CN114149946A - Klebsiella and application thereof - Google Patents
Klebsiella and application thereof Download PDFInfo
- Publication number
- CN114149946A CN114149946A CN202111455855.XA CN202111455855A CN114149946A CN 114149946 A CN114149946 A CN 114149946A CN 202111455855 A CN202111455855 A CN 202111455855A CN 114149946 A CN114149946 A CN 114149946A
- Authority
- CN
- China
- Prior art keywords
- klebsiella pneumoniae
- desulfurization
- preservation
- klebsiella
- sewage treatment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000588748 Klebsiella Species 0.000 title description 9
- 241000588747 Klebsiella pneumoniae Species 0.000 claims abstract description 47
- 238000006477 desulfuration reaction Methods 0.000 claims abstract description 25
- 230000023556 desulfurization Effects 0.000 claims abstract description 25
- 239000010865 sewage Substances 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 18
- 238000004321 preservation Methods 0.000 claims abstract description 18
- 229910052717 sulfur Inorganic materials 0.000 claims abstract description 10
- 239000011593 sulfur Substances 0.000 claims abstract description 10
- -1 sulfur ions Chemical class 0.000 claims abstract description 9
- 238000009629 microbiological culture Methods 0.000 claims abstract description 7
- 230000001580 bacterial effect Effects 0.000 claims description 19
- 239000007788 liquid Substances 0.000 claims description 17
- 238000011081 inoculation Methods 0.000 claims description 7
- 238000004332 deodorization Methods 0.000 claims description 5
- 108020004465 16S ribosomal RNA Proteins 0.000 claims description 3
- 239000002351 wastewater Substances 0.000 claims 2
- 229910052979 sodium sulfide Inorganic materials 0.000 abstract description 5
- GRVFOGOEDUUMBP-UHFFFAOYSA-N sodium sulfide (anhydrous) Chemical compound [Na+].[Na+].[S-2] GRVFOGOEDUUMBP-UHFFFAOYSA-N 0.000 abstract description 5
- 241000894006 Bacteria Species 0.000 abstract description 4
- 238000010276 construction Methods 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 19
- 230000000052 comparative effect Effects 0.000 description 11
- 239000002054 inoculum Substances 0.000 description 8
- 239000002609 medium Substances 0.000 description 6
- 239000010802 sludge Substances 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to the technical field of desulfurization bacteria. The invention provides a Klebsiella pneumoniae and application thereof, wherein the preservation name of the Klebsiella pneumoniae HZ1, the Latin is Klebsiella pneumoniae, and the preservation date is as follows: 16/9/2021, depository: china general microbiological culture Collection center, preservation number: CGMCC No. 23424. When 800mg/L sodium sulfide solution is treated by the Klebsiella pneumoniae of the invention, the removal rate of sulfur ions reaches 89.27 percent, and malodorous substances generated in the sewage treatment process can be greatly reduced. The desulfurization conditions required by the strain are easy to realize and control, and the strain has strong desulfurization capability and good application prospect. Not only can efficiently deodorize, but also can reduce the odor treatment cost, and has positive significance for the construction and development of sewage treatment plants in cities.
Description
Technical Field
The invention relates to the technical field of desulfurization bacteria, in particular to a Klebsiella and application thereof.
Background
The urban sewage treatment plant is an important municipal and environment-friendly infrastructure, and plays an important role in the aspects of ecological environment protection, water resource conservation and the like. With the rapid development of social economy and the continuous acceleration of urbanization process, the scale of cities is rapidly expanded, and the disordered development of the periphery of sewage treatment plants, the sewage treatment plants constructed in the downstream and suburbs of the cities in the early period are gradually surrounded by the cities, so that the problem of malodorous gas influences the production and life of the periphery, and the sewage treatment plants are seriously influenced to play a positive role in the construction and development of the cities. It is reported that the offensive odor emitted from municipal sewage treatment plants is mainly caused by H2Discharge of S causes H2S and sulfur-containing compounds can account for 80-90% of the malodorous compounds, and the concentration of hydrogen sulfide can be as high as 0.145 mg/L.
The source deodorization technology of the sewage treatment plant is a relatively ideal stink control technology. In addition, in the sewage treatment process, in order to fully play the role of microorganisms in the activated sludge, part of sludge-water mixture in the biochemical treatment process can be refluxed to the front end for recycling, and the refluxed activated sludge generally has the potential of biologically oxidizing most of compounds causing odor disturbance, but the types of efficient desulfurization bacteria discovered from the refluxed activated sludge at present are few, and the realization of large-scale application of the deodorization technology at the source of the refluxed sludge is far away.
Disclosure of Invention
The invention aims to provide a Klebsiella and application thereof, which can remove odor generated in the sewage treatment process at the source.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a Klebsiella pneumoniae which has a preservation name of Klebsiella pneumoniae HZ1, a Latin is Klebsiella pneumoniae, and the preservation date is as follows: 16/9/2021, depository: china general microbiological culture Collection center, preservation number: CGMCC No. 23424.
Preferably, the sequencing result of the 16S rDNA of the Klebsiella pneumoniae is shown as SEQ ID NO. 1.
The invention also provides application of the Klebsiella pneumoniae in sewage desulfurization and deodorization.
Preferably, the klebsiella pneumoniae is used by inoculating a klebsiella pneumoniae bacterial solution into sewage.
Preferably, the inoculation ratio of the Klebsiella pneumoniae bacterial liquid at the time of inoculation is 4-13% (v/v%).
Preferably, the bacterial density of the Klebsiella pneumoniae bacterial liquid is 2-4 × 108one/mL.
Preferably, the temperature of the Klebsiella pneumoniae is 15-30 ℃ and the pH value is 6-8 in the using process.
Preferably, the concentration of sulfur ions in the sewage is 800-1000 mg/L.
The invention provides a Klebsiella and application thereof, wherein the preservation name of the Klebsiella is Klebsiella pneumoniae HZ1, the preservation date of the Klebsiella pneumoniae is as follows: 16/9/2021, depository: china general microbiological culture Collection center, preservation number: CGMCC No. 23424. When 800mg/L sodium sulfide solution is treated by the Klebsiella pneumoniae of the invention, the removal rate of sulfur ions reaches 89.27 percent, and malodorous substances generated in the sewage treatment process can be greatly reduced. The desulfurization conditions required by the strain are easy to realize and control, and the strain has strong desulfurization capability and good application prospect. Not only can efficiently deodorize, but also can reduce the odor treatment cost, and has positive significance for the construction and development of sewage treatment plants in cities.
Deposit description
Klebsiella pneumoniae HZ1, Klebsiella pneumoniae, which is deposited in the China general microbiological culture Collection center (CGMCC) with the addresses of: the preservation date is 2021 year, 9 month and 16 days, and the preservation numbers are: CGMCC No. 23424.
Drawings
FIG. 1 shows the results of desulfurization rates of examples 1 to 3 and comparative examples 1 to 6.
Detailed Description
The invention provides a Klebsiella pneumoniae which has a preservation name of Klebsiella pneumoniae HZ1, a Latin is Klebsiella pneumoniae, and the preservation date is as follows: 16/9/2021, depository: china general microbiological culture Collection center, preservation number: CGMCC No. 23424.
In the invention, the sequencing result of the 16S rDNA of the Klebsiella pneumoniae is shown in SEQ ID No. 1.
The invention also provides application of the Klebsiella pneumoniae in sewage desulfurization and deodorization.
In the present invention, it is preferable that the Klebsiella pneumoniae is used by inoculating a Klebsiella pneumoniae bacterial solution into sewage.
In the present invention, the inoculation ratio of the Klebsiella pneumoniae bacterial liquid at the time of inoculation is preferably 4 to 13% (v/v%), more preferably 7 to 10% (v/v%), and still more preferably 8 to 9% (v/v%).
In the invention, the bacterial density of the Klebsiella pneumoniae bacterial liquid is preferably 2-4 × 108one/mL, more preferably 3X 108one/mL.
In the invention, the temperature of the Klebsiella pneumoniae is preferably 15-30 ℃ in the using process, and is further preferably 20-25 ℃.
In the invention, the pH value of the Klebsiella pneumoniae is preferably 6-8, more preferably 6.5-7.5, and even more preferably 7.33 in the using process.
In the invention, the concentration of sulfur ions in the sewage is preferably 800-1000 mg/L, more preferably 850-950 mg/L, and still more preferably 900 mg/L.
In the invention, the using time of the Klebsiella pneumoniae in the using process is preferably 100-140 min, more preferably 110-130 min, and still more preferably 120 min.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
The materials used in the examples are as follows:
1. enrichment culture medium: glucose 5.0g/L, Na2S·9H2O 5g/L；KH2PO41.0 g/L、 MgCl2·6H2O 0.8g/L、K2HPO41.0 g/L、NH4Cl 0.4g/L、FeSO4·7H2O0.01 g/L, using water as solvent, adjusting pH to 7.33 before use, and sterilizing at 121 deg.C for 30 min.
2. Preparing bacterial liquid: the Klebsiella pneumoniae HZ1 is inoculated into a 250 mL conical flask filled with 100mL enrichment medium, and is placed in a shaking table for culturing at 30 ℃ and 160r/min for 5d by shaking.
Example 1
Taking 5mL of bacterial liquid subjected to shake culture in an enrichment medium for 3d, placing the bacterial liquid in a constant-temperature magnetic stirrer with 100mL of sodium sulfide solution with the concentration of 800mg/L, adding a rotor into the mixed liquid, stirring the mixed liquid on the constant-temperature magnetic stirrer, adjusting the temperature of the solution to be 30 ℃ and the rotating speed to be 1000r/min, sampling and detecting the mass of sulfur ions in the solution after 120min, and calculating the desulfurization rate.
Comparative example 1
The procedure was as in example 1 except that the temperature of the solution was adjusted to 15 ℃.
Comparative example 2
The procedure was the same as in example 1 except that the temperature of the solution was adjusted to 25 ℃.
Example 2
Taking 5mL of bacterial liquid subjected to shake culture in an enrichment medium for 3d, placing the bacterial liquid in a sulfide solution with the concentration of 100mL being 800mg/L, adding a rotor into the mixed liquid, placing the mixed liquid on a constant-temperature magnetic stirrer for stirring, adjusting the temperature of the solution to 35 ℃, adjusting the pH to 7.33 and the rotating speed to 1000r/min, sampling after 120min, detecting the mass of sulfur ions in the solution, and calculating the desulfurization rate.
Comparative example 3
The procedure was as in example 2 except that the pH of the solution was adjusted to 6.56.
Comparative example 4
The procedure was as in example 2 except that the pH of the solution was adjusted to 8.09.
Example 3
Taking 15mL of bacterial liquid subjected to shake culture in an enrichment medium for 3d, placing the bacterial liquid in a sulfide solution with the concentration of 100mL and the concentration of 800mg/L (the inoculation ratio is 13%), adding a rotor into the mixed solution, placing the mixed solution on a constant-temperature magnetic stirrer for stirring, adjusting the temperature of the solution to 35 ℃, the pH to 7.33 and the rotating speed to 1000r/min, sampling after 120min, detecting the mass of sulfur ions in the solution, and calculating the desulfurization rate.
Comparative example 5
The procedure was as in example 3 except that the inoculum size of the shake culture 3d in the enrichment medium was 5mL (inoculum ratio was 5%).
Comparative example 6
The procedure was the same as in example 3 except that the inoculum size of the shake culture 3d in the enrichment medium was 10mL (9% inoculum size ratio).
And (4) conclusion: the results are shown in Table 1.
TABLE 1
The results showed that in example 1, the desulfurization rate was 80.47% at a temperature of 30 ℃, whereas in comparative example 1 and comparative example 2, i.e., at temperatures of 15 ℃ and 25 ℃, respectively, the desulfurization rate was slightly decreased to 74.67% and 77.57%, respectively. In example 2, the desulfurization rate was 82.18% in the case of pH 7.33, i.e., near neutrality, while in comparative examples 3 and 4, i.e., pH 6.56 and 8.09, respectively, the desulfurization rate was slightly decreased to 77.18% and 79.32%, respectively. In example 3, the desulfurization rate was 89.27% when the inoculum size was 15mL, i.e., the inoculum ratio was 13% (v/v%), whereas in comparative examples 5 and 6, i.e., the inoculum ratios were 5% and 9% (v/v%), respectively, the desulfurization rates were slightly decreased, i.e., 86.19% and 79.27%, respectively, and the statistical results are shown in FIG. 1.
As described above, when the temperature was 30 ℃ and the pH was about 7 (i.e., neutral conditions) and the inoculum size was 13% (v/v%), the desulfurization effect of the desulfurization bacteria was the best, and the desulfurization rate was 89.27% at the highest.
From the above examples, the present invention provides a strain of klebsiella and its application, wherein the klebsiella deposit is designated as klebsiella pneumoniae HZ1, the latin is klebsiella pneumoniae, the deposit date is: 16/9/2021, depository: china general microbiological culture Collection center, preservation number: CGMCC No. 23424. When 800mg/L sodium sulfide solution is treated by the Klebsiella pneumoniae of the invention, the removal rate of sulfur ions reaches 89.27 percent, and malodorous substances generated in the sewage treatment process can be greatly reduced. The desulfurization conditions required by the strain are easy to realize and control, and the strain has strong desulfurization capability and good application prospect. Not only can efficiently deodorize, but also can reduce the odor treatment cost, and has positive significance for the construction and development of sewage treatment plants in cities.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Sequence listing
<110> Beijing university of forestry
West Yuan environmental engineering (Shanghai) Co Ltd
Klebsiella and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1404
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 1
gtagcgccct cccgaaggtt aagctaccta cttcttttgc acccactccc atggtgtgac 60
gggcggtgtg tacaaggccc gggaacgtat tcaccgtagc attctgatct acgattacta 120
gcgattccga cttcatggag tcgagttgca gactccaatc cggactacga catactttat 180
gaggtccgct tgctctcgcg aggtcgcttc tctttgtata tgccattgta gcacgtgtgt 240
agccctggtc gtaagggcca tgatgacttg acgtcatccc caccttcctc cagtttatca 300
ctggcagtct cctttgagtt cccggcctaa ccgctggcaa caaaggataa gggttgcgct 360
cgttgcggga cttaacccaa catttcacaa cacgagctga cgacagccat gcagcacctg 420
tctcacagtt cccgaaggca ccaaagcatc tctgctaagt tctgtggatg tcaagaccag 480
gtaaggttct tcgcgttgca tcgaattaaa ccacatgctc caccgcttgt gcgggccccc 540
gtcaattcat ttgagtttta accttgcggc cgtactcccc aggcggtcga tttaacgcgt 600
tagctccgga agccacgcct caagggcaca acctccaaat cgacatcgtt tacggcgtgg 660
actaccaggg tatctaatcc tgtttgctcc ccacgctttc gcacctgagc gtcagtcttt 720
gtccaggggg ccgccttcgc caccggtatt cctccagatc tctacgcatt tcaccgctac 780
acctggaatt ctacccccct ctacaagact ctagcctgcc agtttcgaat gcagttccca 840
ggttgagccc ggggatttca catccgactt gacagaccgc ctgcgtgcgc tttacgccca 900
gtaattccga ttaacgcttg caccctccgt attaccgcgg ctgctggcac ggagttagcc 960
ggtgcttctt ctgcgggtaa cgtcaatcga caaggttatt aaccttatcg ccttcctccc 1020
cgctgaaagt gctttacaac ccgaaggcct tcttcacaca cgcggcatgg ctgcatcagg 1080
cttgcgccca ttgtgcaata ttccccactg ctgcctcccg taggagtctg gaccgtgtct 1140
cagttccagt gtggctggtc atcctctcag accagctagg gatcgtcgcc taggtgagcc 1200
gttaccccac ctactagcta atcccatctg ggcacatctg atggcatgag gcccgaaggt 1260
cccccacttt ggtcttgcga catnatgcgg tattagctac cgtttccagt agttatcccc 1320
ctccatcagg cagtttccca gacattactc acccgtccgc cgctcgtcac ccgagagcaa 1380
gctctctgtg ctaccgctcg actg 1404
Claims (8)
1. The Klebsiella pneumoniae is characterized in that the preservation name of the Klebsiella pneumoniae is Klebsiella pneumoniae HZ1, the Latin is Klebsiella pneumoniae, and the preservation date is as follows: 16/9/2021, depository: china general microbiological culture Collection center, preservation number: CGMCC No. 23424.
2. The Klebsiella pneumoniae of claim 1, wherein the sequence of the 16S rDNA of the Klebsiella pneumoniae is shown in SEQ ID No. 1.
3. Use of the Klebsiella pneumoniae of claim 1 or 2 in desulfurization and deodorization of wastewater.
4. The use according to claim 3, wherein the Klebsiella pneumoniae is used by inoculating a Klebsiella pneumoniae bacterial solution into sewage.
5. The use according to claim 4, wherein the inoculation ratio of the Klebsiella pneumoniae bacterial liquid at the time of inoculation is 4-13% (v/v%).
6. The use according to claim 5, wherein the bacterial density of the Klebsiella pneumoniae bacterial liquid is 2-4 x 108one/mL.
7. The use of claim 6, wherein the temperature of the Klebsiella pneumoniae is 15-30 ℃ and the pH is 6-8 during the use process.
8. The use of claim 7, wherein the concentration of sulfur ions in the wastewater is 800-1000 mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111455855.XA CN114149946B (en) | 2021-12-01 | 2021-12-01 | Klebsiella and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111455855.XA CN114149946B (en) | 2021-12-01 | 2021-12-01 | Klebsiella and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114149946A true CN114149946A (en) | 2022-03-08 |
| CN114149946B CN114149946B (en) | 2024-01-16 |
Family
ID=80455672
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111455855.XA Active CN114149946B (en) | 2021-12-01 | 2021-12-01 | Klebsiella and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114149946B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102409012A (en) * | 2011-10-19 | 2012-04-11 | 黑龙江大学 | Klebsiella pneumonia being separated from Termite gut |
| CN109055282A (en) * | 2018-09-27 | 2018-12-21 | 陕西科技大学 | One Klebsiella pneumoniae new strains and its separation method and application |
-
2021
- 2021-12-01 CN CN202111455855.XA patent/CN114149946B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102409012A (en) * | 2011-10-19 | 2012-04-11 | 黑龙江大学 | Klebsiella pneumonia being separated from Termite gut |
| CN109055282A (en) * | 2018-09-27 | 2018-12-21 | 陕西科技大学 | One Klebsiella pneumoniae new strains and its separation method and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114149946B (en) | 2024-01-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110117567B (en) | Paracoccus denitrificans strain screening and application thereof in deodorization | |
| CN110697884B (en) | Method for culturing bacteria-algae symbiotic granular sludge at low temperature | |
| CN102443550B (en) | Screening method of denitrifying bacteria | |
| CN113736717B (en) | Methane oxidizing bacterium with denitrification function and anoxia resistance and application thereof | |
| WO2021077453A1 (en) | Pseudomonas stutzeri, composite microbial inoculum prepared using the pseudomonas stutzeri, and use of the composite microbial inoculum | |
| CN105647838B (en) | Skin spy's acinetobacter calcoaceticus and application thereof | |
| CN108546648A (en) | A kind of environment-friendly type microalgae culture method | |
| CN109161499A (en) | A kind of application for producing surfactant bacterium and its cutting down polycyclic aromatic hydrocarbon in situ in coal/petrochemical wastewater | |
| CN109749958B (en) | Aerobic denitrifying phosphorus accumulating bacterium for nitrogen and phosphorus removal in high-alkaline environment and application thereof | |
| CN101811779B (en) | Preparation method of halophilic decontamination bacterial agent and bacterial agent prepared by same | |
| CN107118982B (en) | Thiobacillus ferrooxidans and application thereof | |
| CN102690765A (en) | Low-temperature aerobic denitrifying strain Pseudomonas psychrophila Den-03 and screening method and application thereof | |
| CN110438025A (en) | A kind of denitrogenation bacterial strain and its application | |
| CN106927576A (en) | A kind of method of nitrogen pollutant removal effect in raising sewage | |
| CN109055259B (en) | Pseudomonas XD-3, application thereof and microbial flocculant | |
| CN104480045A (en) | Efficient aerobic denitrifying strain having heterotrophic nitrification function and application of strain | |
| CN114149946A (en) | Klebsiella and application thereof | |
| CN117143755A (en) | Pseudomonas stutzeri strain and application thereof | |
| CN111548952B (en) | Method for domesticating microbial flora for degrading efficient sulfur-series malodorous substances | |
| CN114874938A (en) | Bacillus cereus S5 for degrading hydrogen sulfide gas and application thereof | |
| CN210163450U (en) | Special equipment for carrying out optimal expanding culture on sludge protobacteria group to treat sewage | |
| CN112694987A (en) | Method for enriching and domesticating perchlorate reducing flora by using conductive material | |
| CN109055225B (en) | Enrichment culture method of methane anaerobic oxidizing bacteria flora | |
| CN111268871B (en) | Shrimp breeds sewage purification unit | |
| Du et al. | Identification of a highly efficient aerobic denitrifying bacterium in SBR and denitrification optimization |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Hong Yu Inventor after: Li Lihua Inventor after: Zhang Haoge Inventor after: Ju Qingling Inventor after: Cao Xiaoxin Inventor before: Hong Yu Inventor before: Li Lihua Inventor before: Zhang Haoge Inventor before: Ju Qingling Inventor before: Cao Xiaoxin |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 100083 No. 35 Qinghua East Road, Beijing, Haidian District Patentee after: BEIJING FORESTRY University Country or region after: China Patentee after: Xiyuan environmental protection (Shanghai) Co.,Ltd. Address before: 100083 No. 35 Qinghua East Road, Beijing, Haidian District Patentee before: BEIJING FORESTRY University Country or region before: China Patentee before: NISHIHARA ENVIRONMENT ENGINEERING (SHANGHAI) Co.,Ltd. |
|
| CP03 | Change of name, title or address |