CN114174516A - 制备抗原结合单元的方法 - Google Patents

制备抗原结合单元的方法 Download PDF

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CN114174516A
CN114174516A CN202180002029.1A CN202180002029A CN114174516A CN 114174516 A CN114174516 A CN 114174516A CN 202180002029 A CN202180002029 A CN 202180002029A CN 114174516 A CN114174516 A CN 114174516A
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abu
antigen
less
cells
days
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谢晓亮
曹云龙
孙文洁
张旭
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Peking University
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1002Coronaviridae
    • C07K16/1003Severe acute respiratory syndrome coronavirus 2 [SARS‐CoV‐2 or Covid-19]
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/70Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/90Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
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  • Virology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pulmonology (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

一种制备抗体的方法,涉及免疫学领域和分子病毒学领域,特别是新型冠状病毒的诊断、预防和治疗领域。具体而言,涉及抗新型冠状病毒的单克隆抗体,以及包含所述抗体的组合物(例如,诊断剂和治疗剂)。此外还涉及所述抗体的制备和筛选以及用途。

Description

PCT国内申请,说明书已公开。

Claims (27)

  1. PCT国内申请,权利要求书已公开。
CN202180002029.1A 2020-05-13 2021-05-12 制备抗原结合单元的方法 Pending CN114174516A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2020104038732 2020-05-13
CN202010403873 2020-05-13
PCT/CN2021/093305 WO2021228135A1 (zh) 2020-05-13 2021-05-12 制备抗原结合单元的方法

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CN114174516A true CN114174516A (zh) 2022-03-11

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US (1) US20230183322A1 (zh)
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WO2024096742A1 (en) * 2022-11-06 2024-05-10 Leyden Laboratories B.V. SARS-CoV-2 BINDING POLYPEPTIDE

Citations (3)

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Publication number Priority date Publication date Assignee Title
CN110951756A (zh) * 2020-02-23 2020-04-03 广州恩宝生物医药科技有限公司 表达SARS-CoV-2病毒抗原肽的核酸序列及其应用
CN110974950A (zh) * 2020-03-05 2020-04-10 广州恩宝生物医药科技有限公司 一种用于预防SARS-CoV-2感染的腺病毒载体疫苗
CN111074007A (zh) * 2020-02-15 2020-04-28 上海迪飞医学检验实验室有限公司 一种检测sars-cov-2病毒的恒温扩增试剂盒及引物探针组

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Publication number Priority date Publication date Assignee Title
US8563305B2 (en) * 2009-04-30 2013-10-22 Oklahoma Medical Research Foundation Rapid generation of antibodies
US11384354B2 (en) * 2015-12-21 2022-07-12 Zumutor Biologics Inc. Method of generating an antibody naïve library, said library and application(s) thereof
CN109666070B (zh) * 2017-10-13 2021-02-19 清华大学 单克隆抗体mers-4v2及其编码基因和应用
CN107760690B (zh) * 2017-10-25 2018-08-28 珠海泰诺麦博生物技术有限公司 一种高通量全人源抗体的制备方法及应用
CN108752472B (zh) * 2018-06-13 2022-03-22 中国科学院广州生物医药与健康研究院 一种抗h7n9抗体及其制备方法和应用

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111074007A (zh) * 2020-02-15 2020-04-28 上海迪飞医学检验实验室有限公司 一种检测sars-cov-2病毒的恒温扩增试剂盒及引物探针组
CN110951756A (zh) * 2020-02-23 2020-04-03 广州恩宝生物医药科技有限公司 表达SARS-CoV-2病毒抗原肽的核酸序列及其应用
CN110974950A (zh) * 2020-03-05 2020-04-10 广州恩宝生物医药科技有限公司 一种用于预防SARS-CoV-2感染的腺病毒载体疫苗

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CN113667011B (zh) 2024-03-29
CN113667011A (zh) 2021-11-19
US20230183322A1 (en) 2023-06-15
WO2021228135A1 (zh) 2021-11-18

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Application publication date: 20220311