CN114145390A - Preparation method of traditional Chinese medicine fermented feed additive for strengthening nutrition and treating heat stress - Google Patents
Preparation method of traditional Chinese medicine fermented feed additive for strengthening nutrition and treating heat stress Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a preparation method of a traditional Chinese medicine fermented feed additive for strong nutrition and heat stress treatment, belonging to the technical field of bioengineering; the method comprises the following steps: (1) mixing powder; (2) primary fermentation; (3) performing secondary fermentation; (3) carrying out tertiary fermentation; (4) fermenting in a breathing bag. The traditional Chinese medicine fermented feed additive prepared by the method has high lysine content, and can improve the nutrition supply, reduce the heat stress reaction and improve the milk yield of the dairy cow in high-temperature seasons when being added into the dairy cow feed.
Description
Technical Field
The invention belongs to the technical field of bioengineering, and particularly relates to a preparation method of a traditional Chinese medicine fermented feed additive for treating heat stress through strong nutrition.
Background
The Chinese herbal medicine as the feed additive or the mixed feed medicament is an important aspect of veterinary Chinese herbal medicine application in recent years and is a unique series of feed additives. Because the traditional Chinese medicine is purely natural, has rich resources, easy acquisition and small toxic and side effects, has no harmful residues in animal bodies and meat, eggs and milk, and can create green and safe food for people.
In the process of raising dairy cows, in summer with high temperature, the dairy cows induce mastitis due to heat stress and the like, resulting in reduction of milk yield and milk quality. Aiming at the breeding of the dairy cows, a nontoxic and safe traditional Chinese medicine fermented feed additive is developed, the heat stress reaction of the dairy cows is relieved, the yield of milk is improved, and the method has important significance for the breeding of the ruminant dairy cows.
Disclosure of Invention
The invention discloses a preparation method of a traditional Chinese medicine fermented feed additive for treating heat stress by strong nutrition.
In order to achieve the purpose, the invention adopts the following technical scheme:
a preparation method of a traditional Chinese medicine fermented feed additive for strengthening nutrition and treating heat stress comprises the following steps:
(1) powder mixing:
mixing flos Sophorae Immaturus powder, bentonite, vermiculite powder, rare earth, soybean meal powder and glucose to obtain a mixture A;
mixing radix Codonopsis powder, radix Glycyrrhizae Preparata powder, radix astragali powder, Atractylodis rhizoma powder, fructus Gardeniae powder, and herba Epimedii powder to obtain mixture B;
(2) primary fermentation:
inoculating lactobacillus acidophilus liquid and bacillus subtilis liquid to the mixture A, adding cellulase, ligninase and water, and performing static fermentation to obtain primary fermentation mash AA;
(3) and (3) secondary fermentation:
mixing the mixture A with the mixture B to obtain a mixture AA 2;
mixing the mixture AA2 with the primary fermented liquor AA, inoculating lactobacillus plantarum bacterial liquid, clostridium butyricum bacterial liquid and yeast bacterial liquid, and performing static fermentation to obtain a secondary fermentation substrate BB;
(3) and (3) fermenting for the third time:
adding vermiculite powder, bentonite, peptone and glucose into yeast liquid, culturing overnight, adding radix astragali powder, and continuously culturing to obtain secondary yeast culture solution;
mixing the mixture B with a secondary fermentation substrate BB, adding a lignin enzyme and yeast secondary culture solution, and performing static fermentation to obtain a fermentation substrate CC;
(4) fermentation in a breathing bag:
and (3) filling the fermentation substrate CC into a breathing bag, sealing the breathing bag, and standing for fermentation to obtain the traditional Chinese medicine fermented feed additive.
In the raw materials used in the invention, the radix codonopsitis powder can improve the immunity, the honey-fried licorice root powder plays a role in blending, the astragalus powder invigorates qi, the bighead atractylodes rhizome powder tonifies the stomach and the spleen, the gardenia powder clears heat and promotes diuresis, cools blood and detoxifies, and the epimedium powder mainly repairs breast systems; all the raw materials are matched with each other, so that the digestion capacity is further improved, the immunity is enhanced, and the resistance to heat stress is improved; meanwhile, flos sophorae can effectively reduce heat stress reaction by clearing heat and soothing nerves and matching with cape jasmine to clear heat and promote diuresis. In addition, the lysine content can be increased, the nutrient absorption and nutrient balance can be improved, the resistance to heat stress can be improved, the immunity can be improved, the reproductive system can be repaired, and the milk yield can be improved after the raw materials are mixed and fermented and subjected to enzymolysis. Oxygen in the material layer is consumed by the aerobic bacteria bacillus subtilis, a proper environment is provided for anaerobic metabolism of saccharomycetes and metabolism of lactobacillus acidophilus, generation of beneficial metabolites is further promoted, and the resistance to heat stress is enhanced while nutrition absorption is enhanced. The bentonite, the vermiculite powder and the rare earth have the regulation and control effects on the metabolism of strains, the promotion effect on the activity of enzymes and the regulation and control effects on the metabolism of the cow organism.
In conclusion, the invention forms a synergistic organic system through traditional Chinese medicines, bacteria, enzymes, trace elements and the like, improves the lysine content, promotes the nutrient digestion and absorption, enhances the efficacy of the traditional Chinese medicines, improves the immunity, prevents and treats mastitis, thereby enhancing the resistance to heat stress and improving the milk yield.
Preferably, in the step (1),
the mass ratio of the sophora flower powder, the bentonite, the vermiculite powder, the rare earth, the soybean meal powder and the glucose is 8: 1: 1: 1: 2: 1;
the mass ratio of the radix codonopsitis powder, the roasted liquorice powder, the astragalus powder, the white atractylodes rhizome powder, the gardenia powder and the epimedium powder is 0.2: 3: 1: 1.5: 2: 0.2.
preferably, in the step (2),
the static fermentation procedure was:
sealing the fermentation container with gauze, and fermenting at 37 deg.C for 36 hr;
removing gauze, sealing with a sealing cover, and fermenting at 37 deg.C for 48 hr;
removing the sealing cover, adding cellulase and ligninase, sealing with gauze, and fermenting at 28 deg.C for 36 hr.
Preferably, in the step (2),
before static fermentation, adding 20L of lactobacillus acidophilus liquid, 20L of bacillus subtilis liquid, 1kg of cellulase, 2kg of ligninase and 200kg of water into every 30kg of mixture A;
after the sealing cover is removed, 1kg of cellulase and 2kg of ligninase are added into every 30kg of the mixture A.
Preferably, in the step (3),
fermenting in a sterile room for 36h at 35 ℃ without covering the fermentation container.
Preferably, in the step (3),
mixing the mixture A and the mixture B according to the mass ratio of 1:4 to obtain a mixture AA 2;
the mixture AA2 and the primary fermented mash AA are mixed according to the weight ratio of 1.5: 1-2.5: 1, mixing in a mass ratio; and 5L of lactobacillus plantarum bacterial liquid, 3L of clostridium butyricum bacterial liquid and 10L of yeast bacterial liquid are added into each 600kg of the mixture AA 2.
Preferably, in the step (4),
adding 0.2% by mass of vermiculite powder, 0.1% by mass of bentonite, 1% by mass of peptone and 1% by mass of glucose into yeast liquid, sealing a fermentation container with gauze, culturing for 16h at 28 ℃, adding 0.2% by mass of radix astragali powder, and continuously culturing for 8h to obtain a secondary yeast culture solution;
the mixture B and a secondary fermentation substrate BB are mixed according to the ratio of 1: 5-1: 7, mixing according to a mass ratio; adding 3kg of ligninase, 15L of yeast secondary culture solution and 48kg of water into every 150kg of the mixture B; fermenting the fermentation container without covering in a sterile room at 28 deg.C for 6h, and then heating to 38 deg.C for 8 h.
Preferably, in step (5),
the static fermentation conditions were: fermenting at 30 deg.C for 2 days.
Preferably, the Lactobacillus acidophilus liquid is prepared by culturing Lactobacillus acidophilus at 33 deg.C for 22h at 180 r/min;
the Bacillus subtilis liquid is prepared by culturing Bacillus subtilis at 33 deg.C for 18h at 180 r/min;
the lactobacillus plantarum solution is prepared by culturing lactobacillus plantarum at 33 ℃ for 18h at 180 r/min;
the clostridium butyricum bacterial liquid is prepared by culturing clostridium butyricum at 33 ℃ for 22 hours at 180 r/min;
the yeast liquid is prepared by culturing yeast at 33 deg.C for 18h at 180 r/min.
Preferably, the components of the culture medium used for preparing the bacterial liquid of each bacterium are as follows: 1 percent of yeast extract, 1 percent of peptone and 4 percent of glucose.
In conclusion, the traditional Chinese medicine fermented feed additive prepared by the invention has high lysine content, and can improve the nutrition supply, reduce the heat stress reaction and improve the milk yield of the dairy cow in high-temperature seasons when being added into the dairy cow feed.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the examples, bentonite was purchased from Shijiazhuang Guangning minerals, Inc.; vermiculite was purchased from mingbo mineral products ltd, lingshou county; radix Codonopsis and radix Glycyrrhizae Preparata are obtained from Hebei Baicao Kangsheng pharmaceutical Co Ltd; radix astragali is purchased from radix astragali industry Co., Ltd in Shanxi Dongdao; atractylodis rhizoma is obtained from Cistanchis herba of Tiantai Gaoshan Atractylodis rhizoma; gardenia is purchased from medicinal material general company of Bozhou city, Anhui province; the soybean meal is purchased from Shandong Fuliheng New Material science and technology Co., Ltd; epimedium was purchased from Bailintang pharmaceutical Co., Ltd, Bozhou city; flos sophorae was purchased from bai-26384m county hua diligent fodder limited; cellulase CEL-01, type CEL, was purchased from the summer prosperous group; ligninase 1000U technical grade was purchased from Shandonogojie Biotech Ltd; the rare earth is purchased from Zhengde rare earth materials, Inc. of Suzhou city; lactobacillus acidophilus was purchased from galyi, chinese; saccharomyces cerevisiae and Bacillus subtilis were purchased from Cangzhou Wang Producer technology research institute, Inc.; clostridium butyricum was purchased from new yangshao biotechnology limited of hannan; lactobacillus plantarum was purchased from Weifang Rui biotechnology Limited.
Example 1
A method for preparing a Chinese medicinal fermented feed additive for nourishing and treating heat stress comprises:
1. pulverizing
Pulverizing vermiculite, radix Codonopsis, radix Glycyrrhizae Preparata, radix astragali, Atractylodis rhizoma, fructus Gardeniae, soybean meal, herba Epimedii, and flos Sophorae with water drop type pulverizer, and sieving with 80 mesh sieve to obtain radix Codonopsis powder, radix Glycyrrhizae Preparata powder, radix astragali powder, Atractylodis rhizoma powder, fructus Gardeniae powder, soybean meal powder, herba Epimedii powder, flos Sophorae powder, and vermiculite powder.
2. Preparation of bacterial liquid
Strain activation medium: 1% (w/w) of yeast extract, 1% (w/w) of peptone and 4% (w/w) of glucose.
Preparing bacterial liquid: filling 300mL of activation medium into a 500mL triangular flask, inoculating strains, and culturing at 33 ℃ at a rotating speed of 180 r/m; culturing the saccharomycetes for 18h to obtain a saccharomycete liquid; culturing lactobacillus plantarum and bacillus subtilis for 18h, and culturing lactobacillus acidophilus and clostridium butyricum for 22h to respectively obtain lactobacillus plantarum liquid, bacillus subtilis liquid, lactobacillus acidophilus liquid and clostridium butyricum liquid. The triangular bottle for lactobacillus plantarum, lactobacillus acidophilus and clostridium butyricum is wrapped at the bottle mouth by a multilayer preservative film, and then the preservative film is wound by a transparent adhesive tape to ensure that the air leakage or air intake is avoided.
3. Mixing of powder material
According to the method, the method comprises the following steps of: bentonite: vermiculite powder: rare earth: soybean meal powder: the weight ratio of glucose is 8: 1: 1: 1: 2: 1, uniformly mixing to obtain a mixture A.
According to the ratio of radix codonopsitis powder: roasted licorice powder: radix astragali powder: white atractylodes rhizome powder: gardenia powder: the weight ratio of the epimedium powder is 0.2: 3: 1: 1.5: 2: 0.2, and uniformly mixing to obtain a mixture B.
4. Primary fermentation
Adding 20L of lactobacillus acidophilus liquid, 20L of bacillus subtilis liquid, 1kg of cellulase, 2kg of ligninase and 200kg of sterilized distilled water into 30kg of the mixture A, putting the mixture A into 200L plastic barrels, putting 130-layer sand-adding 160L of each plastic barrel, sealing the six layers of gauze, and transferring the mixture A into 2 barrels.
Standing at 37 deg.C for 36 h; removing gauze, covering with a sealing cover, and standing at 37 deg.C for fermentation for 48 hr; removing the sealing cover, adding 1kg of cellulase, 2kg of ligninase and six layers of gauze, sealing, and standing and fermenting at 28 ℃ for 36 hours to obtain primary fermentation mash AA.
5. Secondary fermentation
And (3) mixing 120kg of the mixture B with 480kg of the mixture A (the proportion of 1: 4) to obtain a mixture AA2, mixing the mixture AA2 (600 kg) with the primary fermented mash AA, adding 5L of lactobacillus-implanted bacterial liquid, 3L of clostridium butyricum bacterial liquid and 10L of yeast bacterial liquid, and uniformly mixing to obtain a secondary fermented material.
Taking an uncovered stainless steel tank (3mx1mx2m) as a fermentation tank, putting all the secondary fermentation materials into the fermentation tank, putting the fermentation tank into a sterile room, and standing and fermenting for 36h at 35 ℃ to obtain a secondary fermentation substrate BB.
6. Three times of fermentation
Adding 0.2 mass percent of vermiculite powder and 0.1 mass percent of bentonite into 15L of yeast liquid, adding 1 mass percent of peptone and 1 mass percent of glucose, putting into a 200L plastic barrel, sealing with 6 layers of gauze, culturing at 28 ℃ for 16h, then adding 0.2 mass percent of astragalus powder, and continuously culturing for 8h to obtain the secondary yeast culture solution.
And taking 150kg of the mixture B, mixing the mixture B with a secondary fermentation substrate BB, adding 3kg of ligninase and 15L of yeast secondary culture solution, adding 48kg of sterilized tap water, uniformly mixing, filling the mixture into a stainless steel fermentation tank, putting the stainless steel fermentation tank into a sterile room, culturing for 6 hours at the temperature of 28 ℃, then heating to 38 ℃, culturing for 8 hours, and obtaining a fermentation substrate CC after the fermentation is finished.
7. Fermentation of breath bags
The fermentation substrate CC was placed in breathing bags (feed fermentation breathing bags, 25kg, 51X 81X 10 filaments, Wenzhou Chuangjia packaging materials Co., Ltd.), each bag was packed with 15kg, and heat-sealed with a plastic sealing machine (plastic bag sealing machine, Xiangbo electromechanical device Co., Ltd., Dongguan city). Fermenting at 30 deg.C for 2 days to obtain Chinese medicinal fermented feed additive.
8. Quality analysis
Carrying out acidolysis on a sample according to GB/T18246-2000, and determining the content of lysine in the traditional Chinese medicine fermented feed additive by an indetrione method; the lysine content of the traditional Chinese medicine fermented feed additive is 0.48% by calculating the value of the lysine content by taking the sample water content as 10%.
Selecting 24 healthy black-white cattle aged 4-5 years, with similar weight, gestation, lactation period and breast somatic cells, randomly dividing into two groups: control and experimental groups. And (3) starting the experiment in the Hubei experiment base in the middle 6 th of the month, and calculating the feed intake according to the difference between fed materials and residual materials after feeding every day. The control group is not added with the traditional Chinese medicine fermented feed additive, the experimental group is added with 0.2 percent of the traditional Chinese medicine fermented feed additive according to the feed quality, and the experimental period is 56 days. The effect of the fermented traditional Chinese medicine additive on the daily milk production performance of the dairy cows is shown in table 1.
TABLE 1
Comparative example 1
In the whole production process, no ligninase is added, and in other same examples 1, the lysine content in the traditional Chinese medicine fermented feed additive is measured to be 0.39%, the traditional Chinese medicine fermented feed additive is applied to feed addition according to the method in the example 1, and the influence of the fermented traditional Chinese medicine additive on the daily milk production performance of the dairy cow is shown in table 2.
TABLE 2
Comparative example 2
30kg of the mixture A is added with 20L of lactobacillus acidophilus liquid, 20L of bacillus subtilis liquid, 2kg of cellulase, 4kg of ligninase and 200kg of sterilized distilled water, and the mixture A is directly and uniformly mixed with the mixture AA2600kg without primary fermentation for fermentation, the fermentation conditions are completely the same as the secondary fermentation conditions of the embodiment 1, the subsequent steps are the same as the embodiment 1, the lysine content in the traditional Chinese medicine fermented feed additive is measured to be 0.31 percent, the traditional Chinese medicine fermented feed additive is applied to feed addition according to the method of the embodiment 1, and the influence of the fermented traditional Chinese medicine additive on the daily milk production performance of the dairy cow is shown in Table 3.
TABLE 3
Comparative example 2
Except for the primary fermentation without adding lactobacillus acidophilus liquid, the content of lysine in the traditional Chinese medicine fermented feed additive is measured to be 0.46% in the same example 1, the traditional Chinese medicine fermented feed additive is applied to feed addition according to the method of the example 1, and the influence of the fermented traditional Chinese medicine additive on the daily milk production performance of the dairy cow is shown in table 4.
TABLE 4
Comparative example 3
Except that 0.2% of vermiculite powder and 0.1% of bentonite are not added into the yeast liquid in the third fermentation, the lysine content in the traditional Chinese medicine fermented feed additive is measured to be 0.41% in the same example 1, the traditional Chinese medicine fermented feed additive is applied to feed addition according to the method of the example 1, and the influence of the fermented traditional Chinese medicine additive on the daily milk production performance of the dairy cow is shown in table 5.
TABLE 5
Comparative example 4
The same as example 1 except that the third fermentation was not performed, the substrate BB was fermented twice, 15L of yeast liquid (containing 0.2% of vermiculite powder and 0.1% of bentonite, 1% of peptone and 1% of glucose, and 0.2% of Astragalus powder) and 150kg of the mixture B were directly added, mixed uniformly, and the respiratory bag fermentation was performed directly, the lysine content in the fermented feed additive was found to be 0.37%, and the fermented feed additive was added to the feed according to the method of example 1, and the effect of the fermented feed additive on the daily milk production performance of the cow is shown in Table 6.
TABLE 6
Comparative example 5
The same procedure as in example 1 was repeated except that the sophora japonica was not added and replaced with the same amount of soybean meal, and the lysine content was measured to be 1.3%, and the fermented traditional Chinese medicine additive was added to the feed according to the method in example 1, and the effect of the fermented traditional Chinese medicine additive on the daily milk production performance of the cow is shown in table 7.
TABLE 7
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to the above-described embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A preparation method of a traditional Chinese medicine fermented feed additive for strengthening nutrition and treating heat stress is characterized by comprising the following steps:
(1) powder mixing:
mixing flos Sophorae Immaturus powder, bentonite, vermiculite powder, rare earth, soybean meal powder and glucose to obtain a mixture A;
mixing radix Codonopsis powder, radix Glycyrrhizae Preparata powder, radix astragali powder, Atractylodis rhizoma powder, fructus Gardeniae powder, and herba Epimedii powder to obtain mixture B;
(2) primary fermentation:
inoculating lactobacillus acidophilus liquid and bacillus subtilis liquid to the mixture A, adding cellulase, ligninase and water, and performing static fermentation to obtain primary fermentation mash AA;
(3) and (3) secondary fermentation:
mixing the mixture A with the mixture B to obtain a mixture AA 2;
mixing the mixture AA2 with the primary fermented liquor AA, inoculating lactobacillus plantarum bacterial liquid, clostridium butyricum bacterial liquid and yeast bacterial liquid, and performing static fermentation to obtain a secondary fermentation substrate BB;
(3) and (3) fermenting for the third time:
adding vermiculite powder, bentonite, peptone and glucose into yeast liquid, culturing overnight, adding radix astragali powder, and continuously culturing to obtain secondary yeast culture solution;
mixing the mixture B with a secondary fermentation substrate BB, adding a lignin enzyme and yeast secondary culture solution, and performing static fermentation to obtain a fermentation substrate CC;
(4) fermentation in a breathing bag:
and (3) filling the fermentation substrate CC into a breathing bag, sealing the breathing bag, and standing for fermentation to obtain the traditional Chinese medicine fermented feed additive.
2. The preparation method of the Chinese medicinal fermented feed additive for enhancing nutrition and treating heat stress according to claim 1,
in the step (1), the step (c),
the mass ratio of the sophora flower powder, the bentonite, the vermiculite powder, the rare earth, the soybean meal powder and the glucose is 8: 1: 1: 1: 2: 1;
the mass ratio of the radix codonopsitis powder, the roasted liquorice powder, the astragalus powder, the white atractylodes rhizome powder, the gardenia powder and the epimedium powder is 0.2: 3: 1: 1.5: 2: 0.2.
3. the preparation method of the Chinese medicinal fermented feed additive for enhancing nutrition and treating heat stress according to claim 1,
in the step (2),
the static fermentation procedure was:
sealing the fermentation container with gauze, and fermenting at 37 deg.C for 36 hr;
removing gauze, sealing with a sealing cover, and fermenting at 37 deg.C for 48 hr;
removing the sealing cover, adding cellulase and ligninase, sealing with gauze, and fermenting at 28 deg.C for 36 hr.
4. The preparation method of the Chinese medicinal fermented feed additive for enhancing nutrition and treating heat stress according to claim 3,
in the step (2),
before static fermentation, adding 20L of lactobacillus acidophilus liquid, 20L of bacillus subtilis liquid, 1kg of cellulase, 2kg of ligninase and 200kg of water into every 30kg of mixture A;
after the sealing cover is removed, 1kg of cellulase and 2kg of ligninase are added into every 30kg of the mixture A.
5. The preparation method of the Chinese medicinal fermented feed additive for enhancing nutrition and treating heat stress according to claim 1,
in the step (3), the step (c),
fermenting in a sterile room for 36h at 35 ℃ without covering the fermentation container.
6. The preparation method of the Chinese medicinal fermented feed additive for enhancing nutrition and treating heat stress according to claim 5,
in the step (3), the step (c),
mixing the mixture A and the mixture B according to the mass ratio of 1:4 to obtain a mixture AA 2;
the mixture AA2 and the primary fermented mash AA are mixed according to the weight ratio of 1.5: 1-2.5: 1, mixing in a mass ratio; and 5L of lactobacillus plantarum bacterial liquid, 3L of clostridium butyricum bacterial liquid and 10L of yeast bacterial liquid are added into each 600kg of the mixture AA 2.
7. The preparation method of the Chinese medicinal fermented feed additive for enhancing nutrition and treating heat stress according to claim 1,
in the step (4), the step (c),
adding 0.2% by mass of vermiculite powder, 0.1% by mass of bentonite, 1% by mass of peptone and 1% by mass of glucose into yeast liquid, sealing a fermentation container with gauze, culturing for 16h at 28 ℃, adding 0.2% by mass of radix astragali powder, and continuously culturing for 8h to obtain a secondary yeast culture solution;
the mixture B and a secondary fermentation substrate BB are mixed according to the ratio of 1: 5-1: 7, mixing according to a mass ratio; adding 3kg of ligninase, 15L of yeast secondary culture solution and 48kg of water into every 150kg of the mixture B; fermenting the fermentation container without covering in a sterile room at 28 deg.C for 6h, and then heating to 38 deg.C for 8 h.
8. The preparation method of the Chinese medicinal fermented feed additive for enhancing nutrition and treating heat stress according to claim 1,
in the step (5), the step (c),
the static fermentation conditions were: fermenting at 30 deg.C for 2 days.
9. The preparation method of the Chinese medicinal fermented feed additive for enhancing nutrition and treating heat stress according to claim 1,
the Lactobacillus acidophilus liquid is prepared by culturing Lactobacillus acidophilus at 33 deg.C for 22 hr at 180 r/min;
the Bacillus subtilis liquid is prepared by culturing Bacillus subtilis at 33 deg.C for 18h at 180 r/min;
the lactobacillus plantarum solution is prepared by culturing lactobacillus plantarum at 33 ℃ for 18h at 180 r/min;
the clostridium butyricum bacterial liquid is prepared by culturing clostridium butyricum at 33 ℃ for 22 hours at 180 r/min;
the yeast liquid is prepared by culturing yeast at 33 deg.C for 18h at 180 r/min.
10. The preparation method of the Chinese medicinal fermented feed additive for enhancing nutrition and treating heat stress according to claim 9,
the components of the culture medium used for preparing the bacterial liquid of each bacterium are as follows: 1 percent of yeast extract, 1 percent of peptone and 4 percent of glucose.
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