CN105395919B - It is a kind of to contain black fungus extract, the composition with effect for reducing blood fat and preparation method thereof - Google Patents

It is a kind of to contain black fungus extract, the composition with effect for reducing blood fat and preparation method thereof Download PDF

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CN105395919B
CN105395919B CN201510816925.8A CN201510816925A CN105395919B CN 105395919 B CN105395919 B CN 105395919B CN 201510816925 A CN201510816925 A CN 201510816925A CN 105395919 B CN105395919 B CN 105395919B
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extract
black fungus
effect
composition
reducing blood
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CN105395919A (en
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孔祥辉
张介驰
马银鹏
刘佳宁
于丽萍
陈鹤
张丕奇
王笑庸
戴肖东
韩增华
马庆芳
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Institute of Microbiology of Heilongjiang Academy of Sciences
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Institute of Microbiology of Heilongjiang Academy of Sciences
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    • A61K36/062—Ascomycota
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/734—Crataegus (hawthorn)
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    • A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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Abstract

The invention discloses a kind of containing black fungus extract, the composition with effect for reducing blood fat and preparation method thereof, and the composition consists of the following compositions: 400~1100mg of Blackfungus polyhexose, 35~100mg of black fungus melanin, 95~350mg of Red Yeast Rice P.E, 75~180mg of ginkgo biloba p.e, 55~170mg of haw thorn extract, 55~170mg of Pollen Tyjphae extract, 70~170mg of licorice.Composition is using a variety of bioactive substances with hypolipemic function such as black fungus extract, Red Yeast Rice P.E, ginkgo biloba p.e, haw thorn extract, Pollen Tyjphae extracts in the present invention, enhance lipid-lowering effect, and the licorice with detoxication and toxicant eliminating function function is increased as composition, have the effect of synergistic, attenuation toxin expelling.There is the effect of apparent concertedness in terms of reducing serum total cholesterol, serum triglyceride and low density lipoprotein cholesterol, have a clear superiority in terms of reducing side effect.

Description

It is a kind of to contain black fungus extract, the composition with effect for reducing blood fat and its preparation Method
Technical field
The present invention relates to a kind of composition and preparation method thereof with effect for reducing blood fat.
Background technique
Hyperlipemia seriously threatens human health.Hyperlipemia can directly result in cerebral infarction, thromboangiitis, obesity The cardiovascular and cerebrovascular diseases such as disease, atherosclerosis, fatty liver, cholelithiasis and atherosclerosis coronary cardiopathy, And cardiovascular and cerebrovascular disease is one of human maximum cause of the death, cardiovascular and cerebrovascular diseases are died of there are about 12,000,000 people every year in the whole world, therefore high in fat The prevention and treatment of mass formed by blood stasis are most important.At present to the treatment of hyperlipidemia based on Western medicine, side effect is big, therefore it is aobvious to develop curative effect It writes, the food aimed at prevention or drug that side effect is low have a very important significance.
Complicated component in compound preparation for reducing blood fat more or less has certain side effect.Therefore developing really has drop blood Rouge effect and the health care product having no toxic side effect or drug have a very important significance and preferable application prospect.
Black fungus is the dietotherapeutic fungi of Eumycota Basidiomycetes Auriculariale Auriculariaceae Auricularia, contains polysaccharide, black The a variety of active ingredients such as element.Traditional medicine is recorded, and black fungus has " mild-natured, sweet in flavor ", " QI invigorating is not hungry, strong will of making light of one's life by commiting suicide ", " clearing lung-heat The effect of moistening lung ", " relieving the five internal organs, toxin expelling gas ".Modern medicine study proves that black fungus, which has, to be improved immunity, anti-aging, resists Cancer, anti-radiation, antithrombus formation, reducing blood lipid, hypoglycemic, promotion digestion function of stomach and intestine and other effects.No matter black fungus is in disease Prevent, treat or daily health caring in terms of, all highlight that its is original, is difficult to many effects substituted.Black fungus has drop blood The active mechanism of rouge is that black fungus can enhance immunity of organisms, adjusts immune function, each organ is normal in maintenance human body Running improves the activity for participating in enzyme, the normal circulation of liver and gallbladder is promoted to act on.Various composition in blood lipid is made to reach normal shape in this way State guarantees human health to reduce the accumulating amount of blood lipid in blood.
The hypolipidemic activity composition Study of black fungus focuses primarily upon polysaccharide at present, and extracts for Blackfungus polyhexose normal The shortcomings that advising aqueous extraction-alcohol precipitation technology is as follows: 1. yield is lower, and higher cost, and Blackfungus polyhexose extract yield is generally 3~ 5%, and mostly laboratory stage, higher cost, it is difficult to amplification production.2. since high temperature leads to the drop blood of effective component when extracting Rouge activity reduces, and limits its extensive use.
Summary of the invention
Contain black fungus extract, the composition with effect for reducing blood fat and its preparation the object of the present invention is to provide a kind of Method, the composition contain black fungus extract, red yeast rice rice extracts, ginkgo biloba p.e, haw thorn extract, cattail pollen and extract Object, licorice have effect for reducing blood fat, and its lipid-lowering effect is significantly better than and black fungus extract, red yeast rice is used alone Extract, ginkgo biloba p.e, haw thorn extract or licorice have the effect of synergistic, Synergistic attenuation.
The purpose of the present invention is what is be achieved through the following technical solutions:
It is a kind of containing black fungus extract, with the composition of effect for reducing blood fat, consist of the following compositions: Blackfungus polyhexose 400~1100mg, 35~100mg of black fungus melanin, 95~350mg of Red Yeast Rice P.E, 75~180mg of ginkgo biloba p.e, 55~170mg of haw thorn extract, 55~170mg of Pollen Tyjphae extract, 70~170mg of licorice.
The preparation method of the above-mentioned composition containing black fungus extract, with effect for reducing blood fat, comprising the following steps:
(1) black fungus is crushed, is stirred evenly with the deionized water of 30~60 times of volumes, place 1~3h, room temperature is using high Pressure differential method (solution is forced into 1.0~1.5Mpa, and rapid discharge is to normal pressure state in 0.1~0.3s) extracts 1~3 time, filtering, Filter vacuum is concentrated under reduced pressure, and dry, pulverize to get Blackfungus polyhexose extract；The sodium hydroxide of 2~5 times of volumes is added in filter residue It is 10.0~11.0 that solution, which adjusts solution ph, stands 1~2h, filtering, it is 3.0~3.5 that supernatant hydrochloric acid, which adjusts pH value, quiet 1~2h is set, is filtered, pellet frozen dry, pulverize, and obtain black fungus melanin.
(2) ginkgo leaf, hawthorn and cattail pollen prepare extract according to following extraction process: raw material crushes, with 10~20 times It is extracted 2~3 times under 200~500W of ethanol solution ultrasonic wave that volume fraction is 50~95%, 0.5~1.0h, merging mention every time Liquid is taken, is filtered, filtrate recycling ethanol, concentration dry, pulverize to get alcohol extracting thing.
(3) the cholamine solution (0.1% ammonium hydroxide, 25% ethyl alcohol) of 6~20 times of volumes is added in Radix Glycyrrhizae in 70~90 DEG C of temperature Degree is lower to carry out 1~2h of refluxing extraction, filters, drying crushes to get licorice.
(4) red yeast rice crushes, using 60~85% ethanol solution of 15~25 times of volumes, under 200~500W of ultrasonic wave It extracts 2~3 times, 0.5~1.0h, combined extract, filtering, filtrate recycling ethanol, concentration dry, pulverize to get alcohol extracting every time Take object.
(5) Blackfungus polyhexose, black fungus melanin, haw thorn extract, ginkgo biloba extract, licorice, cattail pollen are mentioned Take object and Red Yeast Rice P.E to mix to get containing black fungus extract, with the composition of effect for reducing blood fat.
In the present invention, the black fungus raw material carries out autumn in Heilongjiang Province, Ya Buli area, Shangzhi City using black 29 kind The effect of cultivating the black fungus fructification produced, finding the raw material by comparative analysis ingredient such as Blackfungus polyhexose and melanin contain Black fungus of the amount than other area productions is high.
In the present invention, Blackfungus polyhexose content 30~40% (after polysaccharide solution ultrafiltration, is used in the black fungus extract Phenol sulfuric acid procedure measurement), melanin content 3%.
In the present invention, the purity of the Blackfungus polyhexose and melanin is 30~40%.
In the present invention, in the Red Yeast Rice P.E effective component Monacolin-K (Lovastatin) content reach 1.8% with Upper (HPLC method), wherein open loop (acid) Monacolin-K content accounts for 85% or more.
In the present invention, GINKGO BILOBA EXTRACT >=30% (HPLC method), Total Terpene Lactones >=8% in the ginkgo biloba p.e (HPLC method), ginkgoic acid≤3ppm.The assay of total flavonoids is referring to " Chinese Pharmacopoeia " ginkgo biloba p.e method, composition Middle general flavone content uses high-pressure liquid phase chromatogram therapy determining.
In the present invention, 15% or more glycyrrhizic acid content in the licorice, triterpenes and flavone compound always contain Amount 20~30%, Polyhydroxy phenol content 5~35%.
In the present invention, general flavone content 20~25% (HPLC method) in the Pollen Tyjphae extract.
In the present invention, general flavone content 3~25% in the haw thorn extract.
In the present invention, the grinding particle size of the black fungus is 150~240 mesh, 200 mesh of optimal granularity.
In the present invention, the ginkgo leaf, hawthorn, cattail pollen and red yeast rice grinding particle size be 100~150 mesh.
Red yeast rice is generated by Fermentation Condition of Monascus spp, and Fermentation Condition of Monascus spp can produce a variety of metabolites, mainly there is monascorubin, enzyme Class active material, material for lowering blood pressure etc..Applying for Red Yeast Rice P.E is with a long history in China, be used to make wine from ancient times, makes vinegar, is roasting The food colorings such as duck.Modern medicine discovery red yeast rice has the function of reducing cholesterol.Due to playing effect for reducing blood fat in red yeast rice Effective component is not only Monacolin class (Lovastatin), further includes carboxylic acid Lovastatin, ergosterol, pigment, γ- Aminobutyric acid, Dimerumicacid etc. and other compositions, since the other compositions of Red Yeast Rice P.E are also playing its unique work With, thus Red Yeast Rice P.E reducing blood lipid, anticancer and anti-oxidant aspect function and effect may be greater than Lovastatin is applied alone Effect.
Ginkgo leaf is the dried leaf of Ginkgoaceae plant Ginkgo biloba, has that promoting blood circulationization is tired, removes obstruction in channels to relieve pain and other effects, clinically more For cardiovascular and cerebrovascular disease.Up to the present, isolated flavones, terpene lactones, organic acid, amino acid, polysaccharide in ginkgo leaf Equal ingredients.General flavone and terpene lactone have clear bioactivity as the characteristic chemical constituent in ginkgo leaf.
Hawthorn is rosaceous plant, containing various bioactivators, such as triterpenic acid, flavones, wherein flavones ingredient There is decompression, reducing blood lipid, increase the pharmacological actions such as coronary flow, heart tonifying, arrhythmia.According to the pharmacological action of hawthorn, now Have many hawthorn and hawthorn compound preparation is applied to clinic to treat the hearts such as hyperlipemia, coronary heart diseases and angina pectoris, arrhythmia cordis Vascular diseases, China's Hawthorn Resources are abundant, low in cost, there is good value of exploiting and utilizing.
Cattail pollen is the dry pollen of Typhaceae plant or congener, has the function of hemostasis, stagnation resolvation, treating stranguria, for the heart The prevention and treatment of cranial vascular disease.
Licorice has prevention and treatment hyperlipidemia, antiviral, prevention and treatment AIDS, anticancer, enhancing Culture in vitro and subtracts Malicious toxin expelling effect, the main effective ingredient of Radix Glycyrrhizae are glycyrrhizic acid and glucoside member enoxolone.
The present invention has the advantage that
1) Thick many candies extract yield is up to 12~18% or more in black fungus extract, 30% or more purity, and mentions for low temperature It takes, has been sufficiently reserved bioactivity；3% or more melanin recovery rate, 40% or more purity.
2) present invention in composition using black fungus extract, Red Yeast Rice P.E, ginkgo biloba p.e, haw thorn extract, A variety of bioactive substances with hypolipemic function such as Pollen Tyjphae extract, enhance lipid-lowering effect, and increase and have The licorice of detoxication and toxicant eliminating function function has the effect of synergistic, attenuation toxin expelling as composition.Reducing the total gallbladder of serum There is the effects of apparent concertedness in terms of sterol, serum triglyceride and low density lipoprotein cholesterol, are reducing side effect Aspect has a clear superiority.According to reducing blood lipid test operation in the test of national function of health food, the results showed that composition is dropping It is significantly better than in terms of blood lipid effect and black fungus extract, Red Yeast Rice P.E or ginkgo biloba p.e is used alone.
3) black fungus extract extracts separation concentration technique using efficient high-pressure difference low temperature continous way, and whole process uses physics side Formula, low temperature extract, and recovery rate is high and realizes that the ecosystem of active constituent retains.Height pressure difference extractive technique makes cell moment quick-fried Split, come into full contact with solvent, be extracted in black fungus 85% or more polysaccharide, make polysaccharide yield reach 12~ 18%.
4) the composition proves there is auxiliary effect for reducing blood fat through animal experiment and human experiment result, can be applied to it is middle at Medicine, health food or food reduce cholesterol and blood viscosity, promote the microcirculation of cardiovascular and cerebrovascular, improve body fat metabolism, Enhance immunity of organisms, there is auxiliary lipid-lowering function.
Specific embodiment
Below with reference to embodiment, further description of the technical solution of the present invention, and however, it is not limited to this, all right Technical solution of the present invention is modified or replaced equivalently, and without departing from the spirit and scope of the technical solution of the present invention, should all be contained Lid is within the protection scope of the present invention.
Embodiment 1:
It is provided in this embodiment to be consisted of the following compositions containing black fungus extract, the composition with effect for reducing blood fat: Black fungus extract (the melanin 70mg that Blackfungus polyhexose 550mg and purity including purity 40% are 40%), red yeast rice extract Object 330mg, ginkgo biloba p.e 90mg, haw thorn extract 150mg, Pollen Tyjphae extract 150mg, licorice 150mg.
Embodiment 2:
It is provided in this embodiment to be consisted of the following compositions containing black fungus extract, the composition with effect for reducing blood fat: Black fungus extract (the melanin 90mg that Blackfungus polyhexose 1000mg and purity including purity 30% are 40%), red yeast rice extract Object 110mg, ginkgo biloba p.e 160mg, haw thorn extract 80mg, Pollen Tyjphae extract 80mg, licorice 80mg.
Embodiment 3:
It is provided in this embodiment to be consisted of the following compositions containing black fungus extract, the composition with effect for reducing blood fat: Black fungus extract (the melanin 50mg that Blackfungus polyhexose 800mg and purity including purity 40% are 30%), red yeast rice extract Object 250mg, ginkgo biloba p.e 120mg, haw thorn extract 100mg, Pollen Tyjphae extract 100mg, licorice 100mg.This For 1 person-time of consumption per day (1.52g), divides 2 times and take.
Preparation method:
(1) black fungus powder is broken to 200 mesh, is stirred evenly with the deionized water of 40 times of volumes, place 2h, room temperature is using high Pressure differential method (solution is forced into 1.0Mpa, and rapid discharge is to normal pressure state in 0.1s) extracts 2 times, filtering, filter vacuum concentration, It dry, pulverize to get Blackfungus polyhexose extract；The sodium hydroxide solution that 4 times of volumes are added in filter residue adjusts solution ph 10.5,1.5h, filtering are stood, it is 3.2 that supernatant hydrochloric acid, which adjusts pH value, stands 1.5h, filtering, and pellet frozen dry, pulverize, Obtain black fungus melanin.
(2) ginkgo leaf, hawthorn and cattail pollen prepare extract according to following extraction process: raw material is crushed to 100 mesh, with 15 Is extracted 3 times, each 0.5h under the ethanol solution ultrasonic wave 300W that times volume fraction is 60%, combined extract, filtering, filtrate time Ethyl alcohol is received, concentration dry, pulverize to get alcohol extracting thing.
(3) the cholamine solution (0.1% ammonium hydroxide, 25% ethyl alcohol) that 10 times of volumes are added in Radix Glycyrrhizae carries out at a temperature of 80 DEG C Refluxing extraction 1.5h is filtered, and drying crushes to get licorice.
(4) Red Yeast Rice P.E preparation process are as follows: 5 constant virtues organic rice, boiling, cooling, is inoculated with purple leaf Monascus, day at immersion Right cultivation and fermentation, production red yeast rice, are crushed to 100 mesh, using 80% ethanol solution of 20 times of volumes, mention under ultrasonic wave 500W It takes 2 times, each 1.0h, combined extract, filters, filtrate recycling ethanol, concentration dry, pulverize to get alcohol extracting thing.This work The content of the functional red yeast rice effective component of skill production is more clear, and effect is more precisely.It without citrinin, has no toxic side effect, reduces liver Dirty burden, absorption are easier, and effect is more brilliant.
(5) Blackfungus polyhexose, black fungus melanin, haw thorn extract, ginkgo biloba extract, licorice, cattail pollen are mentioned Object and Red Yeast Rice P.E are taken, prepares hard capsule, or be mixed together with pharmaceutic adjuvant and oral preparation, ebonite are made according to a conventional method One of capsule, soft capsule, tablet, granule, powder, pill, tea bag, liquid beverage are a variety of, can be in preparation reducing blood lipid It is applied in drug, health food.
Zoopery:
Experimental method: it is provided using SD rat by Shanghai Slac Experimental Animal Co., Ltd., production licence number For SCXK (Shanghai) 2012-0002；Weight 200-250g, male is random to be grouped, respectively normal group, model group, positive control drug Effects of Xuezhikang 0.2gkg-1Group and black fungus extractive compound preparation 0.135,0.27,0.54gkg-1Basic, normal, high 3 agent Amount group, every group 10.In addition to normal group is given drinking water, each group rat is fed with high lipid food (Nantong Te Luofei feed), 3 times a day, continuous 3 weeks.For model group compared with normal group, TC and LDL-C are significantly raised, there is significant difference (P < 0.01), Show that high lipid food makes the success of Rat Experimental hyperlipidemia animal model.Qualified rat is examined to start to test: gastric infusion, often Day, in addition to Normal group, each group rat continues to feed with high lipid food.Each administration group gives black fungus extractive compound preparation (dosage is respectively 0.135,0.27,0.54gkg to low, middle and high dose groups-1), gastric infusion capacity 20mLkg-1.Positive drug Effects of Xuezhikang 0.2gkg-1, gastric infusion capacity 10mLkg-1.Continuous 8 weeks.The blood sampling of each group rat, separates serum, using glycerol Three esters, total cholesterol, low density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) testing cassete (Beijing Bei Huatai clinical reagent Co., Ltd) each content of measurement.
Experimental result: compared with model group, TG, TC and LDL-C level, difference can be effectively reduced in positive control Effects of Xuezhikang group There is significant (P < 0.01)；Each dosage group serum High Density Lipoprotein Cholesterol (HDL-C) is not significantly lower than model comparison Group；Compound Jew ' s-ear extract low, middle and high dose groups can be substantially reduced TG, TC and LDL-C level, except low dose group reduction is low Except density lipoprotein-cholesterol (LDL-C) difference more significant (P < 0.05), other each groups and model group comparing difference are extremely aobvious It writes (P < 0.01), and each group HDL-C level and model group difference is not significant.Each experimental group blood lipid level testing result such as 1 institute of table Show.
1 black fungus compound preparation of table to hyperlipidemia rats blood lipid level influence ()mmol·L-1
Note: compared with blank groupaP < 0.01,bP<0.05；Compared with model group,cP < 0.01,dP<0.05。
Composition has the function of reducing serum triglyceride, total cholesterol, low-density lipoprotein, and makes high density lipoprotein level White cholesterol slightly improves, and has significant hypolipemic function, and lipid-lowering effect is better than being applied alone any a kind of extraction in composition The lipid-lowering effect (being shown in Table 2) of object.
2 black fungus compound preparation of table and single preparations of ephedrine to hyperlipidemia rats blood lipid level influence () mmol·L-1
Note: compared with blank groupaP < 0.01,bP<0.05；Compared with model group,cP < 0.01,dP<0.05。
Human feeding trial:
To examine therapeutic effect of the invention, 200 hyperlipemic patients are chosen, selected patient meets hyperlipidemia diagnosis Standard.Wherein male 75, women 75, the age 40~63 years old, average age 48 years old.4 groups are randomly divided into, every group of 50 people.The One group is treated with composition prepared by embodiment 1；Second group is treated with composition prepared by embodiment 2；Third group embodiment 3 The composition of preparation is treated；4th group is given placebo for blank control group.Course for the treatment of 90d distinguishes empty stomach 12h before and after treatment TC, TG, LDL-C, HDL-C, the heart, Liver and kidney function and blood, urine, feces routine etc. are measured in early morning venous blood samples afterwards.In experiment opening Chest X-rays, electrocardiogram, abdominal B-scan ultrasonography inspection are carried out before beginning.It the results are shown in Table 3.
Indices determine as follows:
(1) effectively: TC reduces > 10%；TG reduces > 15%；HDL-C rising > 0.104mmol/L.
(2) invalid: not up to effective standard person.
3 human body of table foretastes test group test result (n=50)
Conventional, the Liver and kidney function inspection by the inspection and blood, urine, feces that carry out blood lipids index to each group individual after test Result carry out analysis find each embodiment total effective rate be 96.0% or more, rate of side effects and half a year recurrence rate are 0. Liver and kidney function inspection result shows that test group hepatic and renal function is also more healthy than the physical signs before taking composition, overall performance Composition has the outstanding feature of Synergy and attenuation.
Conclusion:
The present invention has preferable effect to the generation and treatment of hyperlipidemia.
By animal experiments show that: 1) model control group and blank control group compare, serum total cholesterol or low density lipoprotein Protein cholesterol increases, and difference has conspicuousness, and decision model is set up；2) each dosage group is compared with model control group, middle and high dosage Group serum total cholesterol, serum triglyceride, low density lipoprotein cholesterol reduce, and difference has conspicuousness, and each dosage group Serum High Density Lipoprotein Cholesterol is not significantly lower than model control group, shows that the given the test agent auxiliary lipid-lowering function animal is real Test the result positive.
Shown by human feeding trial inspection result: 1) each embodiment total effective rate of composition is 96.0% or more, secondary It reacts incidence and half a year recurrence rate is 0；2) inspection results such as Human Physiology index and Liver and kidney function show composition entirety It is demonstrated by the outstanding feature with Synergy and attenuation.

Claims (5)

1. a kind of preparation method of the composition containing black fungus extract, with effect for reducing blood fat, containing black fungus extract, Composition with effect for reducing blood fat, consists of the following compositions: 400~1100mg of Blackfungus polyhexose, black fungus melanin 35~ 100mg, 95~350mg of Red Yeast Rice P.E, 75~180mg of ginkgo biloba p.e, 55~170mg of haw thorn extract, cattail pollen are extracted 55~170mg of object, 70~170mg of licorice；
It is characterized in that steps are as follows for the preparation method:
(1) black fungus is crushed, is stirred evenly with the deionized water of 30~60 times of volumes, place 1~3h, room temperature uses High Pressure Difference Method is extracted 1~3 time, and filtering, filter vacuum is concentrated under reduced pressure, and dry, pulverize to get Blackfungus polyhexose extract；Filter residue addition 2~ The sodium hydroxide solution adjustment solution ph of 5 times of volumes is 10.0~11.0, stands 1~2h, filtering, supernatant is adjusted with hydrochloric acid PH value is 3.0~3.5, stands 1~2h, filtering, and pellet frozen dry, pulverize, and obtains black fungus melanin；
(2) ginkgo leaf, hawthorn and cattail pollen prepare extract according to following extraction process: raw material crushes, with 10~20 times of volumes 2~3 times are extracted under 200~500W of ethanol solution ultrasonic wave that score is 50~95%, every time 0.5~1.0h, combined extract, Filtering, filtrate recycling ethanol, concentration dry, pulverize to get alcohol extracting thing；
(3) the cholamine solution that 6~20 times of volumes are added in Radix Glycyrrhizae carries out 1~2h of refluxing extraction, mistake at a temperature of 70~90 DEG C Filter, drying crush to get licorice；
(4) red yeast rice crushes, and using 60~85% ethanol solution, extracts 2~3 times under 200~500W of ultrasonic wave, and every time 0.5 ~1.0h, combined extract, filtering, filtrate recycling ethanol, concentration dry, pulverize to get alcohol extracting thing；
(5) by Blackfungus polyhexose, black fungus melanin, haw thorn extract, ginkgo biloba extract, licorice, Pollen Tyjphae extract With Red Yeast Rice P.E mix to get containing black fungus extract, with the composition of effect for reducing blood fat.
2. the preparation method of the composition according to claim 1 containing black fungus extract, with effect for reducing blood fat, It is characterized in that the black fungus is the black fungus fructification that black 29 kind carries out autumn culture.
3. the preparation method of the composition according to claim 1 containing black fungus extract, with effect for reducing blood fat, The purity for being characterized in that the Blackfungus polyhexose and melanin is 30~40%.
4. the preparation method of the composition according to claim 1 containing black fungus extract, with effect for reducing blood fat, The grinding particle size for being characterized in that the black fungus is 150~240 mesh.
5. the preparation method of the composition according to claim 1 containing black fungus extract, with effect for reducing blood fat, The grinding particle size for being characterized in that the ginkgo leaf, hawthorn, cattail pollen and red yeast rice is 100~150 mesh.
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