CN114245824A

CN114245824A - 非生物胁迫耐受性提高的植物和方法

Info

Publication number: CN114245824A
Application number: CN201980096678.5A
Authority: CN
Inventors: 吕贵华; 王国奎; 毛冠凡; 张玉; 王昌贵; 陈光武; 高阳
Original assignee: Sinobioway Bio Agriculture Group Co Ltd; Pioneer Overseas Corp
Current assignee: Sinobioway Bio Agriculture Group Co Ltd; Pioneer Overseas Corp
Priority date: 2019-05-22
Filing date: 2019-05-22
Publication date: 2022-03-25
Also published as: US20220186245A1; WO2020232661A1; US11976289B2

Abstract

分离的多核苷酸和多肽以及重组DNA构建体能赋予增加的耐旱性和产量。本发明提供了包含有这些重组DNA构建体的组合物如植株或种子，以及利用这些重组DNA构建体的方法。所述重组DNA构建体包含有一个多核苷酸可操作地连接一个植物中功能性的启动子，其中所述多核苷酸编码耐旱多肽。

Description

非生物胁迫耐受性提高的植物和方法

技术领域

本发明涉及植物育种和遗传学等领域，尤其是，涉及到提高植物对非生物胁迫的耐受性。

发明背景

造成植物胁迫的原因可能同时有生物因素和非生物因素。生物因素的胁迫包括病毒感染、昆虫取食和植物寄生如榭寄生等；非生物因素胁迫包括水分的过量或不足、极端气候、除草剂等化学合成物。

非生物胁迫是引发全球作物减产的主要原因，导致主要农作物的平均产量减少50％以上(Boyer,J.S.(1982)Science 218:443-448；Bray,E.A.等(2000)In Biochemistryand Molecular Biology of Plants,Buchannan,B.B.等编著,Amer.Soc.Plant Biol.,pp.1158-1249)。因此，就需要开发能够提高植物非生物胁迫耐受性的组合物和方法，本发明提供了这种组合物和方法。

发明概述

以下实施例包括在本发明中涵盖的所有实施例：

在一个实施例中，本发明包括一种分离的多核苷酸，所述多核苷酸包含有一个多肽，其氨基酸序列与SEQ ID NO：3、6、9、12、15、18、21或24有至少90％的一致性。并且提高植物中所述多核苷酸的表达能提高植物的耐旱性。在某些实施例中，所述分离的多核苷酸编码SEQ ID NO：3、6、9、12、15、18、21或24氨基酸序列。在某些实施例中，所述分离的多核苷酸包括SEQ ID NO：1、2、4、5、7、8、10、11、13、14、16、17、19、20、22或23核苷酸序列。在某些实施例中，干旱条件下提高植物中所述多核苷酸的表达能提高籽粒的产量。

本发明还提供了一种重组DNA构建体，所述重组DNA构建体包括一个分离的多核苷酸可操作地连接至少一个异源调控元件。其中，所述多核苷酸包含有一个多肽，所述多肽的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21或24有至少90％的一致性。

本发明进一步提供了一种改良的植物或种子，所述植物或种子能够提高至少一种多核苷酸的表达量或活性。所述多核苷酸包含有一种多肽，所述多肽的氨基酸序列与SEQID NO:3、6、9、12、15、18、21或24有至少90％一致性。在某些实施例中，所述改良的植物或种子在其基因组中包含有一种重组DNA构建体，所述重组DNA构建体包含有一个多核苷酸操作地连接至少一个异源调控元件。其中所述多核苷酸编码一种多肽，这种多肽的氨基酸序列与SEQ ID NO：3、6、9、12、15、18、21或24有至少90％的序列一致性。在某些实施例中，所述改良的植物生长在干旱条件下时，与对照植物相比，明显有更高的干旱耐受性和更多的籽粒产量。

在某些实施例中，所述改良的植物或种子在其基因组位点上包含有一种靶向遗传修饰。所述的基因组位点包含有一种多核苷酸，所述多核苷酸编码的多肽的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21或24有至少90％的序列一致性。所述的靶向遗传修饰能增加所述多肽的表达和/或活性。在某些实施例中，所述改良的植物生长在干旱条件下时，与对照植物相比，表现出提高的干旱耐受性和增加的籽粒产量。

在某些实施例中，所述植物包括水稻、玉米、大豆、向日葵、高粱、油菜、小麦、苜蓿、棉花、大麦、粟、甘蔗和柳枝稷。

还提供了增加植物耐旱性的方法，所述方法包括提高至少一个多核苷酸的表达。所述多核苷酸编码的多肽的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21或24有至少90％的序列一致性。其中，与对照植物相比，所述获得的植物表现出增加的耐旱性。

在某些实施例中，所述能够提高耐旱性的方法包括：(a)向可再生植物细胞引入一个重组DNA构建体。所述重组DNA构建体包含有一个多核苷酸可操作地连接至少一个异源调控元件，其中所述多核苷酸编码的一个多肽的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21或24相比至少有80％的序列一致性；和(b)获得所述植物的子代植物，其中所述植物基因组中含有所述重组DNA构建体。

在某些实施例中，所述能够提高耐旱性的方法包括：(a)向可再生植物细胞的基因组位点引入一个靶向遗传修饰。所述靶向遗传修饰包含一个多核苷酸，所述多核苷酸编码的一个多肽的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21或24相比至少有80％的序列一致性；和(b)获得所述植物的子代植物。其中，所述植物在其基因组中包含有所述引入的遗传修饰，并且能够提高所述多肽的表达量和/或活性。在某些实施例中，所述靶向遗传修饰的引入是通过多核苷酸引导的核酸内切酶、CRISPR-Cas核酸内切酶、碱基编辑脱氨酶、锌指核酸酶、转录激活因子样效应核酸酶(TALEN)、工程位点特异性大范围核酸酶或Argonaute等基因组修饰技术。在某些实施例中，所述靶向遗传修饰存在于(a)所述编码区；(b)非编码区；(c)调控序列；(d)非翻译区；或(e)基因组位点上(a)-(d)的任意组合，所述基因组位点编码的多肽包含的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21或24相比有至少80％的序列一致性。

附图说明及序列表

根据以下发明详述和附图及序列表，可以更全面的理解本发明，以下发明详述和附图及序列表形成本发明的一部分。

表1序列表中核苷酸和氨基酸序列的编号

序列描述以及关联的序列表遵循如37C.F.R.§1.821-1.825中所列出的管理专利申请中的核苷酸和/或氨基酸序列公开的规则。序列表包含核苷酸序列字符的单字母码以及氨基酸的三字母码，用于核苷酸和氨基酸序列数据的符号和格式遵循37C.F.R.§1.822中列出的规则。

详细描述

本发明中所列出的每篇参考文献的公开内容的全文均以引用的方式并入本发明。

如本发明所用的并在所附权利要求书中的单数形式“一个”和“所述”包括复数涵义，除非上下文中清楚地另有指明。因此，例如，“一株植物”的涵义包括多株该类植物。“一个细胞”的涵义包括一个或多个细胞及其本领域的技术人员已知的等同物，等等。

定义：

如本文所述，植物“提高的耐旱性”是指任何在生理或物理表型上可测量的提高。比如在干旱条件下，相对于参照植物或对照植物进行的产量测定。通常，一种基因组中包含有一个重组DNA构建体或DNA修饰的植物与基因组中不含有重组DNA构建体或DNA修饰的参照植物或对照植物相比，表现出增加的耐旱性。

植物“增加的百草枯耐受性”反映了所述植物在经过百草枯溶液处理后，与参照植物或对照植物相比，发生更少的生理或物理伤害并存活下来的能力。一般来说，对于相对低水平的百草枯的耐受性可以作为对非生物胁迫耐受性的标志，比如干旱胁迫。

“农艺性状”作为可测量的参数包括但不限于：绿量、籽粒产量、生长率、总生物量或累积速率、成熟期鲜重、成熟期干重、果实产量、种子产量、植株总氮素含量、果实氮素含量、种子氮素含量、叶片组织氮素含量、植物总游离氨基酸含量、果实游离氨基酸含量、种子游离氨基酸含量、叶片组织游离氨基酸含量、植物总蛋白含量、耐旱性、营养吸收速率、根倒伏、收获指数、茎倒伏、株高、穗位高、穗长、耐盐碱、分蘖数、圆锥花序大小、幼苗活力和低温出苗率。

“转基因的”指其基因组因异源核酸(如重组DNA构建体)的存在而发生改变的任何细胞、细胞系、愈伤组织、组织、植物部分或植物，例如重组DNA构建体，包括那些最初的转基因事件以及从最初的转基因事件通过有性杂交或无性生殖而产生的那些。如本发明所用的术语“转基因”不涵盖通过常规植物育种方法或通过诸如随机异花受精、非重组病毒感染、非重组细菌转化、非重组转座或自发突变之类的自然发生事件导致的基因组(染色体基因组或染色体外基因组)改变。

“对照”、“对照植物”或“对照植物细胞”为测定受测试植物或植物细胞的表型变化提供参考，由于转化，受测植物或植物细胞的基因组改变影响到目的基因，测试的植物或植物细胞可以是转基因植物或转基因植物细胞的子代。例如，对照植物可能和受测试植物有着相同的基因背景，但是没有导致受测试植物或细胞发生所述的遗传变异。

“植物”包括植物全部植株、植物器官、植物组织、种子和植物细胞以及相同的子代植物。植物细胞包括但不限于来自于以下物质的细胞：种子、悬浮培养物、胚、分生区域、愈伤组织、叶片、根系、芽、配子体、孢子体、花粉和小孢子。

“子代”包括植物的任何后续世代。

“修饰的植物”包括在其基因组内有异源多核苷酸或修饰的基因或启动子的植物。例如，所述异源多核苷酸能够稳定整合进基因组内，并遗传连续的世代。所述异源多核苷酸能单独地或作为重组DNA构建体的部分被整合进基因组内。

对于序列而言的“异源”是指一个起源于外来物种的序列，是通过蓄意的人为干预而从其天然形式发生了组成和/或基因座的显著改变的序列。

“多核苷酸”、“核酸序列”、“核苷酸序列”和“核酸片段”都可以互换并且是任选含有合成的、非天然的或改变的核苷酸碱基的单链或双链RNA或DNA聚合物。核苷酸(通常以它们的5′-单磷酸形式存在)通过如下它们的单个字母名称来指代：“A”为腺苷酸或脱氧腺苷酸(分别对应RNA或DNA)，“C”表示胞苷酸或脱氧胞苷酸，“G”表示鸟苷酸或脱氧鸟苷酸，“U”表示尿苷酸，“T”表示脱氧胸苷酸，“R”表示嘌呤(A或G)，“Y”表示嘧啶(C或T)，“K”表示G或T，“H”表示A或C或T，“I”表示肌苷，并且“N”表示任何核苷酸。

“多肽”、“肽”、“氨基酸序列”和“蛋白质”在本发明中可互换使用，指氨基酸残基的聚合物。该术语适用于其中一个或多个氨基酸残基是相应的天然存在的氨基酸的人工化学类似物的氨基酸聚合物，以及适用于天然存在的氨基酸聚合物。术语“多肽”、“肽”、“氨基酸序列”和“蛋白质”还可包括修饰形式，包括但不限于糖基化、脂质连接、硫酸盐化、谷氨酸残基的γ羧化、羟化和ADP-核糖基化。

“重组DNA构建体”是指一个在自然界中通常不会一起存在的核酸片段的组合。因此，一个重组DNA构建体可能包含来源于不同物种的调控元件和编码序列；或者是来源于同一物种以一种不同于通常在自然界中发现的方式排列的调控元件和编码序列。

“调控元件”是指位于编码序列的上游(5'-非编码序列)、中间或下游(3'-非编码序列)，并且影响相关编码序列的转录、RNA加工或稳定性或者翻译的核苷酸序列。调控元件包括但不限于：启动子、翻译前导序列、内含子、多腺苷酸化识别序列。术语“调控元件”和“调控序列”和“调控区”在本文中可以互换使用。

“启动子”是指能控制另一个核酸片段转录的核酸片段。“植物中有功能的启动子”是能够控制植物细胞中基因转录的启动子，无论是否来源于植物细胞。“组织特异性启动子”和“组织优选启动子”是指占主导但不是必要的唯一的在一种组织或器官中表达，而且也可以在一种特定的细胞或细胞型中表达的启动子。“发育调控启动子”是一种其活性由生长期来决定的启动子。

“可操作地连接”是指核酸片段连接成单一片段，使得其中一个核酸片段的功能受到另一个核酸片段的调控。例如，在启动子能够调控一个核酸片段的转录时，该启动子与该核酸片段进行了可操作地连接。

“表达”是指功能产物的产生。例如，核酸片段的表达可指核酸片段的转录(如转录生成功能RNA或mRNA)，和/或mRNA翻译成前体或成熟蛋白质。

本文中“增加”、“提高”或类似词汇是指实验组(如本文中描述的有DNA修饰的植物)中与对照组(如不含有DNA修饰的野生型植物)进行比较时，任何可检测到的增加。因此，蛋白质表达的增加包括样本中蛋白质总水平的任何可检测的增加，可以使用本领域中的常规方法如蛋白质印迹法和ELISA来确定。

本文中使用的“产量”是指单位面积收获的农产品的总量，并且可根据谷物水分(如玉米通常为15％，水稻为13.5％)进行调整收获时每英亩作物的立方数或每亩作物的千克数。谷物水分在收获时测定。调整后的谷物重量的测试是确定为每株的磅或克的重量，由收获时的谷物水分来进行调整。

本发明中，当在特定比对窗口中最大一致性比对时，两个多聚核苷酸或多肽序列的“序列一致性”或“一致性”指两序列中残基相同。当序列一致性百分数用于提及的蛋白质，被认为不同残基位置与保守氨基酸替代不同，氨基酸残基被具有相似化学性质的其它氨基酸残基(如电荷或疏水性)替代，而不改变分子的功能特性。当序列在保守区替代不同，序列一致性百分数可以上调以更正替代的保守性。保守替代不同的序列被认为具有“序列相似性”或“相似性”。本领域的技术人员熟悉进行调整的方法，包括计算作为部分的而不是全长错配的保守替代，因而增加序列一致性百分数，因此，相同氨基酸给与1分，不保守的替代给与0分，保守替代给与0-1分，计算保守替代的得分，如在PC/GENE执行(Intelligenetics，Mountain View，加州)。

本发明中，“序列一致性百分数”是通过确定两个序列中一致的核酸碱基或氨基酸残基位置的数量计算出匹配位置的数量，匹配位置的数量除以对比窗口中位置的总量，并用结果乘以100。

除非特别说明，本发明中的多序列比对采用Clustal V比对方法(Higgins和Sharp.(1989)CABIOS.5：151-153)和缺省参数(间隙罚分＝10，间隙长度罚分＝10)，两个比对的缺省参数和计算氨基酸序列一致性百分数使用Clustal V方法：KTUPLE＝1，间隙罚分＝3，窗口＝5和对角线拯救＝5；用于核酸序列的参数KTUPLE＝2，间隙罚分＝5，窗口＝4和对角线拯救＝4。序列比对后应用Clustal V程序，通过观察“序列距离”表，可以得到“一致性百分比”和“散度”值，除非特别说明，一致性百分比和散度值以相同的方式获得。

组合物：

多核苷酸和多肽

本发明中提供的多核苷酸编码的多肽如下：

一方面，本发明提供了一个多核苷酸，所述多核苷酸编码的多肽包含有一个氨基酸序列，所述氨基酸序列与以下任一氨基酸序列有至少80％(例如，81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、或100％)的一致性：SEQ ID NO:3(OsDN-DTP8)、SEQ ID NO:6(OsKIK1)、SEQ ID NO:9(OsRWDD1)、SEQ ID NO:12(OsUFD1)、SEQ ID NO:15(OsPPR2)、SEQ ID NO:18(OsSAUR28-1)、SEQ ID NO:21(OsHYS1)和SEQ ID NO:24(OsFBID1)。

“OsDN-DTP8”是指一种在过度表达时能够赋予水稻耐旱性状的多肽。所述OsDN-DTP8多肽(SEQ ID NO:3)是由水稻基因位点LOC_Os09g04650.1上的编码序列(CDS)(SEQ IDNO:2)或核苷酸序列(SEQ ID NO:1)编码，其在TIGR中的注释为“表达蛋白”。“OsDN-DTP8多肽”在本文中是指OsDN-DTP8多肽及其旁系同源基因(如由SEQ ID NO:51编码的SEQ ID NO:52)或来自于其它生物的同源基因。

“OsKIK1”是指一种在过量表达时能赋予水稻耐旱性状的多肽。所述OsKIK1多肽(SEQ ID NO:6)是由水稻基因位点LOC_Os07g36570.1上的编码序列(CDS)(SEQ ID NO:5)或核苷酸序列(SEQ ID NO:4)编码，其在TIGR中注释为的“KI域相互作用激酶1，推定，表达”。“KI多肽”在本文中是指OsKIK1多肽及其旁系同源基因(如由SEQ ID NO:53编码的SEQ IDNO:54)，以及来自于其它生物的同源基因，例如玉米的同源基因(例如，由SEQ ID NO:55编码的SEQ ID NO:56)、高粱的同源基因(例如，由SEQ ID NO:57编码的SEQ ID NO:58)、拟南芥的同源基因(例如，由SEQ ID NO:59编码的SEQ ID NO:60)，或大豆的同源基因(例如，由SEQ ID NO:61编码的SEQ ID NO:62)。

“OsRWDD1”是指一种在过量表达时能赋予水稻耐旱性状的多肽。所述OsRWDD1多肽(SEQ ID NO:9)是由水稻基因位点LOC_Os02g46740.1上的编码序列(CDS)(SEQ ID NO:8)或核苷酸序列(SEQ ID NO:7)编码，其在TIGR中的注释为“含有RWD结构域的蛋白质，表达”。“RWDD1多肽”在本文中是指OsRWDD1多肽及其旁系同源基因(如由SEQ ID NO:63编码的SEQID NO:64)，以及来自于其它生物的同源基因，例如玉米的同源基因(例如，由SEQ ID NO:65编码的SEQ ID NO:66)、高粱的同源基因(例如，由SEQ ID NO:67编码的SEQ ID NO:68)、拟南芥的同源基因(例如，由SEQ ID NO:69编码的SEQ ID NO:70)，或大豆的同源基因(例如，由SEQ ID NO:71编码的SEQ ID NO:72)。

“OsUFD1”是指一种在过量表达时能赋予水稻耐旱性状的多肽。所述OsUFD1多肽(SEQ ID NO:12)是由水稻基因位点LOC_Os01g68940.1上的编码序列(CDS)(SEQ ID NO:11)或核苷酸序列(SEQ ID NO:10)编码，其在TIGR中的注释为“泛素家族结构域蛋白，表达”。“UFD1多肽”在本文中是指OsUFD1多肽及其旁系同源基因(如由SEQ ID NO:73编码的SEQ IDNO:74)，以及来自于其它生物的同源基因，例如玉米的同源基因(例如，由SEQ ID NO:75编码的SEQ ID NO:76)、高粱的同源基因(例如，由SEQ ID NO:77编码的SEQ ID NO:78)、拟南芥的同源基因(例如，由SEQ ID NO:79编码的SEQ ID NO:80)，或大豆的同源基因(例如，由SEQ ID NO:81编码的SEQ ID NO:82)。

“OsPPR2”是指一种在过量表达时能赋予水稻耐旱性状的多肽。所述OsPPR2多肽(SEQ ID NO:15)是由水稻基因位点LOC_Os03g06910.1上的编码序列(CDS)(SEQ ID NO:14)或核苷酸序列(SEQ ID NO:13)编码，其在TIGR中的注释为“含PPR重复序列的蛋白质”。“PPR2多肽”在本文中是指OsPPR2多肽及其旁系同源基因(如由SEQ ID NO:83编码的SEQ IDNO:84)，以及来自于其它生物的同源基因，例如玉米的同源基因(例如，由SEQ ID NO:85编码的SEQ ID NO:86)、高粱的同源基因(例如，由SEQ ID NO:87编码的SEQ ID NO:88)、拟南芥的同源基因(例如，由SEQ ID NO:89编码的SEQ ID NO:90)，或大豆的同源基因(例如，由SEQ ID NO:91编码的SEQ ID NO:92)。

“OsSAUR28-1”是指一种在过量表达时能赋予水稻耐旱性状的多肽。所述OsSAUR28-1多肽(SEQ ID NO:18)是由水稻基因位点LOC_Os06g48860.1上的编码序列(CDS)(SEQ ID NO:17)或核苷酸序列(SEQ ID NO:16)编码，其在TIGR中的注释为“OsSAUR28生长素反应性SAUR基因家族成员，表达”。“SAUR28-1多肽”在本文中是指OsSAUR28-1多肽及其旁系同源基因，以及来自于其它生物的同源基因，例如玉米的同源基因(例如，由SEQ ID NO:93编码的SEQ ID NO:94)、高粱的同源基因(例如，由SEQ ID NO:95编码的SEQ ID NO:96)、拟南芥的同源基因(例如，由SEQ ID NO:97编码的SEQ ID NO:98)，或大豆的同源基因(例如，由SEQ ID NO:99编码的SEQ ID NO:100)。

“OsHYS1”是指一种在过量表达时能赋予水稻耐旱性状的多肽。所述OsHYS1多肽(SEQ ID NO:21)是由水稻基因位点LOC_Os01g68970.1上的编码序列(CDS)(SEQ ID NO:20)或核苷酸序列(SEQ ID NO:19)编码，其在TIGR中的注释为“HYS1,推定，表达”。“HYS1多肽”在本文中是指OsHYS1多肽及其旁系同源基因(如由SEQ ID NO:101编码的SEQ ID NO:102)，以及来自于其它生物的同源基因，例如玉米的同源基因(例如，如由SEQ ID NO:103编码的SEQ ID NO:104)、高粱的同源基因(例如，由SEQ ID NO:105编码的SEQ ID NO:106)、拟南芥的同源基因(例如，由SEQ ID NO:107编码的SEQ ID NO:108)，或大豆的同源基因(例如，由SEQ ID NO:109编码的SEQ ID NO:110)。

“OsFBID1”是指一种在过量表达时能赋予水稻耐旱性状的多肽。所述OsFBID1多肽(SEQ ID NO:24)是由水稻基因位点LOC_Os04g31570.1上的编码序列(CDS)(SEQ ID NO:23)或核苷酸序列(SEQ ID NO:22)编码，其在TIGR中的注释为“F-box蛋白交换域蛋白，表达”。“FBID1多肽”在本文中是指FBID1多肽及其旁系同源基因(如由SEQ ID NO:111编码的SEQID NO:112)，以及来自于其它生物的同源基因。

正如本领域内的技术人员所意识到的，本发明中包含的不仅仅是特定的典型性序列。核酸片段的改变导致在给定位点产生化学等效氨基酸，但不影响编码多肽的功能性质，这在本领域是众所周知的。例如，氨基酸丙氨酸(疏水性氨基酸)的密码子可由编码另一疏水性较小的残基(例如甘氨酸)或疏水性较大的残基(例如缬氨酸、亮氨酸或异亮氨酸)的密码子取代。类似地，导致一个带负电的残基替换另一个残基的变化，如用天冬氨酸替换谷氨酸，或一个带正电的残基替换另一个残基，如用赖氨酸替换精氨酸，也可预期产生功能等效的产物。导致多肽分子N端和C端部分改变的核苷酸变化也不会改变多肽的活性。所提出的每一个改良都在本领域的常规技术范围内，确定编码产品的生物活性的保留也是如此。

重组DNA构建体

还提供了包含有本文中所描述的任何多核苷酸的重组DNA构建体。在某些实例中，所述重组DNA构建体进一步包含有至少一个调控元件。在某些实例中所述的至少一个调控元件是一种异源调控元件。在某些实例中，重组DNA构建体中的所述至少一个调控田间包含有一个启动子。在某些势力中，所述启动子是一种异源启动子。

许多启动子能被用于本文中的重组DNA构建体。所述启动子能依据所期望的结果进行选择，并且可以包括用于在宿主生物体中表达的组成型、组织特异性、诱导型或其他启动子。

“组成型”启动子是一种在大多数环境条件下活跃的启动子。组成型启动子包括，例如，WO 99/43838和美国专利号6072050中公开的Rsyn7启动子和其他组成型启动子的核心启动子；核心CaMV 35S启动子(Odell等(1985)Nature 313:810-812)；水稻肌动蛋白(McElroy等(1990)Plant Cell 2:163-171)；泛素(Christensen等(1989)PlantMol.Biol.12:619-632和Christensen等(1992)Plant Mol.Biol.18:675-689)；pEMU(Last等(1991)Theor.Appl.Genet.81:581-588)；MAS(Velten等(1984)EMBO J.3:2723-2730)；ALS启动子(美国专利号5659026)等。其他组成型启动子包括例如美国专利号5608149；5608144；5604121；5569597；5466785；5399680；5268463；5608142；和6177611。

组织特异性或生育调控启动子是一种DNA序列，可以选择性调控植物细胞或植物组织(例如在这些细胞或组织的穗发育、结实或两者都至关重要的关键时期)中DNA序列的表达，并且通常这种DNA序列的表达仅限于在植物的重要发育期，如穗的发育或种子的成熟时期。任何可识别的能够引起期望的时间和空间表达的启动子都将用于本文的方法中。

许多叶片优选启动子是本领域中已知的(Yamamoto等(1997)Plant J.12(2)：255-265；Kwon等(1994)Plant Physiol，105:357-367；Yamamoto等(1994)Plant Cell Physiol，35(5):773-778；Gotor等(1993)Plant J.3:509-518；Orozco等(1993)Plant Mol.Biol，23(6):1129-1138；和Matsuoka等(1993)Proc.Natl.Acad.Sci.USA，90(20)：9586-9590)。

种子或胚胎特异性且可用于本公开的启动子，包括大豆Kunitz胰蛋白酶抑制剂(Kti3、Jofuku和Goldberg，(1989)Plant Cell 1:1079-1093)，豌豆球蛋白和豆科植物(豌豆子叶)(Rerie、W.G等人(1991)Mol.Gen.Genet.259:149-157；Newbigin、E.J等人(1990)Planta 180:461-470；Higgins、T.J.V等人(1988)Plant.Mol.Biol.11:683-695)，玉米醇溶蛋白(玉米胚乳)(Schemthaner、J.P等人(1988)EMBO J.7:1249-1255)，菜豆蛋白(豆子叶)(Segupta-Gopalan、C等人(1985)Proc.Natl.Acad.Sci.82:3320-3324)，植物血凝素(豆子叶)(Voelker，T等人(1987)EMBO J.6:3571-3577)，B-伴大豆球蛋白和大豆球蛋白(大豆子叶)(Chen，Z-L等(1988)EMBO J.7:297-302)，谷蛋白(水稻胚乳)，大麦醇溶蛋白(大麦胚乳)(Marris，C.等人(1988)Plant Mol.Biol.10:359-366)，谷蛋白和麦醇溶蛋白(小麦胚乳)(Colot，V等(1987)EMBO J.6:3559-3564)。与嵌合基因结构中的异源编码区可操作地连接的种子特异性基因的启动子，维持它们在转基因植物中时间和空间的表达模式。这些实例包括拟南芥2S种子贮藏蛋白基因启动子在拟南芥和甘蓝型油菜种子中表达的脑啡肽(Vanderkerckhove等人(1989)Bio/Technology 7:L929-932)，豆凝集素和豆β-菜豆蛋白启动子表达荧光素酶(Riggs等(1989)Plant Sci.63:47-57)，和小麦谷蛋白启动子表达氯霉素乙酰转移酶(Colot等(1987)EMBO J 6:3559-3564)。

诱导启动子选择性表达一种可操作地连接DNA序列对存在的内源性或外源性刺激进行响应，例如对化合物(化学诱导剂)或响应环境的、激素的、化学的和/或生育的信号。诱导性或调控型启动子包括受到如光、热、胁迫、洪水或干旱、植物激素、伤口或化学物质(乙醇，茉莉酮酸盐，水杨酸或安全剂等)调控的启动子。

同样值得考虑的还有包含有一个或多个异源调控元件组合的人工合成启动子。

本发明中的重组DNA构建体启动子可以是本领域技术人员所知的任何类型或类别的启动子，使得许多启动子中的任何一个都可以用于表达本文公开的各种多核苷酸序列，包括目的多核苷酸序列的天然启动子。用于本发明的重组DNA构建体的启动子可以基于期望结构进行挑选。

本文中所述重组DNA构建体还可以包括其它调控元件，所述调控元件包括但不限于翻译前导序列、内含子和聚腺苷酸识别序列。在某些实例中，一个重组DNA构建体还包含有一个增强子或沉默子。

一个内含序列能够被添加在5'非翻译区，所述蛋白编辑区或3'非翻译区，进而增加成熟信息的数量并积累在细胞质中。在植物和动物表达结构的转录单元中加入可剪接的内含子已被证明在mRNA和蛋白质水平上增加基因表达可达1000倍(Buchman和Berg.(1988)Mol.Cell Biol.8:4395-4405；Callis等人(1987)Genes Dev.1:1183-1200)。

植物和植物细胞

提供了一种植物、植物细胞、植物组织、种子和籽粒，在其基因组中包含有本文描述的任一重组DNA构建体，可使所述植物、植物细胞、植物组织、种子和/或籽粒编码所述多肽的表达量增加。

提供了一种植物、植物细胞、植物组织、种子和籽粒，在其基因位点上含有一种引入的可编码一个多肽的基因修饰，所述多肽含有一个氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21或24这些氨基酸序列有至少80％的一致性。在某些实例中，所述基因修饰能增加所述编码多肽的活性。在某些实例中，所述基因修饰能增加所述编码多肽的水平。在某些实例中，所述基因修饰能同时增加所述编码多肽的活性和水平。

所述植物可以是一个单子叶或双子叶植物，例如，水稻或玉米或大豆植物，比如玉米杂交或自交植物。所述植物还可以是向日葵、高粱、油菜、小麦、苜蓿、棉花、大麦、粟、甘蔗或柳枝稷。

在某些实例中，所述植物和对照植物相比较时表现出增加的耐旱性。在某些实例中，所述植物和对照植物相比较时便显出至少一种农艺性状的改变。

本领域普通技术人员熟悉用于模拟干旱条件和评估已经经受模拟或自然发生的干旱条件的植物的耐旱性的方案。例如，人们可以通过给予植物比通常需要的水少或一段时间内没有水来模拟干旱条件，并且可以通过寻找生理和/或物理条件的差异来评估耐旱性，包括(但不限于)活力，生长，大小或根长，或特别是，叶色或叶面积大小。评估耐旱性的其他技术包括测量叶绿素荧光，光合速率和气体交换速率。

与其它目的性状的堆叠

在某些实例中，本文公开的所述创造性的多核苷酸被设计成分子堆栈。这样，本文中所述的各种宿主细胞、植物、植物细胞、植物组织、种子和/或籽粒能进一步包含一个或多个目的性状。在某些实施例中，所述宿主细胞、植物、植物组织、种子和/或籽粒由一些目的多核苷酸序列的集合进行堆叠，进而创造出有多个目的性状集合的植物。就像在本文中所使用的，属于“堆叠”是指在同一植物或目的生物体中存在多种性状。例如，“性状堆叠”可以包括分子堆叠，其中序列在物理上彼此相邻。在本公开中，性状是指来源于一种特定序列或序列组的表型。在某些实例中，所述分子堆叠包括至少一个能被赋予草甘膦耐性的多核苷酸。能够赋予草甘膦耐性的多核苷酸是本领域已知的。

在某些实例中，所述分子堆叠包含有至少一个能够赋予草甘膦耐性的多核苷酸和至少一个补充的能赋予另一种除草剂耐性的多核苷酸。

在某些实例中，有本发明的多核苷酸序列的植物、植物细胞、种子和/或籽粒可与一个或多个序列堆叠，这些序列如：肌萎缩侧索硬化抑制剂、HPPD抑制剂、2,4-D、其他苯氧基生长素除草剂、芳氧基苯氧基丙酸除草剂、麦草畏、草铵膦除草剂、针对protox酶的除草剂(也称为“protox抑制剂”)。

所述含有本发明的多核苷酸序列的植物、植物细胞、植物组织、种子和/或籽粒还可与至少一个其它特性相结合，从而制造出进一步包含各种期望性状组合的植物。例如，含有本发明的多核苷酸序列的植物、植物细胞、植物组织、种子和/或籽粒能与编码杀虫或杀虫活性的多肽的多核苷酸堆叠，或含有本发明的多核苷酸序列的植物、植物细胞、植物组织、种子和/或籽粒可与植物抗病基因结合。

这些叠加组合能通过以下方法产生，但不限于通过任何常规方法或基因转化来进行育种。如果所述堆叠序列通过基因转化进入植物，所述期望多核苷酸序列能在任意时间以任意顺序被结合。所述性状可以在共转化方案中与任何转化盒组合提供的目标多核苷酸同时引入。例如，如果两个序列将会被引入，这两个序列可以包含在单独的转化盒(trans)中，也可以包含在相同的转化盒(cis)中。序列的表达可以被同一个启动子或不同的启动子驱动。在某些实例中，期望通过引入一个转化盒能够抑制目标多核苷酸的表达。可以与其他抑制盒或过表达盒的任何组合结合，以产生植物中所需的性状组合。还要进一步认识到所述多核苷酸序列可以使用位点特异性重组系统堆叠在期望的基因位点上。参见WO99/25821、WO99/25854、WO99/25840、WO99/25855和WO99/25853，所有这些均通过引用并入本文

方法：

提供了一个增加植物耐旱性和/或提高籽粒产量的方法。在植物中包含有至少一个多核苷酸表达量的增加，所述多核苷酸编码多肽的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21或24有至少80％(比如，80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或100％)的序列一致性。

在某些实例中，所述方法包括：(a)在一个可再生植物细胞中表达一个重组DNA构建体，所述重组DNA构建体包含有一个调控元件可操作地连接编码所述多肽的多核苷酸；和(b)基因组中含有所述重组DNA构建体的植物所产生的植物。在某些实例中，所述调控元件是一个异源启动子。

在某些实例中，所述方法包括：(a)向一个可再生植物细胞的基因组位点上引入一个编码所述多肽的靶向基因修饰；和(b)所述编码多肽的水平和/或活性增加的植物所产生的植物。在某些实例中，所述靶向基因修饰的引入可使用以下基因修饰技术：多核苷酸引导的核酸内切酶、CRISPR-Cas核酸内切酶、碱基编辑脱氨酶、锌指核酸酶、转录激活物样效应器核酸酶(TALEN)、工程化位点特异性巨核酸酶或Argonaute。在某些实例中，所述靶向基因修饰存在于(a)编码区；(b)非编码区；(c)调控序列；(d)非翻译区；或(e)基因组任何(a)-(d)的组合。所述靶向基因修饰编码的多肽包含的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21或24这些氨基酸序列中的任一种有至少80％的一致性。

在某些实例中，所述DNA修饰是在基因位点上的一个或多个核苷酸的插入(最好是连续的)。例如，插入一个表达调制元件(EME)，如PCT/US2018/025446所述的，与该基因进行可操作地连锁。在某些实例中，所述靶向DNA修饰可以是内源性多肽启动子替换为本领域内已知的具有较高表达的其它启动子。在某些实例中，所述靶向DNA修饰可以是本领域内已知的具有较高表达的启动子插入到5’UTR，这样内源性多肽的表达由插入启动子控制。在某些实例中，多数DNA修饰是一种多核苷酸修饰或SNP，位于调节表达蛋白稳定性的位点上。

使用本文方法的植物可以是本文描述的任何植物。在某些实例中，所述植物是玉米、大豆或水稻。

向植物、植物细胞、植物组织、种子和/或籽粒中引入期望序列可以使用多种方法。“引入”意指使序列进入植物细胞内部的方式向植物、植物细胞、种子和/或籽粒赋予本发明中的多核苷酸或产生的多肽。本文中所述方法不依赖于向植物、植物细胞、种子和/或籽粒引入一个序列这一特定方法，只需要所述多核苷酸或多肽进入植物的至少一个细胞内部。

转化方案以及将多肽或多核苷酸序列引入植物的方案可以根据靶向转化的植物或植物细胞(即单子叶植物或双子叶植物)的类型而变化。将多肽和多核苷酸引入植物细胞的合适方法包括显微注射(Crossway等人(1986)Biotechniques 4:320-334)，电穿孔(Riggs等人(1986)Proc.Natl.Acad.Sci.USA 83:5602-5606，农杆菌介导的转化(美国专利号5563055和美国专利号5981840)，直接基因转移(Paszkowski等人(1984)EMBO J.3:2717-2722)和弹道粒子加速度(例如，参见美国专利4945050、美国专利5879918、美国专利5886244和5932782；Tomes等人(1995)在植物细胞，组织和器官培养中的应用：基本方法.编辑Gamborg和Phillips(Springer-Verlag，Berlin)；McCabe等(1988)Biotechnology 6:923-926)；和Lec1转化(WO 00/28058)。另见Weissinger等(1988)Ann.Rev.Genet.22:421-477；Sanford等(1987)颗粒科学与技术5:27-37(洋葱)；Christou等(1988)植物生理学87:671-674(大豆)；McCabe等(1988)Bio/Technology 6:923-926(大豆)；Finer和McMullen(1991)In Vitro Cell Dev.Biol.27P:175-182(大豆)；Singh等人(1998)Theor.Appl.Genet.96:319-324(大豆)；Datta等人(1990)Biotechnology 8:736-740(水稻)；Klein等人(1988)Proc.Natl.Acad.Sci.USA 85:4305-4309(玉米)；Klein等人1988)Biotechnology 6:559-563(玉米)；美国专利号5240855、5322783、和5324646；Klein等(1988)Plant Physiol.91:440-444(玉米)；Fromm等(1990)Biotechnology 8:833-839(玉米)；Hooykaas Van Slogteren等(1984)Nature(London)311:763-764；美国专利号5736369(谷物)；Bytebier等(1987)Proc.Natl.Acad.Sci.USA 84:5345-5349(百合科)；De Wet等人(1985)在胚珠组织的实验操作中，ed.Chapman等人(Longman，New York)，197-209(花粉)；Kaeppler等人(1990)Plant Cell Reports 9:415-418和Kaeppler等人(1992)Theor.Appl.Genet.84:560-566(晶须介导的转化)；D'Halluin等人(1992)Plant Cell 4:1495-1505(电穿孔)；Li等(1993)Plant Cell Reports 12:250-255和Christou and Ford(1995)Annals of Botany 75:407-413(水稻)；Osjoda等(1996)Nature Biotechnology14:745-750(玉米通过根癌土壤杆菌)；所有这些都通过引用并入本文。

在另一些实例中，本文所述的多核苷酸可以通过病毒或病毒核酸的侵染被引入植物中。通常，这些方法主要是将本文中的核苷酸构建体导入一个DNA或RNA分子中。应该认识到，本发明所述的多核苷酸序列可以被初步合成作为病毒多蛋白的一部分，之后可以在体内或体外通过蛋白质水解处理以产生所需的重组蛋白。此外，本发明所述启动子也包含利用病毒RNA聚合酶进行转录的启动子。向植物引入多核苷酸并表达其编码的涉及DNA或RNA分子的蛋白质的方法是本领域内已知的。例如，见美国专利号5889191、5889190、5866785、5589367、5316931和Porta等，(1996)Molecular Biotechnology 5:209-221，本文通过引用并入。

所述已经转化的细胞可以按照常规方法生长成植物。例如，见McCormick等(1986)Plant Cell Reports 5:81-84.然后，这些植物可以生长，用相同的转化品系或不同的品系进行授粉，并鉴定获得的后代具有所需表型特征的组成型表达。种植两代或更多的子代，以确保所需表型特征的表达能稳定保存和集成，然后收获的种子同样确保所需表型特征的表达能被获得。以这种方式，本文提供了含有本发明的多核苷酸的转化种子(也称为“转基因种子”)，例如，作为表达和的一部分，稳定整合到它们的基因中。

包括前文讨论的通过植物转化技术衍生的转化植物细胞，可以培养再生成一整个拥有转化表型(如，本发明的多核苷酸)的植株，以及因此获得期望的表型，例如增加的产量。对于玉米的转化和再生，参见Gordon Kamm等，The Plant Cell，2:603-618(1990)。

多种方法可以用于在基因位点引入一个基因修饰，进而可以在植物、植物组织、植物细胞、种子和/或籽粒中编码本文所述的多肽。在某些实例中，所述靶向DNA修饰通过以下基因修饰技术：多核苷酸引导的内切核酸酶，CRISPR-Cas内切核酸酶，碱基编辑脱氨酶，锌指核酸酶，转录激活物样效应核酸酶(TALEN)，工程位点特异性大范围核酸酶，或Argonaute。

在某些实施例中，可以通过在基因组中靠近所需改变的限定位置诱导双链断裂(DSB)或单链断裂来促进所述的基因组修饰。可以使用任何可用的DSB诱导剂诱导DSB，包括但不限于TALEN，大范围核酸酶，锌指核酸酶，Cas9 gRNA系统(基于细菌CRISPR-Cas系统)，引导的cpf1内切核酸酶系统等。在一些实施方案中，DSB的引入可以与引入多核苷酸修饰模板组合。

一个多核苷酸修饰模板可以通过本领域内已知的任何方法被引入细胞中，包括但不限于，瞬时引入方法，转染，电穿孔，显微注射，颗粒介导的递送，局部应用，晶须介导的递送，通过细胞穿透肽的递送或介孔二氧化硅纳米颗粒(MSN)介导的直接递送。

所述多核苷酸修饰模板可以作为一个单链多核苷酸分子、一个双链多核苷酸分子或作为环状DNA(载体DNA)的一部分被引入细胞。所述多核苷酸修饰模板也可以被链接在向导RNA和/或Cas核酸内切酶上。

一个“修饰的核苷酸”或“编辑核苷酸”是指期望的核苷酸序列当和它的非修饰性核苷酸序列比较时，包含有至少一个改变。这种“改变”包括如：(i)至少一个核苷酸的替换；(ii)至少一个核苷酸的删除；(iii)至少一个核苷酸的插入，或(iiii)任意(i)-(iii)的组合。

术语“多核苷酸修饰模板”包括，当和被编辑的核苷酸序列比较时，含有至少一个核苷酸修饰的多核苷酸。一个核苷酸修饰可以是至少一个核苷酸的替换、添加或删除。所述多核苷酸修饰模板可以进一步包含有内源核苷酸序列可选择地在至少一个核苷酸修饰的侧边，其中所述侧翼内源核苷酸序列提供充足的同源基因用于编辑所需核苷酸。

编辑结合DSB和修饰模板的基因组序列的流程通常包括：提供一个宿主细胞，一个DSB诱导剂，或一个编码DSB诱导剂的核酸，能够识别在染色体序列中的目标序列并能够诱导基因组序列中的DSB，以及当与被编辑的核苷酸序列相比较时，多核苷酸修饰模板包含有至少一个核苷酸的改变。所述多核苷酸修饰模板进一步包含连接在至少一个核苷酸改变侧翼的核苷酸序列，所述侧翼连接序列可在DSB相对应的染色体区域互相替换。

可以通过本领域内已知的任何方法,给细胞提供所述核酸内切酶，包括但不限于瞬时导入方法、转染、显微注射和/或局部应用或间接通过重组构建体。所述核酸内切酶可以作为蛋白质或作为引导多核苷酸复合物直接提供给细胞，或间接通过重组构建。所述核酸内切酶可以瞬时被引入一个细胞，或使用领域内已知的任何方法整合进入宿主细胞的基因组内。在CRISPR-Cas系统的情况下，如2016年5月12日公布的WO2016073433中所述，可以用细胞穿透肽(CPP)促进内切核酸酶和/或引导的多核苷酸摄入细胞。

除了通过双链断裂技术进行修饰之外，使用碱基编辑技术实现对没有这种双链断流的一个或多个碱基的修饰，如Gaudelli等(2017)可编程碱基编辑基因DNA中的A*T至G*C而没有DNA切割。Nature 551(7681):464-471；Komor等(2016)可编程编辑基因组DNA靶碱基而无双链DNA切割，Nature 533(7603):420-425。

这些包含有dCas9切口酶和合适的脱氨酶的融合，且它们可以转换如，胞嘧啶转化为尿嘧啶而不诱导靶DNA的双键断裂。尿嘧啶通过DNA复制或修复随后转换为胸腺嘧啶。具有目标灵活性和特异性的改良碱基编辑器用于编辑内源基因座以产生有目标变异并提高谷物籽粒产量。同样的，腺嘌呤碱基编辑器能使腺嘌呤向肌苷转变，然后通过复制或修复转化为鸟嘌呤。这样，目标碱基的转化，如在使用适当的特定于站点的基本编辑器制作的另一个位置中C·G向T·A转化和T·A向C·G转化。

在一个实例中，碱基编辑是一种能够直接将目标基因座上一个碱基对转化为另一个碱基对而不需要双链DNA断裂(DSB)、同源性定向修复(HDR)过程、或外部供体DNA模板的基因组编辑方法。在实例中，碱基编辑包括(i)一个催化受损的CRISPR-Cas9突变体的突变使得它们的一个核酸酶结构域不能产生DSBs；(ii)一个单链特异性胞苷/腺嘌呤脱氨酶，可以在Cas9产生的单链DNA气泡中的适当的核苷酸窗口内将转化C为U或A为G；(iii)阻碍尿嘧啶切除的尿嘧啶糖基化酶抑制剂(UGI)和降低碱基编辑效率和产物纯度的下游过程；和(iv)切口酶活性切割未编辑的DNA链，然后进行细胞DNA修复过程以替换含G的DNA链。

如本发明所述，“基因组区域”是细胞基因组中染色体的一段，出现在靶位点的任一侧，或者还包含靶位点的一部分。所述基因组区域可以包括至少5-10,5-15,5-20,5-25,5-30,5-35,5-40,5-45,5-50,5-55,5-60,5-65,5-70,5-75,5-80,5-85,5-90,5-95,5-100,5-200,5-300,5-400,5-500,5-600,5-700,5-800,5-900,5-1000,5-1100,5-1200,5-1300,5-1400,5-1500,5-1600,5-1700,5-1800,5-1900,5-2000,5-2100,5-2200,5-2300,5-2400,5-2500,5-2600,5-2700,5-2800.5-2900,5-3000,5-3100或更多碱基，使得基因组区域具有足够的同源性以与相应的同源性区域进行同源重组。

TAL效应核酸酶(TALEN)是一类序列特异性核酸酶，可用于在植物或其他生物基因组的特定靶序列上产生双链断裂(Miller等(2011)Nature Biotechnology 29:143-148)。

内切核酸酶是切割多核苷酸链内的磷酸二酯键的酶。核酸内切酶包括在特定位点切割DNA而不破坏碱基的限制性内切酶，以及与限制性内切酶一样在特定识别位点结合和切割的大范围核酸酶(也称为归巢核酸内切酶(HEase))，但是大范围核酸酶的识别位点通常更长，约18bp或更多(专利申请PCT/US12/30061，于2012年3月22日提交)。大范围核酸酶根据保守序列基序分为四个家族，这些家族是LAGLIDADG，GIY-YIG，H-N-H和His-Cys-box家族，这些基序参与金属离子的配位和磷酸二酯键的水解。酶因其长识别位点和耐受某些序列而著称大范围核酸酶的命名约定与其他限制性核酸内切酶的命名约定相似，大范围核酸酶的特征还在于前缀F-，I-或PI-，分别由独立的ORF，内含子和内含子编码。重组过程的一步涉及多核苷酸在识别位点处或附近切割。切割活性可用于产生双链断裂。对于位点特异性重组酶及其识别位点的综述，参见Sauer(1994)Curr Op Biotechnol 5:521-7；和Sadowski(1993)FASEB 7:760-7。在一些实例中，重组酶来自整合酶或分解酶家族。

锌指核酸酶(ZFNs)是由锌指DNA结合结构域和双链断裂诱导剂结构域组成的工程化双链断裂诱导剂。识别位点特异性由锌指结构域赋予，其通常包含两个，三个或四个锌指，例如具有C2H2结构，然而其他锌指结构锌指结构域适用于设计特异性结合所选多核苷酸识别序列的多肽.ZFN包括与非特异性内切核酸酶结构域连接的工程化DNA结合锌指结构域，例如来自类型的核酸酶结构域IIs内切核酸酶，如FokI.Addi功能性功能可以与锌指结合结构域融合，包括转录激活因子结构域，转录抑制因子结构域和甲基化酶。在一些实例中，核酸酶结构域的二聚化是切割活性所必需的。每个锌指识别三个连续的碱基对。目标DNA。例如，3指结构域识别9个连续核苷酸的序列，具有核酸酶的二聚化要求，使用两组锌指三联体结合18个核苷酸的识别序列。

使用DSB诱导剂(例如Cas9-gRNA复合物)的基因组编辑，例如在2015年3月19日发布的美国专利申请US 2015-0082478 A1，2015年2月26日发布的WO2015/026886 A1，2016年1月14日发布的WO2016007347和2016年2月18日发布的WO201625131，所有这些都是合并的在本发明中作为参考。

示例

以下是本发明某些方面的具体实施例的实例。这些实施例仅用于说明目的，并不以任何方式限制本发明的范围。

示例1

耐旱基因的克隆和载体构建

使用含有来自花椰菜花叶病毒35S(CaMV 35S)启动子的四种多聚化增强子元件的双元构建体，并且水稻激活标签群体(ATLs)由四种粳稻(Oryza sativa ssp.japonica)品种(中华11号，潮友1号，台中65号和日本晴)，如Lin和Zhang((2005)Plant Cell Rep.23:540-547)所述，通过农杆菌介导的转化方法转化。开发产生的转基因品系并收获转基因种子以形成水稻活化标记群体。

在重复的田间试验中证实耐旱标签系(ATLs)并测定它们的T-DNA插入基因位点。克隆T-DNA左边界和右边界附近的基因，并通过田间筛选重现功能基因。本文仅显示了概括的功能基因。并基于表2中所示这些基因的LOC ID，设计引物用于克隆水稻抗旱基因OsDN-DTP8，OsKIK1，OsRWDD1，OsUFD1，OsPPR2，OsSAUR28-1，OsHYS1，OsFBID1。

表2.水稻基因名称,基因IDs(来自TIGR)和构建体IDs

琼脂糖凝胶电泳后，使用柱试剂盒提取PCR扩增产物，然后与TA克隆载体连接。通过测序确认这些构建体中的序列和方向。将每个基因克隆到植物双元构建体中。

示例2

转基因水稻品系的转化和基因表达分析

如Lin和Zhang((2005)Plant Cell Rep.23:540-547)所述，通过农杆菌介导的转化，用实施例1中制备的载体或空载体(DP0158)转化中华11号(Oryza sativa L.)。将转化实验室产生的转基因幼苗(T0)移植到田间以获得T1种子。筛选T1和随后的T2种子以确认转化，并将阳性鉴定的转基因种子用于以下性状筛选。

通过RT-PCR测定转基因水稻植物叶中的基因表达水平。设计引物用于过表达转基因水稻中OsDN-DTP8，OsRWDD1，OsUFD1，OsSAUR28-1和OsHYS1基因的RT-PCR。将ZH11-TC(组织培养的ZH11水稻)中的表达水平设定为1.00，并将DP1130，DP0828，DP0903和DP1121转基因水稻植物中的表达水平与ZH11-TC进行比较。将DP0158中的表达水平设定为1.00，并将DP0984转基因水稻植物中的表达水平与DP0158进行比较。基于EF-1αmRNA水平对基因表达进行标准化，并且基因表达分析的结果在下表3中提供。

表3.转基因水稻植株相对表达水平的增长倍数

示例2

转基因水稻植株的性状

测试来自实施例2的转基因水稻植物和ZH11-TC和DP0158水稻植物的：(a)耐旱性，(b)百草枯耐受性。

将来自实施例2的植物的T2种子在32℃下用800ppm多菌唑灭菌8小时并洗涤3-5次，在32℃下在水中浸泡16小时，并在35-37℃下发芽18小时。在孵化器里。发芽种子用于每个试验如下：

耐旱性-将发芽种子种植在苗床田中。在3叶期，将幼苗移植到试验田中，每个转基因品系重复4次，每次重复10株，并将4个重复种植在同一块中。ZH11-TC和DP0158幼苗在同一区块的转基因品系附近，并在统计分析中用作对照。水稻植物通过常规做法使用杀虫剂和肥料进行管理。在穗开始阶段停止浇水，以便根据天气条件(温度和湿度)在开花阶段给予干旱胁迫。使用TDR30(Spectrum Technologies，Inc)以每块约10个位点每4天测量土壤水含量。在实验期间观察并记录植物表型。表型包括抽穗期，卷叶度，干旱敏感性和耐旱性。中午特别注意卷叶度。在生长季节结束时，从每行的中间收获每个转基因品系的6个代表性植物，并测量每株植物的谷粒产量。使用混合线性模型对谷物产量数据进行统计分析。

百草枯耐受性-将发芽种子置于底部有孔的管中，在30℃下培养5天直至一片叶和一个末端芽期。选择约3.5～4cm高的均匀幼苗进行百草枯试验。在该实验中使用随机区组设计。有五个区块，每个区块有16x12个孔。将每个转基因品系置于一行(12株植物/系)中，并将ZH11-TC和DP0158幼苗随机分成3行(3×12株植物)一块。然后将幼苗用0.8μM百草枯溶液在10小时/14小时夜间处理7天，处理的幼苗首先遇到黑暗并吸收每两天更换一次的百草枯溶液。处理7天后，计数绿色幼苗。那些整体保持绿色而不受损害的幼苗被认为是百草枯耐受的幼苗；那些漂白的叶子或茎不被认为是百草枯耐受的幼苗。

耐受率用作该性状筛选的参数，其是保持绿色并且在总植物数量上显示耐受表型的植物的百分比。使用

“rep”的随机效应，

的统计方法的统计模型分析数据。

研究结果见表4，表中提供了每种构建体的转基因品系的组合数据。

表4.转基因水稻植株的农艺性状

DP1130转基因水稻植物在三年内在海南田进行了三次测试。结果表明，与对照相比，大田干旱条件下DP1130转基因水稻单株平均产量增加。如表4所示，测试了12个株系，3个株系在海南田间观察到良好的结实率。8个品系的产量显著高于DP0158对照(P<0.1)，6个株系的产量明显高于ZH11-TC对照。这12个株系的单株平均产量分别比ZH11-TC和DP0158对照高33％和54％。这些数据表明OsDN-DTP8是一种水稻抗旱基因。

DP0808转基因水稻植株两年内在海南进行了两次测试。结果表明，与对照相比，大田干旱条件下DP0808转基因水稻单株平均产量增加。如表4所示，在海南田测试了6个株系。4个株系的产量显著高于DP0158和ZH11-TC对照(P<0.1)。这6个株系的单株平均产量分别比ZH11-TC和DP0158对照高73％和113％。这些数据表明OsKIK1是一种水稻抗旱基因。

DP0828转基因水稻植株三年内在宁夏和海南进行了三次测试。所有这些都表明，与对照相比，DP0828转基因水稻在田间干旱条件下的单株平均产量增加。如表4所示，在海南田测试了12个株系。8个株系的产量显著高于DP0158对照(P<0.1)，5个株系的产量明显高于ZH11-TC对照。这12个株系的单株平均产量分别比ZH11-TC和DP0158对照高52％和68％。DP0828转基因水稻植物也在百草枯测定中测试两次。获得了一致的结果。在第二个实验中，在构建体水平上，所有DP0828转基因株系的百草枯平均耐受率(80％)明显高于ZH11-TC(63％)和DP0158(66％)对照。在转基因株系水平上，7个OsRWDD1转基因品系具有比ZH11-TC对照明显更高的耐受率，并且6个株系具有比DP0158对照明显更高的耐受率(表4)。这些结果表明，与两种对照相比，OsRWDD1转基因水稻植物具有增强的耐旱性和百草枯耐受性。OsRWDD1在增强转基因植物的耐旱性和百草枯耐受性或抗氧化能力方面起作用。

DP0903转基因水稻植株两年内在宁夏和海南进行了三次测试。其中两次表明，与对照相比，DP0903转基因水稻在田间干旱条件下单株平均产量增加。如表4所示，在海南地区测试了8个株系。8个株系的产量显著高于DP0158对照(P<0.1)，4个株系的产量明显高于ZH11-TC对照。这8个株系的单株平均产量分别比ZH11-TC和DP0158对照高30％和61％。这些结果表明OsUFD1转基因水稻植株具有增强的耐旱性。

DP0839转基因水稻植株三年内在宁夏和海南进行了三次测试。其中两次表明，与对照相比，DP0839转基因水稻在田间干旱条件下单株平均产量增加。如表4所示，在海南田测试了12个株系。6个株系的产量显著高于DP0158对照(P<0.1)，7个株系的产量明显高于ZH11-TC对照。这12个株系的单株平均产量分别比ZH11-TC和DP0158对照高87％和46％。DP0839转基因水稻植物也在百草枯测定中测试两次。获得了一致的结果。在第一个实验中，在构建体水平上，所有DP0839转基因株系的平均百草枯耐受率(82％)明显高于ZH11-TC(64％)和DP0158(63％)对照。在转基因株系水平上，6个OsPPR2转基因株系具有比ZH11-TC和DP0158对照明显更高的耐受率(表4)。这些结果表明，与两种对照相比，OsPPR2转基因水稻植物具有增强的耐旱性和百草枯耐受性。OsPPR2在增强转基因植物的耐旱性和百草枯耐受性或抗氧化能力方面起作用。

DP1122转基因水稻植株两年内在宁夏和海南进行了三次测试。所有这些都表明，与对照相比，DP1122转基因水稻在田间干旱条件下单株平均产量增加。如表4所示，在海南田测试了7个株系。4个株系的产量显著高于DP0158和ZH11-TC对照(P<0.1)，并且在田间观察到良好的结实率。这7个株系的单株平均产量分别比ZH11-TC和DP0158对照高33％和51％。DP1122转基因水稻植株也在百草枯测定中测试两次，获得了一致的结果。在第一次实验中，在构建体水平上，9个DP1122转基因株系(44％)的百草枯平均耐受率明显高于ZH11-TC(31％)，并且高于DP0158(39％)。在转基因株系水平上，9个OsSAUR28-1转基因株系中的3个具有比ZH11-TC和DP0158对照明显更高的耐受率(表4)。这些结果表明，与两种对照相比，OsSAUR28-1转基因水稻植株具有增强的耐旱性和百草枯耐受性。OsSAUR28-1在增强转基因植物的耐旱性和百草枯耐受性或抗氧化能力方面起作用。

DP0984转基因水稻植株三年内在宁夏和海南进行了三次测试。其中两次表明，与对照相比，DP0984转基因水稻在田间干旱条件下单株平均产量增加。如表4所示，测试了12个株系，其中5个在海南田观察到良好的结实率。10个株系的产量显著高于DP0158(P<0.1)，9个株系的产量明显高于ZH11-TC。这12个株系的单株平均产量分别比ZH11-TC和DP0158对照高44％和59％。这些结果表明OsHYS1转基因水稻植物具有增强的耐旱性。

DP0893转基因水稻植株三年内在宁夏和海南进行了三次测试。其中两次表明，与对照相比，DP0893转基因水稻在田间干旱条件下单株平均产量增加。如表4所示，在海南田测试了6个株系。6个株系的产量显著高于DP0158(P<0.1)，5个株系的产量明显高于ZH11-TC。这6个株系的单株平均产量分别比ZH11-TC和DP0158对照高55％和85％。这些结果表明OsFBID1转基因水稻植株具有增强的耐旱性。

总之，这些结果表明，与对照植物相比，OsDN-DTP8，OsKIK1，OsRWDD1，OsUFD1，OsPPR2，OsSAUR28-1，OsHYS1和OsFBID1转基因水稻植株对干旱条件的耐受性增加。

示例4

具有水稻耐旱性基因的玉米的转化和评估

玉米植株将用编码本文所述多肽的多核苷酸之一或来自玉米、拟南芥或其他物种的相应同源基因转化。该基因在玉米转化载体中的表达可以在组成型启动子的控制下，例如玉米遍在蛋白启动子(Christensen等人(1989)Plant Mol.Biol.12:619-632和Christensen等人(1992)Plant Mol.Biol.18:675-689)或在另一个启动子的控制下，例如应激反应性启动子或组织优选的启动子。基本上如国际专利公开WO 2009/006276中所述，可以通过粒子轰击将重组DNA构建体引入玉米细胞中。或者，基于Zhao等人在Meth中所述，通过农杆菌介导的转化，用重组DNA构建体转化玉米植物。Mol.Biol。318:315-323(2006)和Zhao等人，Mol.Breed。8：323-333(2001)和1999年11月9日发布的美国专利号5981840。

再生植物的后代，例如T1植物，可以经受基于土壤的干旱胁迫。使用图像分析，可以在干旱胁迫之前和期间多次测量植物面积、体积、生长速率和颜色。相对于对照，干旱胁迫期间叶片枯萎或叶面积减少、黄色积累减少和/或生长速率增加的显著延迟将被认为是该基因在玉米中起作用以增强耐旱性的证据。

示例5

拟南芥水稻抗旱基因的实验室干旱筛选

为了了解水稻耐旱基因是否能提高双子叶植物的耐旱性或其他性状，本文所述的水稻表达载体可通过农杆菌介导的转化程序，使用花浸法转化到拟南芥(哥伦比亚)，并鉴定转基因植物(Clough，S.T.和Bent，A.F。(1998)《植物杂志》16735–743；张，X.等人(2006)自然协议1:641-646)。

再生植株的后代，如T1植株，可能受到基于土壤的干旱胁迫。通过图像分析，可以在干旱胁迫之前和期间多次测量植物的面积、体积、生长速率和颜色。干旱胁迫期间，与对照相比，萎蔫或叶面积减少的显著延迟、黄色积累的减少和/或生长速率的增加将被视为双子叶植物中基因功能增强抗旱性的证据。

序列表

<110> 未名生物农业集团有限公司

先锋海外公司

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agagatggtg gcggcgtcga cgcggcggac acgctgtcgc aggggcagtc gctgggcgcg 120

aacgacatgc tggtgtcggc gaacggcacg ttcaaggtgg ggttcttcac gccggcgggt 180

ggcgacccgg ggaaggtgta cctcggggtg atgtacgcga cgtcgaacgt gcagacggtg 240

atgtgggtgg cgaaccgcga tgcccccgtg agaacggcgg cgggggccgc ctccgccacc 300

gtgacgggct ccggcgagct gctggtgaag gagggcgacc gcgtcgcgtg gcggacgaac 360

gcgtcggccg cggggcgctc caaacacacg ctgacgatcc gcgacgacgg gaacctcgtg 420

atctccggca gcgacgccgc cggcacggac gtagagtggg agagcttcca ccacccgacg 480

gacaccttcg tcccgggcat ggagatcgcg ctccggcaga ccaacggcga ccgcacgctg 540

tacacgtcgt ggaggagcga cgccgacccg gccaccggcg acttcacgct ggggctggac 600

gcgtcggcgc agctctacat ctggcggagc cagggcggca agaactcaac ctactggagg 660

tcggggcagt gggcgagcgg caacttcgtc ggcatcccgt ggcgggcgct ctacgtctac 720

ggcttcaagt tgaacggcga cccgccgccg atcgccggcg acatgtccat cgcgttcacc 780

cccttcaact cgtcgctcta ccgcttcgtg ctccggccga acggcgtcga gacgtgctac 840

atgctcctcg gctccggcga ctgggagctc gtctggtcgc agccgaccat cccctgccac 900

cgctacaact tgtgcggcga caacgccgag tgcaccgccg acgacaacga gcccatctgc 960

acctgcttca caggttttga gccaaaatct ccgcaagagt acaacaacgg caactggacg 1020

cagggctgcg tgaggagcgt tccactgacg tgcagcagcg agaggaacaa cacgaccgcc 1080

ggtggcgccg gcgccggtgg cggcgacggg ttcaccgtca tccggggcgt gaagctgccg 1140

gacttcgccg tgtggggatc gctggtgggc gacgcgaact cgtgcgagaa ggcgtgcctg 1200

ggcaactgct catgcggggc gtacagctac agcaccggca gctgcctcac ctgggggcag 1260

gagctggtgg acatcttcca gttccaaacc ggcaccgagg gagcgaaata cgacctctac 1320

gtcaaggtcc catcttctct attagataaa agctcggggc gatggaaaac cgttgtcgtc 1380

gtcgtagttg tggtcgtggt ggttgtattg ctggcatcag gccttctcat gtggaagtgc 1440

aggagacgaa ttaaagagaa acttggcatt ggtaggaaaa aggcacaact cccgttgctg 1500

cgtcctgcga gggatgcaaa gcaggatttc tcagggccag cgcaatctga acacgagaaa 1560

tcagaggagg gcaagaactg cgagctgccg ctgttcgcgt tcgagacctt agcgacggcc 1620

accgacaact tcagcatctc gaacaagctc ggagagggag gcttcggcca tgtctacaag 1680

ggaaggctcc ctggaggaga agagatcgcg gtgaagaggc tgtcccggag ctccgggcag 1740

gggctggagg agttcaagaa cgaggtgatc ctgatcgcga agctgcagca ccgcaatctt 1800

gtcaggttgc tgggatgctg catccagggc gaggagaaga tcctggtgta cgaatacatg 1860

cccaacaaga gcctcgacgc cttcctcttc gatccggaga gaagagggct cctggactgg 1920

aggacgaggt tccagatcat cgaaggcgtg gcgcgagggc tcctgtacct ccaccgcgac 1980

tcgaggctcc gcgtggtgca ccgcgacctc aaggccagca acatcctcct cgaccgcgac 2040

atgaacccca agatctccga cttcggcatg gccaggatct tcggcggcga ccagaaccag 2100

gtcaacacca accgcgtcgt cggcacactg ggctacatgt caccggagta cgcgatggag 2160

gggctcttct cggtgaggtc ggacgtgtac agcttcggca tcctcatcct ggagatcatc 2220

acgggccaga agaacagcag cttccaccac atggagggct ccctcaacat cgtcggctac 2280

gcgtggcagc tgtggaacgg ggacagaggg caggagctga tcgacccggc gatccggggg 2340

acgtgccccg cgaaggaggc gctgcggtgc gtgcacatgg cgctgctgtg cgtgcaggac 2400

cacgcgcacg accgcccgga catcccctac gtggtgctca cgctcggcag cgactcctcc 2460

gtgctgccca cgccgcgccc gccgacgttc acgctgcagt gcacgtcgtc gtcgtcgggg 2520

cgggacatgt actacaggga caaggaggag tcctactccg ccaacgacct caccgtcacc 2580

atgctccaag gcaggtag 2598

<210> 6

<211> 865

<212> PRT

<213> Oryza sativa

<400> 6

Met Ala Ser Thr Ala Ala Ile Ala Ala Leu Ala Leu Val Phe Phe Val

1 5 10 15

Leu Leu Cys Val Arg Asp Gly Gly Gly Val Asp Ala Ala Asp Thr Leu

20 25 30

Ser Gln Gly Gln Ser Leu Gly Ala Asn Asp Met Leu Val Ser Ala Asn

35 40 45

Gly Thr Phe Lys Val Gly Phe Phe Thr Pro Ala Gly Gly Asp Pro Gly

50 55 60

Lys Val Tyr Leu Gly Val Met Tyr Ala Thr Ser Asn Val Gln Thr Val

65 70 75 80

Met Trp Val Ala Asn Arg Asp Ala Pro Val Arg Thr Ala Ala Gly Ala

85 90 95

Ala Ser Ala Thr Val Thr Gly Ser Gly Glu Leu Leu Val Lys Glu Gly

100 105 110

Asp Arg Val Ala Trp Arg Thr Asn Ala Ser Ala Ala Gly Arg Ser Lys

115 120 125

His Thr Leu Thr Ile Arg Asp Asp Gly Asn Leu Val Ile Ser Gly Ser

130 135 140

Asp Ala Ala Gly Thr Asp Val Glu Trp Glu Ser Phe His His Pro Thr

145 150 155 160

Asp Thr Phe Val Pro Gly Met Glu Ile Ala Leu Arg Gln Thr Asn Gly

165 170 175

Asp Arg Thr Leu Tyr Thr Ser Trp Arg Ser Asp Ala Asp Pro Ala Thr

180 185 190

Gly Asp Phe Thr Leu Gly Leu Asp Ala Ser Ala Gln Leu Tyr Ile Trp

195 200 205

Arg Ser Gln Gly Gly Lys Asn Ser Thr Tyr Trp Arg Ser Gly Gln Trp

210 215 220

Ala Ser Gly Asn Phe Val Gly Ile Pro Trp Arg Ala Leu Tyr Val Tyr

225 230 235 240

Gly Phe Lys Leu Asn Gly Asp Pro Pro Pro Ile Ala Gly Asp Met Ser

245 250 255

Ile Ala Phe Thr Pro Phe Asn Ser Ser Leu Tyr Arg Phe Val Leu Arg

260 265 270

Pro Asn Gly Val Glu Thr Cys Tyr Met Leu Leu Gly Ser Gly Asp Trp

275 280 285

Glu Leu Val Trp Ser Gln Pro Thr Ile Pro Cys His Arg Tyr Asn Leu

290 295 300

Cys Gly Asp Asn Ala Glu Cys Thr Ala Asp Asp Asn Glu Pro Ile Cys

305 310 315 320

Thr Cys Phe Thr Gly Phe Glu Pro Lys Ser Pro Gln Glu Tyr Asn Asn

325 330 335

Gly Asn Trp Thr Gln Gly Cys Val Arg Ser Val Pro Leu Thr Cys Ser

340 345 350

Ser Glu Arg Asn Asn Thr Thr Ala Gly Gly Ala Gly Ala Gly Gly Gly

355 360 365

Asp Gly Phe Thr Val Ile Arg Gly Val Lys Leu Pro Asp Phe Ala Val

370 375 380

Trp Gly Ser Leu Val Gly Asp Ala Asn Ser Cys Glu Lys Ala Cys Leu

385 390 395 400

Gly Asn Cys Ser Cys Gly Ala Tyr Ser Tyr Ser Thr Gly Ser Cys Leu

405 410 415

Thr Trp Gly Gln Glu Leu Val Asp Ile Phe Gln Phe Gln Thr Gly Thr

420 425 430

Glu Gly Ala Lys Tyr Asp Leu Tyr Val Lys Val Pro Ser Ser Leu Leu

435 440 445

Asp Lys Ser Ser Gly Arg Trp Lys Thr Val Val Val Val Val Val Val

450 455 460

Val Val Val Val Val Leu Leu Ala Ser Gly Leu Leu Met Trp Lys Cys

465 470 475 480

Arg Arg Arg Ile Lys Glu Lys Leu Gly Ile Gly Arg Lys Lys Ala Gln

485 490 495

Leu Pro Leu Leu Arg Pro Ala Arg Asp Ala Lys Gln Asp Phe Ser Gly

500 505 510

Pro Ala Gln Ser Glu His Glu Lys Ser Glu Glu Gly Lys Asn Cys Glu

515 520 525

Leu Pro Leu Phe Ala Phe Glu Thr Leu Ala Thr Ala Thr Asp Asn Phe

530 535 540

Ser Ile Ser Asn Lys Leu Gly Glu Gly Gly Phe Gly His Val Tyr Lys

545 550 555 560

Gly Arg Leu Pro Gly Gly Glu Glu Ile Ala Val Lys Arg Leu Ser Arg

565 570 575

Ser Ser Gly Gln Gly Leu Glu Glu Phe Lys Asn Glu Val Ile Leu Ile

580 585 590

Ala Lys Leu Gln His Arg Asn Leu Val Arg Leu Leu Gly Cys Cys Ile

595 600 605

Gln Gly Glu Glu Lys Ile Leu Val Tyr Glu Tyr Met Pro Asn Lys Ser

610 615 620

Leu Asp Ala Phe Leu Phe Asp Pro Glu Arg Arg Gly Leu Leu Asp Trp

625 630 635 640

Arg Thr Arg Phe Gln Ile Ile Glu Gly Val Ala Arg Gly Leu Leu Tyr

645 650 655

Leu His Arg Asp Ser Arg Leu Arg Val Val His Arg Asp Leu Lys Ala

660 665 670

Ser Asn Ile Leu Leu Asp Arg Asp Met Asn Pro Lys Ile Ser Asp Phe

675 680 685

Gly Met Ala Arg Ile Phe Gly Gly Asp Gln Asn Gln Val Asn Thr Asn

690 695 700

Arg Val Val Gly Thr Leu Gly Tyr Met Ser Pro Glu Tyr Ala Met Glu

705 710 715 720

Gly Leu Phe Ser Val Arg Ser Asp Val Tyr Ser Phe Gly Ile Leu Ile

725 730 735

Leu Glu Ile Ile Thr Gly Gln Lys Asn Ser Ser Phe His His Met Glu

740 745 750

Gly Ser Leu Asn Ile Val Gly Tyr Ala Trp Gln Leu Trp Asn Gly Asp

755 760 765

Arg Gly Gln Glu Leu Ile Asp Pro Ala Ile Arg Gly Thr Cys Pro Ala

770 775 780

Lys Glu Ala Leu Arg Cys Val His Met Ala Leu Leu Cys Val Gln Asp

785 790 795 800

His Ala His Asp Arg Pro Asp Ile Pro Tyr Val Val Leu Thr Leu Gly

805 810 815

Ser Asp Ser Ser Val Leu Pro Thr Pro Arg Pro Pro Thr Phe Thr Leu

820 825 830

Gln Cys Thr Ser Ser Ser Ser Gly Arg Asp Met Tyr Tyr Arg Asp Lys

835 840 845

Glu Glu Ser Tyr Ser Ala Asn Asp Leu Thr Val Thr Met Leu Gln Gly

850 855 860

Arg

865

<210> 7

<211> 1336

<212> DNA

<213> Oryza sativa

<400> 7

ctcgtcgcgg ccgtggaaat tgccatgatg acgccgggat cgtcctcgac ttcggtgccg 60

ggagatgagg ccgacgccgg caactgggat gcgggggtgg agacggcggc gcggctggag 120

gcgatggttc atgcggagga cgaactctcg gaggagcaga ttcaagccaa caaccagaca 180

caggaagatg agctgctggc actacaggcc atctatggtg atgatttggt tatcttcgac 240

aataaggacg gccttcgatt tttccagatt tctctgcact atcaactcgc cggtgacatc 300

cgagtttacc tgaatgtctg ccccaacggg agaacagaaa ccggagcaga aaacgacgac 360

gacgacgaca gtgaccgact cttgtatgct tgcagcctgc agcatctgcc tcctgttgtg 420

ctagcctgcc tactaccacg tttatatccg agtcaccgtg ccccctactt cgtggtcgcc 480

gcgaagtggc tggacgagcc ggaagtttca agcttctgct ctgttcttga tgagatctgg 540

gcagagcagc ctgcagggca agaggtggtg tacaaatggg tggactggct gagcacctct 600

tcctggtttt gcattgcttc agatgatcag attgtatttg gaccagatgc agactcggct 660

ggtggtgatg accgggcaat cggaagaagc tgctcccttg attctatgat ccctctaatt 720

caacgttaca gcaaggagag atcacatgaa attttcgctc gaagaatcca tgagtgcgga 780

gtctgtctca gtgaaaatac aggcagaaac ttcatacagc tcccatgcag ccactccttc 840

tgcgtcaagt gcatggagac gcagtgcagg atccacgtga aggaagggag cgtggcgagg 900

ctgacatgcc cggacacgtc gtgccgccgg ccgctgccgc cggcgctgct gaggggcctc 960

ctcggcgacg gcgagtacgc gcggtgggag tcgctggtgc tgcgaggatg ctggacacga 1020

tgcccgacgt ggcctactgc cccaggtgca gcgccgcgtg cgtggcggcc ggcgacgacg 1080

cccagtgctc gaggtgcttc ttcaccttct gcgccgtctg ccgggagcgg cgccacgtcg 1140

gggacacctg cgtctccccg aaccagatgc tcgacatcat gctggaacgg cagaaggaga 1200

agaggccgtt ggcggcgccg tcgccggaca gccaggcggt gtcgtcgaag aggaagatgg 1260

aggagcttct gagcctccgc gaggtgatgc gcacgtcgag gcagtgcccg tcgtgcaaga 1320

tggccgtgtc caagac 1336

<210> 8

<211> 1248

<212> DNA

<213> Oryza sativa

<400> 8

atgatgacgc cgggatcgtc ctcgacttcg gtgccgggag atgaggccga cgccggcaac 60

tgggatgcgg gggtggagac ggcggcgcgg ctggaggcga tggttcatgc ggaggacgaa 120

ctctcggagg agcagattca agccaacaac cagacacagg aagatgagct gctggcacta 180

caggccatct atggtgatga tttggttatc ttcgacaata aggacggcct tcgatttttc 240

cagatttctc tgcactatca actcgccggt gacatccgag tttacctgaa tgtctgcccc 300

aacgggagaa cagaaaccgg agcagaaaac gacgacgacg acgacagtga ccgactcttg 360

tatgcttgca gcctgcagca tctgcctcct gttgtgctag cctgcctact accacgttta 420

tatccgagtc accgtgcccc ctacttcgtg gtcgccgcga agtggctgga cgagccggaa 480

gtttcaagct tctgctctgt tcttgatgag atctgggcag agcagcctgc agggcaagag 540

gtggtgtaca aatgggtgga ctggctgagc acctcttcct ggttttgcat tgcttcagat 600

gatcagattg tatttggacc agatgcagac tcggctggtg gtgatgaccg ggcaatcgga 660

agaagctgct cccttgattc tatgatccct ctaattcaac gttacagcaa ggagagatca 720

catgaaattt tcgctcgaag aatccatgag tgcggagtct gtctcagtga aaatacaggc 780

agaaacttca tacagctccc atgcagccac tccttctgcg tcaagtgcat ggagacgcag 840

tgcaggatcc acgtgaagga agggagcgtg gcgaggctga catgcccgga cacgtcgtgc 900

cgccggccgc tgccgccggc gctgctgagg ggcctcctcg gcgacggcga gtacgcgcgg 960

tgggagtcgc tggtgctgcg aggatgctgg acacgatgcc cgacgtggcc tactgcccca 1020

ggtgcagcgc cgcgtgcgtg gcggccggcg acgacgccca gtgctcgagg tgcttcttca 1080

ccttctgcgc cgtctgccgg gagcggcgcc acgtcgggga cacctgcgtc tccccgaacc 1140

agatgctcga catcatgctg gaacggcaga aggagaagag gccgttggcg gcgccgtcgc 1200

cggacagcca ggcggtgtcg tcgaagagga agatggagga gcttctga 1248

<210> 9

<211> 415

<212> PRT

<213> Oryza sativa

<400> 9

Met Met Thr Pro Gly Ser Ser Ser Thr Ser Val Pro Gly Asp Glu Ala

1 5 10 15

Asp Ala Gly Asn Trp Asp Ala Gly Val Glu Thr Ala Ala Arg Leu Glu

20 25 30

Ala Met Val His Ala Glu Asp Glu Leu Ser Glu Glu Gln Ile Gln Ala

35 40 45

Asn Asn Gln Thr Gln Glu Asp Glu Leu Leu Ala Leu Gln Ala Ile Tyr

50 55 60

Gly Asp Asp Leu Val Ile Phe Asp Asn Lys Asp Gly Leu Arg Phe Phe

65 70 75 80

Gln Ile Ser Leu His Tyr Gln Leu Ala Gly Asp Ile Arg Val Tyr Leu

85 90 95

Asn Val Cys Pro Asn Gly Arg Thr Glu Thr Gly Ala Glu Asn Asp Asp

100 105 110

Asp Asp Asp Ser Asp Arg Leu Leu Tyr Ala Cys Ser Leu Gln His Leu

115 120 125

Pro Pro Val Val Leu Ala Cys Leu Leu Pro Arg Leu Tyr Pro Ser His

130 135 140

Arg Ala Pro Tyr Phe Val Val Ala Ala Lys Trp Leu Asp Glu Pro Glu

145 150 155 160

Val Ser Ser Phe Cys Ser Val Leu Asp Glu Ile Trp Ala Glu Gln Pro

165 170 175

Ala Gly Gln Glu Val Val Tyr Lys Trp Val Asp Trp Leu Ser Thr Ser

180 185 190

Ser Trp Phe Cys Ile Ala Ser Asp Asp Gln Ile Val Phe Gly Pro Asp

195 200 205

Ala Asp Ser Ala Gly Gly Asp Asp Arg Ala Ile Gly Arg Ser Cys Ser

210 215 220

Leu Asp Ser Met Ile Pro Leu Ile Gln Arg Tyr Ser Lys Glu Arg Ser

225 230 235 240

His Glu Ile Phe Ala Arg Arg Ile His Glu Cys Gly Val Cys Leu Ser

245 250 255

Glu Asn Thr Gly Arg Asn Phe Ile Gln Leu Pro Cys Ser His Ser Phe

260 265 270

Cys Val Lys Cys Met Glu Thr Gln Cys Arg Ile His Val Lys Glu Gly

275 280 285

Ser Val Ala Arg Leu Thr Cys Pro Asp Thr Ser Cys Arg Arg Pro Leu

290 295 300

Pro Pro Ala Leu Leu Arg Gly Leu Leu Gly Asp Gly Glu Tyr Ala Arg

305 310 315 320

Trp Glu Ser Leu Val Leu Arg Gly Cys Trp Thr Arg Cys Pro Thr Trp

325 330 335

Pro Thr Ala Pro Gly Ala Ala Pro Arg Ala Trp Arg Pro Ala Thr Thr

340 345 350

Pro Ser Ala Arg Gly Ala Ser Ser Pro Ser Ala Pro Ser Ala Gly Ser

355 360 365

Gly Ala Thr Ser Gly Thr Pro Ala Ser Pro Arg Thr Arg Cys Ser Thr

370 375 380

Ser Cys Trp Asn Gly Arg Arg Arg Arg Gly Arg Trp Arg Arg Arg Arg

385 390 395 400

Arg Thr Ala Arg Arg Cys Arg Arg Arg Gly Arg Trp Arg Ser Phe

405 410 415

<210> 10

<211> 1827

<212> DNA

<213> Oryza sativa

<400> 10

gatcaatctc tcgcttcctt cccgtgaaga tgtcgtcgcc cgcgggggag gatgagaaga 60

agccggcggg gggagagggc ggcggcgccc acatcaacct caaggtcaag ggacaggtgc 120

gttttcctcc tgccccttca cctgggatgg agcgatcccg tggcggggtt gattgcgtga 180

tcggaatcgc gtcgattcta tctagatgtg aatgtgttcg cttcctttgt agggattatg 240

gggcgtctgc tcttgcgatc gaaatcgcga tcggaattgc gttgtgatgt ggtcttaggg 300

tttgtaggtt ggaatttcaa ttccctattt gggcgttgtt tgatataagt ttgggtagaa 360

gaggaagttt tagccgtagg aattatcgat cgatttgtgc tagggcatgg ccgacctttg 420

agtttctagc agcgaggtac tcctacatga tgggagaatt ggttaagcac ataggggttg 480

tttatcatca gtaaaaattc cagtccaata caacttactc ttgatcattg gtaattttgt 540

ttgaggcgtt aaccttgata tggtactctc ttatgatttt ttgttccgag tatacatgat 600

aatttagttg aattttcatt cagattatca gttcctatga tgttttagtt gatgctggtg 660

ggtatcatac tctcttagtt cgcaatggct gaaaggagtt tttgttcagc gatacagaac 720

aatttgttga agccaaatgg gatgtttcat tgctcagtat ctccttcata cagcattgat 780

cataattagt ttaaacccta gttagttgca ttctcgattt agactctggc attctcgaac 840

atctgagtca taggcacaca actatgcaca tcctttggct agttaattgt aatgatattc 900

agagcaaatc tgatctgtgt atgcctgttg cattttctgt ctggaattgt taatatggtt 960

tttttcaaat actgccttat aggacatttt catgtaccag ggctgagttt ttttgtgtta 1020

aatactcata aaggggagac ttgctagttg catgcatatc ataagttgca gacaattctc 1080

aatttctgag tgtccagata aaatataatg gcaagaattt gagaatatgc atttgtctgt 1140

ggttgtgtgc taataggata ttatacttca attcaaaagt tttagatcac tcataaattt 1200

gcatcaactt tgaagttgaa tcatttatca attgagtcac tacgctgcaa attgaaaaca 1260

atgcatttat ttgtttgcga tgatgagatg ttgccagatt tttttttctt ttgttagtaa 1320

agtgcatttt atgttttggc gccataaaac cttatcattc cttgtgctgt aggatggcaa 1380

cgaggtgttc tttcgtatca agagatcaac ccaactgaag aagctgatga acgcctactg 1440

tgatcgccag tccgtggata tcaaatctat tgccttcctg tttgatggtc gtaggctcaa 1500

tgctgagcag acccctgacc aggtgcgtga tataccatac tgccttctct aaacaatgag 1560

ctgatgcaaa tactgccttc cctaaaaccc agtgctatag ctgatgcttt tttaccactg 1620

caaactgttt gatttcagct cgagatggaa gacggcgacg agattgacgc catgcttcac 1680

cagactgggg gctctctgcc tgcctagagc tcatatgaag ctgttgcaac aatgcaaccg 1740

cattactatt ttctgccttg ttagagtcag tcagtctaga ggacatgaaa ctagtctaaa 1800

gaacttgtga atcgtttggt cctgtgc 1827

<210> 11

<211> 306

<212> DNA

<213> Oryza sativa

<400> 11

atgtcgtcgc ccgcggggga ggatgagaag aagccggcgg ggggagaggg cggcggcgcc 60

cacatcaacc tcaaggtcaa gggacaggat ggcaacgagg tgttctttcg tatcaagaga 120

tcaacccaac tgaagaagct gatgaacgcc tactgtgatc gccagtccgt ggatatcaaa 180

tctattgcct tcctgtttga tggtcgtagg ctcaatgctg agcagacccc tgaccagctc 240

gagatggaag acggcgacga gattgacgcc atgcttcacc agactggggg ctctctgcct 300

gcctag 306

<210> 12

<211> 101

<212> PRT

<213> Oryza sativa

<400> 12

Met Ser Ser Pro Ala Gly Glu Asp Glu Lys Lys Pro Ala Gly Gly Glu

1 5 10 15

Gly Gly Gly Ala His Ile Asn Leu Lys Val Lys Gly Gln Asp Gly Asn

20 25 30

Glu Val Phe Phe Arg Ile Lys Arg Ser Thr Gln Leu Lys Lys Leu Met

35 40 45

Asn Ala Tyr Cys Asp Arg Gln Ser Val Asp Ile Lys Ser Ile Ala Phe

50 55 60

Leu Phe Asp Gly Arg Arg Leu Asn Ala Glu Gln Thr Pro Asp Gln Leu

65 70 75 80

Glu Met Glu Asp Gly Asp Glu Ile Asp Ala Met Leu His Gln Thr Gly

85 90 95

Gly Ser Leu Pro Ala

100

<210> 13

<211> 2186

<212> DNA

<213> Oryza sativa

<400> 13

gtcgaaaccg agttgatctc gctatgctcc gagtcggatt ccagcttggc acacggaagc 60

cgcgaacgaa agcgtatttt gtgattacgt ttagggtggc tctattttga tttctacgga 120

atacgtattc cttgtttgaa cacggcttta gttagtaaca ctcaccaatt gggacgaaca 180

cctcgactcc tcgagtcacc aatggcgatt ccccgtcgcc tcgccgccgc cgcggccgcc 240

gaaaccacca aacgctcggc ggcaggtctc gccgccgccc tcggtggaag cggagggaag 300

cccgcaacag ccgacctggc cgctgccgcg acggccgcgg cggccgccgg tcgcgcctcc 360

gaatgccagt ccctcctcct ccgcatgtcg cgccgccgcg gcgcctgccg tcgcgagatc 420

gtctcctccc tcctcggctc ctcccccacc ccgcagccgc gggtgtttga cctcctaatc 480

cgcacctaca cccagtcccg caagccccgc gaggccttcg aggcattccg cctcatcctc 540

gaccaccgcg tccccatccc cgccgccgcc tccaacgccc tgctcgccgc cctctcccgc 600

gccggatggc cccatctcgc cgcggacgcc taccgccttg tcttctcctc caactccgag 660

gtaaacacgt acacgcttaa cataatggtc cacaactact gcaaagccct ggagttcgac 720

aaggttgacg ctgtcatctc cgagatggag aagagatgtg tctttcctga tgtggttaca 780

cataatgtga tggttgatgc tagatttcgc gctggggacg cggaggcagc aatggcgttg 840

gttgactcaa tggttagtaa agggctaaag cctgggattg tgacgtataa ttcggttctg 900

aaagggttat gtaggagtgg gatgtgggat aaagcatggg aagtgttcaa agaaatggat 960

gattttggtg ttgcgcctga tgttcggagt tttaccattt tgattggggg attttgtaga 1020

gttggggaga ttgaggaggc gttgaagatt tacaaggaga tgcggcaccg tggtattaaa 1080

ccagatttgg tgagctttag ttgcttaatt ggattgtttg caaggagggg gaagatggac 1140

catgcgatgg cgtacttgag ggagatgagg tgctttggat tggtacccga tggtgtgatt 1200

tacacaatgg taataggcgg attttgtagg gctgggttaa tgtcagatgc tctgagagtt 1260

agggatgaga tggttggctg tggatgtttg ccagatgtgg taacttacaa tactttgttg 1320

aatgggctct gtaaagagcg caggttgtta gatgcagaag ggcttttgaa tgagatgagg 1380

gagagagggg ttccaccaga tttatgtacc ttcacaactt tgattcacgg gtattgcata 1440

gagggtaaac tagacaaggc gctgcaactg tttgacacaa tgttgaacca gcgtttgagg 1500

ccagacatag taacatataa tactttgatc gacggaatgt gcagacaagg tgatcttgac 1560

aaagccaatg atctatggga tgatatgcat tctcgtgaaa tcttccccaa tcatgttacg 1620

tacagtatcc taatcgacag tcactgtgag aagggacaag tggaagatgc atttggtttt 1680

ttggatgaaa tgataaataa gggcattttg ccaaacatca tgacatataa ttccatcatt 1740

aagggctatt gccggtctgg aaatgtttca aaggggcaga agttcttgca aaagatgatg 1800

gtcaacaagg tgtcacctga tttaattact tacaacaccc taatccatgg ttatatcaaa 1860

gaagataaga tgcacgatgc ttttaagttg cttaacatga tggagaagga aaaggttcaa 1920

ccagatgttg tcacatataa tatgctcata aatgggtttt ctgtacatgg taatgtgcaa 1980

gaagctggtt ggatttttga gaaaatgtgt gctaaaggaa ttgaaccaga tagatataca 2040

tacatgtcta tgataaatgg tcatgtcaca gctggcaact cgaaggaggc attccagctt 2100

catgatgaga tgcttcaaag agggtttgct cctgacgata aattctgagg gtcctcattt 2160

tcttctttat tggattccgt agagcc 2186

<210> 14

<211> 1947

<212> DNA

<213> Oryza sativa

<400> 14

atggcgattc cccgtcgcct cgccgccgcc gcggccgccg aaaccaccaa acgctcggcg 60

gcaggtctcg ccgccgccct cggtggaagc ggagggaagc ccgcaacagc cgacctggcc 120

gctgccgcga cggccgcggc ggccgccggt cgcgcctccg aatgccagtc cctcctcctc 180

cgcatgtcgc gccgccgcgg cgcctgccgt cgcgagatcg tctcctccct cctcggctcc 240

tcccccaccc cgcagccgcg ggtgtttgac ctcctaatcc gcacctacac ccagtcccgc 300

aagccccgcg aggccttcga ggcattccgc ctcatcctcg accaccgcgt ccccatcccc 360

gccgccgcct ccaacgccct gctcgccgcc ctctcccgcg ccggatggcc ccatctcgcc 420

gcggacgcct accgccttgt cttctcctcc aactccgagg taaacacgta cacgcttaac 480

ataatggtcc acaactactg caaagccctg gagttcgaca aggttgacgc tgtcatctcc 540

gagatggaga agagatgtgt ctttcctgat gtggttacac ataatgtgat ggttgatgct 600

agatttcgcg ctggggacgc ggaggcagca atggcgttgg ttgactcaat ggttagtaaa 660

gggctaaagc ctgggattgt gacgtataat tcggttctga aagggttatg taggagtggg 720

atgtgggata aagcatggga agtgttcaaa gaaatggatg attttggtgt tgcgcctgat 780

gttcggagtt ttaccatttt gattggggga ttttgtagag ttggggagat tgaggaggcg 840

ttgaagattt acaaggagat gcggcaccgt ggtattaaac cagatttggt gagctttagt 900

tgcttaattg gattgtttgc aaggaggggg aagatggacc atgcgatggc gtacttgagg 960

gagatgaggt gctttggatt ggtacccgat ggtgtgattt acacaatggt aataggcgga 1020

ttttgtaggg ctgggttaat gtcagatgct ctgagagtta gggatgagat ggttggctgt 1080

ggatgtttgc cagatgtggt aacttacaat actttgttga atgggctctg taaagagcgc 1140

aggttgttag atgcagaagg gcttttgaat gagatgaggg agagaggggt tccaccagat 1200

ttatgtacct tcacaacttt gattcacggg tattgcatag agggtaaact agacaaggcg 1260

ctgcaactgt ttgacacaat gttgaaccag cgtttgaggc cagacatagt aacatataat 1320

actttgatcg acggaatgtg cagacaaggt gatcttgaca aagccaatga tctatgggat 1380

gatatgcatt ctcgtgaaat cttccccaat catgttacgt acagtatcct aatcgacagt 1440

cactgtgaga agggacaagt ggaagatgca tttggttttt tggatgaaat gataaataag 1500

ggcattttgc caaacatcat gacatataat tccatcatta agggctattg ccggtctgga 1560

aatgtttcaa aggggcagaa gttcttgcaa aagatgatgg tcaacaaggt gtcacctgat 1620

ttaattactt acaacaccct aatccatggt tatatcaaag aagataagat gcacgatgct 1680

tttaagttgc ttaacatgat ggagaaggaa aaggttcaac cagatgttgt cacatataat 1740

atgctcataa atgggttttc tgtacatggt aatgtgcaag aagctggttg gatttttgag 1800

aaaatgtgtg ctaaaggaat tgaaccagat agatatacat acatgtctat gataaatggt 1860

catgtcacag ctggcaactc gaaggaggca ttccagcttc atgatgagat gcttcaaaga 1920

gggtttgctc ctgacgataa attctga 1947

<210> 15

<211> 648

<212> PRT

<213> Oryza sativa

<400> 15

Met Ala Ile Pro Arg Arg Leu Ala Ala Ala Ala Ala Ala Glu Thr Thr

1 5 10 15

Lys Arg Ser Ala Ala Gly Leu Ala Ala Ala Leu Gly Gly Ser Gly Gly

20 25 30

Lys Pro Ala Thr Ala Asp Leu Ala Ala Ala Ala Thr Ala Ala Ala Ala

35 40 45

Ala Gly Arg Ala Ser Glu Cys Gln Ser Leu Leu Leu Arg Met Ser Arg

50 55 60

Arg Arg Gly Ala Cys Arg Arg Glu Ile Val Ser Ser Leu Leu Gly Ser

65 70 75 80

Ser Pro Thr Pro Gln Pro Arg Val Phe Asp Leu Leu Ile Arg Thr Tyr

85 90 95

Thr Gln Ser Arg Lys Pro Arg Glu Ala Phe Glu Ala Phe Arg Leu Ile

100 105 110

Leu Asp His Arg Val Pro Ile Pro Ala Ala Ala Ser Asn Ala Leu Leu

115 120 125

Ala Ala Leu Ser Arg Ala Gly Trp Pro His Leu Ala Ala Asp Ala Tyr

130 135 140

Arg Leu Val Phe Ser Ser Asn Ser Glu Val Asn Thr Tyr Thr Leu Asn

145 150 155 160

Ile Met Val His Asn Tyr Cys Lys Ala Leu Glu Phe Asp Lys Val Asp

165 170 175

Ala Val Ile Ser Glu Met Glu Lys Arg Cys Val Phe Pro Asp Val Val

180 185 190

Thr His Asn Val Met Val Asp Ala Arg Phe Arg Ala Gly Asp Ala Glu

195 200 205

Ala Ala Met Ala Leu Val Asp Ser Met Val Ser Lys Gly Leu Lys Pro

210 215 220

Gly Ile Val Thr Tyr Asn Ser Val Leu Lys Gly Leu Cys Arg Ser Gly

225 230 235 240

Met Trp Asp Lys Ala Trp Glu Val Phe Lys Glu Met Asp Asp Phe Gly

245 250 255

Val Ala Pro Asp Val Arg Ser Phe Thr Ile Leu Ile Gly Gly Phe Cys

260 265 270

Arg Val Gly Glu Ile Glu Glu Ala Leu Lys Ile Tyr Lys Glu Met Arg

275 280 285

His Arg Gly Ile Lys Pro Asp Leu Val Ser Phe Ser Cys Leu Ile Gly

290 295 300

Leu Phe Ala Arg Arg Gly Lys Met Asp His Ala Met Ala Tyr Leu Arg

305 310 315 320

Glu Met Arg Cys Phe Gly Leu Val Pro Asp Gly Val Ile Tyr Thr Met

325 330 335

Val Ile Gly Gly Phe Cys Arg Ala Gly Leu Met Ser Asp Ala Leu Arg

340 345 350

Val Arg Asp Glu Met Val Gly Cys Gly Cys Leu Pro Asp Val Val Thr

355 360 365

Tyr Asn Thr Leu Leu Asn Gly Leu Cys Lys Glu Arg Arg Leu Leu Asp

370 375 380

Ala Glu Gly Leu Leu Asn Glu Met Arg Glu Arg Gly Val Pro Pro Asp

385 390 395 400

Leu Cys Thr Phe Thr Thr Leu Ile His Gly Tyr Cys Ile Glu Gly Lys

405 410 415

Leu Asp Lys Ala Leu Gln Leu Phe Asp Thr Met Leu Asn Gln Arg Leu

420 425 430

Arg Pro Asp Ile Val Thr Tyr Asn Thr Leu Ile Asp Gly Met Cys Arg

435 440 445

Gln Gly Asp Leu Asp Lys Ala Asn Asp Leu Trp Asp Asp Met His Ser

450 455 460

Arg Glu Ile Phe Pro Asn His Val Thr Tyr Ser Ile Leu Ile Asp Ser

465 470 475 480

His Cys Glu Lys Gly Gln Val Glu Asp Ala Phe Gly Phe Leu Asp Glu

485 490 495

Met Ile Asn Lys Gly Ile Leu Pro Asn Ile Met Thr Tyr Asn Ser Ile

500 505 510

Ile Lys Gly Tyr Cys Arg Ser Gly Asn Val Ser Lys Gly Gln Lys Phe

515 520 525

Leu Gln Lys Met Met Val Asn Lys Val Ser Pro Asp Leu Ile Thr Tyr

530 535 540

Asn Thr Leu Ile His Gly Tyr Ile Lys Glu Asp Lys Met His Asp Ala

545 550 555 560

Phe Lys Leu Leu Asn Met Met Glu Lys Glu Lys Val Gln Pro Asp Val

565 570 575

Val Thr Tyr Asn Met Leu Ile Asn Gly Phe Ser Val His Gly Asn Val

580 585 590

Gln Glu Ala Gly Trp Ile Phe Glu Lys Met Cys Ala Lys Gly Ile Glu

595 600 605

Pro Asp Arg Tyr Thr Tyr Met Ser Met Ile Asn Gly His Val Thr Ala

610 615 620

Gly Asn Ser Lys Glu Ala Phe Gln Leu His Asp Glu Met Leu Gln Arg

625 630 635 640

Gly Phe Ala Pro Asp Asp Lys Phe

645

<210> 16

<211> 435

<212> DNA

<213> Oryza sativa

<400> 16

cgatgatggt gatgggctac ttgctggcgc ccaagaaggg cgggaggagg aggaggggga 60

aggatcagcc gtcggcggcg gcgcatcacg gcgataacga cgatggactc cgggagacgc 120

tgctggagca gcagcagcag ccggcgtcgt cgtcgtcgcc gacggccggt ggcggcggcg 180

tgccgaaggg gtacttcgcg gtgtacgtcg gcgaggaggc gcggcggttc gtggtgccca 240

cggggtacct ccgcgagccg gcgttccggg acctcatgga gcgcgccgcc gacgagttcg 300

gcttcgccca ggccggcggc ctccgcgtgc cctgcggcga ggacgacttc gaggatctcc 360

tccgccgcct ccgccgcaag aacggcggcg ccgccgccgc caaggctaag aaagccatca 420

gctagccacg cgtac 435

<210> 17

<211> 423

<212> DNA

<213> Oryza sativa

<400> 17

atgatggtga tgggctactt gctggcgccc aagaagggcg ggaggaggag gagggggaag 60

gatcagccgt cggcggcggc gcatcacggc gataacgacg atggactccg ggagacgctg 120

ctggagcagc agcagcagcc ggcgtcgtcg tcgtcgccga cggccggtgg cggcggcgtg 180

ccgaaggggt acttcgcggt gtacgtcggc gaggaggcgc ggcggttcgt ggtgcccacg 240

gggtacctcc gcgagccggc gttccgggac ctcatggagc gcgccgccga cgagttcggc 300

ttcgcccagg ccggcggcct ccgcgtgccc tgcggcgagg acgacttcga ggatctcctc 360

cgccgcctcc gccgcaagaa cggcggcgcc gccgccgcca aggctaagaa agccatcagc 420

tag 423

<210> 18

<211> 140

<212> PRT

<213> Oryza sativa

<400> 18

Met Met Val Met Gly Tyr Leu Leu Ala Pro Lys Lys Gly Gly Arg Arg

1 5 10 15

Arg Arg Gly Lys Asp Gln Pro Ser Ala Ala Ala His His Gly Asp Asn

20 25 30

Asp Asp Gly Leu Arg Glu Thr Leu Leu Glu Gln Gln Gln Gln Pro Ala

35 40 45

Ser Ser Ser Ser Pro Thr Ala Gly Gly Gly Gly Val Pro Lys Gly Tyr

50 55 60

Phe Ala Val Tyr Val Gly Glu Glu Ala Arg Arg Phe Val Val Pro Thr

65 70 75 80

Gly Tyr Leu Arg Glu Pro Ala Phe Arg Asp Leu Met Glu Arg Ala Ala

85 90 95

Asp Glu Phe Gly Phe Ala Gln Ala Gly Gly Leu Arg Val Pro Cys Gly

100 105 110

Glu Asp Asp Phe Glu Asp Leu Leu Arg Arg Leu Arg Arg Lys Asn Gly

115 120 125

Gly Ala Ala Ala Ala Lys Ala Lys Lys Ala Ile Ser

130 135 140

<210> 19

<211> 2695

<212> DNA

<213> Oryza sativa

<400> 19

caaatctcac gcggcgcttt ctctctctcc cgcgagatgg acgccgcggc ggcgtcctcc 60

tcctcctcct cgtcggcgac gatggcggcg gcggcggcgt cggccgcgga ggcgtcgttg 120

tccggctcgc ctgcttcttc gaggaacgct cgccatcggc agcgtaaggg ggtccggctc 180

cggatgctgc gacggcgggg gcggcagcct gtggaggccg agcgcgcccc gggcgatggg 240

ggcggtggcg ccgtgcagga ggatctcgcg ctgcctctcg gaatgtcctt cgccgccgtc 300

ctcgcgcagg tgcgtcatgg cgggtggatt tccttatagg caaaccctga agaaagtagt 360

agtactagta cttttttctt ccttggcgct gttcgtgatc ctctctgcga tgttcgtgcc 420

atcaaatgta ctagcatatt gcgtgtagat tctgttgcta gcgtctcacg aacacggtaa 480

tctgagaggt tgcgtgctct ggatggtagc ttagaatggg aaatggaacg ttagacgcgg 540

cagcttgggg ccgaggaaat tctttgtgct atacttccct caatggaaat gtcattgagc 600

caaaataaaa aaaagtcatt ttagttcaca gctatatgct cagatttttc acaaataagg 660

ggaaaaacta ggatgcaaaa tgatgagaac aaggaatgat tatgaataag acagcagaga 720

aattgggctt gacaatgttg tggctttatg ggttatgcaa gccttgtact tttgtcaaat 780

aagtagtgtg gcatttaccg tcttattgca aaattcaact tcattgctac tattattctt 840

catttccata cttatagtgg acatgaaatt aaatagtcat ttcgataata ctaccccgtc 900

atcctgtcat tggacattaa attagtcaca gttcagcatc tcttcatccg aaagcttgca 960

ctatagcagc attttgaaca cagatgcata tgcatgtgca aagtctatct aatattcacc 1020

tgcgtacatc tggacatttg cattagtaat tataaataaa catgtttgta caccggttta 1080

gccctatgcc cttattgttc aggtaacaag gagctgcttt cgttgaccgc aggttataaa 1140

tacaaagaat atttcaggac aaagattaca tcctgatttc ctctccaagg tacgtgcttt 1200

ctaatttgat ttatgaactc tgttatccat aaacccacaa attgatggtc tgaaattggg 1260

tttgagtgta gatctgtaca tcagcagtca aggaatctct gacaaatgta agcccggcta 1320

ccactattat tcagttctca ttgtctctta gtattcattg taatatcctt taattatcgt 1380

ttctgtagat atatggtgac agttccaaca gtttcatcaa aaatttcgaa aaatctttca 1440

gcagcacttt taggacgctt catcttgtta atgaaatacc tgtgaatgag agaagtcata 1500

ttcctgaatg ttcttttaaa catgatgatt ctgtggctgt ggacagcttg agctcatctg 1560

atttgcaaaa tcagacaaac agaatagagc atgaccttgt gaacacagtg gaaagtcaac 1620

tagttctctt tgccagtgac aatcagcagt taacgcatct tcggcatagc agatcctctc 1680

ctgaagctga taatcgcatt cttaatgcca ttgatcgctc gaacgaactc aaggaatttg 1740

aaatagggct taccatgaga aagttgcaac tcaaacagtc acagttagct cttagttccc 1800

attcacacat gttagagaag attaagctat ctttcggatt tcagaaagct tccttcaaag 1860

gagagaaatt caagactcgc atgcaggaga caagagacgc agaaatcctt aggacgctaa 1920

tagattttct tgttagtgct gtgatagtta tgtcagcgtg ctttggatat ggaacttata 1980

tttattcgta tcaaaggata acggatgtta cgtcagcttg ttctgccaca tcaaaggtat 2040

gtttatgtag tacatctgtt atcactgagt agttttcact tatcgtgtct ctttagtaat 2100

cggaagctgt ttttttccca ccctgtctct ttcagggatc taagtcatgg tggatgccaa 2160

attcagtgtc gaacttcagt tctgggtttc tttttctcag gtgtcatgta attgccgtaa 2220

cacgtatgtg ctttggcata ctaatgatcc tggcaattgc ttggttagca tttcagcgtt 2280

cctcaactac tgggtcaaat atgcctataa cattcaatct cattttgctg ggaattatct 2340

gcggctttgc tggaaggttt tgtaccaata cactaggtgg cgatggaaac acttggctca 2400

tgtactggga ggttctttgt tctatccact tacttggaaa tctttttcca tctcttttat 2460

accacgttct acatggacct atttcagtat ctcacagaga gcaagttgtc tggttaccat 2520

actgggttcg ccggtgcttg ttttatgctg cggtggggct tattcttcca gccttgactg 2580

gcttgctccc gtttgcctct ctttctgact ggaaggatca ttttgtggaa gagataaaat 2640

ccattgttat cggtgacaag attgaagcat gaaatttctt ggatgtaagg tggcg 2695

<210> 20

<211> 1551

<212> DNA

<213> Oryza sativa

<400> 20

atggacgccg cggcggcgtc ctcctcctcc tcctcgtcgg cgacgatggc ggcggcggcg 60

gcgtcggccg cggaggcgtc gttgtccggc tcgcctgctt cttcgaggaa cgctcgccat 120

cggcagcgta agggggtccg gctccggatg ctgcgacggc gggggcggca gcctgtggag 180

gccgagcgcg ccccgggcga tgggggcggt ggcgccgtgc aggaggatct cgcgctgcct 240

ctcggaatgt ccttcgccgc cgtcctcgcg caggttataa atacaaagaa tatttcagga 300

caaagattac atcctgattt cctctccaag atctgtacat cagcagtcaa ggaatctctg 360

acaaatatat atggtgacag ttccaacagt ttcatcaaaa atttcgaaaa atctttcagc 420

agcactttta ggacgcttca tcttgttaat gaaatacctg tgaatgagag aagtcatatt 480

cctgaatgtt cttttaaaca tgatgattct gtggctgtgg acagcttgag ctcatctgat 540

ttgcaaaatc agacaaacag aatagagcat gaccttgtga acacagtgga aagtcaacta 600

gttctctttg ccagtgacaa tcagcagtta acgcatcttc ggcatagcag atcctctcct 660

gaagctgata atcgcattct taatgccatt gatcgctcga acgaactcaa ggaatttgaa 720

atagggctta ccatgagaaa gttgcaactc aaacagtcac agttagctct tagttcccat 780

tcacacatgt tagagaagat taagctatct ttcggatttc agaaagcttc cttcaaagga 840

gagaaattca agactcgcat gcaggagaca agagacgcag aaatccttag gacgctaata 900

gattttcttg ttagtgctgt gatagttatg tcagcgtgct ttggatatgg aacttatatt 960

tattcgtatc aaaggataac ggatgttacg tcagcttgtt ctgccacatc aaagggatct 1020

aagtcatggt ggatgccaaa ttcagtgtcg aacttcagtt ctgggtttct ttttctcagg 1080

tgtcatgtaa ttgccgtaac acgtatgtgc tttggcatac taatgatcct ggcaattgct 1140

tggttagcat ttcagcgttc ctcaactact gggtcaaata tgcctataac attcaatctc 1200

attttgctgg gaattatctg cggctttgct ggaaggtttt gtaccaatac actaggtggc 1260

gatggaaaca cttggctcat gtactgggag gttctttgtt ctatccactt acttggaaat 1320

ctttttccat ctcttttata ccacgttcta catggaccta tttcagtatc tcacagagag 1380

caagttgtct ggttaccata ctgggttcgc cggtgcttgt tttatgctgc ggtggggctt 1440

attcttccag ccttgactgg cttgctcccg tttgcctctc tttctgactg gaaggatcat 1500

tttgtggaag agataaaatc cattgttatc ggtgacaaga ttgaagcatg a 1551

<210> 21

<211> 516

<212> PRT

<213> Oryza sativa

<400> 21

Met Asp Ala Ala Ala Ala Ser Ser Ser Ser Ser Ser Ser Ala Thr Met

1 5 10 15

Ala Ala Ala Ala Ala Ser Ala Ala Glu Ala Ser Leu Ser Gly Ser Pro

20 25 30

Ala Ser Ser Arg Asn Ala Arg His Arg Gln Arg Lys Gly Val Arg Leu

35 40 45

Arg Met Leu Arg Arg Arg Gly Arg Gln Pro Val Glu Ala Glu Arg Ala

50 55 60

Pro Gly Asp Gly Gly Gly Gly Ala Val Gln Glu Asp Leu Ala Leu Pro

65 70 75 80

Leu Gly Met Ser Phe Ala Ala Val Leu Ala Gln Val Ile Asn Thr Lys

85 90 95

Asn Ile Ser Gly Gln Arg Leu His Pro Asp Phe Leu Ser Lys Ile Cys

100 105 110

Thr Ser Ala Val Lys Glu Ser Leu Thr Asn Ile Tyr Gly Asp Ser Ser

115 120 125

Asn Ser Phe Ile Lys Asn Phe Glu Lys Ser Phe Ser Ser Thr Phe Arg

130 135 140

Thr Leu His Leu Val Asn Glu Ile Pro Val Asn Glu Arg Ser His Ile

145 150 155 160

Pro Glu Cys Ser Phe Lys His Asp Asp Ser Val Ala Val Asp Ser Leu

165 170 175

Ser Ser Ser Asp Leu Gln Asn Gln Thr Asn Arg Ile Glu His Asp Leu

180 185 190

Val Asn Thr Val Glu Ser Gln Leu Val Leu Phe Ala Ser Asp Asn Gln

195 200 205

Gln Leu Thr His Leu Arg His Ser Arg Ser Ser Pro Glu Ala Asp Asn

210 215 220

Arg Ile Leu Asn Ala Ile Asp Arg Ser Asn Glu Leu Lys Glu Phe Glu

225 230 235 240

Ile Gly Leu Thr Met Arg Lys Leu Gln Leu Lys Gln Ser Gln Leu Ala

245 250 255

Leu Ser Ser His Ser His Met Leu Glu Lys Ile Lys Leu Ser Phe Gly

260 265 270

Phe Gln Lys Ala Ser Phe Lys Gly Glu Lys Phe Lys Thr Arg Met Gln

275 280 285

Glu Thr Arg Asp Ala Glu Ile Leu Arg Thr Leu Ile Asp Phe Leu Val

290 295 300

Ser Ala Val Ile Val Met Ser Ala Cys Phe Gly Tyr Gly Thr Tyr Ile

305 310 315 320

Tyr Ser Tyr Gln Arg Ile Thr Asp Val Thr Ser Ala Cys Ser Ala Thr

325 330 335

Ser Lys Gly Ser Lys Ser Trp Trp Met Pro Asn Ser Val Ser Asn Phe

340 345 350

Ser Ser Gly Phe Leu Phe Leu Arg Cys His Val Ile Ala Val Thr Arg

355 360 365

Met Cys Phe Gly Ile Leu Met Ile Leu Ala Ile Ala Trp Leu Ala Phe

370 375 380

Gln Arg Ser Ser Thr Thr Gly Ser Asn Met Pro Ile Thr Phe Asn Leu

385 390 395 400

Ile Leu Leu Gly Ile Ile Cys Gly Phe Ala Gly Arg Phe Cys Thr Asn

405 410 415

Thr Leu Gly Gly Asp Gly Asn Thr Trp Leu Met Tyr Trp Glu Val Leu

420 425 430

Cys Ser Ile His Leu Leu Gly Asn Leu Phe Pro Ser Leu Leu Tyr His

435 440 445

Val Leu His Gly Pro Ile Ser Val Ser His Arg Glu Gln Val Val Trp

450 455 460

Leu Pro Tyr Trp Val Arg Arg Cys Leu Phe Tyr Ala Ala Val Gly Leu

465 470 475 480

Ile Leu Pro Ala Leu Thr Gly Leu Leu Pro Phe Ala Ser Leu Ser Asp

485 490 495

Trp Lys Asp His Phe Val Glu Glu Ile Lys Ser Ile Val Ile Gly Asp

500 505 510

Lys Ile Glu Ala

515

<210> 22

<211> 1308

<212> DNA

<213> Oryza sativa

<400> 22

caaccatgga cgacagcagc agtagcagca gcgaggttga gctgatgctc gagagggcgt 60

tcccggatgg ggtcgagata gacgtcgcca cgcactgcga cggcctcatc gccgtcacga 120

cggacgccgg cgagacattc gtctgcaacc cagccaccaa ggagctcgtc acgctgccgc 180

tcggcatcag ctgccacaac ggctgcgtcg tctgggatcg gttcgcggcc atcggctatg 240

atccgtggcg ccgcgcaacc ggtacgtcgt ctgtaggtac ttccaccggc gctacccaaa 300

cagggccggc gtcgccgaaa tcgggcatga gatcttcgtt ctcggcggcg gcggcgccgg 360

ctcatgggag gccaccgagg atccaccacc aaccagcgcc gtcgtgccca gcactccgcc 420

ggcttgcatc ggagggtgct tctactggtg caccaacgag gacgtcggca acccgagcat 480

gttgctccgg tttagccttc gcagccacaa gttcgacatg gttccatgcc atccgggctg 540

ctcatccgac gtcttcgcat tcaacaccgt gtcggagctg gacggtaagc tgttctacac 600

ccacacagcc acggagacga cgaagacgac ctcccgtctc tggatgcatg ctggacggcg 660

gcggcgacat ggcacggccg gagtggtcca tgcgctgctg catcgatgtc ggcgactacg 720

tgagctgcgt ctccccgctg gtggccggcg gggagcacat cttgctgtcg gtggatgaga 780

acttgtatct gtatggtgag aggagtaggg ttctggagaa ggtggtcgac acggcagagg 840

tggaatacgc gcggtcggat gggagcaaat ataagctagg ctatgatttg tatagtcagc 900

attactatgt tccatatgtg gagagtctag tctcaattag atttcgtaat tattaagaca 960

atcaagtcaa tgtgatggat ttatgaatca ttaggggtct gatccacagg ctcaaaaacc 1020

gccgccgctg ctccatcccc ctccggccct cttcgtccgc gctcaccccc gcttgagcag 1080

ctcgccggaa aggcgggcag cagcaagaac gactgcggcg gagctccttc tctcctcttc 1140

caccccctga tagcactatg cgcctatgcg ggttgtggtc accgaatccc tgtcatgtgg 1200

caatggggtt ctcagatata gaccaatcta tcttctatct atcttctatt atatactaaa 1260

agtccattaa tcttcctaca aacactctca aaccgtcacg tgggattc 1308

<210> 23

<211> 759

<212> DNA

<213> Oryza sativa

<400> 23

atggacgaca gcagcagtag cagcagcgag gttgagctga tgctcgagag ggcgttcccg 60

gatggggtcg agatagacgt cgccacgcac tgcgacggcc tcatcgccgt cacgacggac 120

gccggcgaga cattcgtctg caacccagcc accaaggagc tcgtcacgct gccgctcggc 180

atcagctgcc acaacggctg cgtcgtctgg gatcggttcg cggccatcgg ctatgatccg 240

tggcgccgcg caaccggtac gtcgtctgcc ggcgtcgccg aaatcgggca tgagatcttc 300

gttctcggcg gcggcggcgc cggctcatgg gaggccaccg aggatccacc accaaccagc 360

gccgtcgtgc ccagcactcc gccggcttgc atcggagggt gcttctactg gtgcaccaac 420

gaggacgtcg gcaacccgag catgttgctc cggtttagcc ttcgcagcca caagttcgac 480

atggttccat gccatccggg ctgctcatcc gacgtcttcg cattcaacac cgtgtcggag 540

ctggacggct caaaaaccgc cgccgctgct ccatccccct ccggccctct tcgtccgcgc 600

tcacccccgc ttgagcagct cgccggaaag gcgggcagca gcaagaacga ctgcggcgga 660

gctccttctc tcctcttcca ccccctgata gcactatgcg cctatgcggg ttgtggtcac 720

cgaatccctg tcatgtggca atggggttct cagatatag 759

<210> 24

<211> 252

<212> PRT

<213> Oryza sativa

<400> 24

Met Asp Asp Ser Ser Ser Ser Ser Ser Glu Val Glu Leu Met Leu Glu

1 5 10 15

Arg Ala Phe Pro Asp Gly Val Glu Ile Asp Val Ala Thr His Cys Asp

20 25 30

Gly Leu Ile Ala Val Thr Thr Asp Ala Gly Glu Thr Phe Val Cys Asn

35 40 45

Pro Ala Thr Lys Glu Leu Val Thr Leu Pro Leu Gly Ile Ser Cys His

50 55 60

Asn Gly Cys Val Val Trp Asp Arg Phe Ala Ala Ile Gly Tyr Asp Pro

65 70 75 80

Trp Arg Arg Ala Thr Gly Thr Ser Ser Ala Gly Val Ala Glu Ile Gly

85 90 95

His Glu Ile Phe Val Leu Gly Gly Gly Gly Ala Gly Ser Trp Glu Ala

100 105 110

Thr Glu Asp Pro Pro Pro Thr Ser Ala Val Val Pro Ser Thr Pro Pro

115 120 125

Ala Cys Ile Gly Gly Cys Phe Tyr Trp Cys Thr Asn Glu Asp Val Gly

130 135 140

Asn Pro Ser Met Leu Leu Arg Phe Ser Leu Arg Ser His Lys Phe Asp

145 150 155 160

Met Val Pro Cys His Pro Gly Cys Ser Ser Asp Val Phe Ala Phe Asn

165 170 175

Thr Val Ser Glu Leu Asp Gly Ser Lys Thr Ala Ala Ala Ala Pro Ser

180 185 190

Pro Ser Gly Pro Leu Arg Pro Arg Ser Pro Pro Leu Glu Gln Leu Ala

195 200 205

Gly Lys Ala Gly Ser Ser Lys Asn Asp Cys Gly Gly Ala Pro Ser Leu

210 215 220

Leu Phe His Pro Leu Ile Ala Leu Cys Ala Tyr Ala Gly Cys Gly His

225 230 235 240

Arg Ile Pro Val Met Trp Gln Trp Gly Ser Gln Ile

245 250

<210> 25

<211> 33

<212> DNA

<213> Artificial Sequence

<220>

<223> Forward primer for cloning cDNA of OsDN-DTP8 gene

<400> 25

ctgctgaggg acgatggagc tctggtggat ttc 33

<210> 26

<211> 34

<212> DNA

<213> Artificial Sequence

<220>

<223> Reverse primer for cloning cDNA of OsDN-DTP8 gene

<400> 26

ccgctgaggg tcttctaccc atcccctcaa gtcc 34

<210> 27

<211> 27

<212> DNA

<213> Artificial Sequence

<220>

<223> Forward primer for cloning cDNA of OsKIK1 gene

<400> 27

cgcacacgtg aatacatata ttttctc 27

<210> 28

<211> 24

<212> DNA

<213> Artificial Sequence

<220>

<223> Reverse primer for cloning cDNA of OsKIK1 gene

<400> 28

gcgtgtttct tcgtgtctat gtac 24

<210> 29

<211> 23

<212> DNA

<213> Artificial Sequence

<220>

<223> Forward primer for cloning cDNA of OsRWDD1 gene

<400> 29

ctcgtcgcgg ccgtggaaat tac 23

<210> 30

<211> 24

<212> DNA

<213> Artificial Sequence

<220>

<223> Reverse primer for cloning cDNA of OsRWDD1 gene

<400> 30

gtcttggaca cggccatctt gcac 24

<210> 31

<211> 24

<212> DNA

<213> Artificial Sequence

<220>

<223> Forward primer for cloning gDNA of OsUFD1 gene

<400> 31

gatcaatctc tcgcttcctt cccg 24

<210> 32

<211> 24

<212> DNA

<213> Artificial Sequence

<220>

<223> Reverse primer for cloning gDNA of OsUFD1 gene

<400> 32

gcacaggacc aaacgattca caag 24

<210> 33

<211> 26

<212> DNA

<213> Artificial Sequence

<220>

<223> Forward primer for cloning gDNA of OsPPR2 gene

<400> 33

gtcgaaaccg agttgatctc gctatg 26

<210> 34

<211> 25

<212> DNA

<213> Artificial Sequence

<220>

<223> Reverse primer for cloning gDNA of OsPPR2 gene

<400> 34

ggctctacgg aatccaataa agaag 25

<210> 35

<211> 23

<212> DNA

<213> Artificial Sequence

<220>

<223> Forward primer for cloning cDNA of OsSAUR28-1 gene

<400> 35

cgatgatggt gatgggctac ttg 23

<210> 36

<211> 21

<212> DNA

<213> Artificial Sequence

<220>

<223> Reverse primer for cloning cDNA of OsSAUR28-1 gene

<400> 36

gtacgcgtgg ctagctgatg g 21

<210> 37

<211> 25

<212> DNA

<213> Artificial Sequence

<220>

<223> Forward primer for cloning gDNA of OsHYS1 gene

<400> 37

caaatctcac gcggcgcttt ctctc 25

<210> 38

<211> 28

<212> DNA

<213> Artificial Sequence

<220>

<223> Reverse primer for cloning gDNA of OsHYS1 gene

<400> 38

cgccacctta catccaagaa atttcatg 28

<210> 39

<211> 25

<212> DNA

<213> Artificial Sequence

<220>

<223> Forward primer for cloning gDNA of OsFBID1 gene

<400> 39

caaccatgga cgacagcagc agtag 25

<210> 40

<211> 26

<212> DNA

<213> Artificial Sequence

<220>

<223> Reverse primer for cloning gDNA of OsFBID1 gene

<400> 40

gaatcccacg tgacggtttg agagtg 26

<210> 41

<211> 19

<212> DNA

<213> Artificial Sequence

<220>

<223> Forward primer for real-time PCR analysis of OsDN-DTP8 gene

<400> 41

ccctctcctt cttgcgaac 19

<210> 42

<211> 19

<212> DNA

<213> Artificial Sequence

<220>

<223> Reverse primer for real-time PCR analysis of OsDN-DTP8 gene

<400> 42

tcctcgccat gaaccattg 19

<210> 43

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223> Forward primer for real-time PCR analysis of OsRWDD1 gene

<400> 43

acttcataca gctcccatgc 20

<210> 44

<211> 18

<212> DNA

<213> Artificial Sequence

<220>

<223> Reverse primer for real-time PCR analysis of OsRWDD1 gene

<400> 44

ttcacgtgga tcctgcac 18

<210> 45

<211> 23

<212> DNA

<213> Artificial Sequence

<220>

<223> Forward primer for real-time PCR analysis of OsUFD1 gene

<400> 45

tcaagagatc aacccaactg aag 23

<210> 46

<211> 22

<212> DNA

<213> Artificial Sequence

<220>

<223> Reverse primer for real-time PCR analysis of OsUFD1 gene

<400> 46

ccatcaaaca ggaaggcaat ag 22

<210> 47

<211> 21

<212> DNA

<213> Artificial Sequence

<220>

<223> Forward primer for real-time PCR analysis of OsSAUR28-1 gene

<400> 47

atgatggtga tgggctactt g 21

<210> 48

<211> 19

<212> DNA

<213> Artificial Sequence

<220>

<223> Reverse primer for real-time PCR analysis of OsSAUR28-1 gene

<400> 48

cggagtccat cgtcgttat 19

<210> 49

<211> 21

<212> DNA

<213> Artificial Sequence

<220>

<223> Forward primer for real-time PCR analysis of OsHYS1 gene

<400> 49

agagcaagtt gtctggttac c 21

<210> 50

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223> Reverse primer for real-time PCR analysis of OsHYS1 gene

<400> 50

tcagaaagag aggcaaacgg 20

<210> 51

<211> 264

<212> DNA

<213> Oryza sativa

<400> 51

atggagctct ggtggatttc ggcttcgggg agatggctgc cgagctccct ctccttcttg 60

cgaacccaac ggcggcagcg gcgaccaaat gcggcggcta aggcggctgc tagctgcggc 120

tggagatcgg aacatgacgg caacccaatg gttcatggcg aggacggctt ccggcgaagt 180

ttcggcgcaa ggggaacggc ggccggggtg gacctcgacc ttgcggagcc gatggaggtg 240

gcggcgcagg aaggaggcag atga 264

<210> 52

<211> 87

<212> PRT

<213> Oryza sativa

<400> 52

Met Glu Leu Trp Trp Ile Ser Ala Ser Gly Arg Trp Leu Pro Ser Ser

1 5 10 15

Leu Ser Phe Leu Arg Thr Gln Arg Arg Gln Arg Arg Pro Asn Ala Ala

20 25 30

Ala Lys Ala Ala Ala Ser Cys Gly Trp Arg Ser Glu His Asp Gly Asn

35 40 45

Pro Met Val His Gly Glu Asp Gly Phe Arg Arg Ser Phe Gly Ala Arg

50 55 60

Gly Thr Ala Ala Gly Val Asp Leu Asp Leu Ala Glu Pro Met Glu Val

65 70 75 80

Ala Ala Gln Glu Gly Gly Arg

85

<210> 53

<211> 2562

<212> DNA

<213> Oryza sativa

<400> 53

atggcggctt ctcctcctcc tcctcctcgt cccctgctgc tgcttctccc gttgctgctc 60

gtttggggcg tggtggttgc ggcggcggcg gcgacggata cgttgaggca gggggagtcg 120

ctgacggggg cggcgacgct ggtgtcgtcg ccgtcggggg tgttcgaggt ggggttcttc 180

gcgccggacc cgaagctgcc gtcgcggctc tacctcggca tctggtaccg cagcatctcg 240

ccgagaaccg tcgtctgggt cgccaaccgc gccgcgccgg ccaccgcgcc gtcgccgtcg 300

ctcacgctcg cggccaacgg cgagctccgc gtgctcgacg gctccgccgc ggacgcggac 360

gcgccgctct tgtggaggtc gaacgcgtcc acgcagtccg cgccacgcgg cgggtacaag 420

gccgtcatcc aggacaccgg cagcctcgag gtgcgcagcg acgacggcac gctgtgggac 480

agcttctggc acccgtcgga caccatgctg tccgggatgc gcatcaccgt gcgcacgccc 540

gggaggggcc cctccgagcc gatgcgcttc acgtcgtgga ccagcgagac ggacccctcg 600

ccggggcggt acgcgctcgg cctcgacccg gccaactccg gccaggccta catctggaga 660

gatggcaatg tcaccatttg gaggtcagga caatggactg ggcagaattt tgtgggcatt 720

ccttggagac cactgtactt gtatgggttc aagccagcaa atgatgcaaa cttaggtgca 780

tactacactt acactgcatc aaacacatct ctgcaaaggt ttgttgttat gccaaatggt 840

acagacatct gttacatggt taagaagtca gcacaagagt gggagactgt ctggatgcaa 900

ccatcaaatg agtgtgagta ctatgctacg tgtggcgcaa atgctaaatg cactgccatg 960

caagatggca aggcaaaatg tacctgccta aaaggatttc agccaaagtt gcttgatcag 1020

tggaatatgg gaaattggag tcaaggttgt gtcaggagcc cacctttggg ttgtcaggtg 1080

aaccaaactg gggatggatt tctttctatc ccaaacatca agtggccgga tttctcatac 1140

tggccatcta cggtgcaaga tgagaatgga tgcatgaatg cttgcctcag caactgctca 1200

tgtggtgcct atgtctacat gactacaata ggatgtctac tgtggggtag cgacctgatc 1260

gacatgtacc agtttcagag tgggggatat accctaaacc tcaaacttcc tgcttctgag 1320

ttacgctcac atcatgcagt ttggaaaata gccacaatag tgtctgcagt ggtgctattt 1380

gttttgctag cttgcctctt cctgtggtgg aagcgtggga ggaatatcaa agatgtgatg 1440

cacaaaagtt ggaggtcaat gcatacgtct acacgatctc agcagaatag tggtatgctg 1500

gacatctcgc agtcgattcc ttttgaagac gacacagagg atggaaaaag tcatgaactc 1560

aaagtatact cctttgaccg gataaaagct gccacttgta atttcagtga ctccaacaag 1620

cttggagcag gaggatttgg tcctgtatat atgggaaaat tacctggggg agaagaagta 1680

gccgttaaga ggctttgtag gaaatcaggt caaggccttg aggagttcaa gaatgaggtc 1740

atacttatcg caaaactgca gcatcgcaat cttgtgagac tattaggatg ctgcatacag 1800

ggagaagaga agatcttggt gtacgagtac atgcctaaca aaagtctaga tgcattcctc 1860

ttcaatcctg agaagcaagg gctcctagac tggaggaaac ggtttgatat aattgaaggg 1920

attgctcgag ggctgttata tctccaccgg gactcaaggt tgcgagttgt tcatcgtgat 1980

ctcaaggcca gcaacattct cctggacaaa gacatgaacc ccaagatttc agattttgga 2040

atggccagga tgtttggagg ggatcaaaac caattcaaca cgaatcgtgt ggttggaaca 2100

tttggctata tgtcgccgga atatgctatg gaaggcattt tctcagtaaa gtccgatatt 2160

tatagctttg gagttctaat gttggagatc atcacaggaa agagggcttt gagctttcat 2220

ggtcaacaag actccctaaa cattgcagga tttgcatggc gacaatggaa tgaagacaag 2280

ggcgaggaac tgattgatcc attgataaga gcatcgtgct cacttcgaca agtcttgaga 2340

tgcatccaca ttgcattgct atgtgttcaa gatcatgcgc aagaacgtcc tgatatccct 2400

gcggtcatac tgatgttgag cagcgacagc tccagccttc ccatgccaag accgcctacc 2460

ctgatgctcc atggtcgttc ggctgagacg agcaaatcaa gtgagaagga tcagagccac 2520

tccatcggta ctgtgtcaat gacacaattg catggaagat ag 2562

<210> 54

<211> 853

<212> PRT

<213> Oryza sativa

<400> 54

Met Ala Ala Ser Pro Pro Pro Pro Pro Arg Pro Leu Leu Leu Leu Leu

1 5 10 15

Pro Leu Leu Leu Val Trp Gly Val Val Val Ala Ala Ala Ala Ala Thr

20 25 30

Asp Thr Leu Arg Gln Gly Glu Ser Leu Thr Gly Ala Ala Thr Leu Val

35 40 45

Ser Ser Pro Ser Gly Val Phe Glu Val Gly Phe Phe Ala Pro Asp Pro

50 55 60

Lys Leu Pro Ser Arg Leu Tyr Leu Gly Ile Trp Tyr Arg Ser Ile Ser

65 70 75 80

Pro Arg Thr Val Val Trp Val Ala Asn Arg Ala Ala Pro Ala Thr Ala

85 90 95

Pro Ser Pro Ser Leu Thr Leu Ala Ala Asn Gly Glu Leu Arg Val Leu

100 105 110

Asp Gly Ser Ala Ala Asp Ala Asp Ala Pro Leu Leu Trp Arg Ser Asn

115 120 125

Ala Ser Thr Gln Ser Ala Pro Arg Gly Gly Tyr Lys Ala Val Ile Gln

130 135 140

Asp Thr Gly Ser Leu Glu Val Arg Ser Asp Asp Gly Thr Leu Trp Asp

145 150 155 160

Ser Phe Trp His Pro Ser Asp Thr Met Leu Ser Gly Met Arg Ile Thr

165 170 175

Val Arg Thr Pro Gly Arg Gly Pro Ser Glu Pro Met Arg Phe Thr Ser

180 185 190

Trp Thr Ser Glu Thr Asp Pro Ser Pro Gly Arg Tyr Ala Leu Gly Leu

195 200 205

Asp Pro Ala Asn Ser Gly Gln Ala Tyr Ile Trp Arg Asp Gly Asn Val

210 215 220

Thr Ile Trp Arg Ser Gly Gln Trp Thr Gly Gln Asn Phe Val Gly Ile

225 230 235 240

Pro Trp Arg Pro Leu Tyr Leu Tyr Gly Phe Lys Pro Ala Asn Asp Ala

245 250 255

Asn Leu Gly Ala Tyr Tyr Thr Tyr Thr Ala Ser Asn Thr Ser Leu Gln

260 265 270

Arg Phe Val Val Met Pro Asn Gly Thr Asp Ile Cys Tyr Met Val Lys

275 280 285

Lys Ser Ala Gln Glu Trp Glu Thr Val Trp Met Gln Pro Ser Asn Glu

290 295 300

Cys Glu Tyr Tyr Ala Thr Cys Gly Ala Asn Ala Lys Cys Thr Ala Met

305 310 315 320

Gln Asp Gly Lys Ala Lys Cys Thr Cys Leu Lys Gly Phe Gln Pro Lys

325 330 335

Leu Leu Asp Gln Trp Asn Met Gly Asn Trp Ser Gln Gly Cys Val Arg

340 345 350

Ser Pro Pro Leu Gly Cys Gln Val Asn Gln Thr Gly Asp Gly Phe Leu

355 360 365

Ser Ile Pro Asn Ile Lys Trp Pro Asp Phe Ser Tyr Trp Pro Ser Thr

370 375 380

Val Gln Asp Glu Asn Gly Cys Met Asn Ala Cys Leu Ser Asn Cys Ser

385 390 395 400

Cys Gly Ala Tyr Val Tyr Met Thr Thr Ile Gly Cys Leu Leu Trp Gly

405 410 415

Ser Asp Leu Ile Asp Met Tyr Gln Phe Gln Ser Gly Gly Tyr Thr Leu

420 425 430

Asn Leu Lys Leu Pro Ala Ser Glu Leu Arg Ser His His Ala Val Trp

435 440 445

Lys Ile Ala Thr Ile Val Ser Ala Val Val Leu Phe Val Leu Leu Ala

450 455 460

Cys Leu Phe Leu Trp Trp Lys Arg Gly Arg Asn Ile Lys Asp Val Met

465 470 475 480

His Lys Ser Trp Arg Ser Met His Thr Ser Thr Arg Ser Gln Gln Asn

485 490 495

Ser Gly Met Leu Asp Ile Ser Gln Ser Ile Pro Phe Glu Asp Asp Thr

500 505 510

Glu Asp Gly Lys Ser His Glu Leu Lys Val Tyr Ser Phe Asp Arg Ile

515 520 525

Lys Ala Ala Thr Cys Asn Phe Ser Asp Ser Asn Lys Leu Gly Ala Gly

530 535 540

Gly Phe Gly Pro Val Tyr Met Gly Lys Leu Pro Gly Gly Glu Glu Val

545 550 555 560

Ala Val Lys Arg Leu Cys Arg Lys Ser Gly Gln Gly Leu Glu Glu Phe

565 570 575

Lys Asn Glu Val Ile Leu Ile Ala Lys Leu Gln His Arg Asn Leu Val

580 585 590

Arg Leu Leu Gly Cys Cys Ile Gln Gly Glu Glu Lys Ile Leu Val Tyr

595 600 605

Glu Tyr Met Pro Asn Lys Ser Leu Asp Ala Phe Leu Phe Asn Pro Glu

610 615 620

Lys Gln Gly Leu Leu Asp Trp Arg Lys Arg Phe Asp Ile Ile Glu Gly

625 630 635 640

Ile Ala Arg Gly Leu Leu Tyr Leu His Arg Asp Ser Arg Leu Arg Val

645 650 655

Val His Arg Asp Leu Lys Ala Ser Asn Ile Leu Leu Asp Lys Asp Met

660 665 670

Asn Pro Lys Ile Ser Asp Phe Gly Met Ala Arg Met Phe Gly Gly Asp

675 680 685

Gln Asn Gln Phe Asn Thr Asn Arg Val Val Gly Thr Phe Gly Tyr Met

690 695 700

Ser Pro Glu Tyr Ala Met Glu Gly Ile Phe Ser Val Lys Ser Asp Ile

705 710 715 720

Tyr Ser Phe Gly Val Leu Met Leu Glu Ile Ile Thr Gly Lys Arg Ala

725 730 735

Leu Ser Phe His Gly Gln Gln Asp Ser Leu Asn Ile Ala Gly Phe Ala

740 745 750

Trp Arg Gln Trp Asn Glu Asp Lys Gly Glu Glu Leu Ile Asp Pro Leu

755 760 765

Ile Arg Ala Ser Cys Ser Leu Arg Gln Val Leu Arg Cys Ile His Ile

770 775 780

Ala Leu Leu Cys Val Gln Asp His Ala Gln Glu Arg Pro Asp Ile Pro

785 790 795 800

Ala Val Ile Leu Met Leu Ser Ser Asp Ser Ser Ser Leu Pro Met Pro

805 810 815

Arg Pro Pro Thr Leu Met Leu His Gly Arg Ser Ala Glu Thr Ser Lys

820 825 830

Ser Ser Glu Lys Asp Gln Ser His Ser Ile Gly Thr Val Ser Met Thr

835 840 845

Gln Leu His Gly Arg

850

<210> 55

<211> 2649

<212> DNA

<213> Zea mays

<400> 55

atggcccacg ccgccgcggc agcgctcgcc cttctcgtct tcttctgcgc gcaagcgcgg 60

gacgcggccg tccatgtcgt cgacgcggcg gcgacgctgt cgcaggggca gtcgctgggc 120

gccaccgaca agctggtgtc ggccggcggc accttcgagc tggccttctt cacgccgacg 180

gggggcgacc cgtcgcggcg gtacctgggc gtcatgtacg cgcagtccac cgagcagacc 240

gtgccgtggg tggccaaccg cgacgtgccc gtgagcgcgg gctccgcgta ctcggcgacc 300

gtcacggccg ccggggagct gcaggtactg gagggcgacc gcgtggtctg gcgcaccgac 360

aactccgcga cgacgacgtc gccgggaaca gcgggaggcg aacaagcagc gaacgtgacg 420

ctcaccgtgc tcgacaccgg gaacctccag ctggccgcgg gcgacggggg ccccgtcatc 480

tggcagagct tcgaccaccc cgcggacacc ttcctccccg ggatgagcat caccctggac 540

cggcgcggcg gcggggccgt ccgccggacg ctgttcacgt cgtggcggag ccccgcggac 600

cccggcaccg gggacttcac gctggggcag gacccgctcg gctccgcgca gctctacatc 660

tggcagacga ccggcggcca gaacaccacc tactggcggt ccgggcagtg ggccaacacc 720

aacttcgtcg gcgtgccctg gcgctcgctc tacgtgtacg gcttcaagct caacggcgac 780

cccaacaacg gctccggggt catgtcctac gtcttcaaca cgtacaacag ctccgagtac 840

cggttcatgc tccactccaa cggcaccgag acgtgctaca tgctcctcgc caccggcgac 900

tgggagaccg tctggtcgca gccgaccatc ccgtgccagg cctacaacat gtgcggcgcc 960

aacgcccagt gcgccgccgc cgccgacggc ggccaggcag tctgcacctg cctgacaggt 1020

ttcgagccga ggaacgtatc ggagtacagc aacgggaact ggacgcaggg gtgcgtcagg 1080

agctccccgc tgccctgcgg cggcgagccg aacgtgagcg gcgctggcgc cggcgccggc 1140

gtaggcgttg gcttcgccga cctcccgggc gtgaagctgc ccaacttcgc ggcctgggga 1200

tccactgtgg gcgacgcggc cgcgtgcgag cagtcgtgcc tgggcaactg ctcgtgcggc 1260

gcgtacagct acagcactgg caccggctgc ctcacctggg gccaggacct gctggacatc 1320

taccgcttcc cggatggaga aggatacgat ctgcagatca aggtccccgc gtacttactt 1380

gagacgggct ccaaaaggag gcgatggacg acagttgtcg tcgccgtagt cgtcgccgtg 1440

gctgtattgg caggatgcgg ccttctcctg tggaaatgca ggagaaggat caaagagaaa 1500

cttggcatcg ttgttggcag tgaggagacg aaggcgacgc agccgtctct gcttcctttg 1560

agggaagcaa ggcaggattt ctcagggccg aagcagactg atcaagagga agcagagggc 1620

ggcaagaagt tcgagctgcc tatcttctcc ttggagacgg tagcggcggc caccggcgac 1680

ttcagtgccg acaacaagct tggggaggga ggctttggcc atgtctacaa gggaagactt 1740

cccggggccg aagaggttgc ggtgaagagg ctgtcccggg gctcagtgca ggggatggag 1800

gagtttaaga acgaggtcat cctgatcgcc aagctgcagc accgcaacct tgtcaagttg 1860

ttaggctgct gcatccaggg ggaggagaag atcttggtgt acgagtacat gcccaacaag 1920

agcctggacg gcttcctttt cgatccggcg cggcgaggcc tgctggactg gaagacgagg 1980

ttccacatca tcgagggcat cgcccgaggg ctcctgtacc tccacaggga ctcgcgtctc 2040

cgcgtggtgc accgcgacct caaggccagc aacatcctgc tagaccacga catgatcccc 2100

aagatctccg acttcggcat ggcgcgcatc ttcggcggcg accagaacca ggtgaacacg 2160

aaccgcgtcg tgggcacgct ggggtacatg tcacccgagt acgccatgga gggcctcttc 2220

tccgtgcgct ccgacgtgta cagcttcggc atcctcatcc tggagatcgt ctccgggcag 2280

aagaacagca gcttccacca catggagggc tccctcaaca tcgtcggcta cgcgtggcag 2340

ctgtggaacg ccgacagggg ggagcgcctg atcgacccgg ccatcctgcc ggcgtgctcc 2400

gtgcgggagg cgctgcgctg cgtgcacatg gcgctgctgt gcgtgcagga ccacgcgtgc 2460

gaccgcccgg acatccccta cgtcgtcatg gcgctcggca gcgacagctc cgtgctgccg 2520

atgcccaagc cgcccacgtt cacgctgcag tgcacgtcgt cgtcggacag ggacgggatc 2580

ttcccggaca aggtcgacga gtcctactcc gcctgcgatc tcaccgttac catgctgcat 2640

gggaggtag 2649

<210> 56

<211> 882

<212> PRT

<213> Zea mays

<400> 56

Met Ala His Ala Ala Ala Ala Ala Leu Ala Leu Leu Val Phe Phe Cys

1 5 10 15

Ala Gln Ala Arg Asp Ala Ala Val His Val Val Asp Ala Ala Ala Thr

20 25 30

Leu Ser Gln Gly Gln Ser Leu Gly Ala Thr Asp Lys Leu Val Ser Ala

35 40 45

Gly Gly Thr Phe Glu Leu Ala Phe Phe Thr Pro Thr Gly Gly Asp Pro

50 55 60

Ser Arg Arg Tyr Leu Gly Val Met Tyr Ala Gln Ser Thr Glu Gln Thr

65 70 75 80

Val Pro Trp Val Ala Asn Arg Asp Val Pro Val Ser Ala Gly Ser Ala

85 90 95

Tyr Ser Ala Thr Val Thr Ala Ala Gly Glu Leu Gln Val Leu Glu Gly

100 105 110

Asp Arg Val Val Trp Arg Thr Asp Asn Ser Ala Thr Thr Thr Ser Pro

115 120 125

Gly Thr Ala Gly Gly Glu Gln Ala Ala Asn Val Thr Leu Thr Val Leu

130 135 140

Asp Thr Gly Asn Leu Gln Leu Ala Ala Gly Asp Gly Gly Pro Val Ile

145 150 155 160

Trp Gln Ser Phe Asp His Pro Ala Asp Thr Phe Leu Pro Gly Met Ser

165 170 175

Ile Thr Leu Asp Arg Arg Gly Gly Gly Ala Val Arg Arg Thr Leu Phe

180 185 190

Thr Ser Trp Arg Ser Pro Ala Asp Pro Gly Thr Gly Asp Phe Thr Leu

195 200 205

Gly Gln Asp Pro Leu Gly Ser Ala Gln Leu Tyr Ile Trp Gln Thr Thr

210 215 220

Gly Gly Gln Asn Thr Thr Tyr Trp Arg Ser Gly Gln Trp Ala Asn Thr

225 230 235 240

Asn Phe Val Gly Val Pro Trp Arg Ser Leu Tyr Val Tyr Gly Phe Lys

245 250 255

Leu Asn Gly Asp Pro Asn Asn Gly Ser Gly Val Met Ser Tyr Val Phe

260 265 270

Asn Thr Tyr Asn Ser Ser Glu Tyr Arg Phe Met Leu His Ser Asn Gly

275 280 285

Thr Glu Thr Cys Tyr Met Leu Leu Ala Thr Gly Asp Trp Glu Thr Val

290 295 300

Trp Ser Gln Pro Thr Ile Pro Cys Gln Ala Tyr Asn Met Cys Gly Ala

305 310 315 320

Asn Ala Gln Cys Ala Ala Ala Ala Asp Gly Gly Gln Ala Val Cys Thr

325 330 335

Cys Leu Thr Gly Phe Glu Pro Arg Asn Val Ser Glu Tyr Ser Asn Gly

340 345 350

Asn Trp Thr Gln Gly Cys Val Arg Ser Ser Pro Leu Pro Cys Gly Gly

355 360 365

Glu Pro Asn Val Ser Gly Ala Gly Ala Gly Ala Gly Val Gly Val Gly

370 375 380

Phe Ala Asp Leu Pro Gly Val Lys Leu Pro Asn Phe Ala Ala Trp Gly

385 390 395 400

Ser Thr Val Gly Asp Ala Ala Ala Cys Glu Gln Ser Cys Leu Gly Asn

405 410 415

Cys Ser Cys Gly Ala Tyr Ser Tyr Ser Thr Gly Thr Gly Cys Leu Thr

420 425 430

Trp Gly Gln Asp Leu Leu Asp Ile Tyr Arg Phe Pro Asp Gly Glu Gly

435 440 445

Tyr Asp Leu Gln Ile Lys Val Pro Ala Tyr Leu Leu Glu Thr Gly Ser

450 455 460

Lys Arg Arg Arg Trp Thr Thr Val Val Val Ala Val Val Val Ala Val

465 470 475 480

Ala Val Leu Ala Gly Cys Gly Leu Leu Leu Trp Lys Cys Arg Arg Arg

485 490 495

Ile Lys Glu Lys Leu Gly Ile Val Val Gly Ser Glu Glu Thr Lys Ala

500 505 510

Thr Gln Pro Ser Leu Leu Pro Leu Arg Glu Ala Arg Gln Asp Phe Ser

515 520 525

Gly Pro Lys Gln Thr Asp Gln Glu Glu Ala Glu Gly Gly Lys Lys Phe

530 535 540

Glu Leu Pro Ile Phe Ser Leu Glu Thr Val Ala Ala Ala Thr Gly Asp

545 550 555 560

Phe Ser Ala Asp Asn Lys Leu Gly Glu Gly Gly Phe Gly His Val Tyr

565 570 575

Lys Gly Arg Leu Pro Gly Ala Glu Glu Val Ala Val Lys Arg Leu Ser

580 585 590

Arg Gly Ser Val Gln Gly Met Glu Glu Phe Lys Asn Glu Val Ile Leu

595 600 605

Ile Ala Lys Leu Gln His Arg Asn Leu Val Lys Leu Leu Gly Cys Cys

610 615 620

Ile Gln Gly Glu Glu Lys Ile Leu Val Tyr Glu Tyr Met Pro Asn Lys

625 630 635 640

Ser Leu Asp Gly Phe Leu Phe Asp Pro Ala Arg Arg Gly Leu Leu Asp

645 650 655

Trp Lys Thr Arg Phe His Ile Ile Glu Gly Ile Ala Arg Gly Leu Leu

660 665 670

Tyr Leu His Arg Asp Ser Arg Leu Arg Val Val His Arg Asp Leu Lys

675 680 685

Ala Ser Asn Ile Leu Leu Asp His Asp Met Ile Pro Lys Ile Ser Asp

690 695 700

Phe Gly Met Ala Arg Ile Phe Gly Gly Asp Gln Asn Gln Val Asn Thr

705 710 715 720

Asn Arg Val Val Gly Thr Leu Gly Tyr Met Ser Pro Glu Tyr Ala Met

725 730 735

Glu Gly Leu Phe Ser Val Arg Ser Asp Val Tyr Ser Phe Gly Ile Leu

740 745 750

Ile Leu Glu Ile Val Ser Gly Gln Lys Asn Ser Ser Phe His His Met

755 760 765

Glu Gly Ser Leu Asn Ile Val Gly Tyr Ala Trp Gln Leu Trp Asn Ala

770 775 780

Asp Arg Gly Glu Arg Leu Ile Asp Pro Ala Ile Leu Pro Ala Cys Ser

785 790 795 800

Val Arg Glu Ala Leu Arg Cys Val His Met Ala Leu Leu Cys Val Gln

805 810 815

Asp His Ala Cys Asp Arg Pro Asp Ile Pro Tyr Val Val Met Ala Leu

820 825 830

Gly Ser Asp Ser Ser Val Leu Pro Met Pro Lys Pro Pro Thr Phe Thr

835 840 845

Leu Gln Cys Thr Ser Ser Ser Asp Arg Asp Gly Ile Phe Pro Asp Lys

850 855 860

Val Asp Glu Ser Tyr Ser Ala Cys Asp Leu Thr Val Thr Met Leu His

865 870 875 880

Gly Arg

<210> 57

<211> 2640

<212> DNA

<213> Sorghum bicolor

<400> 57

atggcccacg ccgccgtggc agcgctcgcc ctgctcgtct tcttcagcgc gcaagcgcgg 60

cgggacggcg ccggcgtata cgtcgtggac gcggcggcgg cgctgtcgca ggggcactcg 120

ctgggcgccg gcgacaagct ggtgtcgtca gacggcacct tcgagctagc cttcttcacg 180

ccgaccgggg ccgccgaccc gtcgcggcgg tacctgggcg tcatgtacgc gcagtccaac 240

gagcagaccg tgccctgggt ggccaaccgc gacgcgcccg tgagcgccgg ctcgtcgtac 300

tcggccaccg tcacggacgc cggcgagctg caggtgcttg agggcgaacg cgtggtctgg 360

cgcaccaact ccgcgacgac ggcctcctcc tcctcctcct cgccggcgaa cgtgacgctc 420

accctcctcg acaccgggaa cctccagctg accgcgggcg caacggtgct ctggcagagc 480

ttcgaccacc ccgcggacac cttcctcccc gggatgagca tcacgctcga ccggaccaac 540

aggtccgccg tccgccggac gctgttcacg tcgtggcgga gccccgggga tcccggcacc 600

ggggacttca cgctggggca ggacccgctc ggctccgcgc agctctacat ctggcggacc 660

ggcggcgaga acaccaacag cacctactgg cggtccgggc agtgggccaa caccaacttc 720

gtgggcgtgc catggcggtc gctctacgtg tacggcttca agctcaacgg cgacccgtac 780

aacgactccg gtgtcatgtc ctacgtcttc aacacgtaca acagctccga gtaccggttc 840

atgctccact ccaacggcac cgagacgtgc tacatgctgc tcgacaccgg cgactgggag 900

accgtctggt cgcagccgac catcccgtgc caggcctaca acatgtgcgg cgccaacgcc 960

cggtgcgccg gcggcggcgg cggcgacgac ggccagcagg ccgtctgcac ctgcttgaca 1020

ggcttcgagc cgaggaatgt gtcggagtac ggcaacggga actggacgca gggatgcgtc 1080

aggagctccc cgctggcctg cagcagcgac gcgaacgtga gtggcggcgg cggcggcgat 1140

ggcttcgccg acctccccgg cgtgaagctg cccaacttcg cggcgtgggg atccacggtg 1200

ggcgacgcgg acgcgtgcaa gcagtcgtgc ctggccaact gctcgtgcgg cgcgtacagc 1260

tacagcggtg gcaccggctg cctcacctgg ggacaggacc tgctggacat ctaccagttc 1320

ccggatggag aaggatacga tctgcagatc aaggtccctg catacttact agatcagacg 1380

ggctccagaa gaaggcgatg gacgacagtt gccgttgctg tagttatcgt cgtggttgta 1440

ttggcaggat gcggccttct cctgtggaaa tgcaggagaa gaatcaaaga gaaacttggc 1500

attgttggta gggagaagac aaagacgaca acgcagccgt ctctgcttcc tttgagggaa 1560

gcacggcagg atttctcagg gccgaaacag gttgatcaag aggaagcaga gggcggcaag 1620

aagtgcgagc tgcctctctt ctccttggag atggttgcag cggccaccgg cgacttcagc 1680

gccgacaaca agcttggaga gggaggcttt ggccatgtct acaagggaag acttcccggt 1740

ggcgaagagg ttgcggtgaa gaggctgtcc cggggctcag ggcaggggct ggaggagttc 1800

aagaacgagg tgatcctgat cgccaagctg cagcaccgca accttgtgaa gttgctaggt 1860

tgctgcatcc agggggagga gaagatcctg gtctacgagt acatgcccaa caagagcctc 1920

gacgccttcc tcttcgatcc ggcgcggcga gggctgctgg actggaagac gaggttccac 1980

atcatcgagg gcatcgcccg tggcctcctg tacctccacc gtgactcgcg gctccgggtg 2040

gtgcaccggg acctcaaggc cagcaacatc ctgctggacc gcgacatgaa ccccaagatc 2100

tccgacttcg gcatggcgcg gatcttcggc ggcgaccaga accaggtgaa cacgaaccgc 2160

gtggtgggca cgctgggcta catgtcgccc gagtacgcca tggagggcct cttctccgtg 2220

cgctccgacg tctacagctt cggcatcctc atcctggaga tcgtctccgg gcagaagaac 2280

agcagcttcc accgcatgga gggctccctc aacatcgtcg gccacgcgtg gcagctctgg 2340

aacgccgaca gaggggagca gctcatcgac ccggccatcc tgccggcgtg ccccgtgcgg 2400

gaggcgctcc gctgcgtgca catggcgctg ctgtgcgtgc aggaccacgc gtgcgaccgc 2460

ccggacatct cctacgtcgt catggcgctc ggcagcgaca gctccgtgct gccgatgcct 2520

aagccgccga cgttcacgct gcagtgcacg tcgtcggaca gggacgggat cttcccggag 2580

agagtcgatg agtcctactc cgcctgtgac ctcaccgtca caatgctgca tgggaggtag 2640

<210> 58

<211> 879

<212> PRT

<213> Sorghum bicolor

<400> 58

Met Ala His Ala Ala Val Ala Ala Leu Ala Leu Leu Val Phe Phe Ser

1 5 10 15

Ala Gln Ala Arg Arg Asp Gly Ala Gly Val Tyr Val Val Asp Ala Ala

20 25 30

Ala Ala Leu Ser Gln Gly His Ser Leu Gly Ala Gly Asp Lys Leu Val

35 40 45

Ser Ser Asp Gly Thr Phe Glu Leu Ala Phe Phe Thr Pro Thr Gly Ala

50 55 60

Ala Asp Pro Ser Arg Arg Tyr Leu Gly Val Met Tyr Ala Gln Ser Asn

65 70 75 80

Glu Gln Thr Val Pro Trp Val Ala Asn Arg Asp Ala Pro Val Ser Ala

85 90 95

Gly Ser Ser Tyr Ser Ala Thr Val Thr Asp Ala Gly Glu Leu Gln Val

100 105 110

Leu Glu Gly Glu Arg Val Val Trp Arg Thr Asn Ser Ala Thr Thr Ala

115 120 125

Ser Ser Ser Ser Ser Ser Pro Ala Asn Val Thr Leu Thr Leu Leu Asp

130 135 140

Thr Gly Asn Leu Gln Leu Thr Ala Gly Ala Thr Val Leu Trp Gln Ser

145 150 155 160

Phe Asp His Pro Ala Asp Thr Phe Leu Pro Gly Met Ser Ile Thr Leu

165 170 175

Asp Arg Thr Asn Arg Ser Ala Val Arg Arg Thr Leu Phe Thr Ser Trp

180 185 190

Arg Ser Pro Gly Asp Pro Gly Thr Gly Asp Phe Thr Leu Gly Gln Asp

195 200 205

Pro Leu Gly Ser Ala Gln Leu Tyr Ile Trp Arg Thr Gly Gly Glu Asn

210 215 220

Thr Asn Ser Thr Tyr Trp Arg Ser Gly Gln Trp Ala Asn Thr Asn Phe

225 230 235 240

Val Gly Val Pro Trp Arg Ser Leu Tyr Val Tyr Gly Phe Lys Leu Asn

245 250 255

Gly Asp Pro Tyr Asn Asp Ser Gly Val Met Ser Tyr Val Phe Asn Thr

260 265 270

Tyr Asn Ser Ser Glu Tyr Arg Phe Met Leu His Ser Asn Gly Thr Glu

275 280 285

Thr Cys Tyr Met Leu Leu Asp Thr Gly Asp Trp Glu Thr Val Trp Ser

290 295 300

Gln Pro Thr Ile Pro Cys Gln Ala Tyr Asn Met Cys Gly Ala Asn Ala

305 310 315 320

Arg Cys Ala Gly Gly Gly Gly Gly Asp Asp Gly Gln Gln Ala Val Cys

325 330 335

Thr Cys Leu Thr Gly Phe Glu Pro Arg Asn Val Ser Glu Tyr Gly Asn

340 345 350

Gly Asn Trp Thr Gln Gly Cys Val Arg Ser Ser Pro Leu Ala Cys Ser

355 360 365

Ser Asp Ala Asn Val Ser Gly Gly Gly Gly Gly Asp Gly Phe Ala Asp

370 375 380

Leu Pro Gly Val Lys Leu Pro Asn Phe Ala Ala Trp Gly Ser Thr Val

385 390 395 400

Gly Asp Ala Asp Ala Cys Lys Gln Ser Cys Leu Ala Asn Cys Ser Cys

405 410 415

Gly Ala Tyr Ser Tyr Ser Gly Gly Thr Gly Cys Leu Thr Trp Gly Gln

420 425 430

Asp Leu Leu Asp Ile Tyr Gln Phe Pro Asp Gly Glu Gly Tyr Asp Leu

435 440 445

Gln Ile Lys Val Pro Ala Tyr Leu Leu Asp Gln Thr Gly Ser Arg Arg

450 455 460

Arg Arg Trp Thr Thr Val Ala Val Ala Val Val Ile Val Val Val Val

465 470 475 480

Leu Ala Gly Cys Gly Leu Leu Leu Trp Lys Cys Arg Arg Arg Ile Lys

485 490 495

Glu Lys Leu Gly Ile Val Gly Arg Glu Lys Thr Lys Thr Thr Thr Gln

500 505 510

Pro Ser Leu Leu Pro Leu Arg Glu Ala Arg Gln Asp Phe Ser Gly Pro

515 520 525

Lys Gln Val Asp Gln Glu Glu Ala Glu Gly Gly Lys Lys Cys Glu Leu

530 535 540

Pro Leu Phe Ser Leu Glu Met Val Ala Ala Ala Thr Gly Asp Phe Ser

545 550 555 560

Ala Asp Asn Lys Leu Gly Glu Gly Gly Phe Gly His Val Tyr Lys Gly

565 570 575

Arg Leu Pro Gly Gly Glu Glu Val Ala Val Lys Arg Leu Ser Arg Gly

580 585 590

Ser Gly Gln Gly Leu Glu Glu Phe Lys Asn Glu Val Ile Leu Ile Ala

595 600 605

Lys Leu Gln His Arg Asn Leu Val Lys Leu Leu Gly Cys Cys Ile Gln

610 615 620

Gly Glu Glu Lys Ile Leu Val Tyr Glu Tyr Met Pro Asn Lys Ser Leu

625 630 635 640

Asp Ala Phe Leu Phe Asp Pro Ala Arg Arg Gly Leu Leu Asp Trp Lys

645 650 655

Thr Arg Phe His Ile Ile Glu Gly Ile Ala Arg Gly Leu Leu Tyr Leu

660 665 670

His Arg Asp Ser Arg Leu Arg Val Val His Arg Asp Leu Lys Ala Ser

675 680 685

Asn Ile Leu Leu Asp Arg Asp Met Asn Pro Lys Ile Ser Asp Phe Gly

690 695 700

Met Ala Arg Ile Phe Gly Gly Asp Gln Asn Gln Val Asn Thr Asn Arg

705 710 715 720

Val Val Gly Thr Leu Gly Tyr Met Ser Pro Glu Tyr Ala Met Glu Gly

725 730 735

Leu Phe Ser Val Arg Ser Asp Val Tyr Ser Phe Gly Ile Leu Ile Leu

740 745 750

Glu Ile Val Ser Gly Gln Lys Asn Ser Ser Phe His Arg Met Glu Gly

755 760 765

Ser Leu Asn Ile Val Gly His Ala Trp Gln Leu Trp Asn Ala Asp Arg

770 775 780

Gly Glu Gln Leu Ile Asp Pro Ala Ile Leu Pro Ala Cys Pro Val Arg

785 790 795 800

Glu Ala Leu Arg Cys Val His Met Ala Leu Leu Cys Val Gln Asp His

805 810 815

Ala Cys Asp Arg Pro Asp Ile Ser Tyr Val Val Met Ala Leu Gly Ser

820 825 830

Asp Ser Ser Val Leu Pro Met Pro Lys Pro Pro Thr Phe Thr Leu Gln

835 840 845

Cys Thr Ser Ser Asp Arg Asp Gly Ile Phe Pro Glu Arg Val Asp Glu

850 855 860

Ser Tyr Ser Ala Cys Asp Leu Thr Val Thr Met Leu His Gly Arg

865 870 875

<210> 59

<211> 2523

<212> DNA

<213> Arabidopsis thaliana

<400> 59

atggtggttt cagtgaccat acgtcgtcgt tttgttcttc ttctacttgc atgcacttgt 60

ttgttgtctc ggagactctg ttttggtgag gacagaatca ctttctcaag tcctatcaaa 120

gactcagagt cagagaccct tctatgcaaa agtggtattt tcaggtttgg tttcttcact 180

cctgttaatt ccactactcg gttacgttat gttgggattt ggtacgaaaa gattccaatc 240

caaactgtgg tttgggttgc taacaaagac tctcccatca acgacacttc cggtgttatc 300

tcgatctatc aagatggaaa tctcgcggtt accgatggtc gaaatcgcct tgtatggtcg 360

acgaatgtct cagtaccagt agctccaaat gctacttggg ttcagcttat ggattctggg 420

aatcttatgt tacaagacaa cagaaacaat ggtgagattc tttgggagag tttcaagcat 480

ccttatgatt ctttcatgcc aagaatgact cttggcaccg acggtagaac tggaggtaac 540

ctaaagctta cttcttggac aagccacgat gatccttcaa caggaaacta cacagctggt 600

attgctcctt tcacgtttcc tgagcttctt atctggaaga acaatgtccc aacgtggcgt 660

agcggaccgt ggaacggtca ggttttcatc ggtttaccga atatggattc acttctgttt 720

cttgatgggt ttaatcttaa cagtgataat caaggaacga tctcaatgtc ttatgctaat 780

gattcgttca tgtatcactt taacttggat cctgaaggaa ttatttatca gaaagattgg 840

agtacttcta tgagaacttg gaggataggt gtaaagtttc catacacaga ctgtgatgca 900

tacggtagat gtggtcgatt cgggagctgc catgccgggg aaaacccgcc ttgtaaatgt 960

gttaaagggt ttgttccaaa gaataacaca gagtggaatg gtggtaattg gagtaatgga 1020

tgtatgagaa aagctccatt gcagtgtgaa agacagagaa atgtaagtaa tggtggtggt 1080

ggaggaaaag cagatggttt tttgaaactg cagaagatga aagtaccaat ctctgcggaa 1140

cggtctgaag ctagtgaaca agtttgtcct aaagtatgct tagataactg ttcttgcaca 1200

gcttatgcat atgatagagg aattggatgc atgctttgga gtggtgattt agttgatatg 1260

caatcatttt tgggaagtgg cattgatctt tttattcgtg ttgctcattc agaactcaaa 1320

acacatagca atctagcagt tatgatcgca gcacctgtga taggcgttat gttaattgct 1380

gcggtctgcg ttcttttagc atgccggaaa tacaaaaagc gtccagcgaa agatagaagt 1440

gcagagctaa tgtttaagag aatggaagca cttacaagtg ataatgagtc tgcttctaac 1500

caaatcaagc tcaaggagct tccactcttt gagtttcaag tgttagctac atcaactgat 1560

agcttctctc taagaaacaa gctcgggcaa ggcgggtttg gtcctgttta caagggaaaa 1620

ttacctgaag gacaagaaat tgcagtgaag aggctctcac ggaaatcagg tcaaggacta 1680

gaggaactta tgaacgaagt ggttgtgata tctaagttgc aacatcggaa tctagtgaag 1740

ttacttggat gttgtattga aggtgaagaa agaatgttag tatatgaata tatgccaaag 1800

aaaagcttgg atgcatatct ttttgaccca atgaagcaaa agattcttga ttggaagact 1860

cggttcaaca taatggaagg aatttgcaga ggtcttttgt accttcacag agattcaaga 1920

ctaaagatca tacacagaga tttaaaagcc agcaacattt tgttagatga gaatctaaac 1980

cccaagatat ctgattttgg acttgcaaga attttccgag cgaatgaaga tgaagctaac 2040

acaagaaggg ttgttggaac atacggctat atgtcaccgg agtatgcaat ggaaggtttc 2100

ttttcagaaa aatcagatgt tttcagcttg ggggttatat ttctcgagat cataagtggg 2160

agaagaaact ctagttctca caaggaagag aataatctca accttttggc ttatgcttgg 2220

aagctgtgga acgacggtga ggctgcttct ctagcagatc cagccgtctt tgataagtgt 2280

ttcgagaaag agatagagaa atgtgttcat attggactgt tatgtgtgca agaagttgca 2340

aacgatagac caaatgtttc aaacgtgata tggatgctaa ctaccgagaa catgagcctc 2400

gccgatccga agcagccagc gtttatagta agaagaggag cttctgaggc tgaatcttct 2460

gaccagagta gtcaaaaggt atctatcaat gatgtgagcc tcacagctgt aacaggacgt 2520

taa 2523

<210> 60

<211> 840

<212> PRT

<213> Arabidopsis thaliana

<400> 60

Met Val Val Ser Val Thr Ile Arg Arg Arg Phe Val Leu Leu Leu Leu

1 5 10 15

Ala Cys Thr Cys Leu Leu Ser Arg Arg Leu Cys Phe Gly Glu Asp Arg

20 25 30

Ile Thr Phe Ser Ser Pro Ile Lys Asp Ser Glu Ser Glu Thr Leu Leu

35 40 45

Cys Lys Ser Gly Ile Phe Arg Phe Gly Phe Phe Thr Pro Val Asn Ser

50 55 60

Thr Thr Arg Leu Arg Tyr Val Gly Ile Trp Tyr Glu Lys Ile Pro Ile

65 70 75 80

Gln Thr Val Val Trp Val Ala Asn Lys Asp Ser Pro Ile Asn Asp Thr

85 90 95

Ser Gly Val Ile Ser Ile Tyr Gln Asp Gly Asn Leu Ala Val Thr Asp

100 105 110

Gly Arg Asn Arg Leu Val Trp Ser Thr Asn Val Ser Val Pro Val Ala

115 120 125

Pro Asn Ala Thr Trp Val Gln Leu Met Asp Ser Gly Asn Leu Met Leu

130 135 140

Gln Asp Asn Arg Asn Asn Gly Glu Ile Leu Trp Glu Ser Phe Lys His

145 150 155 160

Pro Tyr Asp Ser Phe Met Pro Arg Met Thr Leu Gly Thr Asp Gly Arg

165 170 175

Thr Gly Gly Asn Leu Lys Leu Thr Ser Trp Thr Ser His Asp Asp Pro

180 185 190

Ser Thr Gly Asn Tyr Thr Ala Gly Ile Ala Pro Phe Thr Phe Pro Glu

195 200 205

Leu Leu Ile Trp Lys Asn Asn Val Pro Thr Trp Arg Ser Gly Pro Trp

210 215 220

Asn Gly Gln Val Phe Ile Gly Leu Pro Asn Met Asp Ser Leu Leu Phe

225 230 235 240

Leu Asp Gly Phe Asn Leu Asn Ser Asp Asn Gln Gly Thr Ile Ser Met

245 250 255

Ser Tyr Ala Asn Asp Ser Phe Met Tyr His Phe Asn Leu Asp Pro Glu

260 265 270

Gly Ile Ile Tyr Gln Lys Asp Trp Ser Thr Ser Met Arg Thr Trp Arg

275 280 285

Ile Gly Val Lys Phe Pro Tyr Thr Asp Cys Asp Ala Tyr Gly Arg Cys

290 295 300

Gly Arg Phe Gly Ser Cys His Ala Gly Glu Asn Pro Pro Cys Lys Cys

305 310 315 320

Val Lys Gly Phe Val Pro Lys Asn Asn Thr Glu Trp Asn Gly Gly Asn

325 330 335

Trp Ser Asn Gly Cys Met Arg Lys Ala Pro Leu Gln Cys Glu Arg Gln

340 345 350

Arg Asn Val Ser Asn Gly Gly Gly Gly Gly Lys Ala Asp Gly Phe Leu

355 360 365

Lys Leu Gln Lys Met Lys Val Pro Ile Ser Ala Glu Arg Ser Glu Ala

370 375 380

Ser Glu Gln Val Cys Pro Lys Val Cys Leu Asp Asn Cys Ser Cys Thr

385 390 395 400

Ala Tyr Ala Tyr Asp Arg Gly Ile Gly Cys Met Leu Trp Ser Gly Asp

405 410 415

Leu Val Asp Met Gln Ser Phe Leu Gly Ser Gly Ile Asp Leu Phe Ile

420 425 430

Arg Val Ala His Ser Glu Leu Lys Thr His Ser Asn Leu Ala Val Met

435 440 445

Ile Ala Ala Pro Val Ile Gly Val Met Leu Ile Ala Ala Val Cys Val

450 455 460

Leu Leu Ala Cys Arg Lys Tyr Lys Lys Arg Pro Ala Lys Asp Arg Ser

465 470 475 480

Ala Glu Leu Met Phe Lys Arg Met Glu Ala Leu Thr Ser Asp Asn Glu

485 490 495

Ser Ala Ser Asn Gln Ile Lys Leu Lys Glu Leu Pro Leu Phe Glu Phe

500 505 510

Gln Val Leu Ala Thr Ser Thr Asp Ser Phe Ser Leu Arg Asn Lys Leu

515 520 525

Gly Gln Gly Gly Phe Gly Pro Val Tyr Lys Gly Lys Leu Pro Glu Gly

530 535 540

Gln Glu Ile Ala Val Lys Arg Leu Ser Arg Lys Ser Gly Gln Gly Leu

545 550 555 560

Glu Glu Leu Met Asn Glu Val Val Val Ile Ser Lys Leu Gln His Arg

565 570 575

Asn Leu Val Lys Leu Leu Gly Cys Cys Ile Glu Gly Glu Glu Arg Met

580 585 590

Leu Val Tyr Glu Tyr Met Pro Lys Lys Ser Leu Asp Ala Tyr Leu Phe

595 600 605

Asp Pro Met Lys Gln Lys Ile Leu Asp Trp Lys Thr Arg Phe Asn Ile

610 615 620

Met Glu Gly Ile Cys Arg Gly Leu Leu Tyr Leu His Arg Asp Ser Arg

625 630 635 640

Leu Lys Ile Ile His Arg Asp Leu Lys Ala Ser Asn Ile Leu Leu Asp

645 650 655

Glu Asn Leu Asn Pro Lys Ile Ser Asp Phe Gly Leu Ala Arg Ile Phe

660 665 670

Arg Ala Asn Glu Asp Glu Ala Asn Thr Arg Arg Val Val Gly Thr Tyr

675 680 685

Gly Tyr Met Ser Pro Glu Tyr Ala Met Glu Gly Phe Phe Ser Glu Lys

690 695 700

Ser Asp Val Phe Ser Leu Gly Val Ile Phe Leu Glu Ile Ile Ser Gly

705 710 715 720

Arg Arg Asn Ser Ser Ser His Lys Glu Glu Asn Asn Leu Asn Leu Leu

725 730 735

Ala Tyr Ala Trp Lys Leu Trp Asn Asp Gly Glu Ala Ala Ser Leu Ala

740 745 750

Asp Pro Ala Val Phe Asp Lys Cys Phe Glu Lys Glu Ile Glu Lys Cys

755 760 765

Val His Ile Gly Leu Leu Cys Val Gln Glu Val Ala Asn Asp Arg Pro

770 775 780

Asn Val Ser Asn Val Ile Trp Met Leu Thr Thr Glu Asn Met Ser Leu

785 790 795 800

Ala Asp Pro Lys Gln Pro Ala Phe Ile Val Arg Arg Gly Ala Ser Glu

805 810 815

Ala Glu Ser Ser Asp Gln Ser Ser Gln Lys Val Ser Ile Asn Asp Val

820 825 830

Ser Leu Thr Ala Val Thr Gly Arg

835 840

<210> 61

<211> 2226

<212> DNA

<213> Glycine max

<400> 61

ttgctatcct tgtttcctac agctttggaa gcagaggatg caataactcc accacaaacc 60

atcagtggct accagacact agtctcacct tcccaaaatt ttgaactggg tttcttcagt 120

cctggcaatt ccactcacat ctatcttgga atatggtaca agcatatccc aaaacagaca 180

gttatttggg ttgcaaatag agacaaacca ttagttaact ctggtggctc cttaacattc 240

agcaacaatg gcaagctcat ccttctcagc cacacaggaa gtgttgtatg gtcttcaaac 300

tcatctggac ctgcaagaaa cccagtggca catcttctag actctggaaa ctttgtgttg 360

aaggactacg gaaatgaggg acatttgtgg gagagttttg attatccatc tgacacattg 420

ataccaggaa tgaagcttgg ttggaacttc aaaactggct tgaaccgaca tttgacttcc 480

tggaaaagct cttctaatcc ttcttcaggt gaatacactt acggtgtgga tccccggggg 540

attcctcagc tttttcttca caagggaaac aagaaggtct tcagaagtgg accatggtat 600

gggcaacaat tcaaaggtga tccagttctc agtgcaaatc cagttttcaa acccattttt 660

gtttttgatt ctgatgaagt gtcttactct tatgagacca aagacaccat tgtttcaaga 720

tttgtgctga gtcagtctgg tttgattcag catttttcat ggaatgatca tcattccagt 780

tggttctcag aattctcagt tcaaggggac cgctgtgatg attatggcct ttgtggtgca 840

tatggttctt gtaacatcaa aagctcccct gtttgcaaat gtttgaaggg ttttgatcct 900

aagcttccgc aagaatggga gaagaatgag tggtcaggtg gatgtgttag gaagaattca 960

caggttttca gcaatggaga cacatttaag cagtttacag gaatgaaact accagatgct 1020

gctgagtttc atacaaacta caccatcagc agtgatcact gtgaggcaga atgctccatg 1080

aactgctctt gtgttgctta tgccaaacta gatgtaaatg caagcggcaa aggctgtatt 1140

gtatggtttg gggatttatt tgatataaga gaggtttctg tgaatggaga agatttttac 1200

gtaagagttc cagcttcaga agtagccaaa gagacagata gtcagtttag tgttggcaga 1260

gctaggagtg aaagaaatga atttaaactc ccattgtttg agattgccat aattgaagcc 1320

gcaaccgaga atttctctct ttacaacaag attggagaag gaggatttgg tcatgtatac 1380

aaaggtcaac ttccatcagg acaggaaata gctgtgaaga ggctgtcaga gaattctgga 1440

caaggcctgc aggagttcaa gaatgaggtc attttgatct cccaacttca gcatcgaaat 1500

cttgtcaagc ttctgggatg ttgcattcat ggagaagata aaatgttggt ctacgaatac 1560

atgccaaaca gaagcttgga ctccttatta tttgatgaaa ccaagcgttc tgtgctcagt 1620

tggcaaaaga ggctagacat aattatcggc attgctcggg gacttctcta ccttcacaga 1680

gattcaagat tgagaataat ccatagagac ctgaaagcaa gcaatgttct tttagatggt 1740

gaaatgaacc caaaaatttc tgactttgga atggcaagaa tgtttggggg tgatcaaact 1800

gaagcaaaga ccaagagaat agtgggaacc tatggatata tgtccccaga gtatgcaata 1860

gatggacact tctctttcaa atcagatgtt tatagctttg gggttttact cttggagctg 1920

ttgagtggca agaaaaacaa aggatttatt cacccagatc acaaactgaa tctcctaggc 1980

catgcatgga agctatggaa tgaagatagg gccttggagc tgatggatgc attgcttgag 2040

aatcagtttc ctacatctga agctctaagg tgtattcaag tgggtctgtc atgcattcaa 2100

cagcacccag aagataggcc aacaatgtca tcagtgcttt tgatgttcga cagtgagagt 2160

gtgttggtcc cccaacctgg aaggccagga ttatattccg agagattttt ctctgggaca 2220

aattca 2226

<210> 62

<211> 742

<212> PRT

<213> Glycine max

<400> 62

Leu Leu Ser Leu Phe Pro Thr Ala Leu Glu Ala Glu Asp Ala Ile Thr

1 5 10 15

Pro Pro Gln Thr Ile Ser Gly Tyr Gln Thr Leu Val Ser Pro Ser Gln

20 25 30

Asn Phe Glu Leu Gly Phe Phe Ser Pro Gly Asn Ser Thr His Ile Tyr

35 40 45

Leu Gly Ile Trp Tyr Lys His Ile Pro Lys Gln Thr Val Ile Trp Val

50 55 60

Ala Asn Arg Asp Lys Pro Leu Val Asn Ser Gly Gly Ser Leu Thr Phe

65 70 75 80

Ser Asn Asn Gly Lys Leu Ile Leu Leu Ser His Thr Gly Ser Val Val

85 90 95

Trp Ser Ser Asn Ser Ser Gly Pro Ala Arg Asn Pro Val Ala His Leu

100 105 110

Leu Asp Ser Gly Asn Phe Val Leu Lys Asp Tyr Gly Asn Glu Gly His

115 120 125

Leu Trp Glu Ser Phe Asp Tyr Pro Ser Asp Thr Leu Ile Pro Gly Met

130 135 140

Lys Leu Gly Trp Asn Phe Lys Thr Gly Leu Asn Arg His Leu Thr Ser

145 150 155 160

Trp Lys Ser Ser Ser Asn Pro Ser Ser Gly Glu Tyr Thr Tyr Gly Val

165 170 175

Asp Pro Arg Gly Ile Pro Gln Leu Phe Leu His Lys Gly Asn Lys Lys

180 185 190

Val Phe Arg Ser Gly Pro Trp Tyr Gly Gln Gln Phe Lys Gly Asp Pro

195 200 205

Val Leu Ser Ala Asn Pro Val Phe Lys Pro Ile Phe Val Phe Asp Ser

210 215 220

Asp Glu Val Ser Tyr Ser Tyr Glu Thr Lys Asp Thr Ile Val Ser Arg

225 230 235 240

Phe Val Leu Ser Gln Ser Gly Leu Ile Gln His Phe Ser Trp Asn Asp

245 250 255

His His Ser Ser Trp Phe Ser Glu Phe Ser Val Gln Gly Asp Arg Cys

260 265 270

Asp Asp Tyr Gly Leu Cys Gly Ala Tyr Gly Ser Cys Asn Ile Lys Ser

275 280 285

Ser Pro Val Cys Lys Cys Leu Lys Gly Phe Asp Pro Lys Leu Pro Gln

290 295 300

Glu Trp Glu Lys Asn Glu Trp Ser Gly Gly Cys Val Arg Lys Asn Ser

305 310 315 320

Gln Val Phe Ser Asn Gly Asp Thr Phe Lys Gln Phe Thr Gly Met Lys

325 330 335

Leu Pro Asp Ala Ala Glu Phe His Thr Asn Tyr Thr Ile Ser Ser Asp

340 345 350

His Cys Glu Ala Glu Cys Ser Met Asn Cys Ser Cys Val Ala Tyr Ala

355 360 365

Lys Leu Asp Val Asn Ala Ser Gly Lys Gly Cys Ile Val Trp Phe Gly

370 375 380

Asp Leu Phe Asp Ile Arg Glu Val Ser Val Asn Gly Glu Asp Phe Tyr

385 390 395 400

Val Arg Val Pro Ala Ser Glu Val Ala Lys Glu Thr Asp Ser Gln Phe

405 410 415

Ser Val Gly Arg Ala Arg Ser Glu Arg Asn Glu Phe Lys Leu Pro Leu

420 425 430

Phe Glu Ile Ala Ile Ile Glu Ala Ala Thr Glu Asn Phe Ser Leu Tyr

435 440 445

Asn Lys Ile Gly Glu Gly Gly Phe Gly His Val Tyr Lys Gly Gln Leu

450 455 460

Pro Ser Gly Gln Glu Ile Ala Val Lys Arg Leu Ser Glu Asn Ser Gly

465 470 475 480

Gln Gly Leu Gln Glu Phe Lys Asn Glu Val Ile Leu Ile Ser Gln Leu

485 490 495

Gln His Arg Asn Leu Val Lys Leu Leu Gly Cys Cys Ile His Gly Glu

500 505 510

Asp Lys Met Leu Val Tyr Glu Tyr Met Pro Asn Arg Ser Leu Asp Ser

515 520 525

Leu Leu Phe Asp Glu Thr Lys Arg Ser Val Leu Ser Trp Gln Lys Arg

530 535 540

Leu Asp Ile Ile Ile Gly Ile Ala Arg Gly Leu Leu Tyr Leu His Arg

545 550 555 560

Asp Ser Arg Leu Arg Ile Ile His Arg Asp Leu Lys Ala Ser Asn Val

565 570 575

Leu Leu Asp Gly Glu Met Asn Pro Lys Ile Ser Asp Phe Gly Met Ala

580 585 590

Arg Met Phe Gly Gly Asp Gln Thr Glu Ala Lys Thr Lys Arg Ile Val

595 600 605

Gly Thr Tyr Gly Tyr Met Ser Pro Glu Tyr Ala Ile Asp Gly His Phe

610 615 620

Ser Phe Lys Ser Asp Val Tyr Ser Phe Gly Val Leu Leu Leu Glu Leu

625 630 635 640

Leu Ser Gly Lys Lys Asn Lys Gly Phe Ile His Pro Asp His Lys Leu

645 650 655

Asn Leu Leu Gly His Ala Trp Lys Leu Trp Asn Glu Asp Arg Ala Leu

660 665 670

Glu Leu Met Asp Ala Leu Leu Glu Asn Gln Phe Pro Thr Ser Glu Ala

675 680 685

Leu Arg Cys Ile Gln Val Gly Leu Ser Cys Ile Gln Gln His Pro Glu

690 695 700

Asp Arg Pro Thr Met Ser Ser Val Leu Leu Met Phe Asp Ser Glu Ser

705 710 715 720

Val Leu Val Pro Gln Pro Gly Arg Pro Gly Leu Tyr Ser Glu Arg Phe

725 730 735

Phe Ser Gly Thr Asn Ser

740

<210> 63

<211> 987

<212> DNA

<213> Oryza sativa

<400> 63

atgcagagtt acagtgagaa gagatctcat aagatatttt tggaaagcct tctcgtgtgt 60

ggaatttgtc taagtgagga tgttggcaga aatttcatca agctcccatg ccatcattcc 120

ttttgtttga agtgtatgga gtctcactgc aaaatccatg tgaaagaagg gaatttaatg 180

cagctggcat gtcctgatac aaattgccgc aatccacttc cgccatccgt attgaaaagc 240

cttctacgag atgatggata tgcacaatgg gaatcatttg ctctacagaa actgttagat 300

gcaatgcctg atctagttta ctgccccagg tgttctgctg cttgcttgga agttgacaat 360

gatgctcaat gcccgggttg tttttttacc ttctgcactt tgtgcaaacg ccgccgccat 420

gtgggggata catgtattac tcctgaagaa aaaatacgca ttttgaagga acgacagaaa 480

ttgtattcca taccagagga acaattgttg aaagaaaaga gggaaataga cgagttgata 540

aatatccagg aagcacttcg tgattctaag caatgccctc gttgtaagat ggctatctca 600

aaaattgaag gttgcaacaa gatgacatgt gggaactgtg ggagattctt ctgctatcgt 660

tgtaacaaag caattggagg atatgatcat ttctggaatg gaaactgtga tatgtttgag 720

agggaacaag atgaaaaccc acagcagcag gatgatgaaa attttggtgg cgaccctgac 780

gaagatgctg aactcttaga acctgaatgg gtgctgttaa cttatccctg tccaaactgt 840

ggccgtcgga atgaaaagct cggtaccaac aatcatatac tttgcatcgg atgccgaggc 900

cattattgtg cgttgtgtcg gaaaagagtt ttgaggggcg agcaacactt tggacctaga 960

ggctgccagc aacacactga agattga 987

<210> 64

<211> 328

<212> PRT

<213> Oryza sativa

<400> 64

Met Gln Ser Tyr Ser Glu Lys Arg Ser His Lys Ile Phe Leu Glu Ser

1 5 10 15

Leu Leu Val Cys Gly Ile Cys Leu Ser Glu Asp Val Gly Arg Asn Phe

20 25 30

Ile Lys Leu Pro Cys His His Ser Phe Cys Leu Lys Cys Met Glu Ser

35 40 45

His Cys Lys Ile His Val Lys Glu Gly Asn Leu Met Gln Leu Ala Cys

50 55 60

Pro Asp Thr Asn Cys Arg Asn Pro Leu Pro Pro Ser Val Leu Lys Ser

65 70 75 80

Leu Leu Arg Asp Asp Gly Tyr Ala Gln Trp Glu Ser Phe Ala Leu Gln

85 90 95

Lys Leu Leu Asp Ala Met Pro Asp Leu Val Tyr Cys Pro Arg Cys Ser

100 105 110

Ala Ala Cys Leu Glu Val Asp Asn Asp Ala Gln Cys Pro Gly Cys Phe

115 120 125

Phe Thr Phe Cys Thr Leu Cys Lys Arg Arg Arg His Val Gly Asp Thr

130 135 140

Cys Ile Thr Pro Glu Glu Lys Ile Arg Ile Leu Lys Glu Arg Gln Lys

145 150 155 160

Leu Tyr Ser Ile Pro Glu Glu Gln Leu Leu Lys Glu Lys Arg Glu Ile

165 170 175

Asp Glu Leu Ile Asn Ile Gln Glu Ala Leu Arg Asp Ser Lys Gln Cys

180 185 190

Pro Arg Cys Lys Met Ala Ile Ser Lys Ile Glu Gly Cys Asn Lys Met

195 200 205

Thr Cys Gly Asn Cys Gly Arg Phe Phe Cys Tyr Arg Cys Asn Lys Ala

210 215 220

Ile Gly Gly Tyr Asp His Phe Trp Asn Gly Asn Cys Asp Met Phe Glu

225 230 235 240

Arg Glu Gln Asp Glu Asn Pro Gln Gln Gln Asp Asp Glu Asn Phe Gly

245 250 255

Gly Asp Pro Asp Glu Asp Ala Glu Leu Leu Glu Pro Glu Trp Val Leu

260 265 270

Leu Thr Tyr Pro Cys Pro Asn Cys Gly Arg Arg Asn Glu Lys Leu Gly

275 280 285

Thr Asn Asn His Ile Leu Cys Ile Gly Cys Arg Gly His Tyr Cys Ala

290 295 300

Leu Cys Arg Lys Arg Val Leu Arg Gly Glu Gln His Phe Gly Pro Arg

305 310 315 320

Gly Cys Gln Gln His Thr Glu Asp

325

<210> 65

<211> 1686

<212> DNA

<213> Zea mays

<400> 65

atgtccagcg gagccacgtc atcgtcctcg ccgccgctgg cagaggctgg ggacggatac 60

tgggaggggc gggaggaggc ggtggcgcgg ctgaaggcga tggccgcgag ggctcattgg 120

gaggacgaac tctcggcgga gcggttggag accaacaacc aggtgcagga ggacgagatg 180

ctggcactgc aagccattta tggggatgac atggttatct tggaggataa ggctggtttt 240

cgttcttttc agatttttgt ttggtatcca attcctaatg gtactaaggt gttcttgaat 300

cttcatccaa atggaactat ggttggaact gataatgatg agagcaaaga tggtggtgaa 360

ctcatctatg cttgcagttt aaagcatttg ccacctgtgg tgttaacatg tttgctgcca 420

tgttcatatc ccagtgcaag tgctccttat tttactatct cagccaagtg gttggatgaa 480

cccaaagttt cacacctctg taccatgttt gatgagattt ggactgagct gccaggacaa 540

gaagtagtgt atagatggct ggactggctg aatagctctt catgggcttg catttctttg 600

aatgacaaca taatactaat cgcagataaa acttcagatg ttggagatgc acgtgctatt 660

gcaagaaggc ttctagttga tcatactatt cctcttatgc aaagttacaa tgagaggagg 720

tctcatgaaa tatttctgaa aagcttacat aagtgccgaa tttgtcttag tgaaaatact 780

ggcagaaatt tcatcaagct cccatgccac catttgtttt gcttgacgtg tatgaagtct 840

cactgcagaa ttcacgtaac agaagggagt ttaaccaagc tcacatgccc tgatacaact 900

tgctgcagcc cacttccacc atcagttttg aaaagcctcc tgggagatga ttgctataag 960

cgatgggaat cattcgcgtt acagaagctc ttagatacaa tgcctgatct ggtatattgt 1020

ccgaggtgta atgctgcctg cttggaggat gacaatgatg ctcaatgccc agagtgtttt 1080

ttcaccttct gctctttgtg caaagaacgc aggcatgtgg ggaaggattg cgttactcca 1140

gaagaaaaga ttaggattct gagggaaaaa caccagaaat attccctccc agaaaaacaa 1200

ttgttgaaag aacagaggga aatagatgaa ttaataagtg tctgcgaagc gcttcgcgat 1260

tcgaagcaat gtccttcttg caaaatggct atctcaaaga ctgaaggatg caacaagatg 1320

atctgtagga actgcgggaa attcttctgt taccgttgta accaggcaat tcgtggatat 1380

gaacattttt gggatggaaa ttgcgtgcta tttgaacacc agaatcaagt gcaaatatat 1440

ggacggcttg aggagtccga tgatgaagaa cattttgatg atgaagatct agaggagaca 1500

gagcctgaac tggtatgggg ttgtccttgt ccagtgtgtg gtcggtggaa tggaaagctt 1560

gacacaaaca atcatatatt gtgcatgggt tgccgaggcc actattgcgc gttatgccgg 1620

aggagggtaa tgaagagctc cgaacactat ggaccaagag gttgccaaca acacacggat 1680

ccgtag 1686

<210> 66

<211> 561

<212> PRT

<213> Zea mays

<400> 66

Met Ser Ser Gly Ala Thr Ser Ser Ser Ser Pro Pro Leu Ala Glu Ala

1 5 10 15

Gly Asp Gly Tyr Trp Glu Gly Arg Glu Glu Ala Val Ala Arg Leu Lys

20 25 30

Ala Met Ala Ala Arg Ala His Trp Glu Asp Glu Leu Ser Ala Glu Arg

35 40 45

Leu Glu Thr Asn Asn Gln Val Gln Glu Asp Glu Met Leu Ala Leu Gln

50 55 60

Ala Ile Tyr Gly Asp Asp Met Val Ile Leu Glu Asp Lys Ala Gly Phe

65 70 75 80

Arg Ser Phe Gln Ile Phe Val Trp Tyr Pro Ile Pro Asn Gly Thr Lys

85 90 95

Val Phe Leu Asn Leu His Pro Asn Gly Thr Met Val Gly Thr Asp Asn

100 105 110

Asp Glu Ser Lys Asp Gly Gly Glu Leu Ile Tyr Ala Cys Ser Leu Lys

115 120 125

His Leu Pro Pro Val Val Leu Thr Cys Leu Leu Pro Cys Ser Tyr Pro

130 135 140

Ser Ala Ser Ala Pro Tyr Phe Thr Ile Ser Ala Lys Trp Leu Asp Glu

145 150 155 160

Pro Lys Val Ser His Leu Cys Thr Met Phe Asp Glu Ile Trp Thr Glu

165 170 175

Leu Pro Gly Gln Glu Val Val Tyr Arg Trp Leu Asp Trp Leu Asn Ser

180 185 190

Ser Ser Trp Ala Cys Ile Ser Leu Asn Asp Asn Ile Ile Leu Ile Ala

195 200 205

Asp Lys Thr Ser Asp Val Gly Asp Ala Arg Ala Ile Ala Arg Arg Leu

210 215 220

Leu Val Asp His Thr Ile Pro Leu Met Gln Ser Tyr Asn Glu Arg Arg

225 230 235 240

Ser His Glu Ile Phe Leu Lys Ser Leu His Lys Cys Arg Ile Cys Leu

245 250 255

Ser Glu Asn Thr Gly Arg Asn Phe Ile Lys Leu Pro Cys His His Leu

260 265 270

Phe Cys Leu Thr Cys Met Lys Ser His Cys Arg Ile His Val Thr Glu

275 280 285

Gly Ser Leu Thr Lys Leu Thr Cys Pro Asp Thr Thr Cys Cys Ser Pro

290 295 300

Leu Pro Pro Ser Val Leu Lys Ser Leu Leu Gly Asp Asp Cys Tyr Lys

305 310 315 320

Arg Trp Glu Ser Phe Ala Leu Gln Lys Leu Leu Asp Thr Met Pro Asp

325 330 335

Leu Val Tyr Cys Pro Arg Cys Asn Ala Ala Cys Leu Glu Asp Asp Asn

340 345 350

Asp Ala Gln Cys Pro Glu Cys Phe Phe Thr Phe Cys Ser Leu Cys Lys

355 360 365

Glu Arg Arg His Val Gly Lys Asp Cys Val Thr Pro Glu Glu Lys Ile

370 375 380

Arg Ile Leu Arg Glu Lys His Gln Lys Tyr Ser Leu Pro Glu Lys Gln

385 390 395 400

Leu Leu Lys Glu Gln Arg Glu Ile Asp Glu Leu Ile Ser Val Cys Glu

405 410 415

Ala Leu Arg Asp Ser Lys Gln Cys Pro Ser Cys Lys Met Ala Ile Ser

420 425 430

Lys Thr Glu Gly Cys Asn Lys Met Ile Cys Arg Asn Cys Gly Lys Phe

435 440 445

Phe Cys Tyr Arg Cys Asn Gln Ala Ile Arg Gly Tyr Glu His Phe Trp

450 455 460

Asp Gly Asn Cys Val Leu Phe Glu His Gln Asn Gln Val Gln Ile Tyr

465 470 475 480

Gly Arg Leu Glu Glu Ser Asp Asp Glu Glu His Phe Asp Asp Glu Asp

485 490 495

Leu Glu Glu Thr Glu Pro Glu Leu Val Trp Gly Cys Pro Cys Pro Val

500 505 510

Cys Gly Arg Trp Asn Gly Lys Leu Asp Thr Asn Asn His Ile Leu Cys

515 520 525

Met Gly Cys Arg Gly His Tyr Cys Ala Leu Cys Arg Arg Arg Val Met

530 535 540

Lys Ser Ser Glu His Tyr Gly Pro Arg Gly Cys Gln Gln His Thr Asp

545 550 555 560

Pro

<210> 67

<211> 1809

<212> DNA

<213> Sorghum bicolor

<400> 67

atgtcgagcg gagcctcgtc atcgtcctcg ccgccgccgg cagagtctgg ggacggatac 60

tgggaggcgc gggaggaagc ggcggcgcgg ctggaggcga tggccgcgag ggctcgtggg 120

gaggacgagc tctcggcgga gcagttggag accaacaacc ggctccagga ggacgaggtt 180

gattatataa aactaaaaac gcttttctac aaaacacctg aggtcacgtg tgggcatgcg 240

gaggagccac agatgcctgg atgttctagt gcgagcaaac tcgaggtgct ggcactgcaa 300

gccatttatg gggatgacat ggtgatcttg gaggataagg catgtctacg ttcttttcag 360

ctttttgttc ggtatccaat tcctaatggc actaaggtgt tcttgaatct tcatccaaat 420

ggaactatgg ttggaactga taatgatggg agccaagatg gtagtgaact cttttatgct 480

tgcagcttaa agcatttgcc acctgtggtg ttaacttgtt tgctgccatg ttcatatccc 540

agtacaagtg ctccttattt tactatctca gccaagtggt tggatgaacc caaagtttca 600

cacctctgtg ccatgcttga tgagatttgg actgacctgc caggacaaga agtagtgtat 660

agatggctgg actggctgaa tagctcttca tggccttgca tttctttgaa tgacaacata 720

atactagtcc cagataaaac ttcagatgtt ggagatgaac gtgctattgc aagaaggctt 780

ctagttgatt ctactattcc tctgatgcaa agttacaatg agaggaggtc tcatgaaata 840

tttctgaaaa gctttcatga gtgtggaatt tgtcttagtg aaaatactgg cagaaatttc 900

atcaagctcc catgccacca tttgttttgc ttgacatgta tgaagtctca ctgcagaatt 960

cacgtaacag aagggaattt aacccagctg acatgccctg atacaacttg ccgcagccca 1020

ctcccaccat cagttttgaa aatccttctg ggagatgatt gctataagcg atgggaatcg 1080

ttcacattac agaaactctt agatacaatg cctgatctgg tatattgtcc aaggtgtgat 1140

gctgcctgct tggaggttga caatgatgct caatgccctg aatgtttttt caccttctgc 1200

tctttgtgca aagaacgccg gcatgtgggg gagcattgtg ttactccaga agaaaagatt 1260

aggattttga gggaaaaaca ccagaaatac tccctcccag aaaaacaatt gttgagagaa 1320

cagagggaaa tagatgaatt agtaaatgtc tgtgaagcgc ttcgcgattc taagcaatgt 1380

cccagatgta aaatggctat ctcgaaaact gaaggttgca acaagatgac ctgtaggaac 1440

tgtgggaaat tcttctgtta tcgttgtaac caggcaattc atggatatga acatttttgg 1500

gatggaaatt gtgtgctgtt tgaacatcac aatcaagtgg gaagaagata tggactgttt 1560

gaggaattgg atgacgatga aggttctgat gatgaagatc tagaggagcc agagccagag 1620

ccagagcctg aaatggtatg gggtcatcca tgtccaatgt gtggccggcg gaatgaaaag 1680

tttggcacaa acaatcatat attgtgcatg ggttgccgag gccactattg cgcgttatgc 1740

cggaagaggg ttacaaaaag ctcggaacac tatggaccaa gaggttgcca acaacacacg 1800

gatccctag 1809

<210> 68

<211> 602

<212> PRT

<213> Sorghum bicolor

<400> 68

Met Ser Ser Gly Ala Ser Ser Ser Ser Ser Pro Pro Pro Ala Glu Ser

1 5 10 15

Gly Asp Gly Tyr Trp Glu Ala Arg Glu Glu Ala Ala Ala Arg Leu Glu

20 25 30

Ala Met Ala Ala Arg Ala Arg Gly Glu Asp Glu Leu Ser Ala Glu Gln

35 40 45

Leu Glu Thr Asn Asn Arg Leu Gln Glu Asp Glu Val Asp Tyr Ile Lys

50 55 60

Leu Lys Thr Leu Phe Tyr Lys Thr Pro Glu Val Thr Cys Gly His Ala

65 70 75 80

Glu Glu Pro Gln Met Pro Gly Cys Ser Ser Ala Ser Lys Leu Glu Val

85 90 95

Leu Ala Leu Gln Ala Ile Tyr Gly Asp Asp Met Val Ile Leu Glu Asp

100 105 110

Lys Ala Cys Leu Arg Ser Phe Gln Leu Phe Val Arg Tyr Pro Ile Pro

115 120 125

Asn Gly Thr Lys Val Phe Leu Asn Leu His Pro Asn Gly Thr Met Val

130 135 140

Gly Thr Asp Asn Asp Gly Ser Gln Asp Gly Ser Glu Leu Phe Tyr Ala

145 150 155 160

Cys Ser Leu Lys His Leu Pro Pro Val Val Leu Thr Cys Leu Leu Pro

165 170 175

Cys Ser Tyr Pro Ser Thr Ser Ala Pro Tyr Phe Thr Ile Ser Ala Lys

180 185 190

Trp Leu Asp Glu Pro Lys Val Ser His Leu Cys Ala Met Leu Asp Glu

195 200 205

Ile Trp Thr Asp Leu Pro Gly Gln Glu Val Val Tyr Arg Trp Leu Asp

210 215 220

Trp Leu Asn Ser Ser Ser Trp Pro Cys Ile Ser Leu Asn Asp Asn Ile

225 230 235 240

Ile Leu Val Pro Asp Lys Thr Ser Asp Val Gly Asp Glu Arg Ala Ile

245 250 255

Ala Arg Arg Leu Leu Val Asp Ser Thr Ile Pro Leu Met Gln Ser Tyr

260 265 270

Asn Glu Arg Arg Ser His Glu Ile Phe Leu Lys Ser Phe His Glu Cys

275 280 285

Gly Ile Cys Leu Ser Glu Asn Thr Gly Arg Asn Phe Ile Lys Leu Pro

290 295 300

Cys His His Leu Phe Cys Leu Thr Cys Met Lys Ser His Cys Arg Ile

305 310 315 320

His Val Thr Glu Gly Asn Leu Thr Gln Leu Thr Cys Pro Asp Thr Thr

325 330 335

Cys Arg Ser Pro Leu Pro Pro Ser Val Leu Lys Ile Leu Leu Gly Asp

340 345 350

Asp Cys Tyr Lys Arg Trp Glu Ser Phe Thr Leu Gln Lys Leu Leu Asp

355 360 365

Thr Met Pro Asp Leu Val Tyr Cys Pro Arg Cys Asp Ala Ala Cys Leu

370 375 380

Glu Val Asp Asn Asp Ala Gln Cys Pro Glu Cys Phe Phe Thr Phe Cys

385 390 395 400

Ser Leu Cys Lys Glu Arg Arg His Val Gly Glu His Cys Val Thr Pro

405 410 415

Glu Glu Lys Ile Arg Ile Leu Arg Glu Lys His Gln Lys Tyr Ser Leu

420 425 430

Pro Glu Lys Gln Leu Leu Arg Glu Gln Arg Glu Ile Asp Glu Leu Val

435 440 445

Asn Val Cys Glu Ala Leu Arg Asp Ser Lys Gln Cys Pro Arg Cys Lys

450 455 460

Met Ala Ile Ser Lys Thr Glu Gly Cys Asn Lys Met Thr Cys Arg Asn

465 470 475 480

Cys Gly Lys Phe Phe Cys Tyr Arg Cys Asn Gln Ala Ile His Gly Tyr

485 490 495

Glu His Phe Trp Asp Gly Asn Cys Val Leu Phe Glu His His Asn Gln

500 505 510

Val Gly Arg Arg Tyr Gly Leu Phe Glu Glu Leu Asp Asp Asp Glu Gly

515 520 525

Ser Asp Asp Glu Asp Leu Glu Glu Pro Glu Pro Glu Pro Glu Pro Glu

530 535 540

Met Val Trp Gly His Pro Cys Pro Met Cys Gly Arg Arg Asn Glu Lys

545 550 555 560

Phe Gly Thr Asn Asn His Ile Leu Cys Met Gly Cys Arg Gly His Tyr

565 570 575

Cys Ala Leu Cys Arg Lys Arg Val Thr Lys Ser Ser Glu His Tyr Gly

580 585 590

Pro Arg Gly Cys Gln Gln His Thr Asp Pro

595 600

<210> 69

<211> 2067

<212> DNA

<213> Arabidopsis thaliana

<400> 69

atgagaggaa cacacggagg cagtggaaga aatcacgacc gccgctacat ccgccgtgac 60

gctgggattg tgaatccagt caacgatcat ccaaaatcag agcatcagaa agaagacacg 120

acttcgacgg atttatcatc tgcagcagct tttccgtcta cttctcagcc aatccaaaac 180

ctcgatcatt ctacaaaacc ttcaaagtct caccggaatc gacgcagccg tggatcaaat 240

tcaaaacctc gtcccgtgga gaaaccggag gtgaattttg ctgaatccga tgttgttgat 300

tgcttggctg atgaattgag taggttaaag gtgaaacaga attcgcattc ggtgaatatg 360

gaggagaagt ttcatcacag ttctagtgat cattcgaatt gtgaggaatc ggagttaaag 420

agagctgaaa gtgaagaaat tgctgatggt gttgatgaat atgagacgaa ggaagatatt 480

atgttgacaa ttctgaaaga tttgcgatct agtgtcagtg agccagagct aacagaggag 540

caactaaaga tgaatgatca attacaggaa gatgagcttt tggcattggg gtacatatat 600

ggaggaaaca tgttcatctt tgatcggtat aacgatatgc gatactttca ggttcatgta 660

aacgttgaag ctactagtga atatactatc tctacaaagc ttaacttaca agctgattca 720

agcaaagagt cagaggattt cttgtactct ttcaaggcgc aacaccttcc accgattgtg 780

ttgaaatgtt tattaccaaa agcttatccg agccacttac cgccttattt cttaataagt 840

gttcagtgga tgaatcctga taagatttca agtctctgct ctaagctgga ctcgttatgg 900

agtgaacagc cagggcaaga agtcttgtac cagtggacag attggctaca gaactcgtcg 960

atttctcatc ttggttttga taatgagatt gttcttggtc cttatggtgt tacatgttcc 1020

agagataagc gtgctgtgtc tggaagtcgt tctccggatt cagatattcc ttatattagg 1080

agttatgacg atgagaaacg tcatgatagc ttccttcaga gcttgcatga gtgctgcata 1140

tgcttctctg aatctgcagg tatcgacttt gttaagttac catgccagca tttcttttgt 1200

ttgaaatgta tgaagaccta tacagacata catgtcactg aaggcacagt taacaagctc 1260

aagtgtccgg actcgaaatg tggagaaact gttccgccag ggatattgaa gagattgttg 1320

ggcgatgaag cgtatgaacg gtgggaaact cttatgttac agaaaacact ggaatccatg 1380

actgacgttg cttactgtcc tagatgtgaa accccttgca tagaggacga ggaacagctt 1440

gctttatgct tcaaatgcta cttcagtttc tgtacacttt gtaaggaaaa gagacatgtg 1500

ggtgtggctt gcatgagccc ggaacttagg cttcaaatct tgcaggagcg tcaagattct 1560

tctcgtcttg gagaggaaca aaggcggaaa gagaaagaaa tgatcaatga gattatgagt 1620

gtgaaggtga taatgaaaag cgctaaacaa tgtccgtcgt gcaaaatagc catctcaaga 1680

accggaggtt gtaacaaaat ggtatgcaat aactgtggtc aatacttttg ttaccgttgc 1740

aacgaagcta tcactggtta tgaacatttc agtgagggaa aatgtgaact gttcccacaa 1800

gaggcgatcc aagaatggaa tgagatgatg aatgagagac aagtcattgg acagattcaa 1860

gcgcaactgt ttgcacaagg cggtcagttt ccacagcgcg gtcagttatg tcctaattgc 1920

cgccagttca atggaaaggc gggaaacaac aatcacttgt tttgttgggc atgtcaagca 1980

catttctgtt atctttgtaa gaaagtggtg aagaaatctg ctcagcatta tggaccaaag 2040

ggctgcaagc agcacacaga tgggtaa 2067

<210> 70

<211> 688

<212> PRT

<213> Arabidopsis thaliana

<400> 70

Met Arg Gly Thr His Gly Gly Ser Gly Arg Asn His Asp Arg Arg Tyr

1 5 10 15

Ile Arg Arg Asp Ala Gly Ile Val Asn Pro Val Asn Asp His Pro Lys

20 25 30

Ser Glu His Gln Lys Glu Asp Thr Thr Ser Thr Asp Leu Ser Ser Ala

35 40 45

Ala Ala Phe Pro Ser Thr Ser Gln Pro Ile Gln Asn Leu Asp His Ser

50 55 60

Thr Lys Pro Ser Lys Ser His Arg Asn Arg Arg Ser Arg Gly Ser Asn

65 70 75 80

Ser Lys Pro Arg Pro Val Glu Lys Pro Glu Val Asn Phe Ala Glu Ser

85 90 95

Asp Val Val Asp Cys Leu Ala Asp Glu Leu Ser Arg Leu Lys Val Lys

100 105 110

Gln Asn Ser His Ser Val Asn Met Glu Glu Lys Phe His His Ser Ser

115 120 125

Ser Asp His Ser Asn Cys Glu Glu Ser Glu Leu Lys Arg Ala Glu Ser

130 135 140

Glu Glu Ile Ala Asp Gly Val Asp Glu Tyr Glu Thr Lys Glu Asp Ile

145 150 155 160

Met Leu Thr Ile Leu Lys Asp Leu Arg Ser Ser Val Ser Glu Pro Glu

165 170 175

Leu Thr Glu Glu Gln Leu Lys Met Asn Asp Gln Leu Gln Glu Asp Glu

180 185 190

Leu Leu Ala Leu Gly Tyr Ile Tyr Gly Gly Asn Met Phe Ile Phe Asp

195 200 205

Arg Tyr Asn Asp Met Arg Tyr Phe Gln Val His Val Asn Val Glu Ala

210 215 220

Thr Ser Glu Tyr Thr Ile Ser Thr Lys Leu Asn Leu Gln Ala Asp Ser

225 230 235 240

Ser Lys Glu Ser Glu Asp Phe Leu Tyr Ser Phe Lys Ala Gln His Leu

245 250 255

Pro Pro Ile Val Leu Lys Cys Leu Leu Pro Lys Ala Tyr Pro Ser His

260 265 270

Leu Pro Pro Tyr Phe Leu Ile Ser Val Gln Trp Met Asn Pro Asp Lys

275 280 285

Ile Ser Ser Leu Cys Ser Lys Leu Asp Ser Leu Trp Ser Glu Gln Pro

290 295 300

Gly Gln Glu Val Leu Tyr Gln Trp Thr Asp Trp Leu Gln Asn Ser Ser

305 310 315 320

Ile Ser His Leu Gly Phe Asp Asn Glu Ile Val Leu Gly Pro Tyr Gly

325 330 335

Val Thr Cys Ser Arg Asp Lys Arg Ala Val Ser Gly Ser Arg Ser Pro

340 345 350

Asp Ser Asp Ile Pro Tyr Ile Arg Ser Tyr Asp Asp Glu Lys Arg His

355 360 365

Asp Ser Phe Leu Gln Ser Leu His Glu Cys Cys Ile Cys Phe Ser Glu

370 375 380

Ser Ala Gly Ile Asp Phe Val Lys Leu Pro Cys Gln His Phe Phe Cys

385 390 395 400

Leu Lys Cys Met Lys Thr Tyr Thr Asp Ile His Val Thr Glu Gly Thr

405 410 415

Val Asn Lys Leu Lys Cys Pro Asp Ser Lys Cys Gly Glu Thr Val Pro

420 425 430

Pro Gly Ile Leu Lys Arg Leu Leu Gly Asp Glu Ala Tyr Glu Arg Trp

435 440 445

Glu Thr Leu Met Leu Gln Lys Thr Leu Glu Ser Met Thr Asp Val Ala

450 455 460

Tyr Cys Pro Arg Cys Glu Thr Pro Cys Ile Glu Asp Glu Glu Gln Leu

465 470 475 480

Ala Leu Cys Phe Lys Cys Tyr Phe Ser Phe Cys Thr Leu Cys Lys Glu

485 490 495

Lys Arg His Val Gly Val Ala Cys Met Ser Pro Glu Leu Arg Leu Gln

500 505 510

Ile Leu Gln Glu Arg Gln Asp Ser Ser Arg Leu Gly Glu Glu Gln Arg

515 520 525

Arg Lys Glu Lys Glu Met Ile Asn Glu Ile Met Ser Val Lys Val Ile

530 535 540

Met Lys Ser Ala Lys Gln Cys Pro Ser Cys Lys Ile Ala Ile Ser Arg

545 550 555 560

Thr Gly Gly Cys Asn Lys Met Val Cys Asn Asn Cys Gly Gln Tyr Phe

565 570 575

Cys Tyr Arg Cys Asn Glu Ala Ile Thr Gly Tyr Glu His Phe Ser Glu

580 585 590

Gly Lys Cys Glu Leu Phe Pro Gln Glu Ala Ile Gln Glu Trp Asn Glu

595 600 605

Met Met Asn Glu Arg Gln Val Ile Gly Gln Ile Gln Ala Gln Leu Phe

610 615 620

Ala Gln Gly Gly Gln Phe Pro Gln Arg Gly Gln Leu Cys Pro Asn Cys

625 630 635 640

Arg Gln Phe Asn Gly Lys Ala Gly Asn Asn Asn His Leu Phe Cys Trp

645 650 655

Ala Cys Gln Ala His Phe Cys Tyr Leu Cys Lys Lys Val Val Lys Lys

660 665 670

Ser Ala Gln His Tyr Gly Pro Lys Gly Cys Lys Gln His Thr Asp Gly

675 680 685

<210> 71

<211> 1050

<212> DNA

<213> Glycine max

<400> 71

atgaattatg tccaggatga gcgtgttatt tcaggagctg aatgcattga tgttgatatt 60

ccttttttac gaagttacaa cgatgagaga cacaatgaga acttcctcaa agaattgcat 120

gattgtaaca tttgctttag tgaatatgca ggctctcagt tcatccggtt accatgtgag 180

catttttttt gcctcaaatg cttgcagacc tttgcccaga tacatgtaaa ggaaggcact 240

gttagtaatc ttaaatgtcc tgaagcaaaa tgtgcaatta tgattcctcc tggcctttta 300

aaacaattgc tggatgacac agattatgag cgctgggaat ccatgatgtt ggaaaaaaca 360

cttgcatcaa tgtctgatgt tgtttattgt ccaaggtgtg aaacaccctg catagaggat 420

gaagaccagc atgctcaatg cccaaaatgt tactttagct tttgtaccct ttgcagggaa 480

cgacgccatg ttggcatagc atgcatgagt ctagatatga agcttcagat tttgcaggac 540

cgtcaaaatt tgtctcaatt aaaggaagat caaaagcgaa gggaacgtga aaagatcaat 600

gaaatgctca atatgaaaga aattcatcgt gattccaagc tgtgcccttc ttgtgacatg 660

gcaatttctc gaactgaagg ttgtaacaaa atgaaatgtg gtaactgtga acaatacttc 720

tgttaccgct gcaacaaagc aattgatgca tcagatccat atggacattt cagggatggt 780

tcatgtgaat tgttcccacg agaaatggtt gattcctggg aagagcgcat taatcatcgc 840

caggcggtag gacaactaca ggctgagctc tttcctcaac atggcttggc gtgtcctagt 900

tgtcgtcaat ataatccaaa gattggaaat aataatcact tgttttgctg ggcatgccaa 960

cgccattact gctacttatg caaagcgatt gtcaggcgtg gcactaagca ttatggacca 1020

aagggctgca aacagcactc tgagggatag 1050

<210> 72

<211> 349

<212> PRT

<213> Glycine max

<400> 72

Met Asn Tyr Val Gln Asp Glu Arg Val Ile Ser Gly Ala Glu Cys Ile

1 5 10 15

Asp Val Asp Ile Pro Phe Leu Arg Ser Tyr Asn Asp Glu Arg His Asn

20 25 30

Glu Asn Phe Leu Lys Glu Leu His Asp Cys Asn Ile Cys Phe Ser Glu

35 40 45

Tyr Ala Gly Ser Gln Phe Ile Arg Leu Pro Cys Glu His Phe Phe Cys

50 55 60

Leu Lys Cys Leu Gln Thr Phe Ala Gln Ile His Val Lys Glu Gly Thr

65 70 75 80

Val Ser Asn Leu Lys Cys Pro Glu Ala Lys Cys Ala Ile Met Ile Pro

85 90 95

Pro Gly Leu Leu Lys Gln Leu Leu Asp Asp Thr Asp Tyr Glu Arg Trp

100 105 110

Glu Ser Met Met Leu Glu Lys Thr Leu Ala Ser Met Ser Asp Val Val

115 120 125

Tyr Cys Pro Arg Cys Glu Thr Pro Cys Ile Glu Asp Glu Asp Gln His

130 135 140

Ala Gln Cys Pro Lys Cys Tyr Phe Ser Phe Cys Thr Leu Cys Arg Glu

145 150 155 160

Arg Arg His Val Gly Ile Ala Cys Met Ser Leu Asp Met Lys Leu Gln

165 170 175

Ile Leu Gln Asp Arg Gln Asn Leu Ser Gln Leu Lys Glu Asp Gln Lys

180 185 190

Arg Arg Glu Arg Glu Lys Ile Asn Glu Met Leu Asn Met Lys Glu Ile

195 200 205

His Arg Asp Ser Lys Leu Cys Pro Ser Cys Asp Met Ala Ile Ser Arg

210 215 220

Thr Glu Gly Cys Asn Lys Met Lys Cys Gly Asn Cys Glu Gln Tyr Phe

225 230 235 240

Cys Tyr Arg Cys Asn Lys Ala Ile Asp Ala Ser Asp Pro Tyr Gly His

245 250 255

Phe Arg Asp Gly Ser Cys Glu Leu Phe Pro Arg Glu Met Val Asp Ser

260 265 270

Trp Glu Glu Arg Ile Asn His Arg Gln Ala Val Gly Gln Leu Gln Ala

275 280 285

Glu Leu Phe Pro Gln His Gly Leu Ala Cys Pro Ser Cys Arg Gln Tyr

290 295 300

Asn Pro Lys Ile Gly Asn Asn Asn His Leu Phe Cys Trp Ala Cys Gln

305 310 315 320

Arg His Tyr Cys Tyr Leu Cys Lys Ala Ile Val Arg Arg Gly Thr Lys

325 330 335

His Tyr Gly Pro Lys Gly Cys Lys Gln His Ser Glu Gly

340 345

<210> 73

<211> 303

<212> DNA

<213> Oryza sativa

<400> 73

atgtcggccg ccggggagga ggacaagaag ccggcggggg gagagggcgg cggcgcccac 60

atcaacctca aggtcaaggg acaggatggg aacgaggtat tcttccgcat caagagatct 120

acgcagctga agaagctgat gaacgcctat tgtgaccgtc agtctgtgga tatgaatgct 180

attgcattcc tatttgatgg tcgtaggctc cgtggcgagc agacccctga cgagctcgag 240

atggaagacg gggacgagat cgacgccatg ctccaccaga ctggaggctg cctgcctgcc 300

tag 303

<210> 74

<211> 100

<212> PRT

<213> Oryza sativa

<400> 74

Met Ser Ala Ala Gly Glu Glu Asp Lys Lys Pro Ala Gly Gly Glu Gly

1 5 10 15

Gly Gly Ala His Ile Asn Leu Lys Val Lys Gly Gln Asp Gly Asn Glu

20 25 30

Val Phe Phe Arg Ile Lys Arg Ser Thr Gln Leu Lys Lys Leu Met Asn

35 40 45

Ala Tyr Cys Asp Arg Gln Ser Val Asp Met Asn Ala Ile Ala Phe Leu

50 55 60

Phe Asp Gly Arg Arg Leu Arg Gly Glu Gln Thr Pro Asp Glu Leu Glu

65 70 75 80

Met Glu Asp Gly Asp Glu Ile Asp Ala Met Leu His Gln Thr Gly Gly

85 90 95

Cys Leu Pro Ala

100

<210> 75

<211> 300

<212> DNA

<213> Zea mays

<400> 75

atgtctggag ccggcgagga ggacaagaag cccgcggagg gcggcgccca catcaacctt 60

aaggtcaagg gacaggatgg caatgaggtg ttctttcgca taaagaggtc cacccagctg 120

aagaagctga tgaacgctta ttgtgaccgc cagtctgtgg acatgaatgc cattgcgttc 180

ctgtttgatg gccgcaggct tcgcggcgag cagacccctg atgagctgga gatggaggac 240

ggcgacgaga tcgatgccat gcttcaccag accggaggca gcgttcctag caccacctaa 300

<210> 76

<211> 99

<212> PRT

<213> Zea mays

<400> 76

Met Ser Gly Ala Gly Glu Glu Asp Lys Lys Pro Ala Glu Gly Gly Ala

1 5 10 15

His Ile Asn Leu Lys Val Lys Gly Gln Asp Gly Asn Glu Val Phe Phe

20 25 30

Arg Ile Lys Arg Ser Thr Gln Leu Lys Lys Leu Met Asn Ala Tyr Cys

35 40 45

Asp Arg Gln Ser Val Asp Met Asn Ala Ile Ala Phe Leu Phe Asp Gly

50 55 60

Arg Arg Leu Arg Gly Glu Gln Thr Pro Asp Glu Leu Glu Met Glu Asp

65 70 75 80

Gly Asp Glu Ile Asp Ala Met Leu His Gln Thr Gly Gly Ser Val Pro

85 90 95

Ser Thr Thr

<210> 77

<211> 300

<212> DNA

<213> Sorghum bicolor

<400> 77

atgtctggag ccggtgagga ggacaagaag cccgcggagg cgggcggcgc ccacatcaat 60

ctcaaggtca agggacagga tggcaatgag gttttcttcc gcattaagag gtccacccag 120

ctgaagaagc tgatgaacgc ctactgtgac cgccagtctg tggatatgaa tgccattgca 180

ttcctgtttg atggccgcag gcttcgcggc gagcagaccc cggatgagct ggagatggaa 240

gacggcgatg agattgacgc catgcttcac cagactggag gcagcgttcc tggcgcctag 300

<210> 78

<211> 99

<212> PRT

<213> Sorghum bicolor

<400> 78

Met Ser Gly Ala Gly Glu Glu Asp Lys Lys Pro Ala Glu Ala Gly Gly

1 5 10 15

Ala His Ile Asn Leu Lys Val Lys Gly Gln Asp Gly Asn Glu Val Phe

20 25 30

Phe Arg Ile Lys Arg Ser Thr Gln Leu Lys Lys Leu Met Asn Ala Tyr

35 40 45

Cys Asp Arg Gln Ser Val Asp Met Asn Ala Ile Ala Phe Leu Phe Asp

50 55 60

Gly Arg Arg Leu Arg Gly Glu Gln Thr Pro Asp Glu Leu Glu Met Glu

65 70 75 80

Asp Gly Asp Glu Ile Asp Ala Met Leu His Gln Thr Gly Gly Ser Val

85 90 95

Pro Gly Ala

<210> 79

<211> 303

<212> DNA

<213> Arabidopsis thaliana

<400> 79

atgtctgcaa accaggagga agacaagaag ccaggagacg gaggagctca catcaatctc 60

aaagtcaagg gacaggatgg aaacgaggtt ttctttagga tcaagagaag cactcagctc 120

aagaagctga tgaatgctta ctgtgaccgg caatctgtgg acatgaactc cattgctttc 180

ttgtttgatg ggcgtcgtct tcgtgctgag caaactcccg atgagcttga catggaggat 240

ggtgatgaga tcgatgcgat gctccatcag actggtggca gcggtggtgg tgctacggcc 300

tga 303

<210> 80

<211> 100

<212> PRT

<213> Arabidopsis thaliana

<400> 80

Met Ser Ala Asn Gln Glu Glu Asp Lys Lys Pro Gly Asp Gly Gly Ala

1 5 10 15

His Ile Asn Leu Lys Val Lys Gly Gln Asp Gly Asn Glu Val Phe Phe

20 25 30

Arg Ile Lys Arg Ser Thr Gln Leu Lys Lys Leu Met Asn Ala Tyr Cys

35 40 45

Asp Arg Gln Ser Val Asp Met Asn Ser Ile Ala Phe Leu Phe Asp Gly

50 55 60

Arg Arg Leu Arg Ala Glu Gln Thr Pro Asp Glu Leu Asp Met Glu Asp

65 70 75 80

Gly Asp Glu Ile Asp Ala Met Leu His Gln Thr Gly Gly Ser Gly Gly

85 90 95

Gly Ala Thr Ala

100

<210> 81

<211> 300

<212> DNA

<213> Glycine max

<400> 81

atgtcgggcg ttaccaacaa caacgaggaa gacaagaagc caaccgaaca gggagcacac 60

atcaacctta aggttaaggg gcaggatggg aatgaagttt ttttcaggat caagagaagc 120

actcaattga agaagcttat gaatgcttac tgtgaccgac agtcggtgga tttcaactcc 180

attgcttttc tgtttgatgg acgccgcctc cgagctgagc agactccaga tgagttggaa 240

atggaggacg gggacgagat tgatgccatg cttcaccaga caggaggttc tgttgtctga 300

<210> 82

<211> 99

<212> PRT

<213> Glycine max

<400> 82

Met Ser Gly Val Thr Asn Asn Asn Glu Glu Asp Lys Lys Pro Thr Glu

1 5 10 15

Gln Gly Ala His Ile Asn Leu Lys Val Lys Gly Gln Asp Gly Asn Glu

20 25 30

Val Phe Phe Arg Ile Lys Arg Ser Thr Gln Leu Lys Lys Leu Met Asn

35 40 45

Ala Tyr Cys Asp Arg Gln Ser Val Asp Phe Asn Ser Ile Ala Phe Leu

50 55 60

Phe Asp Gly Arg Arg Leu Arg Ala Glu Gln Thr Pro Asp Glu Leu Glu

65 70 75 80

Met Glu Asp Gly Asp Glu Ile Asp Ala Met Leu His Gln Thr Gly Gly

85 90 95

Ser Val Val

<210> 83

<211> 2154

<212> DNA

<213> Oryza sativa

<400> 83

atggcggacg ccgccgccgc cgccgccggc ggcggaggca gccaccgttc cgtccaccat 60

gcgcacctcg cggcgctgct caacccctcc ccgcgctcgc cgccgcaccc gcacccgctc 120

cagctccacc ggcgccacct cccgctgtcc cttcccgcgg cccgccgcct cgccgcggcc 180

ttcccgccgc tcccgctcct cctctccctc ctcgccgcgc tccgcctcct cccctcgccg 240

ccgccgccgc gccccttcga cgcgctcatc aggtcgtacg cctccctccc tcgcccctcc 300

ctcgccgccg cggcgctcgc gttcgccgcg tcggccgggt acgccccctc cgtcccggcc 360

tacaacgccg tcctcctcgc gctctccgac gcctcgctcc cctccgcccg ccgcttcctc 420

tcctccatgc tccgccacgg cgtggcgccc aacgtgtaca cctacaacat cctcgtccgc 480

gcgctctgcg cccgcggccg cctggaggag gcggtcggcg tcgtgggcga catgcgtggc 540

gcgggctgcg cgcccaacgc cgtcacctac aacacgctcg tcgcggcgtt ctgcagggcc 600

ggggagttgg acggcgcgga gagggtggtc agcttgatga gggaggaggg caatgcgaag 660

ccgaatctgg tgaccttcaa ctcgatggtg aatgggctgt gtaaagcggg gaggatggag 720

ggtgcacgca aggtgttcga tgaaatggtg agggaggggt tggcaccgga tgtggtcagc 780

tacaacacct tgttaagtgg ttactgcaag gtgggttgct tacatgagtc attggcggtg 840

ttttcagaga tgacacagag agggcttgtg ccagatgtcg tgacgtttac gtcgctaatc 900

catgcaacat gtaaggctgg taacttggag caggcagtgg ctctggtggc gcagatgagg 960

gagaggggcc ttcggatgaa cgaggtcact ttcacggcac ttattgatgg tttctgcaag 1020

aaggggttct tggatgatgc attgcttgca gttgaggaga tgaggaaatg tgggatccag 1080

ccttcagtgg tatgttacaa tgcacttatt aatgggtact gcaagttagg aagaatggat 1140

ctagcaagag agctaatccg tgaaatggag gctaaaagag tgaaacccga tgttgtgacc 1200

tatagtacga ttatcagcgg atactgtaag gttggcaatt tggattctgc attccaattg 1260

aatcagaaaa tgctcaaaaa gggtgtgctt ccagatgcaa tcacctactc atcactcata 1320

aggggtcttt gtgaagagaa aaggctcaat gatgcttgtg agctatttga gaacatgctt 1380

caacttggtg tgcaacctga cgaatttact tatacaacac tcattgatgg ccactgcaag 1440

gaaggaaatg ttgagaaggc tctttctctg catgatgaga tgataaggaa gggagttctc 1500

cctgatgttg tgacgtacag tgtgctcata aatgggctta gcaagtcagc acgtacaaag 1560

gaggcacatc ggttactctt taaactgtat catgaagatc cggttccaga caatattaag 1620

tatgatgctc tgatgctttg ttgcagcaaa gctgagttta agagtgtagt ggccctcctg 1680

aagggatttt gcatgaaagg cttgatgaag gaagctgata aagtatacca gtcgatgttg 1740

gacagaaact ggaagcttga tgggtctgtg tacagtatac ttattcatgg gcattgcagg 1800

ggaggaaatg ttaggaaggc tctcagcttt cataaacaaa tgttgcggag tgggttttcc 1860

cctaattcaa caagcaccat ttcattggtt aggggtttat ttgaagaggg gatggttgtg 1920

gaagcagata atgccattca ggacttactg acttgttgtc cactagcaga tgcagaagca 1980

tcaaaggctc ttattgacct caatcggaag gaagggatgg actacttcca agctcagggt 2040

gaaatctggt attcagaatc ctccccctct gtggaatact ggaattacat gatgaccata 2100

tgtcactttg gagtttggag cacttatttt tccatgcttc gcttaacaaa atga 2154

<210> 84

<211> 717

<212> PRT

<213> Oryza sativa

<400> 84

Met Ala Asp Ala Ala Ala Ala Ala Ala Gly Gly Gly Gly Ser His Arg

1 5 10 15

Ser Val His His Ala His Leu Ala Ala Leu Leu Asn Pro Ser Pro Arg

20 25 30

Ser Pro Pro His Pro His Pro Leu Gln Leu His Arg Arg His Leu Pro

35 40 45

Leu Ser Leu Pro Ala Ala Arg Arg Leu Ala Ala Ala Phe Pro Pro Leu

50 55 60

Pro Leu Leu Leu Ser Leu Leu Ala Ala Leu Arg Leu Leu Pro Ser Pro

65 70 75 80

Pro Pro Pro Arg Pro Phe Asp Ala Leu Ile Arg Ser Tyr Ala Ser Leu

85 90 95

Pro Arg Pro Ser Leu Ala Ala Ala Ala Leu Ala Phe Ala Ala Ser Ala

100 105 110

Gly Tyr Ala Pro Ser Val Pro Ala Tyr Asn Ala Val Leu Leu Ala Leu

115 120 125

Ser Asp Ala Ser Leu Pro Ser Ala Arg Arg Phe Leu Ser Ser Met Leu

130 135 140

Arg His Gly Val Ala Pro Asn Val Tyr Thr Tyr Asn Ile Leu Val Arg

145 150 155 160

Ala Leu Cys Ala Arg Gly Arg Leu Glu Glu Ala Val Gly Val Val Gly

165 170 175

Asp Met Arg Gly Ala Gly Cys Ala Pro Asn Ala Val Thr Tyr Asn Thr

180 185 190

Leu Val Ala Ala Phe Cys Arg Ala Gly Glu Leu Asp Gly Ala Glu Arg

195 200 205

Val Val Ser Leu Met Arg Glu Glu Gly Asn Ala Lys Pro Asn Leu Val

210 215 220

Thr Phe Asn Ser Met Val Asn Gly Leu Cys Lys Ala Gly Arg Met Glu

225 230 235 240

Gly Ala Arg Lys Val Phe Asp Glu Met Val Arg Glu Gly Leu Ala Pro

245 250 255

Asp Val Val Ser Tyr Asn Thr Leu Leu Ser Gly Tyr Cys Lys Val Gly

260 265 270

Cys Leu His Glu Ser Leu Ala Val Phe Ser Glu Met Thr Gln Arg Gly

275 280 285

Leu Val Pro Asp Val Val Thr Phe Thr Ser Leu Ile His Ala Thr Cys

290 295 300

Lys Ala Gly Asn Leu Glu Gln Ala Val Ala Leu Val Ala Gln Met Arg

305 310 315 320

Glu Arg Gly Leu Arg Met Asn Glu Val Thr Phe Thr Ala Leu Ile Asp

325 330 335

Gly Phe Cys Lys Lys Gly Phe Leu Asp Asp Ala Leu Leu Ala Val Glu

340 345 350

Glu Met Arg Lys Cys Gly Ile Gln Pro Ser Val Val Cys Tyr Asn Ala

355 360 365

Leu Ile Asn Gly Tyr Cys Lys Leu Gly Arg Met Asp Leu Ala Arg Glu

370 375 380

Leu Ile Arg Glu Met Glu Ala Lys Arg Val Lys Pro Asp Val Val Thr

385 390 395 400

Tyr Ser Thr Ile Ile Ser Gly Tyr Cys Lys Val Gly Asn Leu Asp Ser

405 410 415

Ala Phe Gln Leu Asn Gln Lys Met Leu Lys Lys Gly Val Leu Pro Asp

420 425 430

Ala Ile Thr Tyr Ser Ser Leu Ile Arg Gly Leu Cys Glu Glu Lys Arg

435 440 445

Leu Asn Asp Ala Cys Glu Leu Phe Glu Asn Met Leu Gln Leu Gly Val

450 455 460

Gln Pro Asp Glu Phe Thr Tyr Thr Thr Leu Ile Asp Gly His Cys Lys

465 470 475 480

Glu Gly Asn Val Glu Lys Ala Leu Ser Leu His Asp Glu Met Ile Arg

485 490 495

Lys Gly Val Leu Pro Asp Val Val Thr Tyr Ser Val Leu Ile Asn Gly

500 505 510

Leu Ser Lys Ser Ala Arg Thr Lys Glu Ala His Arg Leu Leu Phe Lys

515 520 525

Leu Tyr His Glu Asp Pro Val Pro Asp Asn Ile Lys Tyr Asp Ala Leu

530 535 540

Met Leu Cys Cys Ser Lys Ala Glu Phe Lys Ser Val Val Ala Leu Leu

545 550 555 560

Lys Gly Phe Cys Met Lys Gly Leu Met Lys Glu Ala Asp Lys Val Tyr

565 570 575

Gln Ser Met Leu Asp Arg Asn Trp Lys Leu Asp Gly Ser Val Tyr Ser

580 585 590

Ile Leu Ile His Gly His Cys Arg Gly Gly Asn Val Arg Lys Ala Leu

595 600 605

Ser Phe His Lys Gln Met Leu Arg Ser Gly Phe Ser Pro Asn Ser Thr

610 615 620

Ser Thr Ile Ser Leu Val Arg Gly Leu Phe Glu Glu Gly Met Val Val

625 630 635 640

Glu Ala Asp Asn Ala Ile Gln Asp Leu Leu Thr Cys Cys Pro Leu Ala

645 650 655

Asp Ala Glu Ala Ser Lys Ala Leu Ile Asp Leu Asn Arg Lys Glu Gly

660 665 670

Met Asp Tyr Phe Gln Ala Gln Gly Glu Ile Trp Tyr Ser Glu Ser Ser

675 680 685

Pro Ser Val Glu Tyr Trp Asn Tyr Met Met Thr Ile Cys His Phe Gly

690 695 700

Val Trp Ser Thr Tyr Phe Ser Met Leu Arg Leu Thr Lys

705 710 715

<210> 85

<211> 1950

<212> DNA

<213> Zea mays

<400> 85

atggcgatcc cccgtcgcct atcttgcctt cccacggttg ccgcggccac caagtggtcg 60

gccgggaatc tcgccataga catcggcaca atcggcgcga gaccctcaac ggcagccctg 120

gctgccgccg cgacggcggc cgccgccgcc ggccgcgcct cggagtgcca gtccctcctc 180

ctccgcatgt cgcgccgccg cggcgcctct cgacgcgaga tcgtctcttc cctcctcgcc 240

tcgtccccca ctccgcagcc gcaggtgttc gacctcctca tccgcaccta cacccagtcg 300

cggaagcccc gggaggcctt tgaggccttc cgcctcctcc tcgaccaccg cgtccccgtc 360

ccagcctctg cgtccaacgc tctccttgcc gtgctctccc gcgccgggtg gcctcacctc 420

gcccaggagg cctaccgcct cgtcctctcg tctgactcgg aggtaaacgc ctacacgctt 480

aacatcatgg tccacagcta ctgtaaaacc ctagagttcg atggggctga cactgtcatc 540

tccgagatgg agaagagatg cgtgttcccg gatgtggtta cacacaatgt gttgattgat 600

gccagattcc gtgctggaga tgtggatgca gcgatcgcat tggttgattc catggccaat 660

agggggttga agcctgggat tgtgacatac aattcggttt tgaaggggtt gtgtaagcac 720

agaaggtttg ataaggcaaa ggaggtgttc aggacaatgg accagtgcag tgttgcacca 780

gatgttcgga gtttcaatat tttgatcgga ggattttgta gggttgggga ggttaaggag 840

gcagtgaagt tttacaagga gatgcaacat cgttatgtta caccagatgt ggtcagcttt 900

agctgtttga ttggattatt ctcgaggaga ggggagatgg accatgcagg ggcatacttg 960

agggaaatga aggggcttgg attggtgcct gatggtgtga tttacacaat ggtcataggt 1020

gggttttgta gggctggatc aatgtcagag gctctaagag ttagggatga gatggttggc 1080

tttggatgtc tgccagatgt ggtaacatac aataccctgt tgaatgggct ctgtaagcag 1140

cacaggttgc ttgacgcgga gaaacttttg aatgagatgg aggagagagg ggttacacca 1200

gatttatgca ctttcacaac tttgattcat gggtactgta ggcaaggtaa ctttgagaat 1260

gcattgcaat tgtttgacac actgttgcgc cagcgtttga ggccagatgt cgtgacatac 1320

aatagtttga ttgatgggat gtgcagaaaa ggtgatctgg ccaaagctaa tgagctatgg 1380

gatgatatgc atgctcgaga aatcttaccc aatcacatta cctacagtat cctaatagac 1440

agccactgtg agaagggaca agttgaggat gcattcggtt ttctggatga aatggtcaag 1500

aagggaaatt tgcctaatat caggacctat aattccatca tcaagggtta ttgccgatca 1560

ggtaatgtaa aaaaggggca gcagtttttg caaaagatga ggcaagataa cgtattccca 1620

gatctgatta ctttcaacac cctgatccat ggttatataa aagaagaaaa catgcatgga 1680

gctttcaatg tgtttaatat aatggagaag gaaatggtac ggccagatgc tgtcacgtat 1740

aatatgatca taaatgggtt ttctgaacaa ggcaatatgc aagatgctgg tcgtgttttc 1800

aagggaatgg gtgacagtgg aattgaaccg gatagatata catacatgtc attgataaat 1860

ggtcatgtta cagctggcaa ctcgaagcag gcgttccaac ttcatgacga gatgatccat 1920

agagggtttg ctcccgatga caaattttga 1950

<210> 86

<211> 649

<212> PRT

<213> Zea mays

<400> 86

Met Ala Ile Pro Arg Arg Leu Ser Cys Leu Pro Thr Val Ala Ala Ala

1 5 10 15

Thr Lys Trp Ser Ala Gly Asn Leu Ala Ile Asp Ile Gly Thr Ile Gly

20 25 30

Ala Arg Pro Ser Thr Ala Ala Leu Ala Ala Ala Ala Thr Ala Ala Ala

35 40 45

Ala Ala Gly Arg Ala Ser Glu Cys Gln Ser Leu Leu Leu Arg Met Ser

50 55 60

Arg Arg Arg Gly Ala Ser Arg Arg Glu Ile Val Ser Ser Leu Leu Ala

65 70 75 80

Ser Ser Pro Thr Pro Gln Pro Gln Val Phe Asp Leu Leu Ile Arg Thr

85 90 95

Tyr Thr Gln Ser Arg Lys Pro Arg Glu Ala Phe Glu Ala Phe Arg Leu

100 105 110

Leu Leu Asp His Arg Val Pro Val Pro Ala Ser Ala Ser Asn Ala Leu

115 120 125

Leu Ala Val Leu Ser Arg Ala Gly Trp Pro His Leu Ala Gln Glu Ala

130 135 140

Tyr Arg Leu Val Leu Ser Ser Asp Ser Glu Val Asn Ala Tyr Thr Leu

145 150 155 160

Asn Ile Met Val His Ser Tyr Cys Lys Thr Leu Glu Phe Asp Gly Ala

165 170 175

Asp Thr Val Ile Ser Glu Met Glu Lys Arg Cys Val Phe Pro Asp Val

180 185 190

Val Thr His Asn Val Leu Ile Asp Ala Arg Phe Arg Ala Gly Asp Val

195 200 205

Asp Ala Ala Ile Ala Leu Val Asp Ser Met Ala Asn Arg Gly Leu Lys

210 215 220

Pro Gly Ile Val Thr Tyr Asn Ser Val Leu Lys Gly Leu Cys Lys His

225 230 235 240

Arg Arg Phe Asp Lys Ala Lys Glu Val Phe Arg Thr Met Asp Gln Cys

245 250 255

Ser Val Ala Pro Asp Val Arg Ser Phe Asn Ile Leu Ile Gly Gly Phe

260 265 270

Cys Arg Val Gly Glu Val Lys Glu Ala Val Lys Phe Tyr Lys Glu Met

275 280 285

Gln His Arg Tyr Val Thr Pro Asp Val Val Ser Phe Ser Cys Leu Ile

290 295 300

Gly Leu Phe Ser Arg Arg Gly Glu Met Asp His Ala Gly Ala Tyr Leu

305 310 315 320

Arg Glu Met Lys Gly Leu Gly Leu Val Pro Asp Gly Val Ile Tyr Thr

325 330 335

Met Val Ile Gly Gly Phe Cys Arg Ala Gly Ser Met Ser Glu Ala Leu

340 345 350

Arg Val Arg Asp Glu Met Val Gly Phe Gly Cys Leu Pro Asp Val Val

355 360 365

Thr Tyr Asn Thr Leu Leu Asn Gly Leu Cys Lys Gln His Arg Leu Leu

370 375 380

Asp Ala Glu Lys Leu Leu Asn Glu Met Glu Glu Arg Gly Val Thr Pro

385 390 395 400

Asp Leu Cys Thr Phe Thr Thr Leu Ile His Gly Tyr Cys Arg Gln Gly

405 410 415

Asn Phe Glu Asn Ala Leu Gln Leu Phe Asp Thr Leu Leu Arg Gln Arg

420 425 430

Leu Arg Pro Asp Val Val Thr Tyr Asn Ser Leu Ile Asp Gly Met Cys

435 440 445

Arg Lys Gly Asp Leu Ala Lys Ala Asn Glu Leu Trp Asp Asp Met His

450 455 460

Ala Arg Glu Ile Leu Pro Asn His Ile Thr Tyr Ser Ile Leu Ile Asp

465 470 475 480

Ser His Cys Glu Lys Gly Gln Val Glu Asp Ala Phe Gly Phe Leu Asp

485 490 495

Glu Met Val Lys Lys Gly Asn Leu Pro Asn Ile Arg Thr Tyr Asn Ser

500 505 510

Ile Ile Lys Gly Tyr Cys Arg Ser Gly Asn Val Lys Lys Gly Gln Gln

515 520 525

Phe Leu Gln Lys Met Arg Gln Asp Asn Val Phe Pro Asp Leu Ile Thr

530 535 540

Phe Asn Thr Leu Ile His Gly Tyr Ile Lys Glu Glu Asn Met His Gly

545 550 555 560

Ala Phe Asn Val Phe Asn Ile Met Glu Lys Glu Met Val Arg Pro Asp

565 570 575

Ala Val Thr Tyr Asn Met Ile Ile Asn Gly Phe Ser Glu Gln Gly Asn

580 585 590

Met Gln Asp Ala Gly Arg Val Phe Lys Gly Met Gly Asp Ser Gly Ile

595 600 605

Glu Pro Asp Arg Tyr Thr Tyr Met Ser Leu Ile Asn Gly His Val Thr

610 615 620

Ala Gly Asn Ser Lys Gln Ala Phe Gln Leu His Asp Glu Met Ile His

625 630 635 640

Arg Gly Phe Ala Pro Asp Asp Lys Phe

645

<210> 87

<211> 1950

<212> DNA

<213> Sorghum bicolor

<400> 87

atggcgattc cccggcgcct ctttcgcctt cccgcggccg ccgcagccac caattggtcg 60

gccggaactc tcgccgcaga catcggcgga accggcgcga gaccctcaac ggccgccctg 120

gctgccgcag cgacggacgc cgccgccgcc ggccgcgcct cggagtgcca gtccctcctc 180

ctccgcatgt cgcgccgccg cggtgcctcc cgacgcgaga tcgtctcctc cctcctcgcc 240

tcgtccccca ccccacagcc gcaggtgttc gacctcctca tccgcaccta cacccagtcc 300

cgcaagcccc gggaggcctt cgaggccttc cgcctcctcc ttgaccaccg cgtccccgtc 360

cccgcctccg cgtccaacgc cctccttgcc gcgctctccc gggccgggtg gcctcacctc 420

gccgaggagg cctaccgcct cgtcctctcg tccgactctg aggtaaacgc ctacacgctt 480

aacatcatgg tccacagcta ctgtaaaagc ctagagttcg ataaggctga cactgtcatc 540

tccgagatgg agaagagatg cgtgtttccg gatgtggtta cacataatgt gttgattgat 600

gccagattcc gtgccggaga tgtggatgca gcaattgcat tggttgattc gatggctaat 660

aaggggttga agcctgggat tgtgaccttc aattcggttt tgaaggggtt gtgtaagcac 720

aggagattcg ataaggcaaa ggaggtgttc agggcaatgg accagtgcag tgttgcgcca 780

gatgttcgga gtttcaatat tttgatcgga ggattttgta gggttgggga ggttgaggag 840

gcgatgaagt tttacaagga gatgcaacag cgtggtgtta caccagatgt ggtcagcttt 900

agctgtctga tcggattatt ctcaacgagg gggaagatgg accatgcggc agcatacttg 960

agggaaatga aggggcttgg gttggtgcca gatggtgtga tttacacaat ggtcataggt 1020

gggttttgta gggctggatc aatgtcagag gctctacgag ttagggatga gatggttggc 1080

cttggatgtc tgccagatgt cgtaacatac aataccctgt tgaatgggct ctgtaagcag 1140

cacaggttgc ttgacgcaga ggagcttttg aatgagatga aggagagagg ggttacacca 1200

gatttatgca ctttcacaac tttgattcat gggtattgta gggatggtaa ttttgagaaa 1260

gcattgcaat tgtttgacac actgttgcac cagcgtttga ggccagatgt cgtggcatac 1320

aatagcttga ttgatggaat gtgcagaaaa ggtgatctgg ccaaagctaa tgagctatgg 1380

gatgatatgc atgctcgaga aatatttccc aatcatgtta cctacagcat cctaatagac 1440

agccactgtg agaagggaca agttgaagaa gcattcggtt ttctggatga aatggtcagt 1500

aagggcaatt taccaaacat catgacctat aattccatca tcaagggtta ttgccgatca 1560

ggtaatgtaa aaaaggggca gcagtttttg caaaagatga tgcaagataa catattgcca 1620

gatctgatta ctttcaacac cctgatccat ggttatataa aagaagaaaa catgcatgga 1680

gctttcaatg tgtttaatat aatggagaag gaaatggtac aaccagatgc tgtcacatat 1740

aatatgatca taaatgggtt ttctgaacaa ggcaatatgg aagaagctgg ccgtgttttc 1800

aagaaaatgg gtgccagtgg aattgaaccg gatagatata catacatgtc tctgataaat 1860

ggtcatgtta cagctggcaa ctcgaaggag gcattccagc ttcatgatga gatgatgcat 1920

agagggtttg ctcctgacga caaattctga 1950

<210> 88

<211> 649

<212> PRT

<213> Sorghum bicolor

<400> 88

Met Ala Ile Pro Arg Arg Leu Phe Arg Leu Pro Ala Ala Ala Ala Ala

1 5 10 15

Thr Asn Trp Ser Ala Gly Thr Leu Ala Ala Asp Ile Gly Gly Thr Gly

20 25 30

Ala Arg Pro Ser Thr Ala Ala Leu Ala Ala Ala Ala Thr Asp Ala Ala

35 40 45

Ala Ala Gly Arg Ala Ser Glu Cys Gln Ser Leu Leu Leu Arg Met Ser

50 55 60

Arg Arg Arg Gly Ala Ser Arg Arg Glu Ile Val Ser Ser Leu Leu Ala

65 70 75 80

Ser Ser Pro Thr Pro Gln Pro Gln Val Phe Asp Leu Leu Ile Arg Thr

85 90 95

Tyr Thr Gln Ser Arg Lys Pro Arg Glu Ala Phe Glu Ala Phe Arg Leu

100 105 110

Leu Leu Asp His Arg Val Pro Val Pro Ala Ser Ala Ser Asn Ala Leu

115 120 125

Leu Ala Ala Leu Ser Arg Ala Gly Trp Pro His Leu Ala Glu Glu Ala

130 135 140

Tyr Arg Leu Val Leu Ser Ser Asp Ser Glu Val Asn Ala Tyr Thr Leu

145 150 155 160

Asn Ile Met Val His Ser Tyr Cys Lys Ser Leu Glu Phe Asp Lys Ala

165 170 175

Asp Thr Val Ile Ser Glu Met Glu Lys Arg Cys Val Phe Pro Asp Val

180 185 190

Val Thr His Asn Val Leu Ile Asp Ala Arg Phe Arg Ala Gly Asp Val

195 200 205

Asp Ala Ala Ile Ala Leu Val Asp Ser Met Ala Asn Lys Gly Leu Lys

210 215 220

Pro Gly Ile Val Thr Phe Asn Ser Val Leu Lys Gly Leu Cys Lys His

225 230 235 240

Arg Arg Phe Asp Lys Ala Lys Glu Val Phe Arg Ala Met Asp Gln Cys

245 250 255

Ser Val Ala Pro Asp Val Arg Ser Phe Asn Ile Leu Ile Gly Gly Phe

260 265 270

Cys Arg Val Gly Glu Val Glu Glu Ala Met Lys Phe Tyr Lys Glu Met

275 280 285

Gln Gln Arg Gly Val Thr Pro Asp Val Val Ser Phe Ser Cys Leu Ile

290 295 300

Gly Leu Phe Ser Thr Arg Gly Lys Met Asp His Ala Ala Ala Tyr Leu

305 310 315 320

Arg Glu Met Lys Gly Leu Gly Leu Val Pro Asp Gly Val Ile Tyr Thr

325 330 335

Met Val Ile Gly Gly Phe Cys Arg Ala Gly Ser Met Ser Glu Ala Leu

340 345 350

Arg Val Arg Asp Glu Met Val Gly Leu Gly Cys Leu Pro Asp Val Val

355 360 365

Thr Tyr Asn Thr Leu Leu Asn Gly Leu Cys Lys Gln His Arg Leu Leu

370 375 380

Asp Ala Glu Glu Leu Leu Asn Glu Met Lys Glu Arg Gly Val Thr Pro

385 390 395 400

Asp Leu Cys Thr Phe Thr Thr Leu Ile His Gly Tyr Cys Arg Asp Gly

405 410 415

Asn Phe Glu Lys Ala Leu Gln Leu Phe Asp Thr Leu Leu His Gln Arg

420 425 430

Leu Arg Pro Asp Val Val Ala Tyr Asn Ser Leu Ile Asp Gly Met Cys

435 440 445

Arg Lys Gly Asp Leu Ala Lys Ala Asn Glu Leu Trp Asp Asp Met His

450 455 460

Ala Arg Glu Ile Phe Pro Asn His Val Thr Tyr Ser Ile Leu Ile Asp

465 470 475 480

Ser His Cys Glu Lys Gly Gln Val Glu Glu Ala Phe Gly Phe Leu Asp

485 490 495

Glu Met Val Ser Lys Gly Asn Leu Pro Asn Ile Met Thr Tyr Asn Ser

500 505 510

Ile Ile Lys Gly Tyr Cys Arg Ser Gly Asn Val Lys Lys Gly Gln Gln

515 520 525

Phe Leu Gln Lys Met Met Gln Asp Asn Ile Leu Pro Asp Leu Ile Thr

530 535 540

Phe Asn Thr Leu Ile His Gly Tyr Ile Lys Glu Glu Asn Met His Gly

545 550 555 560

Ala Phe Asn Val Phe Asn Ile Met Glu Lys Glu Met Val Gln Pro Asp

565 570 575

Ala Val Thr Tyr Asn Met Ile Ile Asn Gly Phe Ser Glu Gln Gly Asn

580 585 590

Met Glu Glu Ala Gly Arg Val Phe Lys Lys Met Gly Ala Ser Gly Ile

595 600 605

Glu Pro Asp Arg Tyr Thr Tyr Met Ser Leu Ile Asn Gly His Val Thr

610 615 620

Ala Gly Asn Ser Lys Glu Ala Phe Gln Leu His Asp Glu Met Met His

625 630 635 640

Arg Gly Phe Ala Pro Asp Asp Lys Phe

645

<210> 89

<211> 2190

<212> DNA

<213> Arabidopsis thaliana

<400> 89

atgattgttc accgtataat accttcccgc gtaaaagacc ctctcactcg atttaaacct 60

ctcaaaaact tgacaacttc ttcatctccc gtattcgaac cctcttcttc ttcttcttct 120

tcttcttctt cggcttcgtt ttctgtttca gattctttct tggtggagaa gatttgtttt 180

agtttgaagc aaggtaacaa taatgtgaga aaccacctga ttcggttaaa ccctttagcc 240

gtcgttgagg ttttataccg ctgccgcaat gatttaactt taggtcaaag attcgtggat 300

caattaggtt tccattttcc gaatttcaag catacgtctt tgtctttaag cgctatgatt 360

catattctcg tgaggagtgg aagattatca gatgcacaga gttgtcttct taggatgatt 420

aggaggagtg gtgtttctag actagagatt gtgaattcct tggattctac gtttagtaat 480

tgtggttcca atgattcggt ttttgatttg ttgattagga cttatgttca ggctaggaaa 540

cttagagaag cacatgaggc ttttacattg ctgagaagca aaggttttac ggtttctatc 600

gatgcgtgta acgctcttat cgggagcttg gtgaggatcg ggtgggttga actggcttgg 660

ggtgtttatc aggagatttc tcggagtggt gttggtatta atgtctatac tctcaatatc 720

atggttaatg ctttgtgcaa agatggtaaa atggaaaaag tcggaacctt tttatcacaa 780

gtgcaagaga aaggggttta tccggatatt gtaacatata atacattgat tagtgcctac 840

tctagtaaag gtctcatgga agaggcattt gagttgatga atgctatgcc gggcaaaggg 900

tttagtcctg gtgtttatac ttataatact gtgataaatg gactgtgtaa gcatgggaaa 960

tatgagagag ccaaggaagt tttcgcggag atgttaagat ccgggttgag tcctgactcc 1020

actacctaca gatcgttgct gatggaggct tgtaagaagg gagatgtggt tgaaacggaa 1080

aaggttttca gtgatatgag atctagagat gttgttcctg atttggtttg ctttagctcg 1140

atgatgagtc tttttactcg gagtggaaat ctcgataagg cattaatgta ttttaacagt 1200

gtgaaggagg ctggtctgat tcctgacaat gtgatctata ccattcttat ccaagggtat 1260

tgtagaaaag gtatgatctc tgtagctatg aatttgagaa atgaaatgct tcagcagggt 1320

tgtgctatgg atgttgttac ctacaacacc attttgcatg gcttatgcaa acggaaaatg 1380

ttgggtgaag cggataaatt gttcaacgaa atgactgaaa gggctttgtt tccagattct 1440

tatactttaa ctatactgat tgatggacat tgtaagctcg ggaatctgca gaacgcaatg 1500

gagctctttc aaaagatgaa ggaaaagaga atcagactcg atgttgtgac atacaacact 1560

ctgttagatg ggtttggtaa agtaggcgat atcgatacag caaaagagat atgggcagat 1620

atggtatcaa aagagatact tcccactcca atatcttaca gcattttggt taacgcgttg 1680

tgcagtaaag gccatctggc tgaggcattt cgagtttggg atgaaatgat cagtaaaaat 1740

ataaagccaa cggtaatgat ttgtaactct atgataaagg gatattgtcg ttctggtaat 1800

gcctcagatg gagaaagctt tttagagaag atgatctcgg aaggttttgt gccggattgc 1860

atcagttaca acacattaat atatggtttt gttagagaag aaaacatgag caaagctttt 1920

ggtttggtaa agaagatgga ggaggagcaa ggaggattag tacctgatgt atttacatat 1980

aattcgatct tacatgggtt ttgtagacaa aatcagatga aagaagccga ggttgtgcta 2040

aggaagatga tcgagagagg tgtcaatccc gataggtcaa cgtatacttg tatgatcaat 2100

ggatttgttt ctcaagataa cttaaccgag gcatttcgga ttcacgatga gatgctgcaa 2160

agaggattct ctcctgatga taaattctga 2190

<210> 90

<211> 729

<212> PRT

<213> Arabidopsis thaliana

<400> 90

Met Ile Val His Arg Ile Ile Pro Ser Arg Val Lys Asp Pro Leu Thr

1 5 10 15

Arg Phe Lys Pro Leu Lys Asn Leu Thr Thr Ser Ser Ser Pro Val Phe

20 25 30

Glu Pro Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ala Ser Phe Ser

35 40 45

Val Ser Asp Ser Phe Leu Val Glu Lys Ile Cys Phe Ser Leu Lys Gln

50 55 60

Gly Asn Asn Asn Val Arg Asn His Leu Ile Arg Leu Asn Pro Leu Ala

65 70 75 80

Val Val Glu Val Leu Tyr Arg Cys Arg Asn Asp Leu Thr Leu Gly Gln

85 90 95

Arg Phe Val Asp Gln Leu Gly Phe His Phe Pro Asn Phe Lys His Thr

100 105 110

Ser Leu Ser Leu Ser Ala Met Ile His Ile Leu Val Arg Ser Gly Arg

115 120 125

Leu Ser Asp Ala Gln Ser Cys Leu Leu Arg Met Ile Arg Arg Ser Gly

130 135 140

Val Ser Arg Leu Glu Ile Val Asn Ser Leu Asp Ser Thr Phe Ser Asn

145 150 155 160

Cys Gly Ser Asn Asp Ser Val Phe Asp Leu Leu Ile Arg Thr Tyr Val

165 170 175

Gln Ala Arg Lys Leu Arg Glu Ala His Glu Ala Phe Thr Leu Leu Arg

180 185 190

Ser Lys Gly Phe Thr Val Ser Ile Asp Ala Cys Asn Ala Leu Ile Gly

195 200 205

Ser Leu Val Arg Ile Gly Trp Val Glu Leu Ala Trp Gly Val Tyr Gln

210 215 220

Glu Ile Ser Arg Ser Gly Val Gly Ile Asn Val Tyr Thr Leu Asn Ile

225 230 235 240

Met Val Asn Ala Leu Cys Lys Asp Gly Lys Met Glu Lys Val Gly Thr

245 250 255

Phe Leu Ser Gln Val Gln Glu Lys Gly Val Tyr Pro Asp Ile Val Thr

260 265 270

Tyr Asn Thr Leu Ile Ser Ala Tyr Ser Ser Lys Gly Leu Met Glu Glu

275 280 285

Ala Phe Glu Leu Met Asn Ala Met Pro Gly Lys Gly Phe Ser Pro Gly

290 295 300

Val Tyr Thr Tyr Asn Thr Val Ile Asn Gly Leu Cys Lys His Gly Lys

305 310 315 320

Tyr Glu Arg Ala Lys Glu Val Phe Ala Glu Met Leu Arg Ser Gly Leu

325 330 335

Ser Pro Asp Ser Thr Thr Tyr Arg Ser Leu Leu Met Glu Ala Cys Lys

340 345 350

Lys Gly Asp Val Val Glu Thr Glu Lys Val Phe Ser Asp Met Arg Ser

355 360 365

Arg Asp Val Val Pro Asp Leu Val Cys Phe Ser Ser Met Met Ser Leu

370 375 380

Phe Thr Arg Ser Gly Asn Leu Asp Lys Ala Leu Met Tyr Phe Asn Ser

385 390 395 400

Val Lys Glu Ala Gly Leu Ile Pro Asp Asn Val Ile Tyr Thr Ile Leu

405 410 415

Ile Gln Gly Tyr Cys Arg Lys Gly Met Ile Ser Val Ala Met Asn Leu

420 425 430

Arg Asn Glu Met Leu Gln Gln Gly Cys Ala Met Asp Val Val Thr Tyr

435 440 445

Asn Thr Ile Leu His Gly Leu Cys Lys Arg Lys Met Leu Gly Glu Ala

450 455 460

Asp Lys Leu Phe Asn Glu Met Thr Glu Arg Ala Leu Phe Pro Asp Ser

465 470 475 480

Tyr Thr Leu Thr Ile Leu Ile Asp Gly His Cys Lys Leu Gly Asn Leu

485 490 495

Gln Asn Ala Met Glu Leu Phe Gln Lys Met Lys Glu Lys Arg Ile Arg

500 505 510

Leu Asp Val Val Thr Tyr Asn Thr Leu Leu Asp Gly Phe Gly Lys Val

515 520 525

Gly Asp Ile Asp Thr Ala Lys Glu Ile Trp Ala Asp Met Val Ser Lys

530 535 540

Glu Ile Leu Pro Thr Pro Ile Ser Tyr Ser Ile Leu Val Asn Ala Leu

545 550 555 560

Cys Ser Lys Gly His Leu Ala Glu Ala Phe Arg Val Trp Asp Glu Met

565 570 575

Ile Ser Lys Asn Ile Lys Pro Thr Val Met Ile Cys Asn Ser Met Ile

580 585 590

Lys Gly Tyr Cys Arg Ser Gly Asn Ala Ser Asp Gly Glu Ser Phe Leu

595 600 605

Glu Lys Met Ile Ser Glu Gly Phe Val Pro Asp Cys Ile Ser Tyr Asn

610 615 620

Thr Leu Ile Tyr Gly Phe Val Arg Glu Glu Asn Met Ser Lys Ala Phe

625 630 635 640

Gly Leu Val Lys Lys Met Glu Glu Glu Gln Gly Gly Leu Val Pro Asp

645 650 655

Val Phe Thr Tyr Asn Ser Ile Leu His Gly Phe Cys Arg Gln Asn Gln

660 665 670

Met Lys Glu Ala Glu Val Val Leu Arg Lys Met Ile Glu Arg Gly Val

675 680 685

Asn Pro Asp Arg Ser Thr Tyr Thr Cys Met Ile Asn Gly Phe Val Ser

690 695 700

Gln Asp Asn Leu Thr Glu Ala Phe Arg Ile His Asp Glu Met Leu Gln

705 710 715 720

Arg Gly Phe Ser Pro Asp Asp Lys Phe

725

<210> 91

<211> 1779

<212> DNA

<213> Glycine max

<400> 91

gtccacgacc tcgttcgtgc ccggaacctc ccagaagccc aatccctcct cctccgaatg 60

atccgaaaac gcggcgtttc gcgcccccaa ctcatcgatt ccctccttcc ttcttctccc 120

agttccactc acaccaacgc caccgttctc gatttgctca tcagaaccta cgttcagtcg 180

cggaagcttc gagaaggctc ggaagccttc cgcttgctga ggcaaaaggg cttctccgtt 240

tccatcaacg cctccaacgc gcttctgggc gcgttggtca aagtggggtg ggtcgacttg 300

gcatggacag tgtacgagga tgttgtcgca agtggcacaa cggtaaatgt ctacactctc 360

aacatcatgg tcaatgcttt gtgtaaagag gccaggttcg acaaggttaa ggttttcttg 420

tcccaaatgg agggaaaagg tgttttccct gatgttgtaa cttataacac tttaatcaac 480

gcgcattctc gtcagggtaa tgtggcagag gcttttgagc tcctgggctt ttatacttat 540

aatgctattg ttaatgggtt gtgtaagaag ggggattatg tgagagcgag gggtgttttt 600

gatgagatgt tggggatggg gttgagtcct gatgctgcca cttttaatcc cttgcttgtg 660

gaatgttgta ggaaggatga tgcttgtgag gctgagaatg tttttgatga aatgttgcgg 720

tacggggttg ttcctgattt gattagtttt ggatctgtga ttggggtgtt ctccaggaac 780

gggctcttcg ataaggcgtt ggagtatttt gggaagatga agggttctgg attggttgct 840

gatacggtga tttataccat tcttattgac gggtattgca ggaatggcaa tgtggctgag 900

gcattggcca tgcggaacga gatggttgaa aagggttgct ttatggatgt ggttacgtat 960

aatactctgc tgaatgggtt gtgtaggggg aagatgcttg gtgatgccga tgagttgttt 1020

aaggagatgg tggagagggg agtttttccg gattactata cccttaccac tctcattcat 1080

gggtattgta aggatgggaa catgagtagg gcacttggtt tgtttgagac gatgactcaa 1140

aggagcctta agccggatgt tgtgacatat aatacattga tggatggctt ttgcaagatt 1200

ggtgaaatgg agaaagctaa ggagttgtgg cgtgatatgg tgagcagggg gatattgccc 1260

aactatgtat cgtttagcat tttgataaat gggttttgca gtttaggact tatgggtgag 1320

gcattcaggg tgtgggatga aatgatagaa aaaggtgtta agcccacttt ggttacttgc 1380

aacactgtta taaagggcca tttgcgggct ggcaatgtgt taaaggcaaa tgacttcttc 1440

gaaaagatga ttttggaagg agtttctcct gattgcatca cgtataatac tcttataaat 1500

ggttttgtaa aggaagagaa tttcgacagg gcttttgtct tggttaataa catggaagaa 1560

aaagggctac tgccagatgt catcacttat aatgcaattc tcggtggata ttgtaggcaa 1620

ggcagaatgc gggaggctga gatggtgtta cgcaagatga ttgactgtgg catcaatcct 1680

gataaatcaa catacacatc attgataaat gggcatgtat ctctagacaa cttgaaagaa 1740

gcattccgtt tccacgatga aatgctgcaa aggggattt 1779

<210> 92

<211> 593

<212> PRT

<213> Glycine max

<400> 92

Val His Asp Leu Val Arg Ala Arg Asn Leu Pro Glu Ala Gln Ser Leu

1 5 10 15

Leu Leu Arg Met Ile Arg Lys Arg Gly Val Ser Arg Pro Gln Leu Ile

20 25 30

Asp Ser Leu Leu Pro Ser Ser Pro Ser Ser Thr His Thr Asn Ala Thr

35 40 45

Val Leu Asp Leu Leu Ile Arg Thr Tyr Val Gln Ser Arg Lys Leu Arg

50 55 60

Glu Gly Ser Glu Ala Phe Arg Leu Leu Arg Gln Lys Gly Phe Ser Val

65 70 75 80

Ser Ile Asn Ala Ser Asn Ala Leu Leu Gly Ala Leu Val Lys Val Gly

85 90 95

Trp Val Asp Leu Ala Trp Thr Val Tyr Glu Asp Val Val Ala Ser Gly

100 105 110

Thr Thr Val Asn Val Tyr Thr Leu Asn Ile Met Val Asn Ala Leu Cys

115 120 125

Lys Glu Ala Arg Phe Asp Lys Val Lys Val Phe Leu Ser Gln Met Glu

130 135 140

Gly Lys Gly Val Phe Pro Asp Val Val Thr Tyr Asn Thr Leu Ile Asn

145 150 155 160

Ala His Ser Arg Gln Gly Asn Val Ala Glu Ala Phe Glu Leu Leu Gly

165 170 175

Phe Tyr Thr Tyr Asn Ala Ile Val Asn Gly Leu Cys Lys Lys Gly Asp

180 185 190

Tyr Val Arg Ala Arg Gly Val Phe Asp Glu Met Leu Gly Met Gly Leu

195 200 205

Ser Pro Asp Ala Ala Thr Phe Asn Pro Leu Leu Val Glu Cys Cys Arg

210 215 220

Lys Asp Asp Ala Cys Glu Ala Glu Asn Val Phe Asp Glu Met Leu Arg

225 230 235 240

Tyr Gly Val Val Pro Asp Leu Ile Ser Phe Gly Ser Val Ile Gly Val

245 250 255

Phe Ser Arg Asn Gly Leu Phe Asp Lys Ala Leu Glu Tyr Phe Gly Lys

260 265 270

Met Lys Gly Ser Gly Leu Val Ala Asp Thr Val Ile Tyr Thr Ile Leu

275 280 285

Ile Asp Gly Tyr Cys Arg Asn Gly Asn Val Ala Glu Ala Leu Ala Met

290 295 300

Arg Asn Glu Met Val Glu Lys Gly Cys Phe Met Asp Val Val Thr Tyr

305 310 315 320

Asn Thr Leu Leu Asn Gly Leu Cys Arg Gly Lys Met Leu Gly Asp Ala

325 330 335

Asp Glu Leu Phe Lys Glu Met Val Glu Arg Gly Val Phe Pro Asp Tyr

340 345 350

Tyr Thr Leu Thr Thr Leu Ile His Gly Tyr Cys Lys Asp Gly Asn Met

355 360 365

Ser Arg Ala Leu Gly Leu Phe Glu Thr Met Thr Gln Arg Ser Leu Lys

370 375 380

Pro Asp Val Val Thr Tyr Asn Thr Leu Met Asp Gly Phe Cys Lys Ile

385 390 395 400

Gly Glu Met Glu Lys Ala Lys Glu Leu Trp Arg Asp Met Val Ser Arg

405 410 415

Gly Ile Leu Pro Asn Tyr Val Ser Phe Ser Ile Leu Ile Asn Gly Phe

420 425 430

Cys Ser Leu Gly Leu Met Gly Glu Ala Phe Arg Val Trp Asp Glu Met

435 440 445

Ile Glu Lys Gly Val Lys Pro Thr Leu Val Thr Cys Asn Thr Val Ile

450 455 460

Lys Gly His Leu Arg Ala Gly Asn Val Leu Lys Ala Asn Asp Phe Phe

465 470 475 480

Glu Lys Met Ile Leu Glu Gly Val Ser Pro Asp Cys Ile Thr Tyr Asn

485 490 495

Thr Leu Ile Asn Gly Phe Val Lys Glu Glu Asn Phe Asp Arg Ala Phe

500 505 510

Val Leu Val Asn Asn Met Glu Glu Lys Gly Leu Leu Pro Asp Val Ile

515 520 525

Thr Tyr Asn Ala Ile Leu Gly Gly Tyr Cys Arg Gln Gly Arg Met Arg

530 535 540

Glu Ala Glu Met Val Leu Arg Lys Met Ile Asp Cys Gly Ile Asn Pro

545 550 555 560

Asp Lys Ser Thr Tyr Thr Ser Leu Ile Asn Gly His Val Ser Leu Asp

565 570 575

Asn Leu Lys Glu Ala Phe Arg Phe His Asp Glu Met Leu Gln Arg Gly

580 585 590

Phe

<210> 93

<211> 726

<212> DNA

<213> Zea mays

<400> 93

atgcatgcat gcatgtggct gcttgcttgc ttgcttgcat tctgtacaag aagaacttca 60

tcgatcgatc gatcgatcct cgagtccagg actgaaccga accgatctga tctgatctac 120

gcgtacgcac cacgccaaca agcagatcgt tacagacaat taagaaggaa cctagctagc 180

ttgcggcatc taccggcaga cggtggcttt cttcttcccc ttcccggcgg cgttcttgcg 240

ctggaggcgg cgcaggaggt cctccaggtc ctcctcggcg cacggcacgc gcaggccgcc 300

cgcctgcgcg aagccgaact cgtcggcggc gcgctccatg aggtcccgga acgccggctc 360

ccggaggtac ccggtgggca cgacgaaccg gcgccgcgac tcctccccgg cgtacaccgc 420

gaagtagccc ttgggcacgc cgccctcctc ctccgccgcc gccggctggt ccagcagcgc 480

ctcccggagg cccagctcct cgcgcgtcgt cctcgccttc tgcttggccg ccgccgtcgc 540

ggacttggtc gtcgacgagc ggaagtagcc catcaccatc atcgtgtcac tcgtgcgtcg 600

tcggtcggtc ggtcgtactc gtacgtatac gtagcgtggc cgccgggcga gtgcagccgt 660

gaacacgtac gtacgcaaca actaacgccg ggcgagtgcg cagagggctt agctagtagt 720

aggtaa 726

<210> 94

<211> 241

<212> PRT

<213> Zea mays

<400> 94

Met His Ala Cys Met Trp Leu Leu Ala Cys Leu Leu Ala Phe Cys Thr

1 5 10 15

Arg Arg Thr Ser Ser Ile Asp Arg Ser Ile Leu Glu Ser Arg Thr Glu

20 25 30

Pro Asn Arg Ser Asp Leu Ile Tyr Ala Tyr Ala Pro Arg Gln Gln Ala

35 40 45

Asp Arg Tyr Arg Gln Leu Arg Arg Asn Leu Ala Ser Leu Arg His Leu

50 55 60

Pro Ala Asp Gly Gly Phe Leu Leu Pro Leu Pro Gly Gly Val Leu Ala

65 70 75 80

Leu Glu Ala Ala Gln Glu Val Leu Gln Val Leu Leu Gly Ala Arg His

85 90 95

Ala Gln Ala Ala Arg Leu Arg Glu Ala Glu Leu Val Gly Gly Ala Leu

100 105 110

His Glu Val Pro Glu Arg Arg Leu Pro Glu Val Pro Gly Gly His Asp

115 120 125

Glu Pro Ala Pro Arg Leu Leu Pro Gly Val His Arg Glu Val Ala Leu

130 135 140

Gly His Ala Ala Leu Leu Leu Arg Arg Arg Arg Leu Val Gln Gln Arg

145 150 155 160

Leu Pro Glu Ala Gln Leu Leu Ala Arg Arg Pro Arg Leu Leu Leu Gly

165 170 175

Arg Arg Arg Arg Gly Leu Gly Arg Arg Arg Ala Glu Val Ala His His

180 185 190

His His Arg Val Thr Arg Ala Ser Ser Val Gly Arg Ser Tyr Ser Tyr

195 200 205

Val Tyr Val Ala Trp Pro Pro Gly Glu Cys Ser Arg Glu His Val Arg

210 215 220

Thr Gln Gln Leu Thr Pro Gly Glu Cys Ala Glu Gly Leu Ala Ser Ser

225 230 235 240

Arg

<210> 95

<211> 405

<212> DNA

<213> Sorghum bicolor

<400> 95

atgatggtga tgggctactt ccggtcgacg aacaagtccg cgacgtcggc agcgggcggc 60

aagaacaaga agaaggcgaa gaacagctgt gcgccggcgg cgcgcgagga ggcggccgga 120

ctgcgggagg cgctgctgga ccagccggcg gcggcggagg aggacggcgg cgttcctaag 180

ggctacttcg cggtgtacgc cggggaggag tcccgccggt tcgtcgtgcc cacggggtac 240

ctccgggagc cggcgttccg ggacctcatg gagcgcgccg ccgacgagtt cggcttcgcg 300

caggccggcg gcctgcgcgt gccctgcgcc gaggaggact tcgaggacct cctgcgccgt 360

ctccagcgca agaacggcgg cgccgtcggg aagggcaaga aataa 405

<210> 96

<211> 134

<212> PRT

<213> Sorghum bicolor

<400> 96

Met Met Val Met Gly Tyr Phe Arg Ser Thr Asn Lys Ser Ala Thr Ser

1 5 10 15

Ala Ala Gly Gly Lys Asn Lys Lys Lys Ala Lys Asn Ser Cys Ala Pro

20 25 30

Ala Ala Arg Glu Glu Ala Ala Gly Leu Arg Glu Ala Leu Leu Asp Gln

35 40 45

Pro Ala Ala Ala Glu Glu Asp Gly Gly Val Pro Lys Gly Tyr Phe Ala

50 55 60

Val Tyr Ala Gly Glu Glu Ser Arg Arg Phe Val Val Pro Thr Gly Tyr

65 70 75 80

Leu Arg Glu Pro Ala Phe Arg Asp Leu Met Glu Arg Ala Ala Asp Glu

85 90 95

Phe Gly Phe Ala Gln Ala Gly Gly Leu Arg Val Pro Cys Ala Glu Glu

100 105 110

Asp Phe Glu Asp Leu Leu Arg Arg Leu Gln Arg Lys Asn Gly Gly Ala

115 120 125

Val Gly Lys Gly Lys Lys

130

<210> 97

<211> 537

<212> DNA

<213> Arabidopsis thaliana

<400> 97

atggaagcca agaagtcaaa caagatcaga gagatcgtta agcttcaaca gcttctcaag 60

aaatggcgaa aacaagcaat tgcatcaaaa gcagccaaca acaacaacga agacaacaat 120

agcagtggcg ggggaagcaa gagcatcaag tttctgaaga ggacactgtc atttacagat 180

gtaacagctg tgcctaaagg gtatctagct gtttcggtcg ggttagagaa gaagaggtac 240

acaataccaa ccgagtacct cagccatcaa gctttctatg ttcttctgcg tgaggcagaa 300

gaagagttcg ggtttcaaca agccggtgtc ttgaggattc cttgtgaagt ttctgttttt 360

gagagcatat tgaagataat ggaggagaag aacgaagggt acctggtgac gccaataaca 420

gctaagcagg aatgtaagtt caatgcagca gcagatgata agacgagtta ccagcatcca 480

tcagactgtc ctaagactcc ttcacaccaa cctcacaaca gcccaatgtg cagatag 537

<210> 98

<211> 178

<212> PRT

<213> Arabidopsis thaliana

<400> 98

Met Glu Ala Lys Lys Ser Asn Lys Ile Arg Glu Ile Val Lys Leu Gln

1 5 10 15

Gln Leu Leu Lys Lys Trp Arg Lys Gln Ala Ile Ala Ser Lys Ala Ala

20 25 30

Asn Asn Asn Asn Glu Asp Asn Asn Ser Ser Gly Gly Gly Ser Lys Ser

35 40 45

Ile Lys Phe Leu Lys Arg Thr Leu Ser Phe Thr Asp Val Thr Ala Val

50 55 60

Pro Lys Gly Tyr Leu Ala Val Ser Val Gly Leu Glu Lys Lys Arg Tyr

65 70 75 80

Thr Ile Pro Thr Glu Tyr Leu Ser His Gln Ala Phe Tyr Val Leu Leu

85 90 95

Arg Glu Ala Glu Glu Glu Phe Gly Phe Gln Gln Ala Gly Val Leu Arg

100 105 110

Ile Pro Cys Glu Val Ser Val Phe Glu Ser Ile Leu Lys Ile Met Glu

115 120 125

Glu Lys Asn Glu Gly Tyr Leu Val Thr Pro Ile Thr Ala Lys Gln Glu

130 135 140

Cys Lys Phe Asn Ala Ala Ala Asp Asp Lys Thr Ser Tyr Gln His Pro

145 150 155 160

Ser Asp Cys Pro Lys Thr Pro Ser His Gln Pro His Asn Ser Pro Met

165 170 175

Cys Arg

<210> 99

<211> 504

<212> DNA

<213> Glycine max

<400> 99

atggatagcg atggaggctc caaattgaat gggattaggc agattgtgaa gctaaaggaa 60

atgctccaga agtggcaaag tgtgacatta ggcaccaaac cttccaattc cctttctgat 120

catgtgacta atgatgatgg gagcatatca ccattgatca acaaaagtgt attgaatgtg 180

atgaattgcg aatcagacaa tgaggacagt tgccaaagcc ctgcagaacc gcttccacca 240

cctgatgttc cgaaagggta cttggcagtg tatgttggac ctgagcttag gaggttcatc 300

atccccacca gttaccttag ccaccctctc ttcaaagttt tgctggaaaa ggctgcagac 360

gaattcgggt ttgatcagag tggtggcctc accatcccat gtgagattga gaccttcaag 420

taccttctca agtgcatgga gaatgagcag aaagagcaac tcggtgacag cgcccctgga 480

agctcagggt ctgtcgaaga gtaa 504

<210> 100

<211> 167

<212> PRT

<213> Glycine max

<400> 100

Met Asp Ser Asp Gly Gly Ser Lys Leu Asn Gly Ile Arg Gln Ile Val

1 5 10 15

Lys Leu Lys Glu Met Leu Gln Lys Trp Gln Ser Val Thr Leu Gly Thr

20 25 30

Lys Pro Ser Asn Ser Leu Ser Asp His Val Thr Asn Asp Asp Gly Ser

35 40 45

Ile Ser Pro Leu Ile Asn Lys Ser Val Leu Asn Val Met Asn Cys Glu

50 55 60

Ser Asp Asn Glu Asp Ser Cys Gln Ser Pro Ala Glu Pro Leu Pro Pro

65 70 75 80

Pro Asp Val Pro Lys Gly Tyr Leu Ala Val Tyr Val Gly Pro Glu Leu

85 90 95

Arg Arg Phe Ile Ile Pro Thr Ser Tyr Leu Ser His Pro Leu Phe Lys

100 105 110

Val Leu Leu Glu Lys Ala Ala Asp Glu Phe Gly Phe Asp Gln Ser Gly

115 120 125

Gly Leu Thr Ile Pro Cys Glu Ile Glu Thr Phe Lys Tyr Leu Leu Lys

130 135 140

Cys Met Glu Asn Glu Gln Lys Glu Gln Leu Gly Asp Ser Ala Pro Gly

145 150 155 160

Ser Ser Gly Ser Val Glu Glu

165

<210> 101

<211> 1638

<212> DNA

<213> Oryza sativa

<400> 101

atgagaagcc caccggagag cgggtcgcgc accggtcgag gcgaagaaat tgcggcgcgg 60

caactaaacc ctaccgcctc tgcctcgccg ccacgctccc ttattatgga cggggcctgc 120

tgcgacggcg gtggctcgcc ggagtccggc ggggcgtcct cgtcggcgtc gtcgtacggc 180

tccgcgtccc ggctgcagaa gggggtgcgc ctgcggcggc ggcggcagag gctccggaga 240

ccgctgcttg cgactggagg ggatgggagg ggcgccgccg acggcgcgca ggacctcgcg 300

ctgcctctcg ggatgtcctt cgcggcggtt cttgcccagg tccttaatag aagcagttgc 360

tctgaaggaa gattacaacc tgatttcctt tcaaagatgt gcacgtcagc agtgaaagag 420

tccttgacaa atatatatgg tgatagattc gacaacttca cgaaaaactt tgagaaatca 480

tttggaagta cattgaggac acttcattta attaatgaga cacctgtcta tgagcaagat 540

aactctcggt tttctcacga agatggtact tctgctgctg aaatcaaatt gagtggtgct 600

gattcaaaaa ggccggtaca tgatatccag gagagtacat cgttaagttc aatggataat 660

caaatcattc ttcatgcggg caccgatcag cagttggtta aactacctca taataaagct 720

agtccagaat ttgataggca cattcttaat gtatttgaga gatctctgaa tgagcagact 780

cgttcaaatg agctcaagga acttgaaata ggactaaaca tgaggaaatt gcaattgaag 840

caatctcaga tagctctcag ctcatactca cacatgctag agaagatcaa aatttctatg 900

ggatttcaga aagcttcttt cagagaggaa aaatttagga cgcaaatgga ggacacaaga 960

catgctgaac tcctcaggag gcttatagat ttgctcctta ccgcagtggt atttatgtct 1020

gtctgttttg gttatggaac ctatatttat tcgtacaagc ggataactgc tgttactgca 1080

gcttgtgcag cggcttcaag ggaacctaaa tcttggtgga tgccaaattc agtatcagct 1140

ttcaattcgg gtttgctgtt tttcaggtgt catttaatag cagcaacaag gatgtcattt 1200

ggcatgttaa tgattctctt gattgcttgg ttgatattcc agcgttctgc aatgactgga 1260

ccaaacatgc caataacgtt caatgttatg ttattgggag ttctctgtgg ttctgtcgga 1320

aggttctgcg ttgacacact aggcggggat ggaaatgtct ggctcttttt ctgggagatt 1380

ctctgcttca tccatttatt tgggaacagc aggccatctc ttttataccg catgctttat 1440

ggtcctattt cagtgactga cagaacgaag gcgtctgatt taccatatcg ggtttgccga 1500

tacacatttt atactgtcct gtcggttatt ctcccatgct tggctggttt gctgccattt 1560

gcttccctgt cagactggaa tgagcttgtg gtagaatata tgaagtccaa attcattaga 1620

attaatactg aggtttga 1638

<210> 102

<211> 545

<212> PRT

<213> Oryza sativa

<400> 102

Met Arg Ser Pro Pro Glu Ser Gly Ser Arg Thr Gly Arg Gly Glu Glu

1 5 10 15

Ile Ala Ala Arg Gln Leu Asn Pro Thr Ala Ser Ala Ser Pro Pro Arg

20 25 30

Ser Leu Ile Met Asp Gly Ala Cys Cys Asp Gly Gly Gly Ser Pro Glu

35 40 45

Ser Gly Gly Ala Ser Ser Ser Ala Ser Ser Tyr Gly Ser Ala Ser Arg

50 55 60

Leu Gln Lys Gly Val Arg Leu Arg Arg Arg Arg Gln Arg Leu Arg Arg

65 70 75 80

Pro Leu Leu Ala Thr Gly Gly Asp Gly Arg Gly Ala Ala Asp Gly Ala

85 90 95

Gln Asp Leu Ala Leu Pro Leu Gly Met Ser Phe Ala Ala Val Leu Ala

100 105 110

Gln Val Leu Asn Arg Ser Ser Cys Ser Glu Gly Arg Leu Gln Pro Asp

115 120 125

Phe Leu Ser Lys Met Cys Thr Ser Ala Val Lys Glu Ser Leu Thr Asn

130 135 140

Ile Tyr Gly Asp Arg Phe Asp Asn Phe Thr Lys Asn Phe Glu Lys Ser

145 150 155 160

Phe Gly Ser Thr Leu Arg Thr Leu His Leu Ile Asn Glu Thr Pro Val

165 170 175

Tyr Glu Gln Asp Asn Ser Arg Phe Ser His Glu Asp Gly Thr Ser Ala

180 185 190

Ala Glu Ile Lys Leu Ser Gly Ala Asp Ser Lys Arg Pro Val His Asp

195 200 205

Ile Gln Glu Ser Thr Ser Leu Ser Ser Met Asp Asn Gln Ile Ile Leu

210 215 220

His Ala Gly Thr Asp Gln Gln Leu Val Lys Leu Pro His Asn Lys Ala

225 230 235 240

Ser Pro Glu Phe Asp Arg His Ile Leu Asn Val Phe Glu Arg Ser Leu

245 250 255

Asn Glu Gln Thr Arg Ser Asn Glu Leu Lys Glu Leu Glu Ile Gly Leu

260 265 270

Asn Met Arg Lys Leu Gln Leu Lys Gln Ser Gln Ile Ala Leu Ser Ser

275 280 285

Tyr Ser His Met Leu Glu Lys Ile Lys Ile Ser Met Gly Phe Gln Lys

290 295 300

Ala Ser Phe Arg Glu Glu Lys Phe Arg Thr Gln Met Glu Asp Thr Arg

305 310 315 320

His Ala Glu Leu Leu Arg Arg Leu Ile Asp Leu Leu Leu Thr Ala Val

325 330 335

Val Phe Met Ser Val Cys Phe Gly Tyr Gly Thr Tyr Ile Tyr Ser Tyr

340 345 350

Lys Arg Ile Thr Ala Val Thr Ala Ala Cys Ala Ala Ala Ser Arg Glu

355 360 365

Pro Lys Ser Trp Trp Met Pro Asn Ser Val Ser Ala Phe Asn Ser Gly

370 375 380

Leu Leu Phe Phe Arg Cys His Leu Ile Ala Ala Thr Arg Met Ser Phe

385 390 395 400

Gly Met Leu Met Ile Leu Leu Ile Ala Trp Leu Ile Phe Gln Arg Ser

405 410 415

Ala Met Thr Gly Pro Asn Met Pro Ile Thr Phe Asn Val Met Leu Leu

420 425 430

Gly Val Leu Cys Gly Ser Val Gly Arg Phe Cys Val Asp Thr Leu Gly

435 440 445

Gly Asp Gly Asn Val Trp Leu Phe Phe Trp Glu Ile Leu Cys Phe Ile

450 455 460

His Leu Phe Gly Asn Ser Arg Pro Ser Leu Leu Tyr Arg Met Leu Tyr

465 470 475 480

Gly Pro Ile Ser Val Thr Asp Arg Thr Lys Ala Ser Asp Leu Pro Tyr

485 490 495

Arg Val Cys Arg Tyr Thr Phe Tyr Thr Val Leu Ser Val Ile Leu Pro

500 505 510

Cys Leu Ala Gly Leu Leu Pro Phe Ala Ser Leu Ser Asp Trp Asn Glu

515 520 525

Leu Val Val Glu Tyr Met Lys Ser Lys Phe Ile Arg Ile Asn Thr Glu

530 535 540

Val

545

<210> 103

<211> 1575

<212> DNA

<213> Zea mays

<400> 103

atggacacgg gctgcctcca accaccctgc aacccctcct cgtcggaggc gtcgggaggc 60

gacgcctccg acgcctgcgg ctccgcctct gcctcccggt cctcctccgc ccgccgcagc 120

aagggggtgc ggctccggct ccggctgcgg cgaaggcggc aggagccggg ccccgccgct 180

acgcctgggg gtgagggtca gggtggcgcc ggcgtgcagg tgcaggacga cctcgcgctg 240

cccctcggga tgtcgttcgc ggccgtcctc gcgcaggttg tgaatacaaa gaatcgttca 300

ggcgaaagat tacagcctgc acttctttcc aagatctgta catcggcagt gaaggaatca 360

ttgagaaata tatatggtaa caagttagac agttttatga gaaactttga gaaatcattc 420

agcagcacat tgacaacgct tcatcttgtt aatgagatgc ctgtctatgg acaaggtcca 480

gttcctcaat gttcttctaa gcatgaagac tctgaagctt caagcaagtt gagcactggt 540

ggtacacaaa atctaacgcg tgaaatcaag caggagcgtt tgaattcagt ggaaagtcag 600

cttgttcttt atgctggcgg ctatcagcag atgactcgcc gcgctcatag cttatcttct 660

cctgaagctg atcagcgcat ccttaatgca tttgagagat ctttgaaaga acaaactcgg 720

tcaaatgagc tcaaggaatt tgagataagc cttagcatga gaaagttgaa gctaaaacag 780

tctcaactag aacttaactc ctattcacac atgttagaca agattaagtt atccttggga 840

tttcagaaag cttccttcca agtggaaaaa ttcaagactc agatgcagga cacgaggcat 900

gcacaaataa tgaggacact catagatttt cttgttagtg cagtgattat tatgtcagta 960

tgctttgggt atggaactta tacttattca taccaaagga taactgatat tacagcagca 1020

tgttcagcca cttcaaaggg atctaaatca tggtggatgc caaattcagt gtcaaacttc 1080

aatactgggt tgctcttcat aagatgtcat gtaatagctg caacacggat gttctttggt 1140

atagtaatga ttttggcagt tgtttggtta gcgttccagc gctctgcggt gtctgtatca 1200

agtatgccag taacttttaa catcattctg ttgggagtta tttgtggcta cgctggaagg 1260

ttctgtacca acacacttgg tggtgatggg aacatgtggc ttatatgctg ggaggtactt 1320

tgttccgtcc atttgattgg aaattgttat ccatcggtct tgtaccgtgt tctccatggt 1380

cctatatctg tatctcatag caaggagtct gtctggttcc cgtattggat tcgccgttgg 1440

atgttttatg ctgtgctggg gttttttatc ccagccttga atggcttact gccatttgct 1500

tctctttctg actggaataa ccatttcact aaggagctaa aatccatctt cattgatgag 1560

agaatcgaag cctga 1575

<210> 104

<211> 524

<212> PRT

<213> Zea mays

<400> 104

Met Asp Thr Gly Cys Leu Gln Pro Pro Cys Asn Pro Ser Ser Ser Glu

1 5 10 15

Ala Ser Gly Gly Asp Ala Ser Asp Ala Cys Gly Ser Ala Ser Ala Ser

20 25 30

Arg Ser Ser Ser Ala Arg Arg Ser Lys Gly Val Arg Leu Arg Leu Arg

35 40 45

Leu Arg Arg Arg Arg Gln Glu Pro Gly Pro Ala Ala Thr Pro Gly Gly

50 55 60

Glu Gly Gln Gly Gly Ala Gly Val Gln Val Gln Asp Asp Leu Ala Leu

65 70 75 80

Pro Leu Gly Met Ser Phe Ala Ala Val Leu Ala Gln Val Val Asn Thr

85 90 95

Lys Asn Arg Ser Gly Glu Arg Leu Gln Pro Ala Leu Leu Ser Lys Ile

100 105 110

Cys Thr Ser Ala Val Lys Glu Ser Leu Arg Asn Ile Tyr Gly Asn Lys

115 120 125

Leu Asp Ser Phe Met Arg Asn Phe Glu Lys Ser Phe Ser Ser Thr Leu

130 135 140

Thr Thr Leu His Leu Val Asn Glu Met Pro Val Tyr Gly Gln Gly Pro

145 150 155 160

Val Pro Gln Cys Ser Ser Lys His Glu Asp Ser Glu Ala Ser Ser Lys

165 170 175

Leu Ser Thr Gly Gly Thr Gln Asn Leu Thr Arg Glu Ile Lys Gln Glu

180 185 190

Arg Leu Asn Ser Val Glu Ser Gln Leu Val Leu Tyr Ala Gly Gly Tyr

195 200 205

Gln Gln Met Thr Arg Arg Ala His Ser Leu Ser Ser Pro Glu Ala Asp

210 215 220

Gln Arg Ile Leu Asn Ala Phe Glu Arg Ser Leu Lys Glu Gln Thr Arg

225 230 235 240

Ser Asn Glu Leu Lys Glu Phe Glu Ile Ser Leu Ser Met Arg Lys Leu

245 250 255

Lys Leu Lys Gln Ser Gln Leu Glu Leu Asn Ser Tyr Ser His Met Leu

260 265 270

Asp Lys Ile Lys Leu Ser Leu Gly Phe Gln Lys Ala Ser Phe Gln Val

275 280 285

Glu Lys Phe Lys Thr Gln Met Gln Asp Thr Arg His Ala Gln Ile Met

290 295 300

Arg Thr Leu Ile Asp Phe Leu Val Ser Ala Val Ile Ile Met Ser Val

305 310 315 320

Cys Phe Gly Tyr Gly Thr Tyr Thr Tyr Ser Tyr Gln Arg Ile Thr Asp

325 330 335

Ile Thr Ala Ala Cys Ser Ala Thr Ser Lys Gly Ser Lys Ser Trp Trp

340 345 350

Met Pro Asn Ser Val Ser Asn Phe Asn Thr Gly Leu Leu Phe Ile Arg

355 360 365

Cys His Val Ile Ala Ala Thr Arg Met Phe Phe Gly Ile Val Met Ile

370 375 380

Leu Ala Val Val Trp Leu Ala Phe Gln Arg Ser Ala Val Ser Val Ser

385 390 395 400

Ser Met Pro Val Thr Phe Asn Ile Ile Leu Leu Gly Val Ile Cys Gly

405 410 415

Tyr Ala Gly Arg Phe Cys Thr Asn Thr Leu Gly Gly Asp Gly Asn Met

420 425 430

Trp Leu Ile Cys Trp Glu Val Leu Cys Ser Val His Leu Ile Gly Asn

435 440 445

Cys Tyr Pro Ser Val Leu Tyr Arg Val Leu His Gly Pro Ile Ser Val

450 455 460

Ser His Ser Lys Glu Ser Val Trp Phe Pro Tyr Trp Ile Arg Arg Trp

465 470 475 480

Met Phe Tyr Ala Val Leu Gly Phe Phe Ile Pro Ala Leu Asn Gly Leu

485 490 495

Leu Pro Phe Ala Ser Leu Ser Asp Trp Asn Asn His Phe Thr Lys Glu

500 505 510

Leu Lys Ser Ile Phe Ile Asp Glu Arg Ile Glu Ala

515 520

<210> 105

<211> 1179

<212> DNA

<213> Sorghum bicolor

<400> 105

atgagaaact ttgagaaatc attcagcagc acattaacaa cgcttcatct tgttaatgag 60

atgcctgtct atggacaagg tccagttcct caatgttctt ctaagcatga agactctgat 120

gctgcaagca agttgagcac tgatggtcca caaaatccaa cgcaagaaat caagcaggac 180

ctttcaaatt cagtggaaag tcagcttgtt ctgtatgctg gtggcaatca gcagctgact 240

cgtcgtactc atagcatatc ttctcctgaa gctaatcggc ggatccttaa tgcatttgaa 300

agatctttga aagaacaaac tcggtcaaat gagctcaagg aatttgagat aagccttagc 360

atgagaaagt tgcaacttaa acagtctcaa ctagaactta actcctactc gcacatgtta 420

gacaagatta agttatcctt gggatttcag aaagcttcct tccaaacgga gaaattcaag 480

actcagatgc aggacacgag gcatgcacaa ataatgagga cactcataga tttccttgtt 540

agtgcagtta ttattatgtc agtatgtttt gggtacggaa cttatattta ttcataccaa 600

aggataactg atattacagc agcatgtgca gccacttcaa ggggatctaa atcatggtgg 660

gtgccaaatt cagtgtcaaa cttcaattcc gggttgctct tcatgagatg tcatgtagta 720

gctgcaacac ggatgttctt tggcatagta atgattctgg caattgtttg gttagcattc 780

cagcgttctg cagtgtctgg atcaagtatg ccagtaactt tcaacatcat actgttggga 840

attatttgtg gctttgctgg aaggttctgt accaacacac ttggtggcga tgggaacatg 900

tggcttatat gctgggaggt tctttgttcc atccatttac ttggaaattg ttatccatcg 960

gtcttgtacc gtgttcttca tggtcctata tctgtatctg acagcaagga gtctgtctgg 1020

ttcccgtatt ggattcgccg ctggatcttt tatgctgcgc tggggttttt tatcccagcc 1080

ttgaatggct tactgccatt tgcttctctt tctgactgga ataaccattt cactaaggag 1140

ctaaaatcca tctttattgg tgagaaaatt gaagcttga 1179

<210> 106

<211> 392

<212> PRT

<213> Sorghum bicolor

<400> 106

Met Arg Asn Phe Glu Lys Ser Phe Ser Ser Thr Leu Thr Thr Leu His

1 5 10 15

Leu Val Asn Glu Met Pro Val Tyr Gly Gln Gly Pro Val Pro Gln Cys

20 25 30

Ser Ser Lys His Glu Asp Ser Asp Ala Ala Ser Lys Leu Ser Thr Asp

35 40 45

Gly Pro Gln Asn Pro Thr Gln Glu Ile Lys Gln Asp Leu Ser Asn Ser

50 55 60

Val Glu Ser Gln Leu Val Leu Tyr Ala Gly Gly Asn Gln Gln Leu Thr

65 70 75 80

Arg Arg Thr His Ser Ile Ser Ser Pro Glu Ala Asn Arg Arg Ile Leu

85 90 95

Asn Ala Phe Glu Arg Ser Leu Lys Glu Gln Thr Arg Ser Asn Glu Leu

100 105 110

Lys Glu Phe Glu Ile Ser Leu Ser Met Arg Lys Leu Gln Leu Lys Gln

115 120 125

Ser Gln Leu Glu Leu Asn Ser Tyr Ser His Met Leu Asp Lys Ile Lys

130 135 140

Leu Ser Leu Gly Phe Gln Lys Ala Ser Phe Gln Thr Glu Lys Phe Lys

145 150 155 160

Thr Gln Met Gln Asp Thr Arg His Ala Gln Ile Met Arg Thr Leu Ile

165 170 175

Asp Phe Leu Val Ser Ala Val Ile Ile Met Ser Val Cys Phe Gly Tyr

180 185 190

Gly Thr Tyr Ile Tyr Ser Tyr Gln Arg Ile Thr Asp Ile Thr Ala Ala

195 200 205

Cys Ala Ala Thr Ser Arg Gly Ser Lys Ser Trp Trp Val Pro Asn Ser

210 215 220

Val Ser Asn Phe Asn Ser Gly Leu Leu Phe Met Arg Cys His Val Val

225 230 235 240

Ala Ala Thr Arg Met Phe Phe Gly Ile Val Met Ile Leu Ala Ile Val

245 250 255

Trp Leu Ala Phe Gln Arg Ser Ala Val Ser Gly Ser Ser Met Pro Val

260 265 270

Thr Phe Asn Ile Ile Leu Leu Gly Ile Ile Cys Gly Phe Ala Gly Arg

275 280 285

Phe Cys Thr Asn Thr Leu Gly Gly Asp Gly Asn Met Trp Leu Ile Cys

290 295 300

Trp Glu Val Leu Cys Ser Ile His Leu Leu Gly Asn Cys Tyr Pro Ser

305 310 315 320

Val Leu Tyr Arg Val Leu His Gly Pro Ile Ser Val Ser Asp Ser Lys

325 330 335

Glu Ser Val Trp Phe Pro Tyr Trp Ile Arg Arg Trp Ile Phe Tyr Ala

340 345 350

Ala Leu Gly Phe Phe Ile Pro Ala Leu Asn Gly Leu Leu Pro Phe Ala

355 360 365

Ser Leu Ser Asp Trp Asn Asn His Phe Thr Lys Glu Leu Lys Ser Ile

370 375 380

Phe Ile Gly Glu Lys Ile Glu Ala

385 390

<210> 107

<211> 1695

<212> DNA

<213> Arabidopsis thaliana

<400> 107

atggaagccc tcctcctccc tccttcgccg gaaccccaaa atcaaatcac caatccggcg 60

aattcaaagc caaatcatca atctggtgac gtacataaag atgagacgat gatgatgaag 120

aagaagaagg atacgaatcc atcgaatttg gaaaagagaa aactcaaggg aaagaagaaa 180

gagattatgg acaacgacga agcttcttcg tcctattgtt ctacatcttc tacctctaat 240

tcaaattcta ctaaaagggt tacgagagtg gttcatagat tacgaaaccc tatgcggtta 300

ggtatggctc gacgaagcgt tggtgaacga caagctgaaa aattggcgaa gcctctgggc 360

ttttcacttg ccgcttttgc taatatggtt attgcgagaa agaatgccgc aggtcagaat 420

gtttatgttg atgatcttgt tgagatcttt gctactcttg tcgaagaatc attagccaat 480

gtttatggta ataagcttgg ttcctttgcg accaactttg agcaaacatt cagcagtact 540

ctaaagatcc ttaaattgac caatgaatgt gcaaatccac atcagtcaaa caataatgat 600

ggtgggagtt gtaatttaga tcgctctacc atagacggat gctcagacac cgagctattt 660

gagagggaga cttcatctgc tacgtctgct tatgaagtga tgcaaggcag tgcaacagca 720

acctctttga tgaatgagct tgcccttttc gaagagactc tacaactctc ttgtgtccct 780

cctagaagtt cagcaatggc tttgaccaca gacgaaaggt ttttaaaaga gcaaacacga 840

gcaaacgacc taaagaccgt ggagattggt cttcaaataa gagagttaag gtgcaaagag 900

acggcgctag gattaaaatt tgaatcaaac aacctgggga aagcggcgct agagttggat 960

gtttcgaaag ctgcattcag agcggagaaa ttcaaaaccg aattagaaga tacaagaaaa 1020

gaagagatgg tcacaagaat catggattgg ctcctcgtaa gtgtcttcag catgttggct 1080

tctatggtac ttggcgttta caatttttca ataaagagaa tcgaggatgc tacctcagta 1140

tgcgaccaat ccgaggagaa aagttcgtcg tggtgggttc ctaaacaagt ttcatcgatt 1200

aactcaggct tcaacacctt catctgccgg gttcgagttt gggtgcagat atttttcggt 1260

gtgttaatga tcattgtctt cacttacttt ctaaacaaac gatcatcagg tacgaagcag 1320

acaatgccga taagtttcat cgttcttttc ctcggtatat tttgcggtgt atcgggtaaa 1380

ttgtgtgtgg acacattggg cggtgatggc aaactctggc taatagtttg ggaagtgttt 1440

tgccttttgc aattcgttgc aaatgtcttc acattggctt tgtatggtct aatgttcggt 1500

cctataaacg tgactcaaga gaccagatcg aaccgttgta acagtatgtt tccatattgg 1560

gcaaggcgca gtgtcgtgta tgtggtgatt ctgtttgttc ttccagtcat aaacggtctt 1620

ttgccatttg caacatttgg tgaatggaga gacttcgcta tgtatcacct tcatggtggg 1680

tctgactatg cttga 1695

<210> 108

<211> 564

<212> PRT

<213> Arabidopsis thaliana

<400> 108

Met Glu Ala Leu Leu Leu Pro Pro Ser Pro Glu Pro Gln Asn Gln Ile

1 5 10 15

Thr Asn Pro Ala Asn Ser Lys Pro Asn His Gln Ser Gly Asp Val His

20 25 30

Lys Asp Glu Thr Met Met Met Lys Lys Lys Lys Asp Thr Asn Pro Ser

35 40 45

Asn Leu Glu Lys Arg Lys Leu Lys Gly Lys Lys Lys Glu Ile Met Asp

50 55 60

Asn Asp Glu Ala Ser Ser Ser Tyr Cys Ser Thr Ser Ser Thr Ser Asn

65 70 75 80

Ser Asn Ser Thr Lys Arg Val Thr Arg Val Val His Arg Leu Arg Asn

85 90 95

Pro Met Arg Leu Gly Met Ala Arg Arg Ser Val Gly Glu Arg Gln Ala

100 105 110

Glu Lys Leu Ala Lys Pro Leu Gly Phe Ser Leu Ala Ala Phe Ala Asn

115 120 125

Met Val Ile Ala Arg Lys Asn Ala Ala Gly Gln Asn Val Tyr Val Asp

130 135 140

Asp Leu Val Glu Ile Phe Ala Thr Leu Val Glu Glu Ser Leu Ala Asn

145 150 155 160

Val Tyr Gly Asn Lys Leu Gly Ser Phe Ala Thr Asn Phe Glu Gln Thr

165 170 175

Phe Ser Ser Thr Leu Lys Ile Leu Lys Leu Thr Asn Glu Cys Ala Asn

180 185 190

Pro His Gln Ser Asn Asn Asn Asp Gly Gly Ser Cys Asn Leu Asp Arg

195 200 205

Ser Thr Ile Asp Gly Cys Ser Asp Thr Glu Leu Phe Glu Arg Glu Thr

210 215 220

Ser Ser Ala Thr Ser Ala Tyr Glu Val Met Gln Gly Ser Ala Thr Ala

225 230 235 240

Thr Ser Leu Met Asn Glu Leu Ala Leu Phe Glu Glu Thr Leu Gln Leu

245 250 255

Ser Cys Val Pro Pro Arg Ser Ser Ala Met Ala Leu Thr Thr Asp Glu

260 265 270

Arg Phe Leu Lys Glu Gln Thr Arg Ala Asn Asp Leu Lys Thr Val Glu

275 280 285

Ile Gly Leu Gln Ile Arg Glu Leu Arg Cys Lys Glu Thr Ala Leu Gly

290 295 300

Leu Lys Phe Glu Ser Asn Asn Leu Gly Lys Ala Ala Leu Glu Leu Asp

305 310 315 320

Val Ser Lys Ala Ala Phe Arg Ala Glu Lys Phe Lys Thr Glu Leu Glu

325 330 335

Asp Thr Arg Lys Glu Glu Met Val Thr Arg Ile Met Asp Trp Leu Leu

340 345 350

Val Ser Val Phe Ser Met Leu Ala Ser Met Val Leu Gly Val Tyr Asn

355 360 365

Phe Ser Ile Lys Arg Ile Glu Asp Ala Thr Ser Val Cys Asp Gln Ser

370 375 380

Glu Glu Lys Ser Ser Ser Trp Trp Val Pro Lys Gln Val Ser Ser Ile

385 390 395 400

Asn Ser Gly Phe Asn Thr Phe Ile Cys Arg Val Arg Val Trp Val Gln

405 410 415

Ile Phe Phe Gly Val Leu Met Ile Ile Val Phe Thr Tyr Phe Leu Asn

420 425 430

Lys Arg Ser Ser Gly Thr Lys Gln Thr Met Pro Ile Ser Phe Ile Val

435 440 445

Leu Phe Leu Gly Ile Phe Cys Gly Val Ser Gly Lys Leu Cys Val Asp

450 455 460

Thr Leu Gly Gly Asp Gly Lys Leu Trp Leu Ile Val Trp Glu Val Phe

465 470 475 480

Cys Leu Leu Gln Phe Val Ala Asn Val Phe Thr Leu Ala Leu Tyr Gly

485 490 495

Leu Met Phe Gly Pro Ile Asn Val Thr Gln Glu Thr Arg Ser Asn Arg

500 505 510

Cys Asn Ser Met Phe Pro Tyr Trp Ala Arg Arg Ser Val Val Tyr Val

515 520 525

Val Ile Leu Phe Val Leu Pro Val Ile Asn Gly Leu Leu Pro Phe Ala

530 535 540

Thr Phe Gly Glu Trp Arg Asp Phe Ala Met Tyr His Leu His Gly Gly

545 550 555 560

Ser Asp Tyr Ala

<210> 109

<211> 1668

<212> DNA

<213> Glycine max

<400> 109

atggctgaga ttcagaattg ttcctcggaa ccagaaccaa ccgcaatcaa cgaatgggag 60

ggtcaagctt cgatgaagag cacaacgcaa agttcagaag cctccgatac gacgtcgtca 120

cgaaaagaga ataaaggtaa aggtaaggct gcggcgttca aacgacggaa ccctagagtt 180

cttgttcgcc gccatagggc taataatgtg gacactattg gccttcctct tggcatgtca 240

ttcgccgctg ttatggctca ggtgatgtat agaagagatg tagcagccga gagtatgtct 300

cccagtcatc tttcgatgat gtgttcatcg gctatcaaag aatctcttgc cagtgttttt 360

ggagacaagc tagatggttt gacgaggaac tttgaacaat ctttttgtag caccttgagt 420

actcttcagt taatttatga atcatccaag agcaatgaag gaaataaatt aaataataca 480

aagatggaaa ttatgagttc taaattgact ctcaataaag aggaatgctc aggtgatatt 540

gttacagagg tcggtcattc aagacatgct gaaatccaag atcaatcaat tagtcatgat 600

tctcctgaag agagcaggga taactttcat atgggttcag ttagtcgcga tctcactttg 660

tatgggcaat caaaccaaat ggtttccttt tctcaaatat cttttggatc tgtaaataat 720

cctatggtta gtatttttga gaagtccatc atggagcagt gtcgttctaa tgacctcaag 780

acacttgaac ttggccttaa aatgaaggaa ttgaaattga aagaggatga attggctctc 840

aaccttgatt cgaataattt aaacagatca aaattagtca tgggagaatc aaagcaatct 900

ttcaaagagg aaaagttcaa gacccagtta gaagatacga ggcatggtga actcaaaaag 960

aagtgcattg actgtcttat tactggtttg cttatcatgt catcctcact tttgtatggt 1020

gcctatgttt attcctacga gcggattact aaagctactg aatcatgtac accatcaacc 1080

caggcaatgg ttgtgtctgc atactactcc agcattgcct ttgctcaatt ggcagttgaa 1140

tcttcctcct ggtggactcc caagtcaatg gtcttattca attcaaagtt gcatattttg 1200

tggtgtcaag ttcaagttat gagccgaatg gcatttggca tcttgatgat ttttgctgtt 1260

gcatatttgc tcttgcagcg gtcgaccaca acatcacaga ctatgccagt cactttcatc 1320

cttttaatgt tgggaatttt ttgtggctat tgtggcaagc tatgtgtaga gacattgggt 1380

gggagtggta ttgtgtggct cttatactgg gagattatgt gcttgctgca tttcttatct 1440

ctttgctgga catccgcttt gttccggatc cttcatggac cagtcactcc atcacaaaca 1500

atggaggaga atacaattct tcggtattgg attcgcagag ttctgttcta tgctgctatg 1560

cttgtgtttc taccgttgtt ttgtggtctc atgccatttg ctagcctagg tcaatggaaa 1620

gaacatttta cattgaaggg gtcagatttt aatggatccg aatggtaa 1668

<210> 110

<211> 555

<212> PRT

<213> Glycine max

<400> 110

Met Ala Glu Ile Gln Asn Cys Ser Ser Glu Pro Glu Pro Thr Ala Ile

1 5 10 15

Asn Glu Trp Glu Gly Gln Ala Ser Met Lys Ser Thr Thr Gln Ser Ser

20 25 30

Glu Ala Ser Asp Thr Thr Ser Ser Arg Lys Glu Asn Lys Gly Lys Gly

35 40 45

Lys Ala Ala Ala Phe Lys Arg Arg Asn Pro Arg Val Leu Val Arg Arg

50 55 60

His Arg Ala Asn Asn Val Asp Thr Ile Gly Leu Pro Leu Gly Met Ser

65 70 75 80

Phe Ala Ala Val Met Ala Gln Val Met Tyr Arg Arg Asp Val Ala Ala

85 90 95

Glu Ser Met Ser Pro Ser His Leu Ser Met Met Cys Ser Ser Ala Ile

100 105 110

Lys Glu Ser Leu Ala Ser Val Phe Gly Asp Lys Leu Asp Gly Leu Thr

115 120 125

Arg Asn Phe Glu Gln Ser Phe Cys Ser Thr Leu Ser Thr Leu Gln Leu

130 135 140

Ile Tyr Glu Ser Ser Lys Ser Asn Glu Gly Asn Lys Leu Asn Asn Thr

145 150 155 160

Lys Met Glu Ile Met Ser Ser Lys Leu Thr Leu Asn Lys Glu Glu Cys

165 170 175

Ser Gly Asp Ile Val Thr Glu Val Gly His Ser Arg His Ala Glu Ile

180 185 190

Gln Asp Gln Ser Ile Ser His Asp Ser Pro Glu Glu Ser Arg Asp Asn

195 200 205

Phe His Met Gly Ser Val Ser Arg Asp Leu Thr Leu Tyr Gly Gln Ser

210 215 220

Asn Gln Met Val Ser Phe Ser Gln Ile Ser Phe Gly Ser Val Asn Asn

225 230 235 240

Pro Met Val Ser Ile Phe Glu Lys Ser Ile Met Glu Gln Cys Arg Ser

245 250 255

Asn Asp Leu Lys Thr Leu Glu Leu Gly Leu Lys Met Lys Glu Leu Lys

260 265 270

Leu Lys Glu Asp Glu Leu Ala Leu Asn Leu Asp Ser Asn Asn Leu Asn

275 280 285

Arg Ser Lys Leu Val Met Gly Glu Ser Lys Gln Ser Phe Lys Glu Glu

290 295 300

Lys Phe Lys Thr Gln Leu Glu Asp Thr Arg His Gly Glu Leu Lys Lys

305 310 315 320

Lys Cys Ile Asp Cys Leu Ile Thr Gly Leu Leu Ile Met Ser Ser Ser

325 330 335

Leu Leu Tyr Gly Ala Tyr Val Tyr Ser Tyr Glu Arg Ile Thr Lys Ala

340 345 350

Thr Glu Ser Cys Thr Pro Ser Thr Gln Ala Met Val Val Ser Ala Tyr

355 360 365

Tyr Ser Ser Ile Ala Phe Ala Gln Leu Ala Val Glu Ser Ser Ser Trp

370 375 380

Trp Thr Pro Lys Ser Met Val Leu Phe Asn Ser Lys Leu His Ile Leu

385 390 395 400

Trp Cys Gln Val Gln Val Met Ser Arg Met Ala Phe Gly Ile Leu Met

405 410 415

Ile Phe Ala Val Ala Tyr Leu Leu Leu Gln Arg Ser Thr Thr Thr Ser

420 425 430

Gln Thr Met Pro Val Thr Phe Ile Leu Leu Met Leu Gly Ile Phe Cys

435 440 445

Gly Tyr Cys Gly Lys Leu Cys Val Glu Thr Leu Gly Gly Ser Gly Ile

450 455 460

Val Trp Leu Leu Tyr Trp Glu Ile Met Cys Leu Leu His Phe Leu Ser

465 470 475 480

Leu Cys Trp Thr Ser Ala Leu Phe Arg Ile Leu His Gly Pro Val Thr

485 490 495

Pro Ser Gln Thr Met Glu Glu Asn Thr Ile Leu Arg Tyr Trp Ile Arg

500 505 510

Arg Val Leu Phe Tyr Ala Ala Met Leu Val Phe Leu Pro Leu Phe Cys

515 520 525

Gly Leu Met Pro Phe Ala Ser Leu Gly Gln Trp Lys Glu His Phe Thr

530 535 540

Leu Lys Gly Ser Asp Phe Asn Gly Ser Glu Trp

545 550 555

<210> 111

<211> 945

<212> DNA

<213> Oryza sativa

<400> 111

atgctacaca agtctatccc taacggcggc cggatcgcac gccgtatcat ccccacccac 60

tgcgacggcc ttgtcgccgt cgccacctgc ggcggggcca cgttcgtgtg caacccggcc 120

acccaggagc tcgtcgtgtt gccgcccggc accagcggcc gcagccgccg cggcccgtcc 180

ccagtgtcta ccgaatcgac ggcggcgatc ggcttcgatc cgtggcggaa caggtacgtc 240

gtcgccagat gcttctacta ccgcaagtcc ggcaaccact atccgccggt ctacaacgtc 300

gggcacgaga tcttcacgct cggcggcggc gccggcgacg gatggctggc ggactgcagg 360

acccgccgcg cgccatcagc cccgacggga gacccgccgc ctgcacgcgc ggcggcgggg 420

cctccttcta ctggttcatc gacgagcatg agccgtgcgc gctgctgcgg ttcagcctgc 480

gggacgaggc gttcgacgtg gtccccgtgc cccccgggct gcacgggctt cacgtacgac 540

gaccgcctgg cggacctcgc cggcgagctg tgctacgtgc atcgtgtccg cacgggggtt 600

gccacccacg aagtttggat ggcggcggcg gcggtcgacg acgacgagcc ggagtggtgg 660

ctgcgatacc gagtggacct gtgggggtac gcgtggggct tggtcgccgg cgaacgctgg 720

ttccacagct tcggcgccac ggccggcgat gacggcgtgg acgaggaggc gacgttggtg 780

accatgttgt acaaggagct gtgctggcac agggagcgga gcaaaccggt ggtgaaagac 840

gtgaacgtgc gagggagccg gtacagctgt gagccgactc cgactataca ccatgtcatc 900

cgttacgtgg agagcctcgt ctccattaca gcacctaact actga 945

<210> 112

<211> 314

<212> PRT

<213> Oryza sativa

<400> 112

Met Leu His Lys Ser Ile Pro Asn Gly Gly Arg Ile Ala Arg Arg Ile

1 5 10 15

Ile Pro Thr His Cys Asp Gly Leu Val Ala Val Ala Thr Cys Gly Gly

20 25 30

Ala Thr Phe Val Cys Asn Pro Ala Thr Gln Glu Leu Val Val Leu Pro

35 40 45

Pro Gly Thr Ser Gly Arg Ser Arg Arg Gly Pro Ser Pro Val Ser Thr

50 55 60

Glu Ser Thr Ala Ala Ile Gly Phe Asp Pro Trp Arg Asn Arg Tyr Val

65 70 75 80

Val Ala Arg Cys Phe Tyr Tyr Arg Lys Ser Gly Asn His Tyr Pro Pro

85 90 95

Val Tyr Asn Val Gly His Glu Ile Phe Thr Leu Gly Gly Gly Ala Gly

100 105 110

Asp Gly Trp Leu Ala Asp Cys Arg Thr Arg Arg Ala Pro Ser Ala Pro

115 120 125

Thr Gly Asp Pro Pro Pro Ala Arg Ala Ala Ala Gly Pro Pro Ser Thr

130 135 140

Gly Ser Ser Thr Ser Met Ser Arg Ala Arg Cys Cys Gly Ser Ala Cys

145 150 155 160

Gly Thr Arg Arg Ser Thr Trp Ser Pro Cys Pro Pro Gly Cys Thr Gly

165 170 175

Phe Thr Tyr Asp Asp Arg Leu Ala Asp Leu Ala Gly Glu Leu Cys Tyr

180 185 190

Val His Arg Val Arg Thr Gly Val Ala Thr His Glu Val Trp Met Ala

195 200 205

Ala Ala Ala Val Asp Asp Asp Glu Pro Glu Trp Trp Leu Arg Tyr Arg

210 215 220

Val Asp Leu Trp Gly Tyr Ala Trp Gly Leu Val Ala Gly Glu Arg Trp

225 230 235 240

Phe His Ser Phe Gly Ala Thr Ala Gly Asp Asp Gly Val Asp Glu Glu

245 250 255

Ala Thr Leu Val Thr Met Leu Tyr Lys Glu Leu Cys Trp His Arg Glu

260 265 270

Arg Ser Lys Pro Val Val Lys Asp Val Asn Val Arg Gly Ser Arg Tyr

275 280 285

Ser Cys Glu Pro Thr Pro Thr Ile His His Val Ile Arg Tyr Val Glu

290 295 300

Ser Leu Val Ser Ile Thr Ala Pro Asn Tyr

305 310

Claims

1.一种分离的多核苷酸，其包含编码多肽的多核苷酸，该多肽的氨基酸序列至少与SEQID NO:3、6、9、12、15、18、21或24具有90％的序列同一性，其中多核苷酸在植物中的增加表达可增加植物的耐旱性和产量。

2.权利要求1所述的分离的多核苷酸，其中所述多核苷酸包含的核苷酸序列为SEQ IDNO:1、SEQ ID NO:2、SEQ ID NO:4、SEQ ID NO:5、SEQ ID NO:7、SEQ ID NO:8、SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:13、SEQ ID NO:14、SEQ ID NO:16、SEQ ID NO:17、SEQ IDNO:19、SEQ ID NO:20、SEQ ID NO:22或SEQ ID NO:23。

3.权利要求1所述的分离的多核苷酸，其中所述编码的多肽包含的氨基酸序列为SEQID NO:3、SEQ ID NO:6、SEQ ID NO:9、SEQ ID NO:12、SEQ ID NO:15、SEQ ID NO:18、SEQ IDNO:21、或SEQ ID NO:24。

4.权利要求1-3所述的任意的分离的多核苷酸，提高植物中所述多核苷酸的表达能增加在干旱条件下耐旱性和/或籽粒产量。

5.一种重组DNA构建体，包含权利要求1-3所述的任一分离的多核苷酸，可操作地连接至少一个异源调控元件。

6.权利要求5所述的重组DNA构建体，其中所述的调控元件是异源启动子。

7.一种改良的植物或种子，包含有至少一个多核苷酸编码多肽的表达的增加，所述多肽包含的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21或24有至少90％的序列一致性。

8.权利要求7所述的植物，所述植物在其基因组中包含有一种重组DNA构建体。所述重组DNA构建体包含有权利要求1-3中任一多核苷酸，并可操作地连接有至少一个调控元件。其中所述植物与对照植物相比较时表现出增加的耐旱性。

9.权利要求7所述的植物，其中所述植物在其基因组位点上包含有一个靶向基因修饰，所述靶向基因修饰包含的多核苷酸序列能编码一个多肽，所述多肽的氨基酸序列与SEQ IDNO:3、6、9、12、15、18、21或24有至少90％的序列一致性。因此增加所述多肽的表达，其中所述植物在和对照植物相比较时表现出增加的耐旱性。

10.根据任一权利要求7-9中所述的植物，其中所述植物选自水稻、玉米、大豆、向日葵、高粱、油菜、小麦、苜蓿、棉花、大麦、小米、甘蔗和柳枝稷。

11.一种提高植物耐旱性的方法，包括增加编码多肽的至少一个多核苷酸的表达，该多肽包含的核苷酸序列与SEQ ID NO:3、6、9、12、15、18、21或24有至少90％的序列一致性。

12.根据权利要求11所述的方法，其中所述方法包括：

a)在可再生植物细胞中表达一个重组DNA构建体，所述重组DNA构建体包含一个调控元件可操作地连接所述多核苷酸序列；和

b)产生所述植物，其中所述植物在其基因组中包含所述重组DNA构建体。

13.根据权利要求11所述的方法，其中所述方法包括：

a)在可再生植物细胞的基因组位点上引入一个靶向基因修饰，所述靶向基因修饰编码多肽的氨基酸序列与SEQ ID NO:3、6、9、12、15、18、21或24有至少90％的序列一致性；和

b)产生所述植物，其中所述多肽的水平和/或活性在所述植物中是增加的。

14.根据权利要求13所述的方法，其中所述靶向基因修饰是使用以下基因组修饰技术引入的：多核苷酸引导的核酸内切酶、CRISPR-Cas核酸内切酶、碱基编辑脱氨酶、锌指核酸酶、转录激活物样效应器核酸酶(TALEN)，工程化位点特异性巨核酸酶，或Argonaute。

15.根据权利要求13所述的方法，其中所述靶向基因修饰存在于(a)所述编码区中；(b)非编码区；(c)调控序列；(d)未翻译区；或(e)编码多肽的基因组位点(a)-(d)的任何组合，所述多肽包含的氨基酸序列与SEQ ID NO：3、6、9、12、15、18、21和24这些序列有至少90％的序列一致性。

16.权利要求12所述的方法，其中该调控元件是异源启动子。