mixture；
(2) to being separated by solid-liquid separation after extraction mixture ultrasound, chrysanthemum leaching liquor is obtained；
(3) chrysanthemum leaching liquor is isolated and purified with macroporous absorbent resin, eluting solvent be mass percent 20~
40% ethanol water collects eluent, dry, obtains chrysanthemum total flavone.
Preferably, in step (1), the volume of the ethanol water of the quality and mass percent 70~85% of the chrysanthemum
The ratio between be 1g:28~33mL.
Preferably, in step (1), the temperature of the water-bath is 65~85 DEG C, and the time of water-bath is 20~25min.
Preferably, in step (2), the power of the ultrasound is 150~300w, and ultrasonic extraction time is 15~35min.
Preferably, in step (2), described be separated by solid-liquid separation includes centrifuge separation and vacuum filtration, by what is be centrifugally separating to obtain
Supernatant is filtered by vacuum, and chrysanthemum leaching liquor is obtained.
Preferably, the ratio between the applied sample amount of the macroporous absorbent resin and column volume are 1:8~15.
Preferably, the drying includes concentration and heated-air drying, and the temperature of the heated-air drying is 45~60 DEG C
The present invention provides the chrysanthemum total flavones that above-mentioned technical proposal the method is prepared.
The present invention provides chrysanthemum total flavones described in above-mentioned technical proposal to prepare the application in fat-reducing medicament.
The present invention provides chrysanthemum total flavones described in preceding solution to prepare the application in slimming medicine.
It is that the present invention obtains the utility model has the advantages that
The present invention provides a kind of preparation method of chrysanthemum total flavone, the second for being 70~85% by chrysanthemum and mass percent
Alcohol solution mixing, water-bath obtain extraction mixture；To being separated by solid-liquid separation after extraction mixture ultrasound, chrysanthemum leaching liquor is obtained；With
Macroporous absorbent resin isolates and purifies chrysanthemum leaching liquor, and eluting solvent is that the ethyl alcohol of mass percent 20~40% is water-soluble
Liquid collects eluent, dry, obtains chrysanthemum total flavone.The present invention improves the extraction of effective component using ultrasonic extraction auxiliary
Rate, the chrysanthemum total flavone purity being prepared can reach 84.5%, and the recovery rate of chrysanthemum total flavone can reach 5~7%.
The embodiment of the present invention experiments have shown that, can reduce using the chrysanthemum total flavone that the method for the invention is prepared
Rat blood serum TG, TC, LDL-C, NEFA of induction high in fat are horizontal, and elevating HDL-C is horizontal, reduce blood lipid and weight, improve
The effect of sex factor is protected, lipids disorder status can be improved by influencing relevant enzyme (LPL) of lipid metaboli etc., promote lipid
Remarkable effect has all been played in the approach such as decomposition；It is seen on histopathology morphology, chrysanthemum total flavone can mitigate caused liver high in fat
Popular name for becomes, and reduces the accumulation of lipid in liver.Therefore, chrysanthemum flavones has apparent weight-reducing and effect for reducing fat.
Detailed description of the invention
Fig. 1 is the rat liver pathological section in embodiment 2 after different group administrations.
Specific embodiment
The present invention provides a kind of preparation methods of chrysanthemum total flavone, comprising the following steps:
(1) chrysanthemum is mixed with the ethanol water that mass percent is 70~85%, water-bath obtains extraction mixture；
(2) to being separated by solid-liquid separation after extraction mixture ultrasound, chrysanthemum leaching liquor is obtained；
(3) chrysanthemum leaching liquor is isolated and purified with macroporous absorbent resin, eluting solvent be mass percent 20~
40% ethanol water collects eluent, dry, obtains chrysanthemum total flavone.
The present invention mixes chrysanthemum with the ethanol water that mass percent is 70~85%, water-bath, obtains extracting mixing
Object.
In the present invention, the chrysanthemum raw material is preferably according to following criterion picks: big plump, Yu Baise；It is fickle in love compared with
Greatly, yellow；Smell faint scent, mildly bitter flavor；Frostless turk, raw flower, impurity, mildew etc. of damaging by worms.In the present invention, it is used to prepare chrysanthemum
The water content of the chrysanthemum of general flavone is preferably no more than 8%, and the water content of control chrysanthemum raw material is able to extend the shelf-life of raw material
And increase the yield of chrysanthemum total flavone；It is currently preferred using low temperature drying when the water content of chrysanthemum raw material is higher than 8%
Mode reduces the water content of chrysanthemum raw material, and the drying temperature of the low temperature drying is preferably 50~65 DEG C, more preferably 55~60
℃；The drying time of the low temperature drying is preferably 4~6h, more preferably 4.5~5.5h.In the present invention, the chrysanthemum is former
The water content of material preferably uses moisture teller to measure.
In the present invention, the ethanol water with mass percent 70~85% again after the chrysanthemum is preferably crushed
Mixing；Further, the smashed granularity of chrysanthemum is preferably 80~200 mesh, more preferably 100~150 mesh.The present invention is to described
The mode for crushing chrysanthemum is not particularly limited, and is crushed using grinding mode commonly used in the art, such as pulverizer.
In the present invention, the ratio between the volume of ethanol water of the quality with mass percent 70~85% of the chrysanthemum is
1g:28~33mL；More preferably 1g:30~32mL.The currently preferred ethanol water using mass percent 75% into
Row extracts.
In the present invention, the temperature of the water-bath is preferably 65~85 DEG C, and more preferably 70~75 DEG C；The water-bath when
Between preferably 20~25min, more preferably 22~24min.The present invention uses the mode of water-bath to assist heating to accelerate flavones to mention
The process taken improves the yield of flavones.
After obtaining extraction mixture, the present invention obtains chrysanthemum leaching liquor to being separated by solid-liquid separation after extraction mixture ultrasound.In order to
The recovery rate of chrysanthemum total flavone is further increased, the present invention carries out assisted extraction using ultrasonic extraction mode.
In the present invention, the power of the ultrasound is preferably 150~300W, more preferably 200W；The extraction of the ultrasound
Time is preferably 15~35min, more preferably 10~25min.
In the present invention, the separation of solid and liquid preferably includes to be centrifugated and be filtered by vacuum two steps, will be centrifugated
Obtained supernatant is filtered by vacuum, and chrysanthemum leaching liquor is obtained.In the present invention, the revolving speed of the centrifuge separation is preferably
6000~8000r/min；The time of the centrifuge separation is preferably 10~12min.In the present invention, the vacuum filtration is true
Reciprocal of duty cycle is preferably 0.85~0.95MPa, and the purpose of suction filtration is to further remove remaining chrysanthemum particle.
After obtaining chrysanthemum leaching liquor, chrysanthemum leaching liquor is isolated and purified with macroporous absorbent resin, eluting solvent is matter
The ethanol water of percentage 20~40% is measured, eluent is collected, it is dry, obtain chrysanthemum total flavone.
In the present invention, the macroporous absorbent resin is preferably AB-8 macroporous absorbent resin.In the present invention, the elution
Liquid is preferably the ethanol water of mass percent 30%.
In the present invention, the ratio between the applied sample amount of the macroporous absorbent resin and column volume are preferably 1:8~15, more preferably
1:10~12.
In the present invention, the drying includes concentration and heated-air drying；The present invention passes through the side that heated-air drying is first concentrated again
Formula volatilizees moisture in the eluent containing chrysanthemum total flavone completely.In the present invention, the concentration is preferably by the elution
Liquid is concentrated into the 1/5 of original volume；Condensing mode is preferably rotary evaporation.In the present invention, the temperature of the heated-air drying is preferred
It is 45~60 DEG C, more preferably 50~55 DEG C；The drying is volatilized completely to get chrysanthemum total flavone to moisture.Utilize NaNO2-
Al(NO3)3The purity of-NaOH colorimetric method for determining chrysanthemum total flavone can be obtained 84.5% or more, according to the method for the invention system
The recovery rate of standby chrysanthemum total flavone is 5~7%.
The present invention also provides the chrysanthemum total flavones that the preparation method obtains through the above technical solution.
The present invention also provides chrysanthemum total flavones described in above-mentioned technical proposal to prepare the application in fat-reducing medicament.Test table
Bright, rat blood serum TG, TC, LDL-C, NEFA that chrysanthemum total flavone provided by the invention reduces induction high in fat are horizontal, increase serum
HDL-C is horizontal, improves the effect of protection sex factor, can improve disorders of lipid metabolism by influencing relevant enzyme (LPL) of lipid metaboli etc.
State has all played remarkable effect on promoting the approach such as lipidolysis.It is seen on histopathology morphology, chrysanthemum provided by the invention
Flower general flavone can mitigate caused hepatic disease high in fat, reduce the accumulation of lipid in liver.
The present invention also provides chrysanthemum total flavones described in above-mentioned technical proposal to prepare the application in slimming medicine, tests table
Bright, the High fat diet rats model weight after taking chrysanthemum total flavone has significant decrease, shows chrysanthemum total flavone tool provided by the invention
There is significant antiobesity action, can be used in the preparation of slimming medicine.
It is currently preferred, include in the fat-reducing medicament or slimming medicine the chrysanthemum total flavone that is prepared of the present invention and
Pharmaceutically acceptable pharmaceutic adjuvant；The dosage form of the fat-reducing medicament or slimming medicine includes but is not limited to oral solution, tablet, powder
Agent or capsule；The preparation of specific dosage form and the selection of pharmaceutic adjuvant are according to dosage form known in the art.
In the present invention, the chrysanthemum total flavone is as the effective active composition in fat-reducing medicament or slimming medicine, preferably
Can be used as unique effective active composition.In the present invention, in the fat-reducing medicament or slimming medicine, chrysanthemum total flavone purity
Preferably 75~85%, more preferably 80~85%；In the present invention, the amount of application of the chrysanthemum total flavone be preferably 50~
100m g/bw·k g·d。
Technical solution provided by the invention is described in detail below with reference to embodiment, but they cannot be understood
For limiting the scope of the present invention.
Embodiment 1
1, materials and methods
1.1, material and reagent
FLOS CHRYSANTHEMI ALBA from Haizhou of China: Yancheng ocean Ma Zhen；Ethyl alcohol；Sodium hydroxide；Sodium nitrite；Aluminum nitrate；Rutin standard.
1.2, instrument and equipment
Supersonic wave cleaning machine；Ultraviolet-uisible spectrophotometer；Universalpulverizer；Assay balance；Rotary Evaporators；Vacuum is taken out
Filter device；Centrifuge；Drying box；Thermostat water bath etc..
1.3, experimental method
1.3.1, extracting flavonoids process
Chrysanthemum → drying → crushing → be sieved → weighing → impregnate → ultrasound assisted extraction → centrifuge separation → vacuum filtration
→ purifying → rotary evaporation → drying → chrysanthemum total flavone
1.3.2, key points for operation
Chrysanthemum: selection piece is big plump, Yu Baise.It is fickle in love larger, yellow.Smell faint scent, mildly bitter flavor.Frostless turk, raw flower,
Impurity, mildew etc. of damaging by worms.
Drying: utilize moisture teller measurement chrysanthemum raw material original water content, it is desirable that moisture content control 8% with
It is interior, if water content is more than 8%, drying temperature and time is used to be dried: drying temperature for 50~65 DEG C, drying time 4~
6h, until reaching requirement to moisture content.
It crushes: the chrysanthemum after drying being crushed using Universalpulverizer, grinding time 5min, grinding particle size reaches
200 mesh.
Sieving: being sieved to chrysanthemum after crushing using screening plant, and the mesh number of sieve is 200 mesh.
Impregnate: chrysanthemum sample and 75% ethyl alcohol are mixed according to the ratio that solid-liquid ratio is 1g:30mL after weighed sieving, are placed in
20~25min is impregnated in 75 DEG C of tepidariums.
Ultrasound assisted extraction: ultrasonic power 200w, ultrasonic extraction time are 20min.
Centrifuge separation: centrifuge speed 6000-8000r/min, time 10min, by being centrifugally separating to obtain supernatant.
Vacuum filtration: to supernatant, further suction filtration is handled, and vacuum degree is set as 0.9Mpa.
Purifying: chrysanthemum flavones crude extract is isolated and purified using AB-8 macroporous absorbent resin.In applied sample amount and column
The ratio between volume is about 1:10, and 30% ethyl alcohol purified under elution requirement.
Rotary evaporation: Rotary Evaporators are utilized, extracting solution after purification are concentrated under reduced pressure, the temperature of rotary evaporation is
78 DEG C, volume becomes original 1/5 after concentration.
It is dry: use the mode of heated-air drying to be dried (drying temperature is 55 DEG C) concentrate, it is complete to moisture evaporation
Chrysanthemum total flavone is obtained after complete, using the purity of NaNO2-Al (NO3) 3-NaOH colorimetric method for determining chrysanthemum total flavone, can achieve
84.5%, and chrysanthemum total flavone recovery rate can achieve 6%.
Embodiment 2
1, effect of the chrysanthemum flavones to the rat model of induction high in fat
(1) experimental animal and method
50 male SD rats (160~190g) before experiment adaptable fed after a week, fasting 12~14 hours, Yu Chen
Between docking take blood, measurement serum total cholesterol (TC), triglycerides (TG) are simultaneously weighed.It is negative right to be randomly divided into according to TC level
According to group, hyperlipidemia model group, chrysanthemum flavones low dose group, chrysanthemum flavones middle dose group, 5 groups of chrysanthemum flavones high dose group, every group
10.Negative control group is raised with basal feed, remaining each group is raised with high lipid food.
Tested material each group gives chrysanthemum total flavone 25,50,100m g/bwk gd that embodiment 1 is prepared respectively
Stomach-filling, negative control group and hyperlipidemia model group give distilled water stomach-filling.Groups of animals is freely ingested drinking-water, continuous to feed 8 weeks.
(2) observation index
Observation animal is primary daily, and observation animal mental status, behavior, hair color have without exception, weighs the weight of animals weekly
And record, understand the weight of animals growth pattern；Blood lipids: total cholesterol (TC), triglycerides (TG), high density lipoprotein level
White cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), free fatty acid (NEFA), lipoprotein lipase (LPL)；Liver
Dirty hE pathological section.
2, test result
(1) influence of the chrysanthemum total flavone to rat ordinary circumstance
1 chrysanthemum total flavone of table to rat body weight (g) influence (N=10)
Note: P < 0.05 compared with negative control group a；P < 0.05 compared with hyperlipidemia model control group b
During entire experiment, animal hair color is normal, has no abnormal behavior.Each group rat body weight is weighed during experiment weekly,
On-test, each group weight differences were without conspicuousness, and hyperlipidemia model group has compared with negative control group weight at the 8th week to the 6th week
Significant difference, there were significant differences (P < 0.05) for tested material middle dose group, high dose group and hyperlipidemia model group, illustrates chrysanthemum total flavone
With apparent antiobesity action, it is shown in Table 1.
(2) influence of the chrysanthemum total flavone to rat blood serum TG
From test the 4th week, with negative control group ratio, the horizontal significant raising of hyperlipidemia model group TG, difference had conspicuousness,
Prompt modeling success.It tests the 6th week and starts, chrysanthemum total flavone tested material each group TG level is below hyperlipidemia model control group, in
Dosage group and high dose group are substantially reduced (P < 0.05), illustrate that chrysanthemum total flavone has apparent effect for reducing fat, are shown in Table 2.
2 chrysanthemum total flavone of table to rat blood serum TG (mmol/L) influence (N=10)
Note: P < 0.05 compared with negative control group a；P < 0.05 compared with hyperlipidemia model control group b
(3) influence of the chrysanthemum flavones to Serum TC
From test the 4th week, with negative control group ratio, the horizontal significant raising (P < 0.05) of hyperlipidemia model group TC, prompt was made
Mould success.It tests the 6th week and starts, chrysanthemum total flavone tested material each group TC level is substantially less than hyperlipidemia model group (P < 0.05),
Illustrate that chrysanthemum total flavone has apparent effect for reducing fat, is shown in Table 3.
3 chrysanthemum total flavone of table to Serum TC (mmol/L) influence (N=10)
Note: P < 0.05 compared with negative control group a；P < 0.05 compared with hyperlipidemia model control group b
(4) influence of the chrysanthemum flavones to rat blood serum HDL-C
From test the 4th week, with negative control group ratio, HDL-C level gradually decreased (P < 0.05)；At the 6th week, chrysanthemum is total
Flavones high dose group HDL-C level is variant compared with hyperlipidemia model group, until when off-test, chrysanthemum total flavone high dose group
HDL-C level variant (P < 0.05) compared with hyperlipidemia model group, is shown in Table 4.HDL is (including dynamic from surrounding tissue by cholesterol
Pulse atherosclerosis patch) it is transported to liver and carries out recycling or drained in the form of cholic acid, this process is known as reverse cholesterol transport,
Just because of hDL participates in reverse cholesterol transport, the effect of lipid-loweringing is played.
4 chrysanthemum total flavone of table to rat blood serum HDL-C (mmol/L) influence (N=10)
Note: P < 0.05 compared with negative control group a；P < 0.05 compared with hyperlipidemia model control group b
(5) influence of the chrysanthemum flavones to rat blood serum LDL-C, NEFA, LPL
Each dosage group LDL-C, NEFA level of chrysanthemum total flavone is significantly lower than hyperlipidemia model group, and LPL activity is apparently higher than height
Rouge model group, is shown in Table 5.
LDL is transformed by VLDL, is the most lipoprotein of blood cholesterol level, when simple hypercholesterolemia,
The raising of serum cholesterol concentration and serum LDL-C level are in parallel relation.LPL is the triglyceride that catalysis is connected with protein
The enzyme of hydrolysis.It is located at the Cavity surface of capillary endothelial, plays a key role in triglyceride metabolism.It can be by blood
In chylomicron and very low density lipoprotein (VLDL) entrained by triglyceride be hydrolyzed into glycerol and fatty acid, be allowed to change
For the fatty acid of small-molecular-weight, for various tissue storages and utilization.
5 chrysanthemum total flavone of table to rat blood serum LDL-C, NEFA, LPL, ApoB influence (N=10)
Note: P < 0.05 compared with negative control group a；P < 0.0 compared with hyperlipidemia model control group b
(4) influence of the chrysanthemum flavones to rat liver Pathomorphologic
6 chrysanthemum total flavone of table is to rat liver pathological change
Hepatic cell fattydegeneration grading: "None" indicates that without obvious lesion, liver cell fat becomes in " slight " lobuli hepatis
Property number account for 25%, " moderate " indicate lobuli hepatis in hepatic cell fattydegeneration number account for 50%, " severe " indicate lobuli hepatis in liver cell
Steatosis number accounts for 75% or more.
As shown in figure 1 and table 1, hepatic pathology has no the results show that without fat drips distribution in negative control group rat hepatocytes
Steatosis and necrosis, without cell infiltration, leaflet structure is complete for portal area；There is fat in hyperlipidemia model group rat hepatocytes
Denaturation, there are cell infiltration in endochylema puffing, lobuli hepatis and portal area；7 severe steatosises of low dose group, intracellular disperse
Property empty balloon-shaped fat drips it is more and big, liver rope is disorganized；4 moderate steatosises of middle dose group have medium tiny fat drips in endochylema；
4 mild fatties of high dose group are denaturalized, and fat drips significantly reduce in endochylema, and sinus hepaticus is more visible, and the arrangement of liver rope is obvious to restore (6 He of table
Fig. 1).
Chi-square Test the results show that each group rat liver occur steatosis number of cases it is variant, hyperlipidemia model group and yin
Property control group have conspicuousness (χ compared to difference2Value is 18.60, P < 0.05), prompt high fat diet to cause hepatic steatosis；
Compared with hyperlipidemia model group, middle and high dosage group can significantly mitigate hepatic steatosis (χ2Value respectively 5.20,9.16, P <
0.05), illustrate that chrysanthemum total flavone has the function of mitigating hepatic steatosis.
3, conclusion
Rat blood serum TG, TC, LDL-C, NEFA that chrysanthemum total flavone can reduce induction high in fat are horizontal, increase serum hdl-
C is horizontal, reduces blood lipid and weight, improves the effect of protection sex factor, can be improved by influencing relevant enzyme (LPL) of lipid metaboli etc.
Lipids disorder status has all played remarkable effect on promoting the approach such as lipidolysis；It is seen on histopathology morphology, chrysanthemum
General flavone can mitigate caused hepatic disease high in fat, reduce the accumulation of lipid in liver.Therefore, chrysanthemum flavones has and significantly subtracts
Fertilizer and effect for reducing fat.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of preparation method of chrysanthemum total flavone, comprising the following steps:
(1) chrysanthemum is mixed, water-bath with the ethanol water that mass percent is 70~85%, obtains extraction mixture；
(2) to being separated by solid-liquid separation after extraction mixture ultrasound, chrysanthemum leaching liquor is obtained；
(3) chrysanthemum leaching liquor is isolated and purified with macroporous absorbent resin, eluting solvent is mass percent 20~40%
Ethanol water collects eluent, dry, obtains chrysanthemum total flavone.
2. preparation method according to claim 1, which is characterized in that in the step (1), the quality and quality hundred of chrysanthemum
Divide than being 1g:28~33mL for 70~85% the ratio between the volume of ethanol water.
3. preparation method according to claim 1 or 2, which is characterized in that in step (1), the temperature of the water-bath is 65
~85 DEG C, the time of water-bath is 20~25min.
4. preparation method according to claim 1, which is characterized in that in step (2), the power of the ultrasound is 150~
300w, ultrasonic time are 15~35min.
5. preparation method according to claim 1 or 4, which is characterized in that in step (2), it is described separation of solid and liquid include from
Heart separation and vacuum filtration, the supernatant being centrifugally separating to obtain is filtered by vacuum, chrysanthemum leaching liquor is obtained.
6. preparation method according to claim 1, which is characterized in that in step (3), the loading of the macroporous absorbent resin
The ratio between amount and column volume are 1:8~15.
7. preparation method according to claim 1 or 6, which is characterized in that in step (3), the drying include concentration and
Heated-air drying, the temperature of the heated-air drying are 45~60 DEG C.
8. a kind of chrysanthemum total flavone that claim 1~7 any one the method is prepared.
9. chrysanthemum total flavone described in claim 8 is preparing the application in fat-reducing medicament.
10. chrysanthemum total flavone described in claim 8 is preparing the application in slimming medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811494945.8A CN109758492A (en) | 2018-12-07 | 2018-12-07 | A kind of chrysanthemum total flavone and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811494945.8A CN109758492A (en) | 2018-12-07 | 2018-12-07 | A kind of chrysanthemum total flavone and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109758492A true CN109758492A (en) | 2019-05-17 |
Family
ID=66451200
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811494945.8A Pending CN109758492A (en) | 2018-12-07 | 2018-12-07 | A kind of chrysanthemum total flavone and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109758492A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116350670A (en) * | 2023-04-12 | 2023-06-30 | 海宁纪亨保健食品有限公司 | Preparation method of chrysanthemum extract |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101167775A (en) * | 2006-10-27 | 2008-04-30 | 郭颂 | Method for extracting chrysanthemum total flavone |
| CN102293806A (en) * | 2011-09-02 | 2011-12-28 | 沈阳药科大学 | Method for extracting total flavonoid active part from Huai chrysanthemum and application of total flavonoid active part |
| CN106038652A (en) * | 2016-06-30 | 2016-10-26 | 卢月莲 | Method for extracting and purifying total flavonoids of chrysanthemum |
| CN107468738A (en) * | 2017-09-22 | 2017-12-15 | 安徽菊泰滁菊草本科技有限公司 | A kind of extracting method of chrysanthemum total flavone |
-
2018
- 2018-12-07 CN CN201811494945.8A patent/CN109758492A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101167775A (en) * | 2006-10-27 | 2008-04-30 | 郭颂 | Method for extracting chrysanthemum total flavone |
| CN102293806A (en) * | 2011-09-02 | 2011-12-28 | 沈阳药科大学 | Method for extracting total flavonoid active part from Huai chrysanthemum and application of total flavonoid active part |
| CN106038652A (en) * | 2016-06-30 | 2016-10-26 | 卢月莲 | Method for extracting and purifying total flavonoids of chrysanthemum |
| CN107468738A (en) * | 2017-09-22 | 2017-12-15 | 安徽菊泰滁菊草本科技有限公司 | A kind of extracting method of chrysanthemum total flavone |
Non-Patent Citations (2)
| Title |
|---|
| 付为琳: ""菊花黄酮的提取纯化及其对脂代谢紊乱大鼠作用的研究"", 《万方平台》 * |
| 王兆丹等: ""响应面法优化菊花黄酮的超声提取工艺研"", 《中国营养学会第十三届全国营养科学大会暨全球华人营养科学家大会论文汇编》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116350670A (en) * | 2023-04-12 | 2023-06-30 | 海宁纪亨保健食品有限公司 | Preparation method of chrysanthemum extract |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105395919B (en) | It is a kind of to contain black fungus extract, the composition with effect for reducing blood fat and preparation method thereof | |
| CN102423352B (en) | Preparation method of Chinese medicinal granules for treating cardio-cerebrovascular diseases | |
| CN102550756A (en) | Compound health-care tea containing vine tea and preparation method thereof | |
| CN106975012B (en) | Traditional Chinese medicine composition for reducing blood sugar and preparation method thereof | |
| CN103417601B (en) | A kind of medical composition and its use with regulating blood lipid action | |
| CN102836188A (en) | Folium microcotis total flavone extract and preparation method and application thereof | |
| CN102526315A (en) | Preparation method of extracts of effective fractions of lychee seeds | |
| CN107875196A (en) | Complex enzyme, extract solution system, medicament, extract and preparation method thereof, application | |
| WO2013155995A1 (en) | Red yeast and kudzu root pharmaceutical composition for regulating blood lipids and preparation method therefor | |
| CN109758492A (en) | A kind of chrysanthemum total flavone and its preparation method and application | |
| CN105963342A (en) | An antiallergic compound flavone composition, and a preparing method and applications thereof | |
| CN103142916B (en) | Medicament for preventing and treating senile dementia and preparation method thereof | |
| CN105726622A (en) | Anti-fatigue traditional Chinese medicine combined extract and its preparation method and use | |
| CN109303800A (en) | A kind of lowering fat and protecting liver composition and its preparation method and application | |
| CN105748652B (en) | Serissa japonica capsule for treating gout and preparation method thereof | |
| CN106535912A (en) | Pharmaceutical composition for controlling blood lipids and body weight, and use thereof | |
| CN109223865A (en) | A kind of preparation method of Folium Mori alkaloid and the application for the Folium Mori alkaloid being prepared | |
| CN104027389B (en) | A kind of antianaphylactic Chinese medicinal compound extract and its preparation method and application | |
| CN109699887B (en) | Peony seed oil soft capsule for reducing blood fat and preparation method thereof | |
| CN103877323B (en) | Medicinal composition for treating non-alcoholic fatty liver disease | |
| CN112716990A (en) | Sunflower disc extract, medicine containing sunflower disc extract, preparation method and application | |
| CN105943681A (en) | Semen litchi steroid saponin extract having function of improving insulin resistance | |
| CN111840411A (en) | Preparation method of lipid-lowering tea | |
| CN110038043A (en) | A kind of pueraria lobata toxin expelling nutrient powder and its preparation method and application | |
| CN110664903A (en) | Composition with blood fat reducing function and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190517 |