CN114616333A - 非生物胁迫耐性植物和方法 - Google Patents

非生物胁迫耐性植物和方法 Download PDF

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CN114616333A
CN114616333A CN201980098939.7A CN201980098939A CN114616333A CN 114616333 A CN114616333 A CN 114616333A CN 201980098939 A CN201980098939 A CN 201980098939A CN 114616333 A CN114616333 A CN 114616333A
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吕贵华
王国奎
张玉
毛冠凡
王昌贵
陈光武
刘爱芬
焦荣荣
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Sinobioway Bio Agriculture Group Co Ltd
Pioneer Overseas Corp
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Abstract

提供了分离的多核苷酸，可用于赋予增加的耐旱性和产量。还提供了用于提高植物耐旱性的重组DNA构建体、改良的植物或种子以及方法。

Description

非生物胁迫耐性植物和方法
技术领域
本公开发明涉及到植物育种和遗传学领域，尤其是，涉及到提高植物对非生物胁迫的耐受性。
研究背景
生物和非生物原因可以对植物产生胁迫，例如造成生物胁迫的原因包括病原菌感染、昆虫取食、一种植物对另一种植物的寄生，例如槲寄生；非生物胁迫包括诸如有效水分的过量或者不足、极限温度和诸如除草剂的合成化学药品。
非生物胁迫是造成世界范围内农作物减产的主要原因，造成主要农作物平均减产50％以上(Boyer,J.S.(1982)Science,218：443-448；Bray,E.A.等(2000)In Biochemistryand Molecular Biology of Plants,编辑Buchannan,B.B.等,Amer.Soc.Plant Biol.,pp.1158-1249；Mushtaq等(2018)Journal of Plant Physiology,224–225:156–162)。尽管人们对非生物胁迫响应和抗性进行了广泛的研究，并应用各种技术开发了非生物胁迫耐受作物，但目前还没有理想的商业产品。
因此，需要开发提高植物对非生物胁迫耐受性的成分和方法。本发明提供此类组合物和方法。
发明概述
以下是本发明所涵盖的实施例：
在一个实施例中，本公开发明包括一个分离的多核苷酸，其编码的多肽的氨基酸序列与SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158有至少90％的序列一致性，其中植物中该多核苷酸表达的增加能提高耐旱性。在某些实施例中，所述分离的多核苷酸编码的氨基酸序列是SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126,128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158。在某些实施例中所述分离的多核苷酸含有的核苷酸序列为SEQ ID NO:1、2、4、6、7、9、10、12、13、15、16、18、19、21、22、24、25、27、28、30、31、33、34、36、37、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109、111、113、115、117、119、121、123、125、127、129、131、133、135、137、139、141、143、145、147、149、151、153、155、157。在某些实施例中，植物中该多核苷酸表达的增加提高了干旱条件下籽粒的产量。
本公开发明还提供了一种重组DNA构建体，其含有的分离的多核苷酸可操作地连接至少一个异源调控元件，其中所述多核苷酸编码的多肽的氨基酸序列与SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158有至少90％的序列一致性。
本公开发明进一步提供了一个改良植物或种子，含有至少一个编码多肽的多核苷酸表达或活性的增加，所述多肽的氨基酸序列与SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158有至少90％的序列一致性。在某些实施例中，所述改良的植物或种子在其基因组中含有一个重组DNA构建体，其含有的多核苷酸可操作地连接至少一个异源调控元件，其中所述多核苷酸编码的多肽的氨基酸序列与SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158有至少90％的序列一致性。在某些实施例中，该改良的植物和同样生长在干旱条件下的对照植物相比较，表现出提高的耐旱性和/或增加的籽粒产量。
在某些实施例中，所述改良的植物或种子在其基因组位点上含有一个靶向基因修饰，该靶向基因修饰的基因组位点上含有一种编码多肽的多核苷酸，该多肽的氨基酸序列与SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158有至少90％的序列一致性，其中所述靶向基因修饰提高了该多肽的表达和/或活性。在某些实施例中，所述改良的植物与同样生长在干旱条件下的对照植物相比较，表现出提高的耐旱性和增加的籽粒产量。
在某些实施例中，所述植物选自水稻、玉米、大豆、向日葵、高粱、油菜、小麦、苜蓿、棉花、大麦、小米、甘蔗和柳枝稷。
还提供了一种提高植物耐旱性的方法，该方法包括增加至少一个编码多肽的多核苷酸的表达，所述多肽的氨基酸序列与SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158有至少90％的序列一致性。其中所述植物与对照植物相比较时，表现出提高的耐旱性。
在某些实施例中，所述提高耐旱性的方法包括：(a)向一个可再生的植物细胞引入一个重组DNA构建体，其含有一个多核苷酸可操作地连接至少一个异源调控元件，其中所述多核苷酸编码的多肽的氨基酸序列与SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158相比较时，有至少80％的序列一致性；和(b)再生所述植物，该植物在其基因组中含有所述重组DNA构建体。
在某些实施例中，提高植物耐旱性的方法包括：(a)向一个可再生植物细胞的基因组位点上引入一个靶向基因修饰，该靶向基因修饰的基因组位点上包含一个编码多肽的多核苷酸，，其编码的多肽的氨基酸序列与SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158相比较时，有至少80％的序列一致性；和(b)再生所述植物，该植物在其基因组中包含有引入的基因修饰，且所述多肽的表达和/或活性是增加的。在某些实施例中，靶向基因修饰的引入可使用以下基因组修饰技术：多核苷酸引导的核酸内切酶、CRISPR-Cas核酸内切酶、碱基编辑脱氨酶、锌指核酸酶、转录激活物样效应器核酸酶(TALEN)、工程位点特异性巨核酸酶或Argonaute。在某些实施例中，靶向基因修饰存在于基因组位点的(a)编码区域；(b)非编码区域；(c)调控区域；(d)非翻译区域；或(e)任意(a)-(d)的组合，其编码的多肽的氨基酸序列与SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110,112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146,148、150、152、154、156或158相比较时有至少80％的序列一致性。
附图说明和序列表
通过以下详细描述和构成本申请一部分的随附序列列表，可以更全面地理解本发明。此处所附的序列描述和序列列表符合37C.F.R.§§1.821和1.825中规定的专利申请中核苷酸和氨基酸序列披露规则。序列描述包括37C.F.R.§§1.821和1.825中定义的氨基酸的三个字母代码，通过引用并入本文。
表1.序列表详述
Figure BDA0003493940310000031
Figure BDA0003493940310000041
Figure BDA0003493940310000051
详细描述
本文件所述各参考文献全部通过引用并入本文件。
如本文和所附权利要求中所使用的，除非上下文另有明确规定，否则单数形式“一个”、“一种”和“所述”包括复数引用。因此，例如，对“植物”的引用包括多个这样的植物；对“细胞”的引用包括本领域技术人员已知的一个或多个细胞及其等效物，等等。
定义
在本文中，植物“增加的耐旱性”是指生理或物理特性的任何可测量的改善，例如产量，当在干旱条件下生长时相对于参照或对照植物测量。通常，当基因组中包含重组DNA构建体或DNA修饰的植物，与基因组中不包含重组DNA构建体或DNA修饰的参照或对照植物相比较时，表现出更强的耐旱性。
“农艺性状”是可测量的指标参数，包括但不限于：叶片绿色程度、籽粒产量、生长速率、总生物量或积累速率、成熟时的鲜重、成熟时的干重、果实产量、种子产量、植物总氮含量、果实氮含量、种子氮含量、植物营养组织氮含量、植物总游离氨基酸含量、果实游离氨基酸含量、种子游离氨基酸含量、植物营养组织游离氨基酸含量、植物总蛋白含量、果实蛋白含量、种子蛋白含量、植物营养组织蛋白质含量、耐旱性、氮的吸收、根的倒伏、收获指数、茎的倒伏、株高、穗高、穗长、耐盐性、分蘖数、圆锥花序的大小、早期苗的活力和低温胁迫下的出苗状况。
“转基因的”指其基因组因异源核酸(如重组DNA构建体)的存在而发生改变的任何细胞、细胞系、愈伤组织、组织、植物部分或植物。例如一个重组DNA构建体，包括最初的转基因事件以及由最初转基因事件产生的有性杂交或无性繁殖产生的事件。本文使用的术语“转基因”不包括通过常规植物育种方法或自然发生的事件(如随机交叉受精、非重组病毒感染、非重组细菌转化、非重组转座)改变基因组(染色体或额外染色体)，或自发突变。
“对照”、“对照植物”或“对照植物细胞”为测定受测试植物或植物细胞的表型变化提供参考，由于转化，受测植物或植物细胞的基因组改变影响到目的基因，测试的植物或植物细胞可以是转基因植物或转基因植物细胞的子代。例如，除了导致测试植物或细胞的遗传改变之外，对照植物可以和测试植物具有相同的遗传背景。
“植物”包括整个植株、植物器官、植物组织、种子和植物细胞以及该植株的子代。植物细胞包括但不限于来自下列物质的细胞：种子、悬浮培养物、胚、分生区域、愈伤组织、叶、根、芽、配子体、孢子体、花粉和小孢子。
“子代”包括植物的任何后续世代。
“修饰植物”包括在其基因组内包含异源多核苷酸或修饰基因或启动子的植物。例如，异源多核苷酸稳定地整合在基因组内，使得该多核苷酸被传递到连续世代。异源多核苷酸可单独或作为重组DNA构建物的一部分整合到基因组中。
关于序列的“异源”意思是来源于外来物种的序列，或者，如果来自同一物种，在组成和/或基因组位置上通过人为干预对其天然的组成形式进行了实质性的修改。
“多核苷酸”、“核酸序列”、“核苷酸序列”或“核酸片段”可互换使用并且是任选含有合成的、非天然的或改变的核苷酸碱基的单链或双链RNA或DNA聚合物。核苷酸(通常以它们的5′-单磷酸形式存在)通过如下它们的单个字母名称来指代：“A”为腺苷酸或脱氧腺苷酸(分别对应RNA或DNA)，“C”表示胞苷酸或脱氧胞苷酸，“G”表示鸟苷酸或脱氧鸟苷酸，“U”表示尿苷酸，“T”表示脱氧胸苷酸，“R”表示嘌呤(A或G)，“Y”表示嘧啶(C或T)，“K”表示G或T，“H”表示A或C或T，“I”表示肌苷，并且“N”表示任何核苷酸。
“多肽”、“肽”、“氨基酸序列”和“蛋白质”在本发明中可互换使用，指氨基酸残基的聚合物。该术语适用于其中一个或多个氨基酸残基是相应的天然存在的氨基酸的人工化学类似物的氨基酸聚合物，以及适用于天然存在的氨基酸聚合物。术语“多肽”、“肽”、“氨基酸序列”和“蛋白质”还可包括修饰形式，包括但不限于糖基化、脂质连接、硫酸盐化、谷氨酸残基的γ羧化、羟化和ADP-核糖基化。
“重组DNA构建体”指在自然界中通常不会一起存在的核酸片段的组合。因此，重组DNA构建体可包含源于不同来源的调控序列和编码序列，或源于相同来源但以不同于通常天然存在的方式排列的调控序列和编码序列。
“调控元件”指位于编码序列的上游(5'非编码序列)、中间或下游(3'非编码序列)，并且影响相关编码序列的转录、RNA 加工或稳定性或者翻译的核苷酸序列。调控序列可包括但不限于启动子、翻译前导序列、内含子和多腺苷酸化识别序列。术语“调控序列”和“调控元件”在本文中可互换使用。
“启动子”指能够控制另一核酸片段转录的核酸片段。“植物中有功能启动子”是能够控制植物细胞中基因转录的启动子，无论其是否来源于植物细胞。“组织特异性启动子”和“组织优选启动子”可互换使用，并且指主要但非必须专一地在一种组织或器官中表达，而且也可在一种特定细胞或细胞型中表达的启动子。“发育调控启动子”指其活性由发育事件决定的启动子。
“可操作地连接”指核酸片段连接成单一片段，使得其中一个核酸片段的功能受到另一个核酸片段的调控。例如，在启动子能够调节核酸片段的转录时，该启动子与该核酸片段进行了可操作地连接。
“表达”指功能产物的产生。例如，核酸片段的表达可指核酸片段的转录(如转录生成mRNA或功能RNA)和/或RNA翻译成前体或成熟蛋白质。
在本文中，“增加”、“增多”等是指实验组(例如，具有本文所述DNA修饰的植物)与对照组(例如，不包含DNA修饰的野生型植物)相比的任何可检测到的增加。因此，蛋白质的增加表达包括样品中蛋白质总水平的任何可检测的增加，并且可以使用本领域的常规方法(例如，Western blotting和ELISA)来确定。
在本文中，“产量”指每单位土地收获的农业产量，可包括收获时作物每英亩蒲式耳或每亩千克数，并根据谷物水分进行调整(例如，玉米通常为15％，水稻为13.5％)。谷物水分是在谷物收获时测量的。谷物的调整试验重量确定为每蒲式耳磅或每株克的重量，并根据收获时的谷物水分水平进行调整。
在本文中，在两个多核苷酸或多肽序列的上下文中，“序列一致性”或“一致性”指当在指定的比较窗口上对齐以获得最大对应性时相同的两个序列中的残基。当序列同一性百分比用于蛋白质时，人们认识到，不完全相同的残基位置往往因保守的氨基酸替换而不同，氨基酸残基被具有类似化学性质(如电荷或疏水性)的其他氨基酸残基取代，因此不会改变分子的功能性质。当序列在保守替换中不同时，可向上调整序列一致性百分比，以纠正替换的保守性质。通过这种保守替换而不同的序列称为“序列相似性”或“相似性”。进行此调整的方法为本领域技术人员所熟知。通常，这涉及将保守替换作为部分不匹配而不是完全不匹配进行评分，从而增加序列一致性的百分比。因此，例如，如果相同氨基酸的得分为1，非保守替代的得分为0，保守替代的得分为0到1之间。计算保守替换的得分，例如，在PC/GENE计划(Intelligenetics,Mountain View,California)中实施。
“序列一致性百分比(％)”是经过序列比对和引入缺口后，待测序列的序列一致性计算包括，将两个序列中相同核苷酸碱基或氨基酸残基的位置数目，获得匹配位置数，然后将匹配的位置数除以比对窗口中位置总数再乘以100。
除非另有说明，本文提供的序列的多重比对是使用Clustal V比对方法(Higginsand Sharp.(1989)CABIOS,5:151-153)进行的，具有默认参数(间隙惩罚＝10，间隙长度惩罚＝10)。使用Clustal V方法进行成对比对和氨基酸序列同一性百分比计算的默认参数为K倍数＝1，间隙惩罚＝3，窗口＝5，对角线保存＝5。对于核酸，这些参数为K倍数＝2，间隙惩罚＝5，窗口＝4，对角线保存＝4。序列对齐后，使用Clustal V程序，通过查看同一程序上的“序列距离”表，可以获得“一致性百分比”和“散度”值；除非另有说明，否则本文中提供和声明和分歧百分比是以这种方式计算的。
组合物：
A.多核苷酸和多肽
本公开发明提供的多核苷酸编码以下多肽：FBX335(OsFBX335-F-box结构域蛋白)；NADH1(呼吸链NADH脱氢酶，49KD亚单位家族蛋白)；DRH7(DEAD-box ATP依赖性RNA解旋酶7)；PUF1(含有未知功能域的植物蛋白)；PK1(丙酮酸激酶，推测)；DN-DTP17(表达蛋白)；CuAO1(铜胺氧化酶，推测)；FAO1(酒精氧化酶，推测)；DN-DTP9(保守假设蛋白)；DN-DTP18(表达蛋白)；SCP52(OsSCP52-推定的丝氨酸羧肽酶同系物)；和DN-DTP19(表达蛋白)。
一方面，本公开发明提供的多核苷酸编码的多肽的氨基酸序列与氨基酸序列SEQID NO:3、5、8、11、14、17、20、23,26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158中的任何一个都有至少80％(例如，81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或100％)的一致性。
“OsFBX335”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsFBX335多肽(SEQID NO:3和5)由水稻基因位点LOC_Os09g32860.1上的编码序列(CDS)(SEQ ID NO:2和4)或核苷酸序列(SEQ ID NO:1)编码，在TIGR的注释为“OsFBX335-F-box结合域蛋白，表达”。“FBX335多肽”在这里是指OsFBX335多肽及其同源物(例如，由SEQ ID NO:69编码的SEQ IDNO:70)或来自其他生物体的同源物，比如玉米(由SEQ ID NO:71编码的SEQ ID NO:72)和高粱(SEQ ID NO:73编码的SEQ ID NO:74)。
“OsNADH1”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsNADH1多肽(SEQ IDNO:8)由水稻基因位点LOC_Os07g41300.1处的编码序列(CDS)(SEQ ID NO:7)或核苷酸序列(SEQ ID NO:6)编码。在TIGR中被注释为“呼吸链NADH脱氢酶，49KD亚单位家族蛋白，表达”。“NADH1多肽”在本文中指OsNADH1多肽及其同源物(例如SEQ ID NO:75编码的SEQ ID NO:76)或来自其他生物体的同源物，例如玉米(SEQ ID NO:77编码的SEQ ID NO:78)和拟南芥(SEQ ID NO:79编码的SEQ ID NO:80)。
“OsPUF1”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsPUF1多肽(SEQ IDNO:11)由水稻基因位点LOC_Os03g19700.1处的编码序列(CDS)(SEQ ID NO:10)或核苷酸序列(SEQ ID NO:9)编码，在TIGR中被注释为“含有未知功能域蛋白的植物蛋白，表达”。“PUF1多肽”在本文中指OsPUF1多肽及其同源物(例如SEQ ID NO:81编码的SEQ ID NO:82)或来自于其它生物体的同源物，例如玉米(SEQ ID NO:83编码的SEQ ID NO:84)、高粱(SEQ ID NO:85编码的SEQ ID NO:86)、拟南芥(SEQ ID NO:87编码的SEQ ID NO:88)和大豆(SEQ ID NO:89编码的SEQ ID NO:90)。
“OsPK1”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsPK1多肽(SEQ ID NO:14)由水稻基因位点LOC_Os01g47080.1处的编码序列(CDS)(SEQ ID NO:13)或核苷酸序列(SEQ ID NO:12)编码，在TIGR中被注释为“丙酮酸激酶，推定，表达”。“PK1多肽”在本文中指OsPK1多肽及其同源物(例如SEQ ID NO:91编码的SEQ ID NO:92)或来自于其它生物体的同源物，例如玉米(SEQ ID NO:93编码的SEQ ID NO:94)、高粱(SEQ ID NO:95编码的SEQ IDNO:96)、拟南芥(SEQ ID NO:97编码的SEQ ID NO:98)和大豆(SEQ ID NO:99编码的SEQ IDNO:100)
“OsFAO1”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsFAO1多肽(SEQ IDNO:17)由水稻基因位点LOC_Os02g40840.1处的编码序列(CDS)(SEQ ID NO:16)或核苷酸序列(SEQ ID NO:15)编码，在TIGR中被注释为“酒精氧化酶，推定，表达”。“FAO1多肽”在本文中指OsFAO1多肽及其同源物(例如SEQ ID NO:101编码的SEQ ID NO:102)或来自于其它生物体的同源物，例如玉米(SEQ ID NO:103编码的SEQ ID NO:104)、高粱(SEQ ID NO:105编码的SEQ ID NO:106)、拟南芥(SEQ ID NO:107编码的SEQ ID NO:108)和大豆(SEQ ID NO:109编码的SEQ ID NO:110)
“OsDN-DTP17”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsDN-DTP17多肽(SEQ ID NO:20)由水稻基因位点LOC_Os01g19400.1处的编码序列(CDS)(SEQ ID NO:19)或核苷酸序列(SEQ ID NO:18)编码，在TIGR中被注释为“表达蛋白”。“DN-DTP17多肽”在本文中指OsDN-DTP17多肽及其同源物(例如SEQ ID NO:111编码的SEQ ID NO:112)或来自于其它生物体的同源物，例如玉米(SEQ ID NO:113编码的SEQ ID NO:114)、高粱(SEQ ID NO:115编码的SEQ ID NO:116)、拟南芥(SEQ ID NO:117编码的SEQ ID NO:118)和大豆(SEQ IDNO:119编码的SEQ ID NO:120)。
“OsCuAO1”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsCuAO1多肽(SEQ IDNO:23)由水稻基因位点LOC_Os04g39120.1处的编码序列(CDS)(SEQ ID NO:22)或核苷酸序列(SEQ ID NO:21)编码，在TIGR中被注释为“铜胺氧化酶，推定，表达”。“CuAO1多肽”在本文中指OsCuAO1多肽及其同源物(例如SEQ ID NO:121编码的SEQ ID NO:122)或来自于其它生物体的同源物，例如玉米(SEQ ID NO:123编码的SEQ ID NO:124)、高粱(SEQ ID NO:125编码的SEQ ID NO:126)。
“OsDRH7”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsDRH7多肽(SEQ IDNO:26)由水稻基因位点LOC_Os09g34910.1处的编码序列(CDS)(SEQ ID NO:25)或核苷酸序列(SEQ ID NO:24)编码，在TIGR中被注释为“DEAD-box ATP依赖性RNA解旋酶7，推测，表达”。“DRH7多肽”在本文中指OsDRH7多肽及其同源物(SEQ ID NO:127编码的SEQ ID NO:128)或来自于其它生物体的同源物，例如玉米(SEQ ID NO:129编码的SEQ ID NO:130)、高粱(SEQ ID NO:131编码的SEQ ID NO:132)、拟南芥(SEQ ID NO:133编码的SEQ ID NO:134)和大豆(SEQ ID NO:135编码的SEQ ID NO:136)
“OsDN-DTP9”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsDN-DTP9多肽(SEQ ID NO:29)由水稻基因位点LOC_Os07g45810.1处的编码序列(CDS)(SEQ ID NO:28)或核苷酸序列(SEQ ID NO:27)编码，在TIGR中被注释为“保守假设蛋白”。“DN-DTP9多肽”在本文中指OsDN-DTP9多肽及其同源物(例如SEQ ID NO:137编码的SEQ ID NO:138)或来自于其它生物体的同源物。
“OsDN-DTP18”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsDN-DTP18多肽(SEQ ID NO:32)由水稻基因位点LOC_Os09g12780.1处的编码序列(CDS)(SEQ ID NO:31)或核苷酸序列(SEQ ID NO:30)编码，在TIGR中被注释为“表达蛋白”。“DN-DTP18多肽”在本文中指OsDN-DTP18多肽及其同源物或来自于其它生物体的同源物，例如玉米(SEQ ID NO:139编码的SEQ ID NO:140)、高粱(SEQ ID NO:141编码的SEQ ID NO:142)、拟南芥(SEQ ID NO:143编码的SEQ ID NO:144)和大豆(SEQ ID NO:145编码的SEQ ID NO:146)。
“OsSCP52”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsSCP52多肽(SEQ IDNO:35)由水稻基因位点LOC_Os11g24320.1处的编码序列(CDS)(SEQ ID NO:34)或核苷酸序列(SEQ ID NO:33)编码，在TIGR中被注释为“OsSCP52-推测的丝氨酸羧肽酶同系物”。“SCP52多肽”在本文中指OsSCP52多肽及其同源物(例如SEQ ID NO:147编码的SEQ ID NO:148)或来自于其它生物体的同源物，例如玉米(SEQ ID NO:149编码的SEQ ID NO:150)、高粱(SEQ ID NO:151编码的SEQ ID NO:152)、拟南芥(SEQ ID NO:153编码的SEQ ID NO:154)和大豆(SEQ ID NO:155编码的SEQ ID NO:156)。
“OsDN-DTP19”是指一种水稻多肽，在过量表达时赋予耐旱表型。OsDN-DTP19多肽(SEQ ID NO:38)由水稻基因位点LOC_Os01g11360.1处的编码序列(CDS)(SEQ ID NO:37)或核苷酸序列(SEQ ID NO:36)编码，在TIGR中被注释为“表达蛋白”。“DN-DTP19多肽”在本文中指OsDN-DTP19多肽及其同源物(例如SEQ ID NO:157编码的SEQ ID NO:158)或来自于其它生物体的同源物。
应理解，如本领域技术人员所认知的，本发明所涵盖的不仅仅是具体的示例性序列。本领域内众所周知，核酸片段中的替代物导致在给定位点产生化学等效氨基酸，但不影响所编码多肽的功能性质。例如，氨基酸丙氨酸(疏水性氨基酸)的密码子可由编码另一疏水性较小的残基(例如甘氨酸)或疏水性较大的残基(例如缬氨酸、亮氨酸或异亮氨酸)的密码子取代。类似地，导致一个带负电的残基替换另一个残基的变化，如用天冬氨酸替换谷氨酸，或一个带正电的残基替换另一个残基，如用赖氨酸替换精氨酸，也可预期产生功能等效的产物。导致多肽分子N端和C端部分改变的核苷酸变化也不会改变多肽的活性。所提议的每一个修改都在本领域的常规技术范围内，如同确定编码产品的生物活性保留一样。
B.重组DNA构建体
还提供了含有任意本文描述的多核苷酸的重组DNA构建体。在某些实施例中，所述重组DNA构建体还包含有至少一个调控元件。在某些实施例中，所述的至少一种调控元件是一种异源调控元件。在某些实施例中，重组DNA构建体中的至少一种调控元件包含有一个启动子。在某些实施例中，所述启动子是一种异源启动子。
大量的启动子可以被用于本文中的重组DNA构建体。所述启动子能够根据期望结果进行选择，并且可以包括组成型、组织特异性、诱导型或用于在宿主生物体中表达的其他启动子。
“组成型”启动子是一种能够在大多数环境中有活性的启动子。组成型启动子包括，例如，Rsyn7启动子的核心启动子或在WO 99/43838和美国专利号6072050中公开的其他组成型启动子；CaMV35S核心启动子(Odell等,(1985)Nature,313:810-812)；水稻肌动蛋白(McElroy等人,(1990)植物细胞,2:163-171)；泛素(Christensen等，(1989)，PlantMol.Biol.，12:619-632和Christensen等人，(1992)Plant Mol.Biol.，18:675-689)；pEMU(Last等,(1991)Theor.Appl.Genet.,81:581-588)；MAS(Velten等,(1984)EMBO J.,3:2723-2730)；ALS启动子(美国专利号5659026)等。其他组成型启动子包括，例如，美国专利号5608149、5608144、5604121、5569597、5466785、5399680、5268463、5608142和6177611。
组织特异性或发育调控启动子是一种DNA序列，能够选择性调节植物细胞/组织中DNA序列的表达。例如在这些细胞/组织中至关重要的雄穗发育、种子形成或两者都有的生长发育时期，通常将DNA序列的表达限制在植物中所需的发育期(如雄穗发育或种子成熟)。可在本发明的方法中使用会引起所需时间和空间表达的任何可识别的启动子。
许多叶片优选启动子在本领域内是已知的(Yamamoto等,(1997)Plant J.,12(2):255-265；Kwon等,(1994)Plant Physiol.,105:357-367；Yamamoto等,(1994)Plant CellPhysiol.,35(5):773-778；Gotor等,(1993)Plant J.,3:509-518；Orozco等,(1993)PlantMol.Biol.,23(6):1129-1138；and Matsuoka等,(1993)Proc.Natl.Acad.Sci.USA,90(20):9586-9590)。
可能用于本发明的种子或胚胎特异性的启动子，包括大豆Kunitz胰蛋白酶抑制子(Kti3，Jofuku和Goldberg.，(1989)Plant Cell,1:1079-1093)、豌豆球蛋白convicilin、vicilin和legumin(豌豆子叶)(Rerie，W.G.，等(1991)Mol.Genet.,259:149-157；Newbigin,E.J.等(1990)Planta,180:461-470；Higgins,T.J.V.等人(1988年)Plant.Mol.Biol.,11:683-695)、玉米醇溶蛋白(玉米胚乳)(Schemthaner，J.P.等,(1988)EMBO J.,7:1249-1255)，菜豆子叶(Segupta-Gopalan,C.,等,(1985)Proc.Natl.Acad.Sci.,82:3320-3324)、植物血凝素(豆子叶)(Voelker,T.等(1987)EMBOJ.6:3571-3577)，B-伴甘氨酸和甘氨酸(豆子叶)(Chen，Z-L，等(1988)EMBO J.7:297-302)，谷蛋白(水稻胚乳)、大麦醇溶蛋白(大麦胚乳)(Marris，C.，等(1988)Plant Mol.Biol.,10:359-366)、麦谷蛋白和醇溶蛋白(小麦胚乳)(Colot，V.，等,(1987)EMBO J.,6:3559-3564)。与嵌合基因结构中异源编码区可操作连接的种子特异基因的启动子在转基因植物中保持其时空表达模式。这些例子包括在拟南芥和甘蓝型油菜种子中表达脑啡肽的拟南芥2S种子贮藏蛋白基因启动子(Vanderkerckhove等(1989)Bio/Technology 7:L929-932)、表达荧光素酶的大豆凝集素和大豆β-豆素启动子(Riggs等(1989)Plant Sci.,63:47-57)，以及表达氯霉素乙酰转移酶的小麦谷蛋白启动子(Colot等人(1987)EMBO J 6:3559-3564)。
可诱导启动子选择性表达可操作连接的DNA序列，以响应内源性或外源性刺激的存在，例如通过化合物(化学诱导物)或响应环境、激素、化学和/或发育信号。可诱导或调节的启动子包括，例如，受光、热、应激、洪水或干旱、植物激素、创伤或诸如乙醇、茉莉酸、水杨酸或安全剂等化学物质调节的启动子。
还考虑合成启动子，其包括一种或多种异源调节元件的组合。
本发明的重组DNA构建体的启动子可以是本领域已知的任何类型或类别的启动子，从而可以使用多个启动子中的任何一个来表达本文公开的各种多核苷酸序列，包括感兴趣的多核苷酸序列的天然启动子。可基于期望结果选择用于本发明重组DNA构建体中的启动子。
本发明的重组DNA构建体还可包括其他调控元件，包括但不限于翻译先导序列、内含子和聚腺苷酸化识别序列。在某些实施例中，重组DNA构建体进一步包含增强子或沉默子。
可将内含子序列添加到5'-非翻译区、蛋白质编码区或3'-非翻译区，以增加积累在细胞质中的成熟信息量。在植物和动物表达结构的转录单元中加入可剪接的内含子已被证明可以在mRNA和蛋白质水平上将基因表达提高1000倍(Buchman和Berg.,(1988)Mol.Cell Biol.,8:4395-4405；Callis等人(1987)Genes Dev.,1:1183-1200)。
C.植物和植物细胞
提供的植物、植物细胞、植物部分、种子和籽粒，在其基因组中含有任意本文描述的重组DNA构建体，因而所述植物、植物细胞、部分植物、种子和/或籽粒具有增加的编码多肽的表达。
还提供了在其基因位点上包含有一种引入的基因修饰的植物、植物细胞、部分植物、种子和籽粒，使其编码的多肽的氨基酸序列与SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158这些氨基酸序列相比较时有至少80％的一致性。在某些实施例中，该基因修饰增加了编码多肽的活性。在某些实施例中，该基因修饰增加了编码多肽的表达水平。在某些实施例中，该基因修饰同时增加了编码多肽的表达水平和活性。
所述植物可以是单子叶植物或双子叶植物，例如，水稻或玉米或大豆植物，例如玉米杂交植株或玉米自交植株。所述植物还可以是向日葵、高粱、油菜、小麦、苜蓿、棉花、大麦、小米、甘蔗或柳枝稷。
在某些实施例中，所述植物与对照植物相比较时表现出增加的耐旱性。在某些实施例中，所述植物与对照植物相比较时，表现出至少一种农艺性状的改变。
本领域内技术人员熟悉模拟干旱条件和评估遭受模拟或自然发生干旱条件的植物耐旱性的方法。例如，可以通过在一段时间内给植物提供低于正常需要的水分或没有水分来模拟干旱条件，也可以通过寻找生理和/或物理条件的差异来评估耐旱性，包括(但不限于)活力、生长、大小或根长，尤其是，叶颜色或叶面积大小。评估耐旱性的其他技术包括测量叶绿素荧光、光合速率和气体交换速率。
D.其它目的性状的堆叠
在某些实施例中，本文中发明的多核苷酸被设计成一种分子堆叠。这样，本文所述的多种宿主细胞、植物、植物细胞、植物部分、种子和/或籽粒能进一步包含一个或多个期望的性状。在某些实施例中，这些宿主细胞、植物、部分植物、种子和/或籽粒可以任何多核苷酸序列的组合形式进行堆叠，以产生具有期望性状组合的植物。如本文所用，术语“堆叠”是指在同一植物或有机体中具有多种性状。例如，“性状堆叠”可以包含一种序列在物理上互相毗邻的分子堆叠。在本文中，一种性状是指来自于特定序列或序列组的表型。在一个实施例中，分子堆叠包括至少一个能赋予草甘膦耐性的多核苷酸。这种赋予草甘膦耐性的多核苷酸在领域内是已知的。
在某些实施例中，分子堆叠包含至少一个赋予草甘膦耐性的多核苷酸和至少一个赋予第二除草剂耐性的附加的多核苷酸。
在某些实施例中，含有本发明的多核苷酸序列的植物、植物细胞、种子和/或籽粒可以被和一种或多种赋予以下耐性的序列进行堆叠，例如：ALS抑制剂；HPPD抑制剂；2,4-D；其他苯氧基生长素除草剂；芳氧基苯氧基丙酸除草剂；麦草畏；草铵膦除草剂；针对protox酶的除草剂(也称为“protox抑制剂”)。
含有本发明的多核苷酸序列的植物、植物细胞、植物部分、种子和/或籽粒还能与至少一个其它性状相结合以产生进一步包含有多种期望的性状组合的植物。例如，具有本发明多核苷酸序列的植物、植物细胞、植物部分、种子和/或籽粒可与编码具有杀虫和/或杀虫活性的多肽的多核苷酸进行堆叠，或植物、植物细胞、植物部分、种子和/或具有本发明多核苷酸序列的籽粒可与植物抗病基因结合。
这些堆叠组合可以使用任何方法来创建，包括但不限于，使用任何常规育种方法，或基因转化方法。如果所述序列通过对植物进行基因转化而堆叠的，该目的多核苷酸序列能够在任何时间以任何顺序进行结合。这些性状可以在共转化方案中与任何转化盒组合提供的相关多核苷酸同时引入。例如，如果两个序列将被引入，这两个序列可以被包含在单独的转化盒(trans)或包含在相同的转化盒(cis)中。该序列的表达受到相同启动子或不同启动子的驱动。在某些情况下，也可以期望引入一个转化盒来抑制目标多核苷酸的表达。可以与任何其它抑制盒或过表达盒的组合进行结合，以产生植物中期望性状的组合。需要进一步认识到，多核苷酸序列能使用位点特异性重组方法在所需的基因位点上进行堆叠。参见，例如，WO99/25821、WO99/25854、WO99/25840、WO99/25855和WO99/25853，以上均通过引用并入本文。
方法：
提供了一种增加植物耐旱性和/或增加籽粒产量的方法，包括增加至少一个编码多肽的多核苷酸的表达，该多肽的氨基酸序列与SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70,72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114,116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150,152、154、156或158至少有80％(例如，80％、81％、82％、83％、84％,85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或100％)的一致性。
在某些实施例中，所述方法包括：(a)在一个可再生植物细胞中表达一个重组DNA构建体，该重组DNA构建体含有一个调控元件可操作地连接一个编码多肽的多核苷酸；和(b)再生所述植物，其中该植物在其基因组中包含有所述重组DNA构建体。在某些实施例中，所述调控元件是一个异源启动子。
在某些实施例中，所述方法包括：(a)在一个可再生植物细胞的编码多肽的基因组位点上引入一个靶向基因修饰；和(b)再生所述植物，其中植物中所述编码多肽的表达水平和/或活性是增加的。在某些实施例中，所述靶向基因修饰是使用基因修饰技术被引入的，包括：多核苷酸引导核酸内切酶、CRISPR-Cas核酸内切酶、碱基编辑脱氨酶、锌指核酸酶、转录激活物样效应器核酸酶(TALEN)、工程化位点特异性巨核酸酶或Argonaute。在某些实施例中，所述靶向基因修饰存在于基因位点的(a)编码区；(b)非编码区；(c)调控序列；(d)非翻译区；或(e)任何(a)-(d)的组合。所述多肽包含的氨基酸序列与SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88,90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126,128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158这些氨基酸序列相比较有至少80％的一致性。
在某些实施例中，所述DNA修饰是基因位点上的一个或多个核苷酸，甚至是相邻的。例如，一个表达调节元件(EME)插入可操作连接的基因中，如PCT/US2018/025446所描述的。在某些实施例中，所述靶向DNA修饰可以用另一种领域内已知的具有高表达的启动子替换内源性多肽启动子。在某些实施例中，所述靶向DNA修饰可以插入一个领域内已知的具有高表达的启动子到5’UTR，以使内源性多肽的表达受到插入的启动子的控制。在某些实施例中，所述DNA修饰是一种优化Kozak序列以增加表达的修饰。在某些实施例中，所述DNA修饰是一种位点上的多核苷酸修饰或SNP，可以调节表达蛋白的稳定性。
使用本发明的方法的植物可以是文中描述的任何种类的植物。在某些实施例中，这些植物是玉米、大豆或水稻。多种方法能被用于向植物、植物部分、植物细胞、种子和/或籽粒引入一个期望的序列。“引入”在这里是指把本发明的多核苷酸或产生的多肽提供给植物、植物细胞、种子和/或籽粒，使该序列获得进入植物细胞内部的途径。本公开发明的方法不依赖于特定的方法将序列引入植物、植物细胞、种子和/或籽粒，只是单纯的让所述多核苷酸或多肽能够进入植物的至少一个细胞内部。
转化方法是引入多肽或多核苷酸序列到植物中的方法，可以根据转化目标植物或植物细胞(即双子叶植物或单子叶植物)类型的不同而异。合适的引入多肽和多核苷酸到植物细胞的方法微量注射(Crossway等(1986)Biotechniques，4:320-334)、电穿孔(Riggs等(1986)Proc.Natl.Acad.Sci.USA，83:5602-5606)、农杆菌介导的转化(美国专利号5563055和美国专利号5981840)、直接基因转移(Paszkowski等(1984)EMBO J.3:2717-2722)，和弹道粒子加速(例如，见美国专利号4945050、美国专利号5879918、美国专利号5886244和美国专利号5932782；Tomes等人(1995)，in Plant Cell,Tissue,and Organ Culture:Fundamental Methods,编著者Gamborg和Phillips(柏林Springer Verlag)；McCabe等人(1988)Biotechnology 6:923-926)、和Lec1转化(WO 00/28058)。另见Weissinger等人(1988年Ann.Rev.Genet.，22:421-477；Sanford等人(1987)Particulate Science andTechnology，5:27-37(洋葱)；Christou等人(1988年)Plant Physiol.，87:671-674(大豆)；McCabe等人(1988)Bio/Technology 6:923-926(大豆)；Finer和McMullen(1991)In VitroCell Dev.Biol.，27P:175-182(大豆)；Singh等人(1998)Theor.Appl.Genet.，96:319-324(大豆)；Datta等人(1990)Biotechnology 8:736-740(水稻)；Klein等人(1988年)Proc.Natl.Acad.Sci.USA，85:4305-4309(玉米)；Klein等人(1988)Biotechnology 6:559-563(玉米)；美国专利号5240855、5322783及5324646；Klein等人(1988)Plant Physiol.，91:440-444(玉米)；Fromm等人(1990)Biotechnology 8:833-839(玉米)；Hooykaas VanSlogteren等人(1984)Nature(London)311:763-764；美国专利号5736369(谷物)；Bytebier等人(1987年)Proc.Natl.Acad.Sci.USA，84:5345-5349(百合科)；De Wet等(1985)在胚珠组织的实验操作中，编著者Chapman等(纽约朗文)，第197-209页(花粉)；Kaeppler等(1990)Plant Cell Reports 9:415-418和Kaeppler等(1992)Theor.Appl.Genet.，84:560-566(晶须介导的转化)；D'Halluin等(1992)Plant Cell，4:1495-1505(电穿孔)；Li等(1993)PlantCell Reports，12:250-255和Christou和Ford(1995)Annals of Botany 75:407-413(水稻)；Osjoda等人(1996)《Nature Biotechnology14:745-750(玉米和根癌农杆菌)；所有这些都通过引用并入本文。
在另一些实施例中，本发明的多核苷酸可以通过病毒或病毒核酸接触被引入植物中。通常，这些方法涉及到在DNA或RNA分子内结合本发明的核苷酸结构。需要认识到，本发明的多核苷酸序列最初可作为病毒多蛋白的一部分进行人工合成，随后可以在体内或体外通过蛋白质水解处理以产生所需的重组蛋白。进一步，还应认识到本文公开的启动子也包括用于病毒RNA聚合酶转录的启动子。向植物引入多核苷酸并在其中表达编码蛋白的方法，涉及到病毒DNA或RNA分子，这在本领域内是已知的。例如，参见美国专利5889191、5889190、5866785、5589367、5316931和Porta等人,(1996)Molecular Biotechnology，5:209-221。这些文本通过引用并入本文。
这些转化的细胞可以按照常规方法生长成为植物。参见McCormick等,(1986)Plant Cell Reports,5:81-84。然后可以生长这些植物，并用相同的转化品种或不同的品种授粉，得到的后代具有所需表型特征的组成型表达。可以种植两代或更多代以确定所需表型特征的表达能够稳定保持和遗传，然后收获的种子也能够确保获得所需的表型特征。以这种方式，本发明提供的转化种子(也称为“转基因种子”)，其具有本文公开的多核苷酸，作为表达盒的一部分，稳定地并入其基因组中。
通过植物转化技术获得的前文讨论的这些转化植物细胞，可以培养再生成整个拥有转化表型(即，发明的多核苷酸)的植株，并获得所需的表型，例如增加的产量。关于玉米的转化和再生，参见Gordon-Kamm等,(1990)The Plant Cell,2:603-618。
多种方法可以用于向基因位点引入基因修饰，以在植物、植物部分、植物细胞、种子和/或籽粒中编码本文的多肽。在某些实施例中，所述靶向DNA修饰通过选自以下的基因修饰技术：多核苷酸引导的核酸内切酶、CRISPR-Cas核酸内切酶、碱基编辑脱氨酶、锌指核酸酶、转录激活物样效应器核酸酶(TALEN)、工程化位点特异性巨核酸酶或Argonaute。
在一些实施例中，所述基因修饰可以通过在基因组中靠近所需改变的特定位置诱导双链断裂(DSB)或单链断裂来推进。双链断裂(DSB)的诱导能使用任何双链断裂诱导剂进行，包括但不限于，TALENs、巨核酸酶、锌指核酸酶、Cas9-gRNA系统(基于细菌CRISPR-Cas系统)、引导cpf1核酸内切酶系统，等等。在一些实施例中，双链断裂的引入可以与多核苷酸修饰模板的引入相结合。
多核苷酸修饰模板可以通过任何本领域内已知的方法被引入，比如，但不限于，瞬时导入法、转染、电穿孔、微量注射、颗粒介导的递送、局部递送、晶须介导的递送、通过细胞穿透肽的递送或介孔二氧化硅纳米颗粒(MSN)介导的直接递送。
所述多核苷酸修饰模板能作为一个单链多核苷酸分子、一个双链多核苷酸分子或作为一个环状DNA(载体DNA)的一部分被引入细胞。该多核苷酸修饰模板还能被束缚在向导RNA和/或Cas内切核酸酶上。
“修饰核苷酸”或“编辑核苷酸”是指与它的不含有修饰核苷酸序列相比较，包含至少一个改变的目的核苷酸序列。这种“改变”包括，例如：(i)至少一个核苷酸的替换，(ii)至少一个核苷酸的删除，(iii)至少一个核苷酸的插入，或(iv)任何(i)-(iii)的组合。
术语“多核苷酸修饰模板”包括一个与被编辑的核苷酸序列相比较时含有至少一个核苷酸修饰的多核苷酸。一个核苷酸修饰能被至少一个核苷酸替代、添加或删除。通常，该多核苷酸修饰模板能进一步包含同源核苷酸序列侧翼至少一个核苷酸修饰，其中该侧翼异源核苷酸序列提供足够的同源物到所需的核苷酸序列用于编辑。
编辑结合双链断裂(DSB)和修饰模板的基因组序列的过程通常包括：提供一个宿主细胞，一个双链断裂(DSB)诱导剂，或一个编码双链断裂(DSB)诱导剂的核酸，以识别染色体序列中的靶向序列并能够诱导基因组序列中的双链断裂(DSB)，以及与被编辑的核苷酸序列相比较，含有至少一个核苷酸改变的至少一个多核苷酸修饰模板。该多核苷酸修饰模板能进一步包含至少一个核苷酸改变的核苷酸序列的侧翼序列，其中侧翼序列与双链断裂(DSB)侧翼的染色体区域基本同源。
核酸内切酶可以通过任何本领域内已知的方法被提供给细胞，例如，但不限于，瞬时导入法、转染、微量注射和/或局部应用或通过重组结构间接应用。核酸内切酶能作为蛋白或作为向导多核苷酸复合物直接提供给细胞或间接通过重组构建体提供。核酸内切酶通过使用本领域内已知的任何方法能短暂地被引入细胞，或能结合到宿主细胞的基因组。在CRISPR-Cas系统的情况下，如2016年5月12日出版的WO2016073433所述，细胞穿透肽(CPP)可促进内切酶和/或引导多核苷酸进入细胞。
除了通过双链断裂技术进行修饰外，还可以使用碱基编辑技术实现一个或多个碱基的无双链断裂修饰，例如，Gaudelli等人，(2017)Programmable base editing of A*Tto G*C in genomic DNA without DNA cleavage,Nature,551(7681)：464-471；Komor等人，(2016)Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage,Nature,533(7603)：420-425。
这些融合包括dCas9或Cas9切口酶以及一个合适的脱氨酶，并且它们可以将，例如胞嘧啶转化为尿嘧啶，而不会导致目标DNA的双链断裂。尿嘧啶然后通过DNA复制或修复转化为胸腺嘧啶。改进的具有靶向灵活性和特异性的碱基编辑器用于编辑内源基因座以产生靶向变异并提高粮食产量。类似地，腺嘌呤碱基编辑器使腺嘌呤转变为肌苷，然后通过修复或复制转化为鸟嘌呤。因此，使用适当的站点特定的基础编辑器，在一个或多个位置进行有针对性的碱基改变，即C·G到T·A转换和A·T到G·C转换。
在一个实施例中，碱基编辑是一种能够直接将目标基因组位点上的一个碱基对转变为另一个，且无需双链断裂(DSB)、同源定向修复(HDR)过程或外部供体DNA模板的基因编辑方法。在一个实施例中，碱基编辑包括(i)一种催化受损的CRISPR–Cas9突变体，其突变使其一个核酸酶结构域不能产生DSB；(ii)单链特异性胞苷/腺嘌呤脱氨酶，在Cas9产生的单链DNA泡中的适当核苷酸窗口内将C转化为U或A转化为G；(iii)尿嘧啶糖基化酶抑制剂(UGI)，阻碍尿嘧啶切除和下游过程，降低碱基编辑效率和产品纯度；(iv)尼克斯酶活性，以切割未编辑的DNA链，然后进行细胞DNA修复过程以替换含G的DNA链。
如本文所用，“基因组区域”是细胞基因组中存在于靶位点任一侧的染色体片段，或者也包括靶位点的一部分。所述基因组区域可以包括至少5-10、5-15、5-20、5-25、5-30、5-35、5-40、5-45、5-50、5-55、5-60、5-65、5-70、5-75、5-80、5-85、5-90、5-95、5-100、5-200、5-300、5-400、5-500、5-600、5-700、5-800、5-900、5-1000、5-1100、5-1200、5-1300、5-1400、5-1500、5-1600、5-1700、5-1800、5-1900、5-2000、5-2100、5-2200、5-2300、5-2400、5-2500、5-2600、5-2700、5-2800.5-2900、5-3000、5-3100或更多碱基，使得基因组区域具有足够的同源性以与相应同源区域进行同源重组。
TAL效应核酸酶(TALEN)是一类序列特异性核酸酶，可用于在植物或其他生物体基因组中的特定目标序列上进行双链断裂(Miller等,(2011)Nature Biotechnology,29:143–148)。
核酸内切酶是在多核苷酸链内切割磷酸二酯键的酶。核酸内切酶包括限制性内切酶，其在特定位点切割DNA而不损伤碱基；以及巨核酸酶，也称为归巢内切酶(HEASE)，其与限制性内切酶类似，在特定识别位点结合并切割，但是巨核酸酶的识别位点通常较长，大约18bp或更长(专利申请号PCT/US12/30061，于2012年3月22日提交)。
根据保守的序列基序，巨核酸酶被分为四个家族，分别是LAGLIDADG、GIY-YIG、H-N-H和His Cys-box家族。这些基序参与金属离子的配位和磷酸二酯键的水解。HEASE以其长识别位点和耐受DNA底物中的某些序列多态性而著名。巨核酸酶的命名规则与其他限制性内切酶的命名规则相似。对于分别由独立ORF、内含子和内含子编码的酶，巨核酸酶也以前缀F-、I-或PI-为特征。重组过程中的一个步骤涉及多核苷酸在识别位点或其附近的切割。
解理活性可用于产生双链断裂。关于位点特异性重组酶及其识别位点的综述，见Sauer(1994)Curr Op Biotechnol 5:521-7；和Sadowski(1993)FASEB 7:760-7。在一些例子中，重组酶来自整合酶或分解酶家族。
锌指核酸酶(ZFNs)是由锌指DNA结合域和双链断裂诱导剂域组成的工程化双链断裂诱导剂。识别位点特异性由锌指结构域赋予，锌指结构域通常包括两个、三个或四个锌指，例如具有C2H2结构，然而其他锌指结构是已知的，并且已经改变基因的结构。锌指结构域适于设计特异性结合一个选定的多核苷酸识别序列的多肽。锌指核酸酶(ZFN)包括连接到非特异性核酸内切酶结构域的工程DNA结合锌指结构域，例如来自IIs型核酸内切酶(例如FokI)的核酸酶结构域。其他功能可以融合到锌指结合域，包括转录激活域、转录抑制域和甲基化酶。在一些实例中，切割活性需要核酸酶结构域的二聚化。每个锌指识别目标DNA中的三个连续碱基对。例如，一个3指结构域识别9个相邻的需要核酸酶二聚化核的苷酸的序列，两组锌指三联体用于结合18个核苷酸的识别序列。
使用双键断裂(DSB)诱导剂(如Cas9-gRNA复合物)进行基因组编辑已有叙述，例如在2015年3月19日发布的美国专利申请US 2015-0082478 A1、2015年2月26日发布的WO2015/026886A1、2016年1月14日发布的WO2016007347和2016年2月18日发布的WO201625131中，所有这些都通过引用并入本文。
实例
以下是本发明某些方面的具体实施例的示例。这些实施例仅用于说明目的，并不打算以任何方式限制本发明的范围。
实例1
耐旱基因的克隆和载体构建
使用含有四个来自花椰菜花叶病毒35S(CaMV 35S)启动子的多聚增强子元件的双元载体，并从四个粳稻品种(中花11、超优1号、台中65和日本晴)开发水稻激活标签群体，按照Lin和Zhang((2005)Plant Cell Rep.23:540-547)所述，通过农杆菌介导的方法转化。建立转基因株系，收获转基因种子，形成水稻激活标签群体。
耐旱标签株系(ATL)经重复的田间试验进行验证，并确定了T-DNA的插入位点。通过测序法(Zastrow-Hayes G.M.等,(2015)The Plant Genome,8:1-15)确定了T-DNA在ATL株系中的插入位点。克隆该T-DNA左右两侧附近的基因，并通过田间筛选重现该功能基因。此处仅显示了重现功能的基因。依据表2所示的LOC ID和这些基因相应的基因序列，设计引物克隆水稻抗旱基因OsFBX335(使用SEQ ID NO:39和40)、OsNADH1(使用SEQ ID NO:41和42)、OsPUF1(使用SEQ ID NO:43和44)、OsPK1(使用SEQ ID NO:45和46)、OsFAO1(使用SEQID NO:47和48)、OsDN-DTP17(使用SEQ ID NO:49和50)、OsCuAO1(使用SEQ ID NO:51和52)、OsDRH7(使用SEQ ID NO:53和54)、OsDN-DTP9(使用SEQ ID NO:55和56)、OsDN-DTP18(使用SEQ ID NO:57和58)、OsSCP52(使用SEQ ID NO:59和60)、和OsDN-DTP19(使用SEQ ID NO:61和62)。
表2.水稻基因名称、基因ID(来自TIGR)和构建体ID
Figure BDA0003493940310000191
Figure BDA0003493940310000201
使用柱试剂盒在琼胶凝脂电泳后提取的PCR扩增产物，然后与TA克隆载体连接。通过测序确认了这些构建体内的序列和方向。每个基因都被克隆到一个植物双元载体中。
实例2
转基因水稻株系的转化和基因表达分析
如Lin和Zhang((2005)Plant Cell Rep.23:540-547)所述，通过农杆菌介导的转化，使用实施例1中制备的载体或空载体(DP0158)转化中花11(Oryza sativa L.)。将转化实验室产生的转基因苗(T0)移栽到田间获得T1种子。筛选T1和随后的T2种子以确认转化，并在以下性状筛选中使用阳性鉴定的转基因种子。
通过RT-PCR测定转基因水稻叶片中的基因表达水平。设计引物对过表达的转基因水稻中的OsFBX335(用SEQ ID NO:63和64)、OsDRH7(用SEQ ID NOs:65和66)、以及OsPK1(用SEQ ID NOs:67和68)基因进行RT-PCR。将ZH11-TC(组织培养ZH11水稻)中的表达水平设置为1.00，并将DP0955、DP0933和DP1004转基因水稻植株中的表达水平与ZH11-TC进行比较。基因表达根据EF-1αmRNA水平进行标准化，基因表达分析结果见下表3。
表3.转基因水稻植株中相关表达水平的增长倍数
基因名称 构建体ID 相关表达水平的增长倍数
OsFBX335 DP0955 从97.14到93892.60
OsDRH7 DP0933 从2.72到324.88
OsPK1 DP1004 从10.23到160.62
实例3
转基因水稻植物的性状
对来自于实例2的转基因水稻株系和ZH11-TC以及DP0158进行耐旱性测试。将来自实施例2的植物的T2种子在32℃下用800ppm多菌灵杀菌8小时，然后洗涤3-5次，在32℃下用水浸泡16小时，之后在35-37℃培养箱中催芽18小时。发芽种子用于以下耐旱性试验。
耐旱性试验-将发芽种子种植在苗床中。在三叶期，将幼苗移栽到试验田，每个转基因株系4个重复，每个重复种植10株，4个重复种植在同一田块中。ZH11-TC和DP0158幼苗位于同一田块的转基因株系附近，并作为对照进行统计分析。水稻植株按常规使用杀虫剂和化肥进行管理。在穗分化期停止浇水，以便根据天气条件(温度和湿度)在开花期给予干旱胁迫。使用TDR30(Spectrum Technologies，Inc.)在每个区块约10个地点每4天测量一次土壤含水量。在实验过程中观察并记录植物表型。表型包括抽穗期、卷叶程度、干旱敏感性和耐旱性。中午特别注意卷叶程度。在生长季结束时，从每行中间收获每一转基因株系的6株代表性植株，并测量每株的粮食产量。采用混合线性模型对粮食产量数据进行统计分析。
表4提供了这些研究的结果，其中并提供了每种载体的转基因株系的组合数据。
表4.转基因水稻植株的农艺性状
Figure BDA0003493940310000211
Figure BDA0003493940310000221
DP0955-转基因水稻植株，两年内在海南田间进行了两次试验。结果一致表明，与对照相比，DP0955转基因水稻在田间干旱条件下的平均单株产量增加。如表4所示，在海南田间测试了12个株系。与ZH11-TC和DP0158对照相比，12个株系中的10个株系的产量显著提高(P<0.1)。这12个株系的平均单株产量分别比对照ZH11-TC和DP0158高39％和68％。这些数据表明OsFBX335是一个水稻耐旱基因。
DP0952-转基因水稻植物两年内在海南田间进行了两次试验。结果一致表明，与对照相比，DP0952转基因水稻在田间干旱条件下的平均单株产量增加。如表4所示，共测试了12个株系，6个株系的产量显著高于DP0158对照(P<0.1)；5个株系的产量显著高于ZH11-TC对照(P<0.1)。这12个株系的单株平均产量分别比对照ZH11-TC和DP0158高38％和51％。这些数据表明OsNADH1是水稻耐旱基因。
DP1091-转基因水稻植株两年内在海南田间进行了两次试验。结果一致表明，与对照相比，DP1091转基因水稻在田间干旱条件下的平均单株产量增加。如表4所示，共测试了12个株系，5个株系的产量显著高于DP0158对照(P<0.1)；4个株系的产量显著高于ZH11-TC对照(P<0.1)。这12个株系的单株平均产量分别比对照ZH11-TC和DP0158高31％和41％。这些数据表明OsPUF1是水稻耐旱基因。
DP1004-转基因水稻植株两年内分别在海南和宁夏田间进行了两次试验。结果一致表明，与对照相比，DP1004转基因水稻在田间干旱条件下的平均单株产量增加。如表4所示，共测试了12个株系，3个株系在海南田间表现出良好的结实率。10个株系的产量显著高于DP0158对照(P<0.1)；6个株系的产量显著高于ZH11-TC对照(P<0.1)。这12个株系的单株平均产量分别比对照ZH11-TC和DP0158高34％和80％。这些数据表明与两个对照相比，OsPK1转基因水稻植株具有更高的耐旱性。
DP2185-转基因水稻植株两年内在海南田间进行了两次试验。结果一致表明，与对照相比，DP2185转基因水稻在田间干旱条件下的平均单株产量增加。如表4所示，共测试了12个株系，6个株系在海南田间表现出良好的结实率。8个株系的产量显著高于DP0158对照(P<0.1)；7个株系的产量显著高于ZH11-TC对照(P<0.1)。这12个株系的单株平均产量分别比对照ZH11-TC和DP0158高49％和26％。这些数据表明与两个对照相比，OsFAO1转基因水稻植株具有更高的耐旱性。
DP0965-转基因水稻植株两年内在海南田间进行了两次试验。结果一致表明，与对照相比，DP0965转基因水稻在田间干旱条件下的平均单株产量增加。如表4所示，共测试了8个株系，2个株系在海南田间表现出良好的结实率。7个株系的产量显著高于DP0158对照(P<0.1)；5个株系的产量显著高于ZH11-TC对照(P<0.1)。这8个株系的单株平均产量分别比对照ZH11-TC和DP0158高31％和48％。这些数据表明与两个对照相比，OsDN-DTP17转基因水稻植株具有更高的耐旱性。
DP1385-转基因水稻植株两年内分别在海南和宁夏田间进行了两次试验。结果一致表明，与对照相比，DP1385转基因水稻在田间干旱条件下的平均单株产量增加。如表4所示，共测试了8个株系，4个株系在海南田间表现出良好的结实率。4个株系的产量显著高于DP0158对照(P<0.1)；2个株系的产量显著高于ZH11-TC对照(P<0.1)。这8个株系的单株平均产量分别比对照ZH11-TC和DP0158高30％和34％。这些数据表明与两个对照相比，OsCuAO1转基因水稻植株具有更高的耐旱性。
DP0933-转基因水稻植株两年内分别在海南和宁夏田间进行了两次试验。结果一致表明，与对照相比，DP0933转基因水稻在田间干旱条件下的平均单株产量增加。如表4所示，共测试了8个株系，4个株系在宁夏田间表现出良好的结实率。4个株系的产量显著高于DP0158对照(P<0.1)；3个株系的产量显著高于ZH11-TC对照(P<0.1)。这8个株系的单株平均产量分别比对照ZH11-TC和DP0158高57％和77％。这些数据表明与两个对照相比，OsDRH7转基因水稻植株具有更高的耐旱性。
DP2311-转基因水稻植株两年内分别在海南和宁夏田间进行了两次试验。结果一致表明，与对照相比，DP2311转基因水稻在田间干旱条件下的平均单株产量增加。如表4所示，共测试了8个株系，2个株系在海南田间表现出良好的结实率。6个株系的产量显著高于DP0158对照(P<0.1)；6个株系的产量显著高于ZH11-TC对照(P<0.1)。这8个株系的单株平均产量分别比对照ZH11-TC和DP0158高46％和35％。这些数据表明与两个对照相比，OsDN-DTP9转基因水稻植株具有更高的耐旱性。
DP2387-转基因水稻植株两年内分别在海南和宁夏田间进行了两次试验。结果一致表明，与对照相比，DP2387转基因水稻在田间干旱条件下的平均单株产量增加。如表4所示，共测试了8个株系，5个株系的产量显著高于DP0158对照和ZH11-TC对照(P<0.1)。这8个株系的单株平均产量分别比对照ZH11-TC和DP0158高79％和76％。这些数据表明与两个对照相比，OsDN-DTP18转基因水稻植株具有更高的耐旱性。
DP0255-转基因水稻植株两年内在海南田间进行了两次试验。结果一致表明，与对照相比，DP0255转基因水稻在田间干旱条件下的平均单株产量增加。如表4所示，共测试了8个株系，5个株系在海南田间表现出良好的结实率。4个株系的产量显著高于DP0158对照和ZH11-TC对照(P<0.1)。这8个株系的单株平均产量分别比对照ZH11-TC和DP0158高49％和39％。这些数据表明与两个对照相比，OsSCP52转基因水稻植株具有更高的耐旱性。
DP1055-转基因水稻植株两年内分别在海南和宁夏田间进行了两次试验。结果一致表明，与对照相比，DP1055转基因水稻在田间干旱条件下的平均单株产量增加。如表4所示，共测试了12个株系，2个株系在海南田间表现出良好的结实率。8个株系的产量显著高于DP0158对照，6个株系的产量显著高于ZH11-TC对照(P<0.1)。这12个株系的单株平均产量分别比对照ZH11-TC和DP0158高33％和46％。这些数据表明与两个对照相比，OsDN-DTP19转基因水稻植株具有更高的耐旱性。
综上所述，这些结果表明，在田间干旱胁迫条件下，与对照植株相比，OsFBX335、OsNADH1、OsPUF1、OsPK1、OsFAO1、OsDN-DTP17、OsCuAO1、OsDRH7、OsDN-DTP9、OsDN-DTP18、OsSCP52和OsDN-DTP19转基因水稻植株具有增加的耐旱性。
实例4
水稻耐旱基因在玉米中的转化和评价
玉米植株将通过编码本文所述多肽的一种多核苷酸或来自玉米、拟南芥或其他物种的相应同源物转化。该基因在玉米转化载体中的表达可由组成型启动子控制，例如玉米泛素启动子(Christensen等,(1989)Plant Mol.Biol.,12:619-632和Christensen等,(1992)Plant Mol.Biol.18:675-689)或在另一启动子的控制下，如应激反应启动子或组织优选启动子。基本上如国际专利WO 2009/006276所述，可通过粒子轰击将重组DNA构建体引入玉米细胞。或者，如Zhao等人在Meth.Mol.Biol.,318:315-323(2006)和Zhao等在Mol.Breed.,8:323-333(2001)中、以及1999年11月9日发布的美国专利号5981840中所述，玉米植株可以通过农杆菌介导的转化用重组DNA构建体转化。
再生植株的后代，如T1植株，可能受到基于土壤的干旱胁迫。通过图像分析，可以在干旱胁迫之前和期间多次测量植物的面积、体积、生长速率和颜色。与对照相比，在干旱胁迫期间，叶片萎蔫或叶面积减少的显著延迟、黄色积累的减少和/或生长速率的增加将被视为玉米中基因功能增强耐旱性的证据。
实例5
水稻耐旱基因在拟南芥中实验室干旱筛选
为了了解水稻耐旱基因是否能提高双子叶植物的耐旱性或其他性状，本文所述的水稻表达载体可通过农杆菌介导的转化程序，使用花浸法转化为拟南芥(Columbia)，并鉴定了转基因植物(Clough，S.T.和Bent，A.F.(1998)The Plant Journal,16,735–743；Zhang，X.等,(2006)Nature Protocols,1:641-646)。
再生植株的后代，如T1植株，可能受到基于土壤的干旱胁迫。通过图像分析，可以在干旱胁迫之前和期间多次测量植物的面积、体积、生长速率和颜色。干旱胁迫期间，与对照相比，叶片萎蔫或叶面积减少的显著延迟、黄色积累的减少和/或生长速率的增加将被视为双子叶植物中基因功能增强抗旱性的证据。
序列表
<110> 未名生物农业集团有限公司
先锋海外公司
<120> 非生物胁迫耐性植物和方法
<130> 2019.07.25
<160> 158
<170> PatentIn version 3.5
<210> 1
<211> 3085
<212> DNA
<213> Oryza sativa
<400> 1
ggcgcgaatt ccggcgaggt attcgccgcg ttgccatggg gaagatgctg gatccgaagc 60
gtctcatgtc tgcgcgacag gagcggcatc gccgtcggcg gcgacggcaa ctccgacccc 120
gcagtagccc tccctctcct accttcttct tcttcagcaa ttgtgtttga tagccacgat 180
tgcttttttg gggaaaaaaa agagcatctc gcttgcaagc aattcaagaa ggggcaatgg 240
gacattatga tctgtggaat ttaggaacaa aatagtgtag aattgttttt ttcttttggg 300
aggtaaaagg tagcaatgct cggttgaatt tgatttgtag gaagtaatga aacaaattgg 360
agtgaatacc cgctcttttc acaaaaagct ggacaactga accaaattga attgttcgtg 420
ttcattcatg tgctttgatc tgatgttttc ccctgttgat tgatgtctgc atataaccac 480
gcaaatgatg tgtttgttca attgcttcac tggctaaaag gaagggctca ttctgtcacc 540
acgttgcaga ttctgaaggc gccaaaagaa caagatattc agtgcctagc cttccagagg 600
tatggtacct aatgctgata agaaatactt atattagaag caaagtgggt cactatgatt 660
ctatgactgc tctaagtagt ctctaactac acactggtgg tcatatggtg gctagatcaa 720
aaggattgag atcctctacc ttcaaactca aaggtaccat gtctttgtca ctgacagatg 780
ggtccatggt tcattgggcc tacatgttag tgacaaaggc acgtgccttt gaagttagag 840
gagcctgatc cagatttatg aacagatatt tatagctgca atcttctaca attctgcatg 900
agggcacctt ttctctgttc tagctgttgc acctttatca ctcaagcaga aaattgtttg 960
tgatggcgtc accttaataa tcttgttaag gcagttcact ttctaaaggt tcttcctcac 1020
ttatcagcgg gttcctgtac ttcttttatg tgattactct ggtctcgacc agtttcctga 1080
tgatcaatgt ttatgtttac cagaaccccc aatgtgcgat ttgtccttaa gatacatgtt 1140
tatttttttc ttcattacct ttgactaatt tataggatga tattgtaggg agaaaagcat 1200
catttcatac ataacattgt tgactaccgc tgcatacttc aagcaagaat aggtttcata 1260
ctatgtgatc ataattagtc actggctaca atctgcttta tctttttccc gatagccact 1320
aagaaaactt tgaaagggaa aaggagaaaa atgaaaatgt gccgttcagt ttgcgtacca 1380
caaatcccaa agagctattc cttataagct actaatagta ctgctttccc ttccaattga 1440
accttcacaa ttcttttaaa aaaacttttg tccttgctag tgcttgtagg aaatctggtg 1500
ccatatacac tccctaatgt cattcaaaga tgctgctcgt gcagcatgcg tgtctcgtgc 1560
ttttcgtcat tcctggcgat gccatcccaa tcttatatgt tgtattggaa tactgggctc 1620
ggatttcatc aacaaatttg accgcattat gaaaaatcac tcaggaattg gcatcaagtc 1680
ggtaaagttt caatataata gtttttacaa tactagaagg agcactagca tcagccatca 1740
tttcgatagt tggcatcaga ttgctattac accgtggatc gaagaactca ccattagcct 1800
atctctatct tcattcaata tggagtacag cttcccatgc tcacttctag ctgatgggag 1860
agcaagctca atgcggcatc tttatcttgg caattgtgga ttccatccca caatcaacct 1920
tgacttgaga aacctgacaa ggttgcatct gattaatgta catattacag gagatgagtt 1980
agggtgcctt ctctcaaatt ctcattgttt ggagcagtta gaacttatgt attgcaatgg 2040
aataatttgt ctgaagatac cttccctgtt gcagtgcctc agccacctag aagtgtttga 2100
ttgcagaatt caagtagttg agaataaggc tccaaatctt tgcagttttg actttggagg 2160
taggcaggta caactcttac ttggcgaatc gttgcgaatg aagagactat ccttgcacta 2220
ccccgatgct atgtattatg ctcatgcaaa gcttccatcc aatgcgccaa atcttgaaac 2280
tctcaccctc tgatgctcga ctgacaaggt actcaaaaaa cataaatgat catagacttg 2340
tgatgtccat ataacaatat tcatgccttc agcctgatga tctttatgct ttttatgcag 2400
atggttgata caccaatatt gcctagcaaa ttcctccatc tcaagtgctt gaccattgat 2460
cataccatta tctcatcttc cccagcttat aattatcttt acctggttta ttttctcgac 2520
gcttgccctt ccttggagac tttccttttg ggtgtaagtc aattttaatc ctgcaaactt 2580
atgattaagt gactagccca tcaaatgaaa aagtttatta ttggttttca tgtccagata 2640
tcgcagaacc acatggagca tgactcgatt attggagatt cctcacatat gaggcaaatg 2700
ccaggacacc gtcatgacaa cctccggagt gtggagatca ccggtttcta ctctgcaaag 2760
agcttgatcg agctaacatg ttatattctt gacaatacag ttgcactcaa gtaccttaca 2820
ctggatacaa ctcgtggttt tttcagctgt tctacaggtg aacacgacag atgctttcct 2880
atggacaaaa tcatgataac ggaagccaaa agggcagtcc tggctatcca aacatacatc 2940
gagcgaaaag ttccttccac ggtcaagcta aatgttgtga agccttgcag ccgttgccat 3000
gctgttgaat catatttgta attattattt ttcttcaact taattttctt ggtaattcgg 3060
tgattttaaa gtgtccggtg ctttc 3085
<210> 2
<211> 1236
<212> DNA
<213> Oryza sativa
<400> 2
atggggaaga tgctggatcc gaagcgtctc atgtctgcgc gacaggagcg gcatcgccgt 60
cggcggcgac ggcaactccg accccgcaat tctgaaggcg ccaaaagaac aagatattca 120
gtgcctagcc ttccagagga aatctggtgc catatacact ccctaatgtc attcaaagat 180
gctgctcgtg cagcatgcgt gtctcgtgct tttcgtcatt cctggcgatg ccatcccaat 240
cttatatgtt gtattggaat actgggctcg gatttcatca acaaatttga ccgcattatg 300
aaaaatcact caggaattgg catcaagtcg gtaaagtttc aatataatag tttttacaat 360
actagaagga gcactagcat cagccatcat ttcgatagtt ggcatcagat tgctattaca 420
ccgtggatcg aagaactcac cattagccta tctctatctt cattcaatat ggagtacagc 480
ttcccatgct cacttctagc tgatgggaga gcaagctcaa tgcggcatct ttatcttggc 540
aattgtggat tccatcccac aatcaacctt gacttgagaa acctgacaag gttgcatctg 600
attaattgcc tcagccacct agaagtgttt gattgcagaa ttcaagtagt tgagaataag 660
gctccaaatc tttgcagttt tgactttgga ggtaggcaga tggttgatac accaatattg 720
cctagcaaat tcctccatct caagtgcttg accattgatc ataccattat ctcatcttcc 780
ccagcttata attatcttta cctggtttat tttctcgacg cttgcccttc cttggagact 840
ttccttttgg gtatatcgca gaaccacatg gagcatgact cgattattgg agattcctca 900
catatgaggc aaatgccagg acaccgtcat gacaacctcc ggagtgtgga gatcaccggt 960
ttctactctg caaagagctt gatcgagcta acatgttata ttcttgacaa tacagttgca 1020
ctcaagtacc ttacactgga tacaactcgt ggttttttca gctgttctac aggtgaacac 1080
gacagatgct ttcctatgga caaaatcatg ataacggaag ccaaaagggc agtcctggct 1140
atccaaacat acatcgagcg aaaagttcct tccacggtca agctaaatgt tgtgaagcct 1200
tgcagccgtt gccatgctgt tgaatcatat ttgtaa 1236
<210> 3
<211> 411
<212> PRT
<213> Oryza sativa
<400> 3
Met Gly Lys Met Leu Asp Pro Lys Arg Leu Met Ser Ala Arg Gln Glu
1 5 10 15
Arg His Arg Arg Arg Arg Arg Arg Gln Leu Arg Pro Arg Asn Ser Glu
20 25 30
Gly Ala Lys Arg Thr Arg Tyr Ser Val Pro Ser Leu Pro Glu Glu Ile
35 40 45
Trp Cys His Ile His Ser Leu Met Ser Phe Lys Asp Ala Ala Arg Ala
50 55 60
Ala Cys Val Ser Arg Ala Phe Arg His Ser Trp Arg Cys His Pro Asn
65 70 75 80
Leu Ile Cys Cys Ile Gly Ile Leu Gly Ser Asp Phe Ile Asn Lys Phe
85 90 95
Asp Arg Ile Met Lys Asn His Ser Gly Ile Gly Ile Lys Ser Val Lys
100 105 110
Phe Gln Tyr Asn Ser Phe Tyr Asn Thr Arg Arg Ser Thr Ser Ile Ser
115 120 125
His His Phe Asp Ser Trp His Gln Ile Ala Ile Thr Pro Trp Ile Glu
130 135 140
Glu Leu Thr Ile Ser Leu Ser Leu Ser Ser Phe Asn Met Glu Tyr Ser
145 150 155 160
Phe Pro Cys Ser Leu Leu Ala Asp Gly Arg Ala Ser Ser Met Arg His
165 170 175
Leu Tyr Leu Gly Asn Cys Gly Phe His Pro Thr Ile Asn Leu Asp Leu
180 185 190
Arg Asn Leu Thr Arg Leu His Leu Ile Asn Cys Leu Ser His Leu Glu
195 200 205
Val Phe Asp Cys Arg Ile Gln Val Val Glu Asn Lys Ala Pro Asn Leu
210 215 220
Cys Ser Phe Asp Phe Gly Gly Arg Gln Met Val Asp Thr Pro Ile Leu
225 230 235 240
Pro Ser Lys Phe Leu His Leu Lys Cys Leu Thr Ile Asp His Thr Ile
245 250 255
Ile Ser Ser Ser Pro Ala Tyr Asn Tyr Leu Tyr Leu Val Tyr Phe Leu
260 265 270
Asp Ala Cys Pro Ser Leu Glu Thr Phe Leu Leu Gly Ile Ser Gln Asn
275 280 285
His Met Glu His Asp Ser Ile Ile Gly Asp Ser Ser His Met Arg Gln
290 295 300
Met Pro Gly His Arg His Asp Asn Leu Arg Ser Val Glu Ile Thr Gly
305 310 315 320
Phe Tyr Ser Ala Lys Ser Leu Ile Glu Leu Thr Cys Tyr Ile Leu Asp
325 330 335
Asn Thr Val Ala Leu Lys Tyr Leu Thr Leu Asp Thr Thr Arg Gly Phe
340 345 350
Phe Ser Cys Ser Thr Gly Glu His Asp Arg Cys Phe Pro Met Asp Lys
355 360 365
Ile Met Ile Thr Glu Ala Lys Arg Ala Val Leu Ala Ile Gln Thr Tyr
370 375 380
Ile Glu Arg Lys Val Pro Ser Thr Val Lys Leu Asn Val Val Lys Pro
385 390 395 400
Cys Ser Arg Cys His Ala Val Glu Ser Tyr Leu
405 410
<210> 4
<211> 1071
<212> DNA
<213> Oryza sativa
<400> 4
atgtcattca aagatgctgc tcgtgcagca tgcgtgtctc gtgcttttcg tcattcctgg 60
cgatgccatc ccaatcttat atgttgtatt ggaatactgg gctcggattt catcaacaaa 120
tttgaccgca ttatgaaaaa tcactcagga attggcatca agtcggtaaa gtttcaatat 180
aatagttttt acaatactag aaggagcact agcatcagcc atcatttcga tagttggcat 240
cagattgcta ttacaccgtg gatcgaagaa ctcaccatta gcctatctct atcttcattc 300
aatatggagt acagcttccc atgctcactt ctagctgatg ggagagcaag ctcaatgcgg 360
catctttatc ttggcaattg tggattccat cccacaatca accttgactt gagaaacctg 420
acaaggttgc atctgattaa ttgcctcagc cacctagaag tgtttgattg cagaattcaa 480
gtagttgaga ataaggctcc aaatctttgc agttttgact ttggaggtag gcagatggtt 540
gatacaccaa tattgcctag caaattcctc catctcaagt gcttgaccat tgatcatacc 600
attatctcat cttccccagc ttataattat ctttacctgg tttattttct cgacgcttgc 660
ccttccttgg agactttcct tttgggtata tcgcagaacc acatggagca tgactcgatt 720
attggagatt cctcacatat gaggcaaatg ccaggacacc gtcatgacaa cctccggagt 780
gtggagatca ccggtttcta ctctgcaaag agcttgatcg agctaacatg ttatattctt 840
gacaatacag ttgcactcaa gtaccttaca ctggatacaa ctcgtggttt tttcagctgt 900
tctacaggtg aacacgacag atgctttcct atggacaaaa tcatgataac ggaagccaaa 960
agggcagtcc tggctatcca aacatacatc gagcgaaaag ttccttccac ggtcaagcta 1020
aatgttgtga agccttgcag ccgttgccat gctgttgaat catatttgta a 1071
<210> 5
<211> 356
<212> PRT
<213> Oryza sativa
<400> 5
Met Ser Phe Lys Asp Ala Ala Arg Ala Ala Cys Val Ser Arg Ala Phe
1 5 10 15
Arg His Ser Trp Arg Cys His Pro Asn Leu Ile Cys Cys Ile Gly Ile
20 25 30
Leu Gly Ser Asp Phe Ile Asn Lys Phe Asp Arg Ile Met Lys Asn His
35 40 45
Ser Gly Ile Gly Ile Lys Ser Val Lys Phe Gln Tyr Asn Ser Phe Tyr
50 55 60
Asn Thr Arg Arg Ser Thr Ser Ile Ser His His Phe Asp Ser Trp His
65 70 75 80
Gln Ile Ala Ile Thr Pro Trp Ile Glu Glu Leu Thr Ile Ser Leu Ser
85 90 95
Leu Ser Ser Phe Asn Met Glu Tyr Ser Phe Pro Cys Ser Leu Leu Ala
100 105 110
Asp Gly Arg Ala Ser Ser Met Arg His Leu Tyr Leu Gly Asn Cys Gly
115 120 125
Phe His Pro Thr Ile Asn Leu Asp Leu Arg Asn Leu Thr Arg Leu His
130 135 140
Leu Ile Asn Cys Leu Ser His Leu Glu Val Phe Asp Cys Arg Ile Gln
145 150 155 160
Val Val Glu Asn Lys Ala Pro Asn Leu Cys Ser Phe Asp Phe Gly Gly
165 170 175
Arg Gln Met Val Asp Thr Pro Ile Leu Pro Ser Lys Phe Leu His Leu
180 185 190
Lys Cys Leu Thr Ile Asp His Thr Ile Ile Ser Ser Ser Pro Ala Tyr
195 200 205
Asn Tyr Leu Tyr Leu Val Tyr Phe Leu Asp Ala Cys Pro Ser Leu Glu
210 215 220
Thr Phe Leu Leu Gly Ile Ser Gln Asn His Met Glu His Asp Ser Ile
225 230 235 240
Ile Gly Asp Ser Ser His Met Arg Gln Met Pro Gly His Arg His Asp
245 250 255
Asn Leu Arg Ser Val Glu Ile Thr Gly Phe Tyr Ser Ala Lys Ser Leu
260 265 270
Ile Glu Leu Thr Cys Tyr Ile Leu Asp Asn Thr Val Ala Leu Lys Tyr
275 280 285
Leu Thr Leu Asp Thr Thr Arg Gly Phe Phe Ser Cys Ser Thr Gly Glu
290 295 300
His Asp Arg Cys Phe Pro Met Asp Lys Ile Met Ile Thr Glu Ala Lys
305 310 315 320
Arg Ala Val Leu Ala Ile Gln Thr Tyr Ile Glu Arg Lys Val Pro Ser
325 330 335
Thr Val Lys Leu Asn Val Val Lys Pro Cys Ser Arg Cys His Ala Val
340 345 350
Glu Ser Tyr Leu
355
<210> 6
<211> 1174
<212> DNA
<213> Oryza sativa
<400> 6
ccatgttacg agcttctgga atacaatggg atcttcgtaa agttgatctt tacgagtctt 60
acaatcaatt cgattggaaa atccaatggc aaaaagaagg ggattcatta gcacgctatt 120
tagtacgaat cggtgaaacg agggaatcaa tcaaaattat tcaacaggct gtagaaaaaa 180
ttcctgggag cccttatgag aatttagaag tccgacgctt taagaaagca aagaattcca 240
aatggaatga ttttgaatat cgatttcttg gtaaaaaacc ttcacccaat tttgaattgt 300
caaaacaaga gtggaagccc caaaaggtga attaggaatt tatctggtag gagatgatag 360
tcttttcccc tggagatgga aaattcgtcc acccggtttt attaatttgc aaattcttcc 420
tcagcaagtc aaaaaaatga attggctgat atcagcgggc attctaggcc atgacctaga 480
gcctaagatt taagggttaa ttggttccat accactgcaa ctttaccaaa ttaaaaaaat 540
accactacta tccatcatat cgctgctggg tgccactgca attttagaaa attggatcca 600
tgccactccg tcgcattttc catccactcc tgttgatgcc gtctctccgc gtctaaggac 660
cgagccagag gacaccacca ccgacttcgg cttcggcaac gacaccgacg caggcgcgag 720
cagcggaggc gactcagggt tctcgtggtg gcgcaggcgc gcaagtggcg gccggaatcc 780
cgtgcggagc ggcccccatg ccacgacggc ggttgaggat gccgcagggg agcgatgcag 840
ctgctcctcg tcgcctcgcc ggtcattcgc cgctgcaagg acctcgtcgc gcgtgccctc 900
ggccagggac gccgacgcca ccggccggag acggcggcgc agccgcggcc cactctgccg 960
cctcctcctt ggatgcctcg gcttcctcgt cgcatgcccc gacaacacga tggtcgccga 1020
gtcgacgccc gtgaggtggc ttccgctgtc agtgtcgtca acggcgaggc tgacctgggt 1080
ggcggcgaag gagctcgacc gcggtgaggg agctcggcgg tggcggcgag ggagctcggc 1140
accggagacc gcaccagaga gaccccacta ggcc 1174
<210> 7
<211> 852
<212> DNA
<213> Oryza sativa
<400> 7
atgttacgag cttctggaat acaatgggat cttcgtaaag ttgatcttta cgagtcttac 60
aatcaattcg attggaaaat ccaatggcaa aaagaagggg attcattagc acgctattta 120
gtacgaatcg gtgaaacgag ggaatcaatc aaaattattc aacaggctgt agaaaaaatt 180
cctgggagcc cttatgagaa tttagaagtc cgacgcttta agaaagcaaa gaattccaaa 240
tggaatgatt ttgaatatcg atttcttgaa aattggatcc atgccactcc gtcgcatttt 300
ccatccactc ctgttgatgc cgtctctccg cgtctaagga ccgagccaga ggacaccacc 360
accgacttcg gcttcggcaa cgacaccgac gcaggcgcga gcagcggagg cgactcaggg 420
ttctcgtggt ggcgcaggcg cgcaagtggc ggccggaatc ccgtgcggag cggcccccat 480
gccacgacgg cggttgagga tgccgcaggg gagcgatgca gctgctcctc gtcgcctcgc 540
cggtcattcg ccgctgcaag gacctcgtcg cgcgtgccct cggccaggga cgccgacgcc 600
accggccgga gacggcggcg cagccgcggc ccactctgcc gcctcctcct tggatgcctc 660
ggcttcctcg tcgcatgccc cgacaacacg atggtcgccg agtcgacgcc cgtgaggtgg 720
cttccgctgt cagtgtcgtc aacggcgagg ctgacctggg tggcggcgaa ggagctcgac 780
cgcggtgagg gagctcggcg gtggcggcga gggagctcgg caccggagac cgcaccagag 840
agaccccact ag 852
<210> 8
<211> 283
<212> PRT
<213> Oryza sativa
<400> 8
Met Leu Arg Ala Ser Gly Ile Gln Trp Asp Leu Arg Lys Val Asp Leu
1 5 10 15
Tyr Glu Ser Tyr Asn Gln Phe Asp Trp Lys Ile Gln Trp Gln Lys Glu
20 25 30
Gly Asp Ser Leu Ala Arg Tyr Leu Val Arg Ile Gly Glu Thr Arg Glu
35 40 45
Ser Ile Lys Ile Ile Gln Gln Ala Val Glu Lys Ile Pro Gly Ser Pro
50 55 60
Tyr Glu Asn Leu Glu Val Arg Arg Phe Lys Lys Ala Lys Asn Ser Lys
65 70 75 80
Trp Asn Asp Phe Glu Tyr Arg Phe Leu Glu Asn Trp Ile His Ala Thr
85 90 95
Pro Ser His Phe Pro Ser Thr Pro Val Asp Ala Val Ser Pro Arg Leu
100 105 110
Arg Thr Glu Pro Glu Asp Thr Thr Thr Asp Phe Gly Phe Gly Asn Asp
115 120 125
Thr Asp Ala Gly Ala Ser Ser Gly Gly Asp Ser Gly Phe Ser Trp Trp
130 135 140
Arg Arg Arg Ala Ser Gly Gly Arg Asn Pro Val Arg Ser Gly Pro His
145 150 155 160
Ala Thr Thr Ala Val Glu Asp Ala Ala Gly Glu Arg Cys Ser Cys Ser
165 170 175
Ser Ser Pro Arg Arg Ser Phe Ala Ala Ala Arg Thr Ser Ser Arg Val
180 185 190
Pro Ser Ala Arg Asp Ala Asp Ala Thr Gly Arg Arg Arg Arg Arg Ser
195 200 205
Arg Gly Pro Leu Cys Arg Leu Leu Leu Gly Cys Leu Gly Phe Leu Val
210 215 220
Ala Cys Pro Asp Asn Thr Met Val Ala Glu Ser Thr Pro Val Arg Trp
225 230 235 240
Leu Pro Leu Ser Val Ser Ser Thr Ala Arg Leu Thr Trp Val Ala Ala
245 250 255
Lys Glu Leu Asp Arg Gly Glu Gly Ala Arg Arg Trp Arg Arg Gly Ser
260 265 270
Ser Ala Pro Glu Thr Ala Pro Glu Arg Pro His
275 280
<210> 9
<211> 1333
<212> DNA
<213> Oryza sativa
<400> 9
cttcccgggc ctcttcaaaa atccccgcgc cgcgtagccg atcacttgtc gcaccaacat 60
ggaggaggac ggcggcagcg acgcgcgggt ggtggagtcc atgcagcggc tgctcgacgc 120
ggtgccgccc ggtgccgacg acccgtacac catcttccgc ctccccgcgg ccgtgcggga 180
gcggcaccgc gacctgtacg agccaaagct cgtgtccgtg gggccgtact accacggccg 240
cgacgggctc ggcgccgcgc agcgccacaa gtggcgcctc ctccgcgact tcctgtcgcg 300
gcagagcgac gacaaggccg ggctcggagc ctacgtgcgc gccgcgcggg cggtcgaggc 360
cgacgcgcgg cagtgctacg cggaggggtt cgacgacgtg ggcgccgacg agttcgccga 420
gatgctggtg ctcgacggtt gcttcctgct cgagttcttc ctgaggaaga gcgaggggca 480
gctcgcggcg cccgggggcg ccaagtgggc gtggcagcac atgtaccacg acgtcctcct 540
cctggagaat cagataccgt tcttcgtcgt cgagaggctg cacggcgtcg cctttgccgg 600
ggacgatgat ggcgccgccg accgtgacgc gctcctcgac atcttctgca aggcgttcgc 660
cggcgacctg ccttcgagcc gcgtcatacg gccgccgagc gacaagacca tccaccacct 720
gctgcacctg cactacgagt gcaacgtccg caacccggcc gcggacagcg acaaggcgcg 780
caacggcggg gacgccgcca acggcggcgc gtcgtcgctg gccatctgga agcagccgcc 840
cgtcccgtcc ccgcgctcca gcgacggcgc catcaaaggg cggatgacgt cgatgatccc 900
gccggcggcc aagatggagg aggccggcgt gacgttcaag cggaaggcca ccccgcgcga 960
cgtgttcgac atgagcttcc ggtacggcgt gctgcacatg ccggcgttcg tggtggacga 1020
gggcgccaag gtgctcctcg cgaacctggt ggcgttcgag cagggcggcg gccgcgcggc 1080
gcggaagctg gaagggggca acctggcgac ggggttcgtg gcgctgctgg gttcgctggt 1140
gaactcgcgg cgggacgtgg aggtgctccg ccgctgcggg atcctgcact gcatggtcac 1200
cgacgaggag gccgtggcgt acttcagcca cgtggtgcag tacacgacca tggactacga 1260
ccggcacctg ctggcttgcc tgttccggga catccgggag cactgccact ggaaccgatg 1320
atttgggtgt ttc 1333
<210> 10
<211> 1263
<212> DNA
<213> Oryza sativa
<400> 10
atggaggagg acggcggcag cgacgcgcgg gtggtggagt ccatgcagcg gctgctcgac 60
gcggtgccgc ccggtgccga cgacccgtac accatcttcc gcctccccgc ggccgtgcgg 120
gagcggcacc gcgacctgta cgagccaaag ctcgtgtccg tggggccgta ctaccacggc 180
cgcgacgggc tcggcgccgc gcagcgccac aagtggcgcc tcctccgcga cttcctgtcg 240
cggcagagcg acgacaaggc cgggctcgga gcctacgtgc gcgccgcgcg ggcggtcgag 300
gccgacgcgc ggcagtgcta cgcggagggg ttcgacgacg tgggcgccga cgagttcgcc 360
gagatgctgg tgctcgacgg ttgcttcctg ctcgagttct tcctgaggaa gagcgagggg 420
cagctcgcgg cgcccggggg cgccaagtgg gcgtggcagc acatgtacca cgacgtcctc 480
ctcctggaga atcagatacc gttcttcgtc gtcgagaggc tgcacggcgt cgcctttgcc 540
ggggacgatg atggcgccgc cgaccgtgac gcgctcctcg acatcttctg caaggcgttc 600
gccggcgacc tgccttcgag ccgcgtcata cggccgccga gcgacaagac catccaccac 660
ctgctgcacc tgcactacga gtgcaacgtc cgcaacccgg ccgcggacag cgacaaggcg 720
cgcaacggcg gggacgccgc caacggcggc gcgtcgtcgc tggccatctg gaagcagccg 780
cccgtcccgt ccccgcgctc cagcgacggc gccatcaaag ggcggatgac gtcgatgatc 840
ccgccggcgg ccaagatgga ggaggccggc gtgacgttca agcggaaggc caccccgcgc 900
gacgtgttcg acatgagctt ccggtacggc gtgctgcaca tgccggcgtt cgtggtggac 960
gagggcgcca aggtgctcct cgcgaacctg gtggcgttcg agcagggcgg cggccgcgcg 1020
gcgcggaagc tggaaggggg caacctggcg acggggttcg tggcgctgct gggttcgctg 1080
gtgaactcgc ggcgggacgt ggaggtgctc cgccgctgcg ggatcctgca ctgcatggtc 1140
accgacgagg aggccgtggc gtacttcagc cacgtggtgc agtacacgac catggactac 1200
gaccggcacc tgctggcttg cctgttccgg gacatccggg agcactgcca ctggaaccga 1260
tga 1263
<210> 11
<211> 420
<212> PRT
<213> Oryza sativa
<400> 11
Met Glu Glu Asp Gly Gly Ser Asp Ala Arg Val Val Glu Ser Met Gln
1 5 10 15
Arg Leu Leu Asp Ala Val Pro Pro Gly Ala Asp Asp Pro Tyr Thr Ile
20 25 30
Phe Arg Leu Pro Ala Ala Val Arg Glu Arg His Arg Asp Leu Tyr Glu
35 40 45
Pro Lys Leu Val Ser Val Gly Pro Tyr Tyr His Gly Arg Asp Gly Leu
50 55 60
Gly Ala Ala Gln Arg His Lys Trp Arg Leu Leu Arg Asp Phe Leu Ser
65 70 75 80
Arg Gln Ser Asp Asp Lys Ala Gly Leu Gly Ala Tyr Val Arg Ala Ala
85 90 95
Arg Ala Val Glu Ala Asp Ala Arg Gln Cys Tyr Ala Glu Gly Phe Asp
100 105 110
Asp Val Gly Ala Asp Glu Phe Ala Glu Met Leu Val Leu Asp Gly Cys
115 120 125
Phe Leu Leu Glu Phe Phe Leu Arg Lys Ser Glu Gly Gln Leu Ala Ala
130 135 140
Pro Gly Gly Ala Lys Trp Ala Trp Gln His Met Tyr His Asp Val Leu
145 150 155 160
Leu Leu Glu Asn Gln Ile Pro Phe Phe Val Val Glu Arg Leu His Gly
165 170 175
Val Ala Phe Ala Gly Asp Asp Asp Gly Ala Ala Asp Arg Asp Ala Leu
180 185 190
Leu Asp Ile Phe Cys Lys Ala Phe Ala Gly Asp Leu Pro Ser Ser Arg
195 200 205
Val Ile Arg Pro Pro Ser Asp Lys Thr Ile His His Leu Leu His Leu
210 215 220
His Tyr Glu Cys Asn Val Arg Asn Pro Ala Ala Asp Ser Asp Lys Ala
225 230 235 240
Arg Asn Gly Gly Asp Ala Ala Asn Gly Gly Ala Ser Ser Leu Ala Ile
245 250 255
Trp Lys Gln Pro Pro Val Pro Ser Pro Arg Ser Ser Asp Gly Ala Ile
260 265 270
Lys Gly Arg Met Thr Ser Met Ile Pro Pro Ala Ala Lys Met Glu Glu
275 280 285
Ala Gly Val Thr Phe Lys Arg Lys Ala Thr Pro Arg Asp Val Phe Asp
290 295 300
Met Ser Phe Arg Tyr Gly Val Leu His Met Pro Ala Phe Val Val Asp
305 310 315 320
Glu Gly Ala Lys Val Leu Leu Ala Asn Leu Val Ala Phe Glu Gln Gly
325 330 335
Gly Gly Arg Ala Ala Arg Lys Leu Glu Gly Gly Asn Leu Ala Thr Gly
340 345 350
Phe Val Ala Leu Leu Gly Ser Leu Val Asn Ser Arg Arg Asp Val Glu
355 360 365
Val Leu Arg Arg Cys Gly Ile Leu His Cys Met Val Thr Asp Glu Glu
370 375 380
Ala Val Ala Tyr Phe Ser His Val Val Gln Tyr Thr Thr Met Asp Tyr
385 390 395 400
Asp Arg His Leu Leu Ala Cys Leu Phe Arg Asp Ile Arg Glu His Cys
405 410 415
His Trp Asn Arg
420
<210> 12
<211> 1796
<212> DNA
<213> Oryza sativa
<400> 12
ctcgctctcc attcccaaat ctcctccgca tcgggatggc gcaggtggtg gctgcggcag 60
ggacggcggc ggcggtggcg gcggtgggga ggccgctggg tggcggcgga tctggggccg 120
acgcgctccg cccggcggcg aggctgtcgt tcgcgccgcg ctggtgcggt gggagcgcgg 180
gggcagcaag ggcgcgccgc gagtccgccg tcacgtccgt gattagccgc gccccgcgtc 240
tcgacgccga ggtgctgccg gtgtcggccg acgacgacgc ggacgtgaag gaagaagaaa 300
acttccagca tctcaaggcc attcagcaac ttgcaacatc agccaatggt gtgtggtcta 360
aaccaaatgt gagacgcaag acaaagatcg tgtgcaccat tggtccttca actaacacaa 420
aggagatgat atggaaactt gctgaggctg gcatgaatgt ggctcggctt aatatgtcac 480
atggagacca tgcatcacac cagaaagtta ttgacttagt aaaggaatat aatgctcaaa 540
caaaggataa cgtgattgct attatgcttg acacgaaggg accagaagtt aggagtggag 600
atttgcctca accaataatg ttggaaactg gccaggagtt cactttcaca attaagagag 660
gagttgggac tgagacatgt gttagcgtta actatgatga ctttgttaat gatgtagaag 720
tgggtgacat gctcctcgta gatggtggaa tgatgtcgtt gttggtcaag tccaaaactg 780
aagattcggt aaaatgtgaa gttattgatg gaggtgaact gaaatccagg cgtcacctga 840
atgttcgtgg aaagagcgca accttgccat caataactga caaggattgg gatgatatta 900
agtttggtgt ggagaatcaa gttgactact atgccgtttc ttttgtcaaa gatgctcaag 960
ttgtgcatga attgaaggat tatctcagaa gctccaatgc cgatatacat gtaattgtaa 1020
agattgaaag tgcagattcc attccaaacc tacactcaat catcacagca tcggatgggg 1080
ctatggttgc cagaggtgac ttgggagctg aactccctat tgaggaggta ccgctgctgc 1140
aggaagaaat tattagaatg tgcaggagca tgggaaaagc tgttattgtt gccactaata 1200
tgctagaaag tatgattgtt catccaactc caacccgagc agaagtttca gacattgcta 1260
tagctgttcg agaaggttct gatggcatta tgctttctgg ggaaactgca cacggaaagt 1320
ttcctttgaa agctgttaag gtcatgcata ctgttgccct gagaactgaa gccactatgt 1380
ctggtgggga aacaccggct aaccttggtc aggtgttcaa gaatcacatg agtgaaatgt 1440
ttgcatacca ttcaacaatg atgtccaaca ctcttggaac atcaattgtg gttttcacca 1500
ggacaggatt tatggctatc ctacttagtc attaccgccc ttctggcacc atttttgcct 1560
ttacggacca ggagagagtt agacaacgat tggctttgta ccaaggtgta tgtccagtcc 1620
agatggaatt ttctgatgat gctgagaaga catttggtga cgctttgtca tacttgctga 1680
aacatggtat ggtgaaggaa ggcgaggagg ttgcacttgt tcaaagtggc aggcaaccta 1740
tctggcggtc gcagtccacc cataatattc aggtcaggaa ggtttgatgt gaagac 1796
<210> 13
<211> 1752
<212> DNA
<213> Oryza sativa
<400> 13
atggcgcagg tggtggctgc ggcagggacg gcggcggcgg tggcggcggt ggggaggccg 60
ctgggtggcg gcggatctgg ggccgacgcg ctccgcccgg cggcgaggct gtcgttcgcg 120
ccgcgctggt gcggtgggag cgcgggggca gcaagggcgc gccgcgagtc cgccgtcacg 180
tccgtgatta gccgcgcccc gcgtctcgac gccgaggtgc tgccggtgtc ggccgacgac 240
gacgcggacg tgaaggaaga agaaaacttc cagcatctca aggccattca gcaacttgca 300
acatcagcca atggtgtgtg gtctaaacca aatgtgagac gcaagacaaa gatcgtgtgc 360
accattggtc cttcaactaa cacaaaggag atgatatgga aacttgctga ggctggcatg 420
aatgtggctc ggcttaatat gtcacatgga gaccatgcat cacaccagaa agttattgac 480
ttagtaaagg aatataatgc tcaaacaaag gataacgtga ttgctattat gcttgacacg 540
aagggaccag aagttaggag tggagatttg cctcaaccaa taatgttgga aactggccag 600
gagttcactt tcacaattaa gagaggagtt gggactgaga catgtgttag cgttaactat 660
gatgactttg ttaatgatgt agaagtgggt gacatgctcc tcgtagatgg tggaatgatg 720
tcgttgttgg tcaagtccaa aactgaagat tcggtaaaat gtgaagttat tgatggaggt 780
gaactgaaat ccaggcgtca cctgaatgtt cgtggaaaga gcgcaacctt gccatcaata 840
actgacaagg attgggatga tattaagttt ggtgtggaga atcaagttga ctactatgcc 900
gtttcttttg tcaaagatgc tcaagttgtg catgaattga aggattatct cagaagctcc 960
aatgccgata tacatgtaat tgtaaagatt gaaagtgcag attccattcc aaacctacac 1020
tcaatcatca cagcatcgga tggggctatg gttgccagag gtgacttggg agctgaactc 1080
cctattgagg aggtaccgct gctgcaggaa gaaattatta gaatgtgcag gagcatggga 1140
aaagctgtta ttgttgccac taatatgcta gaaagtatga ttgttcatcc aactccaacc 1200
cgagcagaag tttcagacat tgctatagct gttcgagaag gttctgatgg cattatgctt 1260
tctggggaaa ctgcacacgg aaagtttcct ttgaaagctg ttaaggtcat gcatactgtt 1320
gccctgagaa ctgaagccac tatgtctggt ggggaaacac cggctaacct tggtcaggtg 1380
ttcaagaatc acatgagtga aatgtttgca taccattcaa caatgatgtc caacactctt 1440
ggaacatcaa ttgtggtttt caccaggaca ggatttatgg ctatcctact tagtcattac 1500
cgcccttctg gcaccatttt tgcctttacg gaccaggaga gagttagaca acgattggct 1560
ttgtaccaag gtgtatgtcc agtccagatg gaattttctg atgatgctga gaagacattt 1620
ggtgacgctt tgtcatactt gctgaaacat ggtatggtga aggaaggcga ggaggttgca 1680
cttgttcaaa gtggcaggca acctatctgg cggtcgcagt ccacccataa tattcaggtc 1740
aggaaggttt ga 1752
<210> 14
<211> 583
<212> PRT
<213> Oryza sativa
<400> 14
Met Ala Gln Val Val Ala Ala Ala Gly Thr Ala Ala Ala Val Ala Ala
1 5 10 15
Val Gly Arg Pro Leu Gly Gly Gly Gly Ser Gly Ala Asp Ala Leu Arg
20 25 30
Pro Ala Ala Arg Leu Ser Phe Ala Pro Arg Trp Cys Gly Gly Ser Ala
35 40 45
Gly Ala Ala Arg Ala Arg Arg Glu Ser Ala Val Thr Ser Val Ile Ser
50 55 60
Arg Ala Pro Arg Leu Asp Ala Glu Val Leu Pro Val Ser Ala Asp Asp
65 70 75 80
Asp Ala Asp Val Lys Glu Glu Glu Asn Phe Gln His Leu Lys Ala Ile
85 90 95
Gln Gln Leu Ala Thr Ser Ala Asn Gly Val Trp Ser Lys Pro Asn Val
100 105 110
Arg Arg Lys Thr Lys Ile Val Cys Thr Ile Gly Pro Ser Thr Asn Thr
115 120 125
Lys Glu Met Ile Trp Lys Leu Ala Glu Ala Gly Met Asn Val Ala Arg
130 135 140
Leu Asn Met Ser His Gly Asp His Ala Ser His Gln Lys Val Ile Asp
145 150 155 160
Leu Val Lys Glu Tyr Asn Ala Gln Thr Lys Asp Asn Val Ile Ala Ile
165 170 175
Met Leu Asp Thr Lys Gly Pro Glu Val Arg Ser Gly Asp Leu Pro Gln
180 185 190
Pro Ile Met Leu Glu Thr Gly Gln Glu Phe Thr Phe Thr Ile Lys Arg
195 200 205
Gly Val Gly Thr Glu Thr Cys Val Ser Val Asn Tyr Asp Asp Phe Val
210 215 220
Asn Asp Val Glu Val Gly Asp Met Leu Leu Val Asp Gly Gly Met Met
225 230 235 240
Ser Leu Leu Val Lys Ser Lys Thr Glu Asp Ser Val Lys Cys Glu Val
245 250 255
Ile Asp Gly Gly Glu Leu Lys Ser Arg Arg His Leu Asn Val Arg Gly
260 265 270
Lys Ser Ala Thr Leu Pro Ser Ile Thr Asp Lys Asp Trp Asp Asp Ile
275 280 285
Lys Phe Gly Val Glu Asn Gln Val Asp Tyr Tyr Ala Val Ser Phe Val
290 295 300
Lys Asp Ala Gln Val Val His Glu Leu Lys Asp Tyr Leu Arg Ser Ser
305 310 315 320
Asn Ala Asp Ile His Val Ile Val Lys Ile Glu Ser Ala Asp Ser Ile
325 330 335
Pro Asn Leu His Ser Ile Ile Thr Ala Ser Asp Gly Ala Met Val Ala
340 345 350
Arg Gly Asp Leu Gly Ala Glu Leu Pro Ile Glu Glu Val Pro Leu Leu
355 360 365
Gln Glu Glu Ile Ile Arg Met Cys Arg Ser Met Gly Lys Ala Val Ile
370 375 380
Val Ala Thr Asn Met Leu Glu Ser Met Ile Val His Pro Thr Pro Thr
385 390 395 400
Arg Ala Glu Val Ser Asp Ile Ala Ile Ala Val Arg Glu Gly Ser Asp
405 410 415
Gly Ile Met Leu Ser Gly Glu Thr Ala His Gly Lys Phe Pro Leu Lys
420 425 430
Ala Val Lys Val Met His Thr Val Ala Leu Arg Thr Glu Ala Thr Met
435 440 445
Ser Gly Gly Glu Thr Pro Ala Asn Leu Gly Gln Val Phe Lys Asn His
450 455 460
Met Ser Glu Met Phe Ala Tyr His Ser Thr Met Met Ser Asn Thr Leu
465 470 475 480
Gly Thr Ser Ile Val Val Phe Thr Arg Thr Gly Phe Met Ala Ile Leu
485 490 495
Leu Ser His Tyr Arg Pro Ser Gly Thr Ile Phe Ala Phe Thr Asp Gln
500 505 510
Glu Arg Val Arg Gln Arg Leu Ala Leu Tyr Gln Gly Val Cys Pro Val
515 520 525
Gln Met Glu Phe Ser Asp Asp Ala Glu Lys Thr Phe Gly Asp Ala Leu
530 535 540
Ser Tyr Leu Leu Lys His Gly Met Val Lys Glu Gly Glu Glu Val Ala
545 550 555 560
Leu Val Gln Ser Gly Arg Gln Pro Ile Trp Arg Ser Gln Ser Thr His
565 570 575
Asn Ile Gln Val Arg Lys Val
580
<210> 15
<211> 932
<212> DNA
<213> Oryza sativa
<400> 15
gaagacgacg actcactgct agctggtagc tgctgccggt ggtgccgatc gactgccgga 60
cggcgggcag agacgccatg ggagaggaga agagccagca gcggcggcgg cagggccacc 120
ctctcctgag gggaggcggc gcggggaagc aggccgggcg gcgttacacc cacggcttct 180
ccgcctccca gatggtcgcg ctcgccgccc tgtgcggcgc gctggcgccc tcgctcccgc 240
cggacacccg cgacgacgac gacgacgacg ccggaggcgg acgctatggc ggcgccggtg 300
cgtccgacgc caaggccgtc agggacttcc tcctcgcctc cgccgccgac ccgcccgtcc 360
ccgacgaggt tggtactaca actcggccta cgcgcttaac aagcttgctt ttgcttagct 420
acctgtagcg agagtgaagc ctcggccggc gatggtgcag gtggcggagc tgatgacgag 480
gatgtgcctc cgggaggcgc tggcgctggt gcgggcggtg ctgtggctgc tggggacgcg 540
gctggggacg ctggcgctgt gcggggggcg gtgcgtgtcg tgggggaggt ggccgttcgt 600
gctcacgttc gcggagatgc cggtggagcg gcgggaggag gcgctcgggc ggtggagcag 660
ggtgaccgtg ctcccgccgc tgcgcgcctt cttcctcgtc gtcaaggtct tctgcctcta 720
cgtcttctac tcctgggtaa ccactcctcc tcctcctcct ctcatggcct ctcgtacacc 780
gctgatcgat cagctgcgag ttcattcacg ttagattttt ttttctcgcg ttttgcccgc 840
gccacgcacg tgcgcgcggt gatttttctc tcgacggatg cacgtacgta cgcccgtgag 900
ggacggacta agcgttgtgg ggactgttta tg 932
<210> 16
<211> 645
<212> DNA
<213> Oryza sativa
<400> 16
atgggagagg agaagagcca gcagcggcgg cggcagggcc accctctcct gaggggaggc 60
ggcgcgggga agcaggccgg gcggcgttac acccacggct tctccgcctc ccagatggtc 120
gcgctcgccg ccctgtgcgg cgcgctggcg ccctcgctcc cgccggacac ccgcgacgac 180
gacgacgacg acgccggagg cggacgctat ggcggcgccg gtgcgtccga cgccaaggcc 240
gtcagggact tcctcctcgc ctccgccgcc gacccgcccg tccccgacga ggtggcggag 300
ctgatgacga ggatgtgcct ccgggaggcg ctggcgctgg tgcgggcggt gctgtggctg 360
ctggggacgc ggctggggac gctggcgctg tgcggggggc ggtgcgtgtc gtgggggagg 420
tggccgttcg tgctcacgtt cgcggagatg ccggtggagc ggcgggagga ggcgctcggg 480
cggtggagca gggtgaccgt gctcccgccg ctgcgcgcct tcttcctcgt cgtcaaggtc 540
ttctgcctct acgtcttcta ctcctgggta accactcctc ctcctcctcc tctcatggcc 600
tctcgtacac cgctgatcga tcagctgcga gttcattcac gttag 645
<210> 17
<211> 214
<212> PRT
<213> Oryza sativa
<400> 17
Met Gly Glu Glu Lys Ser Gln Gln Arg Arg Arg Gln Gly His Pro Leu
1 5 10 15
Leu Arg Gly Gly Gly Ala Gly Lys Gln Ala Gly Arg Arg Tyr Thr His
20 25 30
Gly Phe Ser Ala Ser Gln Met Val Ala Leu Ala Ala Leu Cys Gly Ala
35 40 45
Leu Ala Pro Ser Leu Pro Pro Asp Thr Arg Asp Asp Asp Asp Asp Asp
50 55 60
Ala Gly Gly Gly Arg Tyr Gly Gly Ala Gly Ala Ser Asp Ala Lys Ala
65 70 75 80
Val Arg Asp Phe Leu Leu Ala Ser Ala Ala Asp Pro Pro Val Pro Asp
85 90 95
Glu Val Ala Glu Leu Met Thr Arg Met Cys Leu Arg Glu Ala Leu Ala
100 105 110
Leu Val Arg Ala Val Leu Trp Leu Leu Gly Thr Arg Leu Gly Thr Leu
115 120 125
Ala Leu Cys Gly Gly Arg Cys Val Ser Trp Gly Arg Trp Pro Phe Val
130 135 140
Leu Thr Phe Ala Glu Met Pro Val Glu Arg Arg Glu Glu Ala Leu Gly
145 150 155 160
Arg Trp Ser Arg Val Thr Val Leu Pro Pro Leu Arg Ala Phe Phe Leu
165 170 175
Val Val Lys Val Phe Cys Leu Tyr Val Phe Tyr Ser Trp Val Thr Thr
180 185 190
Pro Pro Pro Pro Pro Leu Met Ala Ser Arg Thr Pro Leu Ile Asp Gln
195 200 205
Leu Arg Val His Ser Arg
210
<210> 18
<211> 2416
<212> DNA
<213> Oryza sativa
<400> 18
gtctctctcc agcttctctc tcccccatgg ctctcgcgtg cgcatcgcgc cgcctcctcg 60
ccggcgccgg aactccggcg agatccttcc actcccagcc ctaccaaggt aaccccctct 120
cgtctcctcc tcctcctccg cctcctccct cttggttatt gtgattggag ctcgattgct 180
gcgggcgcag ccaaggtcgg cgtggtggag ttcctcaacg gggtcgggaa gggggtggaa 240
acgcacgccg cgaaggtgga ggaggcggtg ggaggcgacc tccagaggct cctcgagacc 300
cgcacgctgc ggctcaagaa gctcggcatc ccctgcaagc atgtacgtcc tatacctgtc 360
tgtccctggt atgcctttgg tagtagagtc tctggttcat gtgcagattt gagcagcggc 420
ttgatgttgt tgccgattat gttagtgagt gggggtattc agcatttcgt aatgtgatgg 480
tatgtttttg gttagatttt gctgtataat gttgccattg ttggatgatg tcgttgcgtt 540
tgtttggtag tagtgaaccg agtaactgat ttctgtgtgc ttaaaactat ggctcgttgc 600
attatgcgta gatttgtttg aaaaatcata cgtactgttg attttagagt aaatttcaca 660
ataccacatg cttttggctc caagttgcac aaaaccctgg gggttttagc cctacgcact 720
taacaattat aagtgttgct ttttggggag agttcttggt attgaatcgg attggattgc 780
aatgtattcc ttggtcaata gattggacaa aatgtatttt ggcgtgtgct tggaggttaa 840
cattgttgga gagtaatttg gtaaatgtgc tatctaactc agtgttttgc ttttcctgct 900
tatttgtctc taggctgtgc atttctggat agacatttga catgaaaaat ctgaagttct 960
gtttttattt ttttctaaat agttgttctt gtgaataata aagctatgtc cgtgcaaaga 1020
tttttcaaaa gaatatacca ttgctttgag gataggtata tgttgtcagt tttcagaatc 1080
caattagcaa aagctaagta gttctgccat cgtgtcgtgc tttgaaggga acttacctct 1140
ttgacctgag atgttgatat gtatctttta ttattggtag caactggtag tgaattactc 1200
aatactcaat agagagttgt ggtattctgt agcatgttct tcactgggtg atcacatttt 1260
tacttgatta agccataaaa ttggcatttt tatgcttttt agatgtcttg gtgaatttgg 1320
caattatact gtgactttag aaatttgaag tgcaaaatac acattttttt ttccttcaag 1380
acaattgtag tacttttgtt ttaagaccaa aggccagaat agccctgatt ttataaacca 1440
gatggtaaat acagaggtac aattgtaata tttggatgca aataaagttc tatggatttg 1500
catttctact tatggtggat ctttggtcga tgcaaagtga acacacatgc agcttatgac 1560
ctgcatgcta aaggaaatat ccatttcttt ttgttaggta agggtaaact gatcccattt 1620
tcacagagaa aatgatggca taggctaaag gtttggagat gtgtcgtact tgttcttacc 1680
gtatttatct ccctcctgat ttctgttctt actgtttctt tttcttcttt tcttcctagc 1740
ttcaccaatt tgtgtttgtc tagtgaagaa taatcttggc tgtctgttgt taatcacatg 1800
gttaggaaat ttattatgtt tccataatcc gtcccttttt taatacctta aaggcaatcc 1860
cattgaatga tttgctgcca gtatattgag acatgtgaag tcttcgtcag tagattatga 1920
tgttttctaa gaaatagaca ccattgtagg tttgtgggtg acatttagct cctcctaacc 1980
tataacacaa acatatttca gattctccaa atctccagta aaactgggac cattgcattt 2040
gcaaccacaa accagctaaa gtatcaaggt gaccaagcca ctaaattgta tactaaggtt 2100
gctcacccac ccaagcactt aaaaggcagg gtggccactc gagttaatcg atacataaaa 2160
ttgattgtgg gatctggctg gcggaacctc ttcctaaaac ttatctttat ttatcaacta 2220
tgttcacttt tttctgttat ctttgtgttg agatatggga aaataatttc tagggaatat 2280
attaatttaa aattattttc tccctatgat gcagaggaag ttaattttga gttttgctca 2340
caagtatcgt cttggtcttt ggaagcccca agcagaagcg aagaaagcgc aatgaagttg 2400
agttgtcttg aagtgg 2416
<210> 19
<211> 315
<212> DNA
<213> Oryza sativa
<400> 19
atggctctcg cgtgcgcatc gcgccgcctc ctcgccggcg ccggaactcc ggcgagatcc 60
ttccactccc agccctacca agccaaggtc ggcgtggtgg agttcctcaa cggggtcggg 120
aagggggtgg aaacgcacgc cgcgaaggtg gaggaggcgg tgggaggcga cctccagagg 180
ctcctcgaga cccgcacgct gcggctcaag aagctcggca tcccctgcaa gcataggaag 240
ttaattttga gttttgctca caagtatcgt cttggtcttt ggaagcccca agcagaagcg 300
aagaaagcgc aatga 315
<210> 20
<211> 104
<212> PRT
<213> Oryza sativa
<400> 20
Met Ala Leu Ala Cys Ala Ser Arg Arg Leu Leu Ala Gly Ala Gly Thr
1 5 10 15
Pro Ala Arg Ser Phe His Ser Gln Pro Tyr Gln Ala Lys Val Gly Val
20 25 30
Val Glu Phe Leu Asn Gly Val Gly Lys Gly Val Glu Thr His Ala Ala
35 40 45
Lys Val Glu Glu Ala Val Gly Gly Asp Leu Gln Arg Leu Leu Glu Thr
50 55 60
Arg Thr Leu Arg Leu Lys Lys Leu Gly Ile Pro Cys Lys His Arg Lys
65 70 75 80
Leu Ile Leu Ser Phe Ala His Lys Tyr Arg Leu Gly Leu Trp Lys Pro
85 90 95
Gln Ala Glu Ala Lys Lys Ala Gln
100
<210> 21
<211> 710
<212> DNA
<213> Oryza sativa
<400> 21
tccacgagca atttcactac aacgatggcg gtatcttgat caagttagac tagtgtttgg 60
tttatcgcca tagttataac atgccatatt ttcttggcaa aatttgctac agaacacgca 120
aaaaatatgt aattagctgg gagacaccaa aaaacgtgac atggttgatg gccactacaa 180
aatttgtgta attggctata gaacactggt cgaattattt tattatttcc gagttccatc 240
gttttctcct tcgtcaggaa tgcgcgtggt ggggcgaggt cggcgtgagc ggggtgagcg 300
cgagctcaac ggcagcagta gggcgggtga ggaagcactg gctcgcgctg aggagcgggg 360
cgaacctatt catgcactcg ctgaagctcg atgagccgga aaagtccgtc gtcaagagct 420
ggcggaaggg cgccaaccca ctcccgcctc gctgcaccgt ggtcgtcatc cggttccgtg 480
gcaagtcccg agtcctcggc gagggcaacg gtgtggtgac tcctctgcat gtccctgcct 540
ccggttaccc gatgatgagc atggacgagc agaccagcct cagcttcgcg ctattcaaag 600
acgtggcgtt caacgccagc atccgccgcg gcgtcagggc ctccgacgtc gcttgcctgc 660
ccatctcctt cggctagtag ggcccagtgg aggaaaactg gtgactcatc 710
<210> 22
<211> 426
<212> DNA
<213> Oryza sativa
<400> 22
atggcggaat gcgcgtggtg gggcgaggtc ggcgtgagcg gggtgagcgc gagctcaacg 60
gcagcagtag ggcgggtgag gaagcactgg ctcgcgctga ggagcggggc gaacctattc 120
atgcactcgc tgaagctcga tgagccggaa aagtccgtcg tcaagagctg gcggaagggc 180
gccaacccac tcccgcctcg ctgcaccgtg gtcgtcatcc ggttccgtgg caagtcccga 240
gtcctcggcg agggcaacgg tgtggtgact cctctgcatg tccctgcctc cggttacccg 300
atgatgagca tggacgagca gaccagcctc agcttcgcgc tattcaaaga cgtggcgttc 360
aacgccagca tccgccgcgg cgtcagggcc tccgacgtcg cttgcctgcc catctccttc 420
ggctag 426
<210> 23
<211> 141
<212> PRT
<213> Oryza sativa
<400> 23
Met Ala Glu Cys Ala Trp Trp Gly Glu Val Gly Val Ser Gly Val Ser
1 5 10 15
Ala Ser Ser Thr Ala Ala Val Gly Arg Val Arg Lys His Trp Leu Ala
20 25 30
Leu Arg Ser Gly Ala Asn Leu Phe Met His Ser Leu Lys Leu Asp Glu
35 40 45
Pro Glu Lys Ser Val Val Lys Ser Trp Arg Lys Gly Ala Asn Pro Leu
50 55 60
Pro Pro Arg Cys Thr Val Val Val Ile Arg Phe Arg Gly Lys Ser Arg
65 70 75 80
Val Leu Gly Glu Gly Asn Gly Val Val Thr Pro Leu His Val Pro Ala
85 90 95
Ser Gly Tyr Pro Met Met Ser Met Asp Glu Gln Thr Ser Leu Ser Phe
100 105 110
Ala Leu Phe Lys Asp Val Ala Phe Asn Ala Ser Ile Arg Arg Gly Val
115 120 125
Arg Ala Ser Asp Val Ala Cys Leu Pro Ile Ser Phe Gly
130 135 140
<210> 24
<211> 2102
<212> DNA
<213> Oryza sativa
<400> 24
cttctccgac gatgccttcc ctccccgtcg ccgccgccga gccgatggcc gtcgacgagt 60
cggcctccaa gaagtcgaag cgcaagctga aggcggcgga ggtggaggtg gaggcctcgt 120
cgaggaagaa ggagaagaag gagaagaagc ggaaggcgaa ggagccgtcg ccatcgtcct 180
cctcctcatc cgaggaggag gagaggagca gcacgagctc cgacgagccg gcgcccgccg 240
cgaagaaggc gaagaaggag aagacgaagg agaaggtggt ggtggaggag gaggaggagg 300
acgacgacga aggggagctc accgccagcg gcgacgagga tcccgccgac cccaacgcgc 360
tcgccaactt caggatctcg gagtcgctca gggagaagct caagtccaag gggatcaagg 420
cgctgttccc catccaggcc accaccttcg atctcgtcct cgacggccac gacctggtcg 480
gccgcgcgcg cactggacag ggaaaaacat tggcttttgt tctaccgata cttgagtcat 540
tagttaatgg gacacacaag gcatccagaa ggactgatta tggcaggcct ccaactgttt 600
tggttctgtt gcctaccaga gagctagcca agcaggtgca cacagacttt gcattttatg 660
gtgcaacatt tgggctatct gcatgctgcg tatatggggg ttctgattat cgttctcaag 720
aaatggcaat cagaaagggg gttgacattg ttgttggaac tcctggtcgt gtgaaggact 780
ttgttgaaaa gggaaccctc aattttaggt ctttgaaatt ccgtgtcctt gatgaggctg 840
atgagatgct taatatgggc ttcgttgatg atgttgagct tattcttggc aaagttgaag 900
atgttacaaa agtacagaca cttctcttca gcgctactat accagagtgg gtgaagaagc 960
tctccttgag atttctcaaa tctggaaaga aaacggttga tcttgttgga gatgagaaat 1020
tgaaagctag tgcatctgtt aggcatcttg ctcttccttg taaccgtgct gcaagggctc 1080
aagttattcc agacatcatc cgatgctata gccgtggagg ccggaccatt atctttaccg 1140
agacaaaaga atctgcatca gacctctctg gtttgattgc tggatcacgt gccttgcatg 1200
gtgatgttgc tcaagctcag cgtgaagtta ttcttgctgg gttcaggagt ggcaagtttt 1260
tagttttggt tgctacaaac gtggctgcac gagggctgga cattaatgat gtgcagctta 1320
tcattcagtg tgaacctcca cgtgatgttg aagcctacat acaccggtca ggtcggactg 1380
ggagagcagg caatactggt gttgctgtca tgctttttga acccagatat aagttcaatg 1440
tgaacagaat agaaagggag tctggggtca aatttgaaca tatctctgca ccacagccaa 1500
ctgatgtggc acaatctgct ggtactgaag cagcagaagc catttccagt gtttcagaca 1560
gtgtcattcc tgtttttcgg gagcaagctg agcaactatt gaactcttcc gggatgtctg 1620
cagttgatct actagccaaa gcacttgcga aggcagttgg ctacacggac ataaagaaga 1680
gatccttgct gtcttctatg gacaaccaca ctacattact tcttcaaact ggtcgatcgg 1740
tgtatgcagc aggatttgtt ctttctactt tgaaaaggtt tatgccagaa gagagacttg 1800
cagatgtaaa gggtataacc atcactgctg atgggacagg tgctgtattt gatgttcctt 1860
cagcagaggt tgaagactat attcaaggtg cacagaacgc tgccatggtg actgtcgagg 1920
aagtgaaaca attgccaccc ttgcaagaga gggaacagtc tggtggcagc cgaggtggag 1980
gaaggtttgg aaacaggaga ttttctggtg gtggtggtgg ccggggtgga ggcggcagag 2040
ggttcggtgg aggcagaggc aggggaggtg gcggcggcaa caggtttaac aagaggtact 2100
ag 2102
<210> 25
<211> 2091
<212> DNA
<213> Oryza sativa
<400> 25
atgccttccc tccccgtcgc cgccgccgag ccgatggccg tcgacgagtc ggcctccaag 60
aagtcgaagc gcaagctgaa ggcggcggag gtggaggtgg aggcctcgtc gaggaagaag 120
gagaagaagg agaagaagcg gaaggcgaag gagccgtcgc catcgtcctc ctcctcatcc 180
gaggaggagg agaggagcag cacgagctcc gacgagccgg cgcccgccgc gaagaaggcg 240
aagaaggaga agacgaagga gaaggtggtg gtggaggagg aggaggagga cgacgacgaa 300
ggggagctca ccgccagcgg cgacgaggat cccgccgacc ccaacgcgct cgccaacttc 360
aggatctcgg agtcgctcag ggagaagctc aagtccaagg ggatcaaggc gctgttcccc 420
atccaggcca ccaccttcga tctcgtcctc gacggccacg acctggtcgg ccgcgcgcgc 480
actggacagg gaaaaacatt ggcttttgtt ctaccgatac ttgagtcatt agttaatggg 540
acacacaagg catccagaag gactgattat ggcaggcctc caactgtttt ggttctgttg 600
cctaccagag agctagccaa gcaggtgcac acagactttg cattttatgg tgcaacattt 660
gggctatctg catgctgcgt atatgggggt tctgattatc gttctcaaga aatggcaatc 720
agaaaggggg ttgacattgt tgttggaact cctggtcgtg tgaaggactt tgttgaaaag 780
ggaaccctca attttaggtc tttgaaattc cgtgtccttg atgaggctga tgagatgctt 840
aatatgggct tcgttgatga tgttgagctt attcttggca aagttgaaga tgttacaaaa 900
gtacagacac ttctcttcag cgctactata ccagagtggg tgaagaagct ctccttgaga 960
tttctcaaat ctggaaagaa aacggttgat cttgttggag atgagaaatt gaaagctagt 1020
gcatctgtta ggcatcttgc tcttccttgt aaccgtgctg caagggctca agttattcca 1080
gacatcatcc gatgctatag ccgtggaggc cggaccatta tctttaccga gacaaaagaa 1140
tctgcatcag acctctctgg tttgattgct ggatcacgtg ccttgcatgg tgatgttgct 1200
caagctcagc gtgaagttat tcttgctggg ttcaggagtg gcaagttttt agttttggtt 1260
gctacaaacg tggctgcacg agggctggac attaatgatg tgcagcttat cattcagtgt 1320
gaacctccac gtgatgttga agcctacata caccggtcag gtcggactgg gagagcaggc 1380
aatactggtg ttgctgtcat gctttttgaa cccagatata agttcaatgt gaacagaata 1440
gaaagggagt ctggggtcaa atttgaacat atctctgcac cacagccaac tgatgtggca 1500
caatctgctg gtactgaagc agcagaagcc atttccagtg tttcagacag tgtcattcct 1560
gtttttcggg agcaagctga gcaactattg aactcttccg ggatgtctgc agttgatcta 1620
ctagccaaag cacttgcgaa ggcagttggc tacacggaca taaagaagag atccttgctg 1680
tcttctatgg acaaccacac tacattactt cttcaaactg gtcgatcggt gtatgcagca 1740
ggatttgttc tttctacttt gaaaaggttt atgccagaag agagacttgc agatgtaaag 1800
ggtataacca tcactgctga tgggacaggt gctgtatttg atgttccttc agcagaggtt 1860
gaagactata ttcaaggtgc acagaacgct gccatggtga ctgtcgagga agtgaaacaa 1920
ttgccaccct tgcaagagag ggaacagtct ggtggcagcc gaggtggagg aaggtttgga 1980
aacaggagat tttctggtgg tggtggtggc cggggtggag gcggcagagg gttcggtgga 2040
ggcagaggca ggggaggtgg cggcggcaac aggtttaaca agaggtacta g 2091
<210> 26
<211> 696
<212> PRT
<213> Oryza sativa
<400> 26
Met Pro Ser Leu Pro Val Ala Ala Ala Glu Pro Met Ala Val Asp Glu
1 5 10 15
Ser Ala Ser Lys Lys Ser Lys Arg Lys Leu Lys Ala Ala Glu Val Glu
20 25 30
Val Glu Ala Ser Ser Arg Lys Lys Glu Lys Lys Glu Lys Lys Arg Lys
35 40 45
Ala Lys Glu Pro Ser Pro Ser Ser Ser Ser Ser Ser Glu Glu Glu Glu
50 55 60
Arg Ser Ser Thr Ser Ser Asp Glu Pro Ala Pro Ala Ala Lys Lys Ala
65 70 75 80
Lys Lys Glu Lys Thr Lys Glu Lys Val Val Val Glu Glu Glu Glu Glu
85 90 95
Asp Asp Asp Glu Gly Glu Leu Thr Ala Ser Gly Asp Glu Asp Pro Ala
100 105 110
Asp Pro Asn Ala Leu Ala Asn Phe Arg Ile Ser Glu Ser Leu Arg Glu
115 120 125
Lys Leu Lys Ser Lys Gly Ile Lys Ala Leu Phe Pro Ile Gln Ala Thr
130 135 140
Thr Phe Asp Leu Val Leu Asp Gly His Asp Leu Val Gly Arg Ala Arg
145 150 155 160
Thr Gly Gln Gly Lys Thr Leu Ala Phe Val Leu Pro Ile Leu Glu Ser
165 170 175
Leu Val Asn Gly Thr His Lys Ala Ser Arg Arg Thr Asp Tyr Gly Arg
180 185 190
Pro Pro Thr Val Leu Val Leu Leu Pro Thr Arg Glu Leu Ala Lys Gln
195 200 205
Val His Thr Asp Phe Ala Phe Tyr Gly Ala Thr Phe Gly Leu Ser Ala
210 215 220
Cys Cys Val Tyr Gly Gly Ser Asp Tyr Arg Ser Gln Glu Met Ala Ile
225 230 235 240
Arg Lys Gly Val Asp Ile Val Val Gly Thr Pro Gly Arg Val Lys Asp
245 250 255
Phe Val Glu Lys Gly Thr Leu Asn Phe Arg Ser Leu Lys Phe Arg Val
260 265 270
Leu Asp Glu Ala Asp Glu Met Leu Asn Met Gly Phe Val Asp Asp Val
275 280 285
Glu Leu Ile Leu Gly Lys Val Glu Asp Val Thr Lys Val Gln Thr Leu
290 295 300
Leu Phe Ser Ala Thr Ile Pro Glu Trp Val Lys Lys Leu Ser Leu Arg
305 310 315 320
Phe Leu Lys Ser Gly Lys Lys Thr Val Asp Leu Val Gly Asp Glu Lys
325 330 335
Leu Lys Ala Ser Ala Ser Val Arg His Leu Ala Leu Pro Cys Asn Arg
340 345 350
Ala Ala Arg Ala Gln Val Ile Pro Asp Ile Ile Arg Cys Tyr Ser Arg
355 360 365
Gly Gly Arg Thr Ile Ile Phe Thr Glu Thr Lys Glu Ser Ala Ser Asp
370 375 380
Leu Ser Gly Leu Ile Ala Gly Ser Arg Ala Leu His Gly Asp Val Ala
385 390 395 400
Gln Ala Gln Arg Glu Val Ile Leu Ala Gly Phe Arg Ser Gly Lys Phe
405 410 415
Leu Val Leu Val Ala Thr Asn Val Ala Ala Arg Gly Leu Asp Ile Asn
420 425 430
Asp Val Gln Leu Ile Ile Gln Cys Glu Pro Pro Arg Asp Val Glu Ala
435 440 445
Tyr Ile His Arg Ser Gly Arg Thr Gly Arg Ala Gly Asn Thr Gly Val
450 455 460
Ala Val Met Leu Phe Glu Pro Arg Tyr Lys Phe Asn Val Asn Arg Ile
465 470 475 480
Glu Arg Glu Ser Gly Val Lys Phe Glu His Ile Ser Ala Pro Gln Pro
485 490 495
Thr Asp Val Ala Gln Ser Ala Gly Thr Glu Ala Ala Glu Ala Ile Ser
500 505 510
Ser Val Ser Asp Ser Val Ile Pro Val Phe Arg Glu Gln Ala Glu Gln
515 520 525
Leu Leu Asn Ser Ser Gly Met Ser Ala Val Asp Leu Leu Ala Lys Ala
530 535 540
Leu Ala Lys Ala Val Gly Tyr Thr Asp Ile Lys Lys Arg Ser Leu Leu
545 550 555 560
Ser Ser Met Asp Asn His Thr Thr Leu Leu Leu Gln Thr Gly Arg Ser
565 570 575
Val Tyr Ala Ala Gly Phe Val Leu Ser Thr Leu Lys Arg Phe Met Pro
580 585 590
Glu Glu Arg Leu Ala Asp Val Lys Gly Ile Thr Ile Thr Ala Asp Gly
595 600 605
Thr Gly Ala Val Phe Asp Val Pro Ser Ala Glu Val Glu Asp Tyr Ile
610 615 620
Gln Gly Ala Gln Asn Ala Ala Met Val Thr Val Glu Glu Val Lys Gln
625 630 635 640
Leu Pro Pro Leu Gln Glu Arg Glu Gln Ser Gly Gly Ser Arg Gly Gly
645 650 655
Gly Arg Phe Gly Asn Arg Arg Phe Ser Gly Gly Gly Gly Gly Arg Gly
660 665 670
Gly Gly Gly Arg Gly Phe Gly Gly Gly Arg Gly Arg Gly Gly Gly Gly
675 680 685
Gly Asn Arg Phe Asn Lys Arg Tyr
690 695
<210> 27
<211> 1759
<212> DNA
<213> Oryza sativa
<400> 27
atgccgaaga tggtggtgga ggtggccgac cggtacacgg cgaggaggga cgggcggccg 60
aacaactccg gcgaggcgga cggagaggtt cgcggcgacg ttccggcgaa accgagggca 120
ggccggggtg gagggcgatg cggcgtcgcc gagggaggag acggcgacgt cggccgacgc 180
tctggcgcgg cgaccggggc agccggagga gatgtcgacg aggaggaagg tgaggccggc 240
gcgggcgacg gcgttccggc gaaattcggg cgaggaggag gtgatgccgg ggaagaaggc 300
gatgccgcgg tgtcgaagga ggagacggcg gcgtcgatcg gcgcaccggc gatgaacagc 360
agccggctgg agggagtaca gtggcaccaa tgccactggt tgccggcggg ggcaccgttc 420
cggcgagttt ccggcgagga gacggtaagg ccggtgaagg ggacggtgtt gcagagccga 480
gggaggcgac ggcacggccg gacgatgctc cggtgaggag ggagaggtgg ctggaggccg 540
gccggcgtcg gagagggaga aggagaaggc ggggagaggt gatacgacca cggggaggct 600
cgggaagggg ctaaaacgat agaacgaagg atgaggatcc cattttatag gatgggggag 660
agagccggcc acgggaggag cgggaacggc gacgggaatg gcggccggcg gcaaaggaag 720
gtggccggac ttggcgcggc cgttcccggc gattggaggc accattcaag gggaaattaa 780
gggggaattg aagaggaggg aaaggggatt aattccccct aattaatttt ggaattaacc 840
gagtgaatca agcggatttg agggaggatt ggcgctaggg ttcgcacggg gagagggaag 900
ccgggcggcg gctggaggaa gaaggcgacg gcggcctgac gcgtgggccc cacgcggggc 960
gcgcggtcag cgcgcgcacg tgcgcgctcg ggcgggaggg cgccgctcga gttgggccgg 1020
gcggccggcc caggaggggg gggggagggg taggcggctt gggctgggcg gctgggccga 1080
ggcaggccgg gaggaaggag ggggggaagg gagctgggcc gagaggggag gaggcccaag 1140
aggagggaga gaaggagagg gagagaaaga gagggaggag ggaaggactt cgggctgggc 1200
ttggcccaaa ggaggaagag gagggatttt tagtttttct ttttaataaa ctttgatcaa 1260
ggttgttgtt gcttaataat tatttccggt gctctgaaaa ttcaagtaaa atttgagggc 1320
tctttttaga ccaaggagaa tttaacaaaa attctccggg ccacattgga atttttcttg 1380
cacgtatttt agtgtttgcc aatttctttt cgaattttaa ttaattctat tattcctttt 1440
agcgaatgaa ttttatttcg ggatgaattt atcaggacgt gacaaatcca ccccccttac 1500
aagaatctcg tccccgagat tcgaggaggc tagcaagaag ataggttggg cggtcttctc 1560
ttcttgtctg acttcttccg ctccgtcttc tataacaaac ttgcctagca aaacttctcc 1620
actctggact gcttggctca atctcctgtt gcagatggaa tcttctcttc acctgacttt 1680
tccctggttg tacctctagt tgacttcgac tggattatgg cacttgaaga tgggactttc 1740
gttccagaac ttctcataa 1759
<210> 28
<211> 504
<212> DNA
<213> Oryza sativa
<400> 28
atgccgaaga tggtggtgga ggtggccgac cggtacacgg cgaggaggga cgggcggccg 60
aacaactccg gcgaggcgga cggagaggtt cgcggcgacg ttccggcgaa accgagggca 120
ggccggggtg gagggcgatg cggcgtcgcc gagggaggag acggcgacgt cggccgacgc 180
tctggcgcgg cgaccggggc agccggagga gatgtcgacg aggaggaagg tgaggccggc 240
gcgggcgacg gcgttccggc gaaattcggg cgaggaggag gtgatgccgg ggaagaaggc 300
gatgccgcgg tgtcgaagga ggagacggcg gcgtcgatcg gcgcaccggc gatgaacagc 360
agccggctgg agggagtaca gtggcaccaa tgccactggt tgccggcggg ggcaccgttc 420
cggcgagttt ccggcgagga gacgttgact tcgactggat tatggcactt gaagatggga 480
ctttcgttcc agaacttctc ataa 504
<210> 29
<211> 167
<212> PRT
<213> Oryza sativa
<400> 29
Met Pro Lys Met Val Val Glu Val Ala Asp Arg Tyr Thr Ala Arg Arg
1 5 10 15
Asp Gly Arg Pro Asn Asn Ser Gly Glu Ala Asp Gly Glu Val Arg Gly
20 25 30
Asp Val Pro Ala Lys Pro Arg Ala Gly Arg Gly Gly Gly Arg Cys Gly
35 40 45
Val Ala Glu Gly Gly Asp Gly Asp Val Gly Arg Arg Ser Gly Ala Ala
50 55 60
Thr Gly Ala Ala Gly Gly Asp Val Asp Glu Glu Glu Gly Glu Ala Gly
65 70 75 80
Ala Gly Asp Gly Val Pro Ala Lys Phe Gly Arg Gly Gly Gly Asp Ala
85 90 95
Gly Glu Glu Gly Asp Ala Ala Val Ser Lys Glu Glu Thr Ala Ala Ser
100 105 110
Ile Gly Ala Pro Ala Met Asn Ser Ser Arg Leu Glu Gly Val Gln Trp
115 120 125
His Gln Cys His Trp Leu Pro Ala Gly Ala Pro Phe Arg Arg Val Ser
130 135 140
Gly Glu Glu Thr Leu Thr Ser Thr Gly Leu Trp His Leu Lys Met Gly
145 150 155 160
Leu Ser Phe Gln Asn Phe Ser
165
<210> 30
<211> 428
<212> DNA
<213> Oryza sativa
<400> 30
ggcgggcgat gcagatccgc gtgcggtgcg ggtgcggcga ggcggggtgc ccggagtggg 60
ccatcgtgga ggtgcagggc gtggtgcagc cacagccgtg cttctccggc cgcatccagg 120
gcctccacat cggccgcctc tgcgccgccg ccgccccgtc ttccaaggcg gcgttcacct 180
tcaccgtggg gtaccatgag ctcgccggca ccaaggtggc gctcaagaag cctctcctcg 240
tgctgaggaa gaagaagacg acggcggtgg cggcggagac ggagctggag gtgatcggcg 300
tgatccggca caagatcctc ttcaaggacc ggcccaaggc tctcatctca aaaccgcagg 360
tgaaggagaa gaagactctg ccgctaccgg cgccggcggc ggcgccacca ccccaatcat 420
cgtagcac 428
<210> 31
<211> 417
<212> DNA
<213> Oryza sativa
<400> 31
atgcagatcc gcgtgcggtg cgggtgcggc gaggcggggt gcccggagtg ggccatcgtg 60
gaggtgcagg gcgtggtgca gccacagccg tgcttctccg gccgcatcca gggcctccac 120
atcggccgcc tctgcgccgc cgccgccccg tcttccaagg cggcgttcac cttcaccgtg 180
gggtaccatg agctcgccgg caccaaggtg gcgctcaaga agcctctcct cgtgctgagg 240
aagaagaaga cgacggcggt ggcggcggag acggagctgg aggtgatcgg cgtgatccgg 300
cacaagatcc tcttcaagga ccggcccaag gctctcatct caaaaccgca ggtgaaggag 360
aagaagactc tgccgctacc ggcgccggcg gcggcgccac caccccaatc atcgtag 417
<210> 32
<211> 138
<212> PRT
<213> Oryza sativa
<400> 32
Met Gln Ile Arg Val Arg Cys Gly Cys Gly Glu Ala Gly Cys Pro Glu
1 5 10 15
Trp Ala Ile Val Glu Val Gln Gly Val Val Gln Pro Gln Pro Cys Phe
20 25 30
Ser Gly Arg Ile Gln Gly Leu His Ile Gly Arg Leu Cys Ala Ala Ala
35 40 45
Ala Pro Ser Ser Lys Ala Ala Phe Thr Phe Thr Val Gly Tyr His Glu
50 55 60
Leu Ala Gly Thr Lys Val Ala Leu Lys Lys Pro Leu Leu Val Leu Arg
65 70 75 80
Lys Lys Lys Thr Thr Ala Val Ala Ala Glu Thr Glu Leu Glu Val Ile
85 90 95
Gly Val Ile Arg His Lys Ile Leu Phe Lys Asp Arg Pro Lys Ala Leu
100 105 110
Ile Ser Lys Pro Gln Val Lys Glu Lys Lys Thr Leu Pro Leu Pro Ala
115 120 125
Pro Ala Ala Ala Pro Pro Pro Gln Ser Ser
130 135
<210> 33
<211> 3764
<212> DNA
<213> Oryza sativa
<400> 33
catccgtgcc gagcgagtcc aatggcctct aacctcaacc gacctgattt gacatcttgg 60
acctggcgtt cgatggatga ggtgacaccg ggttcgatgg ggaaggagga gacgctggcc 120
ccgggaggga gctcggggga ggacgccgct gcatcctcta ttcctctgtg agagctcgag 180
ctcgggaggt gaagccgggg aggaagtggg gtcgcaagga tggaagcggg atcaaggggt 240
catcattagg agggcggagg cggagggggt ggcgtggttt ctgccgctgt tttgatgctg 300
cgggagtcgg tatgtgcgga tgcggatgga tagtagagaa ttaatctgtg atttggcctg 360
taggattgct acagatgaac tcctcctttt tatattagta taggaatata actagagatg 420
attcggcgat tatgctttga atcatgattt tttttataga tattacggga tctattcaga 480
acactctttt gtgagaagtt cgaaccatcc acttaggaat tatactacaa tacaaacaca 540
ctgtggtttg aatttttttc aaaactgcag attagtagtg aactcatcat tgtcttcaaa 600
taaccaagct tgtgtccatt gaattagcat aatttgcctc tcttaataaa acagtaggga 660
tcaataaaat aaagcagtgg tcaaagttgc attttggtac tgttaatgtc aaaatcgata 720
tgttcactag accagagtag tataaattct gccattatgt ctttacgtgg aacactttga 780
gatctgtatg cttagtgtat taatgatttt gcacatatct atctaataaa ttatctgcat 840
gaccattgca gctagctagc cagctatgca tgtcctctac catgactaga gagaccggtt 900
ggcaaaaggg cataatgcac tccagtgttc taaactgttt ctttatcata tttctgcatt 960
aataaaaaaa taacatttcg gtgcgcagcg gtgaccatga taccgttttg ccatttcttg 1020
gtacacaaac ctgggtgagg tcactcaact atcccattgt agatgactgg agagcttggc 1080
atgtggatgg ccagtccgca gggtgagttt taattatgtt tgattagttc tgttgtttgg 1140
cttacataat caaaatggtt gtctaataag ttttcagtat acaaactgct ttcaggaata 1200
acttttctaa aacttcaatt aatttgcaga ttcaccgtag catatggaaa caacctgaca 1260
tttgctactg ttaaggtaac aacccatcaa tgcacatcca attttatgaa taatttatat 1320
atagatattt ccaaaggttg ttgtgaatgg agttacgttc tgattcacga ttacatatcg 1380
ttgatacaaa ttcaacccgc gtcatgatga ggatttttaa tctgtattac tgctaatgat 1440
atttacttta gttcaaagat tattctattt tatcaaaaat ttcttatatt atttaaaaat 1500
gaaatcattg gtatgcgaag aaatcctcac aacatgggaa agaccttgcc acaccagtgt 1560
tagcaacatt tgtggcccca aggttcattt tttaaatata agttcattga aggttcattt 1620
tttaatttat attagaaagg ttaaaagata ataaaatttc aaaaactctt tagaaggacc 1680
ttgatatcac caaggggtca aattggcgtt cctaaacatt cgatttgaat ccggaacatt 1740
tggtctaaga ttaagagttg tgatcttata gcaaaacgtc caatggcaag gtggtaaatc 1800
aagaactaac cacttgcaga tcaaacaaac aacaaatata gctaccaaaa tgtgtgaaac 1860
aagatacaaa gaaaataaca atatcatcaa ctagattaaa ctaagcacat aggtagatga 1920
ggcaaatgaa gaataataat ggaaaaacac acgagagaca aaatcaaaat atttgttctg 1980
tgatgtttag atccactgat tccatttcaa aggccatgtc tctttccact cttatactca 2040
ctaaggcaac accaaagtgc ttctgatgtt tagtctttat ttcttctatt atggaggcaa 2100
aatcgaaatc ttgacaaaca tccatgccac actacataag cttgggtact caccagcaac 2160
acccagccat ctaaaagacc ttaatgtcca atatagtaat acacacatta caaaatttcc 2220
tataaactca agtgctcaga cgatgaagta aactcactag cttacaacat ttcaatctgt 2280
cactaatttg ataaaaaaaa gaggggaggg gggggatatg taagaactat atggtcatgg 2340
atatgcataa ctctttgtac aaactcaaca actcctaaag gagggggatg agagctttaa 2400
atagcatcgt tatatttcta cccgttaagg taaaaacaat aggtggctga attaaaataa 2460
ccataacatc tcgctctcaa gtctgttttc gaaatcttga actctatgta aagcttaatt 2520
cgacatctag gtaacacaac taaaaccaca caaagtagat gttaaaactg ttgattctct 2580
taagatagca cttggatatg tcactttgag caccgtcaat aatcaaggta caattctgca 2640
ttcctcttga caggacataa ttcctaaact caaatgcaat tagaaaaata actataccaa 2700
tgagaacaca tgtactgtca tggttatgga taccaaatac ctaatagtag ttgattaggc 2760
acggctggac ccaccacata tcaagtctta tacggaatta ggcctcactt acggaaggta 2820
ttccggataa ggaaggacaa gtaaagttct atatggaaac tataaggact acttggattg 2880
tatccatatt ggtctcccta gttctagttg gataagggga cacctatagg cataaataga 2940
ccccctagga ggagagggag ataagccaca agacatacaa atcgacacac aagccaggac 3000
aaaccaatac gccaccagat atcgacatca gagataagcc tagacagacc ccatttggtg 3060
cctgtagagg ccaactaaag ggatctaatg ccatctttga tctcgatgag ttcatattcg 3120
aggaggaaga ctaccctatc gtcgactacg tgttagtgat ccatgccgcc aagtcgatga 3180
cagctagata ggttttccca aatattgtac ttgtgtgatt cagataaata agagcaacat 3240
cgacttcggc caacaggagt agggctatta cctgtcagat aaggggtccg aacctatata 3300
aaatccttgt tttcatctct tttacctcaa tctcgcatat accctggtac caacgatccc 3360
cataccatcc aaatacccta gccgagacct aaaaggtcga catgtacgtc atcttctttc 3420
cttatcatga ggattgcctt gatgaattat cactttcgat tttagctaga tattcatcaa 3480
tatatgaagt ttgaccactt ctatatcttc atgccacttg atgtcattca acaagtaaag 3540
tccaatcttt acccattttg cagtcaaact cgatgtgggt ccacctatga attacatctt 3600
attgatcttc accatttcat cttgcattct tttaatggtt gatgtgggtt actcatagat 3660
tcaactgacc tcgggtggac catcacggca aagccttcaa tcatccttat gaatgaaatg 3720
gtcacggaaa ttacctactg acctcgtttc tgaccattac cgtt 3764
<210> 34
<211> 486
<212> DNA
<213> Oryza sativa
<400> 34
atggcctcta acctcaaccg acctgatttg acatcttgga cctggcgttc gatggatgag 60
gtgacaccgg gttcgatggg gaaggaggag acgctggccc cgggagggag ctcgggggag 120
gacgccgctg catcctctat tcctctcggt gaccatgata ccgttttgcc atttcttggt 180
acacaaacct gggtgaggtc actcaactat cccattgtag atgactggag agcttggcat 240
gtggatggcc agtccgcagg attcaccgta gcatatggaa acaacctgac atttgctact 300
gttaaggtaa cacaactaaa accacacaaa gtagatgtta aaactgttga ttctcttaag 360
atagcacttg gatatgtcac tttgagcacc gtcaataatc aagattcaac tgacctcggg 420
tggaccatca cggcaaagcc ttcaatcatc cttatgaatg aaatggtcac ggaaattacc 480
tactga 486
<210> 35
<211> 161
<212> PRT
<213> Oryza sativa
<400> 35
Met Ala Ser Asn Leu Asn Arg Pro Asp Leu Thr Ser Trp Thr Trp Arg
1 5 10 15
Ser Met Asp Glu Val Thr Pro Gly Ser Met Gly Lys Glu Glu Thr Leu
20 25 30
Ala Pro Gly Gly Ser Ser Gly Glu Asp Ala Ala Ala Ser Ser Ile Pro
35 40 45
Leu Gly Asp His Asp Thr Val Leu Pro Phe Leu Gly Thr Gln Thr Trp
50 55 60
Val Arg Ser Leu Asn Tyr Pro Ile Val Asp Asp Trp Arg Ala Trp His
65 70 75 80
Val Asp Gly Gln Ser Ala Gly Phe Thr Val Ala Tyr Gly Asn Asn Leu
85 90 95
Thr Phe Ala Thr Val Lys Val Thr Gln Leu Lys Pro His Lys Val Asp
100 105 110
Val Lys Thr Val Asp Ser Leu Lys Ile Ala Leu Gly Tyr Val Thr Leu
115 120 125
Ser Thr Val Asn Asn Gln Asp Ser Thr Asp Leu Gly Trp Thr Ile Thr
130 135 140
Ala Lys Pro Ser Ile Ile Leu Met Asn Glu Met Val Thr Glu Ile Thr
145 150 155 160
Tyr
<210> 36
<211> 2729
<212> DNA
<213> Oryza sativa
<400> 36
ctctcatgct cggcgctttc tctcccacgt gtctcttcct ctcccccagc cagatctggt 60
cctgccgctg ccaaagctga ccggcgtttg cgcggtagcc aggacggtgg cggaggggaa 120
gtgggaggca gaggtgcggc gatggcggcc agaggcagcc ggtcctgcgc ctggccgtgt 180
gggttgggga tggctggagg tggtcaggag ggcagcggga accggcaagg agtcggcgat 240
ggagtccctc cggacggcgg tagggacagc gaagcacggc ggaagatggt gacggcgctc 300
ggcggctacg gcagccctag gggctgcggc gtcccaagac agtggcagtc tgagcactgt 360
gaatccgata aagtggaagc tgatcccatc ggttcgtgta tggttggcta gtttcagcta 420
aacgacgaat gacgacgcat gaaagtgggc taactggcgg catgtggcgt cagcttatct 480
gaaatggata aagatggcac caacggaggt ttacattgac ggcgcactag gggcggcggc 540
gactagacga ctttcactcg tcaaatcggg ctgtttcagg agaacaaggt gggacgtaga 600
gaaacaagga tgtgtcgagg ttgtatggtt ttcttttcgt tttttggttg tgtgttttcc 660
tccttgttga ggtgtgagtc taaatactca cgtatcattt tggttgtgta tattattcgt 720
ggatatagag gccagattaa taaaaattta ttataaaaaa aatcctaacc caaactgccg 780
aaaacggctt tagggtgtgt ttgaggagaa ggggattgag gagattggga agataagcaa 840
aacgaggtga gccattaact catgattaat tgagtattaa ctattttaaa tttaaaaaat 900
ggattaatat gattttttta aagcaacttt catatagaaa atttttgcaa aaaacacacc 960
gtttagtagt ttgggaagcg tgcgcgtgga aaacgatgtg cattctcact ccctatcatc 1020
caaacgaacg cagccttaga gcatctccaa caggatggct atttttggct ctccatttgt 1080
ctatttagcc aactaaccat aaagattcct ctcccaatct gcgcaaagac tctctaaatc 1140
ctaatggcta gtttcctctc aaaaaatcca tatattgcat ttagttcaca cataccatat 1200
ccatatattg cattggtcca tacattgcat tgcattcagt tcactaaatt aaaggtccat 1260
acattgcatt gcattcagtt cactaaatta aaggttcata cattgcatta caaaagttca 1320
ctaatttaaa agtccataca ctacactgca tacattgcat tgcaatcagt ccagtaattt 1380
aaaggtccat acattctatt acaaaagtca aataatttaa aggtccatgc attgcattgc 1440
aattagtcca ctgatttaaa ggtccatcat attgcactgc atttagtccg tagcagtaca 1500
caaaagttta gaggtgatta attttgatgc aaataaagat accccacttg gaatccttaa 1560
accgcttacc aaggatttca gcttgcaatg agtcatagta cattttcctt ggtccagtca 1620
agcaaaataa tgcagttcat acaaaatcaa tattgtgagc accatgcttc tccacgcact 1680
tgcaaacgca aacactataa caacaaggta catttgatgc acacatattt ctttcgatct 1740
tcagagttgc acacattaca gagcaaatac acattacagt tgcacacata tttctttcga 1800
tctttcgatc tacagagcaa atacagaggg gattttcgat ctacagagcc aatacagagg 1860
ggaaaggagt ggtggatggg ggttttacct gctttgctat gggcatcggc agggtggtct 1920
cagcagggac ggcggcggcc tcagcaggtc ctatcggcag cagtcaatgg cggcttcggc 1980
aacaaaagtg aaaagacgac ggcggagggg cggccctggt agcagaagac gacgacggag 2040
gggtggcgca gaggggcggc gctggcggca aaagacaacg gcggtggtcg acggcgtctt 2100
ggcaacaaaa cacaacggca gaggggcgcc cctggccgag aggacgccgg cggaggggtg 2160
gcgcacaggg gcggcaccgg cggagagatg cgcgcgctgg atctggggga agggaaaaac 2220
gcgatctgtg gaggagagga taggtgggag tagtacacgt gcgtgggccc acgaatggct 2280
aggccttttt ggctggccaa atttggccag tgcgagcagc actttgtcca gccggatggc 2340
ttggcttggc cagtttgttg gaggacaaat tttaattaaa attgtcaaaa tttaatttag 2400
agagtagact aaggaggctg ttggagatgc tcttagtagt agtatcgaac atcgggacat 2460
accaaacgag agggattttg gtcaaggatt tactaaagta gtagtagatc atacgcacac 2520
gtatataaac agatccatga atccatatcc atgcatgcat acatgcaccg tcatcaggat 2580
attccaactc ttttggagac agggtacgtg acagggcccg aaagcgatgt gcctgccctt 2640
ttttaggctc cggccggaac tactagcgta gccatgcacc ctcgtaaccg agcgacgaga 2700
tatatcgatc gcgatgtgga tggtgccgg 2729
<210> 37
<211> 483
<212> DNA
<213> Oryza sativa
<400> 37
atggcggcca gaggcagccg gtcctgcgcc tggccgtgtg ggttggggat ggctggaggt 60
ggtcaggagg gcagcgggaa ccggcaagga gtcggcgatg gagtccctcc ggacggcggt 120
agggacagcg aagcacggcg gaagatggtg acggcgctcg gcggctacgg cagccctagg 180
ggctgcggcg tcccaagaca gtggcagtct gagcactgtg aatccgataa agtggaagct 240
gatcccatcg agcaaataca gaggggattt tcgatctaca gagccaatac agaggggaaa 300
ggagtggtgg atgggggttt tacctgcttt gctatgggca tcggcagggt ggtctcagca 360
gggacggcgg cggcctcagc agagagtaga ctaaggaggc tgttggagat gctcttagta 420
gtagtatcga acatcgggac ataccaaacg agagggattt tggtcaagga tttactaaag 480
tag 483
<210> 38
<211> 160
<212> PRT
<213> Oryza sativa
<400> 38
Met Ala Ala Arg Gly Ser Arg Ser Cys Ala Trp Pro Cys Gly Leu Gly
1 5 10 15
Met Ala Gly Gly Gly Gln Glu Gly Ser Gly Asn Arg Gln Gly Val Gly
20 25 30
Asp Gly Val Pro Pro Asp Gly Gly Arg Asp Ser Glu Ala Arg Arg Lys
35 40 45
Met Val Thr Ala Leu Gly Gly Tyr Gly Ser Pro Arg Gly Cys Gly Val
50 55 60
Pro Arg Gln Trp Gln Ser Glu His Cys Glu Ser Asp Lys Val Glu Ala
65 70 75 80
Asp Pro Ile Glu Gln Ile Gln Arg Gly Phe Ser Ile Tyr Arg Ala Asn
85 90 95
Thr Glu Gly Lys Gly Val Val Asp Gly Gly Phe Thr Cys Phe Ala Met
100 105 110
Gly Ile Gly Arg Val Val Ser Ala Gly Thr Ala Ala Ala Ser Ala Glu
115 120 125
Ser Arg Leu Arg Arg Leu Leu Glu Met Leu Leu Val Val Val Ser Asn
130 135 140
Ile Gly Thr Tyr Gln Thr Arg Gly Ile Leu Val Lys Asp Leu Leu Lys
145 150 155 160
<210> 39
<211> 24
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning gDNA of OsFBX335 gene
<400> 39
ggcgcgaatt ccggcgaggt attc 24
<210> 40
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning gDNA of OsFBX335 gene
<400> 40
gaaagcaccg gacactttaa aatcac 26
<210> 41
<211> 24
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning gDNA of OsNADH1 gene
<400> 41
ccatgttacg agcttctgga atac 24
<210> 42
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning gDNA of OsNADH1 gene
<400> 42
ggcctagtgg ggtctctctg gtg 23
<210> 43
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning cDNA of OsPUF1 gene
<400> 43
ctgctgaggc ttcccgggcc tcttcaaaaa tcc 33
<210> 44
<211> 36
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning cDNA of OsPUF1 gene
<400> 44
ccgctgaggg aaacacccaa atcatcggtt ccagtg 36
<210> 45
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning cDNA of OsPK1 gene
<400> 45
ctgctgaggc tcgctctcca ttcccaaatc tcctc 35
<210> 46
<211> 36
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning cDNA of OsPK1 gene
<400> 46
ccgctgaggg tcttcacatc aaaccttcct gacctg 36
<210> 47
<211> 38
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning gDNA of OsFAO1 gene
<400> 47
ctgctgaggg aagacgacga ctcactgcta gctggtag 38
<210> 48
<211> 36
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning gDNA of OsFAO1 gene
<400> 48
ccgctgaggc ataaacagtc cccacaacgc ttagtc 36
<210> 49
<211> 34
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning gDNA of OsDN-DTP17 gene
<400> 49
ctgctgaggg tctctctcca gcttctctct cccc 34
<210> 50
<211> 36
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning gDNA of OsDN-DTP17 gene
<400> 50
ccgctgaggc cacttcaaga caactcaact tcattg 36
<210> 51
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning gDNA of OsCuAO1 gene
<400> 51
ctgctgaggt ccacgagcaa tttcactaca acgat 35
<210> 52
<211> 34
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning gDNA of OsCuAO1 gene
<400> 52
ccgctgaggg atgagtcacc agttttcctc cact 34
<210> 53
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning cDNA of OsDRH7 gene
<400> 53
cttctccgac gatgccttcc ctc 23
<210> 54
<211> 29
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning cDNA of OsDRH7 gene
<400> 54
ctagtacctc ttgttaaacc tgttgccgc 29
<210> 55
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning gDNA of OsDN-DTP9 gene
<400> 55
ctgctgagga tgccgaagat ggtggtggag 30
<210> 56
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning gDNA of OsDN-DTP9 gene
<400> 56
ccgctgaggt tatgagaagt tctggaacga aagtc 35
<210> 57
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning cDNA of OsDN-DTP18 gene
<400> 57
ctgctgaggg gcgggcgatg cagatcc 27
<210> 58
<211> 29
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning cDNA of OsDN-DTP18 gene
<400> 58
ccgctgaggg tgctacgatg attggggtg 29
<210> 59
<211> 24
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning gDNA of OsSCP52 gene
<400> 59
catccgtgcc gagcgagtcc aatg 24
<210> 60
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning gDNA of OsSCP52 gene
<400> 60
aacggtaatg gtcagaaacg aggtcag 27
<210> 61
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for cloning gDNA of OsDN-DTP19 gene
<400> 61
ctgctgaggc tctcatgctc ggcgctttct ctc 33
<210> 62
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for cloning gDNA of OsDN-DTP19 gene
<400> 62
ccgctgaggc cggcaccatc cacatcgcga tc 32
<210> 63
<211> 21
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for real-time PCR analysis of OsFBX335 gene
<400> 63
acgacagatg ctttcctatg g 21
<210> 64
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for real-time PCR analysis of OsFBX335 gene
<400> 64
tttagcttga ccgtggaagg 20
<210> 65
<211> 21
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for real-time PCR analysis of OsPK1 gene
<400> 65
ggaggttgca cttgttcaaa g 21
<210> 66
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for real-time PCR analysis of OsPK1 gene
<400> 66
ctgacctgaa tattatgggt gga 23
<210> 67
<211> 22
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer for real-time PCR analysis of OsDRH7 gene
<400> 67
agagacttgc agatgtaaag gg 22
<210> 68
<211> 21
<212> DNA
<213> Artificial Sequence
<220>
<223> Reverse primer for real-time PCR analysis of OsDRH7 gene
<400> 68
aacctctgct gaaggaacat c 21
<210> 69
<211> 1533
<212> DNA
<213> Oryza sativa
<400> 69
atggggaagc tgctggacct gaagcggctc atgtccgtgc cgaaggagcg gaatcgtcgt 60
cggcggcagc ggcaaatccg agcccgcaat ggttcaattg attcagtggc taaaagaaag 120
ggatcactct gccaacaagt tgcaaattct gatggcgaga gaagaacaag atattcaggg 180
cctaaccttc cagaggacat ctggtgccat atacactccc tgatgccatt caaagatgct 240
gcccgtgctg catgtgtgtc tcatgctttt cgtcgttcct ggcaacaccg ccccaatctt 300
atattttgta ttggaacact ggggttggat ttcatcaaca aaattgatcg tattataaaa 360
aaccactcag gcattggcat caaatcgcta cagttagaat atgatacttt ttgcaatgct 420
agaaggagcg ctagcatcag ctatcatctc aataattggc ttcagattgc tgttacacca 480
tggattgaag aactaatcct tacactgtcg ctatcttcat acaatgtgga ttacaatttc 540
ccgtgctcac ttttatctga tgggagagga agttcattgc gacatcttta tcttggcagt 600
tgtttctttc atcctacagt taatcttgaa ttgagaaacc tgacgaggtt gcatctggtt 660
actgtacata ttacaggaga tgagtttggg tgccttcttt cgaattctta tgctttggag 720
cgattggaac ttaaatattg ttatggaata atttgtctga agataccttg cctgttgcaa 780
cgccttagcc acctggaagt gtttgaatgc agaatgctgc aagccataga gaataaggct 840
ccaaatcttt gcagttttga ccttggagct aggcaggtac ggctcttgct tggtgaatca 900
ttgcaaatga aaacactatc cttggactat cctagtgccg tctattatgc tcgtgcagag 960
cttccatcca atgtgccaaa tcttgaaatt ctcaccattt gctcggatca cgagatggtt 1020
gatacaccaa tgttgcctag caaatttttc tatctcaagt gcttgactat tgatcttgct 1080
tggagacttt ccccagccta tgattatttc tctctgattt cttttcttga cgcttctcct 1140
tccttggaga ctttttgtct agaggaccga atggagaatg aattgattat tggagatatg 1200
tcacatatga ggcaaatgct agaacaccgt catgacaacc tccagagcgt ggagattatt 1260
ggcttttgct atacaaagag cttgatcgag ctaacatgtc atattcttga caatacaacg 1320
tcgcttaagc accttaaact ggatactact cgtgatgttt ttagctgttc tacaggcaaa 1380
cacgacaaat gcttccatat gggtaaggat atgctcaccg aagccaagaa agcagttttg 1440
gcaattgaaa cttacatcga gccaaaagtt ccctccactg tcatgctaaa tgttgtgaaa 1500
ccttgcaacc gttgccatgt tgctgaatct tag 1533
<210> 70
<211> 510
<212> PRT
<213> Oryza sativa
<400> 70
Met Gly Lys Leu Leu Asp Leu Lys Arg Leu Met Ser Val Pro Lys Glu
1 5 10 15
Arg Asn Arg Arg Arg Arg Gln Arg Gln Ile Arg Ala Arg Asn Gly Ser
20 25 30
Ile Asp Ser Val Ala Lys Arg Lys Gly Ser Leu Cys Gln Gln Val Ala
35 40 45
Asn Ser Asp Gly Glu Arg Arg Thr Arg Tyr Ser Gly Pro Asn Leu Pro
50 55 60
Glu Asp Ile Trp Cys His Ile His Ser Leu Met Pro Phe Lys Asp Ala
65 70 75 80
Ala Arg Ala Ala Cys Val Ser His Ala Phe Arg Arg Ser Trp Gln His
85 90 95
Arg Pro Asn Leu Ile Phe Cys Ile Gly Thr Leu Gly Leu Asp Phe Ile
100 105 110
Asn Lys Ile Asp Arg Ile Ile Lys Asn His Ser Gly Ile Gly Ile Lys
115 120 125
Ser Leu Gln Leu Glu Tyr Asp Thr Phe Cys Asn Ala Arg Arg Ser Ala
130 135 140
Ser Ile Ser Tyr His Leu Asn Asn Trp Leu Gln Ile Ala Val Thr Pro
145 150 155 160
Trp Ile Glu Glu Leu Ile Leu Thr Leu Ser Leu Ser Ser Tyr Asn Val
165 170 175
Asp Tyr Asn Phe Pro Cys Ser Leu Leu Ser Asp Gly Arg Gly Ser Ser
180 185 190
Leu Arg His Leu Tyr Leu Gly Ser Cys Phe Phe His Pro Thr Val Asn
195 200 205
Leu Glu Leu Arg Asn Leu Thr Arg Leu His Leu Val Thr Val His Ile
210 215 220
Thr Gly Asp Glu Phe Gly Cys Leu Leu Ser Asn Ser Tyr Ala Leu Glu
225 230 235 240
Arg Leu Glu Leu Lys Tyr Cys Tyr Gly Ile Ile Cys Leu Lys Ile Pro
245 250 255
Cys Leu Leu Gln Arg Leu Ser His Leu Glu Val Phe Glu Cys Arg Met
260 265 270
Leu Gln Ala Ile Glu Asn Lys Ala Pro Asn Leu Cys Ser Phe Asp Leu
275 280 285
Gly Ala Arg Gln Val Arg Leu Leu Leu Gly Glu Ser Leu Gln Met Lys
290 295 300
Thr Leu Ser Leu Asp Tyr Pro Ser Ala Val Tyr Tyr Ala Arg Ala Glu
305 310 315 320
Leu Pro Ser Asn Val Pro Asn Leu Glu Ile Leu Thr Ile Cys Ser Asp
325 330 335
His Glu Met Val Asp Thr Pro Met Leu Pro Ser Lys Phe Phe Tyr Leu
340 345 350
Lys Cys Leu Thr Ile Asp Leu Ala Trp Arg Leu Ser Pro Ala Tyr Asp
355 360 365
Tyr Phe Ser Leu Ile Ser Phe Leu Asp Ala Ser Pro Ser Leu Glu Thr
370 375 380
Phe Cys Leu Glu Asp Arg Met Glu Asn Glu Leu Ile Ile Gly Asp Met
385 390 395 400
Ser His Met Arg Gln Met Leu Glu His Arg His Asp Asn Leu Gln Ser
405 410 415
Val Glu Ile Ile Gly Phe Cys Tyr Thr Lys Ser Leu Ile Glu Leu Thr
420 425 430
Cys His Ile Leu Asp Asn Thr Thr Ser Leu Lys His Leu Lys Leu Asp
435 440 445
Thr Thr Arg Asp Val Phe Ser Cys Ser Thr Gly Lys His Asp Lys Cys
450 455 460
Phe His Met Gly Lys Asp Met Leu Thr Glu Ala Lys Lys Ala Val Leu
465 470 475 480
Ala Ile Glu Thr Tyr Ile Glu Pro Lys Val Pro Ser Thr Val Met Leu
485 490 495
Asn Val Val Lys Pro Cys Asn Arg Cys His Val Ala Glu Ser
500 505 510
<210> 71
<211> 1770
<212> DNA
<213> Zea mays
<400> 71
atgccgctgc gcgactgcaa cgtactgatc aggaccctgg ccaggcgtgg cagtttctca 60
cgcgtcatgg cggtgtatta cgatctccgt gcgcgaggcc tcgccgcaga cagctacacc 120
tacccatttg tgctcatggc aatcagcacg atgaagctgt ccgtcgaggg gcgcaaggtg 180
cacgcggccg cggtgaagac cggcttccgc tgggatgcgt acaccacatg ttcgctaatg 240
gagatgtaca cgatgctggg ccggagggtg ttcgacaaga tgccgcagcg attcctggtg 300
ctgtggaata tgatgatgag ctacaaggaa aagaggtctt gccagcacaa tgatgattct 360
aaaggggaac aagaaaaacc attcctttca gagggtgtct ggtctcatat acattccttt 420
atgcacattc gagacgcggg gcgagctgcc tgtttgtccc ggtcttttct acagtcatgg 480
agatgtcatc cctacctcat ttttactgat gaaacattgg gttgcaagaa aaacacatct 540
caagaaaacg aaattgcaag caatttttac agtagagttg ataacattct gaataatcat 600
tcaggcattg ggatgaaggc actcaaaatc catgttccta taggttacac tgcaaaggac 660
tcacgttatc ttaaccgttg gcttcagatt gctatcacac atgggattga agaactcgag 720
ctaatttttc ctttcatggc aaagtacaaa ttcccatatt cccttctacc tatgagtgga 780
gactcaatca aatgtcttga acttagttgc tgttcttttt gtcccacttc taagtttggc 840
tggttaaaaa atctgacaaa attgtgtctt gatggtgttt ttattgaggg tgacgagtta 900
gagcgccttg tcttctctgc ccttgctttg gagaggttgg agattagata ttgtgatcgc 960
atagtctgtc tgaaggttcc ttccatgcta caacggctca catacctaga ggtttatgat 1020
tgtgacaagc ttcgtgtttt ggacatcgat gctccaaata taacttgctt caatttcgga 1080
agacatcata cgaagacgaa actgtcaatt ggtgggggag tgtccaaaat aaaagaattg 1140
agtgcatgct tcgacgatgc agtttattat gtccgtgttg aactcccgtc tatgatggca 1200
aacctcgaaa ctcttaccat aaattcaatg gttgagatga atataccaat gcttcccagc 1260
aagtatctgc acctgaagta cttaaagatt tctcttgcgg cgtacacctt tccttcaaca 1320
tttgattatt tttctctggc ttcctttttt gatgcatgtc cctctttgga ggcttttttc 1380
ttggatgcat atcagcggaa gatgaaacat gtttcgatca ttacaaatcc tttggttctg 1440
agggggactc ctgaacaacg ccatcacaag ctcaagactg ttgagatgaa tggattcact 1500
tccgctaaga gcctggttga gctagcatgc cacattgctc agtgtgcaac atcgctgcaa 1560
tccctcaaat tggaggtcca tcagagtgat tttaagtgtt atattccggc taataaacat 1620
aataagtgct ccccgctacc tgtcgaggtt ctgatggaat ctcgacgagc acacttggcc 1680
atcatgacgt acattgagcc tataattccc tccactgtga agttacaggt tgtaggacct 1740
tgcagcagat gccatgccct tgaacagtag 1770
<210> 72
<211> 589
<212> PRT
<213> Zea mays
<400> 72
Met Pro Leu Arg Asp Cys Asn Val Leu Ile Arg Thr Leu Ala Arg Arg
1 5 10 15
Gly Ser Phe Ser Arg Val Met Ala Val Tyr Tyr Asp Leu Arg Ala Arg
20 25 30
Gly Leu Ala Ala Asp Ser Tyr Thr Tyr Pro Phe Val Leu Met Ala Ile
35 40 45
Ser Thr Met Lys Leu Ser Val Glu Gly Arg Lys Val His Ala Ala Ala
50 55 60
Val Lys Thr Gly Phe Arg Trp Asp Ala Tyr Thr Thr Cys Ser Leu Met
65 70 75 80
Glu Met Tyr Thr Met Leu Gly Arg Arg Val Phe Asp Lys Met Pro Gln
85 90 95
Arg Phe Leu Val Leu Trp Asn Met Met Met Ser Tyr Lys Glu Lys Arg
100 105 110
Ser Cys Gln His Asn Asp Asp Ser Lys Gly Glu Gln Glu Lys Pro Phe
115 120 125
Leu Ser Glu Gly Val Trp Ser His Ile His Ser Phe Met His Ile Arg
130 135 140
Asp Ala Gly Arg Ala Ala Cys Leu Ser Arg Ser Phe Leu Gln Ser Trp
145 150 155 160
Arg Cys His Pro Tyr Leu Ile Phe Thr Asp Glu Thr Leu Gly Cys Lys
165 170 175
Lys Asn Thr Ser Gln Glu Asn Glu Ile Ala Ser Asn Phe Tyr Ser Arg
180 185 190
Val Asp Asn Ile Leu Asn Asn His Ser Gly Ile Gly Met Lys Ala Leu
195 200 205
Lys Ile His Val Pro Ile Gly Tyr Thr Ala Lys Asp Ser Arg Tyr Leu
210 215 220
Asn Arg Trp Leu Gln Ile Ala Ile Thr His Gly Ile Glu Glu Leu Glu
225 230 235 240
Leu Ile Phe Pro Phe Met Ala Lys Tyr Lys Phe Pro Tyr Ser Leu Leu
245 250 255
Pro Met Ser Gly Asp Ser Ile Lys Cys Leu Glu Leu Ser Cys Cys Ser
260 265 270
Phe Cys Pro Thr Ser Lys Phe Gly Trp Leu Lys Asn Leu Thr Lys Leu
275 280 285
Cys Leu Asp Gly Val Phe Ile Glu Gly Asp Glu Leu Glu Arg Leu Val
290 295 300
Phe Ser Ala Leu Ala Leu Glu Arg Leu Glu Ile Arg Tyr Cys Asp Arg
305 310 315 320
Ile Val Cys Leu Lys Val Pro Ser Met Leu Gln Arg Leu Thr Tyr Leu
325 330 335
Glu Val Tyr Asp Cys Asp Lys Leu Arg Val Leu Asp Ile Asp Ala Pro
340 345 350
Asn Ile Thr Cys Phe Asn Phe Gly Arg His His Thr Lys Thr Lys Leu
355 360 365
Ser Ile Gly Gly Gly Val Ser Lys Ile Lys Glu Leu Ser Ala Cys Phe
370 375 380
Asp Asp Ala Val Tyr Tyr Val Arg Val Glu Leu Pro Ser Met Met Ala
385 390 395 400
Asn Leu Glu Thr Leu Thr Ile Asn Ser Met Val Glu Met Asn Ile Pro
405 410 415
Met Leu Pro Ser Lys Tyr Leu His Leu Lys Tyr Leu Lys Ile Ser Leu
420 425 430
Ala Ala Tyr Thr Phe Pro Ser Thr Phe Asp Tyr Phe Ser Leu Ala Ser
435 440 445
Phe Phe Asp Ala Cys Pro Ser Leu Glu Ala Phe Phe Leu Asp Ala Tyr
450 455 460
Gln Arg Lys Met Lys His Val Ser Ile Ile Thr Asn Pro Leu Val Leu
465 470 475 480
Arg Gly Thr Pro Glu Gln Arg His His Lys Leu Lys Thr Val Glu Met
485 490 495
Asn Gly Phe Thr Ser Ala Lys Ser Leu Val Glu Leu Ala Cys His Ile
500 505 510
Ala Gln Cys Ala Thr Ser Leu Gln Ser Leu Lys Leu Glu Val His Gln
515 520 525
Ser Asp Phe Lys Cys Tyr Ile Pro Ala Asn Lys His Asn Lys Cys Ser
530 535 540
Pro Leu Pro Val Glu Val Leu Met Glu Ser Arg Arg Ala His Leu Ala
545 550 555 560
Ile Met Thr Tyr Ile Glu Pro Ile Ile Pro Ser Thr Val Lys Leu Gln
565 570 575
Val Val Gly Pro Cys Ser Arg Cys His Ala Leu Glu Gln
580 585
<210> 73
<211> 1386
<212> DNA
<213> Sorghum bicolor
<400> 73
atgccaattc aagatgctgc ccgagttgcc tgtgtgtctc acaccttttt gcgttcctgg 60
agatgccgcc ctaacctcga tttcagtagg gaaacattgg gcttaataaa tgcgtgcaga 120
aatgaggaaa cacaaaggaa catcaccagc atagttgatc acattctgca aaaccactca 180
ggcattggag tgaaggcagt caagtttcaa gatgattcat attacaatat tcaagactta 240
tgtcatcttg atctcgaaga tgattcattt tacgataatc aagacttctg tcatcttgat 300
cttgaccgtt ggcttcggaa tactattaaa ccagggattg aagaactgaa tatttctctg 360
catggaaaga atacaatgta caactttcca tgctcacttt tatctgatga aattggagag 420
tcactccgta atcttcacct cgtcggctgt tactttcatc ccacaatcgg acttggttcc 480
ttgagaaact tgagaagaat acagctgtgt aacgtatcta ttaccgagag tcagctcgag 540
tgtcttcttt ccaattcttt ctctttggag cagttggtac tcaggaattg cggtgggatt 600
atttgcctga aaataccttg cctgcagcgg cttagctacc ttgaggtgaa ctcttgcagc 660
cgactggaag ttatagagag caaagctcca aatctatcta gtgttcgcat tgcagactac 720
ctccatgtac aactctcact tttggaaacg tcgcgtatta aaaaatattt tagatcatgt 780
cctggtgctg ccttttatgc tcgtactgag cttccatcca gcatgccaaa tcttgagtct 840
cttagcttat cttcagcaac tgagacgatt aatacgccgc taatgcctag caaattcctc 900
cacctcaagt tcttgactat tgctcttcgt ggacagacct atgattgttt ttctctgtta 960
tccttttttg atgcttctcc tttcctggag actttcatat tgaatgtatt atcatcggta 1020
catgtgaagc ctgcctcagt ttttgaagat ccctcggatc tgaggaagat gccagagaac 1080
cataatgaca agctcaagtg tgttcgaatc atcaatttct catctgcaaa gagcttgatt 1140
gagttgacct gtcatattct cgagtctgca atgtcactcg agtgcctcac attggacacc 1200
actcatggtg cgcctagatg ctctgttacc aaacatggca gatgctggcg aatgacgagg 1260
gatgaaatta tggaagcgca tagagcacta ttggctgtcc aaacatacgt taagtcaaaa 1320
gttccttcaa cagtcgagtt aaatgttctt gagccctgca gccaatgcca tgctgttgca 1380
ctttag 1386
<210> 74
<211> 461
<212> PRT
<213> Sorghum bicolor
<400> 74
Met Pro Ile Gln Asp Ala Ala Arg Val Ala Cys Val Ser His Thr Phe
1 5 10 15
Leu Arg Ser Trp Arg Cys Arg Pro Asn Leu Asp Phe Ser Arg Glu Thr
20 25 30
Leu Gly Leu Ile Asn Ala Cys Arg Asn Glu Glu Thr Gln Arg Asn Ile
35 40 45
Thr Ser Ile Val Asp His Ile Leu Gln Asn His Ser Gly Ile Gly Val
50 55 60
Lys Ala Val Lys Phe Gln Asp Asp Ser Tyr Tyr Asn Ile Gln Asp Leu
65 70 75 80
Cys His Leu Asp Leu Glu Asp Asp Ser Phe Tyr Asp Asn Gln Asp Phe
85 90 95
Cys His Leu Asp Leu Asp Arg Trp Leu Arg Asn Thr Ile Lys Pro Gly
100 105 110
Ile Glu Glu Leu Asn Ile Ser Leu His Gly Lys Asn Thr Met Tyr Asn
115 120 125
Phe Pro Cys Ser Leu Leu Ser Asp Glu Ile Gly Glu Ser Leu Arg Asn
130 135 140
Leu His Leu Val Gly Cys Tyr Phe His Pro Thr Ile Gly Leu Gly Ser
145 150 155 160
Leu Arg Asn Leu Arg Arg Ile Gln Leu Cys Asn Val Ser Ile Thr Glu
165 170 175
Ser Gln Leu Glu Cys Leu Leu Ser Asn Ser Phe Ser Leu Glu Gln Leu
180 185 190
Val Leu Arg Asn Cys Gly Gly Ile Ile Cys Leu Lys Ile Pro Cys Leu
195 200 205
Gln Arg Leu Ser Tyr Leu Glu Val Asn Ser Cys Ser Arg Leu Glu Val
210 215 220
Ile Glu Ser Lys Ala Pro Asn Leu Ser Ser Val Arg Ile Ala Asp Tyr
225 230 235 240
Leu His Val Gln Leu Ser Leu Leu Glu Thr Ser Arg Ile Lys Lys Tyr
245 250 255
Phe Arg Ser Cys Pro Gly Ala Ala Phe Tyr Ala Arg Thr Glu Leu Pro
260 265 270
Ser Ser Met Pro Asn Leu Glu Ser Leu Ser Leu Ser Ser Ala Thr Glu
275 280 285
Thr Ile Asn Thr Pro Leu Met Pro Ser Lys Phe Leu His Leu Lys Phe
290 295 300
Leu Thr Ile Ala Leu Arg Gly Gln Thr Tyr Asp Cys Phe Ser Leu Leu
305 310 315 320
Ser Phe Phe Asp Ala Ser Pro Phe Leu Glu Thr Phe Ile Leu Asn Val
325 330 335
Leu Ser Ser Val His Val Lys Pro Ala Ser Val Phe Glu Asp Pro Ser
340 345 350
Asp Leu Arg Lys Met Pro Glu Asn His Asn Asp Lys Leu Lys Cys Val
355 360 365
Arg Ile Ile Asn Phe Ser Ser Ala Lys Ser Leu Ile Glu Leu Thr Cys
370 375 380
His Ile Leu Glu Ser Ala Met Ser Leu Glu Cys Leu Thr Leu Asp Thr
385 390 395 400
Thr His Gly Ala Pro Arg Cys Ser Val Thr Lys His Gly Arg Cys Trp
405 410 415
Arg Met Thr Arg Asp Glu Ile Met Glu Ala His Arg Ala Leu Leu Ala
420 425 430
Val Gln Thr Tyr Val Lys Ser Lys Val Pro Ser Thr Val Glu Leu Asn
435 440 445
Val Leu Glu Pro Cys Ser Gln Cys His Ala Val Ala Leu
450 455 460
<210> 75
<211> 1182
<212> DNA
<213> Oryza sativa
<400> 75
atgagtctac cgcttacaag aaaagatctc atgatagtca atatgggccc tcagcaccca 60
tcaatgcatg gtgttcttcg actgatcgtt actctcgatg gtgaagatgt tattgattgt 120
gaacccatat taggctattt acacagagga atggaaaaaa tcgcggaaaa ccgaactatt 180
atacaatact taccttatgt aacacgttgg gattatttag ctactatgtt tacagaagca 240
ataacggtaa atgcaccaga attcttggag aatattcaaa taccccaaag agccagctat 300
attagggtaa ttatgttaga attgagccgt atagcttctc acttgttatg gcttggacct 360
tttatggcag atctcggtgc gcagactcct tttttttata tttttagaga gagagaatta 420
atatatgatc tatttgaagc tgctacaggt atgcgaatga tgcataatta ctttcgcatc 480
ggaggagttg ctgccgatct gccttatgga tggatcgata aatgtttaga tttctgtgat 540
tattttttac gaggagttat tgaatatcaa caacttatta cacagaatcc catttttttg 600
gaacgagttg agggagttgg ttttattagc ggagaagaag ctgtaaattg gggcttatcg 660
ggccccatgt tacgagcttc tggaatacaa tgggatcttc gtaaagttga tctttacgag 720
tcttacaatc aattcgattg gaaagtccaa tggcaaaaag aaggggattc attagcacgc 780
tatttagtac gaatcggtga aatgagggaa tcaatcaaaa ttattcaaca ggctgtagaa 840
aaaattcctg ggggccctta tgagaattta gaagtccgat gctttaagaa agcaaagaat 900
tccgaatgga atgattttga atatcgattt cttggtaaaa aaccttcacc caattttgaa 960
ttgtcaaaac aagagcttta tgcaagagtg gaagccccaa aaggtgaatt aggaatttat 1020
ctggtaggag atgatagtct tttcccctgg agatggaaaa ttcgtccacc cggttttatt 1080
aatttgcaaa ttcttcctca gctagtcaaa aaaatgaaat tggctgatat catgacgata 1140
ttaggtagta tagatatcat tatgggagaa gttgatcgtt ga 1182
<210> 76
<211> 393
<212> PRT
<213> Oryza sativa
<400> 76
Met Ser Leu Pro Leu Thr Arg Lys Asp Leu Met Ile Val Asn Met Gly
1 5 10 15
Pro Gln His Pro Ser Met His Gly Val Leu Arg Leu Ile Val Thr Leu
20 25 30
Asp Gly Glu Asp Val Ile Asp Cys Glu Pro Ile Leu Gly Tyr Leu His
35 40 45
Arg Gly Met Glu Lys Ile Ala Glu Asn Arg Thr Ile Ile Gln Tyr Leu
50 55 60
Pro Tyr Val Thr Arg Trp Asp Tyr Leu Ala Thr Met Phe Thr Glu Ala
65 70 75 80
Ile Thr Val Asn Ala Pro Glu Phe Leu Glu Asn Ile Gln Ile Pro Gln
85 90 95
Arg Ala Ser Tyr Ile Arg Val Ile Met Leu Glu Leu Ser Arg Ile Ala
100 105 110
Ser His Leu Leu Trp Leu Gly Pro Phe Met Ala Asp Leu Gly Ala Gln
115 120 125
Thr Pro Phe Phe Tyr Ile Phe Arg Glu Arg Glu Leu Ile Tyr Asp Leu
130 135 140
Phe Glu Ala Ala Thr Gly Met Arg Met Met His Asn Tyr Phe Arg Ile
145 150 155 160
Gly Gly Val Ala Ala Asp Leu Pro Tyr Gly Trp Ile Asp Lys Cys Leu
165 170 175
Asp Phe Cys Asp Tyr Phe Leu Arg Gly Val Ile Glu Tyr Gln Gln Leu
180 185 190
Ile Thr Gln Asn Pro Ile Phe Leu Glu Arg Val Glu Gly Val Gly Phe
195 200 205
Ile Ser Gly Glu Glu Ala Val Asn Trp Gly Leu Ser Gly Pro Met Leu
210 215 220
Arg Ala Ser Gly Ile Gln Trp Asp Leu Arg Lys Val Asp Leu Tyr Glu
225 230 235 240
Ser Tyr Asn Gln Phe Asp Trp Lys Val Gln Trp Gln Lys Glu Gly Asp
245 250 255
Ser Leu Ala Arg Tyr Leu Val Arg Ile Gly Glu Met Arg Glu Ser Ile
260 265 270
Lys Ile Ile Gln Gln Ala Val Glu Lys Ile Pro Gly Gly Pro Tyr Glu
275 280 285
Asn Leu Glu Val Arg Cys Phe Lys Lys Ala Lys Asn Ser Glu Trp Asn
290 295 300
Asp Phe Glu Tyr Arg Phe Leu Gly Lys Lys Pro Ser Pro Asn Phe Glu
305 310 315 320
Leu Ser Lys Gln Glu Leu Tyr Ala Arg Val Glu Ala Pro Lys Gly Glu
325 330 335
Leu Gly Ile Tyr Leu Val Gly Asp Asp Ser Leu Phe Pro Trp Arg Trp
340 345 350
Lys Ile Arg Pro Pro Gly Phe Ile Asn Leu Gln Ile Leu Pro Gln Leu
355 360 365
Val Lys Lys Met Lys Leu Ala Asp Ile Met Thr Ile Leu Gly Ser Ile
370 375 380
Asp Ile Ile Met Gly Glu Val Asp Arg
385 390
<210> 77
<211> 1182
<212> DNA
<213> Zea mays
<400> 77
atgagtctat cgcttaaaag aaaagatctc atgatagtca atatgggccc tcaacaccca 60
tcaatgcatg gtgttcttcg actgattgtt actctcgatg gtgaagatgt tattgattgc 120
gaacccatat tagggtattt acacagagga atggaaaaaa tcgcggaaaa cagaagtatt 180
atacaatact tgccttatgt aacacgatgg gattatttag ctactatgtt tacagaagca 240
ataacggtaa atgcaccaga attcttagag aatattcaaa tacccaaaag agccagctat 300
attagagtaa ttatgttaga attgagccgt atagcttctc atttgttatg gcttggacct 360
tttatggcgg atctcggggc acagactccc tttttctaca tttttagaga gagagaattg 420
atatatgatc tatttgaagc tgctacaggt atgcgaatga tgcataatta ctttcgcatc 480
ggaggagtcg ctgccgatct cccttatgga tggatggata aatgtttaga tttctgtgat 540
tattttttac aaggagttgt tgaatatcaa gaacttatta cacagaatcc cattttttta 600
gaacgagttg aaggagtcgg ttttattagc ggagaagaag ctgtaaattg gggcttatcg 660
ggaccaatgt tacgagcttc tggaatacaa tgggatcttc gtaaaattga tccttatgag 720
tcttacaatc aattcgattg gaaagtccaa tggcaaaaag aaggagattc gttagctcgc 780
tatttagtac gagtcggtga aatgagggaa tccataaaaa ttattcaaca ggctgtagag 840
aaaattcctg gaggacctta tgagaattta gaagcccgac gctttaagaa agcaaagaat 900
cccgaatgga atgattttga atatcgattt cttggtaaaa aaccttcgcc caattttgaa 960
ttatcaaagc aagagcttta tgtaagagta gaagctccaa aaggcgaatt aggaatttat 1020
ctggtaggag atgatagtct tttcccctgg agatggaaaa ttcgtccacc gggttttatt 1080
aatttgcaaa ttcttcctca gctagttaaa aaaatgaaat tggctgatat catgacaata 1140
ttaggtagta tagatatcat tatgggggaa gttgatcgtt ga 1182
<210> 78
<211> 393
<212> PRT
<213> Zea mays
<400> 78
Met Ser Leu Ser Leu Lys Arg Lys Asp Leu Met Ile Val Asn Met Gly
1 5 10 15
Pro Gln His Pro Ser Met His Gly Val Leu Arg Leu Ile Val Thr Leu
20 25 30
Asp Gly Glu Asp Val Ile Asp Cys Glu Pro Ile Leu Gly Tyr Leu His
35 40 45
Arg Gly Met Glu Lys Ile Ala Glu Asn Arg Ser Ile Ile Gln Tyr Leu
50 55 60
Pro Tyr Val Thr Arg Trp Asp Tyr Leu Ala Thr Met Phe Thr Glu Ala
65 70 75 80
Ile Thr Val Asn Ala Pro Glu Phe Leu Glu Asn Ile Gln Ile Pro Lys
85 90 95
Arg Ala Ser Tyr Ile Arg Val Ile Met Leu Glu Leu Ser Arg Ile Ala
100 105 110
Ser His Leu Leu Trp Leu Gly Pro Phe Met Ala Asp Leu Gly Ala Gln
115 120 125
Thr Pro Phe Phe Tyr Ile Phe Arg Glu Arg Glu Leu Ile Tyr Asp Leu
130 135 140
Phe Glu Ala Ala Thr Gly Met Arg Met Met His Asn Tyr Phe Arg Ile
145 150 155 160
Gly Gly Val Ala Ala Asp Leu Pro Tyr Gly Trp Met Asp Lys Cys Leu
165 170 175
Asp Phe Cys Asp Tyr Phe Leu Gln Gly Val Val Glu Tyr Gln Glu Leu
180 185 190
Ile Thr Gln Asn Pro Ile Phe Leu Glu Arg Val Glu Gly Val Gly Phe
195 200 205
Ile Ser Gly Glu Glu Ala Val Asn Trp Gly Leu Ser Gly Pro Met Leu
210 215 220
Arg Ala Ser Gly Ile Gln Trp Asp Leu Arg Lys Ile Asp Pro Tyr Glu
225 230 235 240
Ser Tyr Asn Gln Phe Asp Trp Lys Val Gln Trp Gln Lys Glu Gly Asp
245 250 255
Ser Leu Ala Arg Tyr Leu Val Arg Val Gly Glu Met Arg Glu Ser Ile
260 265 270
Lys Ile Ile Gln Gln Ala Val Glu Lys Ile Pro Gly Gly Pro Tyr Glu
275 280 285
Asn Leu Glu Ala Arg Arg Phe Lys Lys Ala Lys Asn Pro Glu Trp Asn
290 295 300
Asp Phe Glu Tyr Arg Phe Leu Gly Lys Lys Pro Ser Pro Asn Phe Glu
305 310 315 320
Leu Ser Lys Gln Glu Leu Tyr Val Arg Val Glu Ala Pro Lys Gly Glu
325 330 335
Leu Gly Ile Tyr Leu Val Gly Asp Asp Ser Leu Phe Pro Trp Arg Trp
340 345 350
Lys Ile Arg Pro Pro Gly Phe Ile Asn Leu Gln Ile Leu Pro Gln Leu
355 360 365
Val Lys Lys Met Lys Leu Ala Asp Ile Met Thr Ile Leu Gly Ser Ile
370 375 380
Asp Ile Ile Met Gly Glu Val Asp Arg
385 390
<210> 79
<211> 1182
<212> DNA
<213> Arabidopsis thaliana
<400> 79
atgaagagac cagttacagg aaaagatctt atgatagtca atatgggacc tcaccaccca 60
tccatgcatg gtgttcttcg cttaattgtt actctagatg gtgaggatgt tgttgactgt 120
gaacccatat taggttattt acacagagga atggaaaaaa ttgcagaaaa ccgagcaatt 180
atacaatatt taccgtatgt aacgcgatgg gattatttag ctactatgtt tacagaagca 240
ataacagtaa atggaccaga acaattggga aatattcaag ttcctaaaag ggccagctat 300
atcagagtaa ttatgctgga attgagtcgt atagcttctc atctgttatg gctcggccct 360
tttatggcag atattggtgc acagactccc tttttctata ttttcagaga acgagaattt 420
gtatatgatc tattcgaagc tgccaccggt atgagaatga tgcataattt ttttcgtatt 480
ggaggaatag cggctgattt accttatggt tggatagata aatgcttgga tttttgtgat 540
tattttttaa cagaggttgt tgaatatcaa aaactcatta cacgaaatcc tattttttta 600
gaacgggttg aaggagttgg gattattggt ggggaagaag caataaattg gggtttatcc 660
ggaccaatgc tacgcgcatc cggaatacca tgggatcttc gtaaaattga tcgttatgag 720
tcttacgatg aatttgaatg ggaaattcaa tggcaaaaac aaggagattc attagctcgt 780
tatttagtac gacttagcga aatgacagaa tccataaaaa ttattcaaca ggctctggaa 840
ggacttccag ggggtcccta tgagaattta gaaagcaggg gctttgatag aaaaaggaat 900
ccagaatgga atgattttga atatcgattc attagtaaaa aaccttctcc tacttttgaa 960
ttatcaaaac aagaacttta tgtaagagtt gaagctccaa aaggggagtt gggaattttt 1020
ctcataggag atcaaagcgg ttttccttgg agatggaaaa tacgaccacc gggttttatt 1080
aatttgcaaa ttcttcctga actagttaaa agaatgaaat tggctgatat tatgacgata 1140
ctcggtagca tagatataat tatgggagaa gttgatcgtt aa 1182
<210> 80
<211> 393
<212> PRT
<213> Arabidopsis thaliana
<400> 80
Met Lys Arg Pro Val Thr Gly Lys Asp Leu Met Ile Val Asn Met Gly
1 5 10 15
Pro His His Pro Ser Met His Gly Val Leu Arg Leu Ile Val Thr Leu
20 25 30
Asp Gly Glu Asp Val Val Asp Cys Glu Pro Ile Leu Gly Tyr Leu His
35 40 45
Arg Gly Met Glu Lys Ile Ala Glu Asn Arg Ala Ile Ile Gln Tyr Leu
50 55 60
Pro Tyr Val Thr Arg Trp Asp Tyr Leu Ala Thr Met Phe Thr Glu Ala
65 70 75 80
Ile Thr Val Asn Gly Pro Glu Gln Leu Gly Asn Ile Gln Val Pro Lys
85 90 95
Arg Ala Ser Tyr Ile Arg Val Ile Met Leu Glu Leu Ser Arg Ile Ala
100 105 110
Ser His Leu Leu Trp Leu Gly Pro Phe Met Ala Asp Ile Gly Ala Gln
115 120 125
Thr Pro Phe Phe Tyr Ile Phe Arg Glu Arg Glu Phe Val Tyr Asp Leu
130 135 140
Phe Glu Ala Ala Thr Gly Met Arg Met Met His Asn Phe Phe Arg Ile
145 150 155 160
Gly Gly Ile Ala Ala Asp Leu Pro Tyr Gly Trp Ile Asp Lys Cys Leu
165 170 175
Asp Phe Cys Asp Tyr Phe Leu Thr Glu Val Val Glu Tyr Gln Lys Leu
180 185 190
Ile Thr Arg Asn Pro Ile Phe Leu Glu Arg Val Glu Gly Val Gly Ile
195 200 205
Ile Gly Gly Glu Glu Ala Ile Asn Trp Gly Leu Ser Gly Pro Met Leu
210 215 220
Arg Ala Ser Gly Ile Pro Trp Asp Leu Arg Lys Ile Asp Arg Tyr Glu
225 230 235 240
Ser Tyr Asp Glu Phe Glu Trp Glu Ile Gln Trp Gln Lys Gln Gly Asp
245 250 255
Ser Leu Ala Arg Tyr Leu Val Arg Leu Ser Glu Met Thr Glu Ser Ile
260 265 270
Lys Ile Ile Gln Gln Ala Leu Glu Gly Leu Pro Gly Gly Pro Tyr Glu
275 280 285
Asn Leu Glu Ser Arg Gly Phe Asp Arg Lys Arg Asn Pro Glu Trp Asn
290 295 300
Asp Phe Glu Tyr Arg Phe Ile Ser Lys Lys Pro Ser Pro Thr Phe Glu
305 310 315 320
Leu Ser Lys Gln Glu Leu Tyr Val Arg Val Glu Ala Pro Lys Gly Glu
325 330 335
Leu Gly Ile Phe Leu Ile Gly Asp Gln Ser Gly Phe Pro Trp Arg Trp
340 345 350
Lys Ile Arg Pro Pro Gly Phe Ile Asn Leu Gln Ile Leu Pro Glu Leu
355 360 365
Val Lys Arg Met Lys Leu Ala Asp Ile Met Thr Ile Leu Gly Ser Ile
370 375 380
Asp Ile Ile Met Gly Glu Val Asp Arg
385 390
<210> 81
<211> 1308
<212> DNA
<213> Oryza sativa
<400> 81
atggcgcggt tggaagatat ggtggaggca aggttgagtg ttctaagagg agtccaatcg 60
agaacgaatc agacgttcac catcttccgc gtccccgcct ttcttcgcga gagcagccgg 120
acctcctacg agccgcgctt ggtgtccatc ggcccctact accacggcgc cgccgcgctc 180
cgcgccatgg aggaccacaa gtggcgctac ctccacgacc tgctgtctcc gcaaggaccg 240
ccggaaccca accatcgcgt tccacttcca ccccttgtct ccggactctc cgcatccgcc 300
ttggttgaga agatgcggtc tctcgaggcc gaggcgcgcg cctgctacag cgagcaaccc 360
gtcgacctca gctccgacga cttcgtccag atgctcctcc tggacggatg cttcatcctc 420
gagttcttcc gtaagtggcg cagaaatcag cccgatgtgc tatgcgacgt tggttggggc 480
ctcacgttcg tcatctccga tttgctcctt atggagaatc agctcccgtt cttcgtcctc 540
aagaagttat atgttaccgc ctttggcgaa caggatggcc aggccggaaa taacctcctc 600
cagcttctcc ttcagtacat cgccggccgt caagttccaa tcagatggcc gaacgggcag 660
gtcaatcaca tcctacacct gtactacgag agcttcgtgc cccagagcca gcggacgccg 720
cagcaagaac agagtaccac ggcgccgcga gtgctaccgt gcgccgtcga gatgagcgaa 780
gccggggtga ctttcgccgt gcggcggaac tcagacaacg gctacgacgt ggtgttcgac 840
tcccttaggg gcgtcatgga gatacccacc atattgatcg acgacgcgaa gacgccgctg 900
ctcgcgaacc tgatcgcgtt cgagcagagc ctgggcaacg acgaggccat cctcctcagc 960
agctacgtgg cgctgatggg ccagctcatc gtcacggcgc gcgacgtcgc gctgctccgc 1020
cggcgcggcg tgctggagaa catgctggcc aacgacgacg atgccgcccg gttcttcaac 1080
cacctcggcg actgtggcgc cgtcaaccac gacagccacg ccttcgtcgg gctctacaag 1140
gacgtggacc gctactgcgg gacatggtgg cggaggaaga cggcggcgct ccggcgcgac 1200
tacttcgcca gcccgtggtc ggccatctcc ttcgtcgccg ccgcagtcgc cgtcgtgctc 1260
gcagttatgc agacctactt caccatgttt ccattgaaaa aagggtga 1308
<210> 82
<211> 435
<212> PRT
<213> Oryza sativa
<400> 82
Met Ala Arg Leu Glu Asp Met Val Glu Ala Arg Leu Ser Val Leu Arg
1 5 10 15
Gly Val Gln Ser Arg Thr Asn Gln Thr Phe Thr Ile Phe Arg Val Pro
20 25 30
Ala Phe Leu Arg Glu Ser Ser Arg Thr Ser Tyr Glu Pro Arg Leu Val
35 40 45
Ser Ile Gly Pro Tyr Tyr His Gly Ala Ala Ala Leu Arg Ala Met Glu
50 55 60
Asp His Lys Trp Arg Tyr Leu His Asp Leu Leu Ser Pro Gln Gly Pro
65 70 75 80
Pro Glu Pro Asn His Arg Val Pro Leu Pro Pro Leu Val Ser Gly Leu
85 90 95
Ser Ala Ser Ala Leu Val Glu Lys Met Arg Ser Leu Glu Ala Glu Ala
100 105 110
Arg Ala Cys Tyr Ser Glu Gln Pro Val Asp Leu Ser Ser Asp Asp Phe
115 120 125
Val Gln Met Leu Leu Leu Asp Gly Cys Phe Ile Leu Glu Phe Phe Arg
130 135 140
Lys Trp Arg Arg Asn Gln Pro Asp Val Leu Cys Asp Val Gly Trp Gly
145 150 155 160
Leu Thr Phe Val Ile Ser Asp Leu Leu Leu Met Glu Asn Gln Leu Pro
165 170 175
Phe Phe Val Leu Lys Lys Leu Tyr Val Thr Ala Phe Gly Glu Gln Asp
180 185 190
Gly Gln Ala Gly Asn Asn Leu Leu Gln Leu Leu Leu Gln Tyr Ile Ala
195 200 205
Gly Arg Gln Val Pro Ile Arg Trp Pro Asn Gly Gln Val Asn His Ile
210 215 220
Leu His Leu Tyr Tyr Glu Ser Phe Val Pro Gln Ser Gln Arg Thr Pro
225 230 235 240
Gln Gln Glu Gln Ser Thr Thr Ala Pro Arg Val Leu Pro Cys Ala Val
245 250 255
Glu Met Ser Glu Ala Gly Val Thr Phe Ala Val Arg Arg Asn Ser Asp
260 265 270
Asn Gly Tyr Asp Val Val Phe Asp Ser Leu Arg Gly Val Met Glu Ile
275 280 285
Pro Thr Ile Leu Ile Asp Asp Ala Lys Thr Pro Leu Leu Ala Asn Leu
290 295 300
Ile Ala Phe Glu Gln Ser Leu Gly Asn Asp Glu Ala Ile Leu Leu Ser
305 310 315 320
Ser Tyr Val Ala Leu Met Gly Gln Leu Ile Val Thr Ala Arg Asp Val
325 330 335
Ala Leu Leu Arg Arg Arg Gly Val Leu Glu Asn Met Leu Ala Asn Asp
340 345 350
Asp Asp Ala Ala Arg Phe Phe Asn His Leu Gly Asp Cys Gly Ala Val
355 360 365
Asn His Asp Ser His Ala Phe Val Gly Leu Tyr Lys Asp Val Asp Arg
370 375 380
Tyr Cys Gly Thr Trp Trp Arg Arg Lys Thr Ala Ala Leu Arg Arg Asp
385 390 395 400
Tyr Phe Ala Ser Pro Trp Ser Ala Ile Ser Phe Val Ala Ala Ala Val
405 410 415
Ala Val Val Leu Ala Val Met Gln Thr Tyr Phe Thr Met Phe Pro Leu
420 425 430
Lys Lys Gly
435
<210> 83
<211> 1287
<212> DNA
<213> Zea mays
<400> 83
atggaacgcg agagcgatgc cggcttcttc caccacgacg acgcgcaggt ggaggccatg 60
cagcggcgcg tggacgcggc ggcgcccttg gccgacgacc cctacaccat cttccgcctc 120
ccctcggccg tgcgcgagcg ccggcgcgac ctgtacgagc ccaaggtcgt gtccgtgggc 180
ccgtactacc acggccgcgc cggcctgggc gtcgcgcagc agcacaagtg gcgcctcctc 240
cgcgacttcc tctcgcgggg ggagaaggcg gccgccgccg gtggcctcgg cgcgtacgtg 300
cgcgccgcgc gcgaggtcga ggcggacgcg cgccggtgct acgccgaggg gttcggcctg 360
ggcgccgacg agttcgcgga gctgctggtg ctcgacggct gcttcctcct cgagttcttc 420
ctcaagaagg gcgaggggca gctcgccgcg cccgggggcg ccaagtgggc gtggcaccac 480
atgtaccacg acgtcctcct gctggagaac cagataccct tcttcgtcgt cgagaagcta 540
cacggcgtcg ccttcgccgg cgccgaccgc gaccgcgacg cgctcctgga catcttctgc 600
aaggccttcg ccggcgacct cccgtcgagc cgcgtcatcc ggccgcgcag cgacaagacc 660
atccaccacc tgctgcacct gcactacgag tgcaacatcc gcagcccagc cgccgccgac 720
agcgacaagg cccgcagcag cagcttcggc gacgccaacg gcgcgtcgct ggccatctgg 780
aagcagccgg cgatcccgtc gccgcgctcc ggcgaaggcg ccggcgccgg cgccggcagc 840
aaaggccgtc tgacgtcgat gatcccgccg gcgggcaaga tggaggaggc cggcgtggcg 900
ttcaagcgca aggcggcccc gcgggacctg ttcgacgtca gcttccggta cggcgtgttg 960
cacgtgccgg cgttcgtgct cgacgagggc gcaaaggtgc tgctcgcgaa tctggtggcg 1020
ttcgagcagg gaggcggccg cgcggcgcgg cagctggacg ggggcaacct ggtgacgggc 1080
ttcgtggcgc ttgtcgggtc gctcgtgaac tcgcggaggg atctggaggt gctccgccgg 1140
tgcgggatca tgcactgcat gctacctgat gacgacgccg tagcctactt caaccacgtt 1200
gtacagtata ccaccatgga ctacgaccgg aaccttctcg ccagcctatt ccgagacgtt 1260
agggagcact gccattggaa cagatga 1287
<210> 84
<211> 428
<212> PRT
<213> Zea mays
<400> 84
Met Glu Arg Glu Ser Asp Ala Gly Phe Phe His His Asp Asp Ala Gln
1 5 10 15
Val Glu Ala Met Gln Arg Arg Val Asp Ala Ala Ala Pro Leu Ala Asp
20 25 30
Asp Pro Tyr Thr Ile Phe Arg Leu Pro Ser Ala Val Arg Glu Arg Arg
35 40 45
Arg Asp Leu Tyr Glu Pro Lys Val Val Ser Val Gly Pro Tyr Tyr His
50 55 60
Gly Arg Ala Gly Leu Gly Val Ala Gln Gln His Lys Trp Arg Leu Leu
65 70 75 80
Arg Asp Phe Leu Ser Arg Gly Glu Lys Ala Ala Ala Ala Gly Gly Leu
85 90 95
Gly Ala Tyr Val Arg Ala Ala Arg Glu Val Glu Ala Asp Ala Arg Arg
100 105 110
Cys Tyr Ala Glu Gly Phe Gly Leu Gly Ala Asp Glu Phe Ala Glu Leu
115 120 125
Leu Val Leu Asp Gly Cys Phe Leu Leu Glu Phe Phe Leu Lys Lys Gly
130 135 140
Glu Gly Gln Leu Ala Ala Pro Gly Gly Ala Lys Trp Ala Trp His His
145 150 155 160
Met Tyr His Asp Val Leu Leu Leu Glu Asn Gln Ile Pro Phe Phe Val
165 170 175
Val Glu Lys Leu His Gly Val Ala Phe Ala Gly Ala Asp Arg Asp Arg
180 185 190
Asp Ala Leu Leu Asp Ile Phe Cys Lys Ala Phe Ala Gly Asp Leu Pro
195 200 205
Ser Ser Arg Val Ile Arg Pro Arg Ser Asp Lys Thr Ile His His Leu
210 215 220
Leu His Leu His Tyr Glu Cys Asn Ile Arg Ser Pro Ala Ala Ala Asp
225 230 235 240
Ser Asp Lys Ala Arg Ser Ser Ser Phe Gly Asp Ala Asn Gly Ala Ser
245 250 255
Leu Ala Ile Trp Lys Gln Pro Ala Ile Pro Ser Pro Arg Ser Gly Glu
260 265 270
Gly Ala Gly Ala Gly Ala Gly Ser Lys Gly Arg Leu Thr Ser Met Ile
275 280 285
Pro Pro Ala Gly Lys Met Glu Glu Ala Gly Val Ala Phe Lys Arg Lys
290 295 300
Ala Ala Pro Arg Asp Leu Phe Asp Val Ser Phe Arg Tyr Gly Val Leu
305 310 315 320
His Val Pro Ala Phe Val Leu Asp Glu Gly Ala Lys Val Leu Leu Ala
325 330 335
Asn Leu Val Ala Phe Glu Gln Gly Gly Gly Arg Ala Ala Arg Gln Leu
340 345 350
Asp Gly Gly Asn Leu Val Thr Gly Phe Val Ala Leu Val Gly Ser Leu
355 360 365
Val Asn Ser Arg Arg Asp Leu Glu Val Leu Arg Arg Cys Gly Ile Met
370 375 380
His Cys Met Leu Pro Asp Asp Asp Ala Val Ala Tyr Phe Asn His Val
385 390 395 400
Val Gln Tyr Thr Thr Met Asp Tyr Asp Arg Asn Leu Leu Ala Ser Leu
405 410 415
Phe Arg Asp Val Arg Glu His Cys His Trp Asn Arg
420 425
<210> 85
<211> 1332
<212> DNA
<213> Sorghum bicolor
<400> 85
atggagcgcg agagaaacgg cggcgcggcg gccgcgggcg aggaggacaa cggcttcttc 60
taccacgacg acgcgcaggt ggaggccatg cagcggcgcg tggacgcggc ggcgcccttg 120
gccgacgacg acccctacac catcttccgc ctcccctcgg ctgtgcgcga gcggcaccgc 180
gacctgtacg agcccaaggt cgtgtccgtg ggcccgtact accacggccg cgccggcctg 240
ggcgccgcgc agcagcacaa gtggcgcctc ctccgcgact tcctctcgcg gtcgcggggg 300
aagaataagc aggccgccgc cggtggcggt ggcctcggcg cgtacgtgcg cgccgcgcgc 360
gaggtcgagg cggacgcgcg ccggtgctac gccgaggggt tcggcctggg cgccgacgag 420
ttcgcggagc tgctggtgct cgacggctgc ttccttctcg agttcttcct caagaagggc 480
gaggggcagc tcgccgcgcc cgggggcgcc aagtgggcgt ggcaccacat gtaccacgac 540
gtcctgctgc tggagaacca gatacccttc ttcgtcatcg agaagctgca cggcgtggcc 600
ttcgcggccg gcggcgacga cggcggcgcc gatgagcgcg acgcgctcct ggacgttttc 660
tgcaaggcct tcgccggcga cctgccgtcg agccgcgtca tccggccacg cagcgacaag 720
accatccacc acctgctgca cctgcactac gagtgcaacg tcctcaaccc agccgccgat 780
agcgacaagg cccgccgcaa cagcatcggc ggcgacgcca acagcgcgtc gctggccatc 840
tggaagcagc cggcaatccc gtcgccgcgc accggccagg gcaggaaagg ccgtctgacg 900
tcgatgatcc cgccggcggg caagatggag gaggccggcg tgacgttcaa gcgcaaggcg 960
accccgcggg acgtgttcga cgtcagcttc cggtacggcg tgctgcacgt gccggcgttc 1020
gtgatggacg agggcgccaa ggtgctgctc gcgaacctgg tggcgttcga gcagggaggt 1080
ggccgcgcgg cgcggcagct ggacgcgggc aacctggtga cgggcttcgt ggcgcttgtc 1140
ggatcgctcg tgaactcgcg gagggacttg gaggtgctcc gccggtgcgg gatcatgcat 1200
tgcatgctgg ccgacgacga cgccgtggcc tacttcaacc acgtggtaca gtatacgacc 1260
atggactacg accggcacct gctggcctgc ctattcaggg acgttcgaga gcactgccat 1320
tggaacagat ga 1332
<210> 86
<211> 443
<212> PRT
<213> Sorghum bicolor
<400> 86
Met Glu Arg Glu Arg Asn Gly Gly Ala Ala Ala Ala Gly Glu Glu Asp
1 5 10 15
Asn Gly Phe Phe Tyr His Asp Asp Ala Gln Val Glu Ala Met Gln Arg
20 25 30
Arg Val Asp Ala Ala Ala Pro Leu Ala Asp Asp Asp Pro Tyr Thr Ile
35 40 45
Phe Arg Leu Pro Ser Ala Val Arg Glu Arg His Arg Asp Leu Tyr Glu
50 55 60
Pro Lys Val Val Ser Val Gly Pro Tyr Tyr His Gly Arg Ala Gly Leu
65 70 75 80
Gly Ala Ala Gln Gln His Lys Trp Arg Leu Leu Arg Asp Phe Leu Ser
85 90 95
Arg Ser Arg Gly Lys Asn Lys Gln Ala Ala Ala Gly Gly Gly Gly Leu
100 105 110
Gly Ala Tyr Val Arg Ala Ala Arg Glu Val Glu Ala Asp Ala Arg Arg
115 120 125
Cys Tyr Ala Glu Gly Phe Gly Leu Gly Ala Asp Glu Phe Ala Glu Leu
130 135 140
Leu Val Leu Asp Gly Cys Phe Leu Leu Glu Phe Phe Leu Lys Lys Gly
145 150 155 160
Glu Gly Gln Leu Ala Ala Pro Gly Gly Ala Lys Trp Ala Trp His His
165 170 175
Met Tyr His Asp Val Leu Leu Leu Glu Asn Gln Ile Pro Phe Phe Val
180 185 190
Ile Glu Lys Leu His Gly Val Ala Phe Ala Ala Gly Gly Asp Asp Gly
195 200 205
Gly Ala Asp Glu Arg Asp Ala Leu Leu Asp Val Phe Cys Lys Ala Phe
210 215 220
Ala Gly Asp Leu Pro Ser Ser Arg Val Ile Arg Pro Arg Ser Asp Lys
225 230 235 240
Thr Ile His His Leu Leu His Leu His Tyr Glu Cys Asn Val Leu Asn
245 250 255
Pro Ala Ala Asp Ser Asp Lys Ala Arg Arg Asn Ser Ile Gly Gly Asp
260 265 270
Ala Asn Ser Ala Ser Leu Ala Ile Trp Lys Gln Pro Ala Ile Pro Ser
275 280 285
Pro Arg Thr Gly Gln Gly Arg Lys Gly Arg Leu Thr Ser Met Ile Pro
290 295 300
Pro Ala Gly Lys Met Glu Glu Ala Gly Val Thr Phe Lys Arg Lys Ala
305 310 315 320
Thr Pro Arg Asp Val Phe Asp Val Ser Phe Arg Tyr Gly Val Leu His
325 330 335
Val Pro Ala Phe Val Met Asp Glu Gly Ala Lys Val Leu Leu Ala Asn
340 345 350
Leu Val Ala Phe Glu Gln Gly Gly Gly Arg Ala Ala Arg Gln Leu Asp
355 360 365
Ala Gly Asn Leu Val Thr Gly Phe Val Ala Leu Val Gly Ser Leu Val
370 375 380
Asn Ser Arg Arg Asp Leu Glu Val Leu Arg Arg Cys Gly Ile Met His
385 390 395 400
Cys Met Leu Ala Asp Asp Asp Ala Val Ala Tyr Phe Asn His Val Val
405 410 415
Gln Tyr Thr Thr Met Asp Tyr Asp Arg His Leu Leu Ala Cys Leu Phe
420 425 430
Arg Asp Val Arg Glu His Cys His Trp Asn Arg
435 440
<210> 87
<211> 1323
<212> DNA
<213> Arabidopsis thaliana
<400> 87
atggatccac agattgttgt agacataaag agtttaatct cagaagataa tggtttgaag 60
cttcttagag aatcagctgg ttcagagtta tgttgcatcg tgagaatccc acagagcctc 120
gctcgaatca acctcaaagc ttatgaaccg aagattgtct ccattggtcc ttaccatcac 180
ggcaaagagc atctcaaaat gactcagcag cataaacgcc gattcttgaa gtttttcgtg 240
gctaaaatgg aagaaaaagg atttgttcct caagaattgg tcaaagctgt atcgagtttg 300
gaaggagtta tacgaggttc ttactccgag gatctaggtt tagattctga aaacttggtt 360
cagatgatgg ttcttgatgg ttgctttatc ctcacgttgt tctttgttgt ttccgggaaa 420
gttgaataca ctaatcttga tgatccgatt tttcgaatgc cgtggatttt gccgtcgatt 480
cgagcagatc ttctcctttt ggaaaaccag gttccttatg ttcttcttca aactctattt 540
gaaacatcga agttagttac ttgtagtggt ttaaacgaaa tcgcctttga attctttaac 600
tactcattac aaaaaccaga gaccttttgg gaaaaacatt atggtcttga agcaaaacat 660
cttcttgatt tgatacgtaa gacttttgtt cctgttccgt ctcaaagaag aatcaaagat 720
catagcagta aatcgtcatt caatgatcat gaatatctcg gatttgttct ctctgccaag 780
aagcttcatc ttcggggaat caaattcaaa ccgaggaaaa acacagactc aatcttagac 840
attagttata gtaatggtgt gcttcacatt cctccggtag tcatggatga ttttaccgcc 900
tcgatctttt taaactgtgt cgcatttgag caattatatg ctgattcatc gaaccacata 960
acgagctacg tagctttcat ggcttgtctt ataaacgaag agagtgatgc atcgttcctt 1020
agcgaaagga ggatcctaga gaactatttt ggaacagagg atgaagtgtc ccggttctat 1080
aaacgcattg gtaaagacat tgccctcgac ttagagaaga gttacctagc aaaggtgttt 1140
gaaggagtta acgagtatac ttctcaagga ttccatgttc attgtgcaga gtttattcat 1200
acgcatttcg atagtccatg gacatttgca tcatcatttg cagcgttgtt gcttcttctg 1260
tttgcggctt tgcaagtctt ctttgcagct tatagttatt tccgtcctcc aaaagaaaag 1320
tga 1323
<210> 88
<211> 440
<212> PRT
<213> Arabidopsis thaliana
<400> 88
Met Asp Pro Gln Ile Val Val Asp Ile Lys Ser Leu Ile Ser Glu Asp
1 5 10 15
Asn Gly Leu Lys Leu Leu Arg Glu Ser Ala Gly Ser Glu Leu Cys Cys
20 25 30
Ile Val Arg Ile Pro Gln Ser Leu Ala Arg Ile Asn Leu Lys Ala Tyr
35 40 45
Glu Pro Lys Ile Val Ser Ile Gly Pro Tyr His His Gly Lys Glu His
50 55 60
Leu Lys Met Thr Gln Gln His Lys Arg Arg Phe Leu Lys Phe Phe Val
65 70 75 80
Ala Lys Met Glu Glu Lys Gly Phe Val Pro Gln Glu Leu Val Lys Ala
85 90 95
Val Ser Ser Leu Glu Gly Val Ile Arg Gly Ser Tyr Ser Glu Asp Leu
100 105 110
Gly Leu Asp Ser Glu Asn Leu Val Gln Met Met Val Leu Asp Gly Cys
115 120 125
Phe Ile Leu Thr Leu Phe Phe Val Val Ser Gly Lys Val Glu Tyr Thr
130 135 140
Asn Leu Asp Asp Pro Ile Phe Arg Met Pro Trp Ile Leu Pro Ser Ile
145 150 155 160
Arg Ala Asp Leu Leu Leu Leu Glu Asn Gln Val Pro Tyr Val Leu Leu
165 170 175
Gln Thr Leu Phe Glu Thr Ser Lys Leu Val Thr Cys Ser Gly Leu Asn
180 185 190
Glu Ile Ala Phe Glu Phe Phe Asn Tyr Ser Leu Gln Lys Pro Glu Thr
195 200 205
Phe Trp Glu Lys His Tyr Gly Leu Glu Ala Lys His Leu Leu Asp Leu
210 215 220
Ile Arg Lys Thr Phe Val Pro Val Pro Ser Gln Arg Arg Ile Lys Asp
225 230 235 240
His Ser Ser Lys Ser Ser Phe Asn Asp His Glu Tyr Leu Gly Phe Val
245 250 255
Leu Ser Ala Lys Lys Leu His Leu Arg Gly Ile Lys Phe Lys Pro Arg
260 265 270
Lys Asn Thr Asp Ser Ile Leu Asp Ile Ser Tyr Ser Asn Gly Val Leu
275 280 285
His Ile Pro Pro Val Val Met Asp Asp Phe Thr Ala Ser Ile Phe Leu
290 295 300
Asn Cys Val Ala Phe Glu Gln Leu Tyr Ala Asp Ser Ser Asn His Ile
305 310 315 320
Thr Ser Tyr Val Ala Phe Met Ala Cys Leu Ile Asn Glu Glu Ser Asp
325 330 335
Ala Ser Phe Leu Ser Glu Arg Arg Ile Leu Glu Asn Tyr Phe Gly Thr
340 345 350
Glu Asp Glu Val Ser Arg Phe Tyr Lys Arg Ile Gly Lys Asp Ile Ala
355 360 365
Leu Asp Leu Glu Lys Ser Tyr Leu Ala Lys Val Phe Glu Gly Val Asn
370 375 380
Glu Tyr Thr Ser Gln Gly Phe His Val His Cys Ala Glu Phe Ile His
385 390 395 400
Thr His Phe Asp Ser Pro Trp Thr Phe Ala Ser Ser Phe Ala Ala Leu
405 410 415
Leu Leu Leu Leu Phe Ala Ala Leu Gln Val Phe Phe Ala Ala Tyr Ser
420 425 430
Tyr Phe Arg Pro Pro Lys Glu Lys
435 440
<210> 89
<211> 1539
<212> DNA
<213> Glycine max
<400> 89
atggaacctg ctactaataa caatccttct ccttctgctc atgcttcatt acatgaaacc 60
aaatatattg agagggtgaa acatgccctg atcgactttg gagtcccaga agagctaagg 120
ttgtctgatc gtagcatcta caaggttcct tgctatctcc gaaaggtgaa ggaagatgcc 180
tacactcctc agtgcatctc aataggtcca attcacttca aaaaagaaga actcaagcca 240
atgcaagagc ataagctaag atactaccaa ttcttcggga gacgtgttgg tgtctcagac 300
gaacaaatgg aggcatacaa acactacctt gagaccgaag aaaagcagat tcgacaatgc 360
tatgcagaga aattccttga cataaccaaa gatacattcg tggacatgat gctgctggac 420
gccgtattca tcatggaact tatgttgaga aactgcgagt tcaaatcaca caaagccaag 480
catgagcaaa accacaagcg aaccgaatcc tttagaatta aaaacaacaa tgatctgatc 540
atgacacact catggcttag caggaacatc gctggggact tgatccttat agagaaccaa 600
ataccctttt ttgtgctgca gaaactctat gatgatgttg tccctcgtga aagcaagaag 660
gatgaacaca ctgcggggtt tgttaagctt gctactgagt actttgcttt ctatgacacc 720
caaatgtctt cttctggtga aaccaagaaa cactgttcct gttacatact ccactgtctg 780
aaggaacctt gtaagtcaaa aggaaaagat aggagtgaga tttcgaaacg tcctctagga 840
agcaattctg aagagaatcc agaaggacca aaacatttca ctgatctgat aaggtggcaa 900
ttttaccttc ccaccgagtg tgaagccggt catgctcatc aggttctaag aactgcaaca 960
aagttacaag gctcagggat aagctttgag aaaggtgatg tgaacaagcg gttactggaa 1020
atagccttca agaagacccc aattctgagt tcatttcttt gctttggttg ctttccattg 1080
tcgaagctct tcaaagcgcg tttgaggatt ccccaattga aggtagacca cacaactgaa 1140
cgtgtgttca agaacctcgt tgcttttgag cagtttcact atccagacaa gccttacttt 1200
tgcaactatg tttctttcat tgactctctg atacacactc agcttgatgt ggagttgctg 1260
gttgagaagg aagtgattgt gcatgaactt gggagtgata aggaagtggc aactcttgtt 1320
aatggtttat gcaaacatgt tgtcacaaac tcaacttgtt accatcacat tataaataag 1380
ctcaacgacc attacatgaa cgattggaac cacactattg cggctttaag gttggtgtac 1440
ttcagagatc tttggagggc aagtggcact gtggtgggta ttgctgtgct ggtttttgct 1500
gtcttccaat tcctccgtgt ttgtcgttat ctcttctag 1539
<210> 90
<211> 512
<212> PRT
<213> Glycine max
<400> 90
Met Glu Pro Ala Thr Asn Asn Asn Pro Ser Pro Ser Ala His Ala Ser
1 5 10 15
Leu His Glu Thr Lys Tyr Ile Glu Arg Val Lys His Ala Leu Ile Asp
20 25 30
Phe Gly Val Pro Glu Glu Leu Arg Leu Ser Asp Arg Ser Ile Tyr Lys
35 40 45
Val Pro Cys Tyr Leu Arg Lys Val Lys Glu Asp Ala Tyr Thr Pro Gln
50 55 60
Cys Ile Ser Ile Gly Pro Ile His Phe Lys Lys Glu Glu Leu Lys Pro
65 70 75 80
Met Gln Glu His Lys Leu Arg Tyr Tyr Gln Phe Phe Gly Arg Arg Val
85 90 95
Gly Val Ser Asp Glu Gln Met Glu Ala Tyr Lys His Tyr Leu Glu Thr
100 105 110
Glu Glu Lys Gln Ile Arg Gln Cys Tyr Ala Glu Lys Phe Leu Asp Ile
115 120 125
Thr Lys Asp Thr Phe Val Asp Met Met Leu Leu Asp Ala Val Phe Ile
130 135 140
Met Glu Leu Met Leu Arg Asn Cys Glu Phe Lys Ser His Lys Ala Lys
145 150 155 160
His Glu Gln Asn His Lys Arg Thr Glu Ser Phe Arg Ile Lys Asn Asn
165 170 175
Asn Asp Leu Ile Met Thr His Ser Trp Leu Ser Arg Asn Ile Ala Gly
180 185 190
Asp Leu Ile Leu Ile Glu Asn Gln Ile Pro Phe Phe Val Leu Gln Lys
195 200 205
Leu Tyr Asp Asp Val Val Pro Arg Glu Ser Lys Lys Asp Glu His Thr
210 215 220
Ala Gly Phe Val Lys Leu Ala Thr Glu Tyr Phe Ala Phe Tyr Asp Thr
225 230 235 240
Gln Met Ser Ser Ser Gly Glu Thr Lys Lys His Cys Ser Cys Tyr Ile
245 250 255
Leu His Cys Leu Lys Glu Pro Cys Lys Ser Lys Gly Lys Asp Arg Ser
260 265 270
Glu Ile Ser Lys Arg Pro Leu Gly Ser Asn Ser Glu Glu Asn Pro Glu
275 280 285
Gly Pro Lys His Phe Thr Asp Leu Ile Arg Trp Gln Phe Tyr Leu Pro
290 295 300
Thr Glu Cys Glu Ala Gly His Ala His Gln Val Leu Arg Thr Ala Thr
305 310 315 320
Lys Leu Gln Gly Ser Gly Ile Ser Phe Glu Lys Gly Asp Val Asn Lys
325 330 335
Arg Leu Leu Glu Ile Ala Phe Lys Lys Thr Pro Ile Leu Ser Ser Phe
340 345 350
Leu Cys Phe Gly Cys Phe Pro Leu Ser Lys Leu Phe Lys Ala Arg Leu
355 360 365
Arg Ile Pro Gln Leu Lys Val Asp His Thr Thr Glu Arg Val Phe Lys
370 375 380
Asn Leu Val Ala Phe Glu Gln Phe His Tyr Pro Asp Lys Pro Tyr Phe
385 390 395 400
Cys Asn Tyr Val Ser Phe Ile Asp Ser Leu Ile His Thr Gln Leu Asp
405 410 415
Val Glu Leu Leu Val Glu Lys Glu Val Ile Val His Glu Leu Gly Ser
420 425 430
Asp Lys Glu Val Ala Thr Leu Val Asn Gly Leu Cys Lys His Val Val
435 440 445
Thr Asn Ser Thr Cys Tyr His His Ile Ile Asn Lys Leu Asn Asp His
450 455 460
Tyr Met Asn Asp Trp Asn His Thr Ile Ala Ala Leu Arg Leu Val Tyr
465 470 475 480
Phe Arg Asp Leu Trp Arg Ala Ser Gly Thr Val Val Gly Ile Ala Val
485 490 495
Leu Val Phe Ala Val Phe Gln Phe Leu Arg Val Cys Arg Tyr Leu Phe
500 505 510
<210> 91
<211> 1713
<212> DNA
<213> Oryza sativa
<400> 91
atggcggcgg cggcggctga gatcgtgggg tccgcggcgg cgcgcatggc ggcgccggca 60
gtgaggccgg ctccgcccgc ggcggcggcg gcggcggcgc ccccgcagcc gaggagggcc 120
gtggcggcgc gctccctcag gacctccacc tccgacaggg tggcggcgga tctcgcgctc 180
gggagcaacg gctccctctc cgctcagaac attgctgaga ataccgctga cgctacttcg 240
caagtggtct ctgcgaattc ccgtaggaag acaaagattg tttgcaccat aggcccctca 300
accaacacac gcgagatgat atggaaactt gctgagactg gaatgaacgt cgcacgcatg 360
aatatgtccc atggtgacca ccagtcgcac cagaaggtga ttgatttggt gaaggagtac 420
aatgcgaaga atactgatgg caatgtcatt gctattatgc tggataccaa gggtccagaa 480
gtgagaagtg gggatgttcc agaaccaatc atgctcgagg aaggtcaaga gttcaatttt 540
actattaaaa gaggggtgag caccaaagac accgtcagtg tgaattacga tgacttcata 600
aacgatgttg aagttgggga catactgttg gtggatggag gaatgatgtc actcgctgtc 660
aagtctaaaa cagctgatac ggttaagtgt gaagtagttg atggtgggga actgaaatca 720
aggcgccacc taaatgtccg tggaaagagt gcgactttgc catctattac agagaaagat 780
tgggaggaca taaagtttgg tgttgaaaat ggtgttgatt tctatgccgt ttcctttgtg 840
aaggatgcaa aagttattca tgaactgaag gactacctta aaagtgctaa tgcagatata 900
catgtcattc caaagattga aagtgcagat tcaataccaa acctccagtc cattattgct 960
gcttcagatg gggcgatggt ggcaagaggg gatcttggtg ctgaacttcc aattgaggaa 1020
gttcctttgc tgcaggagga gattgtcaga acatgccgaa gcatgcagaa accagttatt 1080
gttgccacga atatgttaga gagcatgata gaccatccca ctccaacaag agcagaagtt 1140
tctgatatag caattgcagt tcgtgaaggt tctgatgcca tcatgctgtc tggtgaaact 1200
gcccatggaa agttcccact gaaggcagtc aaggtgatgc acacagtggc acagagaaca 1260
gaatccagcc tgtataaccc aactacatct cctagtcttg ttgcacatcc tcaggctctg 1320
ctcaacgagg aattttcgca aagccaacta agcaaaatgt ttggatctca tgctacaatg 1380
atggcgaaca ccctttgcac cccaattatt gtgtttacac gaaccggctc catggcagtc 1440
cttctcagcc actaccggcc ctcgtctaca atttttgcat ttacaaatga ggaacgagtg 1500
aagcaacgtc tggcactcta ccagggtgtg gttcccattt acatgaagtt ttctgatgat 1560
gcagaggaaa ctttctctag agcaattagt agcttgctga acgcccaatt cgtgaaagaa 1620
ggggactacg tgacccttgt tcagagtgga gtgaagtcga tctggagaga ggagtctact 1680
caccacattc aagtgaggaa agtccagggc taa 1713
<210> 92
<211> 570
<212> PRT
<213> Oryza sativa
<400> 92
Met Ala Ala Ala Ala Ala Glu Ile Val Gly Ser Ala Ala Ala Arg Met
1 5 10 15
Ala Ala Pro Ala Val Arg Pro Ala Pro Pro Ala Ala Ala Ala Ala Ala
20 25 30
Ala Pro Pro Gln Pro Arg Arg Ala Val Ala Ala Arg Ser Leu Arg Thr
35 40 45
Ser Thr Ser Asp Arg Val Ala Ala Asp Leu Ala Leu Gly Ser Asn Gly
50 55 60
Ser Leu Ser Ala Gln Asn Ile Ala Glu Asn Thr Ala Asp Ala Thr Ser
65 70 75 80
Gln Val Val Ser Ala Asn Ser Arg Arg Lys Thr Lys Ile Val Cys Thr
85 90 95
Ile Gly Pro Ser Thr Asn Thr Arg Glu Met Ile Trp Lys Leu Ala Glu
100 105 110
Thr Gly Met Asn Val Ala Arg Met Asn Met Ser His Gly Asp His Gln
115 120 125
Ser His Gln Lys Val Ile Asp Leu Val Lys Glu Tyr Asn Ala Lys Asn
130 135 140
Thr Asp Gly Asn Val Ile Ala Ile Met Leu Asp Thr Lys Gly Pro Glu
145 150 155 160
Val Arg Ser Gly Asp Val Pro Glu Pro Ile Met Leu Glu Glu Gly Gln
165 170 175
Glu Phe Asn Phe Thr Ile Lys Arg Gly Val Ser Thr Lys Asp Thr Val
180 185 190
Ser Val Asn Tyr Asp Asp Phe Ile Asn Asp Val Glu Val Gly Asp Ile
195 200 205
Leu Leu Val Asp Gly Gly Met Met Ser Leu Ala Val Lys Ser Lys Thr
210 215 220
Ala Asp Thr Val Lys Cys Glu Val Val Asp Gly Gly Glu Leu Lys Ser
225 230 235 240
Arg Arg His Leu Asn Val Arg Gly Lys Ser Ala Thr Leu Pro Ser Ile
245 250 255
Thr Glu Lys Asp Trp Glu Asp Ile Lys Phe Gly Val Glu Asn Gly Val
260 265 270
Asp Phe Tyr Ala Val Ser Phe Val Lys Asp Ala Lys Val Ile His Glu
275 280 285
Leu Lys Asp Tyr Leu Lys Ser Ala Asn Ala Asp Ile His Val Ile Pro
290 295 300
Lys Ile Glu Ser Ala Asp Ser Ile Pro Asn Leu Gln Ser Ile Ile Ala
305 310 315 320
Ala Ser Asp Gly Ala Met Val Ala Arg Gly Asp Leu Gly Ala Glu Leu
325 330 335
Pro Ile Glu Glu Val Pro Leu Leu Gln Glu Glu Ile Val Arg Thr Cys
340 345 350
Arg Ser Met Gln Lys Pro Val Ile Val Ala Thr Asn Met Leu Glu Ser
355 360 365
Met Ile Asp His Pro Thr Pro Thr Arg Ala Glu Val Ser Asp Ile Ala
370 375 380
Ile Ala Val Arg Glu Gly Ser Asp Ala Ile Met Leu Ser Gly Glu Thr
385 390 395 400
Ala His Gly Lys Phe Pro Leu Lys Ala Val Lys Val Met His Thr Val
405 410 415
Ala Gln Arg Thr Glu Ser Ser Leu Tyr Asn Pro Thr Thr Ser Pro Ser
420 425 430
Leu Val Ala His Pro Gln Ala Leu Leu Asn Glu Glu Phe Ser Gln Ser
435 440 445
Gln Leu Ser Lys Met Phe Gly Ser His Ala Thr Met Met Ala Asn Thr
450 455 460
Leu Cys Thr Pro Ile Ile Val Phe Thr Arg Thr Gly Ser Met Ala Val
465 470 475 480
Leu Leu Ser His Tyr Arg Pro Ser Ser Thr Ile Phe Ala Phe Thr Asn
485 490 495
Glu Glu Arg Val Lys Gln Arg Leu Ala Leu Tyr Gln Gly Val Val Pro
500 505 510
Ile Tyr Met Lys Phe Ser Asp Asp Ala Glu Glu Thr Phe Ser Arg Ala
515 520 525
Ile Ser Ser Leu Leu Asn Ala Gln Phe Val Lys Glu Gly Asp Tyr Val
530 535 540
Thr Leu Val Gln Ser Gly Val Lys Ser Ile Trp Arg Glu Glu Ser Thr
545 550 555 560
His His Ile Gln Val Arg Lys Val Gln Gly
565 570
<210> 93
<211> 1728
<212> DNA
<213> Zea mays
<400> 93
atggcggcgc aggtggtcgc agcggcgggc accccggtgg ccaggccgct gggcggtgga 60
tctggggccg acgcgctccg gcccgcggcg aggatgccaa gggagagaag gaccggtgct 120
gtggccgcga gaggacgccg cgagtctcag gtggtatccg tcataagccg cgccccacgc 180
cccgatgccg gggtgctgcc ggtgtcgccc gacgacgacg cggccgtaaa ggaagaagca 240
aacttccagc accttaaggc tatccagcaa cttgcaactg cagcaaatgg cgtgtggtct 300
aaaccaaatg taaggcgcaa gacaaagatt gtgtgtacaa tcggtccttc aaccaacaca 360
agggacatga tatggaaact cgctgagact ggcatgaatg tggctcggct taatatgtca 420
catggagacc atgcatcaca ccagaaagtt attgatctgg taaaggagta caatgcatca 480
catgctgaca atgtgattgc tatcatgctt gacacaaagg gaccagaagt tcgaagtgga 540
gatttgcctc aaccgatatt tctggaaagc ggacaagaat ttacttttac aatcaaaagg 600
ggagttggga ctgatacgtg tgttagcgtt aactatgacg actttgttaa tgatgttgaa 660
gtgggcgaca tgctccttgt agatggagga atgatgtcat tcttggtcaa atcaaaaact 720
gaagattctg tgaaatgtga agttattgac ggtggtgaat tgaaatctag gcgccatctg 780
aatgttcgtg gaaagagtgc aaccttgcca tcaataactg acaaggactg ggacgacatt 840
aagtttggtg tggataatca agttgattac tatgctgttt cctttgtgaa agatgctcaa 900
gttgtccacg aactcaagga ttatctaaaa agttgcaatg ctgacataca tgttatcgta 960
aaaatagaaa gtgcagactc catccccaac ttacattcaa tcatcacagc atctgatggg 1020
gctatggttg ccaggggtga ccttggagcc gagctgccca ttgaggaggt gccgctgttg 1080
caggaagaaa ttattagaat gtgcaggagc atggggaagg ctgttattgt tgctacaaat 1140
atgctcgaaa gtatgattgt tcatccaact ccaacccgag cagaagtttc agacattgct 1200
atagctgtcc gagagggtgc tgatgcagtt atgctttcag gagaaactgc acatgggaaa 1260
tttcccttga aagctgttaa ggtcatgcac actgttgccc tgagaaccga ggcaactatt 1320
cctggtgggg aaacacctgc agaccttggt caggctttca agaaccacat gagcgaaatg 1380
ttcgcatacc atgcaacaat gatgtcaaat acccttcgaa catcaatagt ggttttcact 1440
aggacgggat ttatggctat actgcttagt cactaccgtc catctggcac tatttttgcc 1500
tttacagatg aggagagggt taggcaacga ttggctttgt accaaggtgt atgtccggtt 1560
caaatggaat tttctgatga tgctgagaag acatttggca atgctttgtc ttatttgctg 1620
aaacatggta tggttaagga cggtgaggag gttgcgctcg ttcaaagtgg taaacatccc 1680
atctggagat cacaatcaac acacaacatt caggtgagga agatctga 1728
<210> 94
<211> 575
<212> PRT
<213> Zea mays
<400> 94
Met Ala Ala Gln Val Val Ala Ala Ala Gly Thr Pro Val Ala Arg Pro
1 5 10 15
Leu Gly Gly Gly Ser Gly Ala Asp Ala Leu Arg Pro Ala Ala Arg Met
20 25 30
Pro Arg Glu Arg Arg Thr Gly Ala Val Ala Ala Arg Gly Arg Arg Glu
35 40 45
Ser Gln Val Val Ser Val Ile Ser Arg Ala Pro Arg Pro Asp Ala Gly
50 55 60
Val Leu Pro Val Ser Pro Asp Asp Asp Ala Ala Val Lys Glu Glu Ala
65 70 75 80
Asn Phe Gln His Leu Lys Ala Ile Gln Gln Leu Ala Thr Ala Ala Asn
85 90 95
Gly Val Trp Ser Lys Pro Asn Val Arg Arg Lys Thr Lys Ile Val Cys
100 105 110
Thr Ile Gly Pro Ser Thr Asn Thr Arg Asp Met Ile Trp Lys Leu Ala
115 120 125
Glu Thr Gly Met Asn Val Ala Arg Leu Asn Met Ser His Gly Asp His
130 135 140
Ala Ser His Gln Lys Val Ile Asp Leu Val Lys Glu Tyr Asn Ala Ser
145 150 155 160
His Ala Asp Asn Val Ile Ala Ile Met Leu Asp Thr Lys Gly Pro Glu
165 170 175
Val Arg Ser Gly Asp Leu Pro Gln Pro Ile Phe Leu Glu Ser Gly Gln
180 185 190
Glu Phe Thr Phe Thr Ile Lys Arg Gly Val Gly Thr Asp Thr Cys Val
195 200 205
Ser Val Asn Tyr Asp Asp Phe Val Asn Asp Val Glu Val Gly Asp Met
210 215 220
Leu Leu Val Asp Gly Gly Met Met Ser Phe Leu Val Lys Ser Lys Thr
225 230 235 240
Glu Asp Ser Val Lys Cys Glu Val Ile Asp Gly Gly Glu Leu Lys Ser
245 250 255
Arg Arg His Leu Asn Val Arg Gly Lys Ser Ala Thr Leu Pro Ser Ile
260 265 270
Thr Asp Lys Asp Trp Asp Asp Ile Lys Phe Gly Val Asp Asn Gln Val
275 280 285
Asp Tyr Tyr Ala Val Ser Phe Val Lys Asp Ala Gln Val Val His Glu
290 295 300
Leu Lys Asp Tyr Leu Lys Ser Cys Asn Ala Asp Ile His Val Ile Val
305 310 315 320
Lys Ile Glu Ser Ala Asp Ser Ile Pro Asn Leu His Ser Ile Ile Thr
325 330 335
Ala Ser Asp Gly Ala Met Val Ala Arg Gly Asp Leu Gly Ala Glu Leu
340 345 350
Pro Ile Glu Glu Val Pro Leu Leu Gln Glu Glu Ile Ile Arg Met Cys
355 360 365
Arg Ser Met Gly Lys Ala Val Ile Val Ala Thr Asn Met Leu Glu Ser
370 375 380
Met Ile Val His Pro Thr Pro Thr Arg Ala Glu Val Ser Asp Ile Ala
385 390 395 400
Ile Ala Val Arg Glu Gly Ala Asp Ala Val Met Leu Ser Gly Glu Thr
405 410 415
Ala His Gly Lys Phe Pro Leu Lys Ala Val Lys Val Met His Thr Val
420 425 430
Ala Leu Arg Thr Glu Ala Thr Ile Pro Gly Gly Glu Thr Pro Ala Asp
435 440 445
Leu Gly Gln Ala Phe Lys Asn His Met Ser Glu Met Phe Ala Tyr His
450 455 460
Ala Thr Met Met Ser Asn Thr Leu Arg Thr Ser Ile Val Val Phe Thr
465 470 475 480
Arg Thr Gly Phe Met Ala Ile Leu Leu Ser His Tyr Arg Pro Ser Gly
485 490 495
Thr Ile Phe Ala Phe Thr Asp Glu Glu Arg Val Arg Gln Arg Leu Ala
500 505 510
Leu Tyr Gln Gly Val Cys Pro Val Gln Met Glu Phe Ser Asp Asp Ala
515 520 525
Glu Lys Thr Phe Gly Asn Ala Leu Ser Tyr Leu Leu Lys His Gly Met
530 535 540
Val Lys Asp Gly Glu Glu Val Ala Leu Val Gln Ser Gly Lys His Pro
545 550 555 560
Ile Trp Arg Ser Gln Ser Thr His Asn Ile Gln Val Arg Lys Ile
565 570 575
<210> 95
<211> 1743
<212> DNA
<213> Sorghum bicolor
<400> 95
atggcggcgc aggtggtggc agcggcgggc accgcggtgg ccaggccgct gggcggcgga 60
tctgggctcg acgcggcgcc ccgtggcggc ggcgtggcga ggatgccaag ggagaggtgg 120
ggcggcgctg tggccgcgag gggacgccgc gaccgcgagt cgcaggtggt gtccgtcata 180
agctgcgccc cgcgctccca ggccgaggtg ctgccggtgt cgcccgacga cgacgcggcc 240
gtaaaggaag aagcaaactt ccagcacctt aaggctatcc agcagcttgc aactgcagca 300
aatggtgtgt ggtctaaacc aaatgtaagg cgcaagacaa agatcgtgtg tacaattggt 360
ccttcaacta acacaaggga gatgatatgg aaacttgccg aggctggcat gaatgtggct 420
cggcttaata tgtcacatgg agaccatgcg tcacaccaga aagttattga tctggtgaag 480
gagtataatg catcacatgc tgacaatgtg attgctatca tgcttgacac aaagggacca 540
gaagttcgaa gtggagattt gcctcaaccg atatttctgg aaagtgggca agagttcact 600
tttacaatca aaaggggagt tgggactgat acatgtgtta gcgttaacta tgatgacttt 660
gttaatgatg ttgaagttgg tgacatgctc cttgtagacg gaggaatgat gtcattcttg 720
gtcaaatcaa aaactgaaga ttctgtgaaa tgtgaagtta ttgatggtgg tgaattgaaa 780
tctaggcgcc atctgaatgt tcgtggaaag agtgcaacct tgccatcaat aactgacaag 840
gattgggacg acattaagtt tggtgtggat aatcaagttg attactatgc tgtttctttt 900
gtgaaagatg ctcaagttgt ccatgaactc aaggattatc taagaagttg caatgccgac 960
atacatgtaa ttgtaaaaat cgaaagtgcg gactccatcc caaacctaca ttcaatcatc 1020
acagcatctg atggggctat ggttgccagg ggtgaccttg gagctgaact gcccatcgag 1080
gaggtgccgc tgttgcagga agaaattatt agaatgtgta ggagcatggg gaaggctgtt 1140
attgtcgcta caaatatgct ggaaagtatg attgttcatc caactccaac ccgagcagaa 1200
gtttcagaca ttgctatagc tgtccgagag ggtgctgatg cagtcatgct ttctggagaa 1260
actgcacatg ggaaatttcc cttgaaagct gttaaggtca tgcacactgt tgccctgaga 1320
actgaggcaa ctattcctgg tggagaaaca cctgcagacc ttggtcaggc tttcaagaac 1380
cacatgagtg aaatgtttgc gtaccatgca acaatgatgt caaataccct tcgaacatca 1440
atagtggttt tcactaggac aggatttatg gctatactgc ttagtcacta ccgtccatct 1500
ggcactattt ttgccttcac agatgaggag agagttaggc aacgattggc tttgtaccaa 1560
ggtgtatgtc caattcaaat ggaattttct gatgatgctg agaagacatt tggcaacgct 1620
ttgtcttatt tgctgaaaca tggtatggtt aaggatggtg aggaggtcgc actccttcaa 1680
agtggtaatc agcccatctg gagatcacaa tcaacacaca acattcaggt gaggaagatc 1740
tga 1743
<210> 96
<211> 580
<212> PRT
<213> Sorghum bicolor
<400> 96
Met Ala Ala Gln Val Val Ala Ala Ala Gly Thr Ala Val Ala Arg Pro
1 5 10 15
Leu Gly Gly Gly Ser Gly Leu Asp Ala Ala Pro Arg Gly Gly Gly Val
20 25 30
Ala Arg Met Pro Arg Glu Arg Trp Gly Gly Ala Val Ala Ala Arg Gly
35 40 45
Arg Arg Asp Arg Glu Ser Gln Val Val Ser Val Ile Ser Cys Ala Pro
50 55 60
Arg Ser Gln Ala Glu Val Leu Pro Val Ser Pro Asp Asp Asp Ala Ala
65 70 75 80
Val Lys Glu Glu Ala Asn Phe Gln His Leu Lys Ala Ile Gln Gln Leu
85 90 95
Ala Thr Ala Ala Asn Gly Val Trp Ser Lys Pro Asn Val Arg Arg Lys
100 105 110
Thr Lys Ile Val Cys Thr Ile Gly Pro Ser Thr Asn Thr Arg Glu Met
115 120 125
Ile Trp Lys Leu Ala Glu Ala Gly Met Asn Val Ala Arg Leu Asn Met
130 135 140
Ser His Gly Asp His Ala Ser His Gln Lys Val Ile Asp Leu Val Lys
145 150 155 160
Glu Tyr Asn Ala Ser His Ala Asp Asn Val Ile Ala Ile Met Leu Asp
165 170 175
Thr Lys Gly Pro Glu Val Arg Ser Gly Asp Leu Pro Gln Pro Ile Phe
180 185 190
Leu Glu Ser Gly Gln Glu Phe Thr Phe Thr Ile Lys Arg Gly Val Gly
195 200 205
Thr Asp Thr Cys Val Ser Val Asn Tyr Asp Asp Phe Val Asn Asp Val
210 215 220
Glu Val Gly Asp Met Leu Leu Val Asp Gly Gly Met Met Ser Phe Leu
225 230 235 240
Val Lys Ser Lys Thr Glu Asp Ser Val Lys Cys Glu Val Ile Asp Gly
245 250 255
Gly Glu Leu Lys Ser Arg Arg His Leu Asn Val Arg Gly Lys Ser Ala
260 265 270
Thr Leu Pro Ser Ile Thr Asp Lys Asp Trp Asp Asp Ile Lys Phe Gly
275 280 285
Val Asp Asn Gln Val Asp Tyr Tyr Ala Val Ser Phe Val Lys Asp Ala
290 295 300
Gln Val Val His Glu Leu Lys Asp Tyr Leu Arg Ser Cys Asn Ala Asp
305 310 315 320
Ile His Val Ile Val Lys Ile Glu Ser Ala Asp Ser Ile Pro Asn Leu
325 330 335
His Ser Ile Ile Thr Ala Ser Asp Gly Ala Met Val Ala Arg Gly Asp
340 345 350
Leu Gly Ala Glu Leu Pro Ile Glu Glu Val Pro Leu Leu Gln Glu Glu
355 360 365
Ile Ile Arg Met Cys Arg Ser Met Gly Lys Ala Val Ile Val Ala Thr
370 375 380
Asn Met Leu Glu Ser Met Ile Val His Pro Thr Pro Thr Arg Ala Glu
385 390 395 400
Val Ser Asp Ile Ala Ile Ala Val Arg Glu Gly Ala Asp Ala Val Met
405 410 415
Leu Ser Gly Glu Thr Ala His Gly Lys Phe Pro Leu Lys Ala Val Lys
420 425 430
Val Met His Thr Val Ala Leu Arg Thr Glu Ala Thr Ile Pro Gly Gly
435 440 445
Glu Thr Pro Ala Asp Leu Gly Gln Ala Phe Lys Asn His Met Ser Glu
450 455 460
Met Phe Ala Tyr His Ala Thr Met Met Ser Asn Thr Leu Arg Thr Ser
465 470 475 480
Ile Val Val Phe Thr Arg Thr Gly Phe Met Ala Ile Leu Leu Ser His
485 490 495
Tyr Arg Pro Ser Gly Thr Ile Phe Ala Phe Thr Asp Glu Glu Arg Val
500 505 510
Arg Gln Arg Leu Ala Leu Tyr Gln Gly Val Cys Pro Ile Gln Met Glu
515 520 525
Phe Ser Asp Asp Ala Glu Lys Thr Phe Gly Asn Ala Leu Ser Tyr Leu
530 535 540
Leu Lys His Gly Met Val Lys Asp Gly Glu Glu Val Ala Leu Leu Gln
545 550 555 560
Ser Gly Asn Gln Pro Ile Trp Arg Ser Gln Ser Thr His Asn Ile Gln
565 570 575
Val Arg Lys Ile
580
<210> 97
<211> 1740
<212> DNA
<213> Arabidopsis thaliana
<400> 97
atggctcaag tggttgctac caggtcaatt caaggctcga tgttatctcc caacggtgga 60
tctgtgtcta caagatccga gaagctattg aaaccagcga gttttgcagt gaaggttctt 120
ggcaacgaag caaagagaag tggaagagtc tctgtaagaa gcagaagagt ggttgatact 180
actgtgagat ccgctcgtgt tgagactgaa gtcattcctg tttctcctga agatgtgcct 240
aacagagagg agcagcttga gaggttgttg gaaatgcagc agtttggtga tacatcggta 300
gggatgtggt cgaagccgac agtgaggagg aagacaaaga ttgtttgcac cgttggtccg 360
tcgaccaaca cacgagaaat gatatggaaa ttggctgaag ctgggatgaa tgttgctagg 420
atgaatatgt ctcatggaga tcatgcttca cataagaagg ttattgattt ggttaaagaa 480
tacaatgcac aaactaaaga caacactatt gctatcatgc ttgacaccaa gggtccggaa 540
gttaggagtg gagatttacc tcagccaatt atgttagatc ctggtcaaga gtttaccttt 600
acaattgaga gaggagtcag cacaccaagt tgtgtcagtg ttaactatga tgatttcgtt 660
aatgacgtgg aagcgggtga catgcttctt gttgatggtg gtatgatgtc gtttatggtg 720
aagtcaaaga ccaaagactc tgtcaaatgt gaagttgttg atggtggaga acttaagtca 780
aggagacacc tgaatgtccg aggaaagagt gcaactttac cttcaatcac tgagaaggac 840
tgggaggata ttaaatttgg agtggagaac aaagttgact tttatgcagt ttcctttgtc 900
aaagatgctc aagttgtaca cgagttgaag aaataccttc aaaatagtgg tgctgatata 960
cacgtgatag tgaaaattga gagtgcagac tccataccta acttgcactc cattatcaca 1020
gcatcagatg gggcaatggt tgcaagaggt gatcttggtg cagagcttcc aattgaagaa 1080
gtccccattc ttcaggagga gatcattaac ctgtgccgta gtatgggaaa agctgttatt 1140
gttgcgacta acatgcttga gagtatgata gttcatccaa ctccaacccg ggcagaggtc 1200
tcagacattg ctatcgctgt tagagaaggt gctgatgcgg taatgctttc aggagaaact 1260
gctcacggaa agttcccatt gaaagctgct ggagtgatgc acactgttgc attgcgaaca 1320
gaagcaacca ttactagcgg tgaaatgcca cctaatcttg gtcaagcctt caagaaccat 1380
atgagtgaga tgtttgcata ccatgcaacc atgatgtcaa acacacttgg aacttcaact 1440
gttgtcttca ccagaaccgg tttcatggcc atattgttaa gtcactatcg tccttccggc 1500
acaatctatg ccttcacaaa tgagaaaaaa atacaacaaa gattagcttt gtatcaaggt 1560
gtatgcccca tatatatgga gttcacagat gatgcagaag aaacttttgc taatgctttg 1620
gctacattac tgaaacaagg aatggtgaag aagggagagg aaatagcaat cgtacagagc 1680
ggtacacagc caatctggcg atctcaatcg acacataaca tccaagtccg caaggtttaa 1740
<210> 98
<211> 579
<212> PRT
<213> Arabidopsis thaliana
<400> 98
Met Ala Gln Val Val Ala Thr Arg Ser Ile Gln Gly Ser Met Leu Ser
1 5 10 15
Pro Asn Gly Gly Ser Val Ser Thr Arg Ser Glu Lys Leu Leu Lys Pro
20 25 30
Ala Ser Phe Ala Val Lys Val Leu Gly Asn Glu Ala Lys Arg Ser Gly
35 40 45
Arg Val Ser Val Arg Ser Arg Arg Val Val Asp Thr Thr Val Arg Ser
50 55 60
Ala Arg Val Glu Thr Glu Val Ile Pro Val Ser Pro Glu Asp Val Pro
65 70 75 80
Asn Arg Glu Glu Gln Leu Glu Arg Leu Leu Glu Met Gln Gln Phe Gly
85 90 95
Asp Thr Ser Val Gly Met Trp Ser Lys Pro Thr Val Arg Arg Lys Thr
100 105 110
Lys Ile Val Cys Thr Val Gly Pro Ser Thr Asn Thr Arg Glu Met Ile
115 120 125
Trp Lys Leu Ala Glu Ala Gly Met Asn Val Ala Arg Met Asn Met Ser
130 135 140
His Gly Asp His Ala Ser His Lys Lys Val Ile Asp Leu Val Lys Glu
145 150 155 160
Tyr Asn Ala Gln Thr Lys Asp Asn Thr Ile Ala Ile Met Leu Asp Thr
165 170 175
Lys Gly Pro Glu Val Arg Ser Gly Asp Leu Pro Gln Pro Ile Met Leu
180 185 190
Asp Pro Gly Gln Glu Phe Thr Phe Thr Ile Glu Arg Gly Val Ser Thr
195 200 205
Pro Ser Cys Val Ser Val Asn Tyr Asp Asp Phe Val Asn Asp Val Glu
210 215 220
Ala Gly Asp Met Leu Leu Val Asp Gly Gly Met Met Ser Phe Met Val
225 230 235 240
Lys Ser Lys Thr Lys Asp Ser Val Lys Cys Glu Val Val Asp Gly Gly
245 250 255
Glu Leu Lys Ser Arg Arg His Leu Asn Val Arg Gly Lys Ser Ala Thr
260 265 270
Leu Pro Ser Ile Thr Glu Lys Asp Trp Glu Asp Ile Lys Phe Gly Val
275 280 285
Glu Asn Lys Val Asp Phe Tyr Ala Val Ser Phe Val Lys Asp Ala Gln
290 295 300
Val Val His Glu Leu Lys Lys Tyr Leu Gln Asn Ser Gly Ala Asp Ile
305 310 315 320
His Val Ile Val Lys Ile Glu Ser Ala Asp Ser Ile Pro Asn Leu His
325 330 335
Ser Ile Ile Thr Ala Ser Asp Gly Ala Met Val Ala Arg Gly Asp Leu
340 345 350
Gly Ala Glu Leu Pro Ile Glu Glu Val Pro Ile Leu Gln Glu Glu Ile
355 360 365
Ile Asn Leu Cys Arg Ser Met Gly Lys Ala Val Ile Val Ala Thr Asn
370 375 380
Met Leu Glu Ser Met Ile Val His Pro Thr Pro Thr Arg Ala Glu Val
385 390 395 400
Ser Asp Ile Ala Ile Ala Val Arg Glu Gly Ala Asp Ala Val Met Leu
405 410 415
Ser Gly Glu Thr Ala His Gly Lys Phe Pro Leu Lys Ala Ala Gly Val
420 425 430
Met His Thr Val Ala Leu Arg Thr Glu Ala Thr Ile Thr Ser Gly Glu
435 440 445
Met Pro Pro Asn Leu Gly Gln Ala Phe Lys Asn His Met Ser Glu Met
450 455 460
Phe Ala Tyr His Ala Thr Met Met Ser Asn Thr Leu Gly Thr Ser Thr
465 470 475 480
Val Val Phe Thr Arg Thr Gly Phe Met Ala Ile Leu Leu Ser His Tyr
485 490 495
Arg Pro Ser Gly Thr Ile Tyr Ala Phe Thr Asn Glu Lys Lys Ile Gln
500 505 510
Gln Arg Leu Ala Leu Tyr Gln Gly Val Cys Pro Ile Tyr Met Glu Phe
515 520 525
Thr Asp Asp Ala Glu Glu Thr Phe Ala Asn Ala Leu Ala Thr Leu Leu
530 535 540
Lys Gln Gly Met Val Lys Lys Gly Glu Glu Ile Ala Ile Val Gln Ser
545 550 555 560
Gly Thr Gln Pro Ile Trp Arg Ser Gln Ser Thr His Asn Ile Gln Val
565 570 575
Arg Lys Val
<210> 99
<211> 1728
<212> DNA
<213> Glycine max
<400> 99
atgtctcagg tagtggccac tcgatccatt cactcctccc tcacgcgccc cacctcagga 60
tctgcacacc acagggccca aacgttgttg aagcctccaa cttttgcttc caaattgttc 120
ggagcacaaa ggaacaaccc ctccaaagtt tgctcccgaa gttgcctcgt caatgcgagg 180
aaatctgcac ccgctaaagt tgttcccgtg tcacccgagg atgattcaaa gattgaggaa 240
gagttgcagc acttgcgtgg tatgcagcaa cttggcgaca cttctgttgg aatgtggtca 300
aaacccacgt ttaggaggaa gacaaaggtt gtttgcacca ttggtccttc taccaacacc 360
agggaaatga tttggaagct ggctgaggct gggatgaatg ttgcccgatt gaatatgtct 420
cacggagacc atgcttctca tcagaaaatt attgatttgg ttaaagaata taatgctcaa 480
tccaaggaca acgtaattgc aattatgctt gataccaagg gtcctgaggt taggagtggg 540
gatttgccac aaccaatcaa tttaacaact gggcaggaat tcacttttac catccggagg 600
ggtgttggaa ctgcagattg tgttagtgtg aactatgacg atttcgtcaa tgatgtggat 660
gtgggagaca tgcttcttgt tgatggtggt atgatgtctt tggtggttaa gtctaagaca 720
gaggattctg tgaaatgtga agttgttgat ggaggagagc tcaagtcaag gagacatttg 780
aatgttagag gaaaaagtgc aacactgcct tccataactg agaaggattg ggatgacatc 840
aaatttggag tggataacaa agttgacttc tatgctgttt cttttgttaa ggatgcacaa 900
gtagttcatg aactgaagaa ttatttgaaa agctgtgatg ctgatataca cgtcattgta 960
aaaattgaaa gtgcagactc tataccaaac ttgcattcaa ttattacagc gtctgatggg 1020
gccatggttg caagaggaga tcttggtgca gaactcccta ttgaagaggt tccacttttg 1080
caggaagaaa taatcaccat atgtcgtagc atgggaaagg ccgttattgt ggcaacaaat 1140
atgctggaaa gcatgattgt tcacccgaca ccaaccagag ccgaggtatc cgatattgca 1200
attgctgttc gagaaggttc tgatgcaata atgctttctg gggaaactgc tcatggaaag 1260
ttcccactaa aagccgtgaa agtaatgcac accgtagcat tacggacaga agccactata 1320
cctggtggtc aaatgccacc aaatattggt caagtattca agaaccacat gagtgagatg 1380
tttgcttacc atgcaaccat gatgtctaat acccttggaa cctcaactgt tgtcttcact 1440
agatcaggct tcatggctat ccttttgagc cactatcgac cttcaggcac catatttgct 1500
tttacagatc aaaagaggat acaacagagg ttggctttgt atcaaggagt ctgtcctatt 1560
tacatggaat tctctgaaga tgctgaagag actttcacaa gggccttgga tttgctgcag 1620
aagcaaggaa tggtgaaatc aggagaagaa gtagcactag tacaaagtgg cacgcaaccc 1680
atatggaggt tccaatccac tcacaatatc caggtccgaa cagtgtaa 1728
<210> 100
<211> 575
<212> PRT
<213> Glycine max
<400> 100
Met Ser Gln Val Val Ala Thr Arg Ser Ile His Ser Ser Leu Thr Arg
1 5 10 15
Pro Thr Ser Gly Ser Ala His His Arg Ala Gln Thr Leu Leu Lys Pro
20 25 30
Pro Thr Phe Ala Ser Lys Leu Phe Gly Ala Gln Arg Asn Asn Pro Ser
35 40 45
Lys Val Cys Ser Arg Ser Cys Leu Val Asn Ala Arg Lys Ser Ala Pro
50 55 60
Ala Lys Val Val Pro Val Ser Pro Glu Asp Asp Ser Lys Ile Glu Glu
65 70 75 80
Glu Leu Gln His Leu Arg Gly Met Gln Gln Leu Gly Asp Thr Ser Val
85 90 95
Gly Met Trp Ser Lys Pro Thr Phe Arg Arg Lys Thr Lys Val Val Cys
100 105 110
Thr Ile Gly Pro Ser Thr Asn Thr Arg Glu Met Ile Trp Lys Leu Ala
115 120 125
Glu Ala Gly Met Asn Val Ala Arg Leu Asn Met Ser His Gly Asp His
130 135 140
Ala Ser His Gln Lys Ile Ile Asp Leu Val Lys Glu Tyr Asn Ala Gln
145 150 155 160
Ser Lys Asp Asn Val Ile Ala Ile Met Leu Asp Thr Lys Gly Pro Glu
165 170 175
Val Arg Ser Gly Asp Leu Pro Gln Pro Ile Asn Leu Thr Thr Gly Gln
180 185 190
Glu Phe Thr Phe Thr Ile Arg Arg Gly Val Gly Thr Ala Asp Cys Val
195 200 205
Ser Val Asn Tyr Asp Asp Phe Val Asn Asp Val Asp Val Gly Asp Met
210 215 220
Leu Leu Val Asp Gly Gly Met Met Ser Leu Val Val Lys Ser Lys Thr
225 230 235 240
Glu Asp Ser Val Lys Cys Glu Val Val Asp Gly Gly Glu Leu Lys Ser
245 250 255
Arg Arg His Leu Asn Val Arg Gly Lys Ser Ala Thr Leu Pro Ser Ile
260 265 270
Thr Glu Lys Asp Trp Asp Asp Ile Lys Phe Gly Val Asp Asn Lys Val
275 280 285
Asp Phe Tyr Ala Val Ser Phe Val Lys Asp Ala Gln Val Val His Glu
290 295 300
Leu Lys Asn Tyr Leu Lys Ser Cys Asp Ala Asp Ile His Val Ile Val
305 310 315 320
Lys Ile Glu Ser Ala Asp Ser Ile Pro Asn Leu His Ser Ile Ile Thr
325 330 335
Ala Ser Asp Gly Ala Met Val Ala Arg Gly Asp Leu Gly Ala Glu Leu
340 345 350
Pro Ile Glu Glu Val Pro Leu Leu Gln Glu Glu Ile Ile Thr Ile Cys
355 360 365
Arg Ser Met Gly Lys Ala Val Ile Val Ala Thr Asn Met Leu Glu Ser
370 375 380
Met Ile Val His Pro Thr Pro Thr Arg Ala Glu Val Ser Asp Ile Ala
385 390 395 400
Ile Ala Val Arg Glu Gly Ser Asp Ala Ile Met Leu Ser Gly Glu Thr
405 410 415
Ala His Gly Lys Phe Pro Leu Lys Ala Val Lys Val Met His Thr Val
420 425 430
Ala Leu Arg Thr Glu Ala Thr Ile Pro Gly Gly Gln Met Pro Pro Asn
435 440 445
Ile Gly Gln Val Phe Lys Asn His Met Ser Glu Met Phe Ala Tyr His
450 455 460
Ala Thr Met Met Ser Asn Thr Leu Gly Thr Ser Thr Val Val Phe Thr
465 470 475 480
Arg Ser Gly Phe Met Ala Ile Leu Leu Ser His Tyr Arg Pro Ser Gly
485 490 495
Thr Ile Phe Ala Phe Thr Asp Gln Lys Arg Ile Gln Gln Arg Leu Ala
500 505 510
Leu Tyr Gln Gly Val Cys Pro Ile Tyr Met Glu Phe Ser Glu Asp Ala
515 520 525
Glu Glu Thr Phe Thr Arg Ala Leu Asp Leu Leu Gln Lys Gln Gly Met
530 535 540
Val Lys Ser Gly Glu Glu Val Ala Leu Val Gln Ser Gly Thr Gln Pro
545 550 555 560
Ile Trp Arg Phe Gln Ser Thr His Asn Ile Gln Val Arg Thr Val
565 570 575
<210> 101
<211> 1788
<212> DNA
<213> Oryza sativa
<400> 101
atggttgaaa gtgcaagcat ggtgaacgag aactcggaga atccatactg gaaagcaata 60
ggatacagag tggaagagcc ccgacgtgat cgagcagagt cgatgccgtc gccgtcgcca 120
tcgccggtat cgcggcggcc actggacaac ggcgtcgtgg agacgagggc gctgacggac 180
accaccctcc tccggtcgct cgcggcgaag ggcctcgccg tgaggcccgg cgcgtcggac 240
gagcaccaca cggtgcggtg cgacgccgtc atcgtcggct ccggctgcgg cggcggcgtg 300
gccgccgcgg tgctcgcgtc cgccgggtac aaggtggtcg tcgtcgagaa gggcgactac 360
ttcaccaagg aggattacag ctcgatcgag ggcccgtcca tggagcgcct cttcgagagg 420
ggcggcgtct tctgcacgtc caacgtcacg acgatgatat tcaccggcgc gacggtcggc 480
ggcgggtcgg cggtgaactg gtcggcgagc atccgcacgc cggcgggcgt gatgcaggag 540
tggtcgcgcg agcacgggct ggcggtgttc gcgagccccg ggtacgcgcg ggccatggac 600
gcggtgtgcg agcgcctcgg tgtgaccgac gcgtgccggg aggaagggtt ccagaacaag 660
gtggtgcgcc gcgggtgcga cgcgctcggg ctgcgcgccg acgccgtgcc gcgcaactcg 720
tcggaggggc acttctgcgg cagctgcaac ttcgggtgcc ccaccggcga caagaagggc 780
accgacacga cgtggctcgt cgacgccgtc gagcgcggtg cggtcatcct gaccgggtgc 840
aaggccgaac acttcatcgt cgagagcaac ggcggtggcg gcggccggag caagaggtgc 900
gtcggcctgg tggcgacgtg catgagcaac ggcatcacca agaagctccg cgtcgaggcg 960
aaggtgtcca tctcggcgag cggcgcgctc atgacgccgc cgctgctgcg caacagcggg 1020
ctcaagaacc gccacatcgg ccggaacctg cacctccacc cggtgtccat ggcgtggggc 1080
tacttcccgg acaacacgcc ggagccgcac atcccgggga agtgctacga gggcggcatc 1140
atcaccagca tgcaccgcgt cacggagcgc accatcatcg agacgccagc gctcggcccg 1200
ggcgccttcg ccgccctggt gccctgggag tccggccgcg acatgaagga gcggatgcgc 1260
cggtacgcgc gcacggcgca cgcgttcgcg ctggtgcgcg accgcggcgc cgggtccgtc 1320
gacggcgagg gccgcgtccg ctacgccccg agccgcgacg acgccgagga gctccgcgcc 1380
ggcctccgcc gcgcgctgcg catcctggtg gccgccggcg ccgccgaggt gggcacgcac 1440
cgcagcgacg gggcccgcct ccgatgcaag ggcgcgcgcg acgcggacgt ggaggcgttc 1500
ctcgacgagg tgaccgtgga gaaggggccg atgcactcga cgacggacaa gtggtcggtg 1560
ctctgctcgg cgcaccagat ggggagctgc cggatgggcg cgagcccccg cgacggcgcc 1620
gtcgacgtcg ccggcgagag ctgggaggcg gaggggctct acgtctgcga cggcagcctg 1680
ctcccgacgg cggtgggcgt gaacccgatg atcaccatac agtccatcgc ctactgcgtc 1740
gccaagggca tagccgactc gatggcacac ggcaaggagc agcgctag 1788
<210> 102
<211> 595
<212> PRT
<213> Oryza sativa
<400> 102
Met Val Glu Ser Ala Ser Met Val Asn Glu Asn Ser Glu Asn Pro Tyr
1 5 10 15
Trp Lys Ala Ile Gly Tyr Arg Val Glu Glu Pro Arg Arg Asp Arg Ala
20 25 30
Glu Ser Met Pro Ser Pro Ser Pro Ser Pro Val Ser Arg Arg Pro Leu
35 40 45
Asp Asn Gly Val Val Glu Thr Arg Ala Leu Thr Asp Thr Thr Leu Leu
50 55 60
Arg Ser Leu Ala Ala Lys Gly Leu Ala Val Arg Pro Gly Ala Ser Asp
65 70 75 80
Glu His His Thr Val Arg Cys Asp Ala Val Ile Val Gly Ser Gly Cys
85 90 95
Gly Gly Gly Val Ala Ala Ala Val Leu Ala Ser Ala Gly Tyr Lys Val
100 105 110
Val Val Val Glu Lys Gly Asp Tyr Phe Thr Lys Glu Asp Tyr Ser Ser
115 120 125
Ile Glu Gly Pro Ser Met Glu Arg Leu Phe Glu Arg Gly Gly Val Phe
130 135 140
Cys Thr Ser Asn Val Thr Thr Met Ile Phe Thr Gly Ala Thr Val Gly
145 150 155 160
Gly Gly Ser Ala Val Asn Trp Ser Ala Ser Ile Arg Thr Pro Ala Gly
165 170 175
Val Met Gln Glu Trp Ser Arg Glu His Gly Leu Ala Val Phe Ala Ser
180 185 190
Pro Gly Tyr Ala Arg Ala Met Asp Ala Val Cys Glu Arg Leu Gly Val
195 200 205
Thr Asp Ala Cys Arg Glu Glu Gly Phe Gln Asn Lys Val Val Arg Arg
210 215 220
Gly Cys Asp Ala Leu Gly Leu Arg Ala Asp Ala Val Pro Arg Asn Ser
225 230 235 240
Ser Glu Gly His Phe Cys Gly Ser Cys Asn Phe Gly Cys Pro Thr Gly
245 250 255
Asp Lys Lys Gly Thr Asp Thr Thr Trp Leu Val Asp Ala Val Glu Arg
260 265 270
Gly Ala Val Ile Leu Thr Gly Cys Lys Ala Glu His Phe Ile Val Glu
275 280 285
Ser Asn Gly Gly Gly Gly Gly Arg Ser Lys Arg Cys Val Gly Leu Val
290 295 300
Ala Thr Cys Met Ser Asn Gly Ile Thr Lys Lys Leu Arg Val Glu Ala
305 310 315 320
Lys Val Ser Ile Ser Ala Ser Gly Ala Leu Met Thr Pro Pro Leu Leu
325 330 335
Arg Asn Ser Gly Leu Lys Asn Arg His Ile Gly Arg Asn Leu His Leu
340 345 350
His Pro Val Ser Met Ala Trp Gly Tyr Phe Pro Asp Asn Thr Pro Glu
355 360 365
Pro His Ile Pro Gly Lys Cys Tyr Glu Gly Gly Ile Ile Thr Ser Met
370 375 380
His Arg Val Thr Glu Arg Thr Ile Ile Glu Thr Pro Ala Leu Gly Pro
385 390 395 400
Gly Ala Phe Ala Ala Leu Val Pro Trp Glu Ser Gly Arg Asp Met Lys
405 410 415
Glu Arg Met Arg Arg Tyr Ala Arg Thr Ala His Ala Phe Ala Leu Val
420 425 430
Arg Asp Arg Gly Ala Gly Ser Val Asp Gly Glu Gly Arg Val Arg Tyr
435 440 445
Ala Pro Ser Arg Asp Asp Ala Glu Glu Leu Arg Ala Gly Leu Arg Arg
450 455 460
Ala Leu Arg Ile Leu Val Ala Ala Gly Ala Ala Glu Val Gly Thr His
465 470 475 480
Arg Ser Asp Gly Ala Arg Leu Arg Cys Lys Gly Ala Arg Asp Ala Asp
485 490 495
Val Glu Ala Phe Leu Asp Glu Val Thr Val Glu Lys Gly Pro Met His
500 505 510
Ser Thr Thr Asp Lys Trp Ser Val Leu Cys Ser Ala His Gln Met Gly
515 520 525
Ser Cys Arg Met Gly Ala Ser Pro Arg Asp Gly Ala Val Asp Val Ala
530 535 540
Gly Glu Ser Trp Glu Ala Glu Gly Leu Tyr Val Cys Asp Gly Ser Leu
545 550 555 560
Leu Pro Thr Ala Val Gly Val Asn Pro Met Ile Thr Ile Gln Ser Ile
565 570 575
Ala Tyr Cys Val Ala Lys Gly Ile Ala Asp Ser Met Ala His Gly Lys
580 585 590
Glu Gln Arg
595
<210> 103
<211> 2307
<212> DNA
<213> Zea mays
<400> 103
atgggcgcgg gcaacaacaa gcggggacac cctctcctga ggggtgggag cgcgaagagg 60
gagcggtaca cgcacggctt ctcggcgtcc cagatggccg cgctcaccgc cctctgcggc 120
gcgctggtgc cgtccctgcc gccggcggac cgccggaacg gtcaccacca caacccgcag 180
cctgaggagg acggcggcag cagcaaggtc gtggaggagt tcctcctcgc gtcggccgcc 240
gacccgcccg tgcccggcga ggtggccgag ctgatgtcgc gcaagtgcct ccccgaggcg 300
ctggctctgg tgcgcgcggt gctgtggctg ctgggcacgc ggctcggctc gctggcgctg 360
tgcggggcgc ggtgcctgtc gtggcggttc ccgttcgtgc gccggttcga cgagctgccc 420
ctggagcacc gggaggccgc gctccggcgg tggagccggc tgaccctgct cccgccgctg 480
cgcatgttct tcctcatcac caaggtcttc tgcctctacg ttttctactc ctggaccgac 540
gagaactcaa agaatccgca ctggcgcgcg atcgggtaca gcccgccccc ggcggacgac 600
gagccggcgc cggcggaatc agaacgtccc gagaagcgtc ccctggacga cggcatcgtt 660
gagacgaaca aggagaacga cgcgtccctg cccgcgttgc tagccgagaa gggcctcagg 720
gtggccgatg acgcggcgcg gaacgtgtgc agggtcgagt gcgacgtcgt catcgtcggg 780
tccggctgcg gcgggggcgt ggcggcagcg gtgctcgcgg cggcggggca caaggtggtc 840
gtcatcgaga aggggaacta cttcacggcg cgggattaca cggccatcga ggcgccgtcg 900
atggagcacc tctacgaggg cggagggttc gtcagcacgc tcagcgccag cgcgctgctc 960
ctggcaggct cgacggtcgg cggcggcacg gcggtcaact ggtcggcctg catcaagacc 1020
cccgacgacg ttcgcgggga gtgggcgcgc gaccgggggc tcccgctgtt cgccaccggc 1080
gagtacgccg ccgccatgga caaggtgttc gagcggctcg gcgtgacggc cggttgtgcg 1140
gaggaggggc tccagaacaa ggtcctccgc aaggggtgcg agaggctcgg gtacaaggtg 1200
gagtcggtgt cgaggaactc gtcggagggg cactactgcg gcagctgcgg ctacggctgc 1260
cgcaccggcg acaagcgcgg cacggacagc acgtggctcg tcgacgcggt gagccgcggc 1320
gcggtgatcc tgacggggtg caaggccgag aagctgctgc tggagcctgg cagtgccgcg 1380
gacggcaggg cgaagcgatg cgtcggcgtg gtggcgagga gcaccaaccc ggcgatcacg 1440
aggacgctgg aggtgaccgc cagggtgaca gtctcggcgt gcgggtctct cctgacgccg 1500
gtgctgctgc gcgggagcgg gctccgcaac cggcatatcg ggaagaacct ccacctccac 1560
ccgacggccc tggtgtgggg ctacttcccg gacaccgtgc cggacctcag gggcaggatg 1620
tacgagggcg gcatcatcac gtccctgcac aaggtggaag gcggcggtcc gggcgcgccg 1680
gcccgcgcca tcctcgaggc gccggcgatg ggcctggccg gcgcggggac gcagttcccc 1740
tgggtgtccg ggcgcgacat gaaggagcgg atgctcaggt acgggcgcac ggtgcacctc 1800
ttctccatgg tgagggaccg cgggtccggc accgtgcacg gcgagcggcg ggtggcgtac 1860
cacctggacg ccacggaccg ggagaacatg cgcgacggca tgcggcgcgc gctgcgcgtc 1920
ctggccgcgg cgggcgcggc ggagatcggc acccaccgca gcgacgggca gcggttcgcg 1980
tgcgggggcg ccacggaggc cgcgctggag gagttcctgg acggcgtgga cgtggtgcgc 2040
gggccccagt cgaaggccga ggcgtggacc ctgtgctgca ccgcgcacca gatgggcagc 2100
tgccggatgg gcgccacggc ccgggacggc gccgtggacg cgcgcggcga gagctgggag 2160
gccgggagcc tgtacgtgtg cgacggcagc gtcctgcccg gcgccgtggg cgtcaaccct 2220
atggtcacca tccagtccgt ggcctactgc ctcgccacgg gcatcgccga gtcgctcagg 2280
cgcggcccgg tttcacgaaa ggattga 2307
<210> 104
<211> 768
<212> PRT
<213> Zea mays
<400> 104
Met Gly Ala Gly Asn Asn Lys Arg Gly His Pro Leu Leu Arg Gly Gly
1 5 10 15
Ser Ala Lys Arg Glu Arg Tyr Thr His Gly Phe Ser Ala Ser Gln Met
20 25 30
Ala Ala Leu Thr Ala Leu Cys Gly Ala Leu Val Pro Ser Leu Pro Pro
35 40 45
Ala Asp Arg Arg Asn Gly His His His Asn Pro Gln Pro Glu Glu Asp
50 55 60
Gly Gly Ser Ser Lys Val Val Glu Glu Phe Leu Leu Ala Ser Ala Ala
65 70 75 80
Asp Pro Pro Val Pro Gly Glu Val Ala Glu Leu Met Ser Arg Lys Cys
85 90 95
Leu Pro Glu Ala Leu Ala Leu Val Arg Ala Val Leu Trp Leu Leu Gly
100 105 110
Thr Arg Leu Gly Ser Leu Ala Leu Cys Gly Ala Arg Cys Leu Ser Trp
115 120 125
Arg Phe Pro Phe Val Arg Arg Phe Asp Glu Leu Pro Leu Glu His Arg
130 135 140
Glu Ala Ala Leu Arg Arg Trp Ser Arg Leu Thr Leu Leu Pro Pro Leu
145 150 155 160
Arg Met Phe Phe Leu Ile Thr Lys Val Phe Cys Leu Tyr Val Phe Tyr
165 170 175
Ser Trp Thr Asp Glu Asn Ser Lys Asn Pro His Trp Arg Ala Ile Gly
180 185 190
Tyr Ser Pro Pro Pro Ala Asp Asp Glu Pro Ala Pro Ala Glu Ser Glu
195 200 205
Arg Pro Glu Lys Arg Pro Leu Asp Asp Gly Ile Val Glu Thr Asn Lys
210 215 220
Glu Asn Asp Ala Ser Leu Pro Ala Leu Leu Ala Glu Lys Gly Leu Arg
225 230 235 240
Val Ala Asp Asp Ala Ala Arg Asn Val Cys Arg Val Glu Cys Asp Val
245 250 255
Val Ile Val Gly Ser Gly Cys Gly Gly Gly Val Ala Ala Ala Val Leu
260 265 270
Ala Ala Ala Gly His Lys Val Val Val Ile Glu Lys Gly Asn Tyr Phe
275 280 285
Thr Ala Arg Asp Tyr Thr Ala Ile Glu Ala Pro Ser Met Glu His Leu
290 295 300
Tyr Glu Gly Gly Gly Phe Val Ser Thr Leu Ser Ala Ser Ala Leu Leu
305 310 315 320
Leu Ala Gly Ser Thr Val Gly Gly Gly Thr Ala Val Asn Trp Ser Ala
325 330 335
Cys Ile Lys Thr Pro Asp Asp Val Arg Gly Glu Trp Ala Arg Asp Arg
340 345 350
Gly Leu Pro Leu Phe Ala Thr Gly Glu Tyr Ala Ala Ala Met Asp Lys
355 360 365
Val Phe Glu Arg Leu Gly Val Thr Ala Gly Cys Ala Glu Glu Gly Leu
370 375 380
Gln Asn Lys Val Leu Arg Lys Gly Cys Glu Arg Leu Gly Tyr Lys Val
385 390 395 400
Glu Ser Val Ser Arg Asn Ser Ser Glu Gly His Tyr Cys Gly Ser Cys
405 410 415
Gly Tyr Gly Cys Arg Thr Gly Asp Lys Arg Gly Thr Asp Ser Thr Trp
420 425 430
Leu Val Asp Ala Val Ser Arg Gly Ala Val Ile Leu Thr Gly Cys Lys
435 440 445
Ala Glu Lys Leu Leu Leu Glu Pro Gly Ser Ala Ala Asp Gly Arg Ala
450 455 460
Lys Arg Cys Val Gly Val Val Ala Arg Ser Thr Asn Pro Ala Ile Thr
465 470 475 480
Arg Thr Leu Glu Val Thr Ala Arg Val Thr Val Ser Ala Cys Gly Ser
485 490 495
Leu Leu Thr Pro Val Leu Leu Arg Gly Ser Gly Leu Arg Asn Arg His
500 505 510
Ile Gly Lys Asn Leu His Leu His Pro Thr Ala Leu Val Trp Gly Tyr
515 520 525
Phe Pro Asp Thr Val Pro Asp Leu Arg Gly Arg Met Tyr Glu Gly Gly
530 535 540
Ile Ile Thr Ser Leu His Lys Val Glu Gly Gly Gly Pro Gly Ala Pro
545 550 555 560
Ala Arg Ala Ile Leu Glu Ala Pro Ala Met Gly Leu Ala Gly Ala Gly
565 570 575
Thr Gln Phe Pro Trp Val Ser Gly Arg Asp Met Lys Glu Arg Met Leu
580 585 590
Arg Tyr Gly Arg Thr Val His Leu Phe Ser Met Val Arg Asp Arg Gly
595 600 605
Ser Gly Thr Val His Gly Glu Arg Arg Val Ala Tyr His Leu Asp Ala
610 615 620
Thr Asp Arg Glu Asn Met Arg Asp Gly Met Arg Arg Ala Leu Arg Val
625 630 635 640
Leu Ala Ala Ala Gly Ala Ala Glu Ile Gly Thr His Arg Ser Asp Gly
645 650 655
Gln Arg Phe Ala Cys Gly Gly Ala Thr Glu Ala Ala Leu Glu Glu Phe
660 665 670
Leu Asp Gly Val Asp Val Val Arg Gly Pro Gln Ser Lys Ala Glu Ala
675 680 685
Trp Thr Leu Cys Cys Thr Ala His Gln Met Gly Ser Cys Arg Met Gly
690 695 700
Ala Thr Ala Arg Asp Gly Ala Val Asp Ala Arg Gly Glu Ser Trp Glu
705 710 715 720
Ala Gly Ser Leu Tyr Val Cys Asp Gly Ser Val Leu Pro Gly Ala Val
725 730 735
Gly Val Asn Pro Met Val Thr Ile Gln Ser Val Ala Tyr Cys Leu Ala
740 745 750
Thr Gly Ile Ala Glu Ser Leu Arg Arg Gly Pro Val Ser Arg Lys Asp
755 760 765
<210> 105
<211> 2322
<212> DNA
<213> Sorghum bicolor
<400> 105
atgggcgcgg gcgacaagcg gggacaccct ctcctgaggg gaggagccgc gaacagggag 60
cggtacaccc acggcttctc ggcgtcccag atgaccgcgc tcaccgccct ctgcggcgcg 120
ttggtgccgt ccctgccgcc ggaccgccgg aacggtcacc agcagcagga ggacggcggc 180
cgcggcgggg gcagcggcgg caacaaggac aaggtcgtgg aggagttcct cctcgcctcg 240
gccgccgacc cgcccgtgcc cggcgaggtg gccgagcaga tgtcgcgcaa gtgcctcccc 300
gaggcgctgg cgctggtacg cacggtgctg tggctgctgg gcacccggct cggctcgctg 360
gcgctgtgcg gggcccggtc gtgcctgtcg tggcggttcc cgttcgtgcg ccggttcgac 420
gagctgcccc tggagcagcg ggaggccgcg ctccggcggt ggagccggca gaccctgctc 480
ctgccgctgc gcatgttctt cgtcattatc aaggccatct gcctccacgt cttctactcc 540
tggaccgatg agaactcaaa gaatccgcac tggcgggcga tcgggtacag cccgccgctg 600
gccgacgacg agccggcgcc ggcggaatca gagcgtcccg agaagcgtcc cctcgacgac 660
ggcgtcgtgg agacgaccaa ggagaccgac gcgtccctgc cggcgttgct agcggcgaag 720
ggcctcacgg tggccgatga ctcggcgcag aacgtgtgca gggtcgagtg cgacgtcgtc 780
atcgtcggtt ccgggtgcgg cgggggcgtg gcggcagcgg tgctcgcggg ggcggggcac 840
aaggtggtcg tcatcgagaa aggaaactac ttcacggcgc gggattacac ggctatcgag 900
gcgccgtcga tggagcagct ctacgagggc ggaggtgccg tcagcacgct cagcggcagc 960
gcgctagtcc tggcaggctc tacggttggc ggcggtacgg cggtgaactg gtcagcttgc 1020
atcaagaccc ctgacgacgt tcgcgaggag tgggcgagcg accaggggct cccgttgttc 1080
gccaccgacg agtacgccgc cgccatggac aaggtgttcg agcggctcgg cgtgacggcc 1140
gggtgcgcgg aggaggggct ccagaacaag gtcctccgca aggggtgcga gaagctcggg 1200
tacaaggtgg agtcggtgtc aaggaactcg tcggaggggc actactgcgg cagctgcggt 1260
tatggctgcc gcagcggcga caagcgcggc acggaccgaa cgtggctggt cgacgcggtg 1320
agccgcggcg cggtgatcct tacgggatgc aaggccgaga agctgctgct tgagcgtact 1380
ggcactggcg gcgcggagag cagggcgaag cgatgcgtcg gcgtggtggc gaggagcacc 1440
aacccgacga tcacgaggac gctggaggtg cgcgccaggg tgaccgtctc ggcgtgcggg 1500
tcgctcctga cgccggtgct gctgcgcggg agcgggctca gcaaccggca catcggcaag 1560
aacctccacc tccacccgac ggccctggtg tggggctact tcccggacac catgccggac 1620
ctcaagggca agacgtacga gggcggcatc atcacgtccc tgcacaaggt ggaaggcgct 1680
ccgggcgcgc cggcccgcgc catcctcgaa acgccggcga tgggcctggc cggcgcgggg 1740
acgctgttcc cttgggtgtc cgggagcgat ttgaaggagc ggatgctcag gtacggccgc 1800
acggtgcacc tcttctccct ggtgagggac cgcggttccg gcagcgtgct cggagagcgg 1860
cgggtcgcgt accacttgga cgccacggac gcggagaaca tgcgcgaggg cctgcgccgc 1920
gcgctgcgca tcctcgccgc ggcgggcgcg gcggagatcg gcacccaccg cagcgacggg 1980
cagcggttcg cgtgcagggg cgccacggag gcggcgctgg aggagttcct ggacggcgtg 2040
gacgtggtgc gcgggcccca gtcgaaggcc gaggcgtgga gcctgtgctg cacggcgcac 2100
cagatgggca gctgccggat gggcgtcacg gcccgggacg gcgccgtgga cgcgcgcggc 2160
gagagctggg aggtcgagag cctgtacgtg tgcgacggca gcgtcctacc cagcgccgtg 2220
ggtgtcaacc ccatggtcac catccagtcc gtggcctact gcctcgccac gggcatcgcc 2280
gagtcgctca ggcgtggccc ggttcccgaa aagatccagt ga 2322
<210> 106
<211> 773
<212> PRT
<213> Sorghum bicolor
<400> 106
Met Gly Ala Gly Asp Lys Arg Gly His Pro Leu Leu Arg Gly Gly Ala
1 5 10 15
Ala Asn Arg Glu Arg Tyr Thr His Gly Phe Ser Ala Ser Gln Met Thr
20 25 30
Ala Leu Thr Ala Leu Cys Gly Ala Leu Val Pro Ser Leu Pro Pro Asp
35 40 45
Arg Arg Asn Gly His Gln Gln Gln Glu Asp Gly Gly Arg Gly Gly Gly
50 55 60
Ser Gly Gly Asn Lys Asp Lys Val Val Glu Glu Phe Leu Leu Ala Ser
65 70 75 80
Ala Ala Asp Pro Pro Val Pro Gly Glu Val Ala Glu Gln Met Ser Arg
85 90 95
Lys Cys Leu Pro Glu Ala Leu Ala Leu Val Arg Thr Val Leu Trp Leu
100 105 110
Leu Gly Thr Arg Leu Gly Ser Leu Ala Leu Cys Gly Ala Arg Ser Cys
115 120 125
Leu Ser Trp Arg Phe Pro Phe Val Arg Arg Phe Asp Glu Leu Pro Leu
130 135 140
Glu Gln Arg Glu Ala Ala Leu Arg Arg Trp Ser Arg Gln Thr Leu Leu
145 150 155 160
Leu Pro Leu Arg Met Phe Phe Val Ile Ile Lys Ala Ile Cys Leu His
165 170 175
Val Phe Tyr Ser Trp Thr Asp Glu Asn Ser Lys Asn Pro His Trp Arg
180 185 190
Ala Ile Gly Tyr Ser Pro Pro Leu Ala Asp Asp Glu Pro Ala Pro Ala
195 200 205
Glu Ser Glu Arg Pro Glu Lys Arg Pro Leu Asp Asp Gly Val Val Glu
210 215 220
Thr Thr Lys Glu Thr Asp Ala Ser Leu Pro Ala Leu Leu Ala Ala Lys
225 230 235 240
Gly Leu Thr Val Ala Asp Asp Ser Ala Gln Asn Val Cys Arg Val Glu
245 250 255
Cys Asp Val Val Ile Val Gly Ser Gly Cys Gly Gly Gly Val Ala Ala
260 265 270
Ala Val Leu Ala Gly Ala Gly His Lys Val Val Val Ile Glu Lys Gly
275 280 285
Asn Tyr Phe Thr Ala Arg Asp Tyr Thr Ala Ile Glu Ala Pro Ser Met
290 295 300
Glu Gln Leu Tyr Glu Gly Gly Gly Ala Val Ser Thr Leu Ser Gly Ser
305 310 315 320
Ala Leu Val Leu Ala Gly Ser Thr Val Gly Gly Gly Thr Ala Val Asn
325 330 335
Trp Ser Ala Cys Ile Lys Thr Pro Asp Asp Val Arg Glu Glu Trp Ala
340 345 350
Ser Asp Gln Gly Leu Pro Leu Phe Ala Thr Asp Glu Tyr Ala Ala Ala
355 360 365
Met Asp Lys Val Phe Glu Arg Leu Gly Val Thr Ala Gly Cys Ala Glu
370 375 380
Glu Gly Leu Gln Asn Lys Val Leu Arg Lys Gly Cys Glu Lys Leu Gly
385 390 395 400
Tyr Lys Val Glu Ser Val Ser Arg Asn Ser Ser Glu Gly His Tyr Cys
405 410 415
Gly Ser Cys Gly Tyr Gly Cys Arg Ser Gly Asp Lys Arg Gly Thr Asp
420 425 430
Arg Thr Trp Leu Val Asp Ala Val Ser Arg Gly Ala Val Ile Leu Thr
435 440 445
Gly Cys Lys Ala Glu Lys Leu Leu Leu Glu Arg Thr Gly Thr Gly Gly
450 455 460
Ala Glu Ser Arg Ala Lys Arg Cys Val Gly Val Val Ala Arg Ser Thr
465 470 475 480
Asn Pro Thr Ile Thr Arg Thr Leu Glu Val Arg Ala Arg Val Thr Val
485 490 495
Ser Ala Cys Gly Ser Leu Leu Thr Pro Val Leu Leu Arg Gly Ser Gly
500 505 510
Leu Ser Asn Arg His Ile Gly Lys Asn Leu His Leu His Pro Thr Ala
515 520 525
Leu Val Trp Gly Tyr Phe Pro Asp Thr Met Pro Asp Leu Lys Gly Lys
530 535 540
Thr Tyr Glu Gly Gly Ile Ile Thr Ser Leu His Lys Val Glu Gly Ala
545 550 555 560
Pro Gly Ala Pro Ala Arg Ala Ile Leu Glu Thr Pro Ala Met Gly Leu
565 570 575
Ala Gly Ala Gly Thr Leu Phe Pro Trp Val Ser Gly Ser Asp Leu Lys
580 585 590
Glu Arg Met Leu Arg Tyr Gly Arg Thr Val His Leu Phe Ser Leu Val
595 600 605
Arg Asp Arg Gly Ser Gly Ser Val Leu Gly Glu Arg Arg Val Ala Tyr
610 615 620
His Leu Asp Ala Thr Asp Ala Glu Asn Met Arg Glu Gly Leu Arg Arg
625 630 635 640
Ala Leu Arg Ile Leu Ala Ala Ala Gly Ala Ala Glu Ile Gly Thr His
645 650 655
Arg Ser Asp Gly Gln Arg Phe Ala Cys Arg Gly Ala Thr Glu Ala Ala
660 665 670
Leu Glu Glu Phe Leu Asp Gly Val Asp Val Val Arg Gly Pro Gln Ser
675 680 685
Lys Ala Glu Ala Trp Ser Leu Cys Cys Thr Ala His Gln Met Gly Ser
690 695 700
Cys Arg Met Gly Val Thr Ala Arg Asp Gly Ala Val Asp Ala Arg Gly
705 710 715 720
Glu Ser Trp Glu Val Glu Ser Leu Tyr Val Cys Asp Gly Ser Val Leu
725 730 735
Pro Ser Ala Val Gly Val Asn Pro Met Val Thr Ile Gln Ser Val Ala
740 745 750
Tyr Cys Leu Ala Thr Gly Ile Ala Glu Ser Leu Arg Arg Gly Pro Val
755 760 765
Pro Glu Lys Ile Gln
770
<210> 107
<211> 2277
<212> DNA
<213> Arabidopsis thaliana
<400> 107
atggtaggag gaagaagatt agggaagaga ggaagccctt tgttgagatg gagtgtgaaa 60
caagaaagct tcagccatgg attctcaaaa tctgacctcc aagctctctc ttccatttgc 120
gacgctatta tgcctcctgt tccattagag agcttaaatc ttgagatgaa gttgaaggtt 180
ttgcgcaacg atgcactctt atctttcttc aagtcttctt cttctgagtc tcatgttcga 240
ccagatgagg tggcagagct actggcgacc aaggcaatac cattgacagt tttagtggtg 300
agaatagttt tgagaatact cacattcaga ctggggacgt tactgctttg cgggttagtc 360
tgtcttgaca agaagcattg gccttttctt ctcaagttct ctgaaatgtc cctggagaag 420
agggaaaagg ttcttcagag atggaacaca cagtggtata acccactagc gagaatcggc 480
tttatgatga tcaaagccat cttcttgttc tactacttca catggacaaa tgaaaattca 540
gaaaacccag tatgggatgc aattaattat agcgtggaaa ttggtgaaaa tgaggacatg 600
gaacaaaagg aaagacctct agatgaaggg atcatcgaga ctgcaaaaga agatgagatg 660
accatcaagc aacgtatgat caacaaaggc ctcaaagtca cagaagacag agaaagggac 720
acttacaaga tcgagtgtga tgcagtggta gtgggttctg gctgtggcgg aggggttgca 780
gctgcaattc tagcaaagtc gggccttagg gtggtagtta tcgagaaggg gaactacttt 840
gcccccagag actactcagc tctcgagggc ccttccatgt ttgagctgtt tgagtccaat 900
agcttgatga tgactcatga tggcaggttc cggttcatgg caggatcaac agtcggtggt 960
ggctctgtgg taaactgggc agcgtcctta aaaacacctg atgctatcat cgaagaatgg 1020
tcagtacatc gagggatttc aatatattct agtgagaaat ataaggcggc gatgggtatt 1080
gtctgcaaga ggttaggtgt cactgagaaa atcatcagag aagggtttca gaaccagatt 1140
ctgcggaaag gttgcgagaa gcttggtttg gatgtgacaa tagtgccaag gaattcaaca 1200
gagaaacatt attgcggtag ttgctcgtat ggatgcccaa ccggagagaa gagagggaca 1260
gattcaacct ggctggttga tgcagttaac aacaatgctg tgatcctaac acagtgcaag 1320
gctgagaaac taatcttggc ggataacgat gctaacaaaa gagaggagag cggacgaaga 1380
aaaagatgtt tgggagtcgc agcctctcta tcccaccaaa ccagaaagaa gcttcagatc 1440
aatgccaaag tgacaattgt ggcttgcggc tcgcttaaga caccaggatt gttggcttca 1500
agcgggttga agaattcaaa catcagtcgc ggtctccata tccaccctat catgatggct 1560
tggggctact tcccagaaaa gaattcagaa ttggaaggag cggctcatga gggagagatc 1620
gtcacttcat tgcattatgt gcatccaatg gactccacta cacctaacat cacactggag 1680
actcctgcta taggaccagg tacgttcgca gctctgaccc cgtgggtctc tgggtcagac 1740
atgaaagaaa gaatggccaa atacgcaaga acagctcata tttttgccat ggtgagagat 1800
gaaggagttg gagaggtgaa aggagacatt gtcaaataca gattaaccaa agcggacgaa 1860
gagaatctga caattgggtt aaagcaagca ctgaggatcc tagtagcagc aggagcagcg 1920
gaggtaggca catacaggag cgacgggcag agaatgaagt gtgatggaat caaacagaaa 1980
gatctagagg catttttaga taccgtaaac gcgccacctg gagttgtgtc catgagtaag 2040
cattggactc aatcctttac agcgcatcaa ataggatgtt gccgtatggg tgccacagaa 2100
aaggaaggag ccattgatgg aaaaggagag agctgggagg cagaagattt gtatgtctgt 2160
gatgcaagtg ttctgcctac agctcttggt gttaatccca tgatcaccgt tcagtccact 2220
gcttactgca tatccaacag aatagccgag ttaatgaaga agaggaagaa agactga 2277
<210> 108
<211> 758
<212> PRT
<213> Arabidopsis thaliana
<400> 108
Met Val Gly Gly Arg Arg Leu Gly Lys Arg Gly Ser Pro Leu Leu Arg
1 5 10 15
Trp Ser Val Lys Gln Glu Ser Phe Ser His Gly Phe Ser Lys Ser Asp
20 25 30
Leu Gln Ala Leu Ser Ser Ile Cys Asp Ala Ile Met Pro Pro Val Pro
35 40 45
Leu Glu Ser Leu Asn Leu Glu Met Lys Leu Lys Val Leu Arg Asn Asp
50 55 60
Ala Leu Leu Ser Phe Phe Lys Ser Ser Ser Ser Glu Ser His Val Arg
65 70 75 80
Pro Asp Glu Val Ala Glu Leu Leu Ala Thr Lys Ala Ile Pro Leu Thr
85 90 95
Val Leu Val Val Arg Ile Val Leu Arg Ile Leu Thr Phe Arg Leu Gly
100 105 110
Thr Leu Leu Leu Cys Gly Leu Val Cys Leu Asp Lys Lys His Trp Pro
115 120 125
Phe Leu Leu Lys Phe Ser Glu Met Ser Leu Glu Lys Arg Glu Lys Val
130 135 140
Leu Gln Arg Trp Asn Thr Gln Trp Tyr Asn Pro Leu Ala Arg Ile Gly
145 150 155 160
Phe Met Met Ile Lys Ala Ile Phe Leu Phe Tyr Tyr Phe Thr Trp Thr
165 170 175
Asn Glu Asn Ser Glu Asn Pro Val Trp Asp Ala Ile Asn Tyr Ser Val
180 185 190
Glu Ile Gly Glu Asn Glu Asp Met Glu Gln Lys Glu Arg Pro Leu Asp
195 200 205
Glu Gly Ile Ile Glu Thr Ala Lys Glu Asp Glu Met Thr Ile Lys Gln
210 215 220
Arg Met Ile Asn Lys Gly Leu Lys Val Thr Glu Asp Arg Glu Arg Asp
225 230 235 240
Thr Tyr Lys Ile Glu Cys Asp Ala Val Val Val Gly Ser Gly Cys Gly
245 250 255
Gly Gly Val Ala Ala Ala Ile Leu Ala Lys Ser Gly Leu Arg Val Val
260 265 270
Val Ile Glu Lys Gly Asn Tyr Phe Ala Pro Arg Asp Tyr Ser Ala Leu
275 280 285
Glu Gly Pro Ser Met Phe Glu Leu Phe Glu Ser Asn Ser Leu Met Met
290 295 300
Thr His Asp Gly Arg Phe Arg Phe Met Ala Gly Ser Thr Val Gly Gly
305 310 315 320
Gly Ser Val Val Asn Trp Ala Ala Ser Leu Lys Thr Pro Asp Ala Ile
325 330 335
Ile Glu Glu Trp Ser Val His Arg Gly Ile Ser Ile Tyr Ser Ser Glu
340 345 350
Lys Tyr Lys Ala Ala Met Gly Ile Val Cys Lys Arg Leu Gly Val Thr
355 360 365
Glu Lys Ile Ile Arg Glu Gly Phe Gln Asn Gln Ile Leu Arg Lys Gly
370 375 380
Cys Glu Lys Leu Gly Leu Asp Val Thr Ile Val Pro Arg Asn Ser Thr
385 390 395 400
Glu Lys His Tyr Cys Gly Ser Cys Ser Tyr Gly Cys Pro Thr Gly Glu
405 410 415
Lys Arg Gly Thr Asp Ser Thr Trp Leu Val Asp Ala Val Asn Asn Asn
420 425 430
Ala Val Ile Leu Thr Gln Cys Lys Ala Glu Lys Leu Ile Leu Ala Asp
435 440 445
Asn Asp Ala Asn Lys Arg Glu Glu Ser Gly Arg Arg Lys Arg Cys Leu
450 455 460
Gly Val Ala Ala Ser Leu Ser His Gln Thr Arg Lys Lys Leu Gln Ile
465 470 475 480
Asn Ala Lys Val Thr Ile Val Ala Cys Gly Ser Leu Lys Thr Pro Gly
485 490 495
Leu Leu Ala Ser Ser Gly Leu Lys Asn Ser Asn Ile Ser Arg Gly Leu
500 505 510
His Ile His Pro Ile Met Met Ala Trp Gly Tyr Phe Pro Glu Lys Asn
515 520 525
Ser Glu Leu Glu Gly Ala Ala His Glu Gly Glu Ile Val Thr Ser Leu
530 535 540
His Tyr Val His Pro Met Asp Ser Thr Thr Pro Asn Ile Thr Leu Glu
545 550 555 560
Thr Pro Ala Ile Gly Pro Gly Thr Phe Ala Ala Leu Thr Pro Trp Val
565 570 575
Ser Gly Ser Asp Met Lys Glu Arg Met Ala Lys Tyr Ala Arg Thr Ala
580 585 590
His Ile Phe Ala Met Val Arg Asp Glu Gly Val Gly Glu Val Lys Gly
595 600 605
Asp Ile Val Lys Tyr Arg Leu Thr Lys Ala Asp Glu Glu Asn Leu Thr
610 615 620
Ile Gly Leu Lys Gln Ala Leu Arg Ile Leu Val Ala Ala Gly Ala Ala
625 630 635 640
Glu Val Gly Thr Tyr Arg Ser Asp Gly Gln Arg Met Lys Cys Asp Gly
645 650 655
Ile Lys Gln Lys Asp Leu Glu Ala Phe Leu Asp Thr Val Asn Ala Pro
660 665 670
Pro Gly Val Val Ser Met Ser Lys His Trp Thr Gln Ser Phe Thr Ala
675 680 685
His Gln Ile Gly Cys Cys Arg Met Gly Ala Thr Glu Lys Glu Gly Ala
690 695 700
Ile Asp Gly Lys Gly Glu Ser Trp Glu Ala Glu Asp Leu Tyr Val Cys
705 710 715 720
Asp Ala Ser Val Leu Pro Thr Ala Leu Gly Val Asn Pro Met Ile Thr
725 730 735
Val Gln Ser Thr Ala Tyr Cys Ile Ser Asn Arg Ile Ala Glu Leu Met
740 745 750
Lys Lys Arg Lys Lys Asp
755
<210> 109
<211> 2256
<212> DNA
<213> Glycine max
<400> 109
atgaccagaa aagagtgtca tccattgtta aggggtggga gaggggatag caaatataaa 60
catggatttt ctgcagctga gatggagtca ctggcaagca tatgtgaggt tgtgttgcct 120
cctttgccta tggatgctct taagatcaga aaggaagacc aaattgatga ttatgattct 180
agcaagagtc tcaagtcctt ctgggacatt tctgcttctc gctatccaat ccctcatgag 240
gttgctgaga tgttaacgaa gaggagctta attgaagcag taatactgat tagagtagtt 300
ttatggctgc tggcaacaag gttgggaacc ttgttgctct gtggtttcct ctgtcttggt 360
gaaaaatggc cctatgtcaa caacttctca aacatatctt tggagaaaag agaaatggtt 420
atgcagaagt ggctgaaaca taggttcctc acacctatta gactggcctt tgcttacatc 480
aaagtcttgt gtctctttgt ttttttctct tgggttgatg aaaatggtga caacccagca 540
tggaaagcca ttggatatga ggtaccagct gatgaaaact tgaccaatgc ctccaaaact 600
aggccccttg aaaaggggat tatagaaacc atgaatgaat ctgactccgc tcttcaacaa 660
tctcttgcta acaaaggcct taatgttaca ctggactcaa aaagcaacat cctcaaagtc 720
aaatgtgatg cactagttgt tggttctggt tgtggaggag gtgttgcggc tgctgttctt 780
tcaagtgctg gctacaaggt ggttgttctt gagaaaggaa actatttttc tactcaggat 840
tattcatctc tagaaggtcc ttccatgaat caactatatg aaactggagg gatccttgct 900
tctgtggact caagagtgct agttttggca ggatcaacag tgggtggtgg ctctgctgtt 960
aattggtcag cctgcattaa gaccccacac aaggtgctaa atgagtggtc tgagaatcac 1020
aagcttccct tcttttcaag ccaagaatat ctctccgcaa tggaaactgt gtgtgaaagg 1080
attggtgtaa cagaaaactg tacacaagag ggattccaaa accaagtgct gagaaaaggg 1140
tgtcaaaatc ttggcctcaa agttgactat gtgccaagaa actcctcagg gaatcactac 1200
tgtggctcat gtggttatgg ctgtccgaaa ggagagaaac aagggactca agctacatgg 1260
cttgtagatg cagttgaaag agatgcagta ataataacag gatgcaaagc tgagaggttc 1320
ttgttggaaa gcaacaggag tggaaatggc agaaagaaga aatgtttggg agtgatggca 1380
aaagcattaa acagcagagt cacaatgaag ttacaaattg aggccaaggt gacaatttct 1440
gcaggtgggg cacttttgac accccctttg ttaatctcta gtggactaaa aaacaagaac 1500
ataggtaaaa accttcatct ccaccctgtg ctaatgacat ggggatactt tccagaatca 1560
aatgattcag aattcaaagg aaaagtctac gagggaggta taataacatc agtccacaaa 1620
gtgccatcaa cagactccaa ttcagattca agggcaataa ttgaaacccc ttcgctagga 1680
ccagcttcct ttgcagcact gtgtccttgg gagtcaggac tagactttaa ggaaagaatg 1740
ctgaattacc ctagaacttc acatttgata acaataataa gagacatggc ttgtggacag 1800
gtatcaacag aaggaaggat cagctacaag ctgaatgaaa ttgacaagga gaacatgaag 1860
gctggcttga aacaagcact gaagattctt atagctgcag gagcagttga ggtaggtaca 1920
cacagaagtg atggtcagag acttaagtgt gatggcattg gtgaaaatga agtgcaagag 1980
ttcttggaca gtgtgtgtcc aatggagggg gcactttcac caggtgagta ttggaacata 2040
tattcttctg cacatcaaat ggggagctgc agaatgggag tcaatgagaa agaaggtgca 2100
gttgatgaaa atggtgagac atgggaggct gaagggttgt ttgtttgtga tgctagtgtg 2160
cttccaagtg ctgttggtgt caatcccatg atcacggtcc aatcaactgc atactgcatc 2220
tctaatagaa tagcagatta tcttagaagg gattaa 2256
<210> 110
<211> 751
<212> PRT
<213> Glycine max
<400> 110
Met Thr Arg Lys Glu Cys His Pro Leu Leu Arg Gly Gly Arg Gly Asp
1 5 10 15
Ser Lys Tyr Lys His Gly Phe Ser Ala Ala Glu Met Glu Ser Leu Ala
20 25 30
Ser Ile Cys Glu Val Val Leu Pro Pro Leu Pro Met Asp Ala Leu Lys
35 40 45
Ile Arg Lys Glu Asp Gln Ile Asp Asp Tyr Asp Ser Ser Lys Ser Leu
50 55 60
Lys Ser Phe Trp Asp Ile Ser Ala Ser Arg Tyr Pro Ile Pro His Glu
65 70 75 80
Val Ala Glu Met Leu Thr Lys Arg Ser Leu Ile Glu Ala Val Ile Leu
85 90 95
Ile Arg Val Val Leu Trp Leu Leu Ala Thr Arg Leu Gly Thr Leu Leu
100 105 110
Leu Cys Gly Phe Leu Cys Leu Gly Glu Lys Trp Pro Tyr Val Asn Asn
115 120 125
Phe Ser Asn Ile Ser Leu Glu Lys Arg Glu Met Val Met Gln Lys Trp
130 135 140
Leu Lys His Arg Phe Leu Thr Pro Ile Arg Leu Ala Phe Ala Tyr Ile
145 150 155 160
Lys Val Leu Cys Leu Phe Val Phe Phe Ser Trp Val Asp Glu Asn Gly
165 170 175
Asp Asn Pro Ala Trp Lys Ala Ile Gly Tyr Glu Val Pro Ala Asp Glu
180 185 190
Asn Leu Thr Asn Ala Ser Lys Thr Arg Pro Leu Glu Lys Gly Ile Ile
195 200 205
Glu Thr Met Asn Glu Ser Asp Ser Ala Leu Gln Gln Ser Leu Ala Asn
210 215 220
Lys Gly Leu Asn Val Thr Leu Asp Ser Lys Ser Asn Ile Leu Lys Val
225 230 235 240
Lys Cys Asp Ala Leu Val Val Gly Ser Gly Cys Gly Gly Gly Val Ala
245 250 255
Ala Ala Val Leu Ser Ser Ala Gly Tyr Lys Val Val Val Leu Glu Lys
260 265 270
Gly Asn Tyr Phe Ser Thr Gln Asp Tyr Ser Ser Leu Glu Gly Pro Ser
275 280 285
Met Asn Gln Leu Tyr Glu Thr Gly Gly Ile Leu Ala Ser Val Asp Ser
290 295 300
Arg Val Leu Val Leu Ala Gly Ser Thr Val Gly Gly Gly Ser Ala Val
305 310 315 320
Asn Trp Ser Ala Cys Ile Lys Thr Pro His Lys Val Leu Asn Glu Trp
325 330 335
Ser Glu Asn His Lys Leu Pro Phe Phe Ser Ser Gln Glu Tyr Leu Ser
340 345 350
Ala Met Glu Thr Val Cys Glu Arg Ile Gly Val Thr Glu Asn Cys Thr
355 360 365
Gln Glu Gly Phe Gln Asn Gln Val Leu Arg Lys Gly Cys Gln Asn Leu
370 375 380
Gly Leu Lys Val Asp Tyr Val Pro Arg Asn Ser Ser Gly Asn His Tyr
385 390 395 400
Cys Gly Ser Cys Gly Tyr Gly Cys Pro Lys Gly Glu Lys Gln Gly Thr
405 410 415
Gln Ala Thr Trp Leu Val Asp Ala Val Glu Arg Asp Ala Val Ile Ile
420 425 430
Thr Gly Cys Lys Ala Glu Arg Phe Leu Leu Glu Ser Asn Arg Ser Gly
435 440 445
Asn Gly Arg Lys Lys Lys Cys Leu Gly Val Met Ala Lys Ala Leu Asn
450 455 460
Ser Arg Val Thr Met Lys Leu Gln Ile Glu Ala Lys Val Thr Ile Ser
465 470 475 480
Ala Gly Gly Ala Leu Leu Thr Pro Pro Leu Leu Ile Ser Ser Gly Leu
485 490 495
Lys Asn Lys Asn Ile Gly Lys Asn Leu His Leu His Pro Val Leu Met
500 505 510
Thr Trp Gly Tyr Phe Pro Glu Ser Asn Asp Ser Glu Phe Lys Gly Lys
515 520 525
Val Tyr Glu Gly Gly Ile Ile Thr Ser Val His Lys Val Pro Ser Thr
530 535 540
Asp Ser Asn Ser Asp Ser Arg Ala Ile Ile Glu Thr Pro Ser Leu Gly
545 550 555 560
Pro Ala Ser Phe Ala Ala Leu Cys Pro Trp Glu Ser Gly Leu Asp Phe
565 570 575
Lys Glu Arg Met Leu Asn Tyr Pro Arg Thr Ser His Leu Ile Thr Ile
580 585 590
Ile Arg Asp Met Ala Cys Gly Gln Val Ser Thr Glu Gly Arg Ile Ser
595 600 605
Tyr Lys Leu Asn Glu Ile Asp Lys Glu Asn Met Lys Ala Gly Leu Lys
610 615 620
Gln Ala Leu Lys Ile Leu Ile Ala Ala Gly Ala Val Glu Val Gly Thr
625 630 635 640
His Arg Ser Asp Gly Gln Arg Leu Lys Cys Asp Gly Ile Gly Glu Asn
645 650 655
Glu Val Gln Glu Phe Leu Asp Ser Val Cys Pro Met Glu Gly Ala Leu
660 665 670
Ser Pro Gly Glu Tyr Trp Asn Ile Tyr Ser Ser Ala His Gln Met Gly
675 680 685
Ser Cys Arg Met Gly Val Asn Glu Lys Glu Gly Ala Val Asp Glu Asn
690 695 700
Gly Glu Thr Trp Glu Ala Glu Gly Leu Phe Val Cys Asp Ala Ser Val
705 710 715 720
Leu Pro Ser Ala Val Gly Val Asn Pro Met Ile Thr Val Gln Ser Thr
725 730 735
Ala Tyr Cys Ile Ser Asn Arg Ile Ala Asp Tyr Leu Arg Arg Asp
740 745 750
<210> 111
<211> 315
<212> DNA
<213> Oryza sativa
<400> 111
atggccctcg cgtgtgcgtc tcatttgcgc cgcctcggag ccggagctcc ggcgagatcc 60
ttccacgcgc atccgtacca agccaaggtc ggcgtggtgg agttcctgaa cggggtcggg 120
aagggggtgg agacgcacgc cgccaaggtg gaggaggcgg tcggcggcga cctccagagc 180
ctcctccacg cccgcactct gcggctcaag aagctcggca tcccctgcaa gcagaggaaa 240
ttaattttga gtttcgctca caagtatcgt cttggtcttt ggaagcccca agcagaatcc 300
aagaaaacac agtga 315
<210> 112
<211> 104
<212> PRT
<213> Oryza sativa
<400> 112
Met Ala Leu Ala Cys Ala Ser His Leu Arg Arg Leu Gly Ala Gly Ala
1 5 10 15
Pro Ala Arg Ser Phe His Ala His Pro Tyr Gln Ala Lys Val Gly Val
20 25 30
Val Glu Phe Leu Asn Gly Val Gly Lys Gly Val Glu Thr His Ala Ala
35 40 45
Lys Val Glu Glu Ala Val Gly Gly Asp Leu Gln Ser Leu Leu His Ala
50 55 60
Arg Thr Leu Arg Leu Lys Lys Leu Gly Ile Pro Cys Lys Gln Arg Lys
65 70 75 80
Leu Ile Leu Ser Phe Ala His Lys Tyr Arg Leu Gly Leu Trp Lys Pro
85 90 95
Gln Ala Glu Ser Lys Lys Thr Gln
100
<210> 113
<211> 312
<212> DNA
<213> Zea mays
<400> 113
atggctctcg cgtgcgcctc tcacgcgtgc cgctttctcc tcgccggcca ggcgagatct 60
ttccacgccc agccctacca tgccaaggtc ggcgtggtgg agttcctgaa cggcgtcggg 120
aaaggggtgg aggcgcacgc cgcgaagctg gaggaggctg tgggcggcga cctccagagg 180
ctccttgagg cccgcacgct gcggctcaag aagctcggca tcccctgcaa gcatagaaaa 240
ttgattttga gttttgcaca caagtaccgt cttggtctct ggaagcccca agcagaaccc 300
aggaaagttt aa 312
<210> 114
<211> 103
<212> PRT
<213> Zea mays
<400> 114
Met Ala Leu Ala Cys Ala Ser His Ala Cys Arg Phe Leu Leu Ala Gly
1 5 10 15
Gln Ala Arg Ser Phe His Ala Gln Pro Tyr His Ala Lys Val Gly Val
20 25 30
Val Glu Phe Leu Asn Gly Val Gly Lys Gly Val Glu Ala His Ala Ala
35 40 45
Lys Leu Glu Glu Ala Val Gly Gly Asp Leu Gln Arg Leu Leu Glu Ala
50 55 60
Arg Thr Leu Arg Leu Lys Lys Leu Gly Ile Pro Cys Lys His Arg Lys
65 70 75 80
Leu Ile Leu Ser Phe Ala His Lys Tyr Arg Leu Gly Leu Trp Lys Pro
85 90 95
Gln Ala Glu Pro Arg Lys Val
100
<210> 115
<211> 315
<212> DNA
<213> Sorghum bicolor
<400> 115
atggctctcg cgtgcgccac tcacgcgcgc cgcttcctcc tcgccggtcc ggcgagatct 60
ttccacgccc agccctacca agccaaggtc ggcgtggtgg agttcctgaa cggcgtcggc 120
aagggggtgg aggcgcacgc cgcgaagctg gaggatgctg tgggcggcga cctccagagg 180
ctcctcgaga cccgcacgct gcggctcaag aagctcggca tcccctgcaa acatagaaag 240
ttgattttga gttttgctca taagtaccgt cttggtctct ggaagcccca ggcagatccc 300
aggaaagttg aataa 315
<210> 116
<211> 104
<212> PRT
<213> Sorghum bicolor
<400> 116
Met Ala Leu Ala Cys Ala Thr His Ala Arg Arg Phe Leu Leu Ala Gly
1 5 10 15
Pro Ala Arg Ser Phe His Ala Gln Pro Tyr Gln Ala Lys Val Gly Val
20 25 30
Val Glu Phe Leu Asn Gly Val Gly Lys Gly Val Glu Ala His Ala Ala
35 40 45
Lys Leu Glu Asp Ala Val Gly Gly Asp Leu Gln Arg Leu Leu Glu Thr
50 55 60
Arg Thr Leu Arg Leu Lys Lys Leu Gly Ile Pro Cys Lys His Arg Lys
65 70 75 80
Leu Ile Leu Ser Phe Ala His Lys Tyr Arg Leu Gly Leu Trp Lys Pro
85 90 95
Gln Ala Asp Pro Arg Lys Val Glu
100
<210> 117
<211> 339
<212> DNA
<213> Arabidopsis thaliana
<400> 117
atggcgtgga tgcgactgat acagagcgcg agatctgttc ttacgacagc accagcgtct 60
tcgactctta acttccatag attctactcc aaatccgccg ctccttatca cgtgaaagtt 120
ggaattcctg agtttttgag tgggattggt ggaggagttg agactcatat tgctaagctt 180
gaaaccgagt taggcgatct tccgaaattg ctcgtgactc gtacgcttag actcaagaag 240
tttggtattc cttgcaaaca taggaaattg atactgaaat atagccacaa atacaggcta 300
ggtctatgga aacctagagc tgacgcaata aaggcgtga 339
<210> 118
<211> 112
<212> PRT
<213> Arabidopsis thaliana
<400> 118
Met Ala Trp Met Arg Leu Ile Gln Ser Ala Arg Ser Val Leu Thr Thr
1 5 10 15
Ala Pro Ala Ser Ser Thr Leu Asn Phe His Arg Phe Tyr Ser Lys Ser
20 25 30
Ala Ala Pro Tyr His Val Lys Val Gly Ile Pro Glu Phe Leu Ser Gly
35 40 45
Ile Gly Gly Gly Val Glu Thr His Ile Ala Lys Leu Glu Thr Glu Leu
50 55 60
Gly Asp Leu Pro Lys Leu Leu Val Thr Arg Thr Leu Arg Leu Lys Lys
65 70 75 80
Phe Gly Ile Pro Cys Lys His Arg Lys Leu Ile Leu Lys Tyr Ser His
85 90 95
Lys Tyr Arg Leu Gly Leu Trp Lys Pro Arg Ala Asp Ala Ile Lys Ala
100 105 110
<210> 119
<211> 375
<212> DNA
<213> Glycine max
<400> 119
atggacggtg cgagtgggag aggcagcaag aagagaaaaa ggggaatggc atggttgcag 60
ttgcagagaa tcatcaccaa taccaaggga ggtgctaatt tgatctcacc gctcccacga 120
ttcttctcga aatgttcccc ctatgttttg aaagttggaa taccagagtt tctaaacggg 180
attgggaagg gggttgagtc ccacgttcct aagcttgaat ctgaaatcgg tgatttccag 240
aaacttctcg tcattcgaac ccttaaactc aagaagctcg gtattccttg caaacatagg 300
aagttaatat tgaaatacac acacaagtac aggctgggat tatggaggcc acgtgctgag 360
ttcatcactg cctga 375
<210> 120
<211> 124
<212> PRT
<213> Glycine max
<400> 120
Met Asp Gly Ala Ser Gly Arg Gly Ser Lys Lys Arg Lys Arg Gly Met
1 5 10 15
Ala Trp Leu Gln Leu Gln Arg Ile Ile Thr Asn Thr Lys Gly Gly Ala
20 25 30
Asn Leu Ile Ser Pro Leu Pro Arg Phe Phe Ser Lys Cys Ser Pro Tyr
35 40 45
Val Leu Lys Val Gly Ile Pro Glu Phe Leu Asn Gly Ile Gly Lys Gly
50 55 60
Val Glu Ser His Val Pro Lys Leu Glu Ser Glu Ile Gly Asp Phe Gln
65 70 75 80
Lys Leu Leu Val Ile Arg Thr Leu Lys Leu Lys Lys Leu Gly Ile Pro
85 90 95
Cys Lys His Arg Lys Leu Ile Leu Lys Tyr Thr His Lys Tyr Arg Leu
100 105 110
Gly Leu Trp Arg Pro Arg Ala Glu Phe Ile Thr Ala
115 120
<210> 121
<211> 1215
<212> DNA
<213> Oryza sativa
<400> 121
atggtggtgt cgcgggcgcg ggtgcaggac ccggcgaccg gcgagcaccg cgacgtgatg 60
tacaagggca tggcgtcgga gctgttcgtg ccgtacatgg acccgacgga ggcgtggtac 120
ttcaagacgt acatggacgc cggcgagtac ggcttcgggc tgcaggccat gccgctcgtc 180
ccgctcaacg actgcccgcg ccacgcccgc tacctcgacg ccgtcttcgt cgccgccgac 240
ggccgcccct acgtgcgcga gaacatgatc tgcgtcttcg agcgctacgc cggcgacatc 300
gcgtggcgac actccgagag ccccatcacc ggcatggaca taagggagtc gcggccgaag 360
gtgacgctgg tggcgcgcat ggcggcgtcg gtggccaact acgactatat cgtcgactgg 420
gagttccaga tggacggcct cgttcgcatc aaggttgggc taagtgggat cctcatggtg 480
aagggcaccc agtactccca catgaaccag gtccatcaaa atgacaatat gtacggcacc 540
cttctgtctg agaacgttat tggcgtcatc catgaccact ttgtcacttt ccggctcgac 600
atggacattg acggcgccga caactccttc gtcaaggtgg cgatggcacg acagaacacc 660
ggcgccggcg aatcccctcg taagagctac ctgaaggcta cccgacatgt cgcaaggacg 720
gagaaagatg cccaggtccg cctgaagcta tatgagccat ccgagttcca cattgtcaac 780
cccatgaaga agacacgagt tgggaaccct gttggttata aggttgtccc agccggtact 840
gcagctagcc tgttggatcc agaggaccca cctcagaaga ggggtgcatt cacaaataat 900
cagatttggg tgacacccta caacaaaaca gaggaatggg ctggtggtct atttgtctac 960
cagagcaaag gggaggacac acttgcaact tggtccgaaa gggaccgtcc gatcgagaac 1020
aaggacctgg tgctgtggta cacgctgggg tttcaccatg tcccgtgcca ggaggacttc 1080
cccatcatgc ccacggtgtc gtccagcttc gaccttaagc cagtgaactt ctttgagagc 1140
aaccccatcc tagggcagcg tccgacccag gagaacgacc tgccggtgtg tgccgccgct 1200
gccacgacct cttga 1215
<210> 122
<211> 404
<212> PRT
<213> Oryza sativa
<400> 122
Met Val Val Ser Arg Ala Arg Val Gln Asp Pro Ala Thr Gly Glu His
1 5 10 15
Arg Asp Val Met Tyr Lys Gly Met Ala Ser Glu Leu Phe Val Pro Tyr
20 25 30
Met Asp Pro Thr Glu Ala Trp Tyr Phe Lys Thr Tyr Met Asp Ala Gly
35 40 45
Glu Tyr Gly Phe Gly Leu Gln Ala Met Pro Leu Val Pro Leu Asn Asp
50 55 60
Cys Pro Arg His Ala Arg Tyr Leu Asp Ala Val Phe Val Ala Ala Asp
65 70 75 80
Gly Arg Pro Tyr Val Arg Glu Asn Met Ile Cys Val Phe Glu Arg Tyr
85 90 95
Ala Gly Asp Ile Ala Trp Arg His Ser Glu Ser Pro Ile Thr Gly Met
100 105 110
Asp Ile Arg Glu Ser Arg Pro Lys Val Thr Leu Val Ala Arg Met Ala
115 120 125
Ala Ser Val Ala Asn Tyr Asp Tyr Ile Val Asp Trp Glu Phe Gln Met
130 135 140
Asp Gly Leu Val Arg Ile Lys Val Gly Leu Ser Gly Ile Leu Met Val
145 150 155 160
Lys Gly Thr Gln Tyr Ser His Met Asn Gln Val His Gln Asn Asp Asn
165 170 175
Met Tyr Gly Thr Leu Leu Ser Glu Asn Val Ile Gly Val Ile His Asp
180 185 190
His Phe Val Thr Phe Arg Leu Asp Met Asp Ile Asp Gly Ala Asp Asn
195 200 205
Ser Phe Val Lys Val Ala Met Ala Arg Gln Asn Thr Gly Ala Gly Glu
210 215 220
Ser Pro Arg Lys Ser Tyr Leu Lys Ala Thr Arg His Val Ala Arg Thr
225 230 235 240
Glu Lys Asp Ala Gln Val Arg Leu Lys Leu Tyr Glu Pro Ser Glu Phe
245 250 255
His Ile Val Asn Pro Met Lys Lys Thr Arg Val Gly Asn Pro Val Gly
260 265 270
Tyr Lys Val Val Pro Ala Gly Thr Ala Ala Ser Leu Leu Asp Pro Glu
275 280 285
Asp Pro Pro Gln Lys Arg Gly Ala Phe Thr Asn Asn Gln Ile Trp Val
290 295 300
Thr Pro Tyr Asn Lys Thr Glu Glu Trp Ala Gly Gly Leu Phe Val Tyr
305 310 315 320
Gln Ser Lys Gly Glu Asp Thr Leu Ala Thr Trp Ser Glu Arg Asp Arg
325 330 335
Pro Ile Glu Asn Lys Asp Leu Val Leu Trp Tyr Thr Leu Gly Phe His
340 345 350
His Val Pro Cys Gln Glu Asp Phe Pro Ile Met Pro Thr Val Ser Ser
355 360 365
Ser Phe Asp Leu Lys Pro Val Asn Phe Phe Glu Ser Asn Pro Ile Leu
370 375 380
Gly Gln Arg Pro Thr Gln Glu Asn Asp Leu Pro Val Cys Ala Ala Ala
385 390 395 400
Ala Thr Thr Ser
<210> 123
<211> 2235
<212> DNA
<213> Zea mays
<400> 123
atggatcgct ccacctccct gctccggctc atcttgcttg ctcttggcgc agccctggtg 60
ctcctcgtcg tccgctccgc cttccgcctc ccacgtggaa tcgacacacc caccacctca 120
ctcttcgatg acgaagccac cgcgggcagc agctgcaccc ggttcgcgcc gtgggggtgc 180
cggcaagccg accggattaa gcagaagccg ccgtcgcacg agaacgacgt gccgcggcac 240
ccgctcgacc cactgacgat cagcgaggta aaccgtgcgc gcgagctcct ccgcgcgcac 300
ccgccgttcg catcgtcgcc gtcgtccatg ttcgtgcact cgctcgcgct cgacgagccg 360
gacaagcccg tcgtcctgag ctggcggaag ggcgtcgacc cgctgccccc gcggcgcgcg 420
gtggcggtgg tccggttccg cggtgaggcc ttcgtcctgg ccattgacct cgccagcggc 480
gccgtgactc ctctgcctgt cccagcttcc gggtacccga ccatgaccat ggacgagcag 540
gtgctcctct gctacacgcc tttcagggac ccggcgttca acgcgaccat ccagcggcac 600
ggcgtccgcc tgtccgatgt cgcctgcctg cccatctcgc tcgggtggta cggccccagc 660
gaggagaacc ggcggctgat caagatccag tgcttctccg cggagggcac cgccaacttc 720
tacatgcgcc ccatcgaggg cctcaccgtg ctggtggaca tggacacgag ggaggtggtc 780
cgcatctccg accgcggcgc cggcatcccc atcccgcccg ccgccaacac cgactaccgg 840
tactcccgcc acatgcaaga cgaaggcgac gaccagcaga cagcggggtt ccagaaggtg 900
cgggcgccgt cgatggaacc ggggccgtcg tcagggccgc gggtggagct ggtggacggc 960
cacacggtgc ggtggggcgg gtgggagttc cacctcaagg cggacgcgcg cgccggcatg 1020
gtggtgtcgc gcgcacgggt gcaggacccc ggcacgggcg cgcaccggga ggtgctgtac 1080
aagggcatgg cgtcggagct gttcgtaccg tacatggacc ccaccgaggc gtggtacttc 1140
aagacgtaca tggacgccgg cgagtacggc ttcggcctgc aggccatgcc gctggtgccg 1200
ctcaacgact gcccgcgcca cgcgcgctac ctggacggcg tgttcgtggc cgccgacgga 1260
cggccctacg tgcgcgagaa gatgatctgc gtcttcgagc ggtacgccgg tgaggtcgcg 1320
tggagacact cggagagccc catcaccggc ttggacataa gggagtcgcg gccgaaggtg 1380
acgctggtgg cgcggatggt tgcgtccgtg gccaactacg actacatcat ggattgggag 1440
ttccagatgg acggtctcgt ccgcattaag gttgggctga gcgggatcct gatggtgaag 1500
ggcacgtcct actcccacct gagcgaggcc cgcgggaacg agggcgacat gcacggcacg 1560
ctgctctcgg agaacgtgat cggcgtcatc cacgaccact acgtgacctt ccgcctggac 1620
atggacgtcg acggcgccga caactcgttc gttcgcgtgg agatggcgcg gcaggagacg 1680
ggccccggcg atgagtcgcc ccggaggagc tacctcaggg ccacccgccg cgtggcggag 1740
accgagaagg acgcgcgggt gcgcctcagc ctctaccacc cggcggagtt ccacgtcgtc 1800
aaccccgcca agaagacgcg cgtcggcaac cccgttggct acaaggtcgt ccctgccggc 1860
accgccgcga gcttgctgga cccggaggat ccgccgcaga agaggggtgc tttcacaaac 1920
aatcagatat gggtgacgcc ctacaacaag agcgaggaat gggccggcgg cctgttcgtg 1980
taccagagca aaggggagga cactctggat acttggtccg agagagaccg tccgatcgag 2040
aacaaggacc tggtgctgtg gtacacgctg gggttccacc acatcccgtg ccaggaggac 2100
ttccccatca tgcccaccgt gtcgtcaagc ttcgacctca agccagtcaa cttcttcgag 2160
agcaacccca tcctcaagca gcgcccgacc aaagaggatg atctgcccat ctgtactgcc 2220
tccaccgccg cgtag 2235
<210> 124
<211> 744
<212> PRT
<213> Zea mays
<400> 124
Met Asp Arg Ser Thr Ser Leu Leu Arg Leu Ile Leu Leu Ala Leu Gly
1 5 10 15
Ala Ala Leu Val Leu Leu Val Val Arg Ser Ala Phe Arg Leu Pro Arg
20 25 30
Gly Ile Asp Thr Pro Thr Thr Ser Leu Phe Asp Asp Glu Ala Thr Ala
35 40 45
Gly Ser Ser Cys Thr Arg Phe Ala Pro Trp Gly Cys Arg Gln Ala Asp
50 55 60
Arg Ile Lys Gln Lys Pro Pro Ser His Glu Asn Asp Val Pro Arg His
65 70 75 80
Pro Leu Asp Pro Leu Thr Ile Ser Glu Val Asn Arg Ala Arg Glu Leu
85 90 95
Leu Arg Ala His Pro Pro Phe Ala Ser Ser Pro Ser Ser Met Phe Val
100 105 110
His Ser Leu Ala Leu Asp Glu Pro Asp Lys Pro Val Val Leu Ser Trp
115 120 125
Arg Lys Gly Val Asp Pro Leu Pro Pro Arg Arg Ala Val Ala Val Val
130 135 140
Arg Phe Arg Gly Glu Ala Phe Val Leu Ala Ile Asp Leu Ala Ser Gly
145 150 155 160
Ala Val Thr Pro Leu Pro Val Pro Ala Ser Gly Tyr Pro Thr Met Thr
165 170 175
Met Asp Glu Gln Val Leu Leu Cys Tyr Thr Pro Phe Arg Asp Pro Ala
180 185 190
Phe Asn Ala Thr Ile Gln Arg His Gly Val Arg Leu Ser Asp Val Ala
195 200 205
Cys Leu Pro Ile Ser Leu Gly Trp Tyr Gly Pro Ser Glu Glu Asn Arg
210 215 220
Arg Leu Ile Lys Ile Gln Cys Phe Ser Ala Glu Gly Thr Ala Asn Phe
225 230 235 240
Tyr Met Arg Pro Ile Glu Gly Leu Thr Val Leu Val Asp Met Asp Thr
245 250 255
Arg Glu Val Val Arg Ile Ser Asp Arg Gly Ala Gly Ile Pro Ile Pro
260 265 270
Pro Ala Ala Asn Thr Asp Tyr Arg Tyr Ser Arg His Met Gln Asp Glu
275 280 285
Gly Asp Asp Gln Gln Thr Ala Gly Phe Gln Lys Val Arg Ala Pro Ser
290 295 300
Met Glu Pro Gly Pro Ser Ser Gly Pro Arg Val Glu Leu Val Asp Gly
305 310 315 320
His Thr Val Arg Trp Gly Gly Trp Glu Phe His Leu Lys Ala Asp Ala
325 330 335
Arg Ala Gly Met Val Val Ser Arg Ala Arg Val Gln Asp Pro Gly Thr
340 345 350
Gly Ala His Arg Glu Val Leu Tyr Lys Gly Met Ala Ser Glu Leu Phe
355 360 365
Val Pro Tyr Met Asp Pro Thr Glu Ala Trp Tyr Phe Lys Thr Tyr Met
370 375 380
Asp Ala Gly Glu Tyr Gly Phe Gly Leu Gln Ala Met Pro Leu Val Pro
385 390 395 400
Leu Asn Asp Cys Pro Arg His Ala Arg Tyr Leu Asp Gly Val Phe Val
405 410 415
Ala Ala Asp Gly Arg Pro Tyr Val Arg Glu Lys Met Ile Cys Val Phe
420 425 430
Glu Arg Tyr Ala Gly Glu Val Ala Trp Arg His Ser Glu Ser Pro Ile
435 440 445
Thr Gly Leu Asp Ile Arg Glu Ser Arg Pro Lys Val Thr Leu Val Ala
450 455 460
Arg Met Val Ala Ser Val Ala Asn Tyr Asp Tyr Ile Met Asp Trp Glu
465 470 475 480
Phe Gln Met Asp Gly Leu Val Arg Ile Lys Val Gly Leu Ser Gly Ile
485 490 495
Leu Met Val Lys Gly Thr Ser Tyr Ser His Leu Ser Glu Ala Arg Gly
500 505 510
Asn Glu Gly Asp Met His Gly Thr Leu Leu Ser Glu Asn Val Ile Gly
515 520 525
Val Ile His Asp His Tyr Val Thr Phe Arg Leu Asp Met Asp Val Asp
530 535 540
Gly Ala Asp Asn Ser Phe Val Arg Val Glu Met Ala Arg Gln Glu Thr
545 550 555 560
Gly Pro Gly Asp Glu Ser Pro Arg Arg Ser Tyr Leu Arg Ala Thr Arg
565 570 575
Arg Val Ala Glu Thr Glu Lys Asp Ala Arg Val Arg Leu Ser Leu Tyr
580 585 590
His Pro Ala Glu Phe His Val Val Asn Pro Ala Lys Lys Thr Arg Val
595 600 605
Gly Asn Pro Val Gly Tyr Lys Val Val Pro Ala Gly Thr Ala Ala Ser
610 615 620
Leu Leu Asp Pro Glu Asp Pro Pro Gln Lys Arg Gly Ala Phe Thr Asn
625 630 635 640
Asn Gln Ile Trp Val Thr Pro Tyr Asn Lys Ser Glu Glu Trp Ala Gly
645 650 655
Gly Leu Phe Val Tyr Gln Ser Lys Gly Glu Asp Thr Leu Asp Thr Trp
660 665 670
Ser Glu Arg Asp Arg Pro Ile Glu Asn Lys Asp Leu Val Leu Trp Tyr
675 680 685
Thr Leu Gly Phe His His Ile Pro Cys Gln Glu Asp Phe Pro Ile Met
690 695 700
Pro Thr Val Ser Ser Ser Phe Asp Leu Lys Pro Val Asn Phe Phe Glu
705 710 715 720
Ser Asn Pro Ile Leu Lys Gln Arg Pro Thr Lys Glu Asp Asp Leu Pro
725 730 735
Ile Cys Thr Ala Ser Thr Ala Ala
740
<210> 125
<211> 2259
<212> DNA
<213> Sorghum bicolor
<400> 125
atggatcact ccacctccct gctccggctc atcttccttg ctcttggcgc agccctggtt 60
ctccttatcg tccgctccgc cttccgcctc ccacgtggaa tcgacacacc caccacctca 120
ctcttcgatg acgccaccgc cggcagcagc tgcacacggt tcgcgccatg ggggtgccgc 180
caggccgacc ggattaagca ggagcagaag ccgaagccgg agccgccgtc gcacgagaac 240
gacgtgccgc tgcacccgct cgacccactg acggtcaccg agataaaccg tgcgcgcgag 300
ctcctccgcg cgcacccgcc gttcgcgtcg tcgccgtcgt ccatgttcgt gcactcgctg 360
gcgctcgacg agccggacaa gcccgtcgtc ctgagctggc ggaagggcgc cgacccgctg 420
cccccgcgac gcgccgtggc ggtggtccgg ttccgcggcg aggccttcgt cctcgccatc 480
gacctcgcca gcggcgccgt gactcctctg cctgccccag cttccgggta cccgaccatg 540
accatggacg agcaggtgtc cctctgctat gcccctttca gtgacccggc gttcaacgcc 600
accgtccagc ggcacggcgt ccgtatgtcc gacgtcgcct gcctgccgat ctccctcggg 660
tggtacggcc ccaccgagga gaaccgccgg ctgatcaaga tccagtgctt ctccgcggag 720
ggcacggcca acttctacat gcgccccatc gagggcctca ccgtgctgct ggacatggac 780
acgagggagg tcatccgcat ctccgaccgc ggcggcggca tccccatccc gcccgccgcc 840
aacaccgact acaggtatgc ccgccacatg caagaagacg tcggcggcga ccagacgacg 900
tcgaggagtg aggcggggtt ccagaaggtg cgggcgccgt cgatggagcc ggggccgtcg 960
tcggggccgg gagtggagct ggtggacggg cacacggttc ggtggggcgg gtgggagttc 1020
cacctcaagg cggacgcgcg cgccggcatg gtggtgtcgc gcgcacgggt gcaggacccc 1080
ggcacgggcg cgcaccggga ggtgctgtac aagggcatgg cgtcggagct cttcgtgccg 1140
tacatggacc cgaccgaggc gtggtacttc aagacgtaca tggatgccgg cgagtacggc 1200
ttcggcctgc aggccatgcc gctggtgccg ctcaacgact gcccacgcca cgcacggtac 1260
ctcgacggcg tgttcgtggc cgccgacggc cggccctacg tgcgggagaa gatgatctgc 1320
gtcttcgagc ggtacgccgg cgaggtcgcg tggagacact cggagagccc catcaccggc 1380
atggacataa gggagtcgcg gccgaaggtg acgctggtgg cgcggatggt tgcgtccgtg 1440
gccaactacg actacatcat ggactgggag ttccagatgg acggcctcgt ccgcatcaag 1500
gtgggcctga gcgggatcct gatggtgaag ggcacggcct actcccacct acgggaggcc 1560
cgcgagaacg aggacacgca cggcacgctg ctctccgaga acgtcatcgg cgtcatccac 1620
gaccactacg tgacgttccg cctggacatg gacgtcgacg gcgccgacaa caactccttc 1680
gtgcgcgtgg agatggcgcg gcaggagacg gcccccggcg agtccccccg gaggagctac 1740
ctcaaggcca cccggcacgt ggcgcggacc gagaaggacg cccaggtgcg cctcaagctc 1800
tacgatccgg ccgagttcca tgtcgtcaac ccgaccaaga agacgcgggt cggcaacccc 1860
gttggctaca agctcgtccc tgccggcacc gccgccagct tgctggaccc ggaggatccg 1920
ccgcagaaga ggggtgcttt cacaaacaat cagatctggg tgacacccta caacaagagc 1980
gaggaatggg ccggtggtct gttcgtgtac cagagcaaag gggaggacac attggctact 2040
tggtccgaga gagaccgtcc gatcgagaac aaggacctgg tgctgtggta cacgctgggg 2100
ttccaccaca tcccgtgcca ggaggacttc cccatcatgc ccaccgtgtc ctcaagcttc 2160
gacctcaagc ctgtcaactt cttcgagagc aaccccatcc tcaagcagag gcccaccaag 2220
gaggatgatc tgcccatctg ttccgccacc gccgtgtaa 2259
<210> 126
<211> 752
<212> PRT
<213> Sorghum bicolor
<400> 126
Met Asp His Ser Thr Ser Leu Leu Arg Leu Ile Phe Leu Ala Leu Gly
1 5 10 15
Ala Ala Leu Val Leu Leu Ile Val Arg Ser Ala Phe Arg Leu Pro Arg
20 25 30
Gly Ile Asp Thr Pro Thr Thr Ser Leu Phe Asp Asp Ala Thr Ala Gly
35 40 45
Ser Ser Cys Thr Arg Phe Ala Pro Trp Gly Cys Arg Gln Ala Asp Arg
50 55 60
Ile Lys Gln Glu Gln Lys Pro Lys Pro Glu Pro Pro Ser His Glu Asn
65 70 75 80
Asp Val Pro Leu His Pro Leu Asp Pro Leu Thr Val Thr Glu Ile Asn
85 90 95
Arg Ala Arg Glu Leu Leu Arg Ala His Pro Pro Phe Ala Ser Ser Pro
100 105 110
Ser Ser Met Phe Val His Ser Leu Ala Leu Asp Glu Pro Asp Lys Pro
115 120 125
Val Val Leu Ser Trp Arg Lys Gly Ala Asp Pro Leu Pro Pro Arg Arg
130 135 140
Ala Val Ala Val Val Arg Phe Arg Gly Glu Ala Phe Val Leu Ala Ile
145 150 155 160
Asp Leu Ala Ser Gly Ala Val Thr Pro Leu Pro Ala Pro Ala Ser Gly
165 170 175
Tyr Pro Thr Met Thr Met Asp Glu Gln Val Ser Leu Cys Tyr Ala Pro
180 185 190
Phe Ser Asp Pro Ala Phe Asn Ala Thr Val Gln Arg His Gly Val Arg
195 200 205
Met Ser Asp Val Ala Cys Leu Pro Ile Ser Leu Gly Trp Tyr Gly Pro
210 215 220
Thr Glu Glu Asn Arg Arg Leu Ile Lys Ile Gln Cys Phe Ser Ala Glu
225 230 235 240
Gly Thr Ala Asn Phe Tyr Met Arg Pro Ile Glu Gly Leu Thr Val Leu
245 250 255
Leu Asp Met Asp Thr Arg Glu Val Ile Arg Ile Ser Asp Arg Gly Gly
260 265 270
Gly Ile Pro Ile Pro Pro Ala Ala Asn Thr Asp Tyr Arg Tyr Ala Arg
275 280 285
His Met Gln Glu Asp Val Gly Gly Asp Gln Thr Thr Ser Arg Ser Glu
290 295 300
Ala Gly Phe Gln Lys Val Arg Ala Pro Ser Met Glu Pro Gly Pro Ser
305 310 315 320
Ser Gly Pro Gly Val Glu Leu Val Asp Gly His Thr Val Arg Trp Gly
325 330 335
Gly Trp Glu Phe His Leu Lys Ala Asp Ala Arg Ala Gly Met Val Val
340 345 350
Ser Arg Ala Arg Val Gln Asp Pro Gly Thr Gly Ala His Arg Glu Val
355 360 365
Leu Tyr Lys Gly Met Ala Ser Glu Leu Phe Val Pro Tyr Met Asp Pro
370 375 380
Thr Glu Ala Trp Tyr Phe Lys Thr Tyr Met Asp Ala Gly Glu Tyr Gly
385 390 395 400
Phe Gly Leu Gln Ala Met Pro Leu Val Pro Leu Asn Asp Cys Pro Arg
405 410 415
His Ala Arg Tyr Leu Asp Gly Val Phe Val Ala Ala Asp Gly Arg Pro
420 425 430
Tyr Val Arg Glu Lys Met Ile Cys Val Phe Glu Arg Tyr Ala Gly Glu
435 440 445
Val Ala Trp Arg His Ser Glu Ser Pro Ile Thr Gly Met Asp Ile Arg
450 455 460
Glu Ser Arg Pro Lys Val Thr Leu Val Ala Arg Met Val Ala Ser Val
465 470 475 480
Ala Asn Tyr Asp Tyr Ile Met Asp Trp Glu Phe Gln Met Asp Gly Leu
485 490 495
Val Arg Ile Lys Val Gly Leu Ser Gly Ile Leu Met Val Lys Gly Thr
500 505 510
Ala Tyr Ser His Leu Arg Glu Ala Arg Glu Asn Glu Asp Thr His Gly
515 520 525
Thr Leu Leu Ser Glu Asn Val Ile Gly Val Ile His Asp His Tyr Val
530 535 540
Thr Phe Arg Leu Asp Met Asp Val Asp Gly Ala Asp Asn Asn Ser Phe
545 550 555 560
Val Arg Val Glu Met Ala Arg Gln Glu Thr Ala Pro Gly Glu Ser Pro
565 570 575
Arg Arg Ser Tyr Leu Lys Ala Thr Arg His Val Ala Arg Thr Glu Lys
580 585 590
Asp Ala Gln Val Arg Leu Lys Leu Tyr Asp Pro Ala Glu Phe His Val
595 600 605
Val Asn Pro Thr Lys Lys Thr Arg Val Gly Asn Pro Val Gly Tyr Lys
610 615 620
Leu Val Pro Ala Gly Thr Ala Ala Ser Leu Leu Asp Pro Glu Asp Pro
625 630 635 640
Pro Gln Lys Arg Gly Ala Phe Thr Asn Asn Gln Ile Trp Val Thr Pro
645 650 655
Tyr Asn Lys Ser Glu Glu Trp Ala Gly Gly Leu Phe Val Tyr Gln Ser
660 665 670
Lys Gly Glu Asp Thr Leu Ala Thr Trp Ser Glu Arg Asp Arg Pro Ile
675 680 685
Glu Asn Lys Asp Leu Val Leu Trp Tyr Thr Leu Gly Phe His His Ile
690 695 700
Pro Cys Gln Glu Asp Phe Pro Ile Met Pro Thr Val Ser Ser Ser Phe
705 710 715 720
Asp Leu Lys Pro Val Asn Phe Phe Glu Ser Asn Pro Ile Leu Lys Gln
725 730 735
Arg Pro Thr Lys Glu Asp Asp Leu Pro Ile Cys Ser Ala Thr Ala Val
740 745 750
<210> 127
<211> 2277
<212> DNA
<213> Oryza sativa
<400> 127
atggcttccc tcctcacgct cccctccctc tccctctcca accctagcgc ctccgccgcc 60
gccgcgggag ccggagcggc gccgtctctg cgcctccgcg ccgccttccg ctgctgggcg 120
ctgcggcgcg cgggcggcgg ccggtgggcg gccgcggggg ccatcgcgtc gcccaactcg 180
gtgctcagcg agcacgcgtt caagcggctg cagctcagcg acgaggagga ggaggaggag 240
gagggggcct acgggagtga cgaggagggg gtcgaggcgg tgggtggcgg ggagggggac 300
gaggacgagc tcgccattgc caggctcggc ctgcccgagc agctcgtatc cacgctcgag 360
aagcgcggga ttacccacct gttccccatc cagagggctg tattgattcc agctcttgac 420
ggccgtgacc tgattgctag agcaaagaca ggaaccggga agacgctagc cttcggcatt 480
cccatgatca agcaattaat ggaggaggat gatggacgga gtgtgaggcg aggtcgtatt 540
cctcgtgttt tggttctagc acctactagg gagttagcca agcaagttga gaaggaaata 600
aaagaatcag cacccaagct cagtacagtc tgtgtttatg gaggtgtctc atataatgtc 660
cagcagaacg cactctcccg tggtgttgat gttgttgtag gaacacctgg tcgcattatt 720
gatttgataa atggcggaag tcttcagttg ggagaagtta agtacttggt ccttgatgag 780
gctgaccaaa tgcttgcagt tgggtttgag gaggatgtgg aaacgatatt acaacagcta 840
ccagctgaga ggcaaagcat gctcttctct gccaccatgc ctggttgggt gaagaaatta 900
tctaggcgat acttgaacaa tcctttgaca attgacttgg tcggcgatca agatgaaaaa 960
cttgctgaag gaatcaaact ttatgctatc ccgctcacat ctacatcgaa gcgcactgtt 1020
cttagtgatc tcattacggt gtatgcaaag ggtgggaaaa ccattgtgtt caccaagacg 1080
aaaagggatg cagacgaggt atcattagca ctgacaaaca gtattgcttc ggaggcactg 1140
catggtgata tttcacagca tcagcgtgag aggacactaa atggttttcg tcaagggaaa 1200
tttactgttc tcgtagcaac cgatgttgct gcccgtggtc ttgatatacc gaatgttgat 1260
ttgattatcc attatgaatt gcccaatgat ccagagactt ttgttcatcg ttctggacgc 1320
actggccgag ctgggaaagc aggaactgca atcttgatgt tcacaaacag ccagagaagg 1380
acagttagat cacttgagcg tgatgttgga tgcagatttg acttcataag tcctccagca 1440
attgaggatg tgctggagtc ctctgctgaa catgtcatag ctactttaag aggtgtgcac 1500
acggagtcga ttcagtactt cattccagca gctgaaagac tgcaagaaga attaggacct 1560
aatgctcttg cttctgcatt ggcacatctg agtggatttt ctcagccacc ctcttcacgt 1620
tctcttatca gtcatgagca gggatgggtg actttgcaac taactagaga tccaggatat 1680
ggaagggggt tcttttctcc tagatctgtt actggctttc tgtctgatgt ttcttcagct 1740
gctgctgatg aagttggcaa aattttcctt acagcagatg agaaggttca aggtgcagtt 1800
ttcgatttac ctgaggagat tgcgagggat ttgcttagta tggaactgcc cccaggaaac 1860
accataacca aagtgacaaa gctacctgca ttacaggatg atggtcctgc tactgatagt 1920
tatggccgat tctcaaactc ggaccggggt ttcaggaaca gacggtccag gggtggtggc 1980
tcaagaggcg ggcggggtgg ttgggactct gatggtgaag acagatttcg ccgtggtggc 2040
aggagcttca gatctgacaa tgatagttgg tcagatgatg actttggtgg cgggagaaga 2100
tcgaaccgtt cgtcatcctt tggtggccgc gggtcatctt acggcagtcg tagctcgtca 2160
tcctttggtg gccgctcatc atcttttggg tctagggaca gcagcaggag cttcagtggt 2220
gcttgcttca actgtggcga aagcgggcac cgagcatcag actgcccaaa caagtag 2277
<210> 128
<211> 758
<212> PRT
<213> Oryza sativa
<400> 128
Met Ala Ser Leu Leu Thr Leu Pro Ser Leu Ser Leu Ser Asn Pro Ser
1 5 10 15
Ala Ser Ala Ala Ala Ala Gly Ala Gly Ala Ala Pro Ser Leu Arg Leu
20 25 30
Arg Ala Ala Phe Arg Cys Trp Ala Leu Arg Arg Ala Gly Gly Gly Arg
35 40 45
Trp Ala Ala Ala Gly Ala Ile Ala Ser Pro Asn Ser Val Leu Ser Glu
50 55 60
His Ala Phe Lys Arg Leu Gln Leu Ser Asp Glu Glu Glu Glu Glu Glu
65 70 75 80
Glu Gly Ala Tyr Gly Ser Asp Glu Glu Gly Val Glu Ala Val Gly Gly
85 90 95
Gly Glu Gly Asp Glu Asp Glu Leu Ala Ile Ala Arg Leu Gly Leu Pro
100 105 110
Glu Gln Leu Val Ser Thr Leu Glu Lys Arg Gly Ile Thr His Leu Phe
115 120 125
Pro Ile Gln Arg Ala Val Leu Ile Pro Ala Leu Asp Gly Arg Asp Leu
130 135 140
Ile Ala Arg Ala Lys Thr Gly Thr Gly Lys Thr Leu Ala Phe Gly Ile
145 150 155 160
Pro Met Ile Lys Gln Leu Met Glu Glu Asp Asp Gly Arg Ser Val Arg
165 170 175
Arg Gly Arg Ile Pro Arg Val Leu Val Leu Ala Pro Thr Arg Glu Leu
180 185 190
Ala Lys Gln Val Glu Lys Glu Ile Lys Glu Ser Ala Pro Lys Leu Ser
195 200 205
Thr Val Cys Val Tyr Gly Gly Val Ser Tyr Asn Val Gln Gln Asn Ala
210 215 220
Leu Ser Arg Gly Val Asp Val Val Val Gly Thr Pro Gly Arg Ile Ile
225 230 235 240
Asp Leu Ile Asn Gly Gly Ser Leu Gln Leu Gly Glu Val Lys Tyr Leu
245 250 255
Val Leu Asp Glu Ala Asp Gln Met Leu Ala Val Gly Phe Glu Glu Asp
260 265 270
Val Glu Thr Ile Leu Gln Gln Leu Pro Ala Glu Arg Gln Ser Met Leu
275 280 285
Phe Ser Ala Thr Met Pro Gly Trp Val Lys Lys Leu Ser Arg Arg Tyr
290 295 300
Leu Asn Asn Pro Leu Thr Ile Asp Leu Val Gly Asp Gln Asp Glu Lys
305 310 315 320
Leu Ala Glu Gly Ile Lys Leu Tyr Ala Ile Pro Leu Thr Ser Thr Ser
325 330 335
Lys Arg Thr Val Leu Ser Asp Leu Ile Thr Val Tyr Ala Lys Gly Gly
340 345 350
Lys Thr Ile Val Phe Thr Lys Thr Lys Arg Asp Ala Asp Glu Val Ser
355 360 365
Leu Ala Leu Thr Asn Ser Ile Ala Ser Glu Ala Leu His Gly Asp Ile
370 375 380
Ser Gln His Gln Arg Glu Arg Thr Leu Asn Gly Phe Arg Gln Gly Lys
385 390 395 400
Phe Thr Val Leu Val Ala Thr Asp Val Ala Ala Arg Gly Leu Asp Ile
405 410 415
Pro Asn Val Asp Leu Ile Ile His Tyr Glu Leu Pro Asn Asp Pro Glu
420 425 430
Thr Phe Val His Arg Ser Gly Arg Thr Gly Arg Ala Gly Lys Ala Gly
435 440 445
Thr Ala Ile Leu Met Phe Thr Asn Ser Gln Arg Arg Thr Val Arg Ser
450 455 460
Leu Glu Arg Asp Val Gly Cys Arg Phe Asp Phe Ile Ser Pro Pro Ala
465 470 475 480
Ile Glu Asp Val Leu Glu Ser Ser Ala Glu His Val Ile Ala Thr Leu
485 490 495
Arg Gly Val His Thr Glu Ser Ile Gln Tyr Phe Ile Pro Ala Ala Glu
500 505 510
Arg Leu Gln Glu Glu Leu Gly Pro Asn Ala Leu Ala Ser Ala Leu Ala
515 520 525
His Leu Ser Gly Phe Ser Gln Pro Pro Ser Ser Arg Ser Leu Ile Ser
530 535 540
His Glu Gln Gly Trp Val Thr Leu Gln Leu Thr Arg Asp Pro Gly Tyr
545 550 555 560
Gly Arg Gly Phe Phe Ser Pro Arg Ser Val Thr Gly Phe Leu Ser Asp
565 570 575
Val Ser Ser Ala Ala Ala Asp Glu Val Gly Lys Ile Phe Leu Thr Ala
580 585 590
Asp Glu Lys Val Gln Gly Ala Val Phe Asp Leu Pro Glu Glu Ile Ala
595 600 605
Arg Asp Leu Leu Ser Met Glu Leu Pro Pro Gly Asn Thr Ile Thr Lys
610 615 620
Val Thr Lys Leu Pro Ala Leu Gln Asp Asp Gly Pro Ala Thr Asp Ser
625 630 635 640
Tyr Gly Arg Phe Ser Asn Ser Asp Arg Gly Phe Arg Asn Arg Arg Ser
645 650 655
Arg Gly Gly Gly Ser Arg Gly Gly Arg Gly Gly Trp Asp Ser Asp Gly
660 665 670
Glu Asp Arg Phe Arg Arg Gly Gly Arg Ser Phe Arg Ser Asp Asn Asp
675 680 685
Ser Trp Ser Asp Asp Asp Phe Gly Gly Gly Arg Arg Ser Asn Arg Ser
690 695 700
Ser Ser Phe Gly Gly Arg Gly Ser Ser Tyr Gly Ser Arg Ser Ser Ser
705 710 715 720
Ser Phe Gly Gly Arg Ser Ser Ser Phe Gly Ser Arg Asp Ser Ser Arg
725 730 735
Ser Phe Ser Gly Ala Cys Phe Asn Cys Gly Glu Ser Gly His Arg Ala
740 745 750
Ser Asp Cys Pro Asn Lys
755
<210> 129
<211> 2115
<212> DNA
<213> Zea mays
<400> 129
atgtctcccg ccctcgccgc tgccgtggag cccatggccg tcgacgactc cgcctccaag 60
aaggccaagc gcaagcagct caaagccgct gccgccgccg ccgccgcgga ggcagaggag 120
gaggctgctt cggccaagaa gaaagagaag aaggagaaga agcggaaagc gaaggagccg 180
tcccctccgc tcccgtcggc cgcatcatct ggcgaggaga agagcagcac cagctccgag 240
gagacagccc ccaccgcgaa gaaggcgaag aaggacaaga cgaagaagaa tgtcgaggtc 300
tcgtcttcag cctcagatga cgacggcgag atcacggccg gcagcgacga ggaccccgcg 360
gacccgaacg cgctgacgaa cttcaggata tcggagccac tgaggcagag cctcaggtcc 420
aaggggatca aggcactgtt ccccatccag gccaccactt tcgacctcgt actcgacggc 480
agcgacttgg ttggccgagc gcgcaccggt cagggaaaaa ctttggcttt tgtcttgccc 540
atattggaat ctttggttaa cggggcaaat aaggcatcta ggcggactga acatggcagg 600
accccaagtg ttctcgttct gctaccaaca agagagctgg ccaatcaggt gcatgccgac 660
tttgagtttt atggtgcaac atttgggctt tctgcatgtt gtgtgtatgg gggttcacct 720
tatcgtcctc aagaaatggc attgagaaga ggcgtggaca ttgttgttgg aactcctggt 780
cgtatcaagg attttattgt aaaaggaact ctcaacttga aatgcttgaa gttccgtgtc 840
cttgacgaag ctgatgagat gcttaacatg ggctttgttg atgatgtcga gctcattctt 900
ggcaaggtag aagatgctac caaagtacag acacttctgt tcagtgccac tctgccagat 960
tgggtgaata agctctctat gaggtttctg aaagttgaca ggaaaacagt tgatcttgtc 1020
ggtaacgaga aactgaaggc cagtgcatct gttaagcacc ttgctcttcc ttgtaacaag 1080
gcagcaaggg cacaacttat tccagatatt atccgatgtt acagccatgg aggccgaacc 1140
attatcttca ctgagacgaa ggattctgca tcagagcttt ctggtttgat tcctggatcc 1200
cgtgccttgc atggagatgt tgtgcaagct cagcgtgaag tcattcttgc tggatttcgt 1260
agcgggaagt tccaggtttt ggttgctaca aatgtggcgg ctcgtggtct ggatattaat 1320
gatgtgcagc ttatcattca gtgtgaacct ccccgtgacg ttgaagctta catacaccgg 1380
tcaggtcgga cagggagagc aggtaatact ggtgttgctg tcatgcttta tgagcccaga 1440
tataagtaca gtgtcagcag actagaaagg gaatctgggg ttaagttcga acatatctct 1500
gcaccacaac ctactgatgt agcacaatct gctggcagtg aagctgcaga tgccattgcg 1560
agtgtgtcag acagtgttat tcctgtcttc aggcagcaag cagagcagtt gctaagctct 1620
tccactctgt ctgcggctga cttgcttgcc aaagctcttg caaaggcagt tggttacacg 1680
gacataaaga aaaggtcatt gttgtcttca atggaggatt acgctacact acatcttcaa 1740
actggcagac agatgtggtc acctgggttt gcttttacta tattgaaaag gttcatgcca 1800
gaagagaaac ttgcagatgt aaagggtgca accctcacgg ctgatggaac gggggttgta 1860
tttgatgttc ctgcagcaga tgttgaagat tacattcaag cttcggagaa tgccgcacag 1920
gtgacaattg atgaagtcca gcaattgcca cccttgcaag agaagcagca gcagcagtca 1980
agaggcaact cggggggagg aagatttggc cgtggaggtg gcgggagatt ctctggtggt 2040
ggccgtgggg gcggcttcgg tggtggcgga agaggcagag gtggtggtgg cggcaggggg 2100
aggcggcggc aatag 2115
<210> 130
<211> 704
<212> PRT
<213> Zea mays
<400> 130
Met Ser Pro Ala Leu Ala Ala Ala Val Glu Pro Met Ala Val Asp Asp
1 5 10 15
Ser Ala Ser Lys Lys Ala Lys Arg Lys Gln Leu Lys Ala Ala Ala Ala
20 25 30
Ala Ala Ala Ala Glu Ala Glu Glu Glu Ala Ala Ser Ala Lys Lys Lys
35 40 45
Glu Lys Lys Glu Lys Lys Arg Lys Ala Lys Glu Pro Ser Pro Pro Leu
50 55 60
Pro Ser Ala Ala Ser Ser Gly Glu Glu Lys Ser Ser Thr Ser Ser Glu
65 70 75 80
Glu Thr Ala Pro Thr Ala Lys Lys Ala Lys Lys Asp Lys Thr Lys Lys
85 90 95
Asn Val Glu Val Ser Ser Ser Ala Ser Asp Asp Asp Gly Glu Ile Thr
100 105 110
Ala Gly Ser Asp Glu Asp Pro Ala Asp Pro Asn Ala Leu Thr Asn Phe
115 120 125
Arg Ile Ser Glu Pro Leu Arg Gln Ser Leu Arg Ser Lys Gly Ile Lys
130 135 140
Ala Leu Phe Pro Ile Gln Ala Thr Thr Phe Asp Leu Val Leu Asp Gly
145 150 155 160
Ser Asp Leu Val Gly Arg Ala Arg Thr Gly Gln Gly Lys Thr Leu Ala
165 170 175
Phe Val Leu Pro Ile Leu Glu Ser Leu Val Asn Gly Ala Asn Lys Ala
180 185 190
Ser Arg Arg Thr Glu His Gly Arg Thr Pro Ser Val Leu Val Leu Leu
195 200 205
Pro Thr Arg Glu Leu Ala Asn Gln Val His Ala Asp Phe Glu Phe Tyr
210 215 220
Gly Ala Thr Phe Gly Leu Ser Ala Cys Cys Val Tyr Gly Gly Ser Pro
225 230 235 240
Tyr Arg Pro Gln Glu Met Ala Leu Arg Arg Gly Val Asp Ile Val Val
245 250 255
Gly Thr Pro Gly Arg Ile Lys Asp Phe Ile Val Lys Gly Thr Leu Asn
260 265 270
Leu Lys Cys Leu Lys Phe Arg Val Leu Asp Glu Ala Asp Glu Met Leu
275 280 285
Asn Met Gly Phe Val Asp Asp Val Glu Leu Ile Leu Gly Lys Val Glu
290 295 300
Asp Ala Thr Lys Val Gln Thr Leu Leu Phe Ser Ala Thr Leu Pro Asp
305 310 315 320
Trp Val Asn Lys Leu Ser Met Arg Phe Leu Lys Val Asp Arg Lys Thr
325 330 335
Val Asp Leu Val Gly Asn Glu Lys Leu Lys Ala Ser Ala Ser Val Lys
340 345 350
His Leu Ala Leu Pro Cys Asn Lys Ala Ala Arg Ala Gln Leu Ile Pro
355 360 365
Asp Ile Ile Arg Cys Tyr Ser His Gly Gly Arg Thr Ile Ile Phe Thr
370 375 380
Glu Thr Lys Asp Ser Ala Ser Glu Leu Ser Gly Leu Ile Pro Gly Ser
385 390 395 400
Arg Ala Leu His Gly Asp Val Val Gln Ala Gln Arg Glu Val Ile Leu
405 410 415
Ala Gly Phe Arg Ser Gly Lys Phe Gln Val Leu Val Ala Thr Asn Val
420 425 430
Ala Ala Arg Gly Leu Asp Ile Asn Asp Val Gln Leu Ile Ile Gln Cys
435 440 445
Glu Pro Pro Arg Asp Val Glu Ala Tyr Ile His Arg Ser Gly Arg Thr
450 455 460
Gly Arg Ala Gly Asn Thr Gly Val Ala Val Met Leu Tyr Glu Pro Arg
465 470 475 480
Tyr Lys Tyr Ser Val Ser Arg Leu Glu Arg Glu Ser Gly Val Lys Phe
485 490 495
Glu His Ile Ser Ala Pro Gln Pro Thr Asp Val Ala Gln Ser Ala Gly
500 505 510
Ser Glu Ala Ala Asp Ala Ile Ala Ser Val Ser Asp Ser Val Ile Pro
515 520 525
Val Phe Arg Gln Gln Ala Glu Gln Leu Leu Ser Ser Ser Thr Leu Ser
530 535 540
Ala Ala Asp Leu Leu Ala Lys Ala Leu Ala Lys Ala Val Gly Tyr Thr
545 550 555 560
Asp Ile Lys Lys Arg Ser Leu Leu Ser Ser Met Glu Asp Tyr Ala Thr
565 570 575
Leu His Leu Gln Thr Gly Arg Gln Met Trp Ser Pro Gly Phe Ala Phe
580 585 590
Thr Ile Leu Lys Arg Phe Met Pro Glu Glu Lys Leu Ala Asp Val Lys
595 600 605
Gly Ala Thr Leu Thr Ala Asp Gly Thr Gly Val Val Phe Asp Val Pro
610 615 620
Ala Ala Asp Val Glu Asp Tyr Ile Gln Ala Ser Glu Asn Ala Ala Gln
625 630 635 640
Val Thr Ile Asp Glu Val Gln Gln Leu Pro Pro Leu Gln Glu Lys Gln
645 650 655
Gln Gln Gln Ser Arg Gly Asn Ser Gly Gly Gly Arg Phe Gly Arg Gly
660 665 670
Gly Gly Gly Arg Phe Ser Gly Gly Gly Arg Gly Gly Gly Phe Gly Gly
675 680 685
Gly Gly Arg Gly Arg Gly Gly Gly Gly Gly Arg Gly Arg Arg Arg Gln
690 695 700
<210> 131
<211> 2136
<212> DNA
<213> Sorghum bicolor
<400> 131
atgtctcccg cccttgcctc tgccgtggag cccatggccg tcgacgactc cgcctccaag 60
aaggccaagc gcaagcagct caaggccgcc gccgccgccg cggaggcaga ggcggaggcc 120
gcttcggcta agaagaaaga gaagaaggag aagaagcgca aggcgaagga gccgtcccct 180
ccgcccccgt cggcctcgtc ctctgacgag gaggagaaga gcagcaccag ctccgaggag 240
acggcccccg ccgcgaagaa agcgaagaag gagaagacga agaagaatgt cgaggtctcg 300
tcttcagcct cggacgacga cggcgagatc acggccagca gcgacgagga ccccgcggac 360
ccgaacgcgc tgacgaactt caggatatcg gagccgctga ggcagagcct caggtccaag 420
gggatcaagg cgctgttccc catccaggcc accactttcg acctcgtact cgacggcaac 480
gacttggttg gccgagcgcg caccggtcag gggaaaactt tggcttttgt cttgcccata 540
ttggagtctt tggttaacgg ggcacacaag gcatctagac gaactgaaca tggcaggacc 600
ccaagtgtta ttgttctgct acccacaaga gagctggcca atcaggtgca tgccgacttt 660
gagttttatg gtgcaacatt tgggctttcc gcatgttgtg tgtatggggg ttctccttat 720
cgtcctcaag aaatggcttt gagaaggggt gtggacattg ttgttggaac tcctggtcgt 780
gtcaaggatt tcattgtaaa aggaactctc aatttgaaaa acttgaaatt ccgtgtcctt 840
gatgaagctg atgagatgct taacatgggt tttgttgatg atgtcgagct cattcttggc 900
aaagtagaag atgctaccaa agtacagaca cttctgttca gtgccactct gccagattgg 960
gtgaataagc tctctatgag gtttctgaaa gttgacagga aaacggttga tcttgtcggt 1020
aatgagaaac tgaaggccag tgcatctgtt aagcaccttg ctcttccttg taacaaggca 1080
gcaagggcac aagttattcc agacattatc cgatgctata gccatggagg ccgaaccatt 1140
atcttcactg agacaaagga ttctgcatca gagctttctg gtttgattcc tggatcccgt 1200
gccttgcatg gagatgtcgt gcaagctcag cgtgaagtca ttcttgctgg attccgtggc 1260
gggaagttcc aagttttggt tgctacaaat gtggcggctc gtggtctgga tattaatgat 1320
gtgcagctta tcattcagtg tgaacctccc cgtgacgttg aagcttacat acaccggtca 1380
ggtcggacag ggagagcagg taatactggt gttgctgtca tgctttatga gcccagatat 1440
aaatacagtg tcaacagact agaaagggag tctggggtta agtttgagca tatctctgcg 1500
ccacagccta ctgatgtagc acaatctgct ggcagtgaag ctgcagatgc catttcgagt 1560
gtgtcagata gtgttattcc tgtcttcagg cagcaagcag agcagttgct aagctcttcc 1620
agcctgtctg cagctgactt gcttgccaag gcacttgcaa aggcagttgg ttacacggac 1680
ataaagaaaa gatcattgtt atcttccatg gaggattaca ctacattaca tcttcaaact 1740
ggcagaccga tgtggtcacc tgggtttgct tttactatat tgaaaaggtt catgccagaa 1800
gagaaacttg cagatgtaaa gggtgcaacc ctcacggctg atggaacagg tgttgtattt 1860
gatgttcctg cagcagatgt tgaagattac attcaagctt cggagaatgc tgcacaggtg 1920
acaattgatg aagtcaagca actgccaccc ttgcaagaga aggagcagtc aagaggcaac 1980
tctggcggag gaagatttgg ccgtggaggt ggtgggagat tctctggtgg tggccgtgga 2040
ggcggcttcg gtggtggtgg cagaggcaga ggtggtggcg gcagagggtt ttctgggagg 2100
ggaggcggcg gcaacagatt taacaggagg aattag 2136
<210> 132
<211> 711
<212> PRT
<213> Sorghum bicolor
<400> 132
Met Ser Pro Ala Leu Ala Ser Ala Val Glu Pro Met Ala Val Asp Asp
1 5 10 15
Ser Ala Ser Lys Lys Ala Lys Arg Lys Gln Leu Lys Ala Ala Ala Ala
20 25 30
Ala Ala Glu Ala Glu Ala Glu Ala Ala Ser Ala Lys Lys Lys Glu Lys
35 40 45
Lys Glu Lys Lys Arg Lys Ala Lys Glu Pro Ser Pro Pro Pro Pro Ser
50 55 60
Ala Ser Ser Ser Asp Glu Glu Glu Lys Ser Ser Thr Ser Ser Glu Glu
65 70 75 80
Thr Ala Pro Ala Ala Lys Lys Ala Lys Lys Glu Lys Thr Lys Lys Asn
85 90 95
Val Glu Val Ser Ser Ser Ala Ser Asp Asp Asp Gly Glu Ile Thr Ala
100 105 110
Ser Ser Asp Glu Asp Pro Ala Asp Pro Asn Ala Leu Thr Asn Phe Arg
115 120 125
Ile Ser Glu Pro Leu Arg Gln Ser Leu Arg Ser Lys Gly Ile Lys Ala
130 135 140
Leu Phe Pro Ile Gln Ala Thr Thr Phe Asp Leu Val Leu Asp Gly Asn
145 150 155 160
Asp Leu Val Gly Arg Ala Arg Thr Gly Gln Gly Lys Thr Leu Ala Phe
165 170 175
Val Leu Pro Ile Leu Glu Ser Leu Val Asn Gly Ala His Lys Ala Ser
180 185 190
Arg Arg Thr Glu His Gly Arg Thr Pro Ser Val Ile Val Leu Leu Pro
195 200 205
Thr Arg Glu Leu Ala Asn Gln Val His Ala Asp Phe Glu Phe Tyr Gly
210 215 220
Ala Thr Phe Gly Leu Ser Ala Cys Cys Val Tyr Gly Gly Ser Pro Tyr
225 230 235 240
Arg Pro Gln Glu Met Ala Leu Arg Arg Gly Val Asp Ile Val Val Gly
245 250 255
Thr Pro Gly Arg Val Lys Asp Phe Ile Val Lys Gly Thr Leu Asn Leu
260 265 270
Lys Asn Leu Lys Phe Arg Val Leu Asp Glu Ala Asp Glu Met Leu Asn
275 280 285
Met Gly Phe Val Asp Asp Val Glu Leu Ile Leu Gly Lys Val Glu Asp
290 295 300
Ala Thr Lys Val Gln Thr Leu Leu Phe Ser Ala Thr Leu Pro Asp Trp
305 310 315 320
Val Asn Lys Leu Ser Met Arg Phe Leu Lys Val Asp Arg Lys Thr Val
325 330 335
Asp Leu Val Gly Asn Glu Lys Leu Lys Ala Ser Ala Ser Val Lys His
340 345 350
Leu Ala Leu Pro Cys Asn Lys Ala Ala Arg Ala Gln Val Ile Pro Asp
355 360 365
Ile Ile Arg Cys Tyr Ser His Gly Gly Arg Thr Ile Ile Phe Thr Glu
370 375 380
Thr Lys Asp Ser Ala Ser Glu Leu Ser Gly Leu Ile Pro Gly Ser Arg
385 390 395 400
Ala Leu His Gly Asp Val Val Gln Ala Gln Arg Glu Val Ile Leu Ala
405 410 415
Gly Phe Arg Gly Gly Lys Phe Gln Val Leu Val Ala Thr Asn Val Ala
420 425 430
Ala Arg Gly Leu Asp Ile Asn Asp Val Gln Leu Ile Ile Gln Cys Glu
435 440 445
Pro Pro Arg Asp Val Glu Ala Tyr Ile His Arg Ser Gly Arg Thr Gly
450 455 460
Arg Ala Gly Asn Thr Gly Val Ala Val Met Leu Tyr Glu Pro Arg Tyr
465 470 475 480
Lys Tyr Ser Val Asn Arg Leu Glu Arg Glu Ser Gly Val Lys Phe Glu
485 490 495
His Ile Ser Ala Pro Gln Pro Thr Asp Val Ala Gln Ser Ala Gly Ser
500 505 510
Glu Ala Ala Asp Ala Ile Ser Ser Val Ser Asp Ser Val Ile Pro Val
515 520 525
Phe Arg Gln Gln Ala Glu Gln Leu Leu Ser Ser Ser Ser Leu Ser Ala
530 535 540
Ala Asp Leu Leu Ala Lys Ala Leu Ala Lys Ala Val Gly Tyr Thr Asp
545 550 555 560
Ile Lys Lys Arg Ser Leu Leu Ser Ser Met Glu Asp Tyr Thr Thr Leu
565 570 575
His Leu Gln Thr Gly Arg Pro Met Trp Ser Pro Gly Phe Ala Phe Thr
580 585 590
Ile Leu Lys Arg Phe Met Pro Glu Glu Lys Leu Ala Asp Val Lys Gly
595 600 605
Ala Thr Leu Thr Ala Asp Gly Thr Gly Val Val Phe Asp Val Pro Ala
610 615 620
Ala Asp Val Glu Asp Tyr Ile Gln Ala Ser Glu Asn Ala Ala Gln Val
625 630 635 640
Thr Ile Asp Glu Val Lys Gln Leu Pro Pro Leu Gln Glu Lys Glu Gln
645 650 655
Ser Arg Gly Asn Ser Gly Gly Gly Arg Phe Gly Arg Gly Gly Gly Gly
660 665 670
Arg Phe Ser Gly Gly Gly Arg Gly Gly Gly Phe Gly Gly Gly Gly Arg
675 680 685
Gly Arg Gly Gly Gly Gly Arg Gly Phe Ser Gly Arg Gly Gly Gly Gly
690 695 700
Asn Arg Phe Asn Arg Arg Asn
705 710
<210> 133
<211> 2016
<212> DNA
<213> Arabidopsis thaliana
<400> 133
atgccttccc taatgttatc tgataagaaa gaggagaaga agatgaagaa gaagatggct 60
ttggataccc cagagcttga ttctaagaag gggaagaagg agcagaagct gaaactatct 120
gattcggatg aagaagagtc ggagaagaag aagagtaaga agaaggataa gaagcgtaag 180
gcttccgagg aggaggatga agtgaagagc gattcaagct cggagaagaa gaagagcagc 240
aagaaggtta agttgggcgt tgaggatgtt gaagttgata accctaatgc tgtttctaag 300
tttcggattt ctgcgccttt gagggagaag cttaaggcga atggtattga agctcttttc 360
ccgattcagg cttccacgtt tgatatggtt ctcgatggtg ctgatttagt tggacgggct 420
cgtactggtc agggtaaaac attggctttt gtgttgccta tattggaatc tttggttaat 480
ggacctgcca aaagcaaaag gaagatggga tatggcaggt caccaagtgt tttggtcctt 540
ttaccgacca gagaattggc caagcaggtg gctgctgact ttgatgcata tggaggatca 600
cttgggttaa gttcatgttg tctctatgga ggtgatagct atccagttca ggagggtaaa 660
ttaaagagag gtgttgacat tgtagttgga acccctggtc gtattaagga tcatattgaa 720
agacaaaacc ttgatttcag ctatttacaa ttccgtgttc ttgatgaagc tgatgaaatg 780
ttgaggatgg gatttgttga ggatgttgaa cttattttag ggaaagtgga ggattctact 840
aaagtccaga ctcttctctt cagtgctact ttgccatcat gggtgaaaaa tatctctaac 900
aggtttctta aaagagacca gaagactatt gatcttgttg gtaatgataa aatgaaggcc 960
agtaatagtg ttcgacacat tgctattcct tgtaataagg cagccatggc tcggttgatt 1020
cctgatatta tcagttgcta tagcagtgga ggccaaacta ttattttcgc tgaaactaaa 1080
gttcaagttt ctgagctttc tggtttgttg gatggatcaa gagccttgca tggtgaaata 1140
cctcaatcac aacgtgaggt tactcttgct ggatttagga atggcaagtt tgcaacattg 1200
gtggctacaa atgttgctgc tcgtggtcta gatatcaatg atgtgcagtt aattatccag 1260
tgtgagcctc cacgtgaagt tgaagcatat attcatcgtt caggccgaac aggaagagct 1320
ggcaacacag gagttgcggt tacactctac gattctagaa agtcgagtgt atccaggatc 1380
gaaaaagaag ctggtatcaa atttgagcac cttgctgcac ctcaacctga tgaaattgcc 1440
agatctggtg gtatggaagc tgctgagaaa gttaaacaag tgtgtgacag tgtggttcct 1500
gcattccttg aagctgccaa ggaattatta gaaacttctg gtttatcagc tgaagtactc 1560
ctcgcaaaag ctcttgcaaa aactgcgggc ttcactgaga taaagaaaag gtcacttctt 1620
acatcaatgg agaactatgt gacactacat cttgaagcag ggaaaccaat atactcgcca 1680
tcatttgttt atggactgct aaggagagtt ttaccggatg acaaggtgga aatgattgaa 1740
gggttatctc taacagcaga taaaacagga gctgtgttcg atgttaagca atcagatcta 1800
gacttgttca ttgcaggagc acaaaagagt gctggaagta tgagtttgga agtggttaag 1860
gtgatgccta aactccagga gagagaacca ttgccacaaa aaagattcgg tggtggtggc 1920
agaggaaatc ggtttggtgg tggtggagga aatcggtttg gcggtggtgg tggcagagga 1980
agaggaggca gtggtggtag aggccagaga tattga 2016
<210> 134
<211> 671
<212> PRT
<213> Arabidopsis thaliana
<400> 134
Met Pro Ser Leu Met Leu Ser Asp Lys Lys Glu Glu Lys Lys Met Lys
1 5 10 15
Lys Lys Met Ala Leu Asp Thr Pro Glu Leu Asp Ser Lys Lys Gly Lys
20 25 30
Lys Glu Gln Lys Leu Lys Leu Ser Asp Ser Asp Glu Glu Glu Ser Glu
35 40 45
Lys Lys Lys Ser Lys Lys Lys Asp Lys Lys Arg Lys Ala Ser Glu Glu
50 55 60
Glu Asp Glu Val Lys Ser Asp Ser Ser Ser Glu Lys Lys Lys Ser Ser
65 70 75 80
Lys Lys Val Lys Leu Gly Val Glu Asp Val Glu Val Asp Asn Pro Asn
85 90 95
Ala Val Ser Lys Phe Arg Ile Ser Ala Pro Leu Arg Glu Lys Leu Lys
100 105 110
Ala Asn Gly Ile Glu Ala Leu Phe Pro Ile Gln Ala Ser Thr Phe Asp
115 120 125
Met Val Leu Asp Gly Ala Asp Leu Val Gly Arg Ala Arg Thr Gly Gln
130 135 140
Gly Lys Thr Leu Ala Phe Val Leu Pro Ile Leu Glu Ser Leu Val Asn
145 150 155 160
Gly Pro Ala Lys Ser Lys Arg Lys Met Gly Tyr Gly Arg Ser Pro Ser
165 170 175
Val Leu Val Leu Leu Pro Thr Arg Glu Leu Ala Lys Gln Val Ala Ala
180 185 190
Asp Phe Asp Ala Tyr Gly Gly Ser Leu Gly Leu Ser Ser Cys Cys Leu
195 200 205
Tyr Gly Gly Asp Ser Tyr Pro Val Gln Glu Gly Lys Leu Lys Arg Gly
210 215 220
Val Asp Ile Val Val Gly Thr Pro Gly Arg Ile Lys Asp His Ile Glu
225 230 235 240
Arg Gln Asn Leu Asp Phe Ser Tyr Leu Gln Phe Arg Val Leu Asp Glu
245 250 255
Ala Asp Glu Met Leu Arg Met Gly Phe Val Glu Asp Val Glu Leu Ile
260 265 270
Leu Gly Lys Val Glu Asp Ser Thr Lys Val Gln Thr Leu Leu Phe Ser
275 280 285
Ala Thr Leu Pro Ser Trp Val Lys Asn Ile Ser Asn Arg Phe Leu Lys
290 295 300
Arg Asp Gln Lys Thr Ile Asp Leu Val Gly Asn Asp Lys Met Lys Ala
305 310 315 320
Ser Asn Ser Val Arg His Ile Ala Ile Pro Cys Asn Lys Ala Ala Met
325 330 335
Ala Arg Leu Ile Pro Asp Ile Ile Ser Cys Tyr Ser Ser Gly Gly Gln
340 345 350
Thr Ile Ile Phe Ala Glu Thr Lys Val Gln Val Ser Glu Leu Ser Gly
355 360 365
Leu Leu Asp Gly Ser Arg Ala Leu His Gly Glu Ile Pro Gln Ser Gln
370 375 380
Arg Glu Val Thr Leu Ala Gly Phe Arg Asn Gly Lys Phe Ala Thr Leu
385 390 395 400
Val Ala Thr Asn Val Ala Ala Arg Gly Leu Asp Ile Asn Asp Val Gln
405 410 415
Leu Ile Ile Gln Cys Glu Pro Pro Arg Glu Val Glu Ala Tyr Ile His
420 425 430
Arg Ser Gly Arg Thr Gly Arg Ala Gly Asn Thr Gly Val Ala Val Thr
435 440 445
Leu Tyr Asp Ser Arg Lys Ser Ser Val Ser Arg Ile Glu Lys Glu Ala
450 455 460
Gly Ile Lys Phe Glu His Leu Ala Ala Pro Gln Pro Asp Glu Ile Ala
465 470 475 480
Arg Ser Gly Gly Met Glu Ala Ala Glu Lys Val Lys Gln Val Cys Asp
485 490 495
Ser Val Val Pro Ala Phe Leu Glu Ala Ala Lys Glu Leu Leu Glu Thr
500 505 510
Ser Gly Leu Ser Ala Glu Val Leu Leu Ala Lys Ala Leu Ala Lys Thr
515 520 525
Ala Gly Phe Thr Glu Ile Lys Lys Arg Ser Leu Leu Thr Ser Met Glu
530 535 540
Asn Tyr Val Thr Leu His Leu Glu Ala Gly Lys Pro Ile Tyr Ser Pro
545 550 555 560
Ser Phe Val Tyr Gly Leu Leu Arg Arg Val Leu Pro Asp Asp Lys Val
565 570 575
Glu Met Ile Glu Gly Leu Ser Leu Thr Ala Asp Lys Thr Gly Ala Val
580 585 590
Phe Asp Val Lys Gln Ser Asp Leu Asp Leu Phe Ile Ala Gly Ala Gln
595 600 605
Lys Ser Ala Gly Ser Met Ser Leu Glu Val Val Lys Val Met Pro Lys
610 615 620
Leu Gln Glu Arg Glu Pro Leu Pro Gln Lys Arg Phe Gly Gly Gly Gly
625 630 635 640
Arg Gly Asn Arg Phe Gly Gly Gly Gly Gly Asn Arg Phe Gly Gly Gly
645 650 655
Gly Gly Arg Gly Arg Gly Gly Ser Gly Gly Arg Gly Gln Arg Tyr
660 665 670
<210> 135
<211> 2226
<212> DNA
<213> Glycine max
<400> 135
aacgtttcca ccgcgaaaac cttggtcggc gcatactcct tattcttctc ctgcttctgc 60
acctcactgc tataccccct ttcaagcagg agaaaatctc tcactccttt ctctttctct 120
ccacgccgcc atcaccacac aaccatgcct tcgctctctc tctctgaccc tgacaccccc 180
aaacctattg ccaagaagaa aaccaaaacc cagtccctca ccgatcccga cctcgatggg 240
gtttcgggga agaagacgaa gaagcgcaag gcttcagatt tagaacaaga ggccatgccc 300
cccgccgctt acaacaacga tggggacgac gagaccagct ccgacctcgt ccaacccgaa 360
cctgcttcca gagaagacga caacaagaat aagaagaaaa agaagaaaaa ggttgttaag 420
tccgaagaaa aggaacagcc tttggtcacg gaggctaatg gagaaaagaa ggaggatccc 480
aacgcgcttt ccaacttcag gatttcggaa cccttgaggc aaaaattgaa ggagaagggt 540
atcgaatcac tgtttcccat tcaggccatg accttcgaca ccgttctcga tggttctgat 600
ttggtcggtc gggctcgcac cggtcagggt aaaactctgg catttgtgtt gcccatatta 660
gagtctttaa taaatggtcc aacaaaagct tcaagaaaga ctggcttcgg gaggactcca 720
agtgttcttg tgcttctacc tactagggaa ttggcctgtc aggtgcatgc tgattttgat 780
gtttatggtg gggcaatggg attgagttca tgttgtttat atggcggagc tccgtatcaa 840
ggtcaggaaa ttaagcttag gagaggtgtt gatattgtca ttggcacacc aggtcgtgtg 900
aaggatcata tcgagaaagg gaatattgac ctgagccaac taaagtttcg tgtccttgac 960
gaagccgatg aaatgctgag gatgggtttt gttgaagatg ttgaaatgat tctaggcaag 1020
gtagaaaatg ttaataaagt tcagacactt cttttcagtg ctactttgcc agactgggtt 1080
aagcaaattg ctgcaagatt tttgaagcca gataagaaaa ctgctgacct tgttggaaat 1140
acaaaaatga aggccagcat caatgttagg catattgttc ttccttgtac tagttctgcc 1200
agggcccaac ttatcccaga tattattcgc tgttatagca gtggaggccg gacaattgtt 1260
tttaccgaga caaaagaatc tgcttctcag cttgcaggga tcttgactgg agcaaaagct 1320
ctccatggtg acatacagca atcaacacgt gaggttacac tgtctggctt taggtctggg 1380
aaattcatga cattagttgc cacaaatgtg gcagctcgag gtcttgatat taatgatgtt 1440
cagttaatta tacagtgtga acccccacgg gatgtagaag cctatatcca tcgttctgga 1500
cgcacaggaa gagcaggtaa tactggggtt gctgttatgc tttatgaccc aaaaagatcg 1560
aacatatcta gaatagagag agagtctggt gtaaaatttg aacacgtatc tgctcctcag 1620
cctgatgata ttgccaaagc cgttagtggg gaagctgctg aaatgattat ccaagtgtct 1680
gatagtgtgg ttcctgcatt caagtctgct gctgaagatc ttttgaacaa ttctggttta 1740
ccagtcattg aattactggc aaaggctctt gcaaaggctg ttggttatac tgaagtaaag 1800
caaagatcac ttctaacttc catggagaac tatgttacat tacttcttga gactggcaaa 1860
ccaatctaca cccaatcttt tgcctatgga gtcttgagga gatttttgcc tgaagagaag 1920
gttgaggccg tgaaaggtct ttcaatcact gctgatggaa atggcgttgt ttttgatgta 1980
gcagctaaag atttagacat atatcttaac ggtcaggaaa atgcctcgaa tgtaagttta 2040
gagatagtga aaacattgcc acagttgcaa cagatggagc aacaccaaag aggtggcaga 2100
tttggtgatg gtggtggtcg tggttaccgg tttggtggaa gaggtggagg aggcaggaat 2160
ggtaggttct ccaatggtgg cggaagaggt ggacgtggcg gcaactgggg tggaaagaga 2220
tggtga 2226
<210> 136
<211> 741
<212> PRT
<213> Glycine max
<400> 136
Asn Val Ser Thr Ala Lys Thr Leu Val Gly Ala Tyr Ser Leu Phe Phe
1 5 10 15
Ser Cys Phe Cys Thr Ser Leu Leu Tyr Pro Leu Ser Ser Arg Arg Lys
20 25 30
Ser Leu Thr Pro Phe Ser Phe Ser Pro Arg Arg His His His Thr Thr
35 40 45
Met Pro Ser Leu Ser Leu Ser Asp Pro Asp Thr Pro Lys Pro Ile Ala
50 55 60
Lys Lys Lys Thr Lys Thr Gln Ser Leu Thr Asp Pro Asp Leu Asp Gly
65 70 75 80
Val Ser Gly Lys Lys Thr Lys Lys Arg Lys Ala Ser Asp Leu Glu Gln
85 90 95
Glu Ala Met Pro Pro Ala Ala Tyr Asn Asn Asp Gly Asp Asp Glu Thr
100 105 110
Ser Ser Asp Leu Val Gln Pro Glu Pro Ala Ser Arg Glu Asp Asp Asn
115 120 125
Lys Asn Lys Lys Lys Lys Lys Lys Lys Val Val Lys Ser Glu Glu Lys
130 135 140
Glu Gln Pro Leu Val Thr Glu Ala Asn Gly Glu Lys Lys Glu Asp Pro
145 150 155 160
Asn Ala Leu Ser Asn Phe Arg Ile Ser Glu Pro Leu Arg Gln Lys Leu
165 170 175
Lys Glu Lys Gly Ile Glu Ser Leu Phe Pro Ile Gln Ala Met Thr Phe
180 185 190
Asp Thr Val Leu Asp Gly Ser Asp Leu Val Gly Arg Ala Arg Thr Gly
195 200 205
Gln Gly Lys Thr Leu Ala Phe Val Leu Pro Ile Leu Glu Ser Leu Ile
210 215 220
Asn Gly Pro Thr Lys Ala Ser Arg Lys Thr Gly Phe Gly Arg Thr Pro
225 230 235 240
Ser Val Leu Val Leu Leu Pro Thr Arg Glu Leu Ala Cys Gln Val His
245 250 255
Ala Asp Phe Asp Val Tyr Gly Gly Ala Met Gly Leu Ser Ser Cys Cys
260 265 270
Leu Tyr Gly Gly Ala Pro Tyr Gln Gly Gln Glu Ile Lys Leu Arg Arg
275 280 285
Gly Val Asp Ile Val Ile Gly Thr Pro Gly Arg Val Lys Asp His Ile
290 295 300
Glu Lys Gly Asn Ile Asp Leu Ser Gln Leu Lys Phe Arg Val Leu Asp
305 310 315 320
Glu Ala Asp Glu Met Leu Arg Met Gly Phe Val Glu Asp Val Glu Met
325 330 335
Ile Leu Gly Lys Val Glu Asn Val Asn Lys Val Gln Thr Leu Leu Phe
340 345 350
Ser Ala Thr Leu Pro Asp Trp Val Lys Gln Ile Ala Ala Arg Phe Leu
355 360 365
Lys Pro Asp Lys Lys Thr Ala Asp Leu Val Gly Asn Thr Lys Met Lys
370 375 380
Ala Ser Ile Asn Val Arg His Ile Val Leu Pro Cys Thr Ser Ser Ala
385 390 395 400
Arg Ala Gln Leu Ile Pro Asp Ile Ile Arg Cys Tyr Ser Ser Gly Gly
405 410 415
Arg Thr Ile Val Phe Thr Glu Thr Lys Glu Ser Ala Ser Gln Leu Ala
420 425 430
Gly Ile Leu Thr Gly Ala Lys Ala Leu His Gly Asp Ile Gln Gln Ser
435 440 445
Thr Arg Glu Val Thr Leu Ser Gly Phe Arg Ser Gly Lys Phe Met Thr
450 455 460
Leu Val Ala Thr Asn Val Ala Ala Arg Gly Leu Asp Ile Asn Asp Val
465 470 475 480
Gln Leu Ile Ile Gln Cys Glu Pro Pro Arg Asp Val Glu Ala Tyr Ile
485 490 495
His Arg Ser Gly Arg Thr Gly Arg Ala Gly Asn Thr Gly Val Ala Val
500 505 510
Met Leu Tyr Asp Pro Lys Arg Ser Asn Ile Ser Arg Ile Glu Arg Glu
515 520 525
Ser Gly Val Lys Phe Glu His Val Ser Ala Pro Gln Pro Asp Asp Ile
530 535 540
Ala Lys Ala Val Ser Gly Glu Ala Ala Glu Met Ile Ile Gln Val Ser
545 550 555 560
Asp Ser Val Val Pro Ala Phe Lys Ser Ala Ala Glu Asp Leu Leu Asn
565 570 575
Asn Ser Gly Leu Pro Val Ile Glu Leu Leu Ala Lys Ala Leu Ala Lys
580 585 590
Ala Val Gly Tyr Thr Glu Val Lys Gln Arg Ser Leu Leu Thr Ser Met
595 600 605
Glu Asn Tyr Val Thr Leu Leu Leu Glu Thr Gly Lys Pro Ile Tyr Thr
610 615 620
Gln Ser Phe Ala Tyr Gly Val Leu Arg Arg Phe Leu Pro Glu Glu Lys
625 630 635 640
Val Glu Ala Val Lys Gly Leu Ser Ile Thr Ala Asp Gly Asn Gly Val
645 650 655
Val Phe Asp Val Ala Ala Lys Asp Leu Asp Ile Tyr Leu Asn Gly Gln
660 665 670
Glu Asn Ala Ser Asn Val Ser Leu Glu Ile Val Lys Thr Leu Pro Gln
675 680 685
Leu Gln Gln Met Glu Gln His Gln Arg Gly Gly Arg Phe Gly Asp Gly
690 695 700
Gly Gly Arg Gly Tyr Arg Phe Gly Gly Arg Gly Gly Gly Gly Arg Asn
705 710 715 720
Gly Arg Phe Ser Asn Gly Gly Gly Arg Gly Gly Arg Gly Gly Asn Trp
725 730 735
Gly Gly Lys Arg Trp
740
<210> 137
<211> 522
<212> DNA
<213> Oryza sativa
<400> 137
atgccgaaga tggtggtgga ggtggccgac cggtacacgg cgaggaggga cgggcggccg 60
aacaactccg gcgaggcgga cggagaggtt cgcggcgacg ttccggcgaa accgagggca 120
ggccggggtg gagggcgatg cggcgtcgcc gagggaggag acggcgacgt cggccgacgc 180
tctggcgcgg cgaccggggc agccggagga gatgtcgacg aggaggaagg tgaggccggc 240
gcgggcgacg gcgttccggc gaaattcggg cgaggaggag gtgatgccgg ggaagaaggc 300
gatgccgcgg tgtcgaagga ggagacggcg gcgtcgatcg gcgcaccggc gatgaacagc 360
agccggctgg agggagtaca gtggcaccaa tgccactggt tgccggcggg ggcaccgttc 420
cggcgagttt ccggcgagga gacgctttat attgccctct catgtgttac gtcacctaaa 480
ggactccgtg tcctgatcga gaacaaccca cctggatatt aa 522
<210> 138
<211> 173
<212> PRT
<213> Oryza sativa
<400> 138
Met Pro Lys Met Val Val Glu Val Ala Asp Arg Tyr Thr Ala Arg Arg
1 5 10 15
Asp Gly Arg Pro Asn Asn Ser Gly Glu Ala Asp Gly Glu Val Arg Gly
20 25 30
Asp Val Pro Ala Lys Pro Arg Ala Gly Arg Gly Gly Gly Arg Cys Gly
35 40 45
Val Ala Glu Gly Gly Asp Gly Asp Val Gly Arg Arg Ser Gly Ala Ala
50 55 60
Thr Gly Ala Ala Gly Gly Asp Val Asp Glu Glu Glu Gly Glu Ala Gly
65 70 75 80
Ala Gly Asp Gly Val Pro Ala Lys Phe Gly Arg Gly Gly Gly Asp Ala
85 90 95
Gly Glu Glu Gly Asp Ala Ala Val Ser Lys Glu Glu Thr Ala Ala Ser
100 105 110
Ile Gly Ala Pro Ala Met Asn Ser Ser Arg Leu Glu Gly Val Gln Trp
115 120 125
His Gln Cys His Trp Leu Pro Ala Gly Ala Pro Phe Arg Arg Val Ser
130 135 140
Gly Glu Glu Thr Leu Tyr Ile Ala Leu Ser Cys Val Thr Ser Pro Lys
145 150 155 160
Gly Leu Arg Val Leu Ile Glu Asn Asn Pro Pro Gly Tyr
165 170
<210> 139
<211> 423
<212> DNA
<213> Zea mays
<400> 139
atgcagatcc gggtgcagtg cgcgtgcggc gagtcgtcgt gcccggaatg ggccgtcgtg 60
gagctgcagg gcgtggtgca gccgcaggcc tccttcgccg gcgacatccg gggactccac 120
atcggccgcc tctgctcctc cccttcccca gcctccgcct cgtccaaggc agggttcacc 180
ttcacggtgg ggtaccacga gctcgccggc acaaaggtga cgctcaagaa gcccctgctg 240
gtgctcagga agaagaaggt taatgctggt gctcaggaag aagagccacc gacggcggcg 300
gaggtggagc tggaggtgat cggcatcatc cggcacaaga tcctattcaa ggaccgcccc 360
aaggccctca tctcaaagcc gccaaccaag gagaagaagg ccgtgcagcc agcagcaaag 420
tga 423
<210> 140
<211> 140
<212> PRT
<213> Zea mays
<400> 140
Met Gln Ile Arg Val Gln Cys Ala Cys Gly Glu Ser Ser Cys Pro Glu
1 5 10 15
Trp Ala Val Val Glu Leu Gln Gly Val Val Gln Pro Gln Ala Ser Phe
20 25 30
Ala Gly Asp Ile Arg Gly Leu His Ile Gly Arg Leu Cys Ser Ser Pro
35 40 45
Ser Pro Ala Ser Ala Ser Ser Lys Ala Gly Phe Thr Phe Thr Val Gly
50 55 60
Tyr His Glu Leu Ala Gly Thr Lys Val Thr Leu Lys Lys Pro Leu Leu
65 70 75 80
Val Leu Arg Lys Lys Lys Val Asn Ala Gly Ala Gln Glu Glu Glu Pro
85 90 95
Pro Thr Ala Ala Glu Val Glu Leu Glu Val Ile Gly Ile Ile Arg His
100 105 110
Lys Ile Leu Phe Lys Asp Arg Pro Lys Ala Leu Ile Ser Lys Pro Pro
115 120 125
Thr Lys Glu Lys Lys Ala Val Gln Pro Ala Ala Lys
130 135 140
<210> 141
<211> 426
<212> DNA
<213> Sorghum bicolor
<400> 141
atgcagatcc gcgtgcagtg cgggtgcggc gagtcgtcgt gcccggagtg ggccgtcgtg 60
gagctgcagg gcgtggtgca gccgcaggcc tccttcgccg gcgacatccg cggactccac 120
atcggccgcc tctgctccgc cccctccccg tcctcatctt ccaagcaggc agggtacacc 180
ttcacggttg ggtaccacga gctcgccggg accaaggtga cgctcaagaa gcccctgctg 240
gtgctccgga agaagaaggt gaatgctggt tgtggggaac aagagccacc ggcggcggcg 300
gaggaggtgg agctggaggt gattggcatc atccggcaca agatcctctt caaggaccgt 360
cccaaggccc tcatctcaaa gccgccaacc aaggagaaga agaccgtgca gccagcagca 420
aagtga 426
<210> 142
<211> 141
<212> PRT
<213> Sorghum bicolor
<400> 142
Met Gln Ile Arg Val Gln Cys Gly Cys Gly Glu Ser Ser Cys Pro Glu
1 5 10 15
Trp Ala Val Val Glu Leu Gln Gly Val Val Gln Pro Gln Ala Ser Phe
20 25 30
Ala Gly Asp Ile Arg Gly Leu His Ile Gly Arg Leu Cys Ser Ala Pro
35 40 45
Ser Pro Ser Ser Ser Ser Lys Gln Ala Gly Tyr Thr Phe Thr Val Gly
50 55 60
Tyr His Glu Leu Ala Gly Thr Lys Val Thr Leu Lys Lys Pro Leu Leu
65 70 75 80
Val Leu Arg Lys Lys Lys Val Asn Ala Gly Cys Gly Glu Gln Glu Pro
85 90 95
Pro Ala Ala Ala Glu Glu Val Glu Leu Glu Val Ile Gly Ile Ile Arg
100 105 110
His Lys Ile Leu Phe Lys Asp Arg Pro Lys Ala Leu Ile Ser Lys Pro
115 120 125
Pro Thr Lys Glu Lys Lys Thr Val Gln Pro Ala Ala Lys
130 135 140
<210> 143
<211> 438
<212> DNA
<213> Arabidopsis thaliana
<400> 143
atggagattc gagtgaagtg tagatgtgga gaagaagagt gtagcgaatg ggcgatcgta 60
gagcttcaag gagtcgtcga gactcaagct tccttccaag gttctattca gaatctcgag 120
atcggtcgtc tctgccactc cgattcctca cagggaacat acacgttcac ggtgggttac 180
catgaactcg taggctctaa agtgactcta aagaagccat tactggtttt gaagaagctt 240
caatttgatg aagtttcagg gaaagcaacg gagcttgaag ttgttgggat tatacgaaca 300
aagatcttgt ttaaaaccag acctaagcct cttatttccg gtaacaacaa cctctctctg 360
aacaaattga aacctctcta ttgtttcatt atctctgctt gtttcaataa tgtagtctct 420
gtttgtacta ttgaatga 438
<210> 144
<211> 145
<212> PRT
<213> Arabidopsis thaliana
<400> 144
Met Glu Ile Arg Val Lys Cys Arg Cys Gly Glu Glu Glu Cys Ser Glu
1 5 10 15
Trp Ala Ile Val Glu Leu Gln Gly Val Val Glu Thr Gln Ala Ser Phe
20 25 30
Gln Gly Ser Ile Gln Asn Leu Glu Ile Gly Arg Leu Cys His Ser Asp
35 40 45
Ser Ser Gln Gly Thr Tyr Thr Phe Thr Val Gly Tyr His Glu Leu Val
50 55 60
Gly Ser Lys Val Thr Leu Lys Lys Pro Leu Leu Val Leu Lys Lys Leu
65 70 75 80
Gln Phe Asp Glu Val Ser Gly Lys Ala Thr Glu Leu Glu Val Val Gly
85 90 95
Ile Ile Arg Thr Lys Ile Leu Phe Lys Thr Arg Pro Lys Pro Leu Ile
100 105 110
Ser Gly Asn Asn Asn Leu Ser Leu Asn Lys Leu Lys Pro Leu Tyr Cys
115 120 125
Phe Ile Ile Ser Ala Cys Phe Asn Asn Val Val Ser Val Cys Thr Ile
130 135 140
Glu
145
<210> 145
<211> 414
<212> DNA
<213> Glycine max
<400> 145
atgcagattc gagttcgatg caactgtgga gaaggaagct gcgaggaatg gggggttata 60
gaacttcaag gagtggtgga gccacagcct ggattccacg attccctcca aaatcttcac 120
atcggcactt tgtgtcgtcc ttcttctcag gaagtctaca ccttcactgt tggataccat 180
gaactgacgg ggtcaaaggt tcccttaaag aagccaatgg tggtgctcaa gaaagtaaag 240
catcctgatg gagaaagtgg ttgtaaggtg gagctgcaag ttgttggagt cattagacat 300
aagattctgt tcaagaacag accaaaggct ctcatttcta agccacagat aacatccagg 360
gaaagacaaa agcctatcat gttagggtct tctccttcaa atcaaactgc ttaa 414
<210> 146
<211> 137
<212> PRT
<213> Glycine max
<400> 146
Met Gln Ile Arg Val Arg Cys Asn Cys Gly Glu Gly Ser Cys Glu Glu
1 5 10 15
Trp Gly Val Ile Glu Leu Gln Gly Val Val Glu Pro Gln Pro Gly Phe
20 25 30
His Asp Ser Leu Gln Asn Leu His Ile Gly Thr Leu Cys Arg Pro Ser
35 40 45
Ser Gln Glu Val Tyr Thr Phe Thr Val Gly Tyr His Glu Leu Thr Gly
50 55 60
Ser Lys Val Pro Leu Lys Lys Pro Met Val Val Leu Lys Lys Val Lys
65 70 75 80
His Pro Asp Gly Glu Ser Gly Cys Lys Val Glu Leu Gln Val Val Gly
85 90 95
Val Ile Arg His Lys Ile Leu Phe Lys Asn Arg Pro Lys Ala Leu Ile
100 105 110
Ser Lys Pro Gln Ile Thr Ser Arg Glu Arg Gln Lys Pro Ile Met Leu
115 120 125
Gly Ser Ser Pro Ser Asn Gln Thr Ala
130 135
<210> 147
<211> 1182
<212> DNA
<213> Oryza sativa
<400> 147
atggctgtgc ggctctgctg ctgctgcttc ctcctcttcg tcaccatcgc tgccgccggt 60
ggctccctga cgcggacgaa cgtcgcgacg ctgccggggt tcgacggcgc gctgccttca 120
cgcctcgaga ccgggtacgt gacggtcgac gaggagaacg gcgccgagct gttctactac 180
ttcatcgagt cggagggcga ccccagcacc gaccccgtcc tcctctggat caccggcggc 240
gaccgctgct ccgtcctcag cgccctcttc ttcgagattg ggccgttgaa gctggtcatc 300
gagccctaca acggcagctt gccgcggctg cactaccacc cttactcgtg gacaaaggtc 360
gccagcatcc tcttcgtcga ctcgccggtc ggcgccggct tctccttctc aagggaccct 420
aaggggtacg acgtcggcga cgtctctgct tcgatgcagc tcatcaagct tctcagggag 480
tggttcactg agtatccgca ctacctctca aatccgttct acgttggagg agactcctat 540
gccggaaaga tcgtcccgtt tatcgtgcag aagatctcgg aagatattga agctggcgtc 600
agaccgacgt ttaatctgaa gggctatctg gtaggtaatc cgagtacagg ggaacgcatt 660
gacctcgaat caagagtgcc gtacagccat ggagtcggaa tcatttcaga tcagttatat 720
gagatgataa tggagcactg cgaaggtgag gattatgaca atcccagcaa cgtgatttgc 780
caacaagctt tggctaggtt tgacagccta ctacacgaag gttcgagggc ccaaattttg 840
aacccaaatt gcatttatgt gtcccccaag ccaaatcatg agacaatcga cagaaagatc 900
ttgaaggggg agcatggagg actaaaacat ccacctcctc aaccttcaat aaaatgtggt 960
gtctatgcca actatttgtc atatttttgg gcaaacaaca actttacacg aagaactcta 1020
ggaatcaaga agggcaccat aaatgaatgg gttaggtgcc acgaacatga tctaccatac 1080
aacatcgaca tcaggagcag cataaagtat catcgaaatg ttacactaaa agtggtgacc 1140
acgatgctgt tgtgccattc ctcgggacac aagcctgggt ga 1182
<210> 148
<211> 393
<212> PRT
<213> Oryza sativa
<400> 148
Met Ala Val Arg Leu Cys Cys Cys Cys Phe Leu Leu Phe Val Thr Ile
1 5 10 15
Ala Ala Ala Gly Gly Ser Leu Thr Arg Thr Asn Val Ala Thr Leu Pro
20 25 30
Gly Phe Asp Gly Ala Leu Pro Ser Arg Leu Glu Thr Gly Tyr Val Thr
35 40 45
Val Asp Glu Glu Asn Gly Ala Glu Leu Phe Tyr Tyr Phe Ile Glu Ser
50 55 60
Glu Gly Asp Pro Ser Thr Asp Pro Val Leu Leu Trp Ile Thr Gly Gly
65 70 75 80
Asp Arg Cys Ser Val Leu Ser Ala Leu Phe Phe Glu Ile Gly Pro Leu
85 90 95
Lys Leu Val Ile Glu Pro Tyr Asn Gly Ser Leu Pro Arg Leu His Tyr
100 105 110
His Pro Tyr Ser Trp Thr Lys Val Ala Ser Ile Leu Phe Val Asp Ser
115 120 125
Pro Val Gly Ala Gly Phe Ser Phe Ser Arg Asp Pro Lys Gly Tyr Asp
130 135 140
Val Gly Asp Val Ser Ala Ser Met Gln Leu Ile Lys Leu Leu Arg Glu
145 150 155 160
Trp Phe Thr Glu Tyr Pro His Tyr Leu Ser Asn Pro Phe Tyr Val Gly
165 170 175
Gly Asp Ser Tyr Ala Gly Lys Ile Val Pro Phe Ile Val Gln Lys Ile
180 185 190
Ser Glu Asp Ile Glu Ala Gly Val Arg Pro Thr Phe Asn Leu Lys Gly
195 200 205
Tyr Leu Val Gly Asn Pro Ser Thr Gly Glu Arg Ile Asp Leu Glu Ser
210 215 220
Arg Val Pro Tyr Ser His Gly Val Gly Ile Ile Ser Asp Gln Leu Tyr
225 230 235 240
Glu Met Ile Met Glu His Cys Glu Gly Glu Asp Tyr Asp Asn Pro Ser
245 250 255
Asn Val Ile Cys Gln Gln Ala Leu Ala Arg Phe Asp Ser Leu Leu His
260 265 270
Glu Gly Ser Arg Ala Gln Ile Leu Asn Pro Asn Cys Ile Tyr Val Ser
275 280 285
Pro Lys Pro Asn His Glu Thr Ile Asp Arg Lys Ile Leu Lys Gly Glu
290 295 300
His Gly Gly Leu Lys His Pro Pro Pro Gln Pro Ser Ile Lys Cys Gly
305 310 315 320
Val Tyr Ala Asn Tyr Leu Ser Tyr Phe Trp Ala Asn Asn Asn Phe Thr
325 330 335
Arg Arg Thr Leu Gly Ile Lys Lys Gly Thr Ile Asn Glu Trp Val Arg
340 345 350
Cys His Glu His Asp Leu Pro Tyr Asn Ile Asp Ile Arg Ser Ser Ile
355 360 365
Lys Tyr His Arg Asn Val Thr Leu Lys Val Val Thr Thr Met Leu Leu
370 375 380
Cys His Ser Ser Gly His Lys Pro Gly
385 390
<210> 149
<211> 1476
<212> DNA
<213> Zea mays
<400> 149
atgacgctgg tgaggatgcc ttgccgcctc tgctgcttcc tgctcgtcgt cgccgcctcc 60
gccgtctccg ggcgggggcg ggtggtcacc accctcccgg gctacgaggg ccgcctcccc 120
ttccacctcg aaacagggta tgtggaggtg gacgaggacg ccggcgcgga gctcttctac 180
tacttcgtcc gggccgagtc cggcgccgac gacagcgaca ccccgttcgt cctccggatc 240
cccggcggcc aacgctgctc cgccttcagc ggcctcgcct acgagatagg tcccatcatg 300
ttcgtcgtgg agccctacaa cggcagcttg ccgcgcctgc gctacaaccc aaactcgtgg 360
acaaaggtgg cgcatattct ttttgtggat tcgccagttg gggccgggtt ttccttctcc 420
agagatgcca aaggctacaa cgctggagcg gtatcaacca cgctgcactt ggcgaagttc 480
ctcaacaagt ggttcaacga ccatcctgag taccatgcaa atcctttcta catcgatgga 540
gaatcatacg ctggaaaaat tgtaccattc cttgcacaga tgatttcaga aggtattgga 600
gcaggaatga agtcggcacc tcgtctcaag ggctatctag tgggcaaccc gtccacagag 660
gagcgtattg atgttagcgc tagagtgcct tgtgctcacg ggtttgggat tatatcacat 720
cagttgtatg agatgatatt ggggcattgc catggagagg actactccaa tcctgcaaaa 780
gagttgtgtg gtcaggcact gaagaccttc aatgatctca cctcggaagt tgcgcaaggc 840
catgtactgc aggagaaatg cgtcgcggcg tcgtcgtctc ctgtgctgaa cgcaaacagc 900
agggtggccg gcggctcgtc gtcgtgggcc tcagatggca ggaagatcct acgggaggaa 960
gaaatggtgg gacgacgcgg agtactgggc aagctatttc atccaccggc tcttcctcca 1020
ttcagttgca gagtttaccg gtactacctg tcgtatttct gggcaaacga caggcgcacc 1080
cgggatgctc ttgggatcaa ggagggcacc gtggatgagt gggtcagatg ccacaacgac 1140
gaccaagagt tgccttacga gagcgagctg aagagtgtcg tcaagtacca ccggaacctg 1200
acgtccagag gctaccgagc catggtatac agcggcgacc atgacctgct ggtgccacat 1260
ctgggcaccc aggcctgggt caggtcgctc aacttccccg tcgtggacga ctggagggcg 1320
tggcatctcg gtggccagtc agctggattt acaataactt actcaaacaa catgacattc 1380
gcgacgatca agggcgccgg gcatacagca cctgagtacg agccggagag gtgtttcgcc 1440
atgttcagcc gttggatcct caaccggcca ctctaa 1476
<210> 150
<211> 491
<212> PRT
<213> Zea mays
<400> 150
Met Thr Leu Val Arg Met Pro Cys Arg Leu Cys Cys Phe Leu Leu Val
1 5 10 15
Val Ala Ala Ser Ala Val Ser Gly Arg Gly Arg Val Val Thr Thr Leu
20 25 30
Pro Gly Tyr Glu Gly Arg Leu Pro Phe His Leu Glu Thr Gly Tyr Val
35 40 45
Glu Val Asp Glu Asp Ala Gly Ala Glu Leu Phe Tyr Tyr Phe Val Arg
50 55 60
Ala Glu Ser Gly Ala Asp Asp Ser Asp Thr Pro Phe Val Leu Arg Ile
65 70 75 80
Pro Gly Gly Gln Arg Cys Ser Ala Phe Ser Gly Leu Ala Tyr Glu Ile
85 90 95
Gly Pro Ile Met Phe Val Val Glu Pro Tyr Asn Gly Ser Leu Pro Arg
100 105 110
Leu Arg Tyr Asn Pro Asn Ser Trp Thr Lys Val Ala His Ile Leu Phe
115 120 125
Val Asp Ser Pro Val Gly Ala Gly Phe Ser Phe Ser Arg Asp Ala Lys
130 135 140
Gly Tyr Asn Ala Gly Ala Val Ser Thr Thr Leu His Leu Ala Lys Phe
145 150 155 160
Leu Asn Lys Trp Phe Asn Asp His Pro Glu Tyr His Ala Asn Pro Phe
165 170 175
Tyr Ile Asp Gly Glu Ser Tyr Ala Gly Lys Ile Val Pro Phe Leu Ala
180 185 190
Gln Met Ile Ser Glu Gly Ile Gly Ala Gly Met Lys Ser Ala Pro Arg
195 200 205
Leu Lys Gly Tyr Leu Val Gly Asn Pro Ser Thr Glu Glu Arg Ile Asp
210 215 220
Val Ser Ala Arg Val Pro Cys Ala His Gly Phe Gly Ile Ile Ser His
225 230 235 240
Gln Leu Tyr Glu Met Ile Leu Gly His Cys His Gly Glu Asp Tyr Ser
245 250 255
Asn Pro Ala Lys Glu Leu Cys Gly Gln Ala Leu Lys Thr Phe Asn Asp
260 265 270
Leu Thr Ser Glu Val Ala Gln Gly His Val Leu Gln Glu Lys Cys Val
275 280 285
Ala Ala Ser Ser Ser Pro Val Leu Asn Ala Asn Ser Arg Val Ala Gly
290 295 300
Gly Ser Ser Ser Trp Ala Ser Asp Gly Arg Lys Ile Leu Arg Glu Glu
305 310 315 320
Glu Met Val Gly Arg Arg Gly Val Leu Gly Lys Leu Phe His Pro Pro
325 330 335
Ala Leu Pro Pro Phe Ser Cys Arg Val Tyr Arg Tyr Tyr Leu Ser Tyr
340 345 350
Phe Trp Ala Asn Asp Arg Arg Thr Arg Asp Ala Leu Gly Ile Lys Glu
355 360 365
Gly Thr Val Asp Glu Trp Val Arg Cys His Asn Asp Asp Gln Glu Leu
370 375 380
Pro Tyr Glu Ser Glu Leu Lys Ser Val Val Lys Tyr His Arg Asn Leu
385 390 395 400
Thr Ser Arg Gly Tyr Arg Ala Met Val Tyr Ser Gly Asp His Asp Leu
405 410 415
Leu Val Pro His Leu Gly Thr Gln Ala Trp Val Arg Ser Leu Asn Phe
420 425 430
Pro Val Val Asp Asp Trp Arg Ala Trp His Leu Gly Gly Gln Ser Ala
435 440 445
Gly Phe Thr Ile Thr Tyr Ser Asn Asn Met Thr Phe Ala Thr Ile Lys
450 455 460
Gly Ala Gly His Thr Ala Pro Glu Tyr Glu Pro Glu Arg Cys Phe Ala
465 470 475 480
Met Phe Ser Arg Trp Ile Leu Asn Arg Pro Leu
485 490
<210> 151
<211> 1263
<212> DNA
<213> Sorghum bicolor
<400> 151
atgaggacga tgatgccttg ccgcctcagc tgcttcctgc tcgtcgtcgc cgccgcctcc 60
ggctctgctg gctccgggca ggggcgggtg gtcaccaccc tcccgggctt cgagggccgc 120
ctccccttcc acctcgaaac agggtacgtg gaggtggacg aggacgccgg cgcggagctc 180
ttctactact tcgtccagtc cgagtccgag tccgccggcg acgcccctct cctcctctgg 240
ctcaccggcg gccaacgctg ctccgccttg agcggcctcg cctacgagat tggtcccatc 300
aggttcgtcg tggagcccta cgacggcacc ttaccgcgcc tgcgctacga ctcgagaaac 360
tcgtggacaa aggtggcaca tattcttttt gttgattcgc cagttggggc tgggttttcc 420
ttctccaaag atcccaaagg ctactatgtt ggagacatat catcctccat gcagttgcat 480
aagttcctca acaagtggtt caacgagcat ccggactacc ttgcaaatcc tttctacatc 540
ggtggagaat catacgctgg aaaaactgta ccattccttg cacagatgat ttcagaaggt 600
gttgaagcag gaatgaagtc cgaacctaat ctcaagggct atctagtggg caacccgtcc 660
acagaggaac gtattgattt tggctctaga gtgcctcatg ctcacggctt tggaattata 720
tcacatcagc tgtatgagac gatatccggg cattgccaag gagaagacta ctccaatcct 780
gcaaatgagt tgtgtggtca ggctctgaat accttcaatg atagttactc ctactccctg 840
tcgtatttct gggcaaacga cagacgcacc cgagatgctc ttgggatcaa ggagggtacc 900
gtggatgagt gggtcagatg cgacgatgaa gcagagctgc cttacgagcg cgacctcaag 960
agtgtcgtca agtaccactg gaacctgacc tccagaggct accgcgccct cgtattcagc 1020
ggcgaccatg acctgatggt gccacacctg ggcacccagg cctgggtcag atctctcaac 1080
ttccccatcg tcgacgactg gagggcctgg catcttggtg ggcagtcagc tggattcaca 1140
atcagttact caaacaacat gacattcgcg acgatcaagg gcggcggaca tacagcacct 1200
gagtacgagc cggagaggtg tttcgccatg ttcagccgct gggtgctcaa ccggccactc 1260
taa 1263
<210> 152
<211> 420
<212> PRT
<213> Sorghum bicolor
<400> 152
Met Arg Thr Met Met Pro Cys Arg Leu Ser Cys Phe Leu Leu Val Val
1 5 10 15
Ala Ala Ala Ser Gly Ser Ala Gly Ser Gly Gln Gly Arg Val Val Thr
20 25 30
Thr Leu Pro Gly Phe Glu Gly Arg Leu Pro Phe His Leu Glu Thr Gly
35 40 45
Tyr Val Glu Val Asp Glu Asp Ala Gly Ala Glu Leu Phe Tyr Tyr Phe
50 55 60
Val Gln Ser Glu Ser Glu Ser Ala Gly Asp Ala Pro Leu Leu Leu Trp
65 70 75 80
Leu Thr Gly Gly Gln Arg Cys Ser Ala Leu Ser Gly Leu Ala Tyr Glu
85 90 95
Ile Gly Pro Ile Arg Phe Val Val Glu Pro Tyr Asp Gly Thr Leu Pro
100 105 110
Arg Leu Arg Tyr Asp Ser Arg Asn Ser Trp Thr Lys Val Ala His Ile
115 120 125
Leu Phe Val Asp Ser Pro Val Gly Ala Gly Phe Ser Phe Ser Lys Asp
130 135 140
Pro Lys Gly Tyr Tyr Val Gly Asp Ile Ser Ser Ser Met Gln Leu His
145 150 155 160
Lys Phe Leu Asn Lys Trp Phe Asn Glu His Pro Asp Tyr Leu Ala Asn
165 170 175
Pro Phe Tyr Ile Gly Gly Glu Ser Tyr Ala Gly Lys Thr Val Pro Phe
180 185 190
Leu Ala Gln Met Ile Ser Glu Gly Val Glu Ala Gly Met Lys Ser Glu
195 200 205
Pro Asn Leu Lys Gly Tyr Leu Val Gly Asn Pro Ser Thr Glu Glu Arg
210 215 220
Ile Asp Phe Gly Ser Arg Val Pro His Ala His Gly Phe Gly Ile Ile
225 230 235 240
Ser His Gln Leu Tyr Glu Thr Ile Ser Gly His Cys Gln Gly Glu Asp
245 250 255
Tyr Ser Asn Pro Ala Asn Glu Leu Cys Gly Gln Ala Leu Asn Thr Phe
260 265 270
Asn Asp Ser Tyr Ser Tyr Ser Leu Ser Tyr Phe Trp Ala Asn Asp Arg
275 280 285
Arg Thr Arg Asp Ala Leu Gly Ile Lys Glu Gly Thr Val Asp Glu Trp
290 295 300
Val Arg Cys Asp Asp Glu Ala Glu Leu Pro Tyr Glu Arg Asp Leu Lys
305 310 315 320
Ser Val Val Lys Tyr His Trp Asn Leu Thr Ser Arg Gly Tyr Arg Ala
325 330 335
Leu Val Phe Ser Gly Asp His Asp Leu Met Val Pro His Leu Gly Thr
340 345 350
Gln Ala Trp Val Arg Ser Leu Asn Phe Pro Ile Val Asp Asp Trp Arg
355 360 365
Ala Trp His Leu Gly Gly Gln Ser Ala Gly Phe Thr Ile Ser Tyr Ser
370 375 380
Asn Asn Met Thr Phe Ala Thr Ile Lys Gly Gly Gly His Thr Ala Pro
385 390 395 400
Glu Tyr Glu Pro Glu Arg Cys Phe Ala Met Phe Ser Arg Trp Val Leu
405 410 415
Asn Arg Pro Leu
420
<210> 153
<211> 1314
<212> DNA
<213> Arabidopsis thaliana
<400> 153
atggggaaag agtgctacta cttgtcatgg atactcaagt ttcatcttct acttgttttg 60
attcagcttg ttgattcagg atctaccatc agatttcttc ctggtttcca aggccctctt 120
cctttcgagc tcgagaccgg gtacattggt gtgggtgagg cagagaaaga tcaaatgttc 180
tactacttca tcaaatctga gagtaatcca gagaaagacc ctcttcttct ctggttaagt 240
ggaggtcctt tctgttcttc gttcactgct cttatttatg agaatgggcc aattgctttc 300
aaggctgagg aatacaatgg aagtatcccc tctttggtct ctaccacata tgcatggact 360
aaggtggcga gcattctcta tttggatcag cctgttggga ctggcttctc ctactccaga 420
aatccacttg ctgatatacc aagtgacaca ggggtagcta agccggtcaa cgagtttctt 480
cataagtggc tagacaaaca tcctgagttc ttatccaatc cgctctatgt cgccggaaat 540
tcttattccg gtattgtcat tccgaccatc gttcaagaaa tctcaaatgg aaaccattta 600
gacagcaaac ctcaaataaa tcttcagggc tttgtactcg gaaacccggc aacagacact 660
gatattgatc ttaactctcg cattccattt gctcatggaa aggcactgat ctctgatgaa 720
cactatgagt ctctaaagag aagttgtcaa gggaattata ttagcgtgaa tcctcgtaac 780
acaaaatgct tgaaactcct tgaagacttt aaaaagtgtg tttctggaat aagtgaagaa 840
tatattctaa aacctgactg catgtggctt tattcttgta tggcaaatct gcattcgcta 900
tctgaatatt gggcaaatga gaaaagcgtg cgcaaagctc ttctagtgaa cgagggtacc 960
gtaagaaaat ggatacgatg taatacggaa atcgcataca ataaggacat taggagtagt 1020
gtgccatatc ataagtatat tagcattgaa gggtatcgat ctctcgtctt cagtggtgat 1080
cacgacatgt tagtaccttt ccttggaact caagcatgga taagatctct caactattcc 1140
attgttgacg attggagacc atggatggtt cagaatcaag ttgctggata cacaaggact 1200
tatgcgaata agatgacatt tgccactgtc aaaggaggcg ggcacacgag tgagtataaa 1260
ccagtggaga cctatatcat gatcaagagg tggttaagtg gtcaacctct ataa 1314
<210> 154
<211> 437
<212> PRT
<213> Arabidopsis thaliana
<400> 154
Met Gly Lys Glu Cys Tyr Tyr Leu Ser Trp Ile Leu Lys Phe His Leu
1 5 10 15
Leu Leu Val Leu Ile Gln Leu Val Asp Ser Gly Ser Thr Ile Arg Phe
20 25 30
Leu Pro Gly Phe Gln Gly Pro Leu Pro Phe Glu Leu Glu Thr Gly Tyr
35 40 45
Ile Gly Val Gly Glu Ala Glu Lys Asp Gln Met Phe Tyr Tyr Phe Ile
50 55 60
Lys Ser Glu Ser Asn Pro Glu Lys Asp Pro Leu Leu Leu Trp Leu Ser
65 70 75 80
Gly Gly Pro Phe Cys Ser Ser Phe Thr Ala Leu Ile Tyr Glu Asn Gly
85 90 95
Pro Ile Ala Phe Lys Ala Glu Glu Tyr Asn Gly Ser Ile Pro Ser Leu
100 105 110
Val Ser Thr Thr Tyr Ala Trp Thr Lys Val Ala Ser Ile Leu Tyr Leu
115 120 125
Asp Gln Pro Val Gly Thr Gly Phe Ser Tyr Ser Arg Asn Pro Leu Ala
130 135 140
Asp Ile Pro Ser Asp Thr Gly Val Ala Lys Pro Val Asn Glu Phe Leu
145 150 155 160
His Lys Trp Leu Asp Lys His Pro Glu Phe Leu Ser Asn Pro Leu Tyr
165 170 175
Val Ala Gly Asn Ser Tyr Ser Gly Ile Val Ile Pro Thr Ile Val Gln
180 185 190
Glu Ile Ser Asn Gly Asn His Leu Asp Ser Lys Pro Gln Ile Asn Leu
195 200 205
Gln Gly Phe Val Leu Gly Asn Pro Ala Thr Asp Thr Asp Ile Asp Leu
210 215 220
Asn Ser Arg Ile Pro Phe Ala His Gly Lys Ala Leu Ile Ser Asp Glu
225 230 235 240
His Tyr Glu Ser Leu Lys Arg Ser Cys Gln Gly Asn Tyr Ile Ser Val
245 250 255
Asn Pro Arg Asn Thr Lys Cys Leu Lys Leu Leu Glu Asp Phe Lys Lys
260 265 270
Cys Val Ser Gly Ile Ser Glu Glu Tyr Ile Leu Lys Pro Asp Cys Met
275 280 285
Trp Leu Tyr Ser Cys Met Ala Asn Leu His Ser Leu Ser Glu Tyr Trp
290 295 300
Ala Asn Glu Lys Ser Val Arg Lys Ala Leu Leu Val Asn Glu Gly Thr
305 310 315 320
Val Arg Lys Trp Ile Arg Cys Asn Thr Glu Ile Ala Tyr Asn Lys Asp
325 330 335
Ile Arg Ser Ser Val Pro Tyr His Lys Tyr Ile Ser Ile Glu Gly Tyr
340 345 350
Arg Ser Leu Val Phe Ser Gly Asp His Asp Met Leu Val Pro Phe Leu
355 360 365
Gly Thr Gln Ala Trp Ile Arg Ser Leu Asn Tyr Ser Ile Val Asp Asp
370 375 380
Trp Arg Pro Trp Met Val Gln Asn Gln Val Ala Gly Tyr Thr Arg Thr
385 390 395 400
Tyr Ala Asn Lys Met Thr Phe Ala Thr Val Lys Gly Gly Gly His Thr
405 410 415
Ser Glu Tyr Lys Pro Val Glu Thr Tyr Ile Met Ile Lys Arg Trp Leu
420 425 430
Ser Gly Gln Pro Leu
435
<210> 155
<211> 1410
<212> DNA
<213> Glycine max
<400> 155
atggaaaatt ttagttcaag ttacatttat tactgggttc tactaccctt ttttttgtta 60
tcacaattct cctttcaact tgcatggtgt ggctccatag taaagttcct tcctggattc 120
aagggacccc ttccttttgt acttgaaacc gggtatgtgg gagtgggtga atcagaggat 180
gtgcaggcat tctactactt cattgagtca gagaacaatc ccaagaaaga tcctctcatg 240
ctttggctca ctggtggccc tggttgctca gccttatctg gacttgtgtt tgaaatagga 300
ccacttacat tcaaatatga ggaatacaat gggagcctgc ccaatttggt cttgaggcca 360
cactcatgga caaaggttag tagcattata tttgtagact tgcctgtttc cacgggcttc 420
acttatgcca caacagagtt tgctgctcaa cgaagcgact ggattctagt tcaccaagtc 480
catcagtttc ttaggaagtg gttgattgat catccaaatt tttcgtcaaa tgaagtttac 540
attggtggcg attcatactc tggcattcct attccagtga ttgttcaaga aatttcacga 600
ggaaatgaaa aagggctcca accatggata aatctccagg gatacctgct gggaaatgca 660
gcaacaactc gaagggaaaa aaactatcaa attcccttcg ctcatggaat gggacttatt 720
tctgatgaac tatatgggtc actgcaaaaa aattgtaaag aagagtacat aaatgtagac 780
accagaaatg tattatgttc tagagatatc gagtcattca atgaggttac atcaggactt 840
aattcagccc atattttgga cccgtcatgt gagtggcttg atactgaaac atcttggagg 900
agatctctac ttaagaaata tcccagaaag aatttcctta atactcacct caaattggca 960
cccttaaact gtcggagtta tgtatacttc ctctgcggtt attgggccaa tgatgataat 1020
gttcgcactg cactgcacat ccgtaaggga agtataggaa aatggcatcg ttgtaccttc 1080
gatataccta acaagaagga tatctcaagc agctatgagt atcatgtaaa tctcagtaga 1140
aaaggctacc gttcgctgat atacagtggc gatcatgaca tgacaattcc tttcttggca 1200
actcaagcat ggataagatc tttaaactac tccattgtgg atgagtggag gcaatggcat 1260
acaaatggtc aagttgcagg atacacaagg acttactcca atcggatgac atttgcaact 1320
gtgaagggag gaggccacac agctccggag tacaagcctg atgaatgctt tgccatgttc 1380
agtaggtgga tatctaacag tgctttgtag 1410
<210> 156
<211> 469
<212> PRT
<213> Glycine max
<400> 156
Met Glu Asn Phe Ser Ser Ser Tyr Ile Tyr Tyr Trp Val Leu Leu Pro
1 5 10 15
Phe Phe Leu Leu Ser Gln Phe Ser Phe Gln Leu Ala Trp Cys Gly Ser
20 25 30
Ile Val Lys Phe Leu Pro Gly Phe Lys Gly Pro Leu Pro Phe Val Leu
35 40 45
Glu Thr Gly Tyr Val Gly Val Gly Glu Ser Glu Asp Val Gln Ala Phe
50 55 60
Tyr Tyr Phe Ile Glu Ser Glu Asn Asn Pro Lys Lys Asp Pro Leu Met
65 70 75 80
Leu Trp Leu Thr Gly Gly Pro Gly Cys Ser Ala Leu Ser Gly Leu Val
85 90 95
Phe Glu Ile Gly Pro Leu Thr Phe Lys Tyr Glu Glu Tyr Asn Gly Ser
100 105 110
Leu Pro Asn Leu Val Leu Arg Pro His Ser Trp Thr Lys Val Ser Ser
115 120 125
Ile Ile Phe Val Asp Leu Pro Val Ser Thr Gly Phe Thr Tyr Ala Thr
130 135 140
Thr Glu Phe Ala Ala Gln Arg Ser Asp Trp Ile Leu Val His Gln Val
145 150 155 160
His Gln Phe Leu Arg Lys Trp Leu Ile Asp His Pro Asn Phe Ser Ser
165 170 175
Asn Glu Val Tyr Ile Gly Gly Asp Ser Tyr Ser Gly Ile Pro Ile Pro
180 185 190
Val Ile Val Gln Glu Ile Ser Arg Gly Asn Glu Lys Gly Leu Gln Pro
195 200 205
Trp Ile Asn Leu Gln Gly Tyr Leu Leu Gly Asn Ala Ala Thr Thr Arg
210 215 220
Arg Glu Lys Asn Tyr Gln Ile Pro Phe Ala His Gly Met Gly Leu Ile
225 230 235 240
Ser Asp Glu Leu Tyr Gly Ser Leu Gln Lys Asn Cys Lys Glu Glu Tyr
245 250 255
Ile Asn Val Asp Thr Arg Asn Val Leu Cys Ser Arg Asp Ile Glu Ser
260 265 270
Phe Asn Glu Val Thr Ser Gly Leu Asn Ser Ala His Ile Leu Asp Pro
275 280 285
Ser Cys Glu Trp Leu Asp Thr Glu Thr Ser Trp Arg Arg Ser Leu Leu
290 295 300
Lys Lys Tyr Pro Arg Lys Asn Phe Leu Asn Thr His Leu Lys Leu Ala
305 310 315 320
Pro Leu Asn Cys Arg Ser Tyr Val Tyr Phe Leu Cys Gly Tyr Trp Ala
325 330 335
Asn Asp Asp Asn Val Arg Thr Ala Leu His Ile Arg Lys Gly Ser Ile
340 345 350
Gly Lys Trp His Arg Cys Thr Phe Asp Ile Pro Asn Lys Lys Asp Ile
355 360 365
Ser Ser Ser Tyr Glu Tyr His Val Asn Leu Ser Arg Lys Gly Tyr Arg
370 375 380
Ser Leu Ile Tyr Ser Gly Asp His Asp Met Thr Ile Pro Phe Leu Ala
385 390 395 400
Thr Gln Ala Trp Ile Arg Ser Leu Asn Tyr Ser Ile Val Asp Glu Trp
405 410 415
Arg Gln Trp His Thr Asn Gly Gln Val Ala Gly Tyr Thr Arg Thr Tyr
420 425 430
Ser Asn Arg Met Thr Phe Ala Thr Val Lys Gly Gly Gly His Thr Ala
435 440 445
Pro Glu Tyr Lys Pro Asp Glu Cys Phe Ala Met Phe Ser Arg Trp Ile
450 455 460
Ser Asn Ser Ala Leu
465
<210> 157
<211> 1038
<212> DNA
<213> Oryza sativa
<400> 157
atgaagaatc gtattctgga gggagctctt atgtgccaga tttttcaggt tagttataag 60
ctgagagaag gaaatagagt agctcataga cttgctgatt tggggtcctt tgtgcctgtt 120
atagtgaagt ttactaggct caaaatcttt ctggttccgc tactgatcag acttgatgag 180
gatggctctg aattaagtga tcggaatggc aagaagatga atgagccgag acgatgccgc 240
cacgcaaatg acgttgcgtg cgtgcgtgac gccgccgccg aaacccacgc tggaattttt 300
actaccgcca gaactcctgc tcaagatcgt cgctcgctcg gacgctgcca cactcgtccg 360
ctcccagagg acgatgcgac tgaggaggcg gacaaggcgg cgacggcggg ggatacggac 420
ggtgacggag ggggcggaca aggcggtgac ggcggggata cggacgatga cggaggggac 480
ggacaaggcg gcgaggccgg gagaggaata gcgaggaggc atcaggaatt gtcatttcgc 540
cgagatggcg cacgcaacac tgcccagaga tttcgaaacc aaagcacact agatctggcc 600
ctgccgccgc cagagctgac cggcgtctac gcggcggcgg ggaggtggga ggcggaggtg 660
cggaggtggc ggccagaggc agccgggcct gcgcctggcc gtgtggattg gggatggctg 720
gaggtggtcg ggagggcggt ggagagtggc aagacgttag tgatggagtc cctctggatg 780
acggtagggg cggcgaagca cggctgtggc ctagtggcag cggctcccgt cggcgccaat 840
ggccctaggg gctgcggcgg cccaggaggg cggcgtctcc cgtcgccgcc ggcagcccta 900
ggggctgctg cggaagacgg cgttggtgcc ggtggcccag ggcagcggag cactgtgaat 960
ccgatgaagt ggaagccgat cccgtcggtt cgtgtatggt tgactagttc cagccaaacg 1020
acgaatgacg acggttga 1038
<210> 158
<211> 345
<212> PRT
<213> Oryza sativa
<400> 158
Met Lys Asn Arg Ile Leu Glu Gly Ala Leu Met Cys Gln Ile Phe Gln
1 5 10 15
Val Ser Tyr Lys Leu Arg Glu Gly Asn Arg Val Ala His Arg Leu Ala
20 25 30
Asp Leu Gly Ser Phe Val Pro Val Ile Val Lys Phe Thr Arg Leu Lys
35 40 45
Ile Phe Leu Val Pro Leu Leu Ile Arg Leu Asp Glu Asp Gly Ser Glu
50 55 60
Leu Ser Asp Arg Asn Gly Lys Lys Met Asn Glu Pro Arg Arg Cys Arg
65 70 75 80
His Ala Asn Asp Val Ala Cys Val Arg Asp Ala Ala Ala Glu Thr His
85 90 95
Ala Gly Ile Phe Thr Thr Ala Arg Thr Pro Ala Gln Asp Arg Arg Ser
100 105 110
Leu Gly Arg Cys His Thr Arg Pro Leu Pro Glu Asp Asp Ala Thr Glu
115 120 125
Glu Ala Asp Lys Ala Ala Thr Ala Gly Asp Thr Asp Gly Asp Gly Gly
130 135 140
Gly Gly Gln Gly Gly Asp Gly Gly Asp Thr Asp Asp Asp Gly Gly Asp
145 150 155 160
Gly Gln Gly Gly Glu Ala Gly Arg Gly Ile Ala Arg Arg His Gln Glu
165 170 175
Leu Ser Phe Arg Arg Asp Gly Ala Arg Asn Thr Ala Gln Arg Phe Arg
180 185 190
Asn Gln Ser Thr Leu Asp Leu Ala Leu Pro Pro Pro Glu Leu Thr Gly
195 200 205
Val Tyr Ala Ala Ala Gly Arg Trp Glu Ala Glu Val Arg Arg Trp Arg
210 215 220
Pro Glu Ala Ala Gly Pro Ala Pro Gly Arg Val Asp Trp Gly Trp Leu
225 230 235 240
Glu Val Val Gly Arg Ala Val Glu Ser Gly Lys Thr Leu Val Met Glu
245 250 255
Ser Leu Trp Met Thr Val Gly Ala Ala Lys His Gly Cys Gly Leu Val
260 265 270
Ala Ala Ala Pro Val Gly Ala Asn Gly Pro Arg Gly Cys Gly Gly Pro
275 280 285
Gly Gly Arg Arg Leu Pro Ser Pro Pro Ala Ala Leu Gly Ala Ala Ala
290 295 300
Glu Asp Gly Val Gly Ala Gly Gly Pro Gly Gln Arg Ser Thr Val Asn
305 310 315 320
Pro Met Lys Trp Lys Pro Ile Pro Ser Val Arg Val Trp Leu Thr Ser
325 330 335
Ser Ser Gln Thr Thr Asn Asp Asp Gly
340 345

Claims (19)

1.一种分离的多核苷酸，其包含的多核苷酸编码的多肽的氨基酸序列与SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158有至少90％的序列一致性，其中植物中所述多核苷酸表达的增加提高了耐旱性和产量。
2.权利要求1所述的分离的多核苷酸，其中所述多核苷酸含有的核苷酸序列为SEQ IDNO:1、SEQ ID NO:2、SEQ ID NO:4、SEQ ID NO:6、SEQ ID NO:7、SEQ ID NO:9、SEQ ID NO:10、SEQ ID NO:12、SEQ ID NO:13、SEQ ID NO:15、SEQ ID NO:16、SEQ ID NO:18、SEQ IDNO:19、SEQ ID NO:21、SEQ ID NO:22、SEQ ID NO:24.SEQ ID NO:25、SEQ ID NO:27、SEQ IDNO:28、SEQ ID NO:30、SEQ ID NO:31、SEQ ID NO:33、SEQ ID NO:34、SEQ ID NO:36、SEQ IDNO:37、SEQ ID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ IDNO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:89、SEQ IDNO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ ID NO:101、SEQID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117、SEQ ID NO:119、SEQ ID NO:121、SEQ ID NO:123、SEQID NO:125、SEQ ID NO:127、SEQ ID NO:129、SEQ ID NO:131、SEQ ID NO:133、SEQ ID NO:135、SEQ ID NO:137、SEQ ID NO:139、SEQ ID NO:141、SEQ ID NO:143、SEQ ID NO:145、SEQID NO:147、SEQ ID NO:149、SEQ ID NO:151、SEQ ID NO:153、SEQ ID NO:155、SEQ ID NO:157。
3.权利要求1所述的多核苷酸，其中所述编码的多肽含有的氨基酸序列为SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158。
4.权利要求1-3所述的任何分离的多核苷酸，植物中所述多核苷酸表达的增加提高了干旱条件下的耐旱性和/或产量。
5.一种重组DNA构建体，其包含有权利要求1到3所述的任何分离的多核苷酸，可操作地连接至少一个异源调控元件。
6.权利要求5所述的重组DNA构建体，其中所述的调控元件是一个异源启动子。
7.一种改良的植物或种子，其包含的至少一种编码多肽的多核苷酸表达的增加，所述多肽包含的氨基酸序列与SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158有至少90％的序列一致性。
8.权利要求7所述的植物，该植物在其基因组中含有一个重组DNA构建体，所述重组DNA构建体含有权利要求1到3任意的多核苷酸可操作地连接至少一个调控元件，其中所述植物与对照植物相比较时表现出提高的耐旱性。
9.权利要求7所述的植物，该植物在其基因组位点上包含有一个靶向基因修饰，所述靶向基因修饰的基因组位点包含有编码多肽的多核苷酸，该多肽的氨基酸序列与SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158有至少90％的序列一致性。因而增加多肽的表达，所述植物在与对照植物相比较时表现出提高的耐旱性。
10.权利要求7-9所述的任何植物，其中所述植物选自水稻、玉米、大豆、向日葵、高粱、油菜、小麦、苜蓿、棉花、大麦、小米、甘蔗和柳枝稷。
11.一种增加植物耐旱性的方法，包括提高至少一个编码多肽的多核苷酸的表达，所述多肽含有的氨基酸序列与SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158有至少90％的序列一致性。
12.权利要求11所述的方法，该方法包括：
(a)在可再生植物细胞中表达含有可操作地连接多核苷酸序列的调控元件的重组DNA构建体；和
(b)再生所述植物，该植物在其基因组中含有重组DNA构建体。
13.权利要求11所述的方法，该方法包括：
(a)向可再生植物细胞的基因组位点上引入一个靶向基因修饰，该基因组位点编码含有氨基酸序列的多肽，所述氨基酸序列与SEQ ID NOs:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158相比有至少90％的序列一致性；和
(b)再生所述植物，植物中所述多肽的表达水平和/或活性是增加的。
14.权利要求13所述的方法，其中靶向基因修饰的引用使用了以下基因修饰技术：多核苷酸引导的核酸内切酶、CRISPR-Cas核酸内切酶、碱基编辑脱氨酶、锌指核酸酶、转录激活物样效应器核酸酶(TALEN)、工程化位点特异性巨核酸酶或Argonaute。
15.权利要求13所述的方法，其中所述靶向基因修饰存在于基因组位点的(a)编码区；(b)非编码区；(c)调控序列；(d)非翻译区；(e)任何(a)-(d)的组合，以编码含有氨基酸序列的多肽，所述氨基酸序列与选自SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158的这些氨基酸序列相比较有至少90％的一致性。
16.权利要求12所述的方法，其中所述调控元件是一种异源启动子。
17.增加植物耐旱性的方法，所述方法包括：
(a)在一个可再生植物细胞中表达一种可操作地连接有至少一个异源调控元件的多核苷酸，其中所述多核苷酸编码的多肽的氨基酸序列与SEQ ID NO:3、5、8、11、14、17、20、23、26、29、32、35、38、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156或158有至少90％的序列一致性，且与对照植物相比，该多核苷酸的表达水平是增加的；和
(b)与不包含有可操作地连接到异源调节元件的多核苷酸的对照植物相比，选择包含可操作地连接到异源调节元件的多核苷酸的植物以提高耐旱性。
18.权利要求17所述的方法，其中所述异源调控元件是一种启动子。
19.权利要求17或18所述的方法，其中所述植物选自水稻、玉米、大豆、向日葵、油菜、小麦、苜蓿、棉花、大麦、小米、甘蔗和柳枝稷。
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