CN114174518A - 非生物胁迫耐受性植物及方法 - Google Patents

非生物胁迫耐受性植物及方法 Download PDF

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CN114174518A
CN114174518A CN201980098920.2A CN201980098920A CN114174518A CN 114174518 A CN114174518 A CN 114174518A CN 201980098920 A CN201980098920 A CN 201980098920A CN 114174518 A CN114174518 A CN 114174518A
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吕贵华
王国奎
张玉
毛冠凡
王昌贵
陈光武
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Sinobioway Bio Agriculture Group Co Ltd
Pioneer Overseas Corp
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Abstract

分离的多核苷酸和多肽，以及重组DNA构建体可用于赋予增加的耐旱性和产量。本公开发明提供了含有这些重组DNA构建体的组合物(如植物或种子)；以及利用这些重组DNA构建体的方法。该重组DNA构建体包含有多核苷酸可操作地连接一个植物中功能性的启动子，其中所述多核苷酸编码耐旱多肽。

Description

非生物胁迫耐受性植物及方法
技术领域
本发明涉及植物育种和遗传学领域，特别是涉及到提高植物非生物胁迫耐性。
研究背景
生物和非生物原因可以对植物产生胁迫，例如造成生物胁迫的原因包括病原菌感染、昆虫取食、一种植物对另一种植物的寄生，例如槲寄生；非生物胁迫包括诸如有效水分的过量或者不足、极限温度和诸如除草剂的合成化学药品。
非生物胁迫是造成世界范围内农作物减产的主要原因，造成主要农作物平均减产50％以上(Boyer,J.S.(1982)Science 218：443-448；Bray,E.A.等(2000)In Biochemistryand Molecular Biology of Plants,edited by Buchannan,B.B.等,Amer.Soc.PlantBiol.,pp.1158-1249；Mushtaq等,(2018)Journal of Plant Physiology,224-225:156-162)。
因此，需要开发提高植物对非生物胁迫耐受性的成分和方法。本发明提供此类组合物和方法。
发明概述
以下是本发明所涵盖的实施例：
在一个实施例中，本公开发明包括一种分离的多核苷酸，其编码的多肽的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112有至少90％的序列一致性。其中增加植物中所述多核苷酸的表达能提高耐旱性。在某些实施例中，所述分离的多核苷酸编码的氨基酸序列为SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112。在某些实施例中，所述分离的多核苷酸包含的核苷酸序列为SEQ ID NO:1、2、4、5、7、8、10、11、13、14、16、17、19、20、22、23、25、26、28、29、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109或111。在某些实施例中，在干旱条件下增加植物中所述多核苷酸的表达能提高籽粒产量。
本公开发明还提供了一种重组DNA构建体，其包含一种分离的多核苷酸可操作地连接至少一个异源调控元件，其中所述多核苷酸编码的多肽的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112有至少90％的序列一致性。
本公开发明进一步提供了一种改良的植物或种子，其具有编码多肽的至少一个多核苷酸的表达或活性的增加，所述多肽含有的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112有至少90％的序列一致性。在某些实施例中，所述改良的植物或种子在其基因组中包含有一个重组DNA构建体，该重组DNA构建体包含一个多核苷酸可操作地连接至少一个异源调控元件，其中所述多核苷酸编码的多肽的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112有至少90％的序列一致性。在某些实施例中，当与同样生长在干旱条件下的对照植物相比较时，所述改良的植物表现出增加的耐旱性和增加的籽粒产量。
在某些实施例中，所述改良植物或种子在其基因组位点上包含一种靶向基因修饰，该基因组位点含有一种编码多肽的多核苷酸，该多肽的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112有至少90％的序列一致性。其中所述靶向基因修饰增加了多肽的表达和/或活性。在某些实施例中，当与同样生长在干旱条件下的对照植物相比较时，所述改良植物表现出提高的耐旱性和增加的籽粒产量。
在某些实施例中，所述植物选自水稻、玉米、大豆、向日葵、高粱、油菜、小麦、苜蓿、棉花、大麦、小米、甘蔗和柳枝稷。
还提供了增加植物耐旱性的方法，所述方法包括增加编码多肽的至少一个多核苷酸的表达，所述多肽的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112有至少90％的序列一致性。其中，当与对照植物相比较时，所获得的植物表现出增加的耐旱性。
在某些实施例中，增加耐旱性的方法包括：(a)向一个可再生植物细胞引入一个重组DNA构建体，其可操作地连接至少一个异源调控元件，其中所述多核苷酸编码的多肽的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112相比较时有至少80％的序列一致性；和(b)再生所述植物，其中所述植物在其基因组中包含有该重组DNA构建体。
在某些实施例中，增加耐旱性的方法包括：(a)向一个可再生植物细胞的基因组位点上引入一个靶向基因修饰，该基因组位点包含一个编码多肽的多核苷酸，其氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112相比较时有至少80％的序列一致性；和(b)再生所述植物，其中该植物在其基因组中包含有引入的基因修饰和增加的多肽的表达和/或活性。在某些实施例中，靶向基因修饰的引入可使用以下基因组修饰技术：多核苷酸引导的核酸内切酶、CRISPR-Cas核酸内切酶、碱基编辑脱氨酶、锌指核酸酶、转录激活物样效应器核酸酶(TALEN)、工程位点特异性巨核酸酶或Argonaute。在某些实施例中，靶向基因修饰存在于基因组位点的(a)编码区域；(b)非编码区域；(c)调控区域；(d)非翻译区域；或(e)任意(a)-(d)的组合。该靶向基因修饰编码的多肽的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112相比较时有至少80％的一致性。
附图说明和序列表
通过以下详细描述和构成本申请一部分的随附序列列表，可以更全面地理解本发明。此处所附的序列描述和序列列表符合37C.F.R.§§1.821和1.825中规定的专利申请中核苷酸和氨基酸序列披露规则。序列描述包括37C.F.R.§§1.821和1.825中定义的氨基酸的三个字母代码，通过引用并入本文。
表1.序列表详述
Figure BDA0003493421520000031
Figure BDA0003493421520000041
详细描述
本文所述各参考文献全部通过引用并入本文。
如本文和所附权利要求中所使用的，除非上下文另有明确规定，否则单数形式“一个”、“一种”和“所述”包括复数引用。因此，例如，对“植物”的引用包括多个这样的植物；对“细胞”的引用包括本领域技术人员已知的一个或多个细胞及其等效物，等等。
定义
在本文中，植物“增加的耐旱性”是指生理或物理特性的任何可测量的改善，例如产量，当在干旱条件下生长时相对于参照或对照植物测量。通常，当基因组中包含重组DNA构建体或DNA修饰的植物，与基因组中不包含重组DNA构建体或DNA修饰的参照或对照植物相比较时，表现出更强的耐旱性。
“农艺性状”是可测量的指标参数，包括但不限于：叶片绿色程度、籽粒产量、生长速率、总生物量或积累速率、成熟时的鲜重、成熟时的干重、果实产量、种子产量、植物总氮含量、果实氮含量、种子氮含量、植物营养组织氮含量、植物总游离氨基酸含量、果实游离氨基酸含量、种子游离氨基酸含量、植物营养组织游离氨基酸含量、植物总蛋白含量、果实蛋白含量、种子蛋白含量、植物营养组织蛋白质含量、耐旱性、氮的吸收、根的倒伏、收获指数、茎的倒伏、株高、穗高、穗长、耐盐性、分蘖数、圆锥花序的大小、早期苗的活力和低温胁迫下的出苗状况。
“转基因的”指其基因组因异源核酸(如重组DNA构建体)的存在而发生改变的任何细胞、细胞系、愈伤组织、组织、植物部分或植物。例如一个重组DNA构建体，包括最初的转基因事件以及由最初转基因事件产生的有性杂交或无性繁殖产生的事件。本文使用的术语“转基因”不包括通过常规植物育种方法或自然发生的事件(如随机交叉受精、非重组病毒感染、非重组细菌转化、非重组转座)改变基因组(染色体或额外染色体)，或自发突变。
“对照”、“对照植物”或“对照植物细胞”为测定受测试植物或植物细胞的表型变化提供参考，由于转化，受测植物或植物细胞的基因组改变影响到目的基因，测试的植物或植物细胞可以是转基因植物或转基因植物细胞的子代。例如，除了导致测试植物或细胞的遗传改变之外，对照植物可以和测试植物具有相同的遗传背景。
“植物”包括整个植株、植物器官、植物组织、种子和植物细胞以及该植株的子代。植物细胞包括但不限于来自下列物质的细胞：种子、悬浮培养物、胚、分生区域、愈伤组织、叶、根、芽、配子体、孢子体、花粉和小孢子。
“子代”包括植物的任何后续世代。
“修饰植物”包括在其基因组内包含异源多核苷酸或修饰基因或启动子的植物。例如，异源多核苷酸稳定地整合在基因组内，使得该多核苷酸被传递到连续世代。异源多核苷酸可单独或作为重组DNA构建物的一部分整合到基因组中。
关于序列的“异源”意思是来源于外来物种的序列，或者，如果来自同一物种，在组成和/或基因组位置上通过人为干预对其天然的组成形式进行了实质性的修改。
“多核苷酸”、“核酸序列”、“核苷酸序列”或“核酸片段”可互换使用并且是任选含有合成的、非天然的或改变的核苷酸碱基的单链或双链RNA或DNA聚合物。核苷酸(通常以它们的5′-单磷酸形式存在)通过如下它们的单个字母名称来指代：“A”为腺苷酸或脱氧腺苷酸(分别对应RNA或DNA)，“C”表示胞苷酸或脱氧胞苷酸，“G”表示鸟苷酸或脱氧鸟苷酸，“U”表示尿苷酸，“T”表示脱氧胸苷酸，“R”表示嘌呤(A或G)，“Y”表示嘧啶(C或T)，“K”表示G或T，“H”表示A或C或T，“I”表示肌苷，并且“N”表示任何核苷酸。
“多肽”、“肽”、“氨基酸序列”和“蛋白质”在本发明中可互换使用，指氨基酸残基的聚合物。该术语适用于其中一个或多个氨基酸残基是相应的天然存在的氨基酸的人工化学类似物的氨基酸聚合物，以及适用于天然存在的氨基酸聚合物。术语“多肽”、“肽”、“氨基酸序列”和“蛋白质”还可包括修饰形式，包括但不限于糖基化、脂质连接、硫酸盐化、谷氨酸残基的γ羧化、羟化和ADP-核糖基化。
“重组DNA构建体”指在自然界中通常不会一起存在的核酸片段的组合。因此，重组DNA构建体可包含源于不同来源的调控序列和编码序列，或源于相同来源但以不同于通常天然存在的方式排列的调控序列和编码序列。
“调控元件”指位于编码序列的上游(5'非编码序列)、中间或下游(3'非编码序列)，并且影响相关编码序列的转录、RNA加工或稳定性或者翻译的核苷酸序列。调控序列可包括但不限于启动子、翻译前导序列、内含子和多腺苷酸化识别序列。术语“调控序列”和“调控元件”在本文中可互换使用。
“启动子”指能够控制另一核酸片段转录的核酸片段。“植物中有功能启动子”是能够控制植物细胞中基因转录的启动子，无论其是否来源于植物细胞。“组织特异性启动子”和“组织优选启动子”可互换使用，并且指主要但非必须专一地在一种组织或器官中表达，而且也可在一种特定细胞或细胞型中表达的启动子。“发育调控启动子”指其活性由发育事件决定的启动子。
“可操作地连接”指核酸片段连接成单一片段，使得其中一个核酸片段的功能受到另一个核酸片段的调控。例如，在启动子能够调节核酸片段的转录时，该启动子与该核酸片段进行了可操作地连接。
“表达”指功能产物的产生。例如，核酸片段的表达可指核酸片段的转录(如转录生成mRNA或功能RNA)和/或RNA翻译成前体或成熟蛋白质。
在本文中，“增加”、“增多”等是指实验组(例如，具有本文所述DNA修饰的植物)与对照组(例如，不包含DNA修饰的野生型植物)相比的任何可检测到的增加。因此，蛋白质的增加表达包括样品中蛋白质总水平的任何可检测的增加，并且可以使用本领域的常规方法(例如，Western blotting和ELISA)来确定。
在本文中，“产量”指每单位土地收获的农业产量，可包括收获时作物每英亩蒲式耳或每亩千克数，并根据谷物水分进行调整(例如，玉米通常为15％，水稻为13.5％)。谷物水分是在谷物收获时测量的。谷物的调整试验重量确定为每蒲式耳磅或每株克的重量，并根据收获时的谷物水分水平进行调整。
在本文中，在两个多核苷酸或多肽序列的上下文中，“序列一致性”或“一致性”指当在指定的比较窗口上对齐以获得最大对应性时相同的两个序列中的残基。当序列同一性百分比用于蛋白质时，人们认识到，不完全相同的残基位置往往因保守的氨基酸替换而不同，氨基酸残基被具有类似化学性质(如电荷或疏水性)的其他氨基酸残基取代，因此不会改变分子的功能性质。当序列在保守替换中不同时，可向上调整序列一致性百分比，以纠正替换的保守性质。通过这种保守替换而不同的序列称为“序列相似性”或“相似性”。进行此调整的方法为本领域技术人员所熟知。通常，这涉及将保守替换作为部分不匹配而不是完全不匹配进行评分，从而增加序列一致性的百分比。因此，例如，如果相同氨基酸的得分为1，非保守替代的得分为0，保守替代的得分为0到1之间。计算保守替换的得分，例如，在PC/GENE(加利福尼亚州山景城Intelligenetics)计划中实施。
“序列一致性百分比(％)”是经过序列比对和引入缺口后，待测序列的序列一致性计算包括，将两个序列中相同核苷酸碱基或氨基酸残基的位置数目，获得匹配位置数，然后将匹配的位置数除以比对窗口中位置总数再乘以100。
除非另有说明，本文提供的序列的多重比对是使用Clustal V比对方法(Higginsand Sharp.(1989)CABIOS,5:151-153)进行的，具有默认参数(间隙惩罚＝10，间隙长度惩罚＝10)。使用Clustal V方法进行成对比对和氨基酸序列同一性百分比计算的默认参数为K倍数＝1，间隙惩罚＝3，窗口＝5，对角线保存＝5。对于核酸，这些参数为K倍数＝2，间隙惩罚＝5，窗口＝4，对角线保存＝4。序列对齐后，使用Clustal V程序，通过查看同一程序上的“序列距离”表，可以获得“一致性百分比”和“散度”值；除非另有说明，否则本文中提供和声明的身份和分歧百分比是以这种方式计算的。
组合物
A.多核苷酸和多肽
本公开发明提供的多核苷酸编码如下多肽：CCP1(含铜蛋白结构域的蛋白)、GPR89A(GPR89A,假定的)、DN-DTP13(表达蛋白)、CTPS1(CTP合成酶，推定)、DN-DTP14(假设蛋白)、LPA1(脂肪酶，推定)、PE1(果胶酯酶，推定)、DN-DTP15(表达蛋白)、DN-DTP16(表达蛋白)，以及CAS2(环青蒿醇合酶，推定)。
一方面本文提供的多核苷酸编码的多肽的氨基酸序列与与SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112等序列中的任何一个都有至少80％的一致性(例如，81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或100％)。
“OsCCP1”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsCCP1多肽(SEQ IDNO:3)由水稻基因位点LOC_Os01g74480.1处的编码序列(CDS)(SEQ ID NO:2)或核苷酸序列(SEQ ID NO:1)编码。在TIGR中的注释为“含铜蛋白结构域的蛋白质，表达”。“CCP1多肽”在本文中是指OsCCP1多肽及其同系物(例如，由SEQ ID NO:53编码的SEQ ID NO:54)或来自其他生物体的同源物，例如玉米(由SEQ ID NO:55编码的SEQ ID NO:56)、高粱(由SEQ ID NO:57编码的SEQ ID NO:58)、拟南芥(由SEQ ID NO:59编码的SEQ ID NO:60)，或大豆(由SEQID NO:61编码的SEQ ID NO:62)。
“OsGPR89A”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsGPR89A多肽(SEQID NO:6)由水稻基因位点LOC_Os04g51180.1处的编码序列(CDS)(SEQ ID NO:5)或核苷酸序列(SEQ ID NO:4)编码。在TIGR中注释为“GPR89A，假定，表达”。“GPR89A多肽”在本文中是指OsGPR89A多肽的同系物或来自其他生物体的同源物，例如玉米(由SEQ ID NO:63编码的SEQ ID NO:64)、高粱(由SEQ ID NO:65编码的SEQ ID NO:66)、拟南芥(由SEQ ID NO:67编码的SEQ ID NO:68)或大豆(由SEQ ID NO:69编码的SEQ ID NO:70)。
“OsDN-DTP13”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsDN-DTP13多肽(SEQ ID NO:9)由水稻基因座LOC_Os09g39360.1处的编码序列(CDS)(SEQ ID NO:8)或核苷酸序列(SEQ ID NO:7)编码。在TIGR中被注释为“表达蛋白”。“DN-DTP13多肽”在本文中是指OsDN-DTP13多肽及其同源物(例如，由SEQ ID NO:71编码的SEQ ID NO:72)或来自其他生物体的同源物。
“OsCTPS1”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsCTPS1多肽(SEQ IDNO:12)由水稻基因位点LOC_Os05g49770.1处的编码序列(CDS)(SEQ ID NO:11)或核苷酸序列(SEQ ID NO:10)编码。在TIGR中注释为“CTP合成酶，推定，表达”。“CTPS1多肽”指OsCTPS1多肽及其同系物(例如，由SEQ ID NO:73编码的SEQ ID NO:74)或来自其他生物体的同源物，例如玉米(由SEQ ID NO:75编码的SEQ ID NO:76)、高粱(由SEQ ID NO:77编码的SEQ IDNO:78)、拟南芥(由SEQ ID NO:79编码的SEQ ID NO:80)，或大豆(由SEQ ID NO:81编码的SEQ ID NO:82)。
“OsDN-DTP14”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsDN-DTP14多肽(SEQ ID NO:15)由水稻基因位点LOC_Os11g14690.1处的编码序列(CDS)(SEQ ID NO:14)或核苷酸序列(SEQ ID NO:13)编码。在TIGR中被注释为“假设蛋白”。“DN-DTP14多肽”在本文中指来自其他生物体的OsDN-DTP14多肽及其同源物或同系物。
“OsLPA1”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsLPA1多肽(SEQ IDNO:18)由水稻基因位点LOC_Os05g28150.1处的编码序列(CDS)(SEQ ID NO:17)或核苷酸序列(SEQ ID NO:16)编码。在TIGR中注释为“脂肪酶，推定，表达”。“LPA1多肽”在本文中指OsLPA1多肽及其同系物(例如，由SEQ ID NO:83编码的SEQ ID NO:84)或来自其他生物体的同源物，例如玉米(由SEQ ID NO:85编码的SEQ ID NO:86)、高粱(由SEQ ID NO:87编码的SEQ ID NO:88)、拟南芥(由SEQ ID NO:89编码的SEQ ID NO:90)，或大豆(由SEQ ID NO:91编码的SEQ ID NO:92)。
“OsPE1”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsPE1多肽(SEQ ID NO:21)由水稻基因座LOC_Os01g19440.1处的编码序列(CDS)(SEQ ID NO:20)或核苷酸序列(SEQ ID NO:19)编码。在TIGR中注释为“果胶酯酶，推测，表达”。“PE1多肽”在本文中是指OsPE1多肽及其同系物(例如，由SEQ ID NO:93编码的SEQ ID NO:94)或来自其他生物体的同源物，例如玉米(由SEQ ID NO:95编码的SEQ ID NO:96)、高粱(由SEQ ID NO:97编码的SEQ ID NO:98)、拟南芥(由SEQ ID NO:99编码的SEQ ID NO:100)，或大豆(由SEQ ID NO:101编码的SEQ ID NO:102)。
“OsDN-DTP15”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsDN-DTP15多肽(SEQ ID NO:24)由水稻基因位点LOC_Os06g13780.1处的编码序列(CDS)(SEQ ID NO:23)或核苷酸序列(SEQ ID NO:22)编码。在TIGR中被注释为“表达蛋白”。“DN-DTP15多肽”在本文中是指OsDN-DTP15多肽及其同系物(例如，由SEQ ID NO:103编码的SEQ ID NO:104)或来自其他生物体的同源物。
“OsDN-DTP16”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsDN-DTP16多肽(SEQ ID NO:27)由水稻基因位点LOC_Os04g20380.1处的编码序列(CDS)(SEQ ID NO:26)或核苷酸序列(SEQ ID NO:25)编码。在TIGR中被注释为“表达蛋白”。“DN-DTP16多肽”在本文中指来自其他生物体的OsDN-DTP16多肽及其同源物或同系物。
“OsCAS2”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsCAS2多肽(SEQ IDNO:30)由水稻基因位点LOC_Os02g04760.1处的编码序列(CDS)(SEQ ID NO:29)或核苷酸序列(SEQ ID NO:28)编码。在TIGR中被注释为“环青蒿醇合酶，推测，表达”。“CAS2多肽”在本文中是指OsCAS2多肽及其同源物(例如，由SEQ ID NO:105编码的SEQ ID NO:106)或来自其他生物体的同源物，例如玉米(由SEQ ID NO:107编码的SEQ ID NO:108)、高粱(由SEQ IDNO:109编码的SEQ ID NO:110)或大豆(由SEQ ID NO:111编码的SEQ ID NO:112)。
应理解，如本领域技术人员将认识到的，本发明包含的不仅仅是特定的示例性序列。核酸片段的改变导致在给定位点产生化学等效的氨基酸，但不影响编码多肽的功能性质，这在本领域是众所周知的。例如，氨基酸丙氨酸(疏水性氨基酸)的密码子可由编码另一疏水性较小的残基(例如甘氨酸)或疏水性较大的残基(例如缬氨酸、亮氨酸或异亮氨酸)的密码子取代。类似地，导致一个带负电的残基替换另一个残基的变化，如用天冬氨酸替换谷氨酸，或一个带正电的残基替换另一个残基，如用赖氨酸替换精氨酸，也可预期产生功能等效的产物。导致多肽分子N端和C端部分改变的核苷酸变化也不会改变多肽的活性。所提议的每一个修饰都在本领域的常规技术范围内，如同确定编码产物的生物活性保留一样
B.重组DNA构建体
本文还提供了包含有本文所描述的任何多核苷酸的重组DNA构建体。在某些实施例中，该重组DNA构建体进一步包含有至少一个调控元件。在某些实施例中，所述的至少一个调控元件是一种异源调控元件。在某些实施例中，重组DNA构建体包含的至少一个调控元件是一种启动子。在某些实施例中，该启动子是一种异源启动子。
许多启动子可以被用于本文中的重组DNA构建体。所述启动子可以依据期望的结果被挑选，且可以包括组成型、组织特异性、诱导型，或其它在宿主器官中表达的启动子。
“组成型”启动子是一种在大多数环境条件下都具有活性的启动子。组成型启动子包括，例如，WO 99/43838和美国专利号6072050中公开的Rsyn7启动子和其他组成型启动子的核心启动子；核心CaMV35S启动子(Odell等,(1985)Nature,313:810-812)；水稻肌动蛋白(McElroy等(1990)Plant Cell,2:163-171)；泛素(Christensen等,(1989)PlantMol.Biol.,12:619-632和Christensen等(1992)Plant Mol.Biol.，18:675-689)；pEMU(Last等(1991)Theor.Appl.Genet..81:581-588)；MAS(Velten等(1984)EMBO J.,3:2723-2730)；ALS启动子(美国专利号5659026)等。其他组成型启动子包括，例如，美国专利号5608149、5608144、5604121、5569597、5466785、5399680、5268463、5608142和6177611。
组织特异性或生长调控型启动子是一种可选择性地调控植物细胞/组织中DNA序列表达的DNA序列，比如在这些对穗发育、种子形成或两者至关重要的细胞/组织中，并且通常将这种DNA序列的表达限制在植物重要的发育期(例如穗发育或种子成熟)。可在本发明的方法中使用引起所需时间和空间表达的任何可识别的启动子。
许多叶片优选启动子在本领域是已知的(Yamamoto等，(1997)Plant J.12(2):255-265；Kwon等，(1994)Plant Physiol.,105:357-367；Yamamoto等，(1994)Plant CellPhysiol.35(5):773-778；Gotor等，(1993)Plant J.3:509-518；Orozco等，(1993)PlantMol.Biol.23(6):1129-1138；和Matsuoka等(1993)Proc.Natl.Acad.Sci.USA,90(20):9586-9590)。
具有种子或胚胎特异性且在本发明中可能有用的启动子包括大豆Kunitz胰蛋白酶抑制剂(Kti3，Jofuku和Goldberg.(1989)，Plant Cell 1:1079-1093)、convicilin、vicilin和豆类(豌豆子叶)(Rerie、W.G.等(1991)Mol.Gene.Genet.，259:149-157；Newbigin、E.J.等(1990)Planta，180:461-470；Higgins，T.J.V.等(1988年)Plant.Mol.Biol.，11:683-695)、玉米醇溶蛋白(玉米胚乳)(Schemthaner、J.P.等,(1988)EMBO J.,7:1249-1255)、菜豆素(豆子叶)(Segupta Gopalan、C.等,(1985)Proc.Natl.Acad.Sci.82:3320-3324)、植物血凝素(豆子叶)(Voelker、T.等,(1987)EMBOJ.,6:3571-3577)，B-伴大豆球蛋白和大豆球蛋白(豆子叶)(Chen、Z-L等,(1988)EMBO J.,7:297-302)，谷蛋白(水稻胚乳)、醇溶蛋白(大麦胚乳)(Marris、C.等,(1988)PlantMol.Biol.,10:359-366)、麦谷蛋白和醇溶蛋白(小麦胚乳)(Colot、V.等,(1987)EMBO J.,6:3559-3564)。与嵌合基因结构中异源编码区可操作连接的种子特异基因的启动子在转基因植物中保持其时空表达模式。这些例子包括在拟南芥和甘蓝型油菜种子中表达脑啡肽的拟南芥2S种子贮藏蛋白基因启动子(Vanderkerckhove等，(1989)Bio/Technology,7:L929-932)、表达荧光素酶的大豆凝集素和大豆β-豆素启动子(Riggs等，(1989)Plant Sci.,63:47-57)，以及表达氯霉素乙酰转移酶的小麦谷蛋白启动子(Colot等,(1987)EMBO J.,6:3559-3564)。
可诱导的启动子选择性表达一个可操作地连接DNA序列，以响应存在的内源或外源刺激，例如通过化合物(化学诱导物)或响应环境、激素、化学和/或发育信号。可诱导或调控的启动子包括，例如，受光、热、压力、洪水或干旱、植物激素、伤害或乙醇、茉莉酸、水杨酸或安全剂等化学物质调节的启动子。
还应考虑到包括一个或多个异源调节元件的组合的合成启动子。
本发明的重组DNA构建体的启动子可以是本领域内已知的任何类型或类别的启动子，这许多启动子中的任何一个都可用于表达本文公开的各种多核苷酸序列，包括期望的多核苷酸序列的天然启动子。用于本发明的重组DNA构建体的启动子能根据期望结果进行挑选。
本发明的重组DNA构建体还可以包括其他调控元件，包括但不限于，翻译先导序列、内含子和多聚腺苷酸化识别序列。在某些实施例中，重组DNA构建体进一步包含一个增强子或沉默子。
一个内含子序列能被添加到5'非翻译区、蛋白编码区或3'非翻译区，以增加在细胞质中累积的成熟信息量。在植物和动物表达结构的转录单元中加入可剪接的内含子已被证明可以在mRNA和蛋白质水平上将基因表达提高1000倍(Buchman和Berg.(1988)Mol.CellBiol.,8:4395-4405；Callis等,(1987)Genes Dev.,1:1183-1200)。
C.植物和植物细胞
提供了一种植物、植物细胞、植物部分、种子和籽粒，在其基因组中含有本文描述的任何重组DNA构建体，使得所述植物、植物细胞、植物部分、种子和/或籽粒具有增加的编码多肽的表达。
还提供了一种植物、植物细胞、植物部分、种子和籽粒，在其基因组位点含有引入的基因修饰，因而能够编码氨基酸序列与选自SEQ ID NOs:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112的序列有至少80％一致性的多肽。在某些实施例中，所述基因修饰提高了编码多肽的活性。在某些实施例中，该基因修饰增加了编码多肽的表达水平。在某些实施例中，所述基因修饰同时提高了编码多肽的表达水平和活性。
所述植物可以是一个单子叶或双子叶植物，例如，水稻、玉米或大豆植物，如玉米杂交植株或玉米自交植株。所述植物还可以是向日葵、高粱、油菜、小麦、苜蓿、棉花、大麦、小米、甘蔗或柳枝稷。
在某些实施例中，当与对照植物相比较时，所述植物表现出增加的耐旱性。在某些实施例中，当与对照植物相比较时，所述植物表现出至少一种农艺性状的改变。
本领域的普通技术人员熟悉用于模拟干旱条件，以及评估经受了模拟或自然发生的干旱条件的植物的耐旱性的方法。例如，可以通过在一段时间内给植物提供低于正常需要的水分或没有水分来模拟干旱条件，也可以通过寻找生理和/或物理条件的差异来评估耐旱性，包括(但不限于)活力、生长、大小或根长，特别是，叶片颜色或叶面积大小。评估耐旱性的其他技术包括测量叶绿素荧光、光合速率和气体交换速率。
D.其它目的性状的堆叠
在一些实施例中，本文公开的发明的多核苷酸可以被设计成分子堆叠。这样，本文中各种宿主细胞、植物、植物细胞、植物部分、种子和/或籽粒能够进一步含有一个或多个目标性状。在某些实施例中，宿主细胞、植物、植物细胞、植物部分、种子和/或籽粒可以堆叠任何目的多核苷酸的组合，以产生具有所需性状组合的植物。在本文中，术语“堆叠”是指包括有多重性状存在于同一个期望植物或器官中。例如，“性状堆叠”可以包含一个分子堆叠，其序列在物理上彼此相邻。在本文中，性状是指源自于特定序列或一组序列的表型。在一个实施例中，分子堆叠包含至少一个赋予草甘膦耐性的多核苷酸。能够赋予草甘膦耐性的多核苷酸在本领域内是已知的。
在某些实施例中，分子堆叠包含至少一个赋予草甘膦耐性的多核苷酸，和至少一个赋予第二除草剂耐性的附加多核苷酸。
在某些实施例中，具有本发明的多核苷酸序列的植物、植物细胞、种子和/或籽粒可以与一个或多个赋予耐性的序列堆叠，这些被赋予耐性的有毒物质如：ALS抑制剂、HPPD抑制剂、2,4-D、其他苯氧基生长素除草剂、芳氧基苯氧基丙酸除草剂、麦草畏、草铵膦除草剂、针对protox酶的除草剂(也称为“protox抑制剂”)。
具有发明的多核苷酸序列的植物、植物细胞、种子和/或籽粒还能与至少一个其它性状进行组合，以产生能进一步包含有多种所需性状组合的植物。例如，具有发明的多核苷酸序列的植物、植物细胞、种子和/或籽粒可以与编码具有杀虫和/或杀虫活性的多核苷酸进行堆叠，或者具有发明的多核苷酸序列的植物、植物细胞、种子和/或籽粒可以与植物抗病毒基因进行组合。
这些堆叠组合能通过任何方法产生，这些方法包括但不限于，通过任何常规方法培育植物，或进行遗传转化。如果所述序列通过基因转化的方法在植物内堆叠，感兴趣的多核苷酸序列能够在任何时间以任何目的进行组合。这些性状能够在共转化过程中与任何转化盒组合提供的相关多核苷酸被同时引入。例如，如果两个序列将被引入，这两个序列可以包含在单独的转化盒(trans)中，也可以包含在同一个转化盒(cis)中。所述序列的表达能被相同的启动子或不同的启动子所驱动。在某些情况下，可以认识到引入一个转化盒将会抑制目的多核苷酸的表达。这可以被结合到任何其他抑制盒或过表达盒的组合中，以产生植物中所需性状的组合。还应该进一步认识到，多核苷酸序列可以通过使用位点特异性重组系统被堆叠在目的基因组位点。例如，参见WO99/25821、WO99/25854、WO99/25840、WO99/25855和WO99/25853，这些专利通过引用并入本文。
方法
提供了一种提高植物耐旱性和/或增加籽粒产量的方法，包括增加至少一个编码多肽的多核苷酸的表达，所述多肽含有的氨基酸序列与SEQ ID NO:3,6,9,12,15,18,21,24,27,30,54,56,58,60,62,64,66,68,70,72,74,76,78,80,82,84,86,88,90,92,94,96,98,100,102,104,106,108,110,或112有至少80％(例如,80％,81％,82％,83％,84％,85％,86％,87％,88％,89％,90％,91％,92％,93％,94％,95％,96％,97％,98％,99％,或100％)的序列一致性。
在某些实施例中，所述方法包括：(a)在可再生植物细胞中表达重组DNA构建体，该重组DNA构建体包含有一个调控元件可操作地连接有编码多肽的多核苷酸；和(b)再生所述植物，其中该植物在其基因组中包含重组DNA构建体。在某些实施例中，所述调控元件是一种异源启动子。
在某些实施例中，所述方法包括：(a)向一个可再生植物细胞的编码所述多肽的基因组位点引入一个靶向基因修饰；和(b)再生所述植物，该植物中编码多肽的表达水平和/或活性是增加的。在某些实施例中，所述靶向基因修饰使用选自以下的基因修饰技术被引入的：多核苷酸引导的核酸内切酶、CRISPR-Cas核酸内切酶、碱基编辑脱氨酶、锌指核酸酶、转录激活物样效应器核酸酶(TALEN)、工程化位点特异性巨核酸酶或Argonaute。在某些实施例中，所述靶向基因修饰存在于基因位点的(a)编码区；(b)非编码区；(c)调控序列；(d)非翻译区；或(e)任何(a)-(d)的组合。所述靶向基因修饰编码的多肽的氨基酸序列与选自SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112的这些序列有至少80％的一致性。
在某些实施例中，所述DNA修饰是在基因组位点插入一个或多个核苷酸(合适的连续的多个核苷酸)。例如，在可操作地连接的基因中插入表达调节元件(EME)，如PCT/US2018/025446中所描述的EME。在某些实施例中，所述靶向DNA修饰可以用本领域内已知的具有高表达的另一些启动子替换内源性多肽启动子。在某些实施例中，靶向DNA修饰可以将本领域内已知的具有高表达的启动子插入到5’UTR，以使内源性多肽的表达受插入的启动子所控制。在某些实施例中，所述DNA修饰是一种优化上文中Kozak序列以增加表达的修饰。在某些实施例中，所述DNA修饰是一种位点上的能够调节表达蛋白的稳定性的多核苷酸修饰或SNP。
使用本发明的方法的植物可以是本文描述的任何植物种类。在某些实施例中，所述植物是玉米、大豆或水稻。
多种方法能够被用于向植物、植物部分、植物细胞、种子和/或籽粒中引入所需的序列。“引入”意指把本发明的多核苷酸或产生的多肽提供给植物、植物细胞、种子和/或谷物，使该序列获得进入植物细胞内部的途径。本文所述的方法不依赖于特定的方法将序列引入植物、植物细胞、种子和/或籽粒，只是单纯的让多核苷酸或多肽能够进入植物至少一个细胞的内部。
转化方法以及将多肽或多核苷酸序列引入植物的方法可能因转化目标植物或植物细胞的类型而不同，即单子叶植物或双子叶植物。将多肽和多核苷酸引入植物细胞的合适方法包括微量注射(Crossway等(1986)Biotechniques，4:320-334)、电穿孔(Riggs等(1986)Proc.Natl.Acad.Sci.USA，83:5602-5606)、农杆菌介导的转化(美国专利号5563055和美国专利号5981840)、直接基因转移(Paszkowski等(1984)EMBO J.3:2717-2722)，和弹道粒子加速(例如，见美国专利号4945050、美国专利号5879918、美国专利号5886244和美国专利号5932782；Tomes等(1995)，in Plant Cell,Tissue,and Organ Culture:Fundamental Methods,编著者Gamborg和Phillips(Springer Verlag,Berlin)；McCabe等(1988)Biotechnology 6:923-926)、和Lec1转化(WO 00/28058)。另见Weissinger等(1988年Ann.Rev.Genet.，22:421-477；Sanford等(1987)Particulate Science andTechnology,5:27-37(洋葱)；Christou等(1988年)Plant Physiol.，87:671-674(大豆)；McCabe等(1988)Bio/Technology 6:923-926(大豆)；Finer和McMullen(1991)In VitroCell Dev.Biol.，27P:175-182(大豆)；Singh等(1998)Theor.Appl.Genet.，96:319-324(大豆)；Datta等(1990)Biotechnology 8:736-740(水稻)；Klein等(1988年)Proc.Natl.Acad.Sci.USA，85:4305-4309(玉米)；Klein等(1988)Biotechnology 6:559-563(玉米)；美国专利号5240855、5322783及5324646；Klein等(1988)Plant Physiol.，91:440-444(玉米)；Fromm等(1990)Biotechnology 8:833-839(玉米)；Hooykaas VanSlogteren等(1984)Nature(London)311:763-764；美国专利号5736369(谷物)；Bytebier等(1987年)Proc.Natl.Acad.Sci.USA，84:5345-5349(百合科)；De Wet等(1985)在胚珠组织的实验操作中，编著者Chapman等(纽约朗文)，第197-209页(花粉)；Kaeppler等(1990)Plant Cell Reports 9:415-418和Kaeppler等(1992)Theor.Appl.Genet.，84:560-566(晶须介导的转化)；D'Halluin等(1992)Plant Cell，4:1495-1505(电穿孔)；Li等(1993)PlantCell Reports，12:250-255和Christou和Ford(1995)Annals of Botany 75:407-413(水稻)；Osjoda等(1996)《Nature Biotechnology 14:745-750(玉米和根癌农杆菌)；所有这些都通过引用并入本文。
在另一些实施例中，本公开发明的多核苷酸可以通过病毒或病毒核酸接触被引入植物中。通常，这些方法涉及到在DNA或RNA分子内结合本发明的核苷酸结构。需要认识到，本发明的多核苷酸序列最初可作为病毒多蛋白的一部分进行人工合成，随后可以在体内或体外通过蛋白质水解处理以产生所需的重组蛋白。进一步，还应认识到本文公开的启动子也包括用于病毒RNA聚合酶转录的启动子。向植物引入多核苷酸并在其中表达编码的蛋白质的方法，涉及到病毒DNA或RNA分子，这在本领域内是已知的。例如，参见美国专利5889191、5889190、5866785、5589367、5316931和Porta等,(1996)MolecularBiotechnology，5:209-221。这些文本通过引用并入本文。
这些转化的细胞可以按照常规方法生长成为植物。参见McCormick等,(1986)Plant Cell Reports,5:81-84。然后可以生长这些植物，并用相同的转化品种或不同的品种授粉，得到的后代具有所需表型特征的组成型表达。可以种植两代或更多代以确定所需表型特征的表达能够稳定保持和遗传，然后收获的种子也能够确保获得所需的表型特征。以这种方式，本发明提供了转化种子(也称为“转基因种子”)，其具有本文公开的多核苷酸，例如，作为表达盒的一部分，稳定地并入其基因组中。
通过植物转化技术获得的前文讨论的这些转化植物细胞，可以培养再生成整个拥有转化表型(即，发明的多核苷酸)的植株，并获得所需的表型，例如增加的产量。关于玉米的转化和再生，参见Gordon-Kamm等,(1990)The Plant Cell,2:603-618。
多种方法可以用于向基因位点引入基因修饰，以在植物、植物部分、植物细胞、种子和/或籽粒中编码本文的多肽。在某些实施例中，所述靶向DNA修饰通过选自以下的基因修饰技术：多核苷酸引导的核酸内切酶、CRISPR-Cas核酸内切酶、碱基编辑脱氨酶、锌指核酸酶、转录激活物样效应器核酸酶(TALEN)、工程化位点特异性巨核酸酶或Argonaute。
在一些实施例中，所述基因修饰可以通过在基因组中靠近所需改变的特定位置诱导双链断裂(DSB)或单链断裂来推进。双链断裂(DSB)的诱导能使用任何双链断裂诱导剂进行，包括但不限于，TALENs、巨核酸酶、锌指核酸酶、Cas9-gRNA系统(基于细菌CRISPR-Cas系统)、引导cpf1核酸内切酶系统，等等。在一些实施例中，双链断裂的引入可以与多核苷酸修饰模板的引入相结合。
多核苷酸修饰模板可以通过任何本领域内已知的方法被引入，比如，但不限于，瞬时导入法、转染、电穿孔、微量注射、颗粒介导的递送、局部递送、晶须介导的递送、通过细胞穿透肽的递送或介孔二氧化硅纳米颗粒(MSN)-介导的直接递送。
所述多核苷酸修饰模板能作为一个单链多核苷酸分子、一个双链多核苷酸分子或作为一个环状DNA(载体DNA)的一部分被引入细胞。该多核苷酸修饰模板还能被束缚在向导RNA和/或Cas内切核酸酶上。
“修饰核苷酸”或“编辑核苷酸”是指与它的不含有修饰核苷酸序列相比较，包含至少一个改变的目的核苷酸序列。这种“改变”包括，例如：(i)至少一个核苷酸的替换，(ii)至少一个核苷酸的删除，(iii)至少一个核苷酸的插入，或(iv)任何(i)-(iii)的组合。
术语“多核苷酸修饰模板”包括一个与被编辑的核苷酸序列相比较时含有至少一个核苷酸修饰的多核苷酸。一个核苷酸修饰能被至少一个核苷酸替代、添加或删除。通常，该多核苷酸修饰模板能进一步包含同源核苷酸序列侧翼至少一个核苷酸修饰，其中该侧翼异源核苷酸序列提供足够的同源物到所需的核苷酸序列用于编辑。
编辑结合双链断裂(DSB)和修饰模板的基因组序列的过程通常包括：提供一个宿主细胞，一个双链断裂(DSB)诱导剂，或一个编码双链断裂(DSB)诱导剂的核酸，以识别染色体序列中的靶向序列并能够诱导基因组序列中的双链断裂(DSB)，以及与被编辑的核苷酸序列相比较，含有至少一个核苷酸改变的至少一个多核苷酸修饰模板。该多核苷酸修饰模板能进一步包含至少一个核苷酸改变的核苷酸序列的侧翼序列，其中侧翼序列与双链断裂(DSB)侧翼的染色体区域基本同源。
核酸内切酶可以通过任何本领域内已知的方法被提供给细胞，例如，但不限于，瞬时导入法、转染、微量注射和/或局部应用或通过重组结构间接应用。核酸内切酶能作为蛋白或作为向导多核苷酸复合物直接提供给细胞或间接通过重组构建体提供。核酸内切酶通过使用本领域内已知的任何方法能短暂地被引入细胞，或能结合到宿主细胞的基因组。在CRISPR-Cas系统的情况下，如2016年5月12日出版的WO2016073433所述，细胞穿透肽(CPP)可促进内切酶和/或引导多核苷酸进入细胞。
除了通过双链断裂技术进行修饰外，还可以使用碱基编辑技术实现一个或多个碱基的无双链断裂修饰，例如，Gaudelli等，(2017)Programmable base editing of A*T toG*C in genomic DNA without DNA cleavage,Nature,551(7681)：464-471；Komor等，(2016)Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage,Nature,533(7603)：420-425。
这些融合包括dCas9或Cas9切口酶以及一个合适的脱氨酶，并且它们可以将，例如胞嘧啶转化为尿嘧啶，而不会导致目标DNA的双链断裂。尿嘧啶然后通过DNA复制或修复转化为胸腺嘧啶。改进的具有靶向灵活性和特异性的碱基编辑器用于编辑内源基因座以产生靶向变异并提高粮食产量。类似地，腺嘌呤碱基编辑器使腺嘌呤转变为肌苷，然后通过修复或复制转化为鸟嘌呤。因此，使用适当的站点特定的基础编辑器，在一个或多个位置进行有针对性的碱基改变，即C·G到T·A转换和A·T到G·C转换。
在一个实施例中，碱基编辑是一种能够直接将目标基因组位点上的一个碱基对转变为另一个，且无需双链断裂(DSB)、同源定向修复(HDR)过程或外部供体DNA模板的基因编辑方法。在一个实施例中，碱基编辑包括(i)一种催化受损的CRISPR–Cas9突变体，其突变使其一个核酸酶结构域不能产生DSB；(ii)单链特异性胞苷/腺嘌呤脱氨酶，在Cas9产生的单链DNA泡中的适当核苷酸窗口内将C转化为U或A转化为G；(iii)尿嘧啶糖基化酶抑制剂(UGI)，阻碍尿嘧啶切除和下游过程，降低碱基编辑效率和产品纯度；(iv)尼克斯酶活性，以切割未编辑的DNA链，然后进行细胞DNA修复过程以替换含G的DNA链。
如本文所用，“基因组区域”是细胞基因组中存在于靶位点任一侧的染色体片段，或者也包括靶位点的一部分。5-10、5-15、5-20、5-25、5-30、5-35、5-40、5-45、5-50、5-55、5-60、5-65、5-70、5-75、5-80、5-85、5-90、5-95、5-100、5-200、5-300、5-400、5-500、5-600、5-700、5-800、5-900、5-1000、5-1100、5-1200、5-1300、5-1400、5-1500、5-1600、5-1700、5-1800、5-1900、5-2000、5-2100、5-2200、5-2300、5-2400、5-2500、5-2600、5-2700、5-2800.5-2900、5-3000、5-3100或更多碱基，使得基因组区域具有足够的同源性以与相应同源区域进行同源重组。
TAL效应核酸酶(TALEN)是一类序列特异性核酸酶，可用于在植物或其他生物体基因组中的特定目标序列上进行双链断裂(Miller等,(2011)Nature Biotechnology,29:143–148)。
核酸内切酶是在多核苷酸链内切割磷酸二酯键的酶。核酸内切酶包括限制性内切酶，其在特定位点切割DNA而不损伤碱基；以及巨核酸酶，也称为归巢内切酶(HEASE)，其与限制性内切酶类似，在特定识别位点结合并切割，但是巨核酸酶的识别位点通常较长，大约18bp或更长(专利申请号PCT/US12/30061，于2012年3月22日提交)。根据保守的序列基序，巨核酸酶被分为四个家族，分别是LAGLIDADG、GIY-YIG、H-N-H和His Cys-box家族。这些基序参与金属离子的配位和磷酸二酯键的水解。HEASE以其长识别位点和耐受DNA底物中的某些序列多态性而著名。巨核酸酶的命名规则与其他限制性内切酶的命名规则相似。对于分别由独立ORF、内含子和内含子编码的酶，巨核酸酶也以前缀F-、I-或PI-为特征。重组过程中的一个步骤涉及多核苷酸在识别位点或其附近的切割。解理活性可用于产生双链断裂。关于位点特异性重组酶及其识别位点的综述，见Sauer(1994)Curr Op Biotechnol 5:521-7；和Sadowski(1993)FASEB 7:760-7。在一些例子中，重组酶来自整合酶或分解酶家族。
锌指核酸酶(ZFNs)是由锌指DNA结合域和双链断裂诱导剂域组成的工程化双链断裂诱导剂。识别位点特异性由锌指结构域赋予，锌指结构域通常包括两个、三个或四个锌指，例如具有C2H2结构，然而其他锌指结构是已知的，并且已经改变基因的结构。锌指结构域适于设计特异性结合一个选定的多核苷酸识别序列的多肽。锌指核酸酶(ZFN)包括连接到非特异性核酸内切酶结构域的工程DNA结合锌指结构域，例如来自IIs型核酸内切酶(例如FokI)的核酸酶结构域。其他功能可以融合到锌指结合域，包括转录激活域、转录抑制域和甲基化酶。在一些实例中，切割活性需要核酸酶结构域的二聚化。每个锌指识别目标DNA中的三个连续碱基对。例如，一个3指结构域识别9个相邻的需要核酸酶二聚化核的苷酸的序列，两组锌指三联体用于结合18个核苷酸的识别序列。
使用双键断裂(DSB)诱导剂(如Cas9-gRNA复合物)进行基因组编辑已有叙述，例如在2015年3月19日发布的美国专利申请US 2015-0082478 A1、2015年2月26日发布的WO2015/026886A1、2016年1月14日发布的WO2016007347和2016年2月18日发布的WO201625131中，所有这些都通过引用并入本文。
实例
以下是本发明某些方面的具体实施例的示例。提供的示例仅用于说明目的，并不打算以任何方式限制本发明的范围。
实例1
耐旱基因的克隆和载体构建
使用含有四个来自花椰菜花叶病毒35S(CaMV 35S)启动子的多聚增强子元件的双元结构，并从四个粳稻品种(中花11、超优1号、台中65和日本晴)开发水稻激活标签群体，按照Lin和Zhang((2005)Plant Cell Rep.23:540-547)所述，通过农杆菌介导的方法转化。建立转基因株系，收获转基因种子，形成水稻激活标签群体。
耐旱标签株系(ATL)经重复的田间试验进行了验证，并确定了T-DNA的插入位点。通过测序法(Zastrow-Hayes G.M.等,(2015)The Plant Genome,8:1-15)确定了T-DNA在ATL株系中的插入位点。克隆该T-DNA左右两侧附近的基因，并通过田间筛选重现该功能基因。此处仅显示了重现功能的基因。依据表2所示的LOC ID和这些基因相应的基因序列，设计引物克隆水稻抗旱基因OsCCP1、OsGPR89A、OsDN-DTP13、OsCTPS1、OsDN-DTP14、OsLPA1、OsPE1、OsDN-DTP15、OsDN-DTP16和OsCAS2。
表2.水稻基因名称、基因ID(来自TIGR)和构建体ID
基因名称 LOC ID 构建体ID
OsCCP1 LOC_Os01g74480.1 DP0927
OsGPR89A LOC_Os04g51180.1 DP1545
OsDN-DTP13 LOC_Os09g39360.1 DP1013
OsCTPS1 LOC_Os05g49770.1 DP2283
OsDN-DTP14 LOC_Os11g14690.1 DP1539
OsLPA1 LOC_Os05g28150.1 DP1408
OsPE1 LOC_Os01g19440.1 DP0921
OsDN-DTP15 LOC_Os06g13780.1 DP1410
OsDN-DTP16 LOC_Os04g20380.1 DP2220
OsCAS2 LOC_Os02g04760.1 DP1110
使用柱试剂盒在琼胶凝脂电泳后提取的PCR扩增产物，然后与TA克隆载体连接。通过测序确认了这些构建体内的序列和方向。每个基因都被克隆到一个植物双元载体中。
实例2
转基因植物株系的转化和基因表达分析
如Lin和Zhang((2005)Plant Cell Rep.23:540-547)所述，通过农杆菌介导的转化，使用实施例1中制备的载体或空载体(DP0158)转化中花11(Oryza sativa L.)。将转化实验室产生的转基因苗(T0)移栽到田间获得T1种子。筛选T1和随后的T2种子以确认转化，并在以下性状筛选中使用阳性鉴定的转基因种子。
通过RT-PCR测定转基因水稻叶片中的基因表达水平。设计引物对过表达的转基因水稻中的OsLPA1基因进行RT-PCR。将ZH11-TC(组织培养ZH11水稻)中的表达水平设置为1.00，并将DP1408转基因水稻植株中的表达水平与ZH11-TC进行比较。基因表达根据EF-1αmRNA水平进行标准化，基因表达分析结果见下表3。
表3.转基因水稻植株中相关表达水平的增长倍数
基因名称 构建体ID 相关表达水平的增长倍数
OsLPA1 DP1408 从49.34到9656.49
实例3
转基因水稻株系的性状
对来自于实例2的转基因水稻株系和ZH11-TC以及DP0158进行抗旱测试。
将来自实施例2的植物的T2种子在32℃下用800ppm多菌灵杀菌8小时，然后洗涤3-5次，在32℃下用水浸泡16小时，之后在35-37℃培养箱中催芽18小时。发芽种子用于以下耐旱性试验。
耐旱性试验-将发芽种子种植在苗床中。在三叶期，将幼苗移栽到试验田，每个转基因株系4个重复，每个重复种植10株，4个重复种植在同一田块中。ZH11-TC和DP0158幼苗位于同一块转基因株系附近，并作为对照进行统计分析。水稻植株按常规使用杀虫剂和化肥进行管理。在穗分化期停止浇水，以便根据天气条件(温度和湿度)在开花期给予干旱胁迫。使用TDR30(Spectrum Technologies，Inc.)在每个区块约10个地点每4天测量一次土壤含水量。在实验过程中观察并记录植物表型。表型包括抽穗期、卷叶程度、干旱敏感性和耐旱性。中午特别注意卷叶程度。在生长季结束时，从每行中间收获每一转基因株系的6株代表性植株，并测量每株的粮食产量。采用混合线性模型对粮食产量数据进行统计分析。
表4提供了这些研究的结果，并提供了每种结构的转基因系的组合数据。
表4.转基因水稻植株的农艺性状
Figure BDA0003493421520000201
Figure BDA0003493421520000211
DP0927转基因水稻植株，两年内在海南田间进行了两次试验。结果一致表明，与对照相比，DP0927转基因水稻在田间干旱条件下的平均单株产量增加。如表4所示，共测试了12个株系，其中两个株系在海南田间表现出良好的结实率。与ZH11-TC和DP0158对照相比，6个株系的产量显著提高(P<0.1)。这12个株系的平均单株产量分别比ZH11-TC和DP0158对照高48％和49％。这些数据表明OsCCP1是一个水稻抗旱基因。
DP1545转基因水稻植株两年内在海南田间进行了两次试验。结果一致表明，与对照相比，DP1545转基因水稻在田间干旱条件下的平均单株产量增加。如表4所示，共测试了10个株系，其中6个株系在海南田间表现出良好的结实率。10个株系的产量显著高于DP0158对照(P<0.1)，6个株系的产量显著高于ZH11-TC对照。这10个株系的平均单株产量分别比ZH11-TC和DP0158对照高16％和76％。这些数据表明OsGPR89A是一个水稻抗旱基因。
DP1013转基因水稻植株两年内在海南田间进行了两次试验。结果一致表明，与对照相比，DP1013转基因水稻在田间干旱条件下的平均单株产量增加。如表4所示，共测试了12个株系，其中3个株系在海南田间表现出良好的结实率。7个株系的产量显著高于DP0158对照(P<0.1)，6个株系的产量显著高于ZH11-TC对照。这12个株系的平均单株产量分别比ZH11-TC和DP0158对照高36％和54％。这些结果表明，与对照相比，OsDN-DTP13转基因水稻植株具有更强的耐旱性。
DP2283转基因水稻植株两年内分别在海南和宁夏田间进行了试验。结果一致表明，与对照相比，DP2283转基因水稻在田间干旱条件下的平均单株产量增加。如表4所示，共测试了8个株系，其中5个株系在宁夏田间表现出良好的结实率。5个株系的产量显著高于DP0158对照(P<0.1)，4个株系的产量显著高于ZH11-TC对照。这8个株系的平均单株产量分别比ZH11-TC和DP0158对照高113％和55％。这些数据表明OsCTPS1是一个水稻抗旱基因。
DP1539转基因水稻植株两年内分别在海南和宁夏田间进行了试验。结果一致表明，与对照相比，在大田干旱条件下，DP1539转基因水稻的平均单株产量增加。如表4所示，共测试了12个株系，其中2个株系在宁夏田间表现出良好的结实率。11个株系的产量显著高于DP0158对照(P<0.1)，7个株系的产量显著高于ZH11-TC对照。这12个株系的平均单株产量分别比ZH11-TC和DP0158对照高50％和73％。这些数据表明OsDN-DTP14是一个水稻抗旱基因。
DP1408转基因水稻植株两年内在海南和宁夏进行了两次试验。结果表明，在大田干旱条件下，DP1408转基因水稻的单株平均产量比对照组有所提高。如表4所示，共测试了8个株系，其中1个株系在海南田间表现出良好的结实率。7个株系的产量显著高于DP0158对照(P<0.1)，1个株系的产量显著高于ZH11-TC对照。这8个株系的平均单株产量分别比ZH11-TC和DP0158对照高38％和64％。这些数据表明OsLPA1是一个水稻抗旱基因。
DP0921转基因水稻植株两年内在海南田间进行了两次试验。结果一致表明，与对照相比，DP0921转基因水稻在大田干旱条件下的平均单株产量增加。如表4所示，共测试了12个株系，其中7个株系的产量显著高于DP0158对照(P<0.1)，6个株系的产量显著高于ZH11-TC对照。这12个株系的平均单株产量分别比ZH11-TC和DP0158对照高55％和53％。这些数据表明OsPE1是一个水稻抗旱基因。
DP1410转基因水稻植株两年内在海南田间进行了两次试验。结果一致表明，与对照相比，在大田干旱条件下，DP1410转基因水稻的平均单株产量增加。如表4所示，在海南田间测试了8个株系，4个株系观察到良好的结实率。7个株系的产量显著高于DP0158对照(P<0.1)，7个株系的产量显著高于ZH11-TC对照。这8个株系的平均单株产量分别比ZH11-TC和DP0158对照高44％和43％。这些数据表明OsDN-DTP15是一个水稻抗旱基因。
DP2220转基因水稻植株两年内在海南田间进行了两次试验。结果一致表明，与对照相比，在大田干旱条件下，DP2220转基因水稻的平均单株产量增加。如表4所示，共测试了8个株系，其中4个株系在海南田间表现出良好的结实率。3个株系的产量显著高于DP0158对照(P<0.1)，3个株系的产量显著高于ZH11-TC对照。这8个株系的平均单株产量分别比ZH11-TC和DP0158对照高51％和35％。这些数据表明OsDN-DTP16是一个水稻抗旱基因。
DP1110转基因水稻植株两年内在海南田间进行了两次试验。结果一致表明，与对照相比，在大田干旱条件下，DP1110转基因水稻的平均单株产量增加。如表4所示，共测试了12个株系，其中5个株系在海南田间表现出良好的结实率。6个株系的产量显著高于DP0158对照(P<0.1)，6个株系的产量显著高于ZH11-TC对照。这12个株系的平均单株产量分别比ZH11-TC和DP0158对照高44％和38％。这些数据表明OsCAS2是一个水稻抗旱基因。
综上所述，这些结果表明，在大田条件下，与对照植株相比，OsCCP1、OsGPR89A、OsDN-DTP13、OsCTPS1、OsDN-DTP14、OsLPA1、OsDN-DTP15、OsDN-DTP16和OsCAS2转基因水稻植株对干旱胁迫的耐受性有所提高。
实例4
水稻耐旱基因在玉米中的转化和评价
玉米植株将通过编码本文所述多肽的一种多核苷酸或来自玉米、拟南芥或其他物种的相应同源物进行转化。该基因在玉米转化载体中的表达可由组成型启动子控制，例如玉米泛素启动子(Christensen等，(1989)Plant Mol.Biol.，12:619-632和Christensen等，(1992)Plant Mol.Biol.，18:675-689)，或在另一启动子的控制下，如应激反应启动子或组织优选启动子。如国际专利出版物WO 2009/006276所述，可通过粒子轰击将重组DNA构建体引入玉米细胞。或者，玉米植株可以通过农杆菌介导的转化用重组DNA构建物转化，基本上如Zhao等在Meth.Mol.Biol.318:315-323(2006)和Zhao等在Mol.Breed.8:323-333(2001)以及1999年11月9日发布的美国专利号5981840中所述。
再生植株的后代，如T1植株，可能受到基于土壤的干旱胁迫。通过图像分析，可以在干旱胁迫之前和期间多次测量植物的面积、体积、生长速率和颜色。与对照相比，在干旱胁迫期间，萎蔫或叶面积减少的显著延迟、黄色积累的减少和/或生长速率的增加将被视为玉米中基因功能增强耐旱性的证据。
实例5
拟南芥中水稻耐旱基因的实验室干旱筛选
为了了解水稻耐旱基因是否能提高双子叶植物的耐旱性或其他性状，本文所述的水稻表达载体可通过农杆菌介导的转化，使用花浸法转化入拟南芥(Columbia)中，并用于鉴定转基因植物(Clough S.T.和Bent A.F.(1998)The Plant Journal 16,735–743；Zhang，X.等(2006)Nature Protocols,1:641-646)。
再生植株的后代，如T1植株，可能受到基于土壤的干旱胁迫。通过图像分析，可以在干旱胁迫之前和期间多次测量植物的面积、体积、生长速率和颜色。与对照相比，干旱胁迫期间枯萎或叶面积减少的显著延迟、黄色积累的减少和/或生长速率的增加将被视为双子叶植物中基因功能增强耐旱性的证据。
序列表
<110> 未名生物农业集团有限公司
先锋海外公司
<120> 非生物胁迫耐受性植物及方法
<130> 2019.07.18
<160> 112
<170> PatentIn version 3.5
<210> 1
<211> 1182
<212> DNA
<213> Oryza sativa
<400> 1
cacttcctag caattaagat catggtgcag cgcacttcat ccgattcggc ggcgtcggct 60
gaggccatgg tcatggacct gagtcccaag cgtcccgcca agtcctacgg cggcgaaggc 120
ggctcgtact ttgattggtc cccctccgag ctgcccatgc tccgcgccgc ctccatcggc 180
gccgccaaac tctcgcttgc cgccggaggc ctcgccctcc ccttctactc cgattcggct 240
aaggtggcct acgtcctcca gggcaagggc acctgcgccg ttctcctccc cgagaccccc 300
tccgagaaga tcctacccat caaagagggc gacgcgctcg cccttccatt cggcgttgtc 360
acctggtggc acaacctgca cgccgccacc accgaactcg tcgtcctctt cctcggggac 420
acatccaagg gccacaccgc cggccgcttc accaacatgc agctcaccgg ctccaccggc 480
atcttcaccg gcttctccac cgagttcgtc gcccgcgcct gggacctccc gcaggacgcc 540
gctgcctccc tcgtctccac ccagcccggc gccggcatcg tgaagctcaa ggatggcttc 600
aggatgcccg agggttgcga caaggacagg gagggcatgg tgctcaactg cttggaggcg 660
ccgctggacg tggacatcaa gaacgggggc cgcgtggtgg tgctgaacac gcagaacctg 720
ccgctggtga aggaggtggg gctgggcgcc gacctggtga ggatcgacgg ccactccatg 780
tgctcgccgg ggttctcgtg cgactcggcg taccaggtga cgtacatcgt gcggggcagc 840
gggcgcgtgc aggtggtggg catcgacggg acgcgcgtgc tggagacccg cgccgagggt 900
ggctgcctct tcatcgtccc caggttcttc gtcgtctcca agatcgccga cgacaccggc 960
atggagtggt tctccatcat caccactccc aaccccatct tctcccacct cgccgggagg 1020
acctccgtct ggaaggccat ctcccccgcc gtgctccagg cctccttcaa caccaccccg 1080
gagatggaga acctcttccg ctccaagagg ctcgactccg agatcttctt cgcccccaat 1140
tcgaattcga tttgatggac aatatccatc ctccatctcc ag 1182
<210> 2
<211> 1134
<212> DNA
<213> Oryza sativa
<400> 2
atggtgcagc gcacttcatc cgattcggcg gcgtcggctg aggccatggt catggacctg 60
agtcccaagc gtcccgccaa gtcctacggc ggcgaaggcg gctcgtactt tgattggtcc 120
ccctccgagc tgcccatgct ccgcgccgcc tccatcggcg ccgccaaact ctcgcttgcc 180
gccggaggcc tcgccctccc cttctactcc gattcggcta aggtggccta cgtcctccag 240
ggcaagggca cctgcgccgt tctcctcccc gagaccccct ccgagaagat cctacccatc 300
aaagagggcg acgcgctcgc ccttccattc ggcgttgtca cctggtggca caacctgcac 360
gccgccacca ccgaactcgt cgtcctcttc ctcggggaca catccaaggg ccacaccgcc 420
ggccgcttca ccaacatgca gctcaccggc tccaccggca tcttcaccgg cttctccacc 480
gagttcgtcg cccgcgcctg ggacctcccg caggacgccg ctgcctccct cgtctccacc 540
cagcccggcg ccggcatcgt gaagctcaag gatggcttca ggatgcccga gggttgcgac 600
aaggacaggg agggcatggt gctcaactgc ttggaggcgc cgctggacgt ggacatcaag 660
aacgggggcc gcgtggtggt gctgaacacg cagaacctgc cgctggtgaa ggaggtgggg 720
ctgggcgccg acctggtgag gatcgacggc cactccatgt gctcgccggg gttctcgtgc 780
gactcggcgt accaggtgac gtacatcgtg cggggcagcg ggcgcgtgca ggtggtgggc 840
atcgacggga cgcgcgtgct ggagacccgc gccgagggtg gctgcctctt catcgtcccc 900
aggttcttcg tcgtctccaa gatcgccgac gacaccggca tggagtggtt ctccatcatc 960
accactccca accccatctt ctcccacctc gccgggagga cctccgtctg gaaggccatc 1020
tcccccgccg tgctccaggc ctccttcaac accaccccgg agatggagaa cctcttccgc 1080
tccaagaggc tcgactccga gatcttcttc gcccccaatt cgaattcgat ttga 1134
<210> 3
<211> 377
<212> PRT
<213> Oryza sativa
<400> 3
Met Val Gln Arg Thr Ser Ser Asp Ser Ala Ala Ser Ala Glu Ala Met
1 5 10 15
Val Met Asp Leu Ser Pro Lys Arg Pro Ala Lys Ser Tyr Gly Gly Glu
20 25 30
Gly Gly Ser Tyr Phe Asp Trp Ser Pro Ser Glu Leu Pro Met Leu Arg
35 40 45
Ala Ala Ser Ile Gly Ala Ala Lys Leu Ser Leu Ala Ala Gly Gly Leu
50 55 60
Ala Leu Pro Phe Tyr Ser Asp Ser Ala Lys Val Ala Tyr Val Leu Gln
65 70 75 80
Gly Lys Gly Thr Cys Ala Val Leu Leu Pro Glu Thr Pro Ser Glu Lys
85 90 95
Ile Leu Pro Ile Lys Glu Gly Asp Ala Leu Ala Leu Pro Phe Gly Val
100 105 110
Val Thr Trp Trp His Asn Leu His Ala Ala Thr Thr Glu Leu Val Val
115 120 125
Leu Phe Leu Gly Asp Thr Ser Lys Gly His Thr Ala Gly Arg Phe Thr
130 135 140
Asn Met Gln Leu Thr Gly Ser Thr Gly Ile Phe Thr Gly Phe Ser Thr
145 150 155 160
Glu Phe Val Ala Arg Ala Trp Asp Leu Pro Gln Asp Ala Ala Ala Ser
165 170 175
Leu Val Ser Thr Gln Pro Gly Ala Gly Ile Val Lys Leu Lys Asp Gly
180 185 190
Phe Arg Met Pro Glu Gly Cys Asp Lys Asp Arg Glu Gly Met Val Leu
195 200 205
Asn Cys Leu Glu Ala Pro Leu Asp Val Asp Ile Lys Asn Gly Gly Arg
210 215 220
Val Val Val Leu Asn Thr Gln Asn Leu Pro Leu Val Lys Glu Val Gly
225 230 235 240
Leu Gly Ala Asp Leu Val Arg Ile Asp Gly His Ser Met Cys Ser Pro
245 250 255
Gly Phe Ser Cys Asp Ser Ala Tyr Gln Val Thr Tyr Ile Val Arg Gly
260 265 270
Ser Gly Arg Val Gln Val Val Gly Ile Asp Gly Thr Arg Val Leu Glu
275 280 285
Thr Arg Ala Glu Gly Gly Cys Leu Phe Ile Val Pro Arg Phe Phe Val
290 295 300
Val Ser Lys Ile Ala Asp Asp Thr Gly Met Glu Trp Phe Ser Ile Ile
305 310 315 320
Thr Thr Pro Asn Pro Ile Phe Ser His Leu Ala Gly Arg Thr Ser Val
325 330 335
Trp Lys Ala Ile Ser Pro Ala Val Leu Gln Ala Ser Phe Asn Thr Thr
340 345 350
Pro Glu Met Glu Asn Leu Phe Arg Ser Lys Arg Leu Asp Ser Glu Ile
355 360 365
Phe Phe Ala Pro Asn Ser Asn Ser Ile
370 375
<210> 4
<211> 1767
<212> DNA
<213> Oryza sativa
<400> 4
tttccccttg cgagatccat ctccggctcc ggcgccggcg cgcgcgcgag gcgtggaggt 60
ggggtggggg aggggggaat gggttggggc gcggtggtgt acgagggcgg cgtggtgggg 120
gcgtcgctgg tggggctcgg ctgggcgggg ctgtggttcc tcaaccggcg gctgtacaag 180
gagtacgagg agcggcgggc gctggtgcag atcctcttcg gcctcgtctt cgccttctcc 240
tgcaacctct tccagctcgt cctcttcgag atcctccccg tcctctccaa gcacgcccgc 300
ttcctcaact ggcacctcga cctcttctgc ctcatcctcc tcctcgtctt cgtcctcccc 360
tactaccact gctacctgct gctgcgcaac tcaggggtgc ggagggagcg ggctctgctc 420
gtcgcggcgc tgttcctgct ggtcttcctc tatggcttct ggcgcatggg gattcatttc 480
cccatgcctt ctccggaaaa agggtttttc acgatgcccc agttggttag taggattggc 540
gtgattggag taagtgtcat ggctgttctt tctggttttg gtgccgtcaa tctgccttac 600
agctatctgt cactgtttat cagggaaatt gatgaaaagg acatcaaaac attggaaagg 660
cagctcatgc aatccatgga gacatgcatc gctaagaaaa agaaaattgt tctgtccaaa 720
atggagatgg agaggattca aggatcagaa gagaagctaa aagccagatc atttctaaag 780
cgtatcgttg ggacagttgt tcgatctgtg caagaagatc aaactgagca ggatatcaaa 840
agcttggatg cagaggtcca ggcactagaa gaactttcca aacaactatt tcttgagata 900
tatgaactcc gtcaagcaaa gatagctgct gcgttttctc gaacttggag aggccatgct 960
cagaatctac taggatatgc tttgtcagtg tattgtgttt ataagatgct caagtccttg 1020
cagagtgttg tctttaaaga ggcaggttct gttgatccag taaccatgac aattaccatc 1080
ttcttgaggc attttgacat tggtattgat gtcactcttt tatcacagta tatatctctc 1140
atatttatcg ggatgttggt tgtcatatct gttcgaggtt tcttggcaaa tgttatgaag 1200
ttcttctttg ctgtttctag agttgggagt ggttcaacaa ccaatgttgt cctttttcta 1260
tctgagatca tgggaatgta cttcatatca tctattcttc tcataaggaa aagcctggca 1320
aatgagtata gggtgatcat tacagatgtt ttgggtggtg atatccagtt tgacttttac 1380
caccgctggt ttgatgctat atttgtggct agtgcatttc tgtccttgct tctgatttct 1440
gcccaataca cctcccggca aacagacaag cacccaattg attgatgctt ttgtaccatt 1500
tgtgcatagt gttcttttaa ccactttgtc caaggttgac ccattcattc taccagtttc 1560
caaaggttgt cgtttctcca tggacttcca tatacagtta cagaacataa tacatcagtt 1620
aacttgaagg tgtctccttg ttttggagta tgtttcctga cgttgaatca tggtgaaaca 1680
gtttctccat atgcaagtca ttaggaatgc tttgcatcgt gatgatgcag aactgcaaat 1740
ggggccggtt gtaatgttgt gtccaca 1767
<210> 5
<211> 1407
<212> DNA
<213> Oryza sativa
<400> 5
atgggttggg gcgcggtggt gtacgagggc ggcgtggtgg gggcgtcgct ggtggggctc 60
ggctgggcgg ggctgtggtt cctcaaccgg cggctgtaca aggagtacga ggagcggcgg 120
gcgctggtgc agatcctctt cggcctcgtc ttcgccttct cctgcaacct cttccagctc 180
gtcctcttcg agatcctccc cgtcctctcc aagcacgccc gcttcctcaa ctggcacctc 240
gacctcttct gcctcatcct cctcctcgtc ttcgtcctcc cctactacca ctgctacctg 300
ctgctgcgca actcaggggt gcggagggag cgggctctgc tcgtcgcggc gctgttcctg 360
ctggtcttcc tctatggctt ctggcgcatg gggattcatt tccccatgcc ttctccggaa 420
aaagggtttt tcacgatgcc ccagttggtt agtaggattg gcgtgattgg agtaagtgtc 480
atggctgttc tttctggttt tggtgccgtc aatctgcctt acagctatct gtcactgttt 540
atcagggaaa ttgatgaaaa ggacatcaaa acattggaaa ggcagctcat gcaatccatg 600
gagacatgca tcgctaagaa aaagaaaatt gttctgtcca aaatggagat ggagaggatt 660
caaggatcag aagagaagct aaaagccaga tcatttctaa agcgtatcgt tgggacagtt 720
gttcgatctg tgcaagaaga tcaaactgag caggatatca aaagcttgga tgcagaggtc 780
caggcactag aagaactttc caaacaacta tttcttgaga tatatgaact ccgtcaagca 840
aagatagctg ctgcgttttc tcgaacttgg agaggccatg ctcagaatct actaggatat 900
gctttgtcag tgtattgtgt ttataagatg ctcaagtcct tgcagagtgt tgtctttaaa 960
gaggcaggtt ctgttgatcc agtaaccatg acaattacca tcttcttgag gcattttgac 1020
attggtattg atgtcactct tttatcacag tatatatctc tcatatttat cgggatgttg 1080
gttgtcatat ctgttcgagg tttcttggca aatgttatga agttcttctt tgctgtttct 1140
agagttggga gtggttcaac aaccaatgtt gtcctttttc tatctgagat catgggaatg 1200
tacttcatat catctattct tctcataagg aaaagcctgg caaatgagta tagggtgatc 1260
attacagatg ttttgggtgg tgatatccag tttgactttt accaccgctg gtttgatgct 1320
atatttgtgg ctagtgcatt tctgtccttg cttctgattt ctgcccaata cacctcccgg 1380
caaacagaca agcacccaat tgattga 1407
<210> 6
<211> 468
<212> PRT
<213> Oryza sativa
<400> 6
Met Gly Trp Gly Ala Val Val Tyr Glu Gly Gly Val Val Gly Ala Ser
1 5 10 15
Leu Val Gly Leu Gly Trp Ala Gly Leu Trp Phe Leu Asn Arg Arg Leu
20 25 30
Tyr Lys Glu Tyr Glu Glu Arg Arg Ala Leu Val Gln Ile Leu Phe Gly
35 40 45
Leu Val Phe Ala Phe Ser Cys Asn Leu Phe Gln Leu Val Leu Phe Glu
50 55 60
Ile Leu Pro Val Leu Ser Lys His Ala Arg Phe Leu Asn Trp His Leu
65 70 75 80
Asp Leu Phe Cys Leu Ile Leu Leu Leu Val Phe Val Leu Pro Tyr Tyr
85 90 95
His Cys Tyr Leu Leu Leu Arg Asn Ser Gly Val Arg Arg Glu Arg Ala
100 105 110
Leu Leu Val Ala Ala Leu Phe Leu Leu Val Phe Leu Tyr Gly Phe Trp
115 120 125
Arg Met Gly Ile His Phe Pro Met Pro Ser Pro Glu Lys Gly Phe Phe
130 135 140
Thr Met Pro Gln Leu Val Ser Arg Ile Gly Val Ile Gly Val Ser Val
145 150 155 160
Met Ala Val Leu Ser Gly Phe Gly Ala Val Asn Leu Pro Tyr Ser Tyr
165 170 175
Leu Ser Leu Phe Ile Arg Glu Ile Asp Glu Lys Asp Ile Lys Thr Leu
180 185 190
Glu Arg Gln Leu Met Gln Ser Met Glu Thr Cys Ile Ala Lys Lys Lys
195 200 205
Lys Ile Val Leu Ser Lys Met Glu Met Glu Arg Ile Gln Gly Ser Glu
210 215 220
Glu Lys Leu Lys Ala Arg Ser Phe Leu Lys Arg Ile Val Gly Thr Val
225 230 235 240
Val Arg Ser Val Gln Glu Asp Gln Thr Glu Gln Asp Ile Lys Ser Leu
245 250 255
Asp Ala Glu Val Gln Ala Leu Glu Glu Leu Ser Lys Gln Leu Phe Leu
260 265 270
Glu Ile Tyr Glu Leu Arg Gln Ala Lys Ile Ala Ala Ala Phe Ser Arg
275 280 285
Thr Trp Arg Gly His Ala Gln Asn Leu Leu Gly Tyr Ala Leu Ser Val
290 295 300
Tyr Cys Val Tyr Lys Met Leu Lys Ser Leu Gln Ser Val Val Phe Lys
305 310 315 320
Glu Ala Gly Ser Val Asp Pro Val Thr Met Thr Ile Thr Ile Phe Leu
325 330 335
Arg His Phe Asp Ile Gly Ile Asp Val Thr Leu Leu Ser Gln Tyr Ile
340 345 350
Ser Leu Ile Phe Ile Gly Met Leu Val Val Ile Ser Val Arg Gly Phe
355 360 365
Leu Ala Asn Val Met Lys Phe Phe Phe Ala Val Ser Arg Val Gly Ser
370 375 380
Gly Ser Thr Thr Asn Val Val Leu Phe Leu Ser Glu Ile Met Gly Met
385 390 395 400
Tyr Phe Ile Ser Ser Ile Leu Leu Ile Arg Lys Ser Leu Ala Asn Glu
405 410 415
Tyr Arg Val Ile Ile Thr Asp Val Leu Gly Gly Asp Ile Gln Phe Asp
420 425 430
Phe Tyr His Arg Trp Phe Asp Ala Ile Phe Val Ala Ser Ala Phe Leu
435 440 445
Ser Leu Leu Leu Ile Ser Ala Gln Tyr Thr Ser Arg Gln Thr Asp Lys
450 455 460
His Pro Ile Asp
465
<210> 7
<211> 496
<212> DNA
<213> Oryza sativa
<400> 7
cacctcgtgt gctacaagtg ctacagtaat ctacatttac taatgacgga ttgattataa 60
tcttttgtgt atgtttttct attataaact cttctattat aaaattttaa tgtgtttctg 120
taaaaaaaaa tccgtatttt tttccatcgt cgtgtcggtt tatttcccac acgacgcaac 180
cgcgctcact cagccaatct acgcattcgc ttacagatac acgcgcgccc agctgaccat 240
ctgcccctta ggctcttggc tgccgcagct ggacaatggc catgcctcac ctgagccaaa 300
tggtctaatg ggtttttctt ttttttatta ttctctgttt tcacctactc actttgatat 360
atcttggtat aaataaactg gatgagatat tatacaatat gtatggtaaa tcccataact 420
ttatcaagcc atgcattttt catgtgacgt ttgtggtgtg tatttttttt ccactgaacc 480
acttttagct actccc 496
<210> 8
<211> 306
<212> DNA
<213> Oryza sativa
<400> 8
atgtttttct attataaact cttctattat aaaattttaa tgtgtttctg taaaaaaaaa 60
tccgtatttt tttccatcgt cgtgtcggtt tatttcccac acgacgcaac cgcgctcact 120
cagccaatct acgcattcgc ttacagatac acgcgcgccc agctgaccat ctgcccctta 180
ggctcttggc tgccgcagct ggacaatggc catgcctcac ctgagccaaa tggtctaatg 240
ggtttttctt ttttttatta ttctctgttt tcacctactc actttgatat atcttggtat 300
aaataa 306
<210> 9
<211> 101
<212> PRT
<213> Oryza sativa
<400> 9
Met Phe Phe Tyr Tyr Lys Leu Phe Tyr Tyr Lys Ile Leu Met Cys Phe
1 5 10 15
Cys Lys Lys Lys Ser Val Phe Phe Ser Ile Val Val Ser Val Tyr Phe
20 25 30
Pro His Asp Ala Thr Ala Leu Thr Gln Pro Ile Tyr Ala Phe Ala Tyr
35 40 45
Arg Tyr Thr Arg Ala Gln Leu Thr Ile Cys Pro Leu Gly Ser Trp Leu
50 55 60
Pro Gln Leu Asp Asn Gly His Ala Ser Pro Glu Pro Asn Gly Leu Met
65 70 75 80
Gly Phe Ser Phe Phe Tyr Tyr Ser Leu Phe Ser Pro Thr His Phe Asp
85 90 95
Ile Ser Trp Tyr Lys
100
<210> 10
<211> 1838
<212> DNA
<213> Oryza sativa
<400> 10
cgatgaagta cgtgctggtg acgggcgggg tggtgagcgg cctcgggaag ggggtgacgg 60
cgagcagcat cggcgtcgtg ctcaaggact gcggcctccg cgtcacctcc atcaagatcg 120
atccttacct caacaccgat gctggaacca tgtctccatt cgagcacggt gaagtgtttg 180
ttttggacga tggtggtgag gtggacttgg accttggaaa ttatgagcga tttctggaca 240
tcaagttgac tcgtgacaac aatataacca cgggaaagat ctatcaggct gttattgaca 300
aggaacgaag aggagactac ttgggaaaaa ctgttcaggt tgtaccacac attacggatg 360
aaatacagga gtggattgaa cgtgtggcaa tgaatccagt tgatggcaca gatgagccag 420
ctgatgtttg tgtaatagaa cttggtggca ctatagggga tattgaatca atgcctttta 480
ttgaagcatt aggtcaattt tcataccgcg taggggctgg aaacttttgc ctagttcatg 540
tcagtcttgt accagttcta aatgtagttg gtgaacagaa aaccaaacct acccaacata 600
gtgtccgtgg actaagagga cttggactga tacctgatat tttagcatgt cgcagtactc 660
agccacttga agaaaatgtg aaagtgaaac tcgcacaatt ttgccacgtt ccgatctcaa 720
atattgtcaa tcttcacgat gttaccaaca tctggcacat ccctttgttg cttagggacc 780
agaaggctca tgaatctatt ctgaaagttt tagatcttca atgtgtggga aaagtgcctc 840
gagcacctaa gctgactgaa tggactgaaa gagccagcaa attcgacaaa ctgaaaactc 900
ctgttaggat tgccatggtt ggaaagtata ctggcctatc agactcctac ttgtcagttt 960
tgaaggctct tctgcatgca tcggttgctt tggacagaaa acttgtggtg gattgggttc 1020
cttcctgtga tcttgaagat tctgcagcca cagagactcc tgatgcatat gaaaaagctt 1080
gggatctact aaagggtgca catggtgtac tagttccagg aggctttgga gatagagggg 1140
tccagggaaa aattcttgct gcaaaatatg cgcgagaaaa caatgttcca tatcttggta 1200
tttgcctggg catgcaaatt gcagtgatcg aattcgcccg ctctgtcatg aagttgcgtg 1260
gtgctaatag tacagagttt gatccagcta caacgacgcc atgtgttatt ttcatgccgg 1320
agggctctaa aacccatatg ggggcaacaa tgcgccttgg atcaaggagg accttcttcc 1380
aggccaatac atgcaaatca gctaagctgt atggcaatgc aagctatgta gatgaaaggc 1440
accgccaccg atatgaggtg aatcctgaaa tggttccaga atttgagaag gcaggtctct 1500
cttttgttgg cagggatgaa agcgggacac gcatggagat aattgagcta ccaactcata 1560
ggttttttgt tggcgcacaa tttcatcctg aattcaagtc aagacctgga aagccatccc 1620
cacttttcat gggattaata gcagcttcat caggacagct tgaccattta cttcagcaat 1680
cttgcggcgt tgtcagctcg ccagtcagac gtggcaacta ctgcaacgga gccacgaaac 1740
aacagaagct ataccaaaat ggacatgtca agaacggcct ggtgaacggc tgctactatg 1800
caaacggcaa cagcattctt catacttagc agcatagc 1838
<210> 11
<211> 1827
<212> DNA
<213> Oryza sativa
<400> 11
atgaagtacg tgctggtgac gggcggggtg gtgagcggcc tcgggaaggg ggtgacggcg 60
agcagcatcg gcgtcgtgct caaggactgc ggcctccgcg tcacctccat caagatcgat 120
ccttacctca acaccgatgc tggaaccatg tctccattcg agcacggtga agtgtttgtt 180
ttggacgatg gtggtgaggt ggacttggac cttggaaatt atgagcgatt tctggacatc 240
aagttgactc gtgacaacaa tataaccacg ggaaagatct atcaggctgt tattgacaag 300
gaacgaagag gagactactt gggaaaaact gttcaggttg taccacacat tacggatgaa 360
atacaggagt ggattgaacg tgtggcaatg aatccagttg atggcacaga tgagccagct 420
gatgtttgtg taatagaact tggtggcact ataggggata ttgaatcaat gccttttatt 480
gaagcattag gtcaattttc ataccgcgta ggggctggaa acttttgcct agttcatgtc 540
agtcttgtac cagttctaaa tgtagttggt gaacagaaaa ccaaacctac ccaacatagt 600
gtccgtggac taagaggact tggactgata cctgatattt tagcatgtcg cagtactcag 660
ccacttgaag aaaatgtgaa agtgaaactc gcacaatttt gccacgttcc gatctcaaat 720
attgtcaatc ttcacgatgt taccaacatc tggcacatcc ctttgttgct tagggaccag 780
aaggctcatg aatctattct gaaagtttta gatcttcaat gtgtgggaaa agtgcctcga 840
gcacctaagc tgactgaatg gactgaaaga gccagcaaat tcgacaaact gaaaactcct 900
gttaggattg ccatggttgg aaagtatact ggcctatcag actcctactt gtcagttttg 960
aaggctcttc tgcatgcatc ggttgctttg gacagaaaac ttgtggtgga ttgggttcct 1020
tcctgtgatc ttgaagattc tgcagccaca gagactcctg atgcatatga aaaagcttgg 1080
gatctactaa agggtgcaca tggtgtacta gttccaggag gctttggaga tagaggggtc 1140
cagggaaaaa ttcttgctgc aaaatatgcg cgagaaaaca atgttccata tcttggtatt 1200
tgcctgggca tgcaaattgc agtgatcgaa ttcgcccgct ctgtcatgaa gttgcgtggt 1260
gctaatagta cagagtttga tccagctaca acgacgccat gtgttatttt catgccggag 1320
ggctctaaaa cccatatggg ggcaacaatg cgccttggat caaggaggac cttcttccag 1380
gccaatacat gcaaatcagc taagctgtat ggcaatgcaa gctatgtaga tgaaaggcac 1440
cgccaccgat atgaggtgaa tcctgaaatg gttccagaat ttgagaaggc aggtctctct 1500
tttgttggca gggatgaaag cgggacacgc atggagataa ttgagctacc aactcatagg 1560
ttttttgttg gcgcacaatt tcatcctgaa ttcaagtcaa gacctggaaa gccatcccca 1620
cttttcatgg gattaatagc agcttcatca ggacagcttg accatttact tcagcaatct 1680
tgcggcgttg tcagctcgcc agtcagacgt ggcaactact gcaacggagc cacgaaacaa 1740
cagaagctat accaaaatgg acatgtcaag aacggcctgg tgaacggctg ctactatgca 1800
aacggcaaca gcattcttca tacttag 1827
<210> 12
<211> 608
<212> PRT
<213> Oryza sativa
<400> 12
Met Lys Tyr Val Leu Val Thr Gly Gly Val Val Ser Gly Leu Gly Lys
1 5 10 15
Gly Val Thr Ala Ser Ser Ile Gly Val Val Leu Lys Asp Cys Gly Leu
20 25 30
Arg Val Thr Ser Ile Lys Ile Asp Pro Tyr Leu Asn Thr Asp Ala Gly
35 40 45
Thr Met Ser Pro Phe Glu His Gly Glu Val Phe Val Leu Asp Asp Gly
50 55 60
Gly Glu Val Asp Leu Asp Leu Gly Asn Tyr Glu Arg Phe Leu Asp Ile
65 70 75 80
Lys Leu Thr Arg Asp Asn Asn Ile Thr Thr Gly Lys Ile Tyr Gln Ala
85 90 95
Val Ile Asp Lys Glu Arg Arg Gly Asp Tyr Leu Gly Lys Thr Val Gln
100 105 110
Val Val Pro His Ile Thr Asp Glu Ile Gln Glu Trp Ile Glu Arg Val
115 120 125
Ala Met Asn Pro Val Asp Gly Thr Asp Glu Pro Ala Asp Val Cys Val
130 135 140
Ile Glu Leu Gly Gly Thr Ile Gly Asp Ile Glu Ser Met Pro Phe Ile
145 150 155 160
Glu Ala Leu Gly Gln Phe Ser Tyr Arg Val Gly Ala Gly Asn Phe Cys
165 170 175
Leu Val His Val Ser Leu Val Pro Val Leu Asn Val Val Gly Glu Gln
180 185 190
Lys Thr Lys Pro Thr Gln His Ser Val Arg Gly Leu Arg Gly Leu Gly
195 200 205
Leu Ile Pro Asp Ile Leu Ala Cys Arg Ser Thr Gln Pro Leu Glu Glu
210 215 220
Asn Val Lys Val Lys Leu Ala Gln Phe Cys His Val Pro Ile Ser Asn
225 230 235 240
Ile Val Asn Leu His Asp Val Thr Asn Ile Trp His Ile Pro Leu Leu
245 250 255
Leu Arg Asp Gln Lys Ala His Glu Ser Ile Leu Lys Val Leu Asp Leu
260 265 270
Gln Cys Val Gly Lys Val Pro Arg Ala Pro Lys Leu Thr Glu Trp Thr
275 280 285
Glu Arg Ala Ser Lys Phe Asp Lys Leu Lys Thr Pro Val Arg Ile Ala
290 295 300
Met Val Gly Lys Tyr Thr Gly Leu Ser Asp Ser Tyr Leu Ser Val Leu
305 310 315 320
Lys Ala Leu Leu His Ala Ser Val Ala Leu Asp Arg Lys Leu Val Val
325 330 335
Asp Trp Val Pro Ser Cys Asp Leu Glu Asp Ser Ala Ala Thr Glu Thr
340 345 350
Pro Asp Ala Tyr Glu Lys Ala Trp Asp Leu Leu Lys Gly Ala His Gly
355 360 365
Val Leu Val Pro Gly Gly Phe Gly Asp Arg Gly Val Gln Gly Lys Ile
370 375 380
Leu Ala Ala Lys Tyr Ala Arg Glu Asn Asn Val Pro Tyr Leu Gly Ile
385 390 395 400
Cys Leu Gly Met Gln Ile Ala Val Ile Glu Phe Ala Arg Ser Val Met
405 410 415
Lys Leu Arg Gly Ala Asn Ser Thr Glu Phe Asp Pro Ala Thr Thr Thr
420 425 430
Pro Cys Val Ile Phe Met Pro Glu Gly Ser Lys Thr His Met Gly Ala
435 440 445
Thr Met Arg Leu Gly Ser Arg Arg Thr Phe Phe Gln Ala Asn Thr Cys
450 455 460
Lys Ser Ala Lys Leu Tyr Gly Asn Ala Ser Tyr Val Asp Glu Arg His
465 470 475 480
Arg His Arg Tyr Glu Val Asn Pro Glu Met Val Pro Glu Phe Glu Lys
485 490 495
Ala Gly Leu Ser Phe Val Gly Arg Asp Glu Ser Gly Thr Arg Met Glu
500 505 510
Ile Ile Glu Leu Pro Thr His Arg Phe Phe Val Gly Ala Gln Phe His
515 520 525
Pro Glu Phe Lys Ser Arg Pro Gly Lys Pro Ser Pro Leu Phe Met Gly
530 535 540
Leu Ile Ala Ala Ser Ser Gly Gln Leu Asp His Leu Leu Gln Gln Ser
545 550 555 560
Cys Gly Val Val Ser Ser Pro Val Arg Arg Gly Asn Tyr Cys Asn Gly
565 570 575
Ala Thr Lys Gln Gln Lys Leu Tyr Gln Asn Gly His Val Lys Asn Gly
580 585 590
Leu Val Asn Gly Cys Tyr Tyr Ala Asn Gly Asn Ser Ile Leu His Thr
595 600 605
<210> 13
<211> 845
<212> DNA
<213> Oryza sativa
<400> 13
cggaggcagt gacaacgaca gcgtggagct aaggcggagg tcggttgggg cgatggttgg 60
cgatggtgat ggctggatgg gtgtgggatt gatccaccgt ccccttgacc atggcggtgg 120
caacggcaag ggcgaggcgg tgcagttcga ccttgaaggt gtgactgcat gcgggaggtc 180
ggtaaggtgc gtatgaggtg gaggccaaca tgtcacgcgg cctgcaggga gtgagaccat 240
ggctggtagt ggaggcggcg gagtgtagcc gtggagtaca cagtggtgga gcaccacgct 300
ggatgcaacc gcgaaccgag tgcgggatgc agcgtgatga caattggcaa ggcgtcggcg 360
tggcggacat gcagcctaag gtggtgcaat ataggcagcc tggccaaggt ggcgaggaag 420
gtggcaccgt gaggtcggat tgagggtctg cgacaggaac ctctcccata cggttgtcct 480
cttgcggcga tagcttgctg ccgaaagatg gccacgttag tgggctagtg cgggggagtg 540
gggatccagc cttctctctc accctctccc tctccaaccc aactgtgtgg ataggggttg 600
agcatgtcgg aggccgtgct cctccttgat gatcagctag tggcgagatg ttggtgtgat 660
gactatgtgt tgaaggtgtt cagttggcaa tggcgacgct tgcggccgcc gcactgccct 720
tagggtgttt ctacggaatc tcactcattc cctgttggat ttccttagtt gaaaaccacg 780
tcatgttgct acgacgaacg gtggtggtgc ttcgttttct tccctggaga cgtcgtttga 840
ggatc 845
<210> 14
<211> 231
<212> DNA
<213> Oryza sativa
<400> 14
atggttggcg atggtgatgg ctggatgggt gtgggattga tccaccgtcc ccttgaccat 60
ggcggtggca acggcaaggg cgaggcggtg cagttcgacc ttgaaggtgt gactgcatgc 120
gggaggtcgg taaggtgttc agttggcaat ggcgacgctt gcggccgccg cactgccctt 180
agggtgtttc tacggaatct cactcattcc ctgttggatt tccttagttg a 231
<210> 15
<211> 76
<212> PRT
<213> Oryza sativa
<400> 15
Met Val Gly Asp Gly Asp Gly Trp Met Gly Val Gly Leu Ile His Arg
1 5 10 15
Pro Leu Asp His Gly Gly Gly Asn Gly Lys Gly Glu Ala Val Gln Phe
20 25 30
Asp Leu Glu Gly Val Thr Ala Cys Gly Arg Ser Val Arg Cys Ser Val
35 40 45
Gly Asn Gly Asp Ala Cys Gly Arg Arg Thr Ala Leu Arg Val Phe Leu
50 55 60
Arg Asn Leu Thr His Ser Leu Leu Asp Phe Leu Ser
65 70 75
<210> 16
<211> 1159
<212> DNA
<213> Oryza sativa
<400> 16
ccttcctcct ctctctctca ctcttgtgac cacgtcggac aagcattccg ctacggcgag 60
atggcgcagg cgacgtacga cgccttcaac cgcgagaagc tctcgccgca tgcgggcctc 120
tcgaggttcg ccatacgccg cttcttcgag tgggcgcagc tgcggggcca cgccgcggcg 180
taccgcgtca ccaggttcct gtacgcgacg tcgtgcgtcg ccgtgccggt aatggccaca 240
acaaggacag tgctcgagat caggtaatgg ccggagtagc gcggcactgt cacctgcaac 300
attgttactg atcgacatgg atgttgccat gcaggtgttg agcgaggtgg cgaggctggt 360
gagcatgtat caggacgagg agctgagcat cacggcgacg ggacacaacc tcggcgccgc 420
actcgcgacg ctgaacgcgt tcgacatcgt cgccaacgga tacaacaggc accccggtca 480
ccgcgttcgt attcgccaac ccgcacgtcg gcgggcacgg cttcaagagt cgcttcgacg 540
gcgcacgtgg ccttggcctc cgcctcctcc gcgtccacaa cgcacgcgac gtcgtcccca 600
ggtactcgac ggcgccgccg aaccgcggcg tgggcaccga gctggcgatc gacacgggcg 660
agtcgccgta cctgagaagg ctcgcgaacg agctggtgtg gcacaaactc gatagctacc 720
tgcacggcgt ggccggcgcg cgcggcggtg aggccgggcg gttcaagctc gccgcgaacg 780
ctggcgaaca aggcctacgg cgcgctgggc gaggagcacg ctcgaaatca acgggcggcg 840
gcgagcgggg ctgagcggcg gccggcggcg tgcgagcgcg cggaagccga cggagcagag 900
cggcggtggg cgagcgcgtg gcagagggcg gagcgaagcg gtggcgcgga gcggcgggca 960
agcgcgcggt ggccgacgga gcggaacggc ggcggtctgc cgccggggga ggagctgggc 1020
gagggcggca gccaccgagc caccgcctcc tctgggcccc gccttcgagc tcctcgccga 1080
cgaccgcggc caccccacct cctccctttc cacgcggtgg tgctcgccgg cgacggtgga 1140
tttggacttg ggaggagct 1159
<210> 17
<211> 645
<212> DNA
<213> Oryza sativa
<400> 17
atggcgcagg cgacgtacga cgccttcaac cgcgagaagc tctcgccgca tgcgggcctc 60
tcgaggttcg ccatacgccg cttcttcgag tgggcgcagc tgcggggcca cgccgcggcg 120
taccgcgtca ccaggttcct gtacgcgacg tcgtgcgtcg ccgtgccggt gttgagcgag 180
gtggcgaggc tggtgagcat gtatcaggac gaggagctga gcatcacggc gacgggacac 240
aacctcggcg ccgcactcgc gacgctgaac gcgttcgaca tcgtcgccaa cggatacaac 300
aggcaccccg gtcaccgcag tcgcttcgac ggcgcacgtg gccttggcct ccgcctcctc 360
cgcgtccaca acgcacgcga cgtcgtcccc aggtactcga cggcgccgcc gaaccgcggc 420
gtgggcaccg agctggcgat cgacacgggc gagtcgccgt acctgagaag gctcgcgaac 480
gagctggtgt ggcacaaact cgatagctac ctgcacggcg tggccggcgc gcgcggcggt 540
gaggccgggc ggttcaagct cgccgcgaac gctggcgaac aaggcctacg gcgcgctggg 600
cgaggagcac gctcgaaatc aacgggcggc ggcgagcggg gctga 645
<210> 18
<211> 214
<212> PRT
<213> Oryza sativa
<400> 18
Met Ala Gln Ala Thr Tyr Asp Ala Phe Asn Arg Glu Lys Leu Ser Pro
1 5 10 15
His Ala Gly Leu Ser Arg Phe Ala Ile Arg Arg Phe Phe Glu Trp Ala
20 25 30
Gln Leu Arg Gly His Ala Ala Ala Tyr Arg Val Thr Arg Phe Leu Tyr
35 40 45
Ala Thr Ser Cys Val Ala Val Pro Val Leu Ser Glu Val Ala Arg Leu
50 55 60
Val Ser Met Tyr Gln Asp Glu Glu Leu Ser Ile Thr Ala Thr Gly His
65 70 75 80
Asn Leu Gly Ala Ala Leu Ala Thr Leu Asn Ala Phe Asp Ile Val Ala
85 90 95
Asn Gly Tyr Asn Arg His Pro Gly His Arg Ser Arg Phe Asp Gly Ala
100 105 110
Arg Gly Leu Gly Leu Arg Leu Leu Arg Val His Asn Ala Arg Asp Val
115 120 125
Val Pro Arg Tyr Ser Thr Ala Pro Pro Asn Arg Gly Val Gly Thr Glu
130 135 140
Leu Ala Ile Asp Thr Gly Glu Ser Pro Tyr Leu Arg Arg Leu Ala Asn
145 150 155 160
Glu Leu Val Trp His Lys Leu Asp Ser Tyr Leu His Gly Val Ala Gly
165 170 175
Ala Arg Gly Gly Glu Ala Gly Arg Phe Lys Leu Ala Ala Asn Ala Gly
180 185 190
Glu Gln Gly Leu Arg Arg Ala Gly Arg Gly Ala Arg Ser Lys Ser Thr
195 200 205
Gly Gly Gly Glu Arg Gly
210
<210> 19
<211> 1759
<212> DNA
<213> Oryza sativa
<400> 19
gatctatcaa gtttgtcagg cgagatgaag caagttctgg cccccataat agccttggtc 60
attggaattg gaacactagc tttcatggcc attagtcctc aggtttgcca tgctgctgca 120
ggaggaagtg ctactgtggc gagaagcatc ttcgtcagca agaagggtag tggtgctgat 180
ttcacaagga tacaagacgc catcaactcc gtcccattcg ccaaccggcg gtggatccgg 240
attcacatcg ccgccggcgt ctacaagtaa cacatgcaat ttcattcttg ttcaaagcat 300
gcatttttcc tcttctgatg tacactactg atgagtctgt gaaattgtgt aagtgattgt 360
taggacctat tcagagtcta acagtgatct tttgatatta aatgagcaat cgttttgatt 420
gatcagaggt gcatggatga cttaaatcaa gacggatttt gtttcgaaat tgggagtgca 480
gaagattttg gtgattatga tttgattgtt tgtgctaatt aattaatttt ggttttgtgt 540
cattgggttt ggaagggaga aggtgagcat accggcgaac aagagcttca tcttgctgga 600
aggcgagggg aggcagcaga cgtcgatcga gtgggcggac cacgccggcg gcggcggcgg 660
ggactccggc acggccgact cgccgacgtt cgcctcgtac gccgccgact tcatggcccg 720
cgacatcacc ttcaaggtac gtacgtcggt gccaccacac gacgacgtac gacgaggtgg 780
tgaccgaccg tgtcgatcgc gccattgctg catgcagaac acgtacggga ggatggcgcc 840
ggcggtggcg gcgctggtgg ccggagaccg gtcggcgttc taccggtgcg gcttcgtggg 900
gctgcaggac acgctgagcg acctgctggg gaggcactac tacgagcgct gctacgtcga 960
gggcgccgtc gacttcatct tcggggaagc ccagtccatc ttccaccgct gccacatctc 1020
caccgcggcg gcggcggcgc ccgggttcat cacggcgcag ggccggagca gcgcgtccga 1080
cgcgagcggg ttcgtgttca cctcgtgcac cgtcggcggc gccgccccgg cgtacctggg 1140
ccgcgcgtgg cgtgcctacg cgcgcgtcgt gttctaccgg acagccatgt ccgccgccgt 1200
cgtcggcctc ggctgggacg cctgggacta caagggcaaa gagtgagtct tgagtcatgc 1260
actactagaa aaagtatttt cgcgggaaaa ataggtcttc gaggtggacg ccggctccgc 1320
ctgcaaagat cgttttcgca ggcggcacct tatgttgtat gcctgcgata agctgtcttc 1380
gcaggcaaac ggccaactcc tcctcggtaa tcattttcgt cggcgggatt ttcgctccga 1440
tggccatatc cgcctgcgaa aaaaaaaagc ctaacaacgg tgaaaatgtt ttttctaagt 1500
agtgatggtc atcgccgtcg tcggtgcatg atcggaagtg gctgattgct acgtggatgc 1560
gtgcagggag acgctggaga tggtggagtc ggggtgcacg gggccggggt cgaacaggac 1620
ggggagggtg ccatgggaga agacgctgag cggggaggag ctcgccaagc tcgtcgacat 1680
ctcgtacgtc tcccgcgacg gctggctcgc cgctcagcca cggtagctag cgctcaacat 1740
ggggattgca aataaagtg 1759
<210> 20
<211> 1008
<212> DNA
<213> Oryza sativa
<400> 20
atgaagcaag ttctggcccc cataatagcc ttggtcattg gaattggaac actagctttc 60
atggccatta gtcctcaggt ttgccatgct gctgcaggag gaagtgctac tgtggcgaga 120
agcatcttcg tcagcaagaa gggtagtggt gctgatttca caaggataca agacgccatc 180
aactccgtcc cattcgccaa ccggcggtgg atccggattc acatcgccgc cggcgtctac 240
aaggagaagg tgagcatacc ggcgaacaag agcttcatct tgctggaagg cgaggggagg 300
cagcagacgt cgatcgagtg ggcggaccac gccggcggcg gcggcgggga ctccggcacg 360
gccgactcgc cgacgttcgc ctcgtacgcc gccgacttca tggcccgcga catcaccttc 420
aagaacacgt acgggaggat ggcgccggcg gtggcggcgc tggtggccgg agaccggtcg 480
gcgttctacc ggtgcggctt cgtggggctg caggacacgc tgagcgacct gctggggagg 540
cactactacg agcgctgcta cgtcgagggc gccgtcgact tcatcttcgg ggaagcccag 600
tccatcttcc accgctgcca catctccacc gcggcggcgg cggcgcccgg gttcatcacg 660
gcgcagggcc ggagcagcgc gtccgacgcg agcgggttcg tgttcacctc gtgcaccgtc 720
ggcggcgccg ccccggcgta cctgggccgc gcgtggcgtg cctacgcgcg cgtcgtgttc 780
taccggacag ccatgtccgc cgccgtcgtc ggcctcggct gggacgcctg ggactacaag 840
ggcaaagagg agacgctgga gatggtggag tcggggtgca cggggccggg gtcgaacagg 900
acggggaggg tgccatggga gaagacgctg agcggggagg agctcgccaa gctcgtcgac 960
atctcgtacg tctcccgcga cggctggctc gccgctcagc cacggtag 1008
<210> 21
<211> 335
<212> PRT
<213> Oryza sativa
<400> 21
Met Lys Gln Val Leu Ala Pro Ile Ile Ala Leu Val Ile Gly Ile Gly
1 5 10 15
Thr Leu Ala Phe Met Ala Ile Ser Pro Gln Val Cys His Ala Ala Ala
20 25 30
Gly Gly Ser Ala Thr Val Ala Arg Ser Ile Phe Val Ser Lys Lys Gly
35 40 45
Ser Gly Ala Asp Phe Thr Arg Ile Gln Asp Ala Ile Asn Ser Val Pro
50 55 60
Phe Ala Asn Arg Arg Trp Ile Arg Ile His Ile Ala Ala Gly Val Tyr
65 70 75 80
Lys Glu Lys Val Ser Ile Pro Ala Asn Lys Ser Phe Ile Leu Leu Glu
85 90 95
Gly Glu Gly Arg Gln Gln Thr Ser Ile Glu Trp Ala Asp His Ala Gly
100 105 110
Gly Gly Gly Gly Asp Ser Gly Thr Ala Asp Ser Pro Thr Phe Ala Ser
115 120 125
Tyr Ala Ala Asp Phe Met Ala Arg Asp Ile Thr Phe Lys Asn Thr Tyr
130 135 140
Gly Arg Met Ala Pro Ala Val Ala Ala Leu Val Ala Gly Asp Arg Ser
145 150 155 160
Ala Phe Tyr Arg Cys Gly Phe Val Gly Leu Gln Asp Thr Leu Ser Asp
165 170 175
Leu Leu Gly Arg His Tyr Tyr Glu Arg Cys Tyr Val Glu Gly Ala Val
180 185 190
Asp Phe Ile Phe Gly Glu Ala Gln Ser Ile Phe His Arg Cys His Ile
195 200 205
Ser Thr Ala Ala Ala Ala Ala Pro Gly Phe Ile Thr Ala Gln Gly Arg
210 215 220
Ser Ser Ala Ser Asp Ala Ser Gly Phe Val Phe Thr Ser Cys Thr Val
225 230 235 240
Gly Gly Ala Ala Pro Ala Tyr Leu Gly Arg Ala Trp Arg Ala Tyr Ala
245 250 255
Arg Val Val Phe Tyr Arg Thr Ala Met Ser Ala Ala Val Val Gly Leu
260 265 270
Gly Trp Asp Ala Trp Asp Tyr Lys Gly Lys Glu Glu Thr Leu Glu Met
275 280 285
Val Glu Ser Gly Cys Thr Gly Pro Gly Ser Asn Arg Thr Gly Arg Val
290 295 300
Pro Trp Glu Lys Thr Leu Ser Gly Glu Glu Leu Ala Lys Leu Val Asp
305 310 315 320
Ile Ser Tyr Val Ser Arg Asp Gly Trp Leu Ala Ala Gln Pro Arg
325 330 335
<210> 22
<211> 688
<212> DNA
<213> Oryza sativa
<400> 22
cttctcactc ttctcgcgtc cttgcaggct ttttcctcca tcgtcgtcag cccatcgatg 60
gcagttcgtc ccacatcgat tgccataggg gcagcatcaa tagatagcat catctcgatg 120
gtctgcgagc tcgagctcga gatgtggtat ttccccatct catgcgaatc ctttgagcct 180
tccggatgcc aactcctgaa gcgtccaggg cgtatccgtc tccatcgtca ggcgctgctg 240
ttgttcaaac aagctgatct accgtctctc ctttcaaggt gaacacctat gcccagtctt 300
cccttccaac cctgtttctt ctacttcacc ttcactccat cattccccat ttgcatgaat 360
accattttgt ttagttacat agttcaagtc gtcattcatt acttcgacaa acagtctgcc 420
gagttcatca actcagctgc tggggccatc accgtctcct acgtcatgca gctactgact 480
ggtaccatct ttatccatca ttaaataaaa acctggatcg atctatgaat atcccctgat 540
cctaattttg catcttaata ttgaatcaac aatttatttt ctaaacttgg ctgcagggag 600
gatatgcata atgtgctttt tacagaggga caatgttcta ggtgtttgca aagagcgcgt 660
gtgagggaac aaatccatac taaggcag 688
<210> 23
<211> 396
<212> DNA
<213> Oryza sativa
<400> 23
atggcagttc gtcccacatc gattgccata ggggcagcat caatagatag catcatctcg 60
atggtctgcg agctcgagct cgagatgtgg tatttcccca tctcatgcga atcctttgag 120
ccttccggat gccaactcct gaagcgtcca gggcgtatcc gtctccatcg tcaggcgctg 180
ctgttgttca aacaagctga tctaccgtct ctcctttcaa gttacatagt tcaagtcgtc 240
attcattact tcgacaaaca gtctgccgag ttcatcaact cagctgctgg ggccatcacc 300
gtctcctacg tcatgcagct actgactggg aggatatgca taatgtgctt tttacagagg 360
gacaatgttc taggtgtttg caaagagcgc gtgtga 396
<210> 24
<211> 131
<212> PRT
<213> Oryza sativa
<400> 24
Met Ala Val Arg Pro Thr Ser Ile Ala Ile Gly Ala Ala Ser Ile Asp
1 5 10 15
Ser Ile Ile Ser Met Val Cys Glu Leu Glu Leu Glu Met Trp Tyr Phe
20 25 30
Pro Ile Ser Cys Glu Ser Phe Glu Pro Ser Gly Cys Gln Leu Leu Lys
35 40 45
Arg Pro Gly Arg Ile Arg Leu His Arg Gln Ala Leu Leu Leu Phe Lys
50 55 60
Gln Ala Asp Leu Pro Ser Leu Leu Ser Ser Tyr Ile Val Gln Val Val
65 70 75 80
Ile His Tyr Phe Asp Lys Gln Ser Ala Glu Phe Ile Asn Ser Ala Ala
85 90 95
Gly Ala Ile Thr Val Ser Tyr Val Met Gln Leu Leu Thr Gly Arg Ile
100 105 110
Cys Ile Met Cys Phe Leu Gln Arg Asp Asn Val Leu Gly Val Cys Lys
115 120 125
Glu Arg Val
130
<210> 25
<211> 300
<212> DNA
<213> Oryza sativa
<400> 25
cagcgatgga tattgaaccg ggaggcggtg gcggcgtcga ggaggaggcg gcggcggtcg 60
ggaggcgacg gcttggagga ggaggaggcg gcgtcagcgg tggcggagga ggaggaggcg 120
acgacggcgg tggatctgag agagtgagag tgtgagagag tgagaggcgg cgtcggcggt 180
ggataaggac ccgtggatga ggattgacta agtaaaccct agtcaatcct catctctatc 240
gggcagacta ttatacccga tgcagatgac actcatccgt gacgggcgtt agtactctgc 300
<210> 26
<211> 165
<212> DNA
<213> Oryza sativa
<400> 26
atggatattg aaccgggagg cggtggcggc gtcgaggagg aggcggcggc ggtcgggagg 60
cgacggcttg gaggaggagg aggcggcgtc agcggtggcg gaggaggagg aggcgacgac 120
ggcggtggat ctgagagaat gacactcatc cgtgacgggc gttag 165
<210> 27
<211> 54
<212> PRT
<213> Oryza sativa
<400> 27
Met Asp Ile Glu Pro Gly Gly Gly Gly Gly Val Glu Glu Glu Ala Ala
1 5 10 15
Ala Val Gly Arg Arg Arg Leu Gly Gly Gly Gly Gly Gly Val Ser Gly
20 25 30
Gly Gly Gly Gly Gly Gly Asp Asp Gly Gly Gly Ser Glu Arg Met Thr
35 40 45
Leu Ile Arg Asp Gly Arg
50
<210> 28
<211> 4245
<212> DNA
<213> Oryza sativa
<400> 28
catgtggagg ttgaaggttg cggagagcgg tggcacgccg ttgctgcggt cgacgaatgg 60
cttccttggg cgagcggtgt gggagttcga ccccgaccac ggcacgccgg aggaccgcgc 120
cgacgtcgag agggtgcgcc gtgagttcac cgaccaccgc cttcaccacc gggagtcagc 180
tgacctcctc atgcgcatgc aagtatatat taattacata cgctcattca tcccttagtt 240
ttttaaatat ataatgtcgc tattcgttta ttaataaaag tattcagttg ttatatactt 300
ctattttggt tatggttata tcatcactgc agtacattaa gaacgactta tatatagaga 360
gagaaagcat tatgaacggt caaatatatg tttaaaaatc aataatctca tatattaaag 420
aacaaaggaa gtaataaaca cgaatggctc tgtatctttc gaaaatcaat aaccacctca 480
tatattaaag aaaaaaggga gtaataaaca cgaatggttc tatatctctt atttgtatat 540
attatataat aattacttat tagttaaact gcaatttact ccaatacttt ttgagaaacg 600
caatttaccc caatatgaaa ctgtacggtg tgttcgcttt catttgattt tgttgcaggg 660
tttcgttcat ttcatacggt gttattttca tttccttttg tgttgcaatt tgcatagcaa 720
aacaagcatc aacgtcgtcg ttatcgtatt ccacccgtca ataataagct tggagagaag 780
gaggaggtga ctgaagaaat cgccatggct tcgttgagac gagctctcga cgagttctct 840
tctctgcaag cagacgacgg acactggcct ggagacttca gtggtgttat gttcatcatg 900
cctggcttgg tagtatattt aaattgtatc tgtctctggc taaataattt atgcttagct 960
atttcgtggt ttaatttctg gtgatctagc taatttctgt acaaataaat gcagatattt 1020
gccttatatg tcactggatc actggacact atcatatcac cagagcatcg gcgggagata 1080
tgtcgctata tctataacca tcaggcatat ttttttccgt atatatggac gactgaccat 1140
aaaataatta taatatgctc atgtattcat ggtttaataa ttttaattat agaagaacgt 1200
gtgttactcc agtacttgca gtacgaatgt ttataaaaac aaaaatgggt ggtttgcata 1260
aattaaagca cgagttcaga cttatttgct cagacaaaat ataagcatat ttttctaagt 1320
aacaattaaa tgtcattagt ataaaaaaca ctgagtaaat ttcccaaaac tacatataac 1380
ttaaccaaac tgtgtcaaaa ctatagattt aagataacgt atcacaaaac acaaatttac 1440
catcaaaatt atcattaaaa tattgcaaaa gtacatatct aataatgttt ttgtcacaag 1500
actataggtt ttatttaaat tactcgaact atatattatt atattagagt aataagttat 1560
gatgaatttg gtgctaaacc tataattttg tgataacttt attattaaat ctataatatt 1620
atgatactcg ctcttaaatc tgcaattccg cgataaattt aatattaatt ctatagtttt 1680
atgatacacc attttaaata tataatttta taatagcttg gttagagaat atgtaatttt 1740
gtgaaattca ctttaaaaac atttgatttt ccatattttg tgactgcaaa ttctgcacat 1800
gcatgtgtta tatagaacga ggatggtgga tggggcagtt tgatgttgag ctcgagcacc 1860
atgtttggca catgttcaaa ctatatcacc cttaggcttc tcggtgaaga gacaagcaat 1920
gagcaattag ccaaagggcg catatggatc atattgcatg gcggcactac ctttgttcca 1980
caatggggaa agatatggct ctcggtctgt ctactatatt atcgcatgaa tccacacaat 2040
aatgaatttt gctaaacaat tgctttcgtt gatttttgta gggttcagtt tactttcgaa 2100
acatcttgat ttatataaac atgactaata taatggttaa ctaaatgata ggtatattta 2160
gcactttcta atcgtaccaa ccgattcata ttttaattaa agtactttca cacgattggt 2220
tttatagtac aaggttttca ctttcgcgcc aaaaatttcg gccattttgg tagaaacgaa 2280
atttctggat ttttgggatt ttcggcgttt tttagaccat tttttgaaat tttaacacaa 2340
tttaactgaa tttgactaaa ttcacaaaaa agttagaaaa atcgaaaatt tcaaccgaga 2400
taatcatttg cctgggtaag aattggaggc agggttcaat ctatcgggaa ggggaaaaat 2460
ttcaggggtc accgaaattt cgaaaatctt tcagaaattt cgaacggaat tttcaaacaa 2520
aatttgaatt taaacaataa aaataaagaa attttaccaa gatcagagta tagatgagca 2580
tcgagtctat tcaatccaaa gcagaggaac aggagattga aacacagtaa cacgtaagga 2640
tgcgaggctt gtgcggcttg tgtcgtcgtc cggtcatcgt cgtgctgccg aagagcgatg 2700
gagccgccag cggcaactct agccaaacgg aggatgcgag gcccgcggct tgtatcgtcg 2760
tcgtggtgcc gaagagccac ggagacgccg ccggccactc caaccaaccc gccgcctccg 2820
gttccggcca ccgccgcggc cgctgttcgc tagcttggga ggatggcgac ctgctgcctg 2880
ctgggcgagc tgtcggagga tagcgaagca gcagcagcct ttggtatata agtgaggagg 2940
ggataggaaa ttgaggattt ttaccatgaa cattgttttg ccgaaatttt tgaccggagc 3000
tcatgtttat caggacccac caatagaccc gaaatttcgt aaatttcgtt ccgaaatttt 3060
gaaccctgat tagagggaag gtagaaaaaa aaagcccaaa aggcaaaaag aaaggagaaa 3120
aaacgaaata agaaaagaaa aacggcggaa aaaaacctaa gcccgaaaaa aaaagggacg 3180
gaggaaaaga aaaaagatgg aaaaaagaaa ataacagcga aaaaagaaaa aaggacaaaa 3240
agagaaccgg aaaccttaat tattatatat ataggtataa aaaagaaaaa acagcgaaaa 3300
aagaaaaaaa cggacggaaa gagaacagga aaccttactt atttttctta ttaggtatag 3360
ataagccata tgactttttt ctagtcaaac ttctttaagt ttgaccaatt ttatataaaa 3420
atttagtagt attttcaaca caaaacaaac atattatcag aatatattca atgttatatt 3480
taatgaaact aatttgatat tttagatgtt gttaaatttt tctataaatt tgggccaact 3540
tgacaaagtt taattatgaa aaaaatcaaa cgacttataa tataacgcgt tgattgattt 3600
cggacaaatt aatttgattc actacagata cttggagtgt acgagtgggc aggaaacaac 3660
cccatcttcc ctgagctatg gctgacccca caatttcttc cttttcatcc aggtatatat 3720
atactagtaa taatcatact gtatactcct acatcaaata gtagctagtt gcacacaaat 3780
caaaaacagt ttaatggcaa tgcctggagt ttatatcaga cttaaggttc atgaaaattc 3840
tttaattttg aactgaattt acttttgtgc aggcaaattc tggtgcttaa ctcgcatggt 3900
atacctgccg atggcctacc tatacggcaa gaagttcgtg ggccccaccg cgccgaccat 3960
cctggcgctg cgagaggaga tttactcggc ccactacctc acaatcgatt gggcccaggc 4020
ccgcagcgcc tgcgccaagg taatttttgg gccgggccga atttgttggg cttcttgttg 4080
ggggaggagt gccccgcgag gagaattttt tttatttata atttttttta ttaaaagttt 4140
tacaaaaata attttccatt ttgaaaattg acgaatgtag tcgcctaccg ccctctggga 4200
gggcgatttt taaaaatcgc cctctcagag ggcggcgaaa atgac 4245
<210> 29
<211> 867
<212> DNA
<213> Oryza sativa
<400> 29
atgtggaggt tgaaggttgc ggagagcggt ggcacgccgt tgctgcggtc gacgaatggc 60
ttccttgggc gagcggtgtg ggagttcgac cccgaccacg gcacgccgga ggaccgcgcc 120
gacgtcgaga gggtgcgccg tgagttcacc gaccaccgcc ttcaccaccg ggagtcagct 180
gacctcctca tgcgcatgca acaaaacaag catcaacgtc gtcgttatcg tattccaccc 240
gtcaataata agcttggaga gaaggaggag gtgactgaag aaatcgccat ggcttcgttg 300
agacgagctc tcgacgagtt ctcttctctg caagcagacg acggacactg gcctggagac 360
ttcagtggtg ttatgttcat catgcctggc ttgaacgagg atggtggatg gggcagtttg 420
atgttgagct cgagcaccat gtttggcaca tgttcaaact atatcaccct taggcttctc 480
ggtgaagaga caagcaatga gcaattagcc aaagggcgca tatggatcat attgcatggc 540
ggcactacct ttgttccaca atggggaaag atatggctct cgatacttgg agtgtacgag 600
tgggcaggaa acaaccccat cttccctgag ctatggctga ccccacaatt tcttcctttt 660
catccaggca aattctggtg cttaactcgc atggtatacc tgccgatggc ctacctatac 720
ggcaagaagt tcgtgggccc caccgcgccg accatcctgg cgctgcgaga ggagatttac 780
tcggcccact acctcacaat cgattgggcc caggcccgca gcgcctgcgc caagtcgcct 840
accgccctct gggagggcga tttttaa 867
<210> 30
<211> 288
<212> PRT
<213> Oryza sativa
<400> 30
Met Trp Arg Leu Lys Val Ala Glu Ser Gly Gly Thr Pro Leu Leu Arg
1 5 10 15
Ser Thr Asn Gly Phe Leu Gly Arg Ala Val Trp Glu Phe Asp Pro Asp
20 25 30
His Gly Thr Pro Glu Asp Arg Ala Asp Val Glu Arg Val Arg Arg Glu
35 40 45
Phe Thr Asp His Arg Leu His His Arg Glu Ser Ala Asp Leu Leu Met
50 55 60
Arg Met Gln Gln Asn Lys His Gln Arg Arg Arg Tyr Arg Ile Pro Pro
65 70 75 80
Val Asn Asn Lys Leu Gly Glu Lys Glu Glu Val Thr Glu Glu Ile Ala
85 90 95
Met Ala Ser Leu Arg Arg Ala Leu Asp Glu Phe Ser Ser Leu Gln Ala
100 105 110
Asp Asp Gly His Trp Pro Gly Asp Phe Ser Gly Val Met Phe Ile Met
115 120 125
Pro Gly Leu Asn Glu Asp Gly Gly Trp Gly Ser Leu Met Leu Ser Ser
130 135 140
Ser Thr Met Phe Gly Thr Cys Ser Asn Tyr Ile Thr Leu Arg Leu Leu
145 150 155 160
Gly Glu Glu Thr Ser Asn Glu Gln Leu Ala Lys Gly Arg Ile Trp Ile
165 170 175
Ile Leu His Gly Gly Thr Thr Phe Val Pro Gln Trp Gly Lys Ile Trp
180 185 190
Leu Ser Ile Leu Gly Val Tyr Glu Trp Ala Gly Asn Asn Pro Ile Phe
195 200 205
Pro Glu Leu Trp Leu Thr Pro Gln Phe Leu Pro Phe His Pro Gly Lys
210 215 220
Phe Trp Cys Leu Thr Arg Met Val Tyr Leu Pro Met Ala Tyr Leu Tyr
225 230 235 240
Gly Lys Lys Phe Val Gly Pro Thr Ala Pro Thr Ile Leu Ala Leu Arg
245 250 255
Glu Glu Ile Tyr Ser Ala His Tyr Leu Thr Ile Asp Trp Ala Gln Ala
260 265 270
Arg Ser Ala Cys Ala Lys Ser Pro Thr Ala Leu Trp Glu Gly Asp Phe
275 280 285
<210> 31
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning cDNA of OsCCP1 gene
<400> 31
cacttcctag caattaagat catggtg 27
<210> 32
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning cDNA of OsCCP1 gene
<400> 32
ctggagatgg aggatggata ttgtc 25
<210> 33
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning cDNA of OsGPR89A gene
<400> 33
ctgctgaggt ttccccttgc gagatccatc t 31
<210> 34
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning cDNA of OsGPR89A gene
<400> 34
ccgctgaggt gtggacacaa cattacaacc ggc 33
<210> 35
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning cDNA of OsDN-DTP13 gene
<400> 35
cacctcgtgt gctacaagtg cta 23
<210> 36
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning cDNA of OsDN-DTP13 gene
<400> 36
gggagtagct aaaagtggtt cagtg 25
<210> 37
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning cDNA of OsCTPS1 gene
<400> 37
ctgctgaggc gatgaagtac gtgctggtga c 31
<210> 38
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning cDNA of OsCTPS1 gene
<400> 38
ccgctgaggg ctatgctgct aagtatgaag aatgc 35
<210> 39
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning gDNA of OsDN-DTP14 gene
<400> 39
ctgctgaggc ggaggcagtg acaacgacag c 31
<210> 40
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning gDNA of OsDN-DTP14 gene
<400> 40
ccgctgaggg atcctcaaac gacgtctcca ggg 33
<210> 41
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning gDNA of OsLPA1 gene
<400> 41
ctgctgaggc cttcctcctc tctctctcac tcttg 35
<210> 42
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning gDNA of OsLPA1 gene
<400> 42
ccgctgagga gctcctccca agtccaaatc cac 33
<210> 43
<211> 36
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning gDNA of OsPE1 gene
<400> 43
ctgctgaggg atctatcaag tttgtcaggc gagatg 36
<210> 44
<211> 34
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning gDNA of OsPE1 gene
<400> 44
ccgctgaggc actttatttg caatccccat gttg 34
<210> 45
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning gDNA of OsDN-DTP15 gene
<400> 45
ctgctgaggc ttctcactct tctcgcgtcc ttg 33
<210> 46
<211> 37
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning gDNA of OsDN-DTP15 gene
<400> 46
ccgctgaggc tgccttagta tggatttgtt ccctcac 37
<210> 47
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning gDNA of OsDN-DTP16 gene
<400> 47
ctgctgaggc agcgatggat attgaaccgg gag 33
<210> 48
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning gDNA of OsDN-DTP16 gene
<400> 48
ccgctgaggg cagagtacta acgcccgtca cg 32
<210> 49
<211> 34
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning gDNA of OsCAS2 gene
<400> 49
ctgctgaggc atgtggaggt tgaaggttgc ggag 34
<210> 50
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning gDNA of OsCAS2 gene
<400> 50
ccgctgaggg tcattttcgc cgccctctga gag 33
<210> 51
<211> 21
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for real-time PCR analysis of OsLPA1 gene
<400> 51
tggcacaaac tcgatagcta c 21
<210> 52
<211> 18
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for real-time PCR analysis of OsLPA1 gene
<400> 52
ccgcccgttg atttcgag 18
<210> 53
<211> 1080
<212> DNA
<213> Oryza sativa
<400> 53
atggcgtccg tggatctgac cccgaggcag gcgaggaagg cgtacggcgg cgatggtggg 60
acctactacg agtggagccc cgcagacctg cccatgctgg aactcgccaa catcggcggc 120
gccaagctgt cgctcaacgc cggtggcctc gccctgccca gcttttccga ctccggaaag 180
gttgcgtatg ttcttcaagg caagggcact tgtggcattg tcctgccgga ggcgagcaag 240
gagaaggtga tcgccgtgaa ggagggagac tccctggcac tcccctttgg cgtggtgaca 300
tggtggcata acctcccgga gtccccaatc gagctcgtca tcctcttcct cggcgacaca 360
tcgaaggcgc acaaggccgg ccaattcaca aacatgcagc tcaccggtgc caccggcatc 420
ttcaccggct tctccacgga gttcgtcggc cgcgcatggg acctcgccga gtccgacgcc 480
gtcaagctcg tgtccagcca gcctgcctcc ggcatcgtca agatcaagtc cggccagaag 540
ctccccgagc cgtcggccgc cgaccgcgag ggcatggcgc tcaactgcct ggaggcgccg 600
ctcgacgtgg acatcaagaa cggcggccgc gtggtggtgc tcaacacggc gaacctgccg 660
atggtgaagg aggtcgggct cggcgccgac ctggtgagga tcgacggcca ctccatgtgc 720
tcgccggggt tctcgtgcga ctcggcgtac caggtcacct acttcatccg cggcagcggc 780
cgcgtccagg tggtcggcgc cgacgggaag cgcgtgctgg acacccacgt cgagggcggc 840
aacctgttca tcgtgccgcg cttctgcgtc gtctccaaga tcgccgacgc ctccggcctg 900
cagtggttct ccattatcac cacaccaaac ccgatcttca gtcacctggc ggggaagacg 960
tcggtgtgga aggcgatctc gccggaggtg ctggaggcgt cgttcaacgc gacgccggag 1020
atggagaagc tgttccggtc caaaaggatc gactcggaga tcttcttcgc gcccaactga 1080
<210> 54
<211> 359
<212> PRT
<213> Oryza sativa
<400> 54
Met Ala Ser Val Asp Leu Thr Pro Arg Gln Ala Arg Lys Ala Tyr Gly
1 5 10 15
Gly Asp Gly Gly Thr Tyr Tyr Glu Trp Ser Pro Ala Asp Leu Pro Met
20 25 30
Leu Glu Leu Ala Asn Ile Gly Gly Ala Lys Leu Ser Leu Asn Ala Gly
35 40 45
Gly Leu Ala Leu Pro Ser Phe Ser Asp Ser Gly Lys Val Ala Tyr Val
50 55 60
Leu Gln Gly Lys Gly Thr Cys Gly Ile Val Leu Pro Glu Ala Ser Lys
65 70 75 80
Glu Lys Val Ile Ala Val Lys Glu Gly Asp Ser Leu Ala Leu Pro Phe
85 90 95
Gly Val Val Thr Trp Trp His Asn Leu Pro Glu Ser Pro Ile Glu Leu
100 105 110
Val Ile Leu Phe Leu Gly Asp Thr Ser Lys Ala His Lys Ala Gly Gln
115 120 125
Phe Thr Asn Met Gln Leu Thr Gly Ala Thr Gly Ile Phe Thr Gly Phe
130 135 140
Ser Thr Glu Phe Val Gly Arg Ala Trp Asp Leu Ala Glu Ser Asp Ala
145 150 155 160
Val Lys Leu Val Ser Ser Gln Pro Ala Ser Gly Ile Val Lys Ile Lys
165 170 175
Ser Gly Gln Lys Leu Pro Glu Pro Ser Ala Ala Asp Arg Glu Gly Met
180 185 190
Ala Leu Asn Cys Leu Glu Ala Pro Leu Asp Val Asp Ile Lys Asn Gly
195 200 205
Gly Arg Val Val Val Leu Asn Thr Ala Asn Leu Pro Met Val Lys Glu
210 215 220
Val Gly Leu Gly Ala Asp Leu Val Arg Ile Asp Gly His Ser Met Cys
225 230 235 240
Ser Pro Gly Phe Ser Cys Asp Ser Ala Tyr Gln Val Thr Tyr Phe Ile
245 250 255
Arg Gly Ser Gly Arg Val Gln Val Val Gly Ala Asp Gly Lys Arg Val
260 265 270
Leu Asp Thr His Val Glu Gly Gly Asn Leu Phe Ile Val Pro Arg Phe
275 280 285
Cys Val Val Ser Lys Ile Ala Asp Ala Ser Gly Leu Gln Trp Phe Ser
290 295 300
Ile Ile Thr Thr Pro Asn Pro Ile Phe Ser His Leu Ala Gly Lys Thr
305 310 315 320
Ser Val Trp Lys Ala Ile Ser Pro Glu Val Leu Glu Ala Ser Phe Asn
325 330 335
Ala Thr Pro Glu Met Glu Lys Leu Phe Arg Ser Lys Arg Ile Asp Ser
340 345 350
Glu Ile Phe Phe Ala Pro Asn
355
<210> 55
<211> 1119
<212> DNA
<213> Zea mays
<400> 55
atggtgcacc ggacttccat agccgatgtg catgtgatgt gcatggatct aagcccaaag 60
aaacccaaca aggccagcgc cagcgacggc ggagcctact acgactggtc ccccgccgat 120
ctgcccatgc tcggcgttgc ctccattggt gccgccaagc tctgcctcac cgccggaggt 180
cttgccctac ccagctactc cgactctgcc aagatcgcct acgtcctcca aggcaaaggt 240
atattcggcg tggttctccc ggaggcgacc aaggagaagg tcatctccgt caaggaaggc 300
gacgcgctgg cgctcccctt cggcgtcgtc acctggtggc acaacaacgc cgacgccgct 360
atctccgacc tcgtggtgct cttcctcggc gacacctcca cgggccacaa gccgggccag 420
ttcacaaact tccagctcac cggctccacg ggcatcttca cgggcttctc cacagagttc 480
gttgcccgcg catgggacct cacccaggac gacgccgcca agctcgtctc cacccagccc 540
ggatccggca ttgtcagggt caaggacggg cacaagatgc ccgaggcgcg cgacgaggac 600
aggcagggcc tggtcctcaa ctgcctagag gcgccgcttg acgtcgacat caagaacgga 660
gggcgcgtcg tggtcctcaa cacccagaac ctgccgctcg tcaaggaggt cgggctcggt 720
gctgaccttg tcaggatcga cgcccactcc atgtgctcgc ccggcttctc ctgcgactcc 780
gcctaccagg tcacctacat cgtgcgcggc agtggccgcg tccaggttgt tgggatcgac 840
ggcacgcgag tgctcgagac ccgcgccgag ggaggctgcc tcttcatcgt gccaaggttc 900
ttcgttgtct caaagatcgc cgacgaaacc ggcatggagt ggttctccat catcaccacc 960
cccaacccaa tctttagcca cttggcgggc cggacatcgg tgtggaaggc aatatctccg 1020
gcggtcttgc agtcgtcgtt caacaccacg ccggagatgg agaagctgtt ccgtagcaag 1080
aggcttgact cggagatctt ctttgcgcct tcaaactga 1119
<210> 56
<211> 372
<212> PRT
<213> Zea mays
<400> 56
Met Val His Arg Thr Ser Ile Ala Asp Val His Val Met Cys Met Asp
1 5 10 15
Leu Ser Pro Lys Lys Pro Asn Lys Ala Ser Ala Ser Asp Gly Gly Ala
20 25 30
Tyr Tyr Asp Trp Ser Pro Ala Asp Leu Pro Met Leu Gly Val Ala Ser
35 40 45
Ile Gly Ala Ala Lys Leu Cys Leu Thr Ala Gly Gly Leu Ala Leu Pro
50 55 60
Ser Tyr Ser Asp Ser Ala Lys Ile Ala Tyr Val Leu Gln Gly Lys Gly
65 70 75 80
Ile Phe Gly Val Val Leu Pro Glu Ala Thr Lys Glu Lys Val Ile Ser
85 90 95
Val Lys Glu Gly Asp Ala Leu Ala Leu Pro Phe Gly Val Val Thr Trp
100 105 110
Trp His Asn Asn Ala Asp Ala Ala Ile Ser Asp Leu Val Val Leu Phe
115 120 125
Leu Gly Asp Thr Ser Thr Gly His Lys Pro Gly Gln Phe Thr Asn Phe
130 135 140
Gln Leu Thr Gly Ser Thr Gly Ile Phe Thr Gly Phe Ser Thr Glu Phe
145 150 155 160
Val Ala Arg Ala Trp Asp Leu Thr Gln Asp Asp Ala Ala Lys Leu Val
165 170 175
Ser Thr Gln Pro Gly Ser Gly Ile Val Arg Val Lys Asp Gly His Lys
180 185 190
Met Pro Glu Ala Arg Asp Glu Asp Arg Gln Gly Leu Val Leu Asn Cys
195 200 205
Leu Glu Ala Pro Leu Asp Val Asp Ile Lys Asn Gly Gly Arg Val Val
210 215 220
Val Leu Asn Thr Gln Asn Leu Pro Leu Val Lys Glu Val Gly Leu Gly
225 230 235 240
Ala Asp Leu Val Arg Ile Asp Ala His Ser Met Cys Ser Pro Gly Phe
245 250 255
Ser Cys Asp Ser Ala Tyr Gln Val Thr Tyr Ile Val Arg Gly Ser Gly
260 265 270
Arg Val Gln Val Val Gly Ile Asp Gly Thr Arg Val Leu Glu Thr Arg
275 280 285
Ala Glu Gly Gly Cys Leu Phe Ile Val Pro Arg Phe Phe Val Val Ser
290 295 300
Lys Ile Ala Asp Glu Thr Gly Met Glu Trp Phe Ser Ile Ile Thr Thr
305 310 315 320
Pro Asn Pro Ile Phe Ser His Leu Ala Gly Arg Thr Ser Val Trp Lys
325 330 335
Ala Ile Ser Pro Ala Val Leu Gln Ser Ser Phe Asn Thr Thr Pro Glu
340 345 350
Met Glu Lys Leu Phe Arg Ser Lys Arg Leu Asp Ser Glu Ile Phe Phe
355 360 365
Ala Pro Ser Asn
370
<210> 57
<211> 1128
<212> DNA
<213> Sorghum bicolor
<400> 57
atggtgcagc ggacttccac cgcccaagtg atgtccatgg acctcagccc aaagaaaccc 60
aacaaggcct acggcagcga tggcggcgcc tactacgact ggtcccccgc cgacctgccc 120
atgctcggcg ctgcctccat tggcgccgcc aagctctgcc tctccgccgg aggccttgct 180
ctgcctagct actctgactc tgccaaggtc gcctacgtcc tccaaggtaa gggtacgtgc 240
ggcgtggttc tgccggaggc gaccaaggag aaggtgatcc ccgtgaagga gggtgactcg 300
ctcgcgctcc ctttcggcgt cgtcacctgg tggcacaacg cgcacgccgc ctgcagcagc 360
tccgactccg acgacctcgt ggtcctcttc ctcggcgaca cgtccacggg ccacaagcgg 420
ggccagttca ccaacttcca gctcaccggc tccacgggca tcttcacggg cctgtccacc 480
gagttcgtcg cccgcgcatg ggacctcacc ccggacgccg ccgccgagct cgtctcctcc 540
cagcccggcg ccggcatcat cagggtcaag gacgggcacc ggatgcccca ggcccgcgac 600
gaggacaggg agggcatggt cctcaactgc ctggaggcgc cgctcgacgt cgacatcaag 660
aacgggggcc gcgtcgtcgt cctcaacacc cggaacctgc cgctcgtgga ggaggtcggt 720
ctcggcgccg acctcgtcag gatcgacgcg cactccatgt gctcgccggg gttctcctgc 780
gactccgcct accaggtcac ctacatcgtg cgcggcagtg gccgcgtcca ggtcgttggg 840
atcgacggca cgcgggtgct ggagacccgc gccgagggag gctgcctctt catcgtgccc 900
aggttcttcg ttgtctccaa gatcgccgac gaaactggca tggagtggtt ctccatcatc 960
accaccccca acccaatctt tagccacttg gcgggcagga catcggtgtg gaaggcaata 1020
tctcctgcgg tcctggaggc gtcgttcaac accacgccgg agaaggagaa gctgttccgt 1080
tccaagaggc ttgactcgga gatcttcttc gcgcctgctt caaactaa 1128
<210> 58
<211> 375
<212> PRT
<213> Sorghum bicolor
<400> 58
Met Val Gln Arg Thr Ser Thr Ala Gln Val Met Ser Met Asp Leu Ser
1 5 10 15
Pro Lys Lys Pro Asn Lys Ala Tyr Gly Ser Asp Gly Gly Ala Tyr Tyr
20 25 30
Asp Trp Ser Pro Ala Asp Leu Pro Met Leu Gly Ala Ala Ser Ile Gly
35 40 45
Ala Ala Lys Leu Cys Leu Ser Ala Gly Gly Leu Ala Leu Pro Ser Tyr
50 55 60
Ser Asp Ser Ala Lys Val Ala Tyr Val Leu Gln Gly Lys Gly Thr Cys
65 70 75 80
Gly Val Val Leu Pro Glu Ala Thr Lys Glu Lys Val Ile Pro Val Lys
85 90 95
Glu Gly Asp Ser Leu Ala Leu Pro Phe Gly Val Val Thr Trp Trp His
100 105 110
Asn Ala His Ala Ala Cys Ser Ser Ser Asp Ser Asp Asp Leu Val Val
115 120 125
Leu Phe Leu Gly Asp Thr Ser Thr Gly His Lys Arg Gly Gln Phe Thr
130 135 140
Asn Phe Gln Leu Thr Gly Ser Thr Gly Ile Phe Thr Gly Leu Ser Thr
145 150 155 160
Glu Phe Val Ala Arg Ala Trp Asp Leu Thr Pro Asp Ala Ala Ala Glu
165 170 175
Leu Val Ser Ser Gln Pro Gly Ala Gly Ile Ile Arg Val Lys Asp Gly
180 185 190
His Arg Met Pro Gln Ala Arg Asp Glu Asp Arg Glu Gly Met Val Leu
195 200 205
Asn Cys Leu Glu Ala Pro Leu Asp Val Asp Ile Lys Asn Gly Gly Arg
210 215 220
Val Val Val Leu Asn Thr Arg Asn Leu Pro Leu Val Glu Glu Val Gly
225 230 235 240
Leu Gly Ala Asp Leu Val Arg Ile Asp Ala His Ser Met Cys Ser Pro
245 250 255
Gly Phe Ser Cys Asp Ser Ala Tyr Gln Val Thr Tyr Ile Val Arg Gly
260 265 270
Ser Gly Arg Val Gln Val Val Gly Ile Asp Gly Thr Arg Val Leu Glu
275 280 285
Thr Arg Ala Glu Gly Gly Cys Leu Phe Ile Val Pro Arg Phe Phe Val
290 295 300
Val Ser Lys Ile Ala Asp Glu Thr Gly Met Glu Trp Phe Ser Ile Ile
305 310 315 320
Thr Thr Pro Asn Pro Ile Phe Ser His Leu Ala Gly Arg Thr Ser Val
325 330 335
Trp Lys Ala Ile Ser Pro Ala Val Leu Glu Ala Ser Phe Asn Thr Thr
340 345 350
Pro Glu Lys Glu Lys Leu Phe Arg Ser Lys Arg Leu Asp Ser Glu Ile
355 360 365
Phe Phe Ala Pro Ala Ser Asn
370 375
<210> 59
<211> 1071
<212> DNA
<213> Arabidopsis thaliana
<400> 59
atggaattgg atctatcacc aaggctcccg aaaaaggtgt acggaggaga cggtggttcg 60
tactttgcat ggtgccctga agagttgcca atgctccgag atggtaacat tggagcctct 120
aagctcgctc ttgagaagta tggcttggct cttcctcgct actctgactc ccccaaggtc 180
gcttatgttc ttcaaggagc tggaacagct ggaatcgtgc tccctgagaa ggaggagaaa 240
gtgatagcaa tcaagaaagg agactccata gctttacctt ttggtgtagt gacatggtgg 300
ttcaacaatg aggacactga gctagtcgtt ctcttccttg gtgagactca caagggtcac 360
aaagctggac agttcaccga cttttaccta accggatcca atggaatctt caccggtttc 420
tctactgagt ttgttggcag agcttgggat ctcgatgaga ccaccgtgaa gaagcttgtc 480
ggatctcaga caggtaacgg tatcgtgaag gttgatgcga gcttgaagat gcctgaacca 540
aagaaaggtg accgaaaagg gtttgttttg aactgtttgg aggctcctct tgatgttgac 600
attaaggatg gaggaagagt tgttgtgttg aacacaaaga atcttccttt ggttggtgaa 660
gttggatttg gagctgatct tgtgagaatc gatggacact ctatgtgttc tcctggattc 720
tcttgtgact cggctcttca agttacttac attgttggtg gaagtggtcg tgttcagatt 780
gttggtgctg atgggaagag agttcttgag actcatgtga aagctggtgt tttgttcatt 840
gttcctaggt tctttgttgt ctccaagatt gctgattctg atggcttgtc ttggttctcc 900
attgtgacca ctcctgatcc aatattcact catttggccg ggaggacttc ggtgtggaag 960
gctttgtcgc cggaggtttt gcaggcagcg ttcaaggtgg atcccgaggt tgagaaggct 1020
ttccgatcta agaggacctc tgatgccatt ttcttctctc cttccaacta g 1071
<210> 60
<211> 356
<212> PRT
<213> Arabidopsis thaliana
<400> 60
Met Glu Leu Asp Leu Ser Pro Arg Leu Pro Lys Lys Val Tyr Gly Gly
1 5 10 15
Asp Gly Gly Ser Tyr Phe Ala Trp Cys Pro Glu Glu Leu Pro Met Leu
20 25 30
Arg Asp Gly Asn Ile Gly Ala Ser Lys Leu Ala Leu Glu Lys Tyr Gly
35 40 45
Leu Ala Leu Pro Arg Tyr Ser Asp Ser Pro Lys Val Ala Tyr Val Leu
50 55 60
Gln Gly Ala Gly Thr Ala Gly Ile Val Leu Pro Glu Lys Glu Glu Lys
65 70 75 80
Val Ile Ala Ile Lys Lys Gly Asp Ser Ile Ala Leu Pro Phe Gly Val
85 90 95
Val Thr Trp Trp Phe Asn Asn Glu Asp Thr Glu Leu Val Val Leu Phe
100 105 110
Leu Gly Glu Thr His Lys Gly His Lys Ala Gly Gln Phe Thr Asp Phe
115 120 125
Tyr Leu Thr Gly Ser Asn Gly Ile Phe Thr Gly Phe Ser Thr Glu Phe
130 135 140
Val Gly Arg Ala Trp Asp Leu Asp Glu Thr Thr Val Lys Lys Leu Val
145 150 155 160
Gly Ser Gln Thr Gly Asn Gly Ile Val Lys Val Asp Ala Ser Leu Lys
165 170 175
Met Pro Glu Pro Lys Lys Gly Asp Arg Lys Gly Phe Val Leu Asn Cys
180 185 190
Leu Glu Ala Pro Leu Asp Val Asp Ile Lys Asp Gly Gly Arg Val Val
195 200 205
Val Leu Asn Thr Lys Asn Leu Pro Leu Val Gly Glu Val Gly Phe Gly
210 215 220
Ala Asp Leu Val Arg Ile Asp Gly His Ser Met Cys Ser Pro Gly Phe
225 230 235 240
Ser Cys Asp Ser Ala Leu Gln Val Thr Tyr Ile Val Gly Gly Ser Gly
245 250 255
Arg Val Gln Ile Val Gly Ala Asp Gly Lys Arg Val Leu Glu Thr His
260 265 270
Val Lys Ala Gly Val Leu Phe Ile Val Pro Arg Phe Phe Val Val Ser
275 280 285
Lys Ile Ala Asp Ser Asp Gly Leu Ser Trp Phe Ser Ile Val Thr Thr
290 295 300
Pro Asp Pro Ile Phe Thr His Leu Ala Gly Arg Thr Ser Val Trp Lys
305 310 315 320
Ala Leu Ser Pro Glu Val Leu Gln Ala Ala Phe Lys Val Asp Pro Glu
325 330 335
Val Glu Lys Ala Phe Arg Ser Lys Arg Thr Ser Asp Ala Ile Phe Phe
340 345 350
Ser Pro Ser Asn
355
<210> 61
<211> 1071
<212> DNA
<213> Glycine max
<400> 61
atggacattg atctttctcc tcagttgccc aagaaagttt acggtgccaa tggtggctcc 60
tactacgcat ggtccccctc cgaccttccc atgctccacc aaggcaacat tggtgccgct 120
aagcttgctc tcaacaaaaa cgcctttgct cttcctcgtt actctgactc ttccaaagtc 180
gcttatgttc ttcaaggtag tggagtggct ggaatagtgt tgcctgaatc agaagagaag 240
gttgttgcaa taaagaaggg tgatgccttg gcactcccct ttggtgttgt gacctggtgg 300
tataacaagg aagaaactga gttggttgtt ctgttccttg gggacacttc caaggctcac 360
aaggctggtg aattcactga cttttttctg actggttcca atggaatctt cactggcttt 420
tccaccgagt ttgtgggcag ggcttgggac ttggaagaga gtcatgtcaa gacccttgtt 480
ggaaaacaac cagccaaagg gattgtgcag ctggaaggaa acatcagcct tcccgaccct 540
aaaccggagc accgaaatgg catggccttg aactgtgaag aggctccatt ggatgttgac 600
atcaagggtg gtggaagggt tgtggtgttg aacaccaaga atcttccctt ggttggcgag 660
gttggactag gggctgatct tgtgaggctg gatggaagag ccatgtgttc gcctggattc 720
tcttgtgatt ctgctttgca ggttacttat attgtcaggg gtagtggccg ggttcaggtt 780
gttggtgttg acggccgtag ggttttggag acaaccgtga aagctggtaa tttgttcatt 840
gtgccaaggt tttttgtggt gtccaagatt gctgatcctg atggactgga gtggttctct 900
atcatcacta cccctaatcc tatatttacc cacttggcag gaagttcttc cgtgtggaag 960
gctttatcac cttcggtttt gcaagctgct ttcaatgtag atccagaagt tgagcaactg 1020
ttccgttcaa agaggactgc cgatgccatt ttcttccctc caccaaacta g 1071
<210> 62
<211> 356
<212> PRT
<213> Glycine max
<400> 62
Met Asp Ile Asp Leu Ser Pro Gln Leu Pro Lys Lys Val Tyr Gly Ala
1 5 10 15
Asn Gly Gly Ser Tyr Tyr Ala Trp Ser Pro Ser Asp Leu Pro Met Leu
20 25 30
His Gln Gly Asn Ile Gly Ala Ala Lys Leu Ala Leu Asn Lys Asn Ala
35 40 45
Phe Ala Leu Pro Arg Tyr Ser Asp Ser Ser Lys Val Ala Tyr Val Leu
50 55 60
Gln Gly Ser Gly Val Ala Gly Ile Val Leu Pro Glu Ser Glu Glu Lys
65 70 75 80
Val Val Ala Ile Lys Lys Gly Asp Ala Leu Ala Leu Pro Phe Gly Val
85 90 95
Val Thr Trp Trp Tyr Asn Lys Glu Glu Thr Glu Leu Val Val Leu Phe
100 105 110
Leu Gly Asp Thr Ser Lys Ala His Lys Ala Gly Glu Phe Thr Asp Phe
115 120 125
Phe Leu Thr Gly Ser Asn Gly Ile Phe Thr Gly Phe Ser Thr Glu Phe
130 135 140
Val Gly Arg Ala Trp Asp Leu Glu Glu Ser His Val Lys Thr Leu Val
145 150 155 160
Gly Lys Gln Pro Ala Lys Gly Ile Val Gln Leu Glu Gly Asn Ile Ser
165 170 175
Leu Pro Asp Pro Lys Pro Glu His Arg Asn Gly Met Ala Leu Asn Cys
180 185 190
Glu Glu Ala Pro Leu Asp Val Asp Ile Lys Gly Gly Gly Arg Val Val
195 200 205
Val Leu Asn Thr Lys Asn Leu Pro Leu Val Gly Glu Val Gly Leu Gly
210 215 220
Ala Asp Leu Val Arg Leu Asp Gly Arg Ala Met Cys Ser Pro Gly Phe
225 230 235 240
Ser Cys Asp Ser Ala Leu Gln Val Thr Tyr Ile Val Arg Gly Ser Gly
245 250 255
Arg Val Gln Val Val Gly Val Asp Gly Arg Arg Val Leu Glu Thr Thr
260 265 270
Val Lys Ala Gly Asn Leu Phe Ile Val Pro Arg Phe Phe Val Val Ser
275 280 285
Lys Ile Ala Asp Pro Asp Gly Leu Glu Trp Phe Ser Ile Ile Thr Thr
290 295 300
Pro Asn Pro Ile Phe Thr His Leu Ala Gly Ser Ser Ser Val Trp Lys
305 310 315 320
Ala Leu Ser Pro Ser Val Leu Gln Ala Ala Phe Asn Val Asp Pro Glu
325 330 335
Val Glu Gln Leu Phe Arg Ser Lys Arg Thr Ala Asp Ala Ile Phe Phe
340 345 350
Pro Pro Pro Asn
355
<210> 63
<211> 1407
<212> DNA
<213> Zea mays
<400> 63
atggggtggg gcacagtggt ttacgagggc gcggtcgtcg gctcgtcgct ggtggggctc 60
ggctgggcgg ggctgtggtt cctgaaccgg cggctgtaca aggagtacga ggagcggcgg 120
gtgctggtgc agatcctctt cggcctcgtc tttgccttct cctgcaacct cttcgagctc 180
gttctcttcg agatcctgcc cgtcctctcc aagcacgcgc gcttcctcaa ctggcacctc 240
gacctcttct gcctcatcct cctcctcgtc ttcgtactcc cctactacca ctgctatctt 300
ctgctccgta actcagggat gaggagggac cgggcgtggc tcgtcgcggc gctctttctg 360
ctggtcttcc tatacgggtt ctggcgcatg gggattcact tccccatgcc ttcaccggag 420
aagggtttct ttacgatgcc gcagttggtc agtaggattg gggtgattgg agttagtgtc 480
atggctgttc tttctggttt tggtgccgtc aatctgccat acagttatct gtcgctattc 540
atcagggaaa ttgatgaaac agacatcaaa accttggaac gccagttgat gcaatccatc 600
gagacatgta ctgctaagaa gaagaaaatt attttgtccc agatggagat ggagaggatt 660
caaggatcag aggagaagtt aaaggccaga tcgtttctga agcgtatagt gggaacagtt 720
gttagatctg tgcaggaaga tcaaactgag caggatataa aaaacttaga agcagaagtc 780
caggcactgg aagagctttc caaacagctg tttcttgaga tatatgaact tcgtcaagct 840
aagatagctg ctgcgtattc tcgaacttgg agaggtcatc ttcagaatct acttggatat 900
gctttatcag tctattgtgt ttataagatg ctcaagtcct tgcagagtgt agtctttaag 960
gagtcaggct ctgttgatcc tgtaacaatg tcaataacga ttttcctgag acattttgac 1020
attggcattg atgttgcact gttatctcag tacatatctt tgatgttcat cgggatgttg 1080
gttgtcatat ctgttcgagg tttcctggca aatgttatga agttcttctt cgctgtttct 1140
agagttggga gtgggtcgac aaccaatgtt gtccttttcc tatcagagat catgggaatg 1200
tacttcatat cttccattct tcttataaga aaaagcctgg caaatgaata tagggtgatc 1260
attactgatg tcttgggtgg tgatatccaa tttgacttct accatcgctg gtttgatgct 1320
atatttgtgg ctagtgcgtt cctctccttg cttcttatat ctgcccaata caccaccagg 1380
caaacagaca agcatccgat tgattga 1407
<210> 64
<211> 468
<212> PRT
<213> Zea mays
<400> 64
Met Gly Trp Gly Thr Val Val Tyr Glu Gly Ala Val Val Gly Ser Ser
1 5 10 15
Leu Val Gly Leu Gly Trp Ala Gly Leu Trp Phe Leu Asn Arg Arg Leu
20 25 30
Tyr Lys Glu Tyr Glu Glu Arg Arg Val Leu Val Gln Ile Leu Phe Gly
35 40 45
Leu Val Phe Ala Phe Ser Cys Asn Leu Phe Glu Leu Val Leu Phe Glu
50 55 60
Ile Leu Pro Val Leu Ser Lys His Ala Arg Phe Leu Asn Trp His Leu
65 70 75 80
Asp Leu Phe Cys Leu Ile Leu Leu Leu Val Phe Val Leu Pro Tyr Tyr
85 90 95
His Cys Tyr Leu Leu Leu Arg Asn Ser Gly Met Arg Arg Asp Arg Ala
100 105 110
Trp Leu Val Ala Ala Leu Phe Leu Leu Val Phe Leu Tyr Gly Phe Trp
115 120 125
Arg Met Gly Ile His Phe Pro Met Pro Ser Pro Glu Lys Gly Phe Phe
130 135 140
Thr Met Pro Gln Leu Val Ser Arg Ile Gly Val Ile Gly Val Ser Val
145 150 155 160
Met Ala Val Leu Ser Gly Phe Gly Ala Val Asn Leu Pro Tyr Ser Tyr
165 170 175
Leu Ser Leu Phe Ile Arg Glu Ile Asp Glu Thr Asp Ile Lys Thr Leu
180 185 190
Glu Arg Gln Leu Met Gln Ser Ile Glu Thr Cys Thr Ala Lys Lys Lys
195 200 205
Lys Ile Ile Leu Ser Gln Met Glu Met Glu Arg Ile Gln Gly Ser Glu
210 215 220
Glu Lys Leu Lys Ala Arg Ser Phe Leu Lys Arg Ile Val Gly Thr Val
225 230 235 240
Val Arg Ser Val Gln Glu Asp Gln Thr Glu Gln Asp Ile Lys Asn Leu
245 250 255
Glu Ala Glu Val Gln Ala Leu Glu Glu Leu Ser Lys Gln Leu Phe Leu
260 265 270
Glu Ile Tyr Glu Leu Arg Gln Ala Lys Ile Ala Ala Ala Tyr Ser Arg
275 280 285
Thr Trp Arg Gly His Leu Gln Asn Leu Leu Gly Tyr Ala Leu Ser Val
290 295 300
Tyr Cys Val Tyr Lys Met Leu Lys Ser Leu Gln Ser Val Val Phe Lys
305 310 315 320
Glu Ser Gly Ser Val Asp Pro Val Thr Met Ser Ile Thr Ile Phe Leu
325 330 335
Arg His Phe Asp Ile Gly Ile Asp Val Ala Leu Leu Ser Gln Tyr Ile
340 345 350
Ser Leu Met Phe Ile Gly Met Leu Val Val Ile Ser Val Arg Gly Phe
355 360 365
Leu Ala Asn Val Met Lys Phe Phe Phe Ala Val Ser Arg Val Gly Ser
370 375 380
Gly Ser Thr Thr Asn Val Val Leu Phe Leu Ser Glu Ile Met Gly Met
385 390 395 400
Tyr Phe Ile Ser Ser Ile Leu Leu Ile Arg Lys Ser Leu Ala Asn Glu
405 410 415
Tyr Arg Val Ile Ile Thr Asp Val Leu Gly Gly Asp Ile Gln Phe Asp
420 425 430
Phe Tyr His Arg Trp Phe Asp Ala Ile Phe Val Ala Ser Ala Phe Leu
435 440 445
Ser Leu Leu Leu Ile Ser Ala Gln Tyr Thr Thr Arg Gln Thr Asp Lys
450 455 460
His Pro Ile Asp
465
<210> 65
<211> 1182
<212> DNA
<213> Sorghum bicolor
<400> 65
atggggtggg gcacagtggt ttacgagggg gcggtcgtcg gctcgtcgct ggtggggctg 60
ggctgggcgg ggctgtggtt cctgaaccgg cggctgtaca aggagtacga ggagcggcgg 120
gtgctggtgc agatcctctt cggcctcgtc ttcgccttct cctgcaacct cttcgagctc 180
gttctcttcg agatcctccc cgtcctctcc aagcacgcgc gcttcctcaa ctggcacctc 240
gacctcttct gcctcattct cctcctcgtc ttcgtactcc cctactacca ctgctatctt 300
ctgctccgta actcaggtcc cggcccccaa tcaatctccc attgcttcat ttctctctcc 360
ctttctgggg gggtgaggag ggagcgggcg tggctcgtcg cggcgctctt tctgctggtc 420
ttcctatacg ggttctggcg catggggatt cacttcccca tgccttcacc ggagaagggt 480
ttgttgacgg aaattgatga agcagacatc aaaaccttgg aaaggcagct gatgcaatcc 540
atggagacat gtacttctaa gaagaagaaa attatcttgt cccagatgga gatggagagg 600
attcaaggat cagaggagat agctgctgcg tattctcgaa cgtggagagg gcatcttcag 660
aatctacttg gatatgcttt gtcggtgtat tgtgtttata agatgctcaa gtccttgcag 720
agtgtagtct ttaaggagtc aggctctgtt gatcctgtaa caatgacaat aacaattttc 780
ctgagacatt ttgacattgg cattgatgtt gcactgttat ctcagtacat atctttgatg 840
ttcatcggga tgttggttgt catatctgtt cgaggtttct tggcaaatgt tatgaagttc 900
ttctttgccg tttctagagt tgggagtgga tcgacaacca atgttgtcct tttcctatca 960
gagatcatgg gcatgtactt catatcttcc attcttctta taagaaaaag cctggcaaat 1020
gaatataggg tgatcattac tgatgttttg ggtggtgata tccaatttga cttctaccac 1080
cgctggtttg atgctatatt tgtggctagt gcgttcctgt ccttgcttct gatttctgcc 1140
caatacacca ccaggcaaac agacaagcat ccgattgatt ga 1182
<210> 66
<211> 393
<212> PRT
<213> Sorghum bicolor
<400> 66
Met Gly Trp Gly Thr Val Val Tyr Glu Gly Ala Val Val Gly Ser Ser
1 5 10 15
Leu Val Gly Leu Gly Trp Ala Gly Leu Trp Phe Leu Asn Arg Arg Leu
20 25 30
Tyr Lys Glu Tyr Glu Glu Arg Arg Val Leu Val Gln Ile Leu Phe Gly
35 40 45
Leu Val Phe Ala Phe Ser Cys Asn Leu Phe Glu Leu Val Leu Phe Glu
50 55 60
Ile Leu Pro Val Leu Ser Lys His Ala Arg Phe Leu Asn Trp His Leu
65 70 75 80
Asp Leu Phe Cys Leu Ile Leu Leu Leu Val Phe Val Leu Pro Tyr Tyr
85 90 95
His Cys Tyr Leu Leu Leu Arg Asn Ser Gly Pro Gly Pro Gln Ser Ile
100 105 110
Ser His Cys Phe Ile Ser Leu Ser Leu Ser Gly Gly Val Arg Arg Glu
115 120 125
Arg Ala Trp Leu Val Ala Ala Leu Phe Leu Leu Val Phe Leu Tyr Gly
130 135 140
Phe Trp Arg Met Gly Ile His Phe Pro Met Pro Ser Pro Glu Lys Gly
145 150 155 160
Leu Leu Thr Glu Ile Asp Glu Ala Asp Ile Lys Thr Leu Glu Arg Gln
165 170 175
Leu Met Gln Ser Met Glu Thr Cys Thr Ser Lys Lys Lys Lys Ile Ile
180 185 190
Leu Ser Gln Met Glu Met Glu Arg Ile Gln Gly Ser Glu Glu Ile Ala
195 200 205
Ala Ala Tyr Ser Arg Thr Trp Arg Gly His Leu Gln Asn Leu Leu Gly
210 215 220
Tyr Ala Leu Ser Val Tyr Cys Val Tyr Lys Met Leu Lys Ser Leu Gln
225 230 235 240
Ser Val Val Phe Lys Glu Ser Gly Ser Val Asp Pro Val Thr Met Thr
245 250 255
Ile Thr Ile Phe Leu Arg His Phe Asp Ile Gly Ile Asp Val Ala Leu
260 265 270
Leu Ser Gln Tyr Ile Ser Leu Met Phe Ile Gly Met Leu Val Val Ile
275 280 285
Ser Val Arg Gly Phe Leu Ala Asn Val Met Lys Phe Phe Phe Ala Val
290 295 300
Ser Arg Val Gly Ser Gly Ser Thr Thr Asn Val Val Leu Phe Leu Ser
305 310 315 320
Glu Ile Met Gly Met Tyr Phe Ile Ser Ser Ile Leu Leu Ile Arg Lys
325 330 335
Ser Leu Ala Asn Glu Tyr Arg Val Ile Ile Thr Asp Val Leu Gly Gly
340 345 350
Asp Ile Gln Phe Asp Phe Tyr His Arg Trp Phe Asp Ala Ile Phe Val
355 360 365
Ala Ser Ala Phe Leu Ser Leu Leu Leu Ile Ser Ala Gln Tyr Thr Thr
370 375 380
Arg Gln Thr Asp Lys His Pro Ile Asp
385 390
<210> 67
<211> 1407
<212> DNA
<213> Arabidopsis thaliana
<400> 67
atgagttacg gatgggcgat atacgaaggc acggtggtga tagcttcact aagtctctta 60
ggatgggcag gtttatggtt tcttaatcgg agattataca aagagtatga agagaaacga 120
gctttggttc aaatcatttt cagtgtcgtc ttcgctttct cttgtaatct attgcagctc 180
gttttgttcg agatcatacc tgttctctct agagaggcaa ggatgataaa ctggaaggtg 240
gatctttttt gtttgatact tcttctggtt ttcatgttgc cgtattacca ttgctatttg 300
atgcttcgta atagtggtgt aagaagggag cgtgcctctg ttggggcttt cttattcttg 360
tcagcattcc tttacgcatt ctggcgtatg ggagttcatt tccctatgcc ttcagcagat 420
aaagggtttt ttaccatgcc tcagctggtc agtagaattg gggtcattgg tgtgacctta 480
atggctgtct tatcaggatt tggagctgta aatttaccct acagctatat atccctcttc 540
attagggaga ttgaagaagc agatataata tctttggaaa ggcaactgat tcagtcaact 600
gagacgtgca tagcaaagaa gaagaaaatt attttgtgtc aattggaggt ggaacgaaat 660
caaggatcag aagagaatca gaaacgtagc tctttcttca gaagaattgt tgggactgtt 720
gtgcggtcgg ttcaagatga ccaaaaggaa caagacataa aaatattgga agcggaggtg 780
gaagccttag aggagctgtc aaaacagtta tttttggaag tatatgagct gcgtcaagca 840
aaggatgctg ctgcttattc tagaacttgg aagggtcatg tgcagaactt acttggttat 900
gcatgttcca tttattgtgt gtataaaatg ttgaagtctc ttcaaagtgt tgtcttcaaa 960
gaggccggta caaaagatcc tgtcacgacg atgatcagca tattcttgcg gttgtttgat 1020
ataggagtag atgctgcact cctctcacag tatatatccc tgctgttcat tgggatgttg 1080
attgtgattt ctgtgagggg attcctgacg aacctgatga agtttttctt tgctgtctca 1140
agagtgggaa gtgggtcttc aagcaatgtt gtacttttcc tatctgaaat catgggaatg 1200
tactttttgt cttcaattct tcttatcaga aaaagcttga gaaatgagta caggggaatc 1260
ataacagatg tgttgggtgg agatattcag tttgatttct atcatcgatg gtttgatgca 1320
atttttgtgg cgagtgcttt tctttctctc gttttgcttt ctgcacatta cacgtctcgt 1380
caatctgata agcacgccat cgagtaa 1407
<210> 68
<211> 468
<212> PRT
<213> Arabidopsis thaliana
<400> 68
Met Ser Tyr Gly Trp Ala Ile Tyr Glu Gly Thr Val Val Ile Ala Ser
1 5 10 15
Leu Ser Leu Leu Gly Trp Ala Gly Leu Trp Phe Leu Asn Arg Arg Leu
20 25 30
Tyr Lys Glu Tyr Glu Glu Lys Arg Ala Leu Val Gln Ile Ile Phe Ser
35 40 45
Val Val Phe Ala Phe Ser Cys Asn Leu Leu Gln Leu Val Leu Phe Glu
50 55 60
Ile Ile Pro Val Leu Ser Arg Glu Ala Arg Met Ile Asn Trp Lys Val
65 70 75 80
Asp Leu Phe Cys Leu Ile Leu Leu Leu Val Phe Met Leu Pro Tyr Tyr
85 90 95
His Cys Tyr Leu Met Leu Arg Asn Ser Gly Val Arg Arg Glu Arg Ala
100 105 110
Ser Val Gly Ala Phe Leu Phe Leu Ser Ala Phe Leu Tyr Ala Phe Trp
115 120 125
Arg Met Gly Val His Phe Pro Met Pro Ser Ala Asp Lys Gly Phe Phe
130 135 140
Thr Met Pro Gln Leu Val Ser Arg Ile Gly Val Ile Gly Val Thr Leu
145 150 155 160
Met Ala Val Leu Ser Gly Phe Gly Ala Val Asn Leu Pro Tyr Ser Tyr
165 170 175
Ile Ser Leu Phe Ile Arg Glu Ile Glu Glu Ala Asp Ile Ile Ser Leu
180 185 190
Glu Arg Gln Leu Ile Gln Ser Thr Glu Thr Cys Ile Ala Lys Lys Lys
195 200 205
Lys Ile Ile Leu Cys Gln Leu Glu Val Glu Arg Asn Gln Gly Ser Glu
210 215 220
Glu Asn Gln Lys Arg Ser Ser Phe Phe Arg Arg Ile Val Gly Thr Val
225 230 235 240
Val Arg Ser Val Gln Asp Asp Gln Lys Glu Gln Asp Ile Lys Ile Leu
245 250 255
Glu Ala Glu Val Glu Ala Leu Glu Glu Leu Ser Lys Gln Leu Phe Leu
260 265 270
Glu Val Tyr Glu Leu Arg Gln Ala Lys Asp Ala Ala Ala Tyr Ser Arg
275 280 285
Thr Trp Lys Gly His Val Gln Asn Leu Leu Gly Tyr Ala Cys Ser Ile
290 295 300
Tyr Cys Val Tyr Lys Met Leu Lys Ser Leu Gln Ser Val Val Phe Lys
305 310 315 320
Glu Ala Gly Thr Lys Asp Pro Val Thr Thr Met Ile Ser Ile Phe Leu
325 330 335
Arg Leu Phe Asp Ile Gly Val Asp Ala Ala Leu Leu Ser Gln Tyr Ile
340 345 350
Ser Leu Leu Phe Ile Gly Met Leu Ile Val Ile Ser Val Arg Gly Phe
355 360 365
Leu Thr Asn Leu Met Lys Phe Phe Phe Ala Val Ser Arg Val Gly Ser
370 375 380
Gly Ser Ser Ser Asn Val Val Leu Phe Leu Ser Glu Ile Met Gly Met
385 390 395 400
Tyr Phe Leu Ser Ser Ile Leu Leu Ile Arg Lys Ser Leu Arg Asn Glu
405 410 415
Tyr Arg Gly Ile Ile Thr Asp Val Leu Gly Gly Asp Ile Gln Phe Asp
420 425 430
Phe Tyr His Arg Trp Phe Asp Ala Ile Phe Val Ala Ser Ala Phe Leu
435 440 445
Ser Leu Val Leu Leu Ser Ala His Tyr Thr Ser Arg Gln Ser Asp Lys
450 455 460
His Ala Ile Glu
465
<210> 69
<211> 1407
<212> DNA
<213> Glycine max
<400> 69
atgggttggg gaaggggaat gttggaaggt gtggtggtgg tgggatgtct gtgtttactg 60
ggttgggcgg gtctgtggtt tttgaaccgt cgactctaca aagagtacga agagaaacgc 120
gtcctcgtcc aaatcatctt cagcgtcgtc tttgccttct cctgcaatct tctccagctc 180
gtcatcttcg aaatcattcc aatcctctcc aaagaggcaa gagtggtgaa ctggaaggtg 240
gacttgttct gtttgatctt gttgctggtc ttccttctcc cctattacca ttgttacttg 300
atgctcaaaa acaatggcgt cagggctgag agggctgcac ttggtgctat tctctttctg 360
ttcgcttttc tctatgcctt ttggcgcatg ggcattcact ttccaatgcc atctccagat 420
aaaggtttct tcaccatgcc tcaactggtg agtcgaattg gggttattgg tgttactgtc 480
atggctgtgc tatctggttt tggtgctgta aacctcccat acagttactt atcacttttc 540
atcagagaga ttgaggaaac agaaatcaag gccttggaaa gacagctaat gcaatcgatt 600
gagacctgtg tatcaaagaa aaagaaaatt attctttgcc aaatggagat ggataacaag 660
caaggatctg aagagaagtt aaatgctaga tctttaatta aacgaattgt tggtacggtt 720
gttcgatctg tgcaagagga tcaaaaagag caagatatta aagggttgga agcagaagtt 780
ctggccttag aagagctttc aaagcaattg ttcttggagg tctatgaact tcgtcaagca 840
aaggaagctg ctgcttattc tcgaacttgg agaggacaca tgcagaatct acttggctat 900
gcttgttctg tatattgtgt gtataaaatg atcaagtcat tgcaaagtgt tgttttcaaa 960
caggatggtt cagttgatcc cgtgacaagg ataataagta tattccttca gttctttgat 1020
ataggaataa atgcagcctt attatcacag tacatttcct tgctattcat tggaatgttg 1080
gttgtgatat cagtgcgtgg attcttaaca aacctaatga agttcttttt tgctgtttca 1140
agagttggaa gtggatcttc aagcaatgta gttctgtttt tgtctgagat aatgggaatg 1200
tattttgtat cttctattct tttaatcagg aaaagcttag caactgaata caggattatt 1260
ataacagagg tattgggtgg tgatatccag tttgactttt atcaccggtg gtttgatgca 1320
atttttgtgg ctagtgcatt cctttcactg cttttacttt ctgcacatta tacatctcgc 1380
caaattgaca aacatccaat tgattaa 1407
<210> 70
<211> 468
<212> PRT
<213> Glycine max
<400> 70
Met Gly Trp Gly Arg Gly Met Leu Glu Gly Val Val Val Val Gly Cys
1 5 10 15
Leu Cys Leu Leu Gly Trp Ala Gly Leu Trp Phe Leu Asn Arg Arg Leu
20 25 30
Tyr Lys Glu Tyr Glu Glu Lys Arg Val Leu Val Gln Ile Ile Phe Ser
35 40 45
Val Val Phe Ala Phe Ser Cys Asn Leu Leu Gln Leu Val Ile Phe Glu
50 55 60
Ile Ile Pro Ile Leu Ser Lys Glu Ala Arg Val Val Asn Trp Lys Val
65 70 75 80
Asp Leu Phe Cys Leu Ile Leu Leu Leu Val Phe Leu Leu Pro Tyr Tyr
85 90 95
His Cys Tyr Leu Met Leu Lys Asn Asn Gly Val Arg Ala Glu Arg Ala
100 105 110
Ala Leu Gly Ala Ile Leu Phe Leu Phe Ala Phe Leu Tyr Ala Phe Trp
115 120 125
Arg Met Gly Ile His Phe Pro Met Pro Ser Pro Asp Lys Gly Phe Phe
130 135 140
Thr Met Pro Gln Leu Val Ser Arg Ile Gly Val Ile Gly Val Thr Val
145 150 155 160
Met Ala Val Leu Ser Gly Phe Gly Ala Val Asn Leu Pro Tyr Ser Tyr
165 170 175
Leu Ser Leu Phe Ile Arg Glu Ile Glu Glu Thr Glu Ile Lys Ala Leu
180 185 190
Glu Arg Gln Leu Met Gln Ser Ile Glu Thr Cys Val Ser Lys Lys Lys
195 200 205
Lys Ile Ile Leu Cys Gln Met Glu Met Asp Asn Lys Gln Gly Ser Glu
210 215 220
Glu Lys Leu Asn Ala Arg Ser Leu Ile Lys Arg Ile Val Gly Thr Val
225 230 235 240
Val Arg Ser Val Gln Glu Asp Gln Lys Glu Gln Asp Ile Lys Gly Leu
245 250 255
Glu Ala Glu Val Leu Ala Leu Glu Glu Leu Ser Lys Gln Leu Phe Leu
260 265 270
Glu Val Tyr Glu Leu Arg Gln Ala Lys Glu Ala Ala Ala Tyr Ser Arg
275 280 285
Thr Trp Arg Gly His Met Gln Asn Leu Leu Gly Tyr Ala Cys Ser Val
290 295 300
Tyr Cys Val Tyr Lys Met Ile Lys Ser Leu Gln Ser Val Val Phe Lys
305 310 315 320
Gln Asp Gly Ser Val Asp Pro Val Thr Arg Ile Ile Ser Ile Phe Leu
325 330 335
Gln Phe Phe Asp Ile Gly Ile Asn Ala Ala Leu Leu Ser Gln Tyr Ile
340 345 350
Ser Leu Leu Phe Ile Gly Met Leu Val Val Ile Ser Val Arg Gly Phe
355 360 365
Leu Thr Asn Leu Met Lys Phe Phe Phe Ala Val Ser Arg Val Gly Ser
370 375 380
Gly Ser Ser Ser Asn Val Val Leu Phe Leu Ser Glu Ile Met Gly Met
385 390 395 400
Tyr Phe Val Ser Ser Ile Leu Leu Ile Arg Lys Ser Leu Ala Thr Glu
405 410 415
Tyr Arg Ile Ile Ile Thr Glu Val Leu Gly Gly Asp Ile Gln Phe Asp
420 425 430
Phe Tyr His Arg Trp Phe Asp Ala Ile Phe Val Ala Ser Ala Phe Leu
435 440 445
Ser Leu Leu Leu Leu Ser Ala His Tyr Thr Ser Arg Gln Ile Asp Lys
450 455 460
His Pro Ile Asp
465
<210> 71
<211> 1833
<212> DNA
<213> Oryza sativa
<400> 71
atgccggaag caccgaccgc caagacagca ccggcctacg gctacgcccc cggggcgcac 60
gccgaggcgc tcgagttcat cgagcacgtc acggcgaacg ccgggcaggt gcagcggcgc 120
gtgctcggcg agatcctggc gcagaacgcg ccggccgagt acctgcgccg gtacggaatc 180
cccgggtccc ccgacgttgt cgacgccttc cgccgcctcg tcccgctcgt cacatacgag 240
ggcctccagc cagacatcct ccgcatcgcc aacggcgaca cctcgccgat cttctccggg 300
aagcctatct ccgaattcct cacgagctcg ggcacgtcgg gaggggagag gaagctcatg 360
ccgaccatcg ccgacgagat gaacaggcgg tcgctgctgt acagcctgct gatgccggtg 420
atgagccagt cggtgtccgg gctcgacaaa ggcaaggcga tgtacctgct cttcgtgaag 480
gcggagtcgc gcacgccggg cgggctcgcg gcgcggccgg tgctcacaag ctactaccgg 540
agccggcagt tcctcgaccg tccgcgcgac ccctacacat cttacacgag ccccgacgag 600
gccatcctgt gcgtggactc ctaccagagc atgtacgcgc agctgctctg cggcctcgtc 660
caccgcgccg acgtgctgcg cgtgggcgcc gtgttcgcct ccggcttcct ccgcgccatc 720
catttcctcg agaagcactg ggcgcgcctc tgccacgaca tccgcaccgg cgagctcgac 780
ccggagatca ccgaccgcgt ggtgcgcgac gccgtcgggc gggtgctccg cgccgacccg 840
gcgctcgccg acgcgatcga ggacgagtgc gctagggcgt cgtgggaggg catcatccgg 900
cgcctgtggc cacgcaccaa gtacatcgac gtgatcgtga ccggcaccat gtcgcagtac 960
atcccgacgc tcgagttcta cggcggcggc ctgccgctga cgtgcaccat gtacgcctct 1020
tcggagtgct acttcggcct caacctgaat cccatgtgca agcccagcga cgtcgcctac 1080
acgctcatcc ccaccatgtg ctactacgag ttcctccccg tcaattgcaa caatgccact 1140
gccgaggcga gccaccgcga cctcgtcgac ctggtcgacg taaagctcgg gcacgagtac 1200
gagctcgtgg tcaccacgta ttccgggttg tatcgttatc gcgtgggcga cgtgctgagg 1260
gtggcggggt tcaagaacaa ggccccgatg ttcagcttcg tgcggcggca gaacgtggcg 1320
ctgagcgtcg actcggacaa gacggacgag acggagctgc acgcggcggt gagcggcgcg 1380
gtgcagcacc tggcgccgtt cggcgcgtcg ctggtggagt acacgagcta cgcggacgcg 1440
gccaccatcc cgggccacta cgtgctgttc tgggagctgc gcgccggcag cacggcggtg 1500
ccggcgtccg tgttcgagga gtgctgcctg tccgtggagg aggcactgaa cagcgtctac 1560
cggcagggcc gcgcgtgcga caggtccatc ggcccgctcg agatacgcgt cgtggcggag 1620
ggcaccttcg acaagctcat ggactacgcg atcagccggg gcgcgtccat caaccagtac 1680
aaggcgccgc ggtgcgtgcg ccctggcccg gtcgtcgagc tgctcgacgc gagggtgcag 1740
ggcaagtact tcagtcccaa gtgccccaag tggagccccg ggaacaagca atggaacaaa 1800
agcaaggatc tggtcggcaa gggagacgcc taa 1833
<210> 72
<211> 610
<212> PRT
<213> Oryza sativa
<400> 72
Met Pro Glu Ala Pro Thr Ala Lys Thr Ala Pro Ala Tyr Gly Tyr Ala
1 5 10 15
Pro Gly Ala His Ala Glu Ala Leu Glu Phe Ile Glu His Val Thr Ala
20 25 30
Asn Ala Gly Gln Val Gln Arg Arg Val Leu Gly Glu Ile Leu Ala Gln
35 40 45
Asn Ala Pro Ala Glu Tyr Leu Arg Arg Tyr Gly Ile Pro Gly Ser Pro
50 55 60
Asp Val Val Asp Ala Phe Arg Arg Leu Val Pro Leu Val Thr Tyr Glu
65 70 75 80
Gly Leu Gln Pro Asp Ile Leu Arg Ile Ala Asn Gly Asp Thr Ser Pro
85 90 95
Ile Phe Ser Gly Lys Pro Ile Ser Glu Phe Leu Thr Ser Ser Gly Thr
100 105 110
Ser Gly Gly Glu Arg Lys Leu Met Pro Thr Ile Ala Asp Glu Met Asn
115 120 125
Arg Arg Ser Leu Leu Tyr Ser Leu Leu Met Pro Val Met Ser Gln Ser
130 135 140
Val Ser Gly Leu Asp Lys Gly Lys Ala Met Tyr Leu Leu Phe Val Lys
145 150 155 160
Ala Glu Ser Arg Thr Pro Gly Gly Leu Ala Ala Arg Pro Val Leu Thr
165 170 175
Ser Tyr Tyr Arg Ser Arg Gln Phe Leu Asp Arg Pro Arg Asp Pro Tyr
180 185 190
Thr Ser Tyr Thr Ser Pro Asp Glu Ala Ile Leu Cys Val Asp Ser Tyr
195 200 205
Gln Ser Met Tyr Ala Gln Leu Leu Cys Gly Leu Val His Arg Ala Asp
210 215 220
Val Leu Arg Val Gly Ala Val Phe Ala Ser Gly Phe Leu Arg Ala Ile
225 230 235 240
His Phe Leu Glu Lys His Trp Ala Arg Leu Cys His Asp Ile Arg Thr
245 250 255
Gly Glu Leu Asp Pro Glu Ile Thr Asp Arg Val Val Arg Asp Ala Val
260 265 270
Gly Arg Val Leu Arg Ala Asp Pro Ala Leu Ala Asp Ala Ile Glu Asp
275 280 285
Glu Cys Ala Arg Ala Ser Trp Glu Gly Ile Ile Arg Arg Leu Trp Pro
290 295 300
Arg Thr Lys Tyr Ile Asp Val Ile Val Thr Gly Thr Met Ser Gln Tyr
305 310 315 320
Ile Pro Thr Leu Glu Phe Tyr Gly Gly Gly Leu Pro Leu Thr Cys Thr
325 330 335
Met Tyr Ala Ser Ser Glu Cys Tyr Phe Gly Leu Asn Leu Asn Pro Met
340 345 350
Cys Lys Pro Ser Asp Val Ala Tyr Thr Leu Ile Pro Thr Met Cys Tyr
355 360 365
Tyr Glu Phe Leu Pro Val Asn Cys Asn Asn Ala Thr Ala Glu Ala Ser
370 375 380
His Arg Asp Leu Val Asp Leu Val Asp Val Lys Leu Gly His Glu Tyr
385 390 395 400
Glu Leu Val Val Thr Thr Tyr Ser Gly Leu Tyr Arg Tyr Arg Val Gly
405 410 415
Asp Val Leu Arg Val Ala Gly Phe Lys Asn Lys Ala Pro Met Phe Ser
420 425 430
Phe Val Arg Arg Gln Asn Val Ala Leu Ser Val Asp Ser Asp Lys Thr
435 440 445
Asp Glu Thr Glu Leu His Ala Ala Val Ser Gly Ala Val Gln His Leu
450 455 460
Ala Pro Phe Gly Ala Ser Leu Val Glu Tyr Thr Ser Tyr Ala Asp Ala
465 470 475 480
Ala Thr Ile Pro Gly His Tyr Val Leu Phe Trp Glu Leu Arg Ala Gly
485 490 495
Ser Thr Ala Val Pro Ala Ser Val Phe Glu Glu Cys Cys Leu Ser Val
500 505 510
Glu Glu Ala Leu Asn Ser Val Tyr Arg Gln Gly Arg Ala Cys Asp Arg
515 520 525
Ser Ile Gly Pro Leu Glu Ile Arg Val Val Ala Glu Gly Thr Phe Asp
530 535 540
Lys Leu Met Asp Tyr Ala Ile Ser Arg Gly Ala Ser Ile Asn Gln Tyr
545 550 555 560
Lys Ala Pro Arg Cys Val Arg Pro Gly Pro Val Val Glu Leu Leu Asp
565 570 575
Ala Arg Val Gln Gly Lys Tyr Phe Ser Pro Lys Cys Pro Lys Trp Ser
580 585 590
Pro Gly Asn Lys Gln Trp Asn Lys Ser Lys Asp Leu Val Gly Lys Gly
595 600 605
Asp Ala
610
<210> 73
<211> 1809
<212> DNA
<213> Oryza sativa
<400> 73
atgaagtacg tgctggtgac aggaggggtg gtgagcggcc tcggcaaggg cgtgaccgcg 60
agcagcatcg gcgtcgtgct caaggcctgc gggctccgcg tcaccaccat caagattgac 120
ccatacctca acactgatgc tggaaccatg tctccgttcg agcatggtga ggtcttcgtt 180
ttggacgatg gtggagaggt ggacttggac cttggaaatt acgaacgttt tctggacatc 240
aaattgaccc gtgataacaa cataaccacg ggaaaaatat atcagtcagt cattaacaaa 300
gaaaggagag gagactactt ggggaaaacc attcaggttg tgccacatat tacagatgaa 360
attcaagatt ggattgaacg tgtagcgatg aatccggtcg atggtaaaga aggacctcct 420
gatgtttgtg tcatagaact tggtggcacc ataggggata ttgaatcaat gccctttatt 480
gaagcattag gtcaattttc ctaccgtgtt ggacctggaa acttctgtct ggtccatgtc 540
agtcttgttc cagttttaaa tgtagttggt gaacagaaaa ctaaacctac ccagcatagt 600
gttcgtgggc taagaggcct tggattggca cctgacattt tagcatgtcg cagtaccgag 660
ccactggaag aaaacgtgaa agcaaagctc tcacaatttt gtcacgttcc agtctcaagt 720
attattaatc tccatgacgt tacaaacatt tggcacatcc ccttgttgct aagggaccaa 780
agggcccatg aagctattct gaaagttcta gaccttcaat ttgtcggtaa agtaccacga 840
gaacccaagt tggttgaatg gaccgaaaga gccagcaagt ttgacaagtt gaaggctacg 900
gttaagattg cgatggttgg aaaatatact gggctgtccg attcctacct gtctgttcta 960
aaggcacttt tgcatgcatc ggttgctatg ggaagaaagc ttgtagtgga gtgggttcct 1020
tcctgtgatc ttgaagattc tgcagccaaa gagacccctg aagcccataa aaaagcatgg 1080
aagctactca agggtgcaga gggtatactt gtccctggag gctttggaga tagaggtgtt 1140
cagggaaaaa ttcttgctgc aaaatatgca cgagaaaata atgttcctta tctcggcatt 1200
tgcttgggta tgcaaattgc agtgattgat tttgcctgtt ctatcatgaa attaccgggt 1260
gcaaatagca cagagtttga cccagataca atgtcaccct gcgtcatttt catgccagag 1320
ggttccaaaa cccatatggg ggcaactatg cggcttggat caaggagaac atatttccat 1380
gccactgcat gcaaatctgc aaagctgtat ggtaatgcta gattcgtaga cgaaagacat 1440
cgacacagat atgaggtaaa tcctgaaatg gtaccagagt ttgagaaggc cggactttca 1500
tttgttggca aggatgagag tggaagacgc atggagatta ttgaactacc cagtcataaa 1560
tttttcattg gagtacagtt ccatcctgaa ttcaagtcaa gaccagggaa gccatctcca 1620
cttttcttag gattaatagc agcagcatct ggacaactag aaactttgct ccaaccaagt 1680
tccaacattg tcaatccaaa tcctatgccc agatttccca ttcccaaaaa gacgatttac 1740
catgccaaga agccactgga cagcttggta aacggatact ttgcaaatgg caacgtcatc 1800
cacacttga 1809
<210> 74
<211> 602
<212> PRT
<213> Oryza sativa
<400> 74
Met Lys Tyr Val Leu Val Thr Gly Gly Val Val Ser Gly Leu Gly Lys
1 5 10 15
Gly Val Thr Ala Ser Ser Ile Gly Val Val Leu Lys Ala Cys Gly Leu
20 25 30
Arg Val Thr Thr Ile Lys Ile Asp Pro Tyr Leu Asn Thr Asp Ala Gly
35 40 45
Thr Met Ser Pro Phe Glu His Gly Glu Val Phe Val Leu Asp Asp Gly
50 55 60
Gly Glu Val Asp Leu Asp Leu Gly Asn Tyr Glu Arg Phe Leu Asp Ile
65 70 75 80
Lys Leu Thr Arg Asp Asn Asn Ile Thr Thr Gly Lys Ile Tyr Gln Ser
85 90 95
Val Ile Asn Lys Glu Arg Arg Gly Asp Tyr Leu Gly Lys Thr Ile Gln
100 105 110
Val Val Pro His Ile Thr Asp Glu Ile Gln Asp Trp Ile Glu Arg Val
115 120 125
Ala Met Asn Pro Val Asp Gly Lys Glu Gly Pro Pro Asp Val Cys Val
130 135 140
Ile Glu Leu Gly Gly Thr Ile Gly Asp Ile Glu Ser Met Pro Phe Ile
145 150 155 160
Glu Ala Leu Gly Gln Phe Ser Tyr Arg Val Gly Pro Gly Asn Phe Cys
165 170 175
Leu Val His Val Ser Leu Val Pro Val Leu Asn Val Val Gly Glu Gln
180 185 190
Lys Thr Lys Pro Thr Gln His Ser Val Arg Gly Leu Arg Gly Leu Gly
195 200 205
Leu Ala Pro Asp Ile Leu Ala Cys Arg Ser Thr Glu Pro Leu Glu Glu
210 215 220
Asn Val Lys Ala Lys Leu Ser Gln Phe Cys His Val Pro Val Ser Ser
225 230 235 240
Ile Ile Asn Leu His Asp Val Thr Asn Ile Trp His Ile Pro Leu Leu
245 250 255
Leu Arg Asp Gln Arg Ala His Glu Ala Ile Leu Lys Val Leu Asp Leu
260 265 270
Gln Phe Val Gly Lys Val Pro Arg Glu Pro Lys Leu Val Glu Trp Thr
275 280 285
Glu Arg Ala Ser Lys Phe Asp Lys Leu Lys Ala Thr Val Lys Ile Ala
290 295 300
Met Val Gly Lys Tyr Thr Gly Leu Ser Asp Ser Tyr Leu Ser Val Leu
305 310 315 320
Lys Ala Leu Leu His Ala Ser Val Ala Met Gly Arg Lys Leu Val Val
325 330 335
Glu Trp Val Pro Ser Cys Asp Leu Glu Asp Ser Ala Ala Lys Glu Thr
340 345 350
Pro Glu Ala His Lys Lys Ala Trp Lys Leu Leu Lys Gly Ala Glu Gly
355 360 365
Ile Leu Val Pro Gly Gly Phe Gly Asp Arg Gly Val Gln Gly Lys Ile
370 375 380
Leu Ala Ala Lys Tyr Ala Arg Glu Asn Asn Val Pro Tyr Leu Gly Ile
385 390 395 400
Cys Leu Gly Met Gln Ile Ala Val Ile Asp Phe Ala Cys Ser Ile Met
405 410 415
Lys Leu Pro Gly Ala Asn Ser Thr Glu Phe Asp Pro Asp Thr Met Ser
420 425 430
Pro Cys Val Ile Phe Met Pro Glu Gly Ser Lys Thr His Met Gly Ala
435 440 445
Thr Met Arg Leu Gly Ser Arg Arg Thr Tyr Phe His Ala Thr Ala Cys
450 455 460
Lys Ser Ala Lys Leu Tyr Gly Asn Ala Arg Phe Val Asp Glu Arg His
465 470 475 480
Arg His Arg Tyr Glu Val Asn Pro Glu Met Val Pro Glu Phe Glu Lys
485 490 495
Ala Gly Leu Ser Phe Val Gly Lys Asp Glu Ser Gly Arg Arg Met Glu
500 505 510
Ile Ile Glu Leu Pro Ser His Lys Phe Phe Ile Gly Val Gln Phe His
515 520 525
Pro Glu Phe Lys Ser Arg Pro Gly Lys Pro Ser Pro Leu Phe Leu Gly
530 535 540
Leu Ile Ala Ala Ala Ser Gly Gln Leu Glu Thr Leu Leu Gln Pro Ser
545 550 555 560
Ser Asn Ile Val Asn Pro Asn Pro Met Pro Arg Phe Pro Ile Pro Lys
565 570 575
Lys Thr Ile Tyr His Ala Lys Lys Pro Leu Asp Ser Leu Val Asn Gly
580 585 590
Tyr Phe Ala Asn Gly Asn Val Ile His Thr
595 600
<210> 75
<211> 1818
<212> DNA
<213> Zea mays
<400> 75
atgaagtacg tgctggtgac cggcggcgtg gtgagcgggc tcgggaaggg cgtgacggcc 60
agcagcgtcg gggtcgtcct caagtcctgc ggcctccgcg tcacctccat caagatcgat 120
ccatacctca acatcgatgc cggaaccatg tctccgctcg agcatggcga agtgtttgtt 180
ttggacgacg gcggtgaggt ggacttggac cttggaaact acgaacgatt tctggacatc 240
aagctgactc gtgacaacaa cataaccacg gggaagatct accagtctgt tattgacaag 300
gaacgtagag gggattacct aggaaaaact gttcaggttg tgccgcacat cacagatgaa 360
atacaagagt ggattgaacg tgtggcaatg aatccagttg atggcacaga agagccagct 420
gatgtttgtg tcatagaact tggtggcact ataggggaca ttgaatcaat gcctttcatt 480
gaagcattag gtcaattttc ataccgtgta gggcctggaa acttctgctt ggtgcatgtc 540
agtcttgtac cagttttaaa tgtggttggt gagcagaaaa caaagcccac ccaacatagt 600
gttcgcggac ttagaggact tggactcatg cctgatattt tggcatgccg tagtacacag 660
ccacttgaag aacatgtgaa agtgaagctc gcacaatttt gtcatgttcc gatatcaaat 720
atcattaatc tccgtgatgt aacgaacatt tggcacatcc ctttgttgct cagagaccag 780
aaggcccatg aagctatttt gaaagtgtta gacattcagt gtgtgggtaa agttgctcga 840
gaaccccagt tgtctgaatg gactgaaaga gccagcagat gtgacagatt gaaatctccg 900
gttaggattg ctatggttgg aaagtatact ggcctgtcag attcctacct atctgttatc 960
aaggctcttt tgcatgcgtc ggttgctttg gacaggaaac ttgtggtaga ctgggttcct 1020
tcctgtgatc ttgaagattc tgcagcagaa gagaatcttg atgcctacga aaaagcatgg 1080
gaattgttaa agggtgcaga tggtgtgttg gtgccagggg gttttggaga cagaggagtt 1140
caagggaaaa ttctggctgc aaaatatgcg cgagaaaaca acattccata tcttggcatt 1200
tgcttgggca tgcaaattgc agtgattgag tttgcacgtt ctatcatgaa gttgcacggt 1260
gcaaacagca cagagttcga tccaaccaca aaaaccccat gtgttatttt catgccagag 1320
gggtcaaaaa cccatatggg ggcaacaatg cgccttggat caaggaggac ctttttccag 1380
gtcactaact gcaaatctgc taaactgtat gggaatgcta gctacgttga tgaacggcac 1440
cgccacagat acgaggtgaa tcctgatatg gtcccagaat ttgagaaggc agggctttct 1500
ttcgttggca gggatgaaag tggtaaacgc atggagatta tcgaactgcc aactcatagg 1560
tttttcgtcg gtgcacaatt tcatcctgaa ttcaagtcaa ggcccggcaa gccatctccg 1620
cttttcttag gattggtagc agcgtcatca ggtcagcttg atcacctgct ccaacctggt 1680
cttatcagtt cgactggcag acacacctgc agcaaaggag gagcgcaaaa attaaaggct 1740
gggcacgtca tgaagccact gaatggcctg gtgaacgcac actattcagc aaccggcaat 1800
ggcgccattc ccatctag 1818
<210> 76
<211> 605
<212> PRT
<213> Zea mays
<400> 76
Met Lys Tyr Val Leu Val Thr Gly Gly Val Val Ser Gly Leu Gly Lys
1 5 10 15
Gly Val Thr Ala Ser Ser Val Gly Val Val Leu Lys Ser Cys Gly Leu
20 25 30
Arg Val Thr Ser Ile Lys Ile Asp Pro Tyr Leu Asn Ile Asp Ala Gly
35 40 45
Thr Met Ser Pro Leu Glu His Gly Glu Val Phe Val Leu Asp Asp Gly
50 55 60
Gly Glu Val Asp Leu Asp Leu Gly Asn Tyr Glu Arg Phe Leu Asp Ile
65 70 75 80
Lys Leu Thr Arg Asp Asn Asn Ile Thr Thr Gly Lys Ile Tyr Gln Ser
85 90 95
Val Ile Asp Lys Glu Arg Arg Gly Asp Tyr Leu Gly Lys Thr Val Gln
100 105 110
Val Val Pro His Ile Thr Asp Glu Ile Gln Glu Trp Ile Glu Arg Val
115 120 125
Ala Met Asn Pro Val Asp Gly Thr Glu Glu Pro Ala Asp Val Cys Val
130 135 140
Ile Glu Leu Gly Gly Thr Ile Gly Asp Ile Glu Ser Met Pro Phe Ile
145 150 155 160
Glu Ala Leu Gly Gln Phe Ser Tyr Arg Val Gly Pro Gly Asn Phe Cys
165 170 175
Leu Val His Val Ser Leu Val Pro Val Leu Asn Val Val Gly Glu Gln
180 185 190
Lys Thr Lys Pro Thr Gln His Ser Val Arg Gly Leu Arg Gly Leu Gly
195 200 205
Leu Met Pro Asp Ile Leu Ala Cys Arg Ser Thr Gln Pro Leu Glu Glu
210 215 220
His Val Lys Val Lys Leu Ala Gln Phe Cys His Val Pro Ile Ser Asn
225 230 235 240
Ile Ile Asn Leu Arg Asp Val Thr Asn Ile Trp His Ile Pro Leu Leu
245 250 255
Leu Arg Asp Gln Lys Ala His Glu Ala Ile Leu Lys Val Leu Asp Ile
260 265 270
Gln Cys Val Gly Lys Val Ala Arg Glu Pro Gln Leu Ser Glu Trp Thr
275 280 285
Glu Arg Ala Ser Arg Cys Asp Arg Leu Lys Ser Pro Val Arg Ile Ala
290 295 300
Met Val Gly Lys Tyr Thr Gly Leu Ser Asp Ser Tyr Leu Ser Val Ile
305 310 315 320
Lys Ala Leu Leu His Ala Ser Val Ala Leu Asp Arg Lys Leu Val Val
325 330 335
Asp Trp Val Pro Ser Cys Asp Leu Glu Asp Ser Ala Ala Glu Glu Asn
340 345 350
Leu Asp Ala Tyr Glu Lys Ala Trp Glu Leu Leu Lys Gly Ala Asp Gly
355 360 365
Val Leu Val Pro Gly Gly Phe Gly Asp Arg Gly Val Gln Gly Lys Ile
370 375 380
Leu Ala Ala Lys Tyr Ala Arg Glu Asn Asn Ile Pro Tyr Leu Gly Ile
385 390 395 400
Cys Leu Gly Met Gln Ile Ala Val Ile Glu Phe Ala Arg Ser Ile Met
405 410 415
Lys Leu His Gly Ala Asn Ser Thr Glu Phe Asp Pro Thr Thr Lys Thr
420 425 430
Pro Cys Val Ile Phe Met Pro Glu Gly Ser Lys Thr His Met Gly Ala
435 440 445
Thr Met Arg Leu Gly Ser Arg Arg Thr Phe Phe Gln Val Thr Asn Cys
450 455 460
Lys Ser Ala Lys Leu Tyr Gly Asn Ala Ser Tyr Val Asp Glu Arg His
465 470 475 480
Arg His Arg Tyr Glu Val Asn Pro Asp Met Val Pro Glu Phe Glu Lys
485 490 495
Ala Gly Leu Ser Phe Val Gly Arg Asp Glu Ser Gly Lys Arg Met Glu
500 505 510
Ile Ile Glu Leu Pro Thr His Arg Phe Phe Val Gly Ala Gln Phe His
515 520 525
Pro Glu Phe Lys Ser Arg Pro Gly Lys Pro Ser Pro Leu Phe Leu Gly
530 535 540
Leu Val Ala Ala Ser Ser Gly Gln Leu Asp His Leu Leu Gln Pro Gly
545 550 555 560
Leu Ile Ser Ser Thr Gly Arg His Thr Cys Ser Lys Gly Gly Ala Gln
565 570 575
Lys Leu Lys Ala Gly His Val Met Lys Pro Leu Asn Gly Leu Val Asn
580 585 590
Ala His Tyr Ser Ala Thr Gly Asn Gly Ala Ile Pro Ile
595 600 605
<210> 77
<211> 1818
<212> DNA
<213> Sorghum bicolor
<400> 77
atgaagtacg tgctggtgac cggcggagtg gtgagcgggc tcgggaaggg cgtgacggcc 60
agcagcatcg gcgtccttct caagtcctgc ggcttccgcg tcacctccat caagatcgat 120
ccatacctca acaccgatgc cggaaccatg tctcccttcg agcacggcga ggtgtttgtt 180
ttggacgacg gtggtgaggt ggacttggac cttggaaact acgaacgatt tctggacatc 240
aagttgactc gtgacaacaa cataaccacg gggaagatct atcagtctgt cattgacaag 300
gaacgtagag gagattacct aggaaaaact gttcaggttg tgccgcacat cacagatgaa 360
atacaagagt ggattgaacg tgtggcaatg aatccagttg atggcacaga agaggcagct 420
gatgtttgtg tcatagaact tggtggcact ataggggaca ttgaatcaat gcctttcatt 480
gaagcattag gtcaattttc ataccgtgta gggcctggaa acttctgctt ggtgcatgtc 540
agtcttgtac cagttcttaa tgtagttggt gagcagaaaa caaagcccac ccaacatagt 600
gttcgcggac ttagaggact tggactcatg cctgatattt tggcatgtcg tagtacacat 660
ccacttgaag aacatgtgaa agtgaagctc gcgcaatttt gtcatgttcc gataccaaat 720
atcattaatc tccatgatgt cacaaacatt tggcacatcc ctttgttgct cagagatcag 780
aaggcccatg aagctatttt gaaagtgtta gaccttcagt gtgtgggtaa agttgctcga 840
gaaccccaat tgtctgaatg gactgaaaga gccagcagat gtgacagatt gaaaactccg 900
gttaggattg ctatggttgg aaagtatact ggcttatcag attcctacct atctgttatc 960
aaggctcttt tgcatgcgtc agttgctttg gacaggaaac ttgtggtgga ctgggttcct 1020
tcctgtgatc ttgaagattc tacagcagaa gagactcctg atgcctatga aaaagcatgg 1080
gagtcgctaa agggtgcaga tggtgtgttg gtgccaggag gttttggaga cagaggagtc 1140
caagggaaaa ttcttgctgc aaaatacgcg cgagaaaaca acattccata tcttggcatt 1200
tgcttgggca tgcaaattgc agtgattgag tttgcacgtt ctatcatgaa gttgcatggt 1260
gctaacagca cagagtttga tccaaccaca aaaacaccat gtgttatttt catgccagag 1320
ggatccaaaa cccatatggg ggcaacaatg cgccttggat caaggaggac ctttttccag 1380
gtcactaact gcaaatctgc taaactgtat ggcaatgtta gctacattga tgaaaggcac 1440
cgccacagat acgaggtgaa tcctgatatg gtcccagaat ttgagaaggc agggctttct 1500
ttcgttggca gggatgaaag cggtaaacgc atggagatta tcgaactacc aactcatagg 1560
tttttcgttg gtgtacaatt tcaccctgaa ttcaagtcaa ggcctggcaa cccatctccg 1620
cttttcttag gattggtagc agcatcatca ggtcagcttg atcaacgtgg cgttatcagt 1680
aagacaagca gatgcatcag caataccgga ggagcatcaa agctaaaagc ttgccagaat 1740
gggcacctca tgaagccact gaagggcctg gtgaacgggc actattcaac aaccagcaat 1800
ggcgccattc ccatctag 1818
<210> 78
<211> 605
<212> PRT
<213> Sorghum bicolor
<400> 78
Met Lys Tyr Val Leu Val Thr Gly Gly Val Val Ser Gly Leu Gly Lys
1 5 10 15
Gly Val Thr Ala Ser Ser Ile Gly Val Leu Leu Lys Ser Cys Gly Phe
20 25 30
Arg Val Thr Ser Ile Lys Ile Asp Pro Tyr Leu Asn Thr Asp Ala Gly
35 40 45
Thr Met Ser Pro Phe Glu His Gly Glu Val Phe Val Leu Asp Asp Gly
50 55 60
Gly Glu Val Asp Leu Asp Leu Gly Asn Tyr Glu Arg Phe Leu Asp Ile
65 70 75 80
Lys Leu Thr Arg Asp Asn Asn Ile Thr Thr Gly Lys Ile Tyr Gln Ser
85 90 95
Val Ile Asp Lys Glu Arg Arg Gly Asp Tyr Leu Gly Lys Thr Val Gln
100 105 110
Val Val Pro His Ile Thr Asp Glu Ile Gln Glu Trp Ile Glu Arg Val
115 120 125
Ala Met Asn Pro Val Asp Gly Thr Glu Glu Ala Ala Asp Val Cys Val
130 135 140
Ile Glu Leu Gly Gly Thr Ile Gly Asp Ile Glu Ser Met Pro Phe Ile
145 150 155 160
Glu Ala Leu Gly Gln Phe Ser Tyr Arg Val Gly Pro Gly Asn Phe Cys
165 170 175
Leu Val His Val Ser Leu Val Pro Val Leu Asn Val Val Gly Glu Gln
180 185 190
Lys Thr Lys Pro Thr Gln His Ser Val Arg Gly Leu Arg Gly Leu Gly
195 200 205
Leu Met Pro Asp Ile Leu Ala Cys Arg Ser Thr His Pro Leu Glu Glu
210 215 220
His Val Lys Val Lys Leu Ala Gln Phe Cys His Val Pro Ile Pro Asn
225 230 235 240
Ile Ile Asn Leu His Asp Val Thr Asn Ile Trp His Ile Pro Leu Leu
245 250 255
Leu Arg Asp Gln Lys Ala His Glu Ala Ile Leu Lys Val Leu Asp Leu
260 265 270
Gln Cys Val Gly Lys Val Ala Arg Glu Pro Gln Leu Ser Glu Trp Thr
275 280 285
Glu Arg Ala Ser Arg Cys Asp Arg Leu Lys Thr Pro Val Arg Ile Ala
290 295 300
Met Val Gly Lys Tyr Thr Gly Leu Ser Asp Ser Tyr Leu Ser Val Ile
305 310 315 320
Lys Ala Leu Leu His Ala Ser Val Ala Leu Asp Arg Lys Leu Val Val
325 330 335
Asp Trp Val Pro Ser Cys Asp Leu Glu Asp Ser Thr Ala Glu Glu Thr
340 345 350
Pro Asp Ala Tyr Glu Lys Ala Trp Glu Ser Leu Lys Gly Ala Asp Gly
355 360 365
Val Leu Val Pro Gly Gly Phe Gly Asp Arg Gly Val Gln Gly Lys Ile
370 375 380
Leu Ala Ala Lys Tyr Ala Arg Glu Asn Asn Ile Pro Tyr Leu Gly Ile
385 390 395 400
Cys Leu Gly Met Gln Ile Ala Val Ile Glu Phe Ala Arg Ser Ile Met
405 410 415
Lys Leu His Gly Ala Asn Ser Thr Glu Phe Asp Pro Thr Thr Lys Thr
420 425 430
Pro Cys Val Ile Phe Met Pro Glu Gly Ser Lys Thr His Met Gly Ala
435 440 445
Thr Met Arg Leu Gly Ser Arg Arg Thr Phe Phe Gln Val Thr Asn Cys
450 455 460
Lys Ser Ala Lys Leu Tyr Gly Asn Val Ser Tyr Ile Asp Glu Arg His
465 470 475 480
Arg His Arg Tyr Glu Val Asn Pro Asp Met Val Pro Glu Phe Glu Lys
485 490 495
Ala Gly Leu Ser Phe Val Gly Arg Asp Glu Ser Gly Lys Arg Met Glu
500 505 510
Ile Ile Glu Leu Pro Thr His Arg Phe Phe Val Gly Val Gln Phe His
515 520 525
Pro Glu Phe Lys Ser Arg Pro Gly Asn Pro Ser Pro Leu Phe Leu Gly
530 535 540
Leu Val Ala Ala Ser Ser Gly Gln Leu Asp Gln Arg Gly Val Ile Ser
545 550 555 560
Lys Thr Ser Arg Cys Ile Ser Asn Thr Gly Gly Ala Ser Lys Leu Lys
565 570 575
Ala Cys Gln Asn Gly His Leu Met Lys Pro Leu Lys Gly Leu Val Asn
580 585 590
Gly His Tyr Ser Thr Thr Ser Asn Gly Ala Ile Pro Ile
595 600 605
<210> 79
<211> 1776
<212> DNA
<213> Arabidopsis thaliana
<400> 79
atgaagtacg ttttggtgac aggaggagtg gtgagtggtc ttggcaaagg tgtcactgct 60
agtagcattg gacttctcct tcaagcatgt ggtcttcgtg tcacttccat taaaattgat 120
ccttatctta acactgatgc tggaacaatg tctccgtttg agcatggaga agtatttgta 180
ttggatgatg gtggagaggt ggatttggac cttggaaact atgaacgatt tttagacagt 240
acattgaccc gtgacaacaa tataactact ggaaagatat accagtcagt tattgacaag 300
gaaaggaagg gggactacct cggaagaact gtacaggttg ttcctcatgt tactgacgca 360
atccaagagt ggattgagcg tgtagctaat gttcctgtgg atggaaagga aggtcctcct 420
gatgtttgtg tcatcgaatt aggcgggact ataggtgata ttgaatctat gccctttatt 480
gaggcccttg gtcaattctc gtataaagtt ggacctggta atttctgctt ggttcatgtc 540
agccttgtgc ctgttctcag tgttgttggt gaacagaaga cgaagccaac ccagcatagt 600
gtgcgaggcc tcaggagcct tggtttgaca ccaaatatcc tagcatgtcg cagcacgaag 660
gcacttgaag aaaatgtcaa gacaaaacta tctcaatttt gccatgtgcc ggaagtgaat 720
atagtaacgc tctatgatgt tccaaatatt tggcacgttc ctctgctttt aagggatcaa 780
aaagctcatg aagcgatcct aagagagtta aatcttagta atgctataaa gcctgacttg 840
acagaatgga ctgcaaggac taaaatttat gacacactgc aagaccctgt gagaatagct 900
atggttggaa agtacactgg ccttactgat tcttaccttt ccgtgttgaa ggcccttttg 960
catgcttctg ttgcatgtca caagaagctt gtcatagagt gggttgcagc tagtgacctt 1020
gaagagataa ctgcacaaga gacgccagat gtccataagg ctgcatggga tcttttgaag 1080
ggtgctgatg gtatcctagt accaggaggg tttggtgata gaggagtgca agggaagata 1140
cttgctacaa agtacgcccg tgaaaatcaa gtccctttcc ttggcatatg cctgggaatg 1200
cagctggctg ttgttgaatt tgcccgctcc attcttggtt ttcacgatgc aaacagcaca 1260
gagtttgaac cagaaacttc aagcccttgc atcatattta tgccagaagg atccacaact 1320
catatggggg gcacaatgcg cttagggtca aggaagactt acttccaggt tgctgattgc 1380
aagtctgcca agctgtacgg taatgcaaag tttgtagatg agcgacacag gcacagatat 1440
gaggtaaatc cagatatgat atcagaaatt gagaaggctg gcctctcctt tgttgggaaa 1500
gatgagactg ggcgtcgtat ggagattgtt gaactaccaa gtcatccata ctttgtgggt 1560
gctcagttcc atcctgaatt caagtccaga cctgggaaac cttctgcatt gtttctaggt 1620
cttatcgcag cagcgtctgg gtgtctagaa tcagtattgc aaacaggtgg caaggtgaac 1680
atagtttcga aaaatggagt agccaatgga tctgcaatgg ggaaagttca tcagaacggc 1740
aatgtctata gcaatggaaa cgggcttcac cactga 1776
<210> 80
<211> 591
<212> PRT
<213> Arabidopsis thaliana
<400> 80
Met Lys Tyr Val Leu Val Thr Gly Gly Val Val Ser Gly Leu Gly Lys
1 5 10 15
Gly Val Thr Ala Ser Ser Ile Gly Leu Leu Leu Gln Ala Cys Gly Leu
20 25 30
Arg Val Thr Ser Ile Lys Ile Asp Pro Tyr Leu Asn Thr Asp Ala Gly
35 40 45
Thr Met Ser Pro Phe Glu His Gly Glu Val Phe Val Leu Asp Asp Gly
50 55 60
Gly Glu Val Asp Leu Asp Leu Gly Asn Tyr Glu Arg Phe Leu Asp Ser
65 70 75 80
Thr Leu Thr Arg Asp Asn Asn Ile Thr Thr Gly Lys Ile Tyr Gln Ser
85 90 95
Val Ile Asp Lys Glu Arg Lys Gly Asp Tyr Leu Gly Arg Thr Val Gln
100 105 110
Val Val Pro His Val Thr Asp Ala Ile Gln Glu Trp Ile Glu Arg Val
115 120 125
Ala Asn Val Pro Val Asp Gly Lys Glu Gly Pro Pro Asp Val Cys Val
130 135 140
Ile Glu Leu Gly Gly Thr Ile Gly Asp Ile Glu Ser Met Pro Phe Ile
145 150 155 160
Glu Ala Leu Gly Gln Phe Ser Tyr Lys Val Gly Pro Gly Asn Phe Cys
165 170 175
Leu Val His Val Ser Leu Val Pro Val Leu Ser Val Val Gly Glu Gln
180 185 190
Lys Thr Lys Pro Thr Gln His Ser Val Arg Gly Leu Arg Ser Leu Gly
195 200 205
Leu Thr Pro Asn Ile Leu Ala Cys Arg Ser Thr Lys Ala Leu Glu Glu
210 215 220
Asn Val Lys Thr Lys Leu Ser Gln Phe Cys His Val Pro Glu Val Asn
225 230 235 240
Ile Val Thr Leu Tyr Asp Val Pro Asn Ile Trp His Val Pro Leu Leu
245 250 255
Leu Arg Asp Gln Lys Ala His Glu Ala Ile Leu Arg Glu Leu Asn Leu
260 265 270
Ser Asn Ala Ile Lys Pro Asp Leu Thr Glu Trp Thr Ala Arg Thr Lys
275 280 285
Ile Tyr Asp Thr Leu Gln Asp Pro Val Arg Ile Ala Met Val Gly Lys
290 295 300
Tyr Thr Gly Leu Thr Asp Ser Tyr Leu Ser Val Leu Lys Ala Leu Leu
305 310 315 320
His Ala Ser Val Ala Cys His Lys Lys Leu Val Ile Glu Trp Val Ala
325 330 335
Ala Ser Asp Leu Glu Glu Ile Thr Ala Gln Glu Thr Pro Asp Val His
340 345 350
Lys Ala Ala Trp Asp Leu Leu Lys Gly Ala Asp Gly Ile Leu Val Pro
355 360 365
Gly Gly Phe Gly Asp Arg Gly Val Gln Gly Lys Ile Leu Ala Thr Lys
370 375 380
Tyr Ala Arg Glu Asn Gln Val Pro Phe Leu Gly Ile Cys Leu Gly Met
385 390 395 400
Gln Leu Ala Val Val Glu Phe Ala Arg Ser Ile Leu Gly Phe His Asp
405 410 415
Ala Asn Ser Thr Glu Phe Glu Pro Glu Thr Ser Ser Pro Cys Ile Ile
420 425 430
Phe Met Pro Glu Gly Ser Thr Thr His Met Gly Gly Thr Met Arg Leu
435 440 445
Gly Ser Arg Lys Thr Tyr Phe Gln Val Ala Asp Cys Lys Ser Ala Lys
450 455 460
Leu Tyr Gly Asn Ala Lys Phe Val Asp Glu Arg His Arg His Arg Tyr
465 470 475 480
Glu Val Asn Pro Asp Met Ile Ser Glu Ile Glu Lys Ala Gly Leu Ser
485 490 495
Phe Val Gly Lys Asp Glu Thr Gly Arg Arg Met Glu Ile Val Glu Leu
500 505 510
Pro Ser His Pro Tyr Phe Val Gly Ala Gln Phe His Pro Glu Phe Lys
515 520 525
Ser Arg Pro Gly Lys Pro Ser Ala Leu Phe Leu Gly Leu Ile Ala Ala
530 535 540
Ala Ser Gly Cys Leu Glu Ser Val Leu Gln Thr Gly Gly Lys Val Asn
545 550 555 560
Ile Val Ser Lys Asn Gly Val Ala Asn Gly Ser Ala Met Gly Lys Val
565 570 575
His Gln Asn Gly Asn Val Tyr Ser Asn Gly Asn Gly Leu His His
580 585 590
<210> 81
<211> 1911
<212> DNA
<213> Glycine max
<400> 81
atgaagtacc tgttggtgac gggtggagtt gtgagtggac ttgggaaagg agtcactgca 60
agcagtattg gagtactcct tcaggcttgt ggctttcggg ttacttctat caagattgat 120
ccctatctga acactgatgc agggacaatg tctccttttg agcatgggga agtgtttgtt 180
ttagatgatg gcggtgaggt tgaccttgat cttggaaact atgaacgttt cttggacctc 240
aaattaactc gtgacaataa tatcacaact gggaaaatat atcagtctgt aattgaaaag 300
gagagaagag gagattatct tggcaagact gtgcaggttg ttccacacat tactgatgcc 360
atccaagaat ggatagagcg tgtggcacag ataccagttg atggaaaaga aggcccagct 420
gatgtttgtg ttattgagtt gggtggaact attggagata tcgagtctat gccttttatt 480
gaagcacttg gccagttttc ataccgtgtt ggccctggta acttctgttt agttcatgtc 540
agcttggtgc ctgttatcaa tgttgttggt gagcagaaaa caaagccaac ccaacacagt 600
gtccgccaac ttagagggtt agggttgacc ccaaatcttc ttgcttgtcg cagttcaaag 660
gaacttgatg acaacattaa ggaaaaactt tctcaatttt gtcatgttcc gtcatcaagc 720
atactaactc tctatgatgt tccaaatatt tggcacattc ctttgctatt aagtgaccaa 780
aaggcgcatg atgcaatcct gaaaacatta aacctgcgag gtgttgctac agagcctaat 840
tttaaggagt ggattacaac aacaaaagta tatgacaaat ttcatgaaat ggtaagtttc 900
aatttttcta atatatcatc taaaaaagct aatatatttc atgaatctgt gagttccaat 960
attcaactct ctatagttag aattgcaatg gtgggaaaat atactaacct ctcagatgca 1020
tatctttctg tactgaaggc acttttgcat gcttctgttg cttgcaatca tgagcttgtt 1080
gtggatttgg ttcccgctga acatctcgaa gatgatacct ctaaagagga tcctgatgca 1140
tataaagctg cttggggtct tttgaaggga gctaatggga ttctagttcc aggaggtttt 1200
ggtgacagag gagtggaagg aaaaattctt gctgccaagt atgctcgaga aaatagcatt 1260
ccatatctgg gcatttgctt ggggatgcaa ataggtgtaa ttgagttttc aagatctgtt 1320
ctgggtcttc atgatgctaa tagcacagaa tttgatccca aaaccaaaaa cccttgtgtc 1380
atatttatgc cagaaggttc aaagactcat atggggggaa ctatgcgtct tggttcaagg 1440
agaacttact ttcatgttgc tgactgcaaa tcagccaagt tgtatggtaa tgcaagcttt 1500
gttgatgagc gacatcggca tagatacgag gttaatcctg atatgatatc acaactcgag 1560
agtgctggtc tatcttttgt tggcaaagac gaaactggga agcgcatgga gatagttgaa 1620
tttcctggtc atcctttctt cattggtgct cagtttcatc ctgagttcaa gtccagacca 1680
gggaaacctt ctccattatt cttaggatta atatcagcag catgtgagag gacagttgtg 1740
cctgcaagca aaggttatgg caagttaaca aatgggatac actcaccaat gttgaaagca 1800
gcagcagcac ataatggaaa taatgggttc aaatcttcca atagttcctt aaatggtgta 1860
tatacaagta caactaccaa tggggtgtgt gttgatggga ggagctgtta a 1911
<210> 82
<211> 636
<212> PRT
<213> Glycine max
<400> 82
Met Lys Tyr Leu Leu Val Thr Gly Gly Val Val Ser Gly Leu Gly Lys
1 5 10 15
Gly Val Thr Ala Ser Ser Ile Gly Val Leu Leu Gln Ala Cys Gly Phe
20 25 30
Arg Val Thr Ser Ile Lys Ile Asp Pro Tyr Leu Asn Thr Asp Ala Gly
35 40 45
Thr Met Ser Pro Phe Glu His Gly Glu Val Phe Val Leu Asp Asp Gly
50 55 60
Gly Glu Val Asp Leu Asp Leu Gly Asn Tyr Glu Arg Phe Leu Asp Leu
65 70 75 80
Lys Leu Thr Arg Asp Asn Asn Ile Thr Thr Gly Lys Ile Tyr Gln Ser
85 90 95
Val Ile Glu Lys Glu Arg Arg Gly Asp Tyr Leu Gly Lys Thr Val Gln
100 105 110
Val Val Pro His Ile Thr Asp Ala Ile Gln Glu Trp Ile Glu Arg Val
115 120 125
Ala Gln Ile Pro Val Asp Gly Lys Glu Gly Pro Ala Asp Val Cys Val
130 135 140
Ile Glu Leu Gly Gly Thr Ile Gly Asp Ile Glu Ser Met Pro Phe Ile
145 150 155 160
Glu Ala Leu Gly Gln Phe Ser Tyr Arg Val Gly Pro Gly Asn Phe Cys
165 170 175
Leu Val His Val Ser Leu Val Pro Val Ile Asn Val Val Gly Glu Gln
180 185 190
Lys Thr Lys Pro Thr Gln His Ser Val Arg Gln Leu Arg Gly Leu Gly
195 200 205
Leu Thr Pro Asn Leu Leu Ala Cys Arg Ser Ser Lys Glu Leu Asp Asp
210 215 220
Asn Ile Lys Glu Lys Leu Ser Gln Phe Cys His Val Pro Ser Ser Ser
225 230 235 240
Ile Leu Thr Leu Tyr Asp Val Pro Asn Ile Trp His Ile Pro Leu Leu
245 250 255
Leu Ser Asp Gln Lys Ala His Asp Ala Ile Leu Lys Thr Leu Asn Leu
260 265 270
Arg Gly Val Ala Thr Glu Pro Asn Phe Lys Glu Trp Ile Thr Thr Thr
275 280 285
Lys Val Tyr Asp Lys Phe His Glu Met Val Ser Phe Asn Phe Ser Asn
290 295 300
Ile Ser Ser Lys Lys Ala Asn Ile Phe His Glu Ser Val Ser Ser Asn
305 310 315 320
Ile Gln Leu Ser Ile Val Arg Ile Ala Met Val Gly Lys Tyr Thr Asn
325 330 335
Leu Ser Asp Ala Tyr Leu Ser Val Leu Lys Ala Leu Leu His Ala Ser
340 345 350
Val Ala Cys Asn His Glu Leu Val Val Asp Leu Val Pro Ala Glu His
355 360 365
Leu Glu Asp Asp Thr Ser Lys Glu Asp Pro Asp Ala Tyr Lys Ala Ala
370 375 380
Trp Gly Leu Leu Lys Gly Ala Asn Gly Ile Leu Val Pro Gly Gly Phe
385 390 395 400
Gly Asp Arg Gly Val Glu Gly Lys Ile Leu Ala Ala Lys Tyr Ala Arg
405 410 415
Glu Asn Ser Ile Pro Tyr Leu Gly Ile Cys Leu Gly Met Gln Ile Gly
420 425 430
Val Ile Glu Phe Ser Arg Ser Val Leu Gly Leu His Asp Ala Asn Ser
435 440 445
Thr Glu Phe Asp Pro Lys Thr Lys Asn Pro Cys Val Ile Phe Met Pro
450 455 460
Glu Gly Ser Lys Thr His Met Gly Gly Thr Met Arg Leu Gly Ser Arg
465 470 475 480
Arg Thr Tyr Phe His Val Ala Asp Cys Lys Ser Ala Lys Leu Tyr Gly
485 490 495
Asn Ala Ser Phe Val Asp Glu Arg His Arg His Arg Tyr Glu Val Asn
500 505 510
Pro Asp Met Ile Ser Gln Leu Glu Ser Ala Gly Leu Ser Phe Val Gly
515 520 525
Lys Asp Glu Thr Gly Lys Arg Met Glu Ile Val Glu Phe Pro Gly His
530 535 540
Pro Phe Phe Ile Gly Ala Gln Phe His Pro Glu Phe Lys Ser Arg Pro
545 550 555 560
Gly Lys Pro Ser Pro Leu Phe Leu Gly Leu Ile Ser Ala Ala Cys Glu
565 570 575
Arg Thr Val Val Pro Ala Ser Lys Gly Tyr Gly Lys Leu Thr Asn Gly
580 585 590
Ile His Ser Pro Met Leu Lys Ala Ala Ala Ala His Asn Gly Asn Asn
595 600 605
Gly Phe Lys Ser Ser Asn Ser Ser Leu Asn Gly Val Tyr Thr Ser Thr
610 615 620
Thr Thr Asn Gly Val Cys Val Asp Gly Arg Ser Cys
625 630 635
<210> 83
<211> 1164
<212> DNA
<213> Oryza sativa
<400> 83
atgtcgtcgc agcaatggct tggtgacggc acggcgcgga ggtggaggga gctccatggt 60
gagagcgact gggacggcct cctggacccg ttcgacctcg acctccgccg caccgtcatc 120
cgctacggcg agatggcgca ggcgacgtac gacgccttca accacgagaa gctctcgccg 180
cacgcgggcc tctcgaggtt cgccgcgcgc cgcttcttcg agcgggcgca gctgccgggc 240
cactccgcgg cgtaccgcgt cgccaggtgc agggagagca actggatcgg gtacgtcgcg 300
gtggccaccg acgaagggaa ggctgcgctc gggcgccgcg acatcgtcgt cgcgtggcgc 360
ggcacggtgc agtcgctgga gtggatcaag gacatggact tcgtcatggt gccacccaag 420
ggcctcctcc gggacaaagc ttccgacgcc atggtgcatc gagggtggct gtccatgtac 480
acctccaggg actctgagtc cagccacaac aaggacagtg ctcgagatca ggtgttgagc 540
gaggtggcga agctggtgag catgtaccag gacgaggaac tgagcatcac ggtgacggga 600
cacagcctcg gcgccgcact cgcgacgctg aacgcgttcg acatcgtcga gaacgggtac 660
aacagggcac cccgcgccgc ggcagcggcg gcgggctgcc cggtcaccgc gttcgtgttc 720
gccagcccgc gcgtcggcgg gcacggcttc aagcgccgct tcgacggcgc gcgtggcctc 780
ggcctccgcc tcctccgcgt ccgcaacgcg cgcgacgtcg tccccaggta cccgccggcg 840
ccgccgtacc acggcgtggg cacagagctg gcgatcgaca cgggcgagtc gccgtacctg 900
aggaggcccg ggaacgagct ggtgtggcac aacctcgagt gctacctgca cggcgtggcc 960
ggcgcgcgcg gcggcgaggc cgggcggttc aagctcgccg tggagcgcga cgtggcgctg 1020
gcgaacaagt cctacggcgc gctgcgcgac gagcacgccg tgccggccgg gtggtggatc 1080
ccgtcgaaca ggggcatggt gagaggcgcc gatggccgct ggactctgat ggaccgcgag 1140
gaagatgagg atagtgcaga gtaa 1164
<210> 84
<211> 387
<212> PRT
<213> Oryza sativa
<400> 84
Met Ser Ser Gln Gln Trp Leu Gly Asp Gly Thr Ala Arg Arg Trp Arg
1 5 10 15
Glu Leu His Gly Glu Ser Asp Trp Asp Gly Leu Leu Asp Pro Phe Asp
20 25 30
Leu Asp Leu Arg Arg Thr Val Ile Arg Tyr Gly Glu Met Ala Gln Ala
35 40 45
Thr Tyr Asp Ala Phe Asn His Glu Lys Leu Ser Pro His Ala Gly Leu
50 55 60
Ser Arg Phe Ala Ala Arg Arg Phe Phe Glu Arg Ala Gln Leu Pro Gly
65 70 75 80
His Ser Ala Ala Tyr Arg Val Ala Arg Cys Arg Glu Ser Asn Trp Ile
85 90 95
Gly Tyr Val Ala Val Ala Thr Asp Glu Gly Lys Ala Ala Leu Gly Arg
100 105 110
Arg Asp Ile Val Val Ala Trp Arg Gly Thr Val Gln Ser Leu Glu Trp
115 120 125
Ile Lys Asp Met Asp Phe Val Met Val Pro Pro Lys Gly Leu Leu Arg
130 135 140
Asp Lys Ala Ser Asp Ala Met Val His Arg Gly Trp Leu Ser Met Tyr
145 150 155 160
Thr Ser Arg Asp Ser Glu Ser Ser His Asn Lys Asp Ser Ala Arg Asp
165 170 175
Gln Val Leu Ser Glu Val Ala Lys Leu Val Ser Met Tyr Gln Asp Glu
180 185 190
Glu Leu Ser Ile Thr Val Thr Gly His Ser Leu Gly Ala Ala Leu Ala
195 200 205
Thr Leu Asn Ala Phe Asp Ile Val Glu Asn Gly Tyr Asn Arg Ala Pro
210 215 220
Arg Ala Ala Ala Ala Ala Ala Gly Cys Pro Val Thr Ala Phe Val Phe
225 230 235 240
Ala Ser Pro Arg Val Gly Gly His Gly Phe Lys Arg Arg Phe Asp Gly
245 250 255
Ala Arg Gly Leu Gly Leu Arg Leu Leu Arg Val Arg Asn Ala Arg Asp
260 265 270
Val Val Pro Arg Tyr Pro Pro Ala Pro Pro Tyr His Gly Val Gly Thr
275 280 285
Glu Leu Ala Ile Asp Thr Gly Glu Ser Pro Tyr Leu Arg Arg Pro Gly
290 295 300
Asn Glu Leu Val Trp His Asn Leu Glu Cys Tyr Leu His Gly Val Ala
305 310 315 320
Gly Ala Arg Gly Gly Glu Ala Gly Arg Phe Lys Leu Ala Val Glu Arg
325 330 335
Asp Val Ala Leu Ala Asn Lys Ser Tyr Gly Ala Leu Arg Asp Glu His
340 345 350
Ala Val Pro Ala Gly Trp Trp Ile Pro Ser Asn Arg Gly Met Val Arg
355 360 365
Gly Ala Asp Gly Arg Trp Thr Leu Met Asp Arg Glu Glu Asp Glu Asp
370 375 380
Ser Ala Glu
385
<210> 85
<211> 1263
<212> DNA
<213> Zea mays
<400> 85
atgcagctgc gcaccagaca tgtccgttcc atgacccagt cccagcacga tccatgtcat 60
gcctttgccg tttgcaatct tcgtcgtcag aatcagaagc aaacccccga gcaagctacc 120
gtcactgtgt acaccagaag aagcaccagc aaggcaactg cgagcgagct gcgttacgtg 180
cccagcaaca tgtcccagca cggcggcctc ggggacacgg cccggcggtg gcgcgagctc 240
cacggcggcg acaacagctg gacggggctg ctggacccgc tggacctcga cctgcgccgc 300
accgtgctcc gctacggcga gatggcgcag gccacctacg acgccttcaa ctgcgagcgc 360
gcgtcgccgc acgcggggct ctcccgcttc gccagggcgc gcttcttcga ccgggcgcgg 420
ctgccggcgc acgccgccgc gtaccgggtc accaggttcc tgtacgccac gtcgtcggta 480
gccgtgccgg ccgccttcat gctctggtcc gtggccgggt cgcgccggcg gtgcagggag 540
tctaactgga tcggctacgt cgcggcggcc accgacgagg ggaaagccgc gctcgggcgc 600
cgcgacatcg tcgtcgcgtg gcgcggcacc gtggaggcgc tggagtgggc cgacgcccga 660
cgacctcgag ttccccatgg tgccgacgga aggccgcctc ggggacggcg acgcctgcga 720
cgccatggtg caccgcggct ggctgtccat gtacacctcc gccgaccccg cgtccagcca 780
caaccaggac agcgcgcggg accaggcgct gggcgaggtg cggaggctgg tggacgcgta 840
caaggacgag gagctcagcg tcaccgtgac gggccacagc ctcggcgcgg cgctcgccac 900
gctcaacgcg ttcgacatcg ccgccaacgg ctacaacgtg gcggcgacgg cgacggccgc 960
ctgccccgtg accgcgttcg cgttcgccag cccgcgcgtc ggcggcgctg gcttcaggaa 1020
gcggttcgac gctgtcccgg ggctgcggct cctccgcgtc cgcaacgccc gcgacgtcgt 1080
gcccaagtac ccggccgtgt tctaccacga cgtcggcgcg gagctcgcgg tcgacacggg 1140
ggagtcgccg tacctgagga gccccgggcg cgagcggacc tgccacaacc tcgaggtgta 1200
cctgcacggc gtggcgggca cgcgaggcgc gcgcggaggg ttcgagctcg ccgtggcgcg 1260
tga 1263
<210> 86
<211> 420
<212> PRT
<213> Zea mays
<400> 86
Met Gln Leu Arg Thr Arg His Val Arg Ser Met Thr Gln Ser Gln His
1 5 10 15
Asp Pro Cys His Ala Phe Ala Val Cys Asn Leu Arg Arg Gln Asn Gln
20 25 30
Lys Gln Thr Pro Glu Gln Ala Thr Val Thr Val Tyr Thr Arg Arg Ser
35 40 45
Thr Ser Lys Ala Thr Ala Ser Glu Leu Arg Tyr Val Pro Ser Asn Met
50 55 60
Ser Gln His Gly Gly Leu Gly Asp Thr Ala Arg Arg Trp Arg Glu Leu
65 70 75 80
His Gly Gly Asp Asn Ser Trp Thr Gly Leu Leu Asp Pro Leu Asp Leu
85 90 95
Asp Leu Arg Arg Thr Val Leu Arg Tyr Gly Glu Met Ala Gln Ala Thr
100 105 110
Tyr Asp Ala Phe Asn Cys Glu Arg Ala Ser Pro His Ala Gly Leu Ser
115 120 125
Arg Phe Ala Arg Ala Arg Phe Phe Asp Arg Ala Arg Leu Pro Ala His
130 135 140
Ala Ala Ala Tyr Arg Val Thr Arg Phe Leu Tyr Ala Thr Ser Ser Val
145 150 155 160
Ala Val Pro Ala Ala Phe Met Leu Trp Ser Val Ala Gly Ser Arg Arg
165 170 175
Arg Cys Arg Glu Ser Asn Trp Ile Gly Tyr Val Ala Ala Ala Thr Asp
180 185 190
Glu Gly Lys Ala Ala Leu Gly Arg Arg Asp Ile Val Val Ala Trp Arg
195 200 205
Gly Thr Val Glu Ala Leu Glu Trp Ala Asp Ala Arg Arg Pro Arg Val
210 215 220
Pro His Gly Ala Asp Gly Arg Pro Pro Arg Gly Arg Arg Arg Leu Arg
225 230 235 240
Arg His Gly Ala Pro Arg Leu Ala Val His Val His Leu Arg Arg Pro
245 250 255
Arg Val Gln Pro Gln Pro Gly Gln Arg Ala Gly Pro Gly Ala Gly Arg
260 265 270
Gly Ala Glu Ala Gly Gly Arg Val Gln Gly Arg Gly Ala Gln Arg His
275 280 285
Arg Asp Gly Pro Gln Pro Arg Arg Gly Ala Arg His Ala Gln Arg Val
290 295 300
Arg His Arg Arg Gln Arg Leu Gln Arg Gly Gly Asp Gly Asp Gly Arg
305 310 315 320
Leu Pro Arg Asp Arg Val Arg Val Arg Gln Pro Ala Arg Arg Arg Arg
325 330 335
Trp Leu Gln Glu Ala Val Arg Arg Cys Pro Gly Ala Ala Ala Pro Pro
340 345 350
Arg Pro Gln Arg Pro Arg Arg Arg Ala Gln Val Pro Gly Arg Val Leu
355 360 365
Pro Arg Arg Arg Arg Gly Ala Arg Gly Arg His Gly Gly Val Ala Val
370 375 380
Pro Glu Glu Pro Arg Ala Arg Ala Asp Leu Pro Gln Pro Arg Gly Val
385 390 395 400
Pro Ala Arg Arg Gly Gly His Ala Arg Arg Ala Arg Arg Val Arg Ala
405 410 415
Arg Arg Gly Ala
420
<210> 87
<211> 1227
<212> DNA
<213> Sorghum bicolor
<400> 87
atgatgtcca accacggcgg cctcggcgac acggcccggc ggtggcgcga gctccacggc 60
gtcggcggcg acaacagcgg ctgggcgggg ctgctggacc cgctggacct cgacctgcgc 120
cgcaccgtgc tccgctacgg cgagatggcg caggcgacct acgacgcctt caaccgcgag 180
cgttcgtcgc cgcacgcggg tctgtcccgc ttcgccaggg cgcgcttctt cgaccgggtg 240
cggctgccgg cgcacgccgc cgcgtaccgg gtcaccaggt tcctgtacgc cacgtcgtcg 300
gtggctttgc cggacgcctt catgctgagg tccgtgtcca ggtcgcgccg gtgcagggag 360
tccaactgga tcggctacgt cgcggtggcc accgacgaag ggaaggccgc gctgggccgc 420
cgcgacgtcg tcgtcgtgtg gcgcggcacc atgcagaagc tggagtgggc cgacgacctc 480
gagttcccca tggtgtcgac caaaggcctc ctcggcgatg gccaggccgc ctgcgatgcc 540
atggtgcacc gtggctggct gtccatgtac acctccatcg acccggcgtc cagccacaac 600
caggacagcg cgcggcacca ggcgctgagc gaggtgcgga ggctggtgga cgcgtacagc 660
gacgaggagc gcagcatcac cgtcgtggga cacagcctcg gcgcggcgct cgccacgctc 720
aacgcgttcg acatcgccgc caacggctac aacgtggcga cgggcgcggc ggcctgcccg 780
gtgaccgcgt tcgcgttcgc cagcccgcgc gtcggcggcg gcggcttcaa gaagcggttc 840
gacgccgtgc cggggctgcg tctcctgcgc gtccggaacg cccgcgacgt cgtgcccaag 900
tacccgatcg tgttctacca cgacgtcggc gcggagctcg cgatcgacac gggggagtcg 960
ccgtacctga ggagccccgg gcgcgagcat acctggcaca acctcgaggt gtacctgcac 1020
ggcgtggcgg gcacgcgggg cgcgcgcgga gggttcgagc tcgccgtggc gcgggacgtg 1080
gcgctggtga acaagctata cgacgtgctg tgggacgact acggggtgcc gcctgggtgg 1140
tgggtgccgc tgaacaaggg catggtggag ggcgccgacg ggcgctggag cttgatggac 1200
tgcgaggagg acgaggaaga tgagtag 1227
<210> 88
<211> 408
<212> PRT
<213> Sorghum bicolor
<400> 88
Met Met Ser Asn His Gly Gly Leu Gly Asp Thr Ala Arg Arg Trp Arg
1 5 10 15
Glu Leu His Gly Val Gly Gly Asp Asn Ser Gly Trp Ala Gly Leu Leu
20 25 30
Asp Pro Leu Asp Leu Asp Leu Arg Arg Thr Val Leu Arg Tyr Gly Glu
35 40 45
Met Ala Gln Ala Thr Tyr Asp Ala Phe Asn Arg Glu Arg Ser Ser Pro
50 55 60
His Ala Gly Leu Ser Arg Phe Ala Arg Ala Arg Phe Phe Asp Arg Val
65 70 75 80
Arg Leu Pro Ala His Ala Ala Ala Tyr Arg Val Thr Arg Phe Leu Tyr
85 90 95
Ala Thr Ser Ser Val Ala Leu Pro Asp Ala Phe Met Leu Arg Ser Val
100 105 110
Ser Arg Ser Arg Arg Cys Arg Glu Ser Asn Trp Ile Gly Tyr Val Ala
115 120 125
Val Ala Thr Asp Glu Gly Lys Ala Ala Leu Gly Arg Arg Asp Val Val
130 135 140
Val Val Trp Arg Gly Thr Met Gln Lys Leu Glu Trp Ala Asp Asp Leu
145 150 155 160
Glu Phe Pro Met Val Ser Thr Lys Gly Leu Leu Gly Asp Gly Gln Ala
165 170 175
Ala Cys Asp Ala Met Val His Arg Gly Trp Leu Ser Met Tyr Thr Ser
180 185 190
Ile Asp Pro Ala Ser Ser His Asn Gln Asp Ser Ala Arg His Gln Ala
195 200 205
Leu Ser Glu Val Arg Arg Leu Val Asp Ala Tyr Ser Asp Glu Glu Arg
210 215 220
Ser Ile Thr Val Val Gly His Ser Leu Gly Ala Ala Leu Ala Thr Leu
225 230 235 240
Asn Ala Phe Asp Ile Ala Ala Asn Gly Tyr Asn Val Ala Thr Gly Ala
245 250 255
Ala Ala Cys Pro Val Thr Ala Phe Ala Phe Ala Ser Pro Arg Val Gly
260 265 270
Gly Gly Gly Phe Lys Lys Arg Phe Asp Ala Val Pro Gly Leu Arg Leu
275 280 285
Leu Arg Val Arg Asn Ala Arg Asp Val Val Pro Lys Tyr Pro Ile Val
290 295 300
Phe Tyr His Asp Val Gly Ala Glu Leu Ala Ile Asp Thr Gly Glu Ser
305 310 315 320
Pro Tyr Leu Arg Ser Pro Gly Arg Glu His Thr Trp His Asn Leu Glu
325 330 335
Val Tyr Leu His Gly Val Ala Gly Thr Arg Gly Ala Arg Gly Gly Phe
340 345 350
Glu Leu Ala Val Ala Arg Asp Val Ala Leu Val Asn Lys Leu Tyr Asp
355 360 365
Val Leu Trp Asp Asp Tyr Gly Val Pro Pro Gly Trp Trp Val Pro Leu
370 375 380
Asn Lys Gly Met Val Glu Gly Ala Asp Gly Arg Trp Ser Leu Met Asp
385 390 395 400
Cys Glu Glu Asp Glu Glu Asp Glu
405
<210> 89
<211> 1260
<212> DNA
<213> Arabidopsis thaliana
<400> 89
atgaagagga agaagaaaga ggaggaggag gagaagttga tagtgactag agaattcgcg 60
aagagatggc gagatctgag cggtcaaaac cattggaaag ggatgttaca gccgttggat 120
caagatctcc gggaatatat catacattat ggtgagatgg ctcaggctgg ttacgatact 180
ttcaatatca acaccgaatc tcagttcgcc ggtgctagca tctactctag aaaagacttc 240
ttcgccaagg ttggtttaga gatagcacac ccgtacacaa agtacaaagt gacgaagttt 300
atatacgcga catccgatat ccacgtgcct gagtcattct tattgttccc aatttcacgt 360
gagggatggt caaaggaatc aaattggatg ggttacgtgg cggtaacaga cgaccaaggc 420
acggcacttc ttggtcggag agatatcgtg gtttcttgga gaggctcagt gcaaccactt 480
gaatgggtcg aagacttcga gtttggttta gtcaacgcca taaaaatctt cggtgaaaga 540
aacgatcaag tacaaattca tcaaggttgg tattcaatct acatgtctca agatgaacga 600
tcacctttta ctaagaccaa tgctcgtgac caggtattgc gagaggttgg aagattgttg 660
gagaagtata aggacgaaga agttagtata actatatgtg gtcatagtct tggagctgca 720
ctcgcgacgc ttagtgccac tgatattgtg gctaatggtt ataaccgacc aaagagccgt 780
cctgacaagt cttgtcctgt tactgccttt gtctttgcta gtcctcgtgt tggggattca 840
gactttagga aactcttttc cggattggaa gatatccgag tgttacggac aaggaatcta 900
ccggacgtaa ttccgatcta tccaccgata ggttactccg aggtaggaga tgagtttcca 960
atagacacaa gaaagtcacc atacatgaaa tctcctggaa acctcgcaac ctttcactgc 1020
ttagagggtt acttgcatgg cgttgccggg actcaaggaa cgaacaaagc tgacttattc 1080
agactcgatg tggaaagagc catcggattg gtcaataaat cagtggatgg gttaaaagac 1140
gagtgtatgg tcccagggaa atggagggtt cttaaaaaca aaggtatggc ccaacaagac 1200
gatggatctt gggagttagt ggaccatgag attgatgata atgaagattt ggatttctga 1260
<210> 90
<211> 419
<212> PRT
<213> Arabidopsis thaliana
<400> 90
Met Lys Arg Lys Lys Lys Glu Glu Glu Glu Glu Lys Leu Ile Val Thr
1 5 10 15
Arg Glu Phe Ala Lys Arg Trp Arg Asp Leu Ser Gly Gln Asn His Trp
20 25 30
Lys Gly Met Leu Gln Pro Leu Asp Gln Asp Leu Arg Glu Tyr Ile Ile
35 40 45
His Tyr Gly Glu Met Ala Gln Ala Gly Tyr Asp Thr Phe Asn Ile Asn
50 55 60
Thr Glu Ser Gln Phe Ala Gly Ala Ser Ile Tyr Ser Arg Lys Asp Phe
65 70 75 80
Phe Ala Lys Val Gly Leu Glu Ile Ala His Pro Tyr Thr Lys Tyr Lys
85 90 95
Val Thr Lys Phe Ile Tyr Ala Thr Ser Asp Ile His Val Pro Glu Ser
100 105 110
Phe Leu Leu Phe Pro Ile Ser Arg Glu Gly Trp Ser Lys Glu Ser Asn
115 120 125
Trp Met Gly Tyr Val Ala Val Thr Asp Asp Gln Gly Thr Ala Leu Leu
130 135 140
Gly Arg Arg Asp Ile Val Val Ser Trp Arg Gly Ser Val Gln Pro Leu
145 150 155 160
Glu Trp Val Glu Asp Phe Glu Phe Gly Leu Val Asn Ala Ile Lys Ile
165 170 175
Phe Gly Glu Arg Asn Asp Gln Val Gln Ile His Gln Gly Trp Tyr Ser
180 185 190
Ile Tyr Met Ser Gln Asp Glu Arg Ser Pro Phe Thr Lys Thr Asn Ala
195 200 205
Arg Asp Gln Val Leu Arg Glu Val Gly Arg Leu Leu Glu Lys Tyr Lys
210 215 220
Asp Glu Glu Val Ser Ile Thr Ile Cys Gly His Ser Leu Gly Ala Ala
225 230 235 240
Leu Ala Thr Leu Ser Ala Thr Asp Ile Val Ala Asn Gly Tyr Asn Arg
245 250 255
Pro Lys Ser Arg Pro Asp Lys Ser Cys Pro Val Thr Ala Phe Val Phe
260 265 270
Ala Ser Pro Arg Val Gly Asp Ser Asp Phe Arg Lys Leu Phe Ser Gly
275 280 285
Leu Glu Asp Ile Arg Val Leu Arg Thr Arg Asn Leu Pro Asp Val Ile
290 295 300
Pro Ile Tyr Pro Pro Ile Gly Tyr Ser Glu Val Gly Asp Glu Phe Pro
305 310 315 320
Ile Asp Thr Arg Lys Ser Pro Tyr Met Lys Ser Pro Gly Asn Leu Ala
325 330 335
Thr Phe His Cys Leu Glu Gly Tyr Leu His Gly Val Ala Gly Thr Gln
340 345 350
Gly Thr Asn Lys Ala Asp Leu Phe Arg Leu Asp Val Glu Arg Ala Ile
355 360 365
Gly Leu Val Asn Lys Ser Val Asp Gly Leu Lys Asp Glu Cys Met Val
370 375 380
Pro Gly Lys Trp Arg Val Leu Lys Asn Lys Gly Met Ala Gln Gln Asp
385 390 395 400
Asp Gly Ser Trp Glu Leu Val Asp His Glu Ile Asp Asp Asn Glu Asp
405 410 415
Leu Asp Phe
<210> 91
<211> 1206
<212> DNA
<213> Glycine max
<400> 91
atggaaaaga agatcatgaa tagcagcata gcgaaaaaat ggcgacaact cagtgggcaa 60
gatcattgga agggtctgat agaccctctg gacattgatc ttcgccgcta catcattcac 120
tacggcgaaa tggctcaagc ggcatacgat gctttcaaca cggagaaagc atcaaagtac 180
gctggaagca gcagatacgc caaaaagagt ttcttctcca aagttggttt ggtgaatggc 240
aaccccttca cgtattccgt gacaaagttt ctgtatgcaa cttcggaaat tgacgtccct 300
gatgcgttca tcataaagtc gttttctagg gaggcttgga gcagggaatc gaattggata 360
gggtatgttg cggtggccac agatgaaggg aaagctgcgt tgggtagaag ggacattgtg 420
attgcgtgga gaggcacggt gcagacattg gagtgggtta atgatcttca gtttctgttg 480
gttcctgctc ctaaagtgtt tggtaagaac acagatccta aagtgcacca gggctggtac 540
tccatttaca cctctgagga ccctcgttct ccctttaata aaaccagtgc tagaacccag 600
gtcctaagtg aggtgagaag actggtggag ctatataaga atgaagaaat aagcataaca 660
ataaccggtc acagtttagg tgctgcaatt gcaaccctta atgcagtgga cattgttaca 720
aatgggtaca acaagccaag tgatccatct ctcaaggctt ctccagttac tgccattgtg 780
tttgccagcc caagagttgg tgacataaac tttcagaagg ttttttctgg ctacaaagat 840
ttgacaacca tacgcatccg aaacgagtta gatattgttc caaactaccc tcttgttggg 900
tattcggatg tgggtgagga gttgaagatc gatacccgaa aatcgatgta cttgaagagt 960
cctgggaatc catctagttg gcataatttg gaggcttatt tgcatggagt ggcaggaaca 1020
caaaggagca aaggagggtt caaattagag gttcatcgcg atattgcact tgtgaataag 1080
accttggatg ctctaaaaga tgaattcctt gtgccagtgt catggaggac tgaaaagaat 1140
aagggtatgg ttcaacaaaa tgatggttcg tggaagctga tggatcatga agatgatgac 1200
ttttga 1206
<210> 92
<211> 401
<212> PRT
<213> Glycine max
<400> 92
Met Glu Lys Lys Ile Met Asn Ser Ser Ile Ala Lys Lys Trp Arg Gln
1 5 10 15
Leu Ser Gly Gln Asp His Trp Lys Gly Leu Ile Asp Pro Leu Asp Ile
20 25 30
Asp Leu Arg Arg Tyr Ile Ile His Tyr Gly Glu Met Ala Gln Ala Ala
35 40 45
Tyr Asp Ala Phe Asn Thr Glu Lys Ala Ser Lys Tyr Ala Gly Ser Ser
50 55 60
Arg Tyr Ala Lys Lys Ser Phe Phe Ser Lys Val Gly Leu Val Asn Gly
65 70 75 80
Asn Pro Phe Thr Tyr Ser Val Thr Lys Phe Leu Tyr Ala Thr Ser Glu
85 90 95
Ile Asp Val Pro Asp Ala Phe Ile Ile Lys Ser Phe Ser Arg Glu Ala
100 105 110
Trp Ser Arg Glu Ser Asn Trp Ile Gly Tyr Val Ala Val Ala Thr Asp
115 120 125
Glu Gly Lys Ala Ala Leu Gly Arg Arg Asp Ile Val Ile Ala Trp Arg
130 135 140
Gly Thr Val Gln Thr Leu Glu Trp Val Asn Asp Leu Gln Phe Leu Leu
145 150 155 160
Val Pro Ala Pro Lys Val Phe Gly Lys Asn Thr Asp Pro Lys Val His
165 170 175
Gln Gly Trp Tyr Ser Ile Tyr Thr Ser Glu Asp Pro Arg Ser Pro Phe
180 185 190
Asn Lys Thr Ser Ala Arg Thr Gln Val Leu Ser Glu Val Arg Arg Leu
195 200 205
Val Glu Leu Tyr Lys Asn Glu Glu Ile Ser Ile Thr Ile Thr Gly His
210 215 220
Ser Leu Gly Ala Ala Ile Ala Thr Leu Asn Ala Val Asp Ile Val Thr
225 230 235 240
Asn Gly Tyr Asn Lys Pro Ser Asp Pro Ser Leu Lys Ala Ser Pro Val
245 250 255
Thr Ala Ile Val Phe Ala Ser Pro Arg Val Gly Asp Ile Asn Phe Gln
260 265 270
Lys Val Phe Ser Gly Tyr Lys Asp Leu Thr Thr Ile Arg Ile Arg Asn
275 280 285
Glu Leu Asp Ile Val Pro Asn Tyr Pro Leu Val Gly Tyr Ser Asp Val
290 295 300
Gly Glu Glu Leu Lys Ile Asp Thr Arg Lys Ser Met Tyr Leu Lys Ser
305 310 315 320
Pro Gly Asn Pro Ser Ser Trp His Asn Leu Glu Ala Tyr Leu His Gly
325 330 335
Val Ala Gly Thr Gln Arg Ser Lys Gly Gly Phe Lys Leu Glu Val His
340 345 350
Arg Asp Ile Ala Leu Val Asn Lys Thr Leu Asp Ala Leu Lys Asp Glu
355 360 365
Phe Leu Val Pro Val Ser Trp Arg Thr Glu Lys Asn Lys Gly Met Val
370 375 380
Gln Gln Asn Asp Gly Ser Trp Lys Leu Met Asp His Glu Asp Asp Asp
385 390 395 400
Phe
<210> 93
<211> 978
<212> DNA
<213> Oryza sativa
<400> 93
atgccgatgc cgttatatgg ggtggtcaac tcgccgtgcc gcagccactg cagggccgcg 60
gcaacgttgt tggtctcggt gtccctgctc tgctcttgct tcgccatcgc catgccgagc 120
gtcgtcgtca cggtcgacca gtccggcaag ggcgaccacc ggaggatcca ggacgcgatc 180
gatgccgccc cggcaaacga ctcctcccgc accgtcatcc ggatcaagcc cggggtttac 240
agggagaagg tcgtggtgga caagccgtac gtgacgctga ccggcacgag cgcgacctcg 300
acggtgatcg cctggaacga gtcgtgggtc tccgacgagt cccccaccgt gtccgtgctg 360
gcctccgact tcgtcgccaa gcgcctgacg tttcagaaca cgttcgggga cagcgcgccg 420
gcggtggcgg tgagggtcgc cggagacagg gcggcgttct acgggtgcag gttcgtgtcg 480
ttccaggaca cgctcctcga cgagacgggg cggcactact accgcggctg ctacgtgcag 540
ggcgccaccg acttcatatt cggaaacggc cgggctctgt ttgacaaatg ccacctgcac 600
tccacgtcgc ccgacggcgc cggcggggcg ttcacggcgc agcagcggtc gtcggaatcg 660
gaggagacgg ggtacagctt cgtggggtgc aagctgaccg gcctcggcgc cggcacctcc 720
atcctgggcc ggccgtgggg gccatactcc cgcgtcgtct tcgcgctcac ctacatgtcc 780
tccaccgtca ggccccaagg ctgggacgac tggggcgacc cctccaatca gcggacggcg 840
ttctatgggc agtaccagtg ctacggcgac ggatcgaaga ccgacggcag ggttgcctgg 900
tctcacgacc tgacgcaggc cgaggcggcg ccgttcatca ccaaggcttg ggttgatggg 960
cagcaatggc ttcggtag 978
<210> 94
<211> 325
<212> PRT
<213> Oryza sativa
<400> 94
Met Pro Met Pro Leu Tyr Gly Val Val Asn Ser Pro Cys Arg Ser His
1 5 10 15
Cys Arg Ala Ala Ala Thr Leu Leu Val Ser Val Ser Leu Leu Cys Ser
20 25 30
Cys Phe Ala Ile Ala Met Pro Ser Val Val Val Thr Val Asp Gln Ser
35 40 45
Gly Lys Gly Asp His Arg Arg Ile Gln Asp Ala Ile Asp Ala Ala Pro
50 55 60
Ala Asn Asp Ser Ser Arg Thr Val Ile Arg Ile Lys Pro Gly Val Tyr
65 70 75 80
Arg Glu Lys Val Val Val Asp Lys Pro Tyr Val Thr Leu Thr Gly Thr
85 90 95
Ser Ala Thr Ser Thr Val Ile Ala Trp Asn Glu Ser Trp Val Ser Asp
100 105 110
Glu Ser Pro Thr Val Ser Val Leu Ala Ser Asp Phe Val Ala Lys Arg
115 120 125
Leu Thr Phe Gln Asn Thr Phe Gly Asp Ser Ala Pro Ala Val Ala Val
130 135 140
Arg Val Ala Gly Asp Arg Ala Ala Phe Tyr Gly Cys Arg Phe Val Ser
145 150 155 160
Phe Gln Asp Thr Leu Leu Asp Glu Thr Gly Arg His Tyr Tyr Arg Gly
165 170 175
Cys Tyr Val Gln Gly Ala Thr Asp Phe Ile Phe Gly Asn Gly Arg Ala
180 185 190
Leu Phe Asp Lys Cys His Leu His Ser Thr Ser Pro Asp Gly Ala Gly
195 200 205
Gly Ala Phe Thr Ala Gln Gln Arg Ser Ser Glu Ser Glu Glu Thr Gly
210 215 220
Tyr Ser Phe Val Gly Cys Lys Leu Thr Gly Leu Gly Ala Gly Thr Ser
225 230 235 240
Ile Leu Gly Arg Pro Trp Gly Pro Tyr Ser Arg Val Val Phe Ala Leu
245 250 255
Thr Tyr Met Ser Ser Thr Val Arg Pro Gln Gly Trp Asp Asp Trp Gly
260 265 270
Asp Pro Ser Asn Gln Arg Thr Ala Phe Tyr Gly Gln Tyr Gln Cys Tyr
275 280 285
Gly Asp Gly Ser Lys Thr Asp Gly Arg Val Ala Trp Ser His Asp Leu
290 295 300
Thr Gln Ala Glu Ala Ala Pro Phe Ile Thr Lys Ala Trp Val Asp Gly
305 310 315 320
Gln Gln Trp Leu Arg
325
<210> 95
<211> 786
<212> DNA
<213> Zea mays
<400> 95
atgcatcgtg caccaagtga gaaggtgatc gtaccgcaga acaagagctt catcctgctg 60
gaaggcgagg ggtggcagca gacgtcgatc gagtgggcgg accacgccgg cggggactcg 120
acaaccgccg cctccccgac gttcgccgcg tactcggacg acttcatggc ccgagacatc 180
accttcaaga acacgtacaa cggggacggg aggatcgcgc cggcggtggc ggcgctggcg 240
gcgggagatc ggtcgtcctt ctaccggtgc ggcttcgtga gcgtccagga cacgctgagc 300
gacctcgagg ggaggcacta ctacgagggc tgctacatcg agggcgccat ggacttcatc 360
ttcggcaacg gtcagtccat cttccagggc tgcgagatat ggacggcccg gacgccggtg 420
tggcccggct tcatcacggc gcaggggcgg atgagcgagg cggactccag cgggttcgtc 480
ttcaagggat gcacggtgag gggggtgacg ccggcgtacc tgggccgcgc gtggcgccgc 540
tacgccaggg tcatcttctt ccagacggac atgtccggcg tcgtcgttag ccaggggtgg 600
gacgcgtgga gctacaaggg cacagagggc acgctgacca tggtggaaga agggtgcacg 660
gggcaggggt ccaaccggac gggccgggtg ccatggacca aggacctcag cggcgacgac 720
ctcgccaagt tcgtcgacct ctcctacgtc tctgccgacg gctggctcga cgcacagcca 780
cgctag 786
<210> 96
<211> 261
<212> PRT
<213> Zea mays
<400> 96
Met His Arg Ala Pro Ser Glu Lys Val Ile Val Pro Gln Asn Lys Ser
1 5 10 15
Phe Ile Leu Leu Glu Gly Glu Gly Trp Gln Gln Thr Ser Ile Glu Trp
20 25 30
Ala Asp His Ala Gly Gly Asp Ser Thr Thr Ala Ala Ser Pro Thr Phe
35 40 45
Ala Ala Tyr Ser Asp Asp Phe Met Ala Arg Asp Ile Thr Phe Lys Asn
50 55 60
Thr Tyr Asn Gly Asp Gly Arg Ile Ala Pro Ala Val Ala Ala Leu Ala
65 70 75 80
Ala Gly Asp Arg Ser Ser Phe Tyr Arg Cys Gly Phe Val Ser Val Gln
85 90 95
Asp Thr Leu Ser Asp Leu Glu Gly Arg His Tyr Tyr Glu Gly Cys Tyr
100 105 110
Ile Glu Gly Ala Met Asp Phe Ile Phe Gly Asn Gly Gln Ser Ile Phe
115 120 125
Gln Gly Cys Glu Ile Trp Thr Ala Arg Thr Pro Val Trp Pro Gly Phe
130 135 140
Ile Thr Ala Gln Gly Arg Met Ser Glu Ala Asp Ser Ser Gly Phe Val
145 150 155 160
Phe Lys Gly Cys Thr Val Arg Gly Val Thr Pro Ala Tyr Leu Gly Arg
165 170 175
Ala Trp Arg Arg Tyr Ala Arg Val Ile Phe Phe Gln Thr Asp Met Ser
180 185 190
Gly Val Val Val Ser Gln Gly Trp Asp Ala Trp Ser Tyr Lys Gly Thr
195 200 205
Glu Gly Thr Leu Thr Met Val Glu Glu Gly Cys Thr Gly Gln Gly Ser
210 215 220
Asn Arg Thr Gly Arg Val Pro Trp Thr Lys Asp Leu Ser Gly Asp Asp
225 230 235 240
Leu Ala Lys Phe Val Asp Leu Ser Tyr Val Ser Ala Asp Gly Trp Leu
245 250 255
Asp Ala Gln Pro Arg
260
<210> 97
<211> 1017
<212> DNA
<213> Sorghum bicolor
<400> 97
atgacgaggc tccggctagg acctccgttg ttcaaaatgg ccctagccgt cttagccgcc 60
gtgctcgctt ccatggcgcc ccgaggtcgc tgcgacgacg cggcgaggtc cgtggtggcc 120
agaagcatct tcgtcaaccg gaagggcggc gccgacttca cgtcggtcca ggacgccgtc 180
gactctgttc cgctcggcaa cgaccagtgg atccgagttc acgtcgccgc cggcgtgtac 240
aatgagaagg tgatgatacc gcagaacaag agcttcatcc tgctggaagg cgaggggtgg 300
cagcagacgt cgatcgagtg ggcggaccac gccggcgggg actcgagcac cgccgccacc 360
ccaacgttcg cggcgtactc agccgacttc atggcccgcg acatcgcctt caagaacacg 420
tacaacggtg ccggcgggac gacgacgatc gcgccggcgg tggcggcgct ggtggcgggc 480
gaccggtcgt ccttctaccg gtgcggcttc gtgagcgtgc aggacacgct gagcgacatc 540
caagggaggc actactacga gggctgccac atccagggcg ccatggactt catcttcggc 600
aacggccagt ccatcttcca ggggtgcgag atatggacgg cgcggacgcc cgtgtggcca 660
ggcttcatca cggcgcaggg gagggtgagc gaggcggaca cgagcggctt cgtcttcaag 720
ggctgcacgg tgaggggcgt cacgccggcg tacctgggac gcgcgtggcg acgctacgcc 780
agggtcatct tctaccagac ggacatgtcc ggcgtcgtca gccaggggtg ggacgcgtgg 840
ggctacaagg gcacagaggg cacgctgact atggtggagg aagggtgcac ggggcagggg 900
tccaacagga cgggccgggt gccatggact aaggacctga gcggcgccga actcgccaag 960
ttcgtcgacc tctcctacgt ctctgccgac ggctggctcg acgcgcagcc acgctag 1017
<210> 98
<211> 338
<212> PRT
<213> Sorghum bicolor
<400> 98
Met Thr Arg Leu Arg Leu Gly Pro Pro Leu Phe Lys Met Ala Leu Ala
1 5 10 15
Val Leu Ala Ala Val Leu Ala Ser Met Ala Pro Arg Gly Arg Cys Asp
20 25 30
Asp Ala Ala Arg Ser Val Val Ala Arg Ser Ile Phe Val Asn Arg Lys
35 40 45
Gly Gly Ala Asp Phe Thr Ser Val Gln Asp Ala Val Asp Ser Val Pro
50 55 60
Leu Gly Asn Asp Gln Trp Ile Arg Val His Val Ala Ala Gly Val Tyr
65 70 75 80
Asn Glu Lys Val Met Ile Pro Gln Asn Lys Ser Phe Ile Leu Leu Glu
85 90 95
Gly Glu Gly Trp Gln Gln Thr Ser Ile Glu Trp Ala Asp His Ala Gly
100 105 110
Gly Asp Ser Ser Thr Ala Ala Thr Pro Thr Phe Ala Ala Tyr Ser Ala
115 120 125
Asp Phe Met Ala Arg Asp Ile Ala Phe Lys Asn Thr Tyr Asn Gly Ala
130 135 140
Gly Gly Thr Thr Thr Ile Ala Pro Ala Val Ala Ala Leu Val Ala Gly
145 150 155 160
Asp Arg Ser Ser Phe Tyr Arg Cys Gly Phe Val Ser Val Gln Asp Thr
165 170 175
Leu Ser Asp Ile Gln Gly Arg His Tyr Tyr Glu Gly Cys His Ile Gln
180 185 190
Gly Ala Met Asp Phe Ile Phe Gly Asn Gly Gln Ser Ile Phe Gln Gly
195 200 205
Cys Glu Ile Trp Thr Ala Arg Thr Pro Val Trp Pro Gly Phe Ile Thr
210 215 220
Ala Gln Gly Arg Val Ser Glu Ala Asp Thr Ser Gly Phe Val Phe Lys
225 230 235 240
Gly Cys Thr Val Arg Gly Val Thr Pro Ala Tyr Leu Gly Arg Ala Trp
245 250 255
Arg Arg Tyr Ala Arg Val Ile Phe Tyr Gln Thr Asp Met Ser Gly Val
260 265 270
Val Ser Gln Gly Trp Asp Ala Trp Gly Tyr Lys Gly Thr Glu Gly Thr
275 280 285
Leu Thr Met Val Glu Glu Gly Cys Thr Gly Gln Gly Ser Asn Arg Thr
290 295 300
Gly Arg Val Pro Trp Thr Lys Asp Leu Ser Gly Ala Glu Leu Ala Lys
305 310 315 320
Phe Val Asp Leu Ser Tyr Val Ser Ala Asp Gly Trp Leu Asp Ala Gln
325 330 335
Pro Arg
<210> 99
<211> 993
<212> DNA
<213> Arabidopsis thaliana
<400> 99
atgggaactc atcgaattat ccttggtctc gccgctcttt gttgtttttg tctacctcat 60
ctcattgaag ctaaaccatt cgaagtgatc gtcgatcaat cgggccatgg aaatttcacg 120
accatacaaa aagctattga ttcggtccca atcaacaata ctcattggtt cttcatcaac 180
gttaaagccg gcctttacag agagaaaata acgataccgc aaaaaaaacc tttcatagta 240
atcgtgggag ctgggaaacg gtcaacgaga gtagaatggg acgaccatgc ctcgcttgca 300
caaagcccta cctttgctac tctagctgat aacaccgtcg ttaaaaagat cactttcgcc 360
aattcctaca acttccctag caatgggaaa ataaacaaga accctagagt tccggccgtg 420
gcagcattta tcggcggtga taagtcggct ttttactcgg taggatttgc tggaattcaa 480
gataccttgt gggactcgga tggccgacac tacttccata gatgcaccat ccaaggcgcg 540
gttgatttca tcttagggag cggccaatct atttaccaga gctgtgtgat acaagtgcta 600
ggtgggcagc taggaccggg ggtaacgggt tacataacgg ctcaaggacg gaccaacgca 660
aacgacgcga atggattcgt attcatcaac tgcctcgttc atggattcgg caaggcttac 720
ttgggcagag cctggcgccc atactctcga gtgatatttt acaactcgaa cctgacggat 780
gtggttgatc ctctaggatg gtgggaatgg aactaccagg gttacgaaaa gcagctgacc 840
tatgcagaac atggatgctt cggaagtgga tcaaatacat caagacgtgc caaatgggtt 900
aagaagctga gtgcatctgc cgtccaacat ttggctgatc tcagcttcat taatcgtggt 960
ggatgggttg aagatttacc catccgtgtt tga 993
<210> 100
<211> 330
<212> PRT
<213> Arabidopsis thaliana
<400> 100
Met Gly Thr His Arg Ile Ile Leu Gly Leu Ala Ala Leu Cys Cys Phe
1 5 10 15
Cys Leu Pro His Leu Ile Glu Ala Lys Pro Phe Glu Val Ile Val Asp
20 25 30
Gln Ser Gly His Gly Asn Phe Thr Thr Ile Gln Lys Ala Ile Asp Ser
35 40 45
Val Pro Ile Asn Asn Thr His Trp Phe Phe Ile Asn Val Lys Ala Gly
50 55 60
Leu Tyr Arg Glu Lys Ile Thr Ile Pro Gln Lys Lys Pro Phe Ile Val
65 70 75 80
Ile Val Gly Ala Gly Lys Arg Ser Thr Arg Val Glu Trp Asp Asp His
85 90 95
Ala Ser Leu Ala Gln Ser Pro Thr Phe Ala Thr Leu Ala Asp Asn Thr
100 105 110
Val Val Lys Lys Ile Thr Phe Ala Asn Ser Tyr Asn Phe Pro Ser Asn
115 120 125
Gly Lys Ile Asn Lys Asn Pro Arg Val Pro Ala Val Ala Ala Phe Ile
130 135 140
Gly Gly Asp Lys Ser Ala Phe Tyr Ser Val Gly Phe Ala Gly Ile Gln
145 150 155 160
Asp Thr Leu Trp Asp Ser Asp Gly Arg His Tyr Phe His Arg Cys Thr
165 170 175
Ile Gln Gly Ala Val Asp Phe Ile Leu Gly Ser Gly Gln Ser Ile Tyr
180 185 190
Gln Ser Cys Val Ile Gln Val Leu Gly Gly Gln Leu Gly Pro Gly Val
195 200 205
Thr Gly Tyr Ile Thr Ala Gln Gly Arg Thr Asn Ala Asn Asp Ala Asn
210 215 220
Gly Phe Val Phe Ile Asn Cys Leu Val His Gly Phe Gly Lys Ala Tyr
225 230 235 240
Leu Gly Arg Ala Trp Arg Pro Tyr Ser Arg Val Ile Phe Tyr Asn Ser
245 250 255
Asn Leu Thr Asp Val Val Asp Pro Leu Gly Trp Trp Glu Trp Asn Tyr
260 265 270
Gln Gly Tyr Glu Lys Gln Leu Thr Tyr Ala Glu His Gly Cys Phe Gly
275 280 285
Ser Gly Ser Asn Thr Ser Arg Arg Ala Lys Trp Val Lys Lys Leu Ser
290 295 300
Ala Ser Ala Val Gln His Leu Ala Asp Leu Ser Phe Ile Asn Arg Gly
305 310 315 320
Gly Trp Val Glu Asp Leu Pro Ile Arg Val
325 330
<210> 101
<211> 960
<212> DNA
<213> Glycine max
<400> 101
atgtcaatca ccgccatcat tttgttcggc atcaccacca ccaccatgat tacaacaaac 60
aaactactaa gaaaacagtc cattattctt ccaggacaat tattgtggac cgattgggga 120
atggacactt ctctacgata caatctgcca ttgattcagt cgcttcctac aacaagaatt 180
ggggaaaagg tgaagatcac ttctgacaag cctttcatcg tactaaaagg agagggacaa 240
aagaacactt ttgttgagtg gcatgaccat gattcaagtg cagagagtcc tacgttcaca 300
accatggccg acaatgtcgt cgtcaagtcc attagcttta ggaatacata caataataac 360
agaaatgcta actcaatgga agctgcggta gctgcaatga tatttggcga caggtcgtac 420
ttctatgacg ttggcttctt tggtttgcaa gacactttgt gggatggaca aggaagacat 480
tacttcaagt cttgtacaat tcaaggtgct atggatttta tcttcggcac tggccaatct 540
ttatatgagg actgtaccat atccgctatt ggtgctaatc ttggtcctgg cattattggt 600
tttatcacgg cacaagggag aacaaatcca aacgatgcaa atgggtttgt ttttaagcat 660
tgcaatatcg ttggaaatgg tacaacttac ttgggaagac catggagagg ttatgctaga 720
gttcttttct atgatactaa aatctctaac attatccaac cattaggttg gcagccatgg 780
gatttcgctg ggcacgagga tcacataaca ttcgcagagt atggcaattc tggacctggt 840
tctgacactt ccaagcgagt aagttggtta aagaaattgg attcatcaac agttagcaag 900
ttggcaacta ctagcttcat tgacaccgaa ggttggctaa atacactgac gcagctctaa 960
<210> 102
<211> 319
<212> PRT
<213> Glycine max
<400> 102
Met Ser Ile Thr Ala Ile Ile Leu Phe Gly Ile Thr Thr Thr Thr Met
1 5 10 15
Ile Thr Thr Asn Lys Leu Leu Arg Lys Gln Ser Ile Ile Leu Pro Gly
20 25 30
Gln Leu Leu Trp Thr Asp Trp Gly Met Asp Thr Ser Leu Arg Tyr Asn
35 40 45
Leu Pro Leu Ile Gln Ser Leu Pro Thr Thr Arg Ile Gly Glu Lys Val
50 55 60
Lys Ile Thr Ser Asp Lys Pro Phe Ile Val Leu Lys Gly Glu Gly Gln
65 70 75 80
Lys Asn Thr Phe Val Glu Trp His Asp His Asp Ser Ser Ala Glu Ser
85 90 95
Pro Thr Phe Thr Thr Met Ala Asp Asn Val Val Val Lys Ser Ile Ser
100 105 110
Phe Arg Asn Thr Tyr Asn Asn Asn Arg Asn Ala Asn Ser Met Glu Ala
115 120 125
Ala Val Ala Ala Met Ile Phe Gly Asp Arg Ser Tyr Phe Tyr Asp Val
130 135 140
Gly Phe Phe Gly Leu Gln Asp Thr Leu Trp Asp Gly Gln Gly Arg His
145 150 155 160
Tyr Phe Lys Ser Cys Thr Ile Gln Gly Ala Met Asp Phe Ile Phe Gly
165 170 175
Thr Gly Gln Ser Leu Tyr Glu Asp Cys Thr Ile Ser Ala Ile Gly Ala
180 185 190
Asn Leu Gly Pro Gly Ile Ile Gly Phe Ile Thr Ala Gln Gly Arg Thr
195 200 205
Asn Pro Asn Asp Ala Asn Gly Phe Val Phe Lys His Cys Asn Ile Val
210 215 220
Gly Asn Gly Thr Thr Tyr Leu Gly Arg Pro Trp Arg Gly Tyr Ala Arg
225 230 235 240
Val Leu Phe Tyr Asp Thr Lys Ile Ser Asn Ile Ile Gln Pro Leu Gly
245 250 255
Trp Gln Pro Trp Asp Phe Ala Gly His Glu Asp His Ile Thr Phe Ala
260 265 270
Glu Tyr Gly Asn Ser Gly Pro Gly Ser Asp Thr Ser Lys Arg Val Ser
275 280 285
Trp Leu Lys Lys Leu Asp Ser Ser Thr Val Ser Lys Leu Ala Thr Thr
290 295 300
Ser Phe Ile Asp Thr Glu Gly Trp Leu Asn Thr Leu Thr Gln Leu
305 310 315
<210> 103
<211> 405
<212> DNA
<213> Oryza sativa
<400> 103
atgttaatga atctagatag atgtgaaacg gagggagtac atagcatcat ctcgacggtc 60
tgcgagcttg agcttgagat gtggtgtttc cccatctcat gtgaatcatt tgagccttcc 120
ggatcccaac tcctgaacca tccaaggcgt atccgtctcc atcatcaggc gttgctgttg 180
tttagacaag ctgatctacc gtctctcctt tcaagttaca tagttcaagt cgtcattcat 240
tacttcgaca aacagtctgc cgagttcatc aactcagctg ctggggccat caccgtctcc 300
tacatcatgc agctactgac tggactactt gggagggagt tttcagcagt aatatatgca 360
acacaaagta tatctaatat actttccatc agtggggaag agtag 405
<210> 104
<211> 134
<212> PRT
<213> Oryza sativa
<400> 104
Met Leu Met Asn Leu Asp Arg Cys Glu Thr Glu Gly Val His Ser Ile
1 5 10 15
Ile Ser Thr Val Cys Glu Leu Glu Leu Glu Met Trp Cys Phe Pro Ile
20 25 30
Ser Cys Glu Ser Phe Glu Pro Ser Gly Ser Gln Leu Leu Asn His Pro
35 40 45
Arg Arg Ile Arg Leu His His Gln Ala Leu Leu Leu Phe Arg Gln Ala
50 55 60
Asp Leu Pro Ser Leu Leu Ser Ser Tyr Ile Val Gln Val Val Ile His
65 70 75 80
Tyr Phe Asp Lys Gln Ser Ala Glu Phe Ile Asn Ser Ala Ala Gly Ala
85 90 95
Ile Thr Val Ser Tyr Ile Met Gln Leu Leu Thr Gly Leu Leu Gly Arg
100 105 110
Glu Phe Ser Ala Val Ile Tyr Ala Thr Gln Ser Ile Ser Asn Ile Leu
115 120 125
Ser Ile Ser Gly Glu Glu
130
<210> 105
<211> 2289
<212> DNA
<213> Oryza sativa
<400> 105
atgtggaggc tgaaggtggc ccagggcggc ggcgctttgt tgcggtcgac gaacggcttc 60
gccgggcgag cggtgtggga gttcgacccc gaccacggca cgccggagga ccgagccaac 120
gtcgagaggg tgcgccgcga cttcaccgac caccgcctcc gccgcccgga gtccgccgat 180
ctcctcatgc gcatgcagtt tgcaagagaa aacaatcacc aacgccgtgg tgatcgtatt 240
ccaccggccg tcaataagct tggcgagaag gagcaggtga ctgaagaaac tgtgatggct 300
tccttgaggc gagctctcga tgagttctcc tctctgcaag cagatgatgg ccactggcct 360
ggcgacctca gtggtgctat attcatcatg cctgtcttga tattttcttt gtatgccact 420
ggatcactgg acactgtcat atcatcagaa caccggaggg agatatgtcg ctacatctat 480
aaccatcaga acgaggacgg gggatggggc atgctaatct tgggatcaag cacgatgttt 540
gccacatgct taaactatgt caccctgaga cttatcggcg aggagccaag caatgagcaa 600
ttagctagag gacacgcctg gatcatatca catggtggcg ccacccttgt tccgcaatgg 660
ggaaagatat gtctgtcgat aattggagtg tatgagtggt caggaaacaa ccccatcttc 720
cccgagttat ggcttgctcc acaattcctt ccgtttcatc caggcaaatt ctggggcccg 780
acccgcgtgg tgtacgtgcc gatggcctat ctgtacggca agaagttcgt ggggcccatc 840
acgccaacca tactggcgct gcgagaggag atatacacgg acccctacca caccattgac 900
tgggcccaag cctgcaacgc atgctccaag gaggatttgg tctgcccgcg cacgctgctg 960
cagaatgtgg tgtggacttc gctctacaag tgggtggagc cggtgctagg cagcaggccg 1020
atgaacaagc tgagggagag agctttggat agactcatgg agcatatcca ttacgaagat 1080
gaaaactcac agtacctctg cctatgtcct gtcaacaagg ctcttaacat ggtctgctgc 1140
tgggtggaag atccaaattc agattcattc aagcggcatc ttgcaaggat acctgatttc 1200
ctgtggcttt cagaagatgg catgaaggca cagatatatg atggttgcca gagctgggaa 1260
acagcattta taattcaagc gttttgtgct actgatcttg taaatgagta tggttcaact 1320
gttaggagag cccacgagtt catgaaaaat tcacagatta tgaggaatca tcctggtgac 1380
caaagttact ggcatcgcca tagatcaaag ggttcatgga ctctttcgtc agcagacaat 1440
ggatgggctg tgtctgacac tacagcagaa gcgctgaagg ctgtactgtt actggaaaag 1500
atctctagca acgtggtcgg ggatccaatt gaaatagaaa ggctgcatga tgctgttgat 1560
tgcctcctat ctttcgtgaa caaagatggt accctttcta catatgaatg taaaagaact 1620
tatacttgga tagaggttct cagtccttgt gagagttttc caaacatagt ggttgattat 1680
ccatttccag aatgcacctc ctctgtgctt caagctctgg tgctgttcaa acaactacac 1740
cctagttacc gtattaaaga gatagaaaaa tgtgtcagaa acgcagcgat gtttattgag 1800
agcacacaag gcgaagatgg ttcatggcta ggcacttggg gtgtgtgttt cacctatggg 1860
gcctttcttt cagtaaaagg gttaattgct gctggaagaa catatgagaa tagttcttcc 1920
atcaggaaag catgcgactt tatattatca aagcagctcg atactggtgg gtggggagaa 1980
agttatgtct ctaatattac taaggtttac gtaaacatta aagatgatca agctcatgca 2040
gtgaatactg cctgggcaat gttagcttta atttgtgcag gacagatgga gcgggatcca 2100
gcaccactgc atcgtgctgc aaaagaatta atcaatatgc agttggaaac aggagagttc 2160
ccccaacaag aacatgttgg agccttcaat gcatgcctct tctttaatta ccccaactac 2220
cgcaacttat tccctatctg ggctcttgga gagtactgtc gtcatcttca ctccacgagg 2280
cgtgcatga 2289
<210> 106
<211> 762
<212> PRT
<213> Oryza sativa
<400> 106
Met Trp Arg Leu Lys Val Ala Gln Gly Gly Gly Ala Leu Leu Arg Ser
1 5 10 15
Thr Asn Gly Phe Ala Gly Arg Ala Val Trp Glu Phe Asp Pro Asp His
20 25 30
Gly Thr Pro Glu Asp Arg Ala Asn Val Glu Arg Val Arg Arg Asp Phe
35 40 45
Thr Asp His Arg Leu Arg Arg Pro Glu Ser Ala Asp Leu Leu Met Arg
50 55 60
Met Gln Phe Ala Arg Glu Asn Asn His Gln Arg Arg Gly Asp Arg Ile
65 70 75 80
Pro Pro Ala Val Asn Lys Leu Gly Glu Lys Glu Gln Val Thr Glu Glu
85 90 95
Thr Val Met Ala Ser Leu Arg Arg Ala Leu Asp Glu Phe Ser Ser Leu
100 105 110
Gln Ala Asp Asp Gly His Trp Pro Gly Asp Leu Ser Gly Ala Ile Phe
115 120 125
Ile Met Pro Val Leu Ile Phe Ser Leu Tyr Ala Thr Gly Ser Leu Asp
130 135 140
Thr Val Ile Ser Ser Glu His Arg Arg Glu Ile Cys Arg Tyr Ile Tyr
145 150 155 160
Asn His Gln Asn Glu Asp Gly Gly Trp Gly Met Leu Ile Leu Gly Ser
165 170 175
Ser Thr Met Phe Ala Thr Cys Leu Asn Tyr Val Thr Leu Arg Leu Ile
180 185 190
Gly Glu Glu Pro Ser Asn Glu Gln Leu Ala Arg Gly His Ala Trp Ile
195 200 205
Ile Ser His Gly Gly Ala Thr Leu Val Pro Gln Trp Gly Lys Ile Cys
210 215 220
Leu Ser Ile Ile Gly Val Tyr Glu Trp Ser Gly Asn Asn Pro Ile Phe
225 230 235 240
Pro Glu Leu Trp Leu Ala Pro Gln Phe Leu Pro Phe His Pro Gly Lys
245 250 255
Phe Trp Gly Pro Thr Arg Val Val Tyr Val Pro Met Ala Tyr Leu Tyr
260 265 270
Gly Lys Lys Phe Val Gly Pro Ile Thr Pro Thr Ile Leu Ala Leu Arg
275 280 285
Glu Glu Ile Tyr Thr Asp Pro Tyr His Thr Ile Asp Trp Ala Gln Ala
290 295 300
Cys Asn Ala Cys Ser Lys Glu Asp Leu Val Cys Pro Arg Thr Leu Leu
305 310 315 320
Gln Asn Val Val Trp Thr Ser Leu Tyr Lys Trp Val Glu Pro Val Leu
325 330 335
Gly Ser Arg Pro Met Asn Lys Leu Arg Glu Arg Ala Leu Asp Arg Leu
340 345 350
Met Glu His Ile His Tyr Glu Asp Glu Asn Ser Gln Tyr Leu Cys Leu
355 360 365
Cys Pro Val Asn Lys Ala Leu Asn Met Val Cys Cys Trp Val Glu Asp
370 375 380
Pro Asn Ser Asp Ser Phe Lys Arg His Leu Ala Arg Ile Pro Asp Phe
385 390 395 400
Leu Trp Leu Ser Glu Asp Gly Met Lys Ala Gln Ile Tyr Asp Gly Cys
405 410 415
Gln Ser Trp Glu Thr Ala Phe Ile Ile Gln Ala Phe Cys Ala Thr Asp
420 425 430
Leu Val Asn Glu Tyr Gly Ser Thr Val Arg Arg Ala His Glu Phe Met
435 440 445
Lys Asn Ser Gln Ile Met Arg Asn His Pro Gly Asp Gln Ser Tyr Trp
450 455 460
His Arg His Arg Ser Lys Gly Ser Trp Thr Leu Ser Ser Ala Asp Asn
465 470 475 480
Gly Trp Ala Val Ser Asp Thr Thr Ala Glu Ala Leu Lys Ala Val Leu
485 490 495
Leu Leu Glu Lys Ile Ser Ser Asn Val Val Gly Asp Pro Ile Glu Ile
500 505 510
Glu Arg Leu His Asp Ala Val Asp Cys Leu Leu Ser Phe Val Asn Lys
515 520 525
Asp Gly Thr Leu Ser Thr Tyr Glu Cys Lys Arg Thr Tyr Thr Trp Ile
530 535 540
Glu Val Leu Ser Pro Cys Glu Ser Phe Pro Asn Ile Val Val Asp Tyr
545 550 555 560
Pro Phe Pro Glu Cys Thr Ser Ser Val Leu Gln Ala Leu Val Leu Phe
565 570 575
Lys Gln Leu His Pro Ser Tyr Arg Ile Lys Glu Ile Glu Lys Cys Val
580 585 590
Arg Asn Ala Ala Met Phe Ile Glu Ser Thr Gln Gly Glu Asp Gly Ser
595 600 605
Trp Leu Gly Thr Trp Gly Val Cys Phe Thr Tyr Gly Ala Phe Leu Ser
610 615 620
Val Lys Gly Leu Ile Ala Ala Gly Arg Thr Tyr Glu Asn Ser Ser Ser
625 630 635 640
Ile Arg Lys Ala Cys Asp Phe Ile Leu Ser Lys Gln Leu Asp Thr Gly
645 650 655
Gly Trp Gly Glu Ser Tyr Val Ser Asn Ile Thr Lys Val Tyr Val Asn
660 665 670
Ile Lys Asp Asp Gln Ala His Ala Val Asn Thr Ala Trp Ala Met Leu
675 680 685
Ala Leu Ile Cys Ala Gly Gln Met Glu Arg Asp Pro Ala Pro Leu His
690 695 700
Arg Ala Ala Lys Glu Leu Ile Asn Met Gln Leu Glu Thr Gly Glu Phe
705 710 715 720
Pro Gln Gln Glu His Val Gly Ala Phe Asn Ala Cys Leu Phe Phe Asn
725 730 735
Tyr Pro Asn Tyr Arg Asn Leu Phe Pro Ile Trp Ala Leu Gly Glu Tyr
740 745 750
Cys Arg His Leu His Ser Thr Arg Arg Ala
755 760
<210> 107
<211> 2289
<212> DNA
<213> Zea mays
<400> 107
atgtggaggc tgacggtggc cgagggcggc ggcccgtggc tgcgctcgac gaacggcttc 60
gtggggcggg cggtgtggga gttcgaccct gacctcggta cgccggagga gcgcgacgag 120
gtagagaggg tacgccagga gttctccgac caccgcttcc agaggagaga gtcggccgac 180
ctcctcatgc gcatgcagtg cgcaaagcag aacagatctc aacgtcgtga tctgccacgc 240
atcaagcttg gggaggatga gcacgtcact gaagaaattg tactgagctc cttgaggtcg 300
gctctggacc agttctcttc actgcaagcc agcgatgggc actggcctgg tgatttcagc 360
gggattatgt tcatcatgcc tggtttgata tttgccttgt atgtcactgg atcactgaat 420
gttgtcatat caccagaaca tcggcatgag atttgccgct acatctacaa ccaccagaac 480
gaagatggtg gatggggcac acttatcctg ggttcgagca ccatgtttgg cacatgctca 540
aactacatca ccttgaggct tctcggtgag gagccatacg ccaacaacag tgtgttggct 600
aaagggcgtg cttggattct atcccatggt ggtgcaacct tgattcctca gtggggaaaa 660
atatggcttt cggtacttgg attgtttgat tggtcaggaa ataatccaat tttccctgaa 720
ctatggtcca ttccccagtt tcttccattt catccaggga aattctggtg ctttgcccgt 780
atggtatatc taccaatggc ttatctttat ggcaagaaat ttgttggacc aattacacca 840
actatattgg cactaagaga ggaaatctat gacactcctt atggaaagat tgactggagt 900
gatgctcgta gtaaatgcgc aaaggaggac ctcatctgtc cacgcacact gttgcagaat 960
gttatttgga cttcacttta taggtgtgtg gaaccagtat tgagcagttg gcctatcaac 1020
aagctgagag agagagctct gggaaacatc atggagcata tccattatga agatgagaac 1080
acacaatacc tatgcatatg tcctgtgaat aaggctctaa acatggtctg ctgctgggta 1140
gaagatccaa attcagatgc attcaagcgt caccttgcaa ggataccaga cttcttgtgg 1200
atttcagaag atggcatgaa ggcacaggta tatgatggct gccagagctg ggagacatca 1260
ttcataattc aagcattttg cgctacagat cttgttaatg actatggttc aactcttcag 1320
agagcctatg agtttatgaa aaattcacag gtcatgagga accatcctgg tgaccaacgt 1380
tattggcatc gccatagatc gaagggttca tggacacttt catctgcaga caatggatgg 1440
gctgtatctg acactacagg agaagcactt aaggctgtac tgttgctgtc aaagatctca 1500
aacaaaaaca accttgttgg ggatccaata gaaagagaaa ggttgcatga cgctattgat 1560
tgccttctat cttttgcgaa caaagatggc accttttcta catatgagtg caaaagaact 1620
tattcttggt tagagattct aagtccttgt gagactttcc caaacattgt tgtcgattac 1680
ccttacccag aatgcacttc atcagtgctc caagctctga tattgttcaa agacttatat 1740
cctggttacc gcacagaaga gatagaagca ttagttagaa gtgcagcaac gtttattgag 1800
accaaacaac aagaagacgg ttcatggtta ggtaattggg gtatatgttt cacctacggg 1860
gccttctttt cgattaaagg tttagtcgct tctggaagaa cgtacaagaa cagcccttgc 1920
atacggaaag catgccactt catattgtcg aagcagctca gtactggtgg atggggagaa 1980
agtcatatcg ctattgaaac tcaggtgtat gtaaatctca aaggtgaccg tgctcacgct 2040
gtgaataccg cctgggcaat gctcgcttta atttatgctg gacagtttga acgagatcca 2100
acgccattac atcgtgctgc aaaggaattg atcaacatgc aactggagac aggagagttt 2160
cctcagcaag agcatgtagg atgcttcaac tgcagcctct acttcaatta cccaagctac 2220
cgcaacttgt tccccatctg ggctctcggt gagtaccatc gtggccttcg tgcaaagaaa 2280
gacaattga 2289
<210> 108
<211> 762
<212> PRT
<213> Zea mays
<400> 108
Met Trp Arg Leu Thr Val Ala Glu Gly Gly Gly Pro Trp Leu Arg Ser
1 5 10 15
Thr Asn Gly Phe Val Gly Arg Ala Val Trp Glu Phe Asp Pro Asp Leu
20 25 30
Gly Thr Pro Glu Glu Arg Asp Glu Val Glu Arg Val Arg Gln Glu Phe
35 40 45
Ser Asp His Arg Phe Gln Arg Arg Glu Ser Ala Asp Leu Leu Met Arg
50 55 60
Met Gln Cys Ala Lys Gln Asn Arg Ser Gln Arg Arg Asp Leu Pro Arg
65 70 75 80
Ile Lys Leu Gly Glu Asp Glu His Val Thr Glu Glu Ile Val Leu Ser
85 90 95
Ser Leu Arg Ser Ala Leu Asp Gln Phe Ser Ser Leu Gln Ala Ser Asp
100 105 110
Gly His Trp Pro Gly Asp Phe Ser Gly Ile Met Phe Ile Met Pro Gly
115 120 125
Leu Ile Phe Ala Leu Tyr Val Thr Gly Ser Leu Asn Val Val Ile Ser
130 135 140
Pro Glu His Arg His Glu Ile Cys Arg Tyr Ile Tyr Asn His Gln Asn
145 150 155 160
Glu Asp Gly Gly Trp Gly Thr Leu Ile Leu Gly Ser Ser Thr Met Phe
165 170 175
Gly Thr Cys Ser Asn Tyr Ile Thr Leu Arg Leu Leu Gly Glu Glu Pro
180 185 190
Tyr Ala Asn Asn Ser Val Leu Ala Lys Gly Arg Ala Trp Ile Leu Ser
195 200 205
His Gly Gly Ala Thr Leu Ile Pro Gln Trp Gly Lys Ile Trp Leu Ser
210 215 220
Val Leu Gly Leu Phe Asp Trp Ser Gly Asn Asn Pro Ile Phe Pro Glu
225 230 235 240
Leu Trp Ser Ile Pro Gln Phe Leu Pro Phe His Pro Gly Lys Phe Trp
245 250 255
Cys Phe Ala Arg Met Val Tyr Leu Pro Met Ala Tyr Leu Tyr Gly Lys
260 265 270
Lys Phe Val Gly Pro Ile Thr Pro Thr Ile Leu Ala Leu Arg Glu Glu
275 280 285
Ile Tyr Asp Thr Pro Tyr Gly Lys Ile Asp Trp Ser Asp Ala Arg Ser
290 295 300
Lys Cys Ala Lys Glu Asp Leu Ile Cys Pro Arg Thr Leu Leu Gln Asn
305 310 315 320
Val Ile Trp Thr Ser Leu Tyr Arg Cys Val Glu Pro Val Leu Ser Ser
325 330 335
Trp Pro Ile Asn Lys Leu Arg Glu Arg Ala Leu Gly Asn Ile Met Glu
340 345 350
His Ile His Tyr Glu Asp Glu Asn Thr Gln Tyr Leu Cys Ile Cys Pro
355 360 365
Val Asn Lys Ala Leu Asn Met Val Cys Cys Trp Val Glu Asp Pro Asn
370 375 380
Ser Asp Ala Phe Lys Arg His Leu Ala Arg Ile Pro Asp Phe Leu Trp
385 390 395 400
Ile Ser Glu Asp Gly Met Lys Ala Gln Val Tyr Asp Gly Cys Gln Ser
405 410 415
Trp Glu Thr Ser Phe Ile Ile Gln Ala Phe Cys Ala Thr Asp Leu Val
420 425 430
Asn Asp Tyr Gly Ser Thr Leu Gln Arg Ala Tyr Glu Phe Met Lys Asn
435 440 445
Ser Gln Val Met Arg Asn His Pro Gly Asp Gln Arg Tyr Trp His Arg
450 455 460
His Arg Ser Lys Gly Ser Trp Thr Leu Ser Ser Ala Asp Asn Gly Trp
465 470 475 480
Ala Val Ser Asp Thr Thr Gly Glu Ala Leu Lys Ala Val Leu Leu Leu
485 490 495
Ser Lys Ile Ser Asn Lys Asn Asn Leu Val Gly Asp Pro Ile Glu Arg
500 505 510
Glu Arg Leu His Asp Ala Ile Asp Cys Leu Leu Ser Phe Ala Asn Lys
515 520 525
Asp Gly Thr Phe Ser Thr Tyr Glu Cys Lys Arg Thr Tyr Ser Trp Leu
530 535 540
Glu Ile Leu Ser Pro Cys Glu Thr Phe Pro Asn Ile Val Val Asp Tyr
545 550 555 560
Pro Tyr Pro Glu Cys Thr Ser Ser Val Leu Gln Ala Leu Ile Leu Phe
565 570 575
Lys Asp Leu Tyr Pro Gly Tyr Arg Thr Glu Glu Ile Glu Ala Leu Val
580 585 590
Arg Ser Ala Ala Thr Phe Ile Glu Thr Lys Gln Gln Glu Asp Gly Ser
595 600 605
Trp Leu Gly Asn Trp Gly Ile Cys Phe Thr Tyr Gly Ala Phe Phe Ser
610 615 620
Ile Lys Gly Leu Val Ala Ser Gly Arg Thr Tyr Lys Asn Ser Pro Cys
625 630 635 640
Ile Arg Lys Ala Cys His Phe Ile Leu Ser Lys Gln Leu Ser Thr Gly
645 650 655
Gly Trp Gly Glu Ser His Ile Ala Ile Glu Thr Gln Val Tyr Val Asn
660 665 670
Leu Lys Gly Asp Arg Ala His Ala Val Asn Thr Ala Trp Ala Met Leu
675 680 685
Ala Leu Ile Tyr Ala Gly Gln Phe Glu Arg Asp Pro Thr Pro Leu His
690 695 700
Arg Ala Ala Lys Glu Leu Ile Asn Met Gln Leu Glu Thr Gly Glu Phe
705 710 715 720
Pro Gln Gln Glu His Val Gly Cys Phe Asn Cys Ser Leu Tyr Phe Asn
725 730 735
Tyr Pro Ser Tyr Arg Asn Leu Phe Pro Ile Trp Ala Leu Gly Glu Tyr
740 745 750
His Arg Gly Leu Arg Ala Lys Lys Asp Asn
755 760
<210> 109
<211> 1149
<212> DNA
<213> Sorghum bicolor
<400> 109
atgtggaggc tcaaggtcgc ggagggcgca gggccatggc taaagtcatc caacaacttc 60
cgtgggcgag ctgtttggga gttcgacccg gagctcggca cgccggagga gcgtgccgag 120
gtggagaggg tgcgccgtga gttcaccgag cgccgctttg agaaaagaga gtcctctgac 180
ctcctcctgc aaatgcagta tgcaaaacat aaacatcttc aggtggatcc tccaccggcc 240
acactcgcag aaaatgaaga agtcacagag gagatcatat tagcagcatt gaagcgagcc 300
ctcgctcaac attcaagtct gcaagcagat gatgggtgct ggccagcaga ttttagtggc 360
attttattca ttatgcctct cctgaatgaa gatggtggtt ggggtaaaca agtgttggga 420
ccaagcacca tgtttggctc atgcttaaac tatgttgcct taagacttat tggtgaagag 480
cgcaaaaatg ttgcattgac caaaggacgt gagtggattt tgtcccatgg aagtgcaact 540
gcaattccgc agtggggaaa gatatggttc tcggtgatgg gtttgtatga ttggtctggc 600
aataatccaa taattccaga gttatggctt gtcccacact ttcttccaat tcatccagga 660
cgattttggg tttactgccg tatggtgtat ctgcctatgg cttacctctt tgctaaaaaa 720
ttcgttggaa caattacacc aactatactg gaattaaggg acgagctcta tagtatacca 780
tacagtgaga ttgattggaa agaagctcgt gatacttgtg ccaaggtgga ccttgtatat 840
ccaaggacaa tggcacaaaa tcttgtatgg acctgcctta ataaagtcat agagccagta 900
ctgaattgtt ggccattcaa caagttaaga gatatagcat tgaaaaacat tatggaacat 960
atccactatg aagatgaaac tagtaaatac atctgtgtat gtcccataaa caaggcacta 1020
gatatgattt gttgttgggc agaaaatcca gattcagatg cattcaagca gcatcttcca 1080
aggatatatg attttttatg gcttgcagaa gatggaatga aggcacaggt taaaaagatc 1140
aaaacatag 1149
<210> 110
<211> 382
<212> PRT
<213> Sorghum bicolor
<400> 110
Met Trp Arg Leu Lys Val Ala Glu Gly Ala Gly Pro Trp Leu Lys Ser
1 5 10 15
Ser Asn Asn Phe Arg Gly Arg Ala Val Trp Glu Phe Asp Pro Glu Leu
20 25 30
Gly Thr Pro Glu Glu Arg Ala Glu Val Glu Arg Val Arg Arg Glu Phe
35 40 45
Thr Glu Arg Arg Phe Glu Lys Arg Glu Ser Ser Asp Leu Leu Leu Gln
50 55 60
Met Gln Tyr Ala Lys His Lys His Leu Gln Val Asp Pro Pro Pro Ala
65 70 75 80
Thr Leu Ala Glu Asn Glu Glu Val Thr Glu Glu Ile Ile Leu Ala Ala
85 90 95
Leu Lys Arg Ala Leu Ala Gln His Ser Ser Leu Gln Ala Asp Asp Gly
100 105 110
Cys Trp Pro Ala Asp Phe Ser Gly Ile Leu Phe Ile Met Pro Leu Leu
115 120 125
Asn Glu Asp Gly Gly Trp Gly Lys Gln Val Leu Gly Pro Ser Thr Met
130 135 140
Phe Gly Ser Cys Leu Asn Tyr Val Ala Leu Arg Leu Ile Gly Glu Glu
145 150 155 160
Arg Lys Asn Val Ala Leu Thr Lys Gly Arg Glu Trp Ile Leu Ser His
165 170 175
Gly Ser Ala Thr Ala Ile Pro Gln Trp Gly Lys Ile Trp Phe Ser Val
180 185 190
Met Gly Leu Tyr Asp Trp Ser Gly Asn Asn Pro Ile Ile Pro Glu Leu
195 200 205
Trp Leu Val Pro His Phe Leu Pro Ile His Pro Gly Arg Phe Trp Val
210 215 220
Tyr Cys Arg Met Val Tyr Leu Pro Met Ala Tyr Leu Phe Ala Lys Lys
225 230 235 240
Phe Val Gly Thr Ile Thr Pro Thr Ile Leu Glu Leu Arg Asp Glu Leu
245 250 255
Tyr Ser Ile Pro Tyr Ser Glu Ile Asp Trp Lys Glu Ala Arg Asp Thr
260 265 270
Cys Ala Lys Val Asp Leu Val Tyr Pro Arg Thr Met Ala Gln Asn Leu
275 280 285
Val Trp Thr Cys Leu Asn Lys Val Ile Glu Pro Val Leu Asn Cys Trp
290 295 300
Pro Phe Asn Lys Leu Arg Asp Ile Ala Leu Lys Asn Ile Met Glu His
305 310 315 320
Ile His Tyr Glu Asp Glu Thr Ser Lys Tyr Ile Cys Val Cys Pro Ile
325 330 335
Asn Lys Ala Leu Asp Met Ile Cys Cys Trp Ala Glu Asn Pro Asp Ser
340 345 350
Asp Ala Phe Lys Gln His Leu Pro Arg Ile Tyr Asp Phe Leu Trp Leu
355 360 365
Ala Glu Asp Gly Met Lys Ala Gln Val Lys Lys Ile Lys Thr
370 375 380
<210> 111
<211> 2274
<212> DNA
<213> Glycine max
<400> 111
atgtggaagc tcaagttcgc cgaaggaggg aatccatggc ttcggacatt gaacaatcac 60
gttggaagac aggtgtggga gttcgatcct aagcttggat cgccgcaaga tctcctcgag 120
attgagaaag ctcgccagaa ttttcacgat aaccgcttta cccacaaaca cagcgctgat 180
ctacttatgc ggatgcagtt cgcaagagag aacccaacac gtgaagtctt gcccaaagtc 240
ggagttaagg atattgagga tgtgacccaa gagattgtga caaaaacatt aagaagggcc 300
gtaagtttcc attcaactct ccagtgccat gacggacact ggccgggaga ttatggaggt 360
cccatgtttc tgatgcctgg cttggtaatt actctgtcta tcactggggc gttgaataca 420
gtcttaactg aagaacatag aaaggaaata tgccgttacc tctataatca tcaaaacaag 480
gatggtgggt ggggtttgca tattgaaggt ccaagcacca tgtttggctc tgtcttgagt 540
tatattactc tgagattgct aggtgagggg cctaatgatg gacaagggga aatggagaag 600
gcacgtgact ggattctagg gcatggtggt gctacttata taacgtcatg ggggaagatg 660
tggctttcag tacttggagt gtatgaatgg tctggaaata atcccctgcc ccctgagata 720
tggctccttc catacatgct tccatttcat ccaggaagga tgtggtgtca ctgccggatg 780
gtctatttgc cgatgtccta cttatatggc aagaggtttg ttggtccaat ctcaccaaca 840
gtattatctt tgagaaaaga gctttataca gtaccatacc atgatataga ttgggatcag 900
gctcgcaatt tgtgtgcaaa ggaagatttg tactatcctc acccacttgt acaggatatt 960
ctttgggcat ctctacacaa gttccttgag cctattctga tgcattggcc tggaaaaaga 1020
ttgagggaaa aggctattat ttctgcattg gagcatatac attacgaaga tgagaatact 1080
cgatatattt gcataggtcc tgtaaataag gtgttaaata tgctttgctg ttgggtggaa 1140
gatccaaatt ctgaggcctt caagttgcat cttcccagga tttatgatta tctatggatt 1200
gcagaagatg gcatgaaaat gcagggctac aatggaagtc aactatggga cactgctttt 1260
gctgtccaag caattattgc atctaacctc attgaagaat ttggtccaac tataagaaaa 1320
gctcatacct atattaagaa ttcacaggtt ttagaagatt gtccaggtga tcttaataaa 1380
tggtaccgtc acatttcaaa aggtgcttgg cctttttcaa ctggagatca tggatggcca 1440
atttctgact gcacagctga aggactgaaa gctgttctat tactatccaa aattgcacca 1500
gaaatagttg gtgagccaat agacgtgaag cgattatatg attctgtaaa tgtcattctc 1560
tcactacaga atgaagatgg tggttttgca acatatgagc ttaaacgatc ttataattgg 1620
ttggagataa tcaatcctgc tgaaactttt ggtgacatcg ttattgatta tccttatgtg 1680
gaatgtacat cagcagcgat tcaagctttg gcatcattta ggaaattata tcctgggcat 1740
cgccgagaag aaatacaaca ttgtatcgat aaagccacta ccttcattga aaaaatacaa 1800
gcttcagatg gatcatggta tggttcttgg ggagtttgct tcacttacgg tgcttggttt 1860
ggggtaaaag gtctgattgc tgctggaagg agtttcagta attgctcaag catccgtaaa 1920
gcttgtgaat ttctgctgtc caagcagctt ccttctggtg gctggggaga gagttatctg 1980
tcctgtcaaa acaaggtgta ttcaaatctg gaaggcaaca ggtctcatgt ggtcaacact 2040
gggtgggcta tgttggctct cattgatgct ggacaggcta agagagattc gcaaccactg 2100
caccgggcag ctgcatactt gataaattcc caattggagg acggtgactt tccgcagcag 2160
gaaataatgg gagtcttcaa caagaattgc atgatcacat acgccgcata cagaaacata 2220
ttccccattt gggcgttggg agaataccaa tcccaagtat tgcaatctcg ttaa 2274
<210> 112
<211> 757
<212> PRT
<213> Glycine max
<400> 112
Met Trp Lys Leu Lys Phe Ala Glu Gly Gly Asn Pro Trp Leu Arg Thr
1 5 10 15
Leu Asn Asn His Val Gly Arg Gln Val Trp Glu Phe Asp Pro Lys Leu
20 25 30
Gly Ser Pro Gln Asp Leu Leu Glu Ile Glu Lys Ala Arg Gln Asn Phe
35 40 45
His Asp Asn Arg Phe Thr His Lys His Ser Ala Asp Leu Leu Met Arg
50 55 60
Met Gln Phe Ala Arg Glu Asn Pro Thr Arg Glu Val Leu Pro Lys Val
65 70 75 80
Gly Val Lys Asp Ile Glu Asp Val Thr Gln Glu Ile Val Thr Lys Thr
85 90 95
Leu Arg Arg Ala Val Ser Phe His Ser Thr Leu Gln Cys His Asp Gly
100 105 110
His Trp Pro Gly Asp Tyr Gly Gly Pro Met Phe Leu Met Pro Gly Leu
115 120 125
Val Ile Thr Leu Ser Ile Thr Gly Ala Leu Asn Thr Val Leu Thr Glu
130 135 140
Glu His Arg Lys Glu Ile Cys Arg Tyr Leu Tyr Asn His Gln Asn Lys
145 150 155 160
Asp Gly Gly Trp Gly Leu His Ile Glu Gly Pro Ser Thr Met Phe Gly
165 170 175
Ser Val Leu Ser Tyr Ile Thr Leu Arg Leu Leu Gly Glu Gly Pro Asn
180 185 190
Asp Gly Gln Gly Glu Met Glu Lys Ala Arg Asp Trp Ile Leu Gly His
195 200 205
Gly Gly Ala Thr Tyr Ile Thr Ser Trp Gly Lys Met Trp Leu Ser Val
210 215 220
Leu Gly Val Tyr Glu Trp Ser Gly Asn Asn Pro Leu Pro Pro Glu Ile
225 230 235 240
Trp Leu Leu Pro Tyr Met Leu Pro Phe His Pro Gly Arg Met Trp Cys
245 250 255
His Cys Arg Met Val Tyr Leu Pro Met Ser Tyr Leu Tyr Gly Lys Arg
260 265 270
Phe Val Gly Pro Ile Ser Pro Thr Val Leu Ser Leu Arg Lys Glu Leu
275 280 285
Tyr Thr Val Pro Tyr His Asp Ile Asp Trp Asp Gln Ala Arg Asn Leu
290 295 300
Cys Ala Lys Glu Asp Leu Tyr Tyr Pro His Pro Leu Val Gln Asp Ile
305 310 315 320
Leu Trp Ala Ser Leu His Lys Phe Leu Glu Pro Ile Leu Met His Trp
325 330 335
Pro Gly Lys Arg Leu Arg Glu Lys Ala Ile Ile Ser Ala Leu Glu His
340 345 350
Ile His Tyr Glu Asp Glu Asn Thr Arg Tyr Ile Cys Ile Gly Pro Val
355 360 365
Asn Lys Val Leu Asn Met Leu Cys Cys Trp Val Glu Asp Pro Asn Ser
370 375 380
Glu Ala Phe Lys Leu His Leu Pro Arg Ile Tyr Asp Tyr Leu Trp Ile
385 390 395 400
Ala Glu Asp Gly Met Lys Met Gln Gly Tyr Asn Gly Ser Gln Leu Trp
405 410 415
Asp Thr Ala Phe Ala Val Gln Ala Ile Ile Ala Ser Asn Leu Ile Glu
420 425 430
Glu Phe Gly Pro Thr Ile Arg Lys Ala His Thr Tyr Ile Lys Asn Ser
435 440 445
Gln Val Leu Glu Asp Cys Pro Gly Asp Leu Asn Lys Trp Tyr Arg His
450 455 460
Ile Ser Lys Gly Ala Trp Pro Phe Ser Thr Gly Asp His Gly Trp Pro
465 470 475 480
Ile Ser Asp Cys Thr Ala Glu Gly Leu Lys Ala Val Leu Leu Leu Ser
485 490 495
Lys Ile Ala Pro Glu Ile Val Gly Glu Pro Ile Asp Val Lys Arg Leu
500 505 510
Tyr Asp Ser Val Asn Val Ile Leu Ser Leu Gln Asn Glu Asp Gly Gly
515 520 525
Phe Ala Thr Tyr Glu Leu Lys Arg Ser Tyr Asn Trp Leu Glu Ile Ile
530 535 540
Asn Pro Ala Glu Thr Phe Gly Asp Ile Val Ile Asp Tyr Pro Tyr Val
545 550 555 560
Glu Cys Thr Ser Ala Ala Ile Gln Ala Leu Ala Ser Phe Arg Lys Leu
565 570 575
Tyr Pro Gly His Arg Arg Glu Glu Ile Gln His Cys Ile Asp Lys Ala
580 585 590
Thr Thr Phe Ile Glu Lys Ile Gln Ala Ser Asp Gly Ser Trp Tyr Gly
595 600 605
Ser Trp Gly Val Cys Phe Thr Tyr Gly Ala Trp Phe Gly Val Lys Gly
610 615 620
Leu Ile Ala Ala Gly Arg Ser Phe Ser Asn Cys Ser Ser Ile Arg Lys
625 630 635 640
Ala Cys Glu Phe Leu Leu Ser Lys Gln Leu Pro Ser Gly Gly Trp Gly
645 650 655
Glu Ser Tyr Leu Ser Cys Gln Asn Lys Val Tyr Ser Asn Leu Glu Gly
660 665 670
Asn Arg Ser His Val Val Asn Thr Gly Trp Ala Met Leu Ala Leu Ile
675 680 685
Asp Ala Gly Gln Ala Lys Arg Asp Ser Gln Pro Leu His Arg Ala Ala
690 695 700
Ala Tyr Leu Ile Asn Ser Gln Leu Glu Asp Gly Asp Phe Pro Gln Gln
705 710 715 720
Glu Ile Met Gly Val Phe Asn Lys Asn Cys Met Ile Thr Tyr Ala Ala
725 730 735
Tyr Arg Asn Ile Phe Pro Ile Trp Ala Leu Gly Glu Tyr Gln Ser Gln
740 745 750
Val Leu Gln Ser Arg
755

Claims (19)

1.一种分离的多核苷酸，其包含的多核苷酸编码的多肽的氨基酸序列为与SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112有至少90％序列一致性的序列，其中植物中所述多核苷酸表达的增加能提高耐旱性和产量。
2.权利要求1所述的分离的多核苷酸，其中所述多核苷酸包含的核苷酸序列为SEQ IDNO:1、SEQ ID NO:2、SEQ ID NO:4、SEQ ID NO:5、SEQ ID NO:7、SEQ ID NO:8、SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:13、SEQ ID NO:14、SEQ ID NO:16、SEQ ID NO:17、SEQ IDNO:19、SEQ ID NO:20、SEQ ID NO:22、SEQ ID NO:23.SEQ ID NO:25、SEQ ID NO:26、SEQ IDNO:28、SEQ ID NO:29、SEQ ID NO:53、SEQ ID NO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ IDNO:61、SEQ ID NO:63、SEQ ID NO:65、SEQ ID NO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ IDNO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ ID NO:79、SEQ ID NO:81.SEQ ID NO:83、SEQ IDNO:85、SEQ ID NO:87、SEQ ID NO:89、SEQ ID NO:91.SEQ ID NO:93、SEQ ID NO:95、SEQ IDNO:97、SEQ ID NO:99、SEQ ID NO:101.SEQ ID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109或SEQ ID NO:111。
3.权利要求1所述的分离的多核苷酸，其中所述编码多肽包含的氨基酸序列为SEQ IDNO:3、3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112。
4.权利要求1-3中任何分离的多核苷酸，干旱条件下，植物中该多核苷酸表达的增加能提高耐旱性和/或籽粒产量。
5.包含有权利要求1-3中任何分离的多核苷酸的重组DNA构建体，可操作地连接至少一个异源调控元件。
6.权利要求5中的重组DNA构建体，其中所述调控元件是一个异源启动子。
7.一种改良的植物或种子，含有增加表达的至少一种编码多肽的多核苷酸，所述多肽含有的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112这些序列有至少90％的序列一致性。
8.权利要求7所述的植物，其中该植物在其基因组中包含有一种重组DNA构建体，所述重组DNA构建体含有权利要求1到3中任一可操作地连接至少一个调控元件的多核苷酸，其中所述植物与对照植物相比较时，表现出提高的耐旱性。
9.权利要求7所述的植物，该植物在一个基因组位点上包含有一个靶向基因修饰，所述靶向基因修饰的基因组位点含有的多核苷酸序列编码的多肽的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112这些序列有至少90％的序列一致性。因而增加该多肽的表达，所述植物在与对照植物相比较时，表现出提高的耐旱性。
10.权利要求7-9所述的任何植物，其中所述植物选自水稻、玉米、大豆、向日葵、高粱、油菜、小麦、苜蓿、棉花、大麦、小米、甘蔗和柳枝稷。
11.一种增加植物耐旱性的方法，包括增加至少一个编码多肽的多核苷酸的表达，所述多肽含有的氨基酸序列与SEQ ID NO：3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112这些序列有至少90％的序列一致性。
12.权利要求11所述的方法，其中该方法包括：
a)在一个可再生植物细胞中表达重组DNA构建体，该重组DNA构建体含有一个调控元件可操作地连接所述多核苷酸序列；和
b)再生所述植物，其中该植物在其基因组中包含有该重组DNA构建体。
13.权利要求11所述的方法，其中该方法包括：
a)向一个可再生植物细胞的基因组位点引入一个靶向基因修饰，所述靶向基因修饰的基因组位点编码含有氨基酸序列的多肽，所述氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112这些序列相比较时有至少90％的序列一致性；和
b)再生所述植物，其中该植物中所述多肽的表达水平和/或活性是增加的。
14.权利要求13所述的方法，其中所述靶向基因修饰的引入使用的基因修饰技术选自多核苷酸引导的核酸内切酶、CRISPR-Cas核酸内切酶、碱基编辑脱氨酶、锌指核酸酶、转录激活物样效应器核酸酶(TALEN)、工程化位点特异性巨核酸酶或Argonaute。
15.权利要求13所述的方法，其中所述靶向基因修饰存在于基因组位点的(a)编码区；(b)非编码区；(c)调控序列；(d)未翻译区；或(e)任何(a)-(d)的组合，以编码含有氨基酸序列的多肽，该氨基酸序列与选自SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112的这些氨基酸序列有至少90％的一致性。
16.权利要求12所述的方法，其中所述调控元件是一个异源启动子。
17.增加植物耐旱性的方法，所述方法包括：
a)在一个可再生植物细胞中表达一种可操作地连接有至少一个异源调控元件的多核苷酸，其中所述多核苷酸编码的多肽的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21、24、27、30、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110或112有至少90％的序列一致性，且与对照植物相比，该多核苷酸的表达水平是增加的；和
b)与不包含有可操作地连接到异源调节元件的多核苷酸的对照植物相比，选择包含可操作地连接到异源调节元件的多核苷酸的植物以提高耐旱性。
18.权利要求17所述的方法，其中所述异源调控元件是一种启动子。
19.权利要求17或18所述的方法，其中所述植物选自水稻、玉米、大豆、向日葵、油菜、小麦、苜蓿、棉花、大麦、小米、甘蔗和柳枝稷。
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