CN114149946B - Klebsiella and application thereof - Google Patents
Klebsiella and application thereof Download PDFInfo
- Publication number
- CN114149946B CN114149946B CN202111455855.XA CN202111455855A CN114149946B CN 114149946 B CN114149946 B CN 114149946B CN 202111455855 A CN202111455855 A CN 202111455855A CN 114149946 B CN114149946 B CN 114149946B
- Authority
- CN
- China
- Prior art keywords
- klebsiella pneumoniae
- klebsiella
- desulfurization
- sewage treatment
- strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000588748 Klebsiella Species 0.000 title abstract description 10
- 241000588747 Klebsiella pneumoniae Species 0.000 claims abstract description 46
- 239000010865 sewage Substances 0.000 claims abstract description 22
- 238000004321 preservation Methods 0.000 claims abstract description 13
- -1 sulfide ions Chemical class 0.000 claims abstract description 8
- 238000009629 microbiological culture Methods 0.000 claims abstract description 7
- 230000001580 bacterial effect Effects 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 12
- 238000011081 inoculation Methods 0.000 claims description 8
- 230000001877 deodorizing effect Effects 0.000 claims description 3
- 230000003009 desulfurizing effect Effects 0.000 claims description 2
- 239000002351 wastewater Substances 0.000 claims 1
- 238000006477 desulfuration reaction Methods 0.000 abstract description 23
- 230000023556 desulfurization Effects 0.000 abstract description 23
- 238000000034 method Methods 0.000 abstract description 14
- 238000010276 construction Methods 0.000 abstract description 4
- 229910052979 sodium sulfide Inorganic materials 0.000 abstract description 4
- GRVFOGOEDUUMBP-UHFFFAOYSA-N sodium sulfide (anhydrous) Chemical compound [Na+].[Na+].[S-2] GRVFOGOEDUUMBP-UHFFFAOYSA-N 0.000 abstract description 4
- 241000894006 Bacteria Species 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 17
- 230000000052 comparative effect Effects 0.000 description 10
- 239000002054 inoculum Substances 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 5
- 230000001105 regulatory effect Effects 0.000 description 5
- 239000011259 mixed solution Substances 0.000 description 4
- 239000010802 sludge Substances 0.000 description 4
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 108020004465 16S ribosomal RNA Proteins 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000004332 deodorization Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
Abstract
The invention relates to the technical field of desulfurization bacteria. The invention provides a Klebsiella and application thereof, wherein the Klebsiella has the preservation name of Klebsiella pneumoniae HZ1, latin is Klebsiella pneumoniae, and the preservation date is: 2021, 9, 16, deposit unit: china general microbiological culture Collection center, accession number: CGMCC No.23424. The sodium sulfide solution with the concentration of 800mg/L is treated by the klebsiella pneumoniae, the removal rate of sulfide ions is up to 89.27%, and malodorous substances generated in the sewage treatment process can be greatly reduced. The desulfurization conditions required by the strain are easy to realize and control, and the strain has strong desulfurization capability and good application prospect. Can efficiently deodorize, can reduce the odor treatment cost, and has positive significance for the construction and development of sewage treatment plants in cities.
Description
Technical Field
The invention relates to the technical field of desulfurization bacteria, in particular to klebsiella and application thereof.
Background
The urban sewage treatment plant is an important municipal and environmental protection infrastructure, and plays a very important role in ecological environment protection, water resource conservation and the like. Along with the rapid development of socioeconomic and the continuous acceleration of urban process, the urban scale is rapidly expanded, and the unordered development of the periphery of the sewage treatment plant is added, the sewage treatment plant which is built in the downstream and suburban areas of the city in early stage is gradually surrounded by the city, and foul gas is inquiredThe problems affect the peripheral production and life, and seriously affect the sewage plants to play a positive role in urban construction and development. It is reported that malodor emitted from urban sewage treatment plants is mainly caused by H 2 S is discharged to cause H 2 S and sulfur-containing compounds can account for 80-90% of malodorous compounds, and the concentration of hydrogen sulfide can be as high as 0.145mg/L.
The source deodorizing technology of sewage treatment plants is an ideal malodor control technology. In addition, in the sewage treatment process, in order to fully play the role of microorganisms in the activated sludge, part of the sludge-water mixture in the biochemical treatment link is returned to the front end for recycling, and the returned activated sludge generally has the potential of biologically oxidizing most compounds causing odor to be harassment, but the high-efficiency desulfurization strains found in the returned activated sludge are fewer at present, and the realization of large-scale application of the deodorizing technology from the source of the returned sludge is still far.
Disclosure of Invention
The invention aims to provide a Klebsiella and application thereof, which can remove odor generated in the sewage treatment process from the source.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a Klebsiella pneumoniae, wherein the Klebsiella pneumoniae has a preservation name of Klebsiella pneumoniae HZ1, latin is Klebsiella pneumoniae, and the preservation date is as follows: 2021, 9, 16, deposit unit: china general microbiological culture Collection center, accession number: cgmccno.23424.
Preferably, the 16S rDNA sequencing result of Klebsiella pneumoniae is shown as SEQ ID No. 1.
The invention also provides application of the klebsiella pneumoniae in sewage desulfurization and deodorization.
Preferably, the klebsiella pneumoniae liquid is inoculated into the sewage when the klebsiella pneumoniae is used.
Preferably, the inoculation ratio of Klebsiella pneumoniae bacterial liquid at the time of inoculation is 4-13% (v/v%).
Preferably, the klebsiella pneumoniaeThe bacterial density in the bacterial liquid of the bacillus is 2 to 4 multiplied by 10 8 And each mL.
Preferably, the temperature of the klebsiella pneumoniae in the use process is 15-30 ℃ and the pH is 6-8.
Preferably, the concentration of the sulfide ions in the sewage is 800-1000 mg/L.
The invention provides a Klebsiella and application thereof, wherein the Klebsiella has a preservation name of Klebsiella pneumoniae HZ1, latin is Klebsiella pneumoniae, and the preservation date is as follows: 2021, 9, 16, deposit unit: china general microbiological culture Collection center, accession number: CGMCC No.23424. The sodium sulfide solution with the concentration of 800mg/L is treated by the klebsiella pneumoniae, the removal rate of sulfide ions is up to 89.27%, and malodorous substances generated in the sewage treatment process can be greatly reduced. The desulfurization conditions required by the strain are easy to realize and control, and the strain has strong desulfurization capability and good application prospect. Can efficiently deodorize, can reduce the odor treatment cost, and has positive significance for the construction and development of sewage treatment plants in cities.
Preservation description
Klebsiella pneumoniae HZ1, latin is Klebsiella pneumoniae, and the strain is preserved in China general microbiological culture Collection center (China Committee) with the address: no. 3 of north chen west way 1, the collection date is 2021, 9 months and 16 days, and the collection number is: CGMCC No.23424.
Drawings
FIG. 1 shows desulfurization rate results of examples 1 to 3 and comparative examples 1 to 6.
Detailed Description
The invention provides a Klebsiella pneumoniae, wherein the Klebsiella pneumoniae has a preservation name of Klebsiella pneumoniae HZ1, latin is Klebsiella pneumoniae, and the preservation date is as follows: 2021, 9, 16, deposit unit: china general microbiological culture Collection center, accession number: CGMCC No.23424.
In the invention, the 16S rDNA sequencing result of the Klebsiella pneumoniae is shown as SEQ ID NO. 1.
The invention also provides application of the klebsiella pneumoniae in sewage desulfurization and deodorization.
In the present invention, the klebsiella pneumoniae is preferably used by inoculating a klebsiella pneumoniae liquid into sewage.
In the present invention, the inoculation ratio of Klebsiella pneumoniae bacterial liquid at the time of inoculation is preferably 4 to 13% (v/v%), more preferably 7 to 10% (v/v%), still more preferably 8 to 9% (v/v%).
In the present invention, the bacterial density in the Klebsiella pneumoniae bacterial liquid is preferably 2 to 4×10 8 Each mL, more preferably 3X 10 8 And each mL.
In the present invention, the temperature of the Klebsiella pneumoniae is preferably 15 to 30℃and more preferably 20 to 25℃during use.
In the present invention, the pH of the klebsiella pneumoniae during use is preferably 6 to 8, more preferably 6.5 to 7.5, and even more preferably 7.33.
In the present invention, the concentration of the sulfide ion in the sewage is preferably 800 to 1000mg/L, more preferably 850 to 950mg/L, and still more preferably 900mg/L.
In the present invention, the time for use of the klebsiella pneumoniae in the use process is preferably 100 to 140 minutes, more preferably 110 to 130 minutes, and even more preferably 120 minutes.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
The materials used in the examples are as follows:
1. enrichment medium: glucose 5.0g/L, na 2 S·9H 2 O 5g/L；KH 2 PO 4 1.0 g/L、 MgCl 2 ·6H 2 O 0.8g/L、K 2 HPO 4 1.0 g/L、NH 4 Cl 0.4g/L、FeSO 4 ·7H 2 O0.01 g/L, water is used as solvent, pH is adjusted to 7.33 before use, and sterilization is carried out for 30min at 121 ℃.
2. Preparing bacterial liquid: klebsiella pneumoniae HZ1 is inoculated into a 250 mL conical flask filled with 100mL of enrichment medium, and is placed in a shaking table for culture at 30 ℃ and 160r/min for shake culture for 5d.
Example 1
And 5mL of bacterial liquid which is subjected to shake culture for 3d in an enrichment culture medium is taken, placed in a solution filled with 100mL of sodium sulfide with the concentration of 800mg/L, added with a rotor, placed on a constant-temperature magnetic stirrer for stirring, the temperature of the solution is regulated to be 30 ℃, the rotating speed is 1000r/min, and the mass of the sulfide ions in the solution is sampled and detected after 120min, and the desulfurization rate is calculated.
Comparative example 1
The procedure was the same as in example 1, except that the temperature of the solution was adjusted to 15 ℃.
Comparative example 2
The procedure was the same as in example 1, except that the temperature of the solution was adjusted to 25 ℃.
Example 2
And 5mL of bacterial liquid which is subjected to shake culture for 3d in an enrichment culture medium is taken, placed in a mixed solution filled with 100mL of sulfide solution with the concentration of 800mg/L, added with a rotor, placed on a constant-temperature magnetic stirrer for stirring, the temperature of the solution is regulated to 35 ℃, the pH value is regulated to 7.33, the rotating speed is 1000r/min, and the mass of sulfide ions in the solution is sampled and detected after 120min, so that the desulfurization rate is calculated.
Comparative example 3
The procedure was the same as in example 2, except that the pH of the solution was adjusted to 6.56.
Comparative example 4
The procedure was the same as in example 2, except that the pH of the solution was adjusted to 8.09.
Example 3
15mL of bacterial liquid which is subjected to shake culture for 3d in an enrichment culture medium is placed in a mixed solution containing 100mL of sulfide solution with the concentration of 800mg/L (the inoculation ratio is 13%), a rotor is added into the mixed solution, the mixed solution is placed on a constant-temperature magnetic stirrer for stirring, the temperature of the solution is regulated to 35 ℃, the pH value is regulated to 7.33, the rotating speed is 1000r/min, and the mass of the sulfide ions in the solution is sampled and detected after 120min, and the desulfurization rate is calculated.
Comparative example 5
The procedure of example 3 was followed except that the inoculum size of the culture medium for enrichment culture for 3d was 5mL (inoculation ratio: 5%).
Comparative example 6
The procedure of example 3 was repeated except that the inoculum size of the culture medium for enrichment culture for 3d was 10mL (inoculum size: 9%).
Conclusion: the results are shown in Table 1.
TABLE 1
The results showed that in example 1, the desulfurization rate was 80.47% at a temperature of 30℃and that in comparative examples 1 and 2, i.e., at temperatures of 15 and 25℃respectively, the desulfurization rates were slightly reduced to 74.67% and 77.57% respectively. In example 2, the desulfurization rate was 82.18% when the pH was 7.33, i.e., near neutral, whereas in comparative examples 3 and 4, i.e., when the pH was 6.56 and 8.09, respectively, the desulfurization rates were 77.18% and 79.32%, respectively, slightly decreased. In example 3, the desulfurization rate was 89.27% when the inoculum size was 15mL, i.e., the inoculation ratio was 13% (v/v%), while the desulfurization rates were slightly decreased, 86.19% and 79.27% when the inoculum sizes were 5% and 9% (v/v) in comparative examples 5 and 6, respectively, and the statistical results are shown in FIG. 1.
In summary, when the temperature is about 30℃and the pH is about 7 (i.e., neutral condition) and the inoculum size is 13% (v/v%), the desulfurization effect of the above-mentioned desulfurizing bacteria is preferable, and the desulfurization rate is 89.27% at most.
From the above examples, the present invention provides a strain of klebsiella pneumoniae with the preservation name of klebsiella pneumoniae HZ1 and the preservation date of klebsiella pneumoniae, and uses thereof: 2021, 9, 16, deposit unit: china general microbiological culture Collection center, accession number: CGMCC No.23424. The sodium sulfide solution with the concentration of 800mg/L is treated by the klebsiella pneumoniae, the removal rate of sulfide ions is up to 89.27%, and malodorous substances generated in the sewage treatment process can be greatly reduced. The desulfurization conditions required by the strain are easy to realize and control, and the strain has strong desulfurization capability and good application prospect. Can efficiently deodorize, can reduce the odor treatment cost, and has positive significance for the construction and development of sewage treatment plants in cities.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Sequence listing
<110> Beijing university of forestry
West original environmental protection engineering (Shanghai) Co., ltd
<120> Klebsiella and use thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1404
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 1
gtagcgccct cccgaaggtt aagctaccta cttcttttgc acccactccc atggtgtgac 60
gggcggtgtg tacaaggccc gggaacgtat tcaccgtagc attctgatct acgattacta 120
gcgattccga cttcatggag tcgagttgca gactccaatc cggactacga catactttat 180
gaggtccgct tgctctcgcg aggtcgcttc tctttgtata tgccattgta gcacgtgtgt 240
agccctggtc gtaagggcca tgatgacttg acgtcatccc caccttcctc cagtttatca 300
ctggcagtct cctttgagtt cccggcctaa ccgctggcaa caaaggataa gggttgcgct 360
cgttgcggga cttaacccaa catttcacaa cacgagctga cgacagccat gcagcacctg 420
tctcacagtt cccgaaggca ccaaagcatc tctgctaagt tctgtggatg tcaagaccag 480
gtaaggttct tcgcgttgca tcgaattaaa ccacatgctc caccgcttgt gcgggccccc 540
gtcaattcat ttgagtttta accttgcggc cgtactcccc aggcggtcga tttaacgcgt 600
tagctccgga agccacgcct caagggcaca acctccaaat cgacatcgtt tacggcgtgg 660
actaccaggg tatctaatcc tgtttgctcc ccacgctttc gcacctgagc gtcagtcttt 720
gtccaggggg ccgccttcgc caccggtatt cctccagatc tctacgcatt tcaccgctac 780
acctggaatt ctacccccct ctacaagact ctagcctgcc agtttcgaat gcagttccca 840
ggttgagccc ggggatttca catccgactt gacagaccgc ctgcgtgcgc tttacgccca 900
gtaattccga ttaacgcttg caccctccgt attaccgcgg ctgctggcac ggagttagcc 960
ggtgcttctt ctgcgggtaa cgtcaatcga caaggttatt aaccttatcg ccttcctccc 1020
cgctgaaagt gctttacaac ccgaaggcct tcttcacaca cgcggcatgg ctgcatcagg 1080
cttgcgccca ttgtgcaata ttccccactg ctgcctcccg taggagtctg gaccgtgtct 1140
cagttccagt gtggctggtc atcctctcag accagctagg gatcgtcgcc taggtgagcc 1200
gttaccccac ctactagcta atcccatctg ggcacatctg atggcatgag gcccgaaggt 1260
cccccacttt ggtcttgcga catnatgcgg tattagctac cgtttccagt agttatcccc 1320
ctccatcagg cagtttccca gacattactc acccgtccgc cgctcgtcac ccgagagcaa 1380
gctctctgtg ctaccgctcg actg 1404
Claims (7)
1. The Klebsiella pneumoniae is characterized in that the Klebsiella pneumoniae has a preservation name of Klebsiella pneumoniae HZ1, and Latin is Klebsiella pneumoniae, and the preservation date is as follows: 2021, 9, 16, deposit unit: china general microbiological culture Collection center, accession number: CGMCC No.23424.
2. The use of klebsiella pneumoniae according to claim 1 in desulfurizing and deodorizing sewage.
3. The use according to claim 2, wherein the klebsiella pneumoniae is used by inoculating a klebsiella pneumoniae solution into the sewage.
4. The use according to claim 3, wherein the inoculation volume ratio of klebsiella pneumoniae bacterial liquid is 4-13%.
5. The use according to claim 4, wherein the bacterial density in the Klebsiella pneumoniae bacterial liquid is 2-4 x 10 8 And each mL.
6. The use according to claim 5, wherein the klebsiella pneumoniae has a temperature of 15-30 ℃ and a pH of 6-8 during use.
7. The use according to claim 6, wherein the concentration of sulfide ions in the wastewater is 800-1000 mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111455855.XA CN114149946B (en) | 2021-12-01 | 2021-12-01 | Klebsiella and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111455855.XA CN114149946B (en) | 2021-12-01 | 2021-12-01 | Klebsiella and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114149946A CN114149946A (en) | 2022-03-08 |
| CN114149946B true CN114149946B (en) | 2024-01-16 |
Family
ID=80455672
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111455855.XA Active CN114149946B (en) | 2021-12-01 | 2021-12-01 | Klebsiella and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114149946B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102409012A (en) * | 2011-10-19 | 2012-04-11 | 黑龙江大学 | Klebsiella pneumonia being separated from Termite gut |
| CN109055282A (en) * | 2018-09-27 | 2018-12-21 | 陕西科技大学 | One Klebsiella pneumoniae new strains and its separation method and application |
-
2021
- 2021-12-01 CN CN202111455855.XA patent/CN114149946B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102409012A (en) * | 2011-10-19 | 2012-04-11 | 黑龙江大学 | Klebsiella pneumonia being separated from Termite gut |
| CN109055282A (en) * | 2018-09-27 | 2018-12-21 | 陕西科技大学 | One Klebsiella pneumoniae new strains and its separation method and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114149946A (en) | 2022-03-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107512771B (en) | Siderite/sulfur biofilter and method for synchronously removing nitrogen and phosphorus in water by using siderite/sulfur biofilter | |
| CN110117567B (en) | Paracoccus denitrificans strain screening and application thereof in deodorization | |
| CN107699513B (en) | Black and odorous water body degrading bacterium and application thereof | |
| CN101941758A (en) | Method for treating nitrogen-containing wastewater from power plants by nitrifying bacteria immobilized on polyurethane | |
| JP2004248618A (en) | Bacterial group symbiotically living with fungus used for treating organic material and its application | |
| CN110078332B (en) | Method for promoting anaerobic fermentation of excess sludge to produce acid by using modified steel slag | |
| CN108018250A (en) | One plant of Acidithiobacillus ferrooxidans strain GF and its application in environmental improvement | |
| CN107603907B (en) | Bacillus aryabhattai GH-9 and application thereof | |
| CN111206027A (en) | Magnetic biochar suitable for loading bacillus and preparation method and application thereof | |
| CN107118982B (en) | Thiobacillus ferrooxidans and application thereof | |
| CN113003717A (en) | Device and process for enhancing denitrification anaerobic methane oxidation denitrification based on magnetite | |
| CN114149946B (en) | Klebsiella and application thereof | |
| CN116004459A (en) | Rhodococcus YZ-1 and application thereof in degrading organic pollutants | |
| CN112300961B (en) | Quinoline degrading strain and application thereof in quinoline-containing wastewater | |
| CN114230023A (en) | Method for treating sulfur-containing solid waste by microorganisms | |
| CN107384829B (en) | Application of bacillus megatherium YJB3 in artificial wetland water PAEs pollution purification | |
| CN112831429A (en) | Composite microbial inoculum for deodorization of sewage treatment plant and preparation process thereof | |
| CN107022494B (en) | Dictyophora pinicola BN-h1 and biological bacterium degradation agent as well as preparation method and application thereof | |
| CN111517603A (en) | Biological reduction treatment technology for expanded sludge | |
| CN111252896A (en) | Sulfur autotrophic material for removing nitrate from underground water and preparation method thereof | |
| CN110818180A (en) | Method for digesting black and odorous river channel by using microbial preparation | |
| CN114735825B (en) | Biochar loaded with mixed valence ferric oxide, preparation method and application thereof | |
| CN110104788B (en) | Mercury-containing wastewater treatment method and device | |
| CN117089502B (en) | Immobilized methane-oxidizing bacteria and immobilization method and application thereof | |
| JPH04277096A (en) | Method for treating organic waste water by photosynthesized microorganism |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Hong Yu Inventor after: Li Lihua Inventor after: Zhang Haoge Inventor after: Ju Qingling Inventor after: Cao Xiaoxin Inventor before: Hong Yu Inventor before: Li Lihua Inventor before: Zhang Haoge Inventor before: Ju Qingling Inventor before: Cao Xiaoxin |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 100083 No. 35 Qinghua East Road, Beijing, Haidian District Patentee after: BEIJING FORESTRY University Country or region after: China Patentee after: Xiyuan environmental protection (Shanghai) Co.,Ltd. Address before: 100083 No. 35 Qinghua East Road, Beijing, Haidian District Patentee before: BEIJING FORESTRY University Country or region before: China Patentee before: NISHIHARA ENVIRONMENT ENGINEERING (SHANGHAI) Co.,Ltd. |
|
| CP03 | Change of name, title or address |