CN114134027B

CN114134027B - Portable fast detector for new coronavirus

Info

Publication number: CN114134027B
Application number: CN202111453129.4A
Authority: CN
Inventors: 马晟利
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-12-01
Filing date: 2021-12-01
Publication date: 2022-09-20
Anticipated expiration: 2041-12-01
Also published as: CN114134027A

Abstract

Portable quick new corona virus detector belongs to virus check out test set technical field. The micro-flow plunger pump comprises a frame, a cover opener, a liquid-transferring mechanism, a moving platform and a micro-flow plunger pump, wherein the frame is made of sectional materials and light alloy materials and has the advantages of light weight and convenience in transportation, the outer side of the frame is wrapped by a shell, the bottom of the frame is provided with a base, the cover opener, the liquid-transferring mechanism and the moving platform are installed on the base, the cover opener and the liquid-transferring mechanism are arranged on the side face of the moving platform, a clamping rail is installed in the frame, and the micro-flow plunger pump is installed on the clamping rail in a clamping mode. The invention has smart structural design, can realize rapid screening and judgment on virus-infected dangerous people, and can realize rapid on-site detection.

Description

Portable fast detector for new coronavirus

Technical Field

The invention relates to a new coronavirus detection instrument, and belongs to the technical field of new coronavirus nucleic acid detection equipment.

Background

Nucleic acids (including DNA and RNA) are commonly used as raw materials for various analyses and experiments in medicine, pharmaceutical research, and clinical diagnostics.

In modern molecular biological assays, nucleic acid-based molecular diagnostic and detection techniques are increasingly showing crucial roles in a wide variety of fields.

For example, in the control of novel coronaviruses, early detection and early isolation of patients are the most critical. Early detection of patients relies on rapid and accurate nucleic acid detection.

In the process of preventing and controlling viruses, mainly adopted measures are isolation treatment and isolation observation of closely contacted people, nucleic acid detection is necessary in the isolation process, whether isolation personnel confirm infected viruses or not can be confirmed through nucleic acid detection, in the nucleic acid detection process, patients with symptoms suspected to be infected need to be detected in a short time in a relatively closed environment, large-scale collection and detection are necessary, but independent rapid detection is needed to be carried out on obvious symptoms, whether infection exists or not is determined, and all people in a closed space are reasonably evaluated and treated.

In addition, in the detection process of nucleic acid, a medium solution to be detected needs to be transferred, and a detection result is obtained by observing the medium solution to be detected, while most of the existing transfer processes adopt a liquid transfer device to absorb the medium solution to be detected and transfer the medium solution to a detection chip to realize detection, but the mode needs a complex mechanical structure to support the realization, and even if the method is realized, the problem that the transfer process cannot effectively overcome process pollution exists;

therefore, a high-mobility epidemic prevention detection matching device is needed, on the basis of portability, the field test of nucleic acid is realized, the transmission control of a nucleic acid detection medium is also realized, and the detection result is provided in the field of nucleic acid detection in a short time.

In addition, the following pre-developed patent technical achievements provide a technical basis for realizing the portable rapid new crown virus detector and the nucleic acid detection medium controller, and include a multi-virus nucleic acid detection visual micro-fluidic chip and a detection method thereof (202110600618.1 Libusha, Machengli and the like), a micro-channel blocking-based nucleic acid on-site rapid inspection device (2020215250462 Libusha, Zhangiangyi and the like), a nucleic acid constant-temperature amplification reaction blocking-flow-based micro-fluidic visual on-site rapid inspection device and a detection method thereof (202010747. X Libusha, Chengli and the like).

Disclosure of Invention

The present invention has been developed in order to solve the above-mentioned technical problems, and a brief summary of the present invention is given below in order to provide a basic understanding of some aspects of the present invention. It should be understood that this summary is not an exhaustive overview of the invention. It is not intended to determine the key or critical elements of the present invention, nor is it intended to limit the scope of the present invention.

The technical scheme of the invention is as follows:

a nucleic acid detection media controller, characterized by: the device comprises a three-way valve, a one-way valve, a water tank, a microfluidic chip a, a microfluidic chip b, a micro-flow plunger pump a and a micro-flow plunger pump b, wherein the three-way valve is provided with a first interface 13a, a second interface 13b and a third interface 13c, the one-way valve comprises a first one-way valve, a second one-way valve, a third one-way valve and a fourth one-way valve, the inlet of the first one-way valve is connected with a detection test tube through a rubber tube, the outlet of the first one-way valve is connected with the second interface 13b through a rubber tube, the first interface 13a is connected with the micro-flow plunger pump a, the inlet of the second one-way valve is connected with the third interface 13c through a rubber tube, the outlet of the second one-way valve is connected with the inlet of the microfluidic chip a through a rubber tube, the outlet of the microfluidic chip a is connected with the inlet of the microfluidic chip b through a rubber tube, the outlet of the microfluidic chip b is connected with the outlet of the third one-way valve, the inlet of the third one-way valve is connected with the outlet of the water tank, the inlet of the water tank is connected with the outlet of the fourth one-way valve, and the inlet of the fourth one-way valve is connected with the micro-flow plunger pump b.

Portable quick new crown virus detector, install nucleic acid detection medium controller in the detector and realize the control that the nucleic acid testing process detected liquid, which comprises a frame, the cap opener, move liquid mechanism, moving platform and micro-flow plunger pump, the frame is section bar and light alloy material, it is light to have a quality, the advantage of the transportation of being convenient for, the outside parcel of frame has the shell, the bottom of frame has the base, install the cap opener on the base, move liquid mechanism and moving platform, the cap opener arranges the side at moving platform with moving liquid mechanism, install the card rail in the frame, the micro-flow plunger pump adopts the mode that clamps to install on the card rail.

Further: in order to facilitate carrying of the new coronavirus detector, a handle is mounted at the top of the shell 6.

Further: in order to improve the detection accuracy, a fan is arranged in the inner space of the new coronavirus detection instrument, and particularly a fan is arranged on the shell.

Further: in order to avoid the propagation of the internal bacteria of the new coronavirus detector and influence the detection accuracy of other detection samples, an ultraviolet lamp with a sterilization function is arranged in the internal space of the new coronavirus detector, and the method specifically comprises the following steps: an ultraviolet lamp is installed in the frame.

Further, the method comprises the following steps: the cover opener comprises a first support, a cover opener lifting motor, a cover opener head, a guide rod and a first lead screw, wherein the first support is arranged on the base, the cover opener lifting motor is arranged on the first support, the first lead screw is arranged at the output end of the cover opener lifting motor, the cover opener head is arranged with the first lead screw in a matching way through a nut, the guide rod is also arranged on the first support, and the guide rod is arranged with the cover opener head in a matching way;

the cover opener is characterized in that a hole is formed in the cover opener head, cover opening teeth are arranged in the hole, a cover opener vertical motor is installed on the cover opener head, and the output end of the cover opener vertical motor penetrates through the cover opener head and is installed in a matched mode with the cover opening teeth.

Further: the liquid transfer mechanism comprises a second support, a liquid transfer device vertical motor, a liquid transfer device head, a liquid transfer motor, a second lead screw, a fine adjustment seat, a fine adjustment screw nut and a liquid transfer device, wherein the second support is arranged on the base, the liquid transfer device vertical motor is arranged on the second support, a first belt pulley is arranged at the output end of the liquid transfer device vertical motor, a second lead screw installation seat is arranged on the second support, a second lead screw is arranged in the second lead screw installation seat in a matched mode, a second belt pulley is arranged at the top end of the second lead screw in a matched mode, and the first belt pulley and the second belt pulley are arranged in an installation relation through a belt;

a liquid transfer motor is installed on the liquid transfer machine head, a third belt pulley is installed at the output end of the liquid transfer motor, a third lead screw is rotatably installed on the liquid transfer machine head through a bearing, a fourth belt pulley is installed on the third lead screw, and the third belt pulley and the fourth belt pulley are installed through a belt;

the fine adjustment seat is installed through the cooperation of fine setting screw and third lead screw, installs the optical axis guide arm on the pipettor aircraft nose, and the fine adjustment seat is installed with the cooperation of optical axis guide arm, the pipettor is installed on the pipettor aircraft nose, and the bottom of pipettor has the ejector pad board.

Further, the method comprises the following steps: the liquid transfer machine head is provided with a limit switch, and the fine adjustment seat is provided with a shading plate.

Further: the movable platform comprises a movable seat, a movable nut, a movable lead screw, a movable motor, a movable slide rail and a movable slide block, wherein the movable slide rail is arranged on the base in parallel, the movable seat is installed on the movable slide rail through the movable slide block, the movable nut is installed on the movable seat, the movable motor is fixedly installed on the base, the movable lead screw is installed at the output end of the movable motor, and the movable lead screw is installed in a matched mode with the movable nut.

Further: the micro-flow plunger pump comprises a micro-flow plunger pump core, a micro-flow plunger pump casing, a plunger pump driver, a fourth lead screw, an installation slider and a micro-flow plunger pump installation frame, the micro-flow plunger pump installation frame is installed on a clamping rail, the installation slider is slidably installed in the micro-flow plunger pump installation frame, the plunger pump driver is installed on the micro-flow plunger pump installation frame, the fourth lead screw is installed at the output end of the plunger pump driver, the fourth lead screw is installed through a nut and the installation slider in a matched mode, the micro-flow plunger pump casing is installed on the installation slider, one end of the micro-flow plunger pump core is installed in the micro-flow plunger pump casing, and the other end of the micro-flow plunger pump core is fixed on the micro-flow plunger pump installation frame.

Further: in order to ensure the personal safety of personnel participating in the detection, a defense device is further installed on the outer side of the new coronavirus detection instrument, the defense device is a glove box, the glove box is installed on the side wall of the frame, an operation hole is formed in the glove box, and a protective glove is installed on the operation hole.

The invention has the following beneficial effects:

1. the nucleic acid detection medium controller provided by the invention realizes the circulation control of a nucleic acid detection medium, can effectively ensure that a medium solution to be detected does not leak or enter, and ensures the accuracy and reliability of a nucleic acid detection process.

1. The invention provides a portable rapid new coronavirus detector, which is characterized in that a battery is arranged in the portable rapid new coronavirus detector, internal power supply is realized by using the battery, and the portable rapid new coronavirus detector is free of a power line, convenient to carry, long in endurance time and capable of being used repeatedly.

2. The invention provides a portable rapid detector for new coronavirus, which has a firm box body structure, uses a large amount of sectional materials and light alloy, has light weight and is convenient to transport.

3. The invention provides a portable rapid detector for new coronavirus, which is internally provided with a special medical sterilizing device meeting the biological safety SP2 standard.

4. The invention provides a portable rapid new coronavirus detector which is automatically controlled by a PLC (programmable logic controller), completes all actions by one-time operation, reduces the contact frequency of personnel and a sample, and reduces the propagation risk.

5. The portable rapid detector for the new coronavirus has the functions of uncovering, pipetting and detecting, can rapidly realize field detection, and when the device is applied to nucleic acid detection of centralized isolation personnel, detection workers can carry the device at will, and utilize the device to complete rapid nucleic acid detection of the isolation personnel in each isolation room one by one, and then a detection result is issued.

6. The portable rapid detector for the new corona virus can realize rapid uncovering of the acquisition test tube through the uncovering mechanism, and the uncovering action is realized in a mechanical mode, so that the risk of personnel infection caused by leakage of a detection sample due to manual operation is avoided.

7. The liquid transfer mechanism of the portable rapid new coronavirus detector has the advantage of flexible structure, can rapidly complete the transfer and collection of a detected sample through the liquid transfer mechanism, and can automatically push a used needle into a sample test tube after the liquid transfer mechanism finishes the work, so that the problem of sample detection error caused by cross infection of the sample is avoided;

8. the invention has ingenious structural design, can realize rapid screening and judgment on virus-infected dangerous people, and can realize rapid detection within 30 minutes on visual site;

9. the portable rapid new corona virus detector can rapidly complete new corona screening and judgment, shorten the detection time, reduce the cost and realize port forward movement and community screening of the new corona screened by hospitals. The invention is expected to solve the problem that the primary medical institutions such as community and town health institutions do not have detection conditions; the first line of epidemic prevention such as railway stations, airports, places for near inspection and the like is difficult to carry out detection; the traditional inactivation method needs 1 hour, and nucleic acid pollution and other problems can be caused after amplification. Can provide technical support for global epidemic outbreak, provides a new idea for subsequent novel coronavirus screening, prevention and treatment, and has positive guarantee function and great prevention and control significance.

Drawings

FIG. 1 is a piping connection diagram of a nucleic acid detecting medium controller;

FIG. 2 is a perspective view of a portable rapid new coronavirus detection instrument;

FIG. 3 is a front view of the portable rapid new corona virus detection instrument;

FIG. 4 is a perspective view showing the mounting positions of the lid opener, the pipetting mechanism and the moving platform;

FIG. 5 is a perspective view of the glove box;

FIG. 6 is a perspective view of the pipetting mechanism;

fig. 7 is a perspective view of the lid opener;

FIG. 8 is a perspective view of the lid opener head;

FIG. 9 is a perspective view of a micro-flow plunger pump;

FIG. 10 is a schematic view of the mounting of a three-way valve and a one-way valve on a mobile platform;

in the figure, 1, a frame, 2, a cap opener, 3, a liquid transfer mechanism, 4, a moving platform, 5, a micro-flow plunger pump, 6, a shell, 7, a base, 8, a clamping rail, 9, a lifting handle, 10, a fan, 11, an ultraviolet lamp, 12-a glove box, 13, a three-way valve, 14, a one-way valve, 15, a heater, 16, a water tank, 17, a micro-fluidic chip a, 18, a micro-fluidic chip b, 2-1, a first support, 2-2, a cap opener lifting motor, 2-3, a cap opener head, 2-4, a guide rod, 2-5, a first lead screw, 2-6, a cap opener vertical motor, 2-7, a cap opener tooth, 3-1, a second support, 3-2, a liquid transfer vertical motor, 3-3, a liquid transfer machine head, 3-4, a liquid transfer motor, 3-5 and a second lead screw, 3-6 parts of fine adjustment seat, 3-7 parts of fine adjustment screw, 3-8 parts of pipettor, 3-9 parts of second screw rod installation seat, 3-10 parts of first belt pulley, 3-11 parts of second belt pulley, 3-12 parts of third belt pulley, 3-13 parts of fourth belt pulley, 3-14 parts of third screw rod, 3-15 parts of optical axis guide rod, 3-16 parts of ejector pin plate, 3-17 parts of limit switch, 3-18 parts of shading plate, 4-1 parts of moving seat, 4-2 parts of moving screw rod, 4-3 parts of moving screw rod, 4-4 parts of moving motor, 4-5 parts of moving slide rail, 4-6 parts of moving slide block, 5-1 parts of micro-flow plunger pump core, 5-2 parts of micro-flow plunger pump shell, 5-3 parts of plunger pump driver, 5-4 parts of plunger pump, 5-4 parts of micro-flow plunger pump, 5-5 parts of a fourth lead screw, 5-6 parts of a mounting slide block and a mounting rack of the micro-flow plunger pump.

Detailed Description

In order that the objects, aspects and advantages of the invention will become more apparent, the invention will be described by way of example only, and in connection with the accompanying drawings. It is to be understood that such description is merely illustrative and not intended to limit the scope of the present invention. Moreover, in the following description, descriptions of well-known structures and techniques are omitted so as to not unnecessarily obscure the concepts of the present invention.

The connection mentioned in the invention is divided into fixed connection and detachable connection, the fixed connection is non-detachable connection and includes but is not limited to folding edge connection, rivet connection, bonding connection, welding connection and other conventional fixed connection modes, the detachable connection includes but is not limited to threaded connection, snap connection, pin connection, hinge connection and other conventional detachment modes, when the specific connection mode is not clearly limited, at least one connection mode can be found in the existing connection modes by default to realize the function, and the skilled person can select according to the needs. For example: the fixed connection selects welding connection, and the detachable connection selects hinge connection.

The first embodiment is as follows: referring to fig. 1, the nucleic acid detecting medium controller includes a three-way valve 13, a one-way valve 14, a water tank 16, a microfluidic chip a17, a microfluidic chip b18, a microfluidic plunger pump a, and a microfluidic plunger pump b, the three-way valve 13 has a first port 13a, a second port 13b, and a third port 13c, the one-way valve 14 includes a first one-way valve 14-1, a second one-way valve 14-2, a third one-way valve 14-3, and a fourth one-way valve 14-4, an inlet of the first one-way valve 14-1 is connected to a detection test tube through a rubber tube, an outlet of the first one-way valve 14-1 is connected to the second port 13b through a rubber tube, the first port 13a is connected to the microfluidic plunger pump a, an inlet of the second one-way valve 14-2 is connected to the third port 13c through a rubber tube, an outlet of the second one-way valve 14-2 is connected to an inlet of the microfluidic chip a17 through a rubber tube, an outlet of the microfluidic chip a17 is connected with an inlet of the microfluidic chip b18 through a rubber tube, an outlet of the microfluidic chip b18 is connected with an outlet of a third one-way valve 14-3, an inlet of the third one-way valve 14-3 is connected with an outlet of a water tank 16, an inlet of the water tank 16 is connected with an outlet of a fourth one-way valve 14-4, and an inlet of the fourth one-way valve 14-4 is connected with a micro-flow plunger pump b.

The control principle of the nucleic acid detection medium controller is as follows: firstly, detecting a medium solution to be detected in a test tube, reversely rotating a driver of a micro-flow plunger pump a to enable the medium solution to be detected to enter a three-way valve from a second interface 13b of the three-way valve 13, sending the medium solution to be detected to a micro-flow chip a17 through a third interface 13c and a second one-way valve 14-2 of the three-way valve 13, simultaneously sucking liquid in a water tank 16 through a fourth one-way valve 14-4 by the micro-flow plunger pump b, keeping the internal and external pressure of the micro-flow chip b (enabling the micro-flow chip b to be in a negative pressure state and enabling the micro-flow chip b to suck the detection liquid from the micro-flow chip a) because an outlet end of the water tank 16 is communicated with an outlet of the micro-flow chip b through the third one-way valve 14-3), observing a picture of the micro-flow microscope 19 output chip b, detecting a sample, pressurizing the micro-flow plunger pump b of the water tank 16 to the water tank after detection is finished, the detection solution is returned to the microfluidic chip a.

In the embodiment, the circulation control of the medium solution to be detected is realized through the nucleic acid detection medium controller, in the detection process, the medium solution to be detected realizes sample detection in the microfluidic chip b, and a detection result is provided, the microfluidic chip a is used as an intermediate transmission temporary tool and is also used as a sample waste collection tool after detection, so that the problem that virus cross infection is caused by a single microfluidic chip when next sample detection is carried out after one sample detection is finished and the detection accuracy is influenced is solved; and after the detection is finished, the microfluidic chip a is taken out of the detection equipment and is reasonably disposed.

In addition, because the micro-flow plunger pump b, the fourth one-way valve 14-4, the water tank 16 and the third one-way valve 14-3 are arranged in the nucleic acid detection medium controller, under the action of the micro-flow plunger pump b, the micro-flow chip b18 can be flushed by water in the water tank 16, so that the cleanness of the micro-flow chip b18 before each use is kept, and the detection accuracy is improved. The specific implementation mode is that the micro-flow plunger pump b pressurizes the water tank 16, and water in the water tank 16 enters the micro-fluidic chip b from the third one-way valve 14-3 to realize the cleaning of the micro-fluidic chip b.

The embodiment realizes the circulation control of the nucleic acid detection medium through the connection relation structure of the one-way valve, the three-way valve, the flow plunger pump and the water tank, can effectively ensure that the solution of the medium to be detected does not leak or flow into the detection system, and ensures the accuracy and reliability of the nucleic acid detection process.

The second embodiment is as follows: the portable fast detector for detecting the new corona virus comprises a frame 1, a cap opener 2, a liquid transfer mechanism 3, a moving platform 4 and a micro-flow plunger pump 5, wherein the frame is made of sectional materials and light alloy materials and has the advantages of light weight and convenience in transportation, a shell 6 wraps the outer side of the frame 1, a base 7 is arranged at the bottom of the frame 1, the cap opener 2, the liquid transfer mechanism 3 and the moving platform 4 are installed on the base 7, the cap opener 2 and the liquid transfer mechanism 3 are arranged on the side face of the moving platform 4, a clamping rail 8 is installed in the frame 1, and the micro-flow plunger pump 5 is installed on the clamping rail 8 in a clamping mode.

The cap opener 2 comprises a first support 2-1, a cap opener lifting motor 2-2, a cap opener head 2-3, a guide rod 2-4 and a first lead screw 2-5, the first support 2-1 is installed on a base 7, the cap opener lifting motor 2-2 is installed on the first support 2-1, the first lead screw 2-5 is installed at the output end of the cap opener lifting motor 2-2, the cap opener head 2-3 is installed with the first lead screw 2-5 in a matched mode through a nut, the guide rod 2-4 is further installed on the first support 2-1, and the guide rod 2-4 is installed with the cap opener head 2-3 in a matched mode;

the cover opening machine head 2-3 is provided with a hole, the hole is provided with cover opening teeth 2-7, the cover opening device vertical motor 2-6 is arranged on the cover opening machine head 2-3, and the output end of the cover opening device vertical motor 2-6 penetrates through the cover opening machine head 2-3 to be matched with the cover opening teeth 2-7 for installation

The liquid transfer mechanism 3 comprises a second support 3-1, a liquid transfer vertical motor 3-2, a liquid transfer machine head 3-3, a liquid transfer motor 3-4, a second screw rod 3-5, a fine adjustment seat 3-6, a fine adjustment nut 3-7 and a liquid transfer machine 3-8, the second support 3-1 is arranged on a base 7, the liquid transfer vertical motor 3-2 is arranged on the second support 3-1, a first belt pulley 3-10 is arranged at the output end of the liquid transfer vertical motor 3-2, a second lead screw mounting seat 3-9 is mounted on the second support 3-1, a second lead screw 3-5 is mounted in the second lead screw mounting seat 3-9 in a matching manner, a second belt pulley 3-11 is mounted at the top end of the second lead screw 3-5 in a matching manner, and the first belt pulley 3-10 is mounted with the second belt pulley 3-11 through a belt; a longitudinal moving guide rail 3-20 is arranged in the vertical direction of the second support 3-1, and the liquid transfer machine head 3-3 slides on the longitudinal moving guide rail 3-20 through a sliding block;

a liquid-moving motor 3-4 is installed on the liquid-moving machine head 3-3, a third belt pulley 3-12 is installed at the output end of the liquid-moving motor 3-4, a third lead screw 3-14 is installed on the liquid-moving machine head 3-3 in a rotating mode through a bearing, a fourth belt pulley 3-13 is installed on the third lead screw 3-14, and the third belt pulley 3-12 is in installation relation with the fourth belt pulley 3-13 through a belt;

the fine adjustment seat 3-6 is matched and installed with a third screw rod 3-14 through a fine adjustment nut 3-7, an optical axis guide rod 3-15 is installed on the liquid transfer machine head 3-3, the fine adjustment seat 3-6 is matched and installed with the optical axis guide rod 3-15, the liquid transfer machine 3-8 is installed on the liquid transfer machine head 3-3, and a push pin plate 3-16 is arranged at the bottom of the liquid transfer machine 3-8; the ejector plate 3-16 is used for enabling the needle heads 3-19 to fall into the sample test tube, so that the problem of sample detection error caused by cross infection of samples is avoided;

a limit switch 3-17 is arranged on the pipette head 3-3, and a light screen 3-18 is arranged on the fine adjustment seat 3-6;

the movable platform 4 comprises a movable seat 4-1, a movable screw nut 4-2, a movable lead screw 4-3, a movable motor 4-4, a movable slide rail 4-5 and a movable slide block 4-6, two movable slide rails 4-5 arranged in parallel are installed on a base 7, the movable seat 4-1 is installed on the movable slide rail 4-5 through the movable slide block 4-6, the movable screw nut 4-2 is installed on the movable seat 4-1, the movable motor 4-4 is fixedly installed on the base 7, the movable lead screw 4-3 is installed at the output end of the movable motor 4-4, and the movable lead screw 4-3 is installed in a matched mode with the movable screw nut 4-2.

The micro-flow plunger pump 5 comprises a micro-flow plunger pump core 5-1, a micro-flow plunger pump casing 5-2, a plunger pump driver 5-3, a fourth lead screw 5-4, an installation slide block 5-5 and a micro-flow plunger pump installation frame 5-6, wherein the micro-flow plunger pump installation frame 5-6 is installed on a clamping rail 8, the micro-flow plunger pump installation frame 5-6 is internally provided with the slide block 5-5 in a sliding way, the micro-flow plunger pump installation frame 5-6 is provided with the plunger pump driver 5-3, the output end of the plunger pump driver 5-3 is provided with the fourth lead screw 5-4, the fourth lead screw 5-4 is installed with the installation slide block 5-5 in a matching way through a screw nut, the micro-flow plunger pump casing 5-2 is installed on the installation slide block 5-5, one end of the micro-flow plunger pump core 5-1 is installed in the micro-flow plunger pump casing 5-2, the other end of the micro-flow plunger pump core 5-1 is fixed on the micro-flow plunger pump mounting rack 5-6.

The inside of frame 1 still has PLC controller and battery, and the PLC controller is installed on card rail 8, and the battery is used for the power supply of each part in frame 1.

The portable rapid detector for the new coronavirus also comprises a three-way valve 13, a one-way valve 14, a heater 15, a water tank 16, a micro-fluidic chip a17 and a micro-fluidic chip b18, wherein the water tank 16 is arranged at the bottom of the frame 1, the heater 15 is arranged on the water tank 16, the micro-fluidic chip a is arranged on the movable sliding block 4-6, the micro-fluidic chip b18 is arranged on the base 7, a microscope 19 is also arranged on the base 7, and the microscope 19 is used for detecting a nucleic acid detection result;

the three-way valve 13 comprises a first interface 13a, a second interface 13b and a third interface 13c, the one-way valve 14 comprises a first one-way valve 14-1, a second one-way valve 14-2, a third one-way valve 14-3 and a fourth one-way valve 14-4, the inlet of the first one-way valve 14-1 is connected with the detection test tube through a rubber tube, the outlet of the first one-way valve 14-1 is connected with the second interface 13b through a rubber tube, the first interface 13a is connected with the micro-flow plunger pump a, the inlet of the second one-way valve 14-2 is connected with the third interface 13c through a rubber tube, the outlet of the second one-way valve 14-2 is connected with the inlet of the micro-flow control chip a17 through a rubber tube, the outlet of the micro-flow control chip a17 is connected with the inlet of the micro-flow control chip b18 through a rubber tube, the outlet of the micro-flow control chip b18 is connected with the outlet of the third one-way valve 14-3, the inlet of the third one-way valve 14-3 is connected with the outlet of the water tank 16, the inlet of the water tank 16 is connected to the outlet of the fourth check valve 14-4, and the inlet of the fourth check valve 14-4 is connected to the micro flow plunger pump b.

The working principle of the portable rapid new coronavirus detector is as follows:

the power supply of an operation button is switched on, a PLC is powered on, each device is initialized, a fan 10 is started to form negative pressure in a detection box, an ultraviolet lamp 11 is started to sterilize and disinfect the internal environment, a program is started, the ultraviolet lamp 11, a mobile platform 4 is taken out of the bin, the position of the mobile platform is fed back in a limiting way, whether the mobile platform is taken out of the bin or not is carried out, a bin door (a bin door is arranged on a shell 6 and is not shown in the figure) is opened, a sample test tube is put in, the bin door is closed, the detection environment meets the detection standard, the detection test tube is put in a test tube rack by using gloves on a glove box, a key is pressed again, a tray returns to the bin, whether the position is fed back to the position of opening the bin or not is reached, an opening device 2 carries out opening action (an opening device lifting motor 2-2 directly drives a first lead screw 2-5 to drive an opening device head 2-3 to descend to the position of a test tube cover, the tube cover is opened by a vertical motor 2-6 to rotate the opening device to open the test tube cover teeth 2-7 to open the test tube cover, a lifting motor 2-2 of the cap opener is started to reversely rotate in a delayed mode to drive a cap opening machine head to ascend to an original position), the feedback action of the cap opener 2 is completed, a moving motor 4-4 drives a moving screw rod 4-3 to rotate, a moving seat 4-1 is driven to move to the position of a liquid transfer machine 3 under the action of the moving screw rod 4-3, the limiting feedback is in place, the liquid transfer action is started, (a vertical liquid transfer machine 3-2 drives a second screw rod 3-5 through a synchronous belt to drive the liquid transfer machine head 3-3 to descend to the position of a needle head for taking the needle head 3-19, the vertical liquid transfer machine 3-2 of the liquid transfer machine head reversely rotates, the liquid transfer machine head 3-3 ascends, the moving screw rod 4-3 drives the moving seat 4-1 to enable a sample test tube arranged on the moving seat 4-1 to be positioned under the needle head 3-19 for limiting feedback, a liquid moving machine head 3-3 descends to enable a needle head 3-19 to be inserted below the liquid level of a sample, a liquid moving motor 3-4 drives a third screw rod 3-14 to rotate through a synchronous belt, the liquid moving machine head 3-8 sucks the sample, then the liquid moving machine head 3-3 moves upwards to enable the needle head to be completely separated from the test tube, the screw rod 4-3 is moved to drive a moving seat 4-1 to enable a detection test tube to move to the position right below the needle head, the liquid moving machine head 3-3 descends, the liquid moving motor 3-4 reversely rotates to push the sample out, the sample enters the detection test tube, the liquid moving machine head 3-3 moves upwards to enable the needle head to be completely separated from the detection test tube, the screw rod 4-3 is moved to drive the moving seat 4-1 to enable the sample test tube to be right opposite to the needle head, the liquid moving motor 3-4 reversely rotates to drive a push needle plate 3-16 to push the needle head to enable the needle head to fall into the sample test tube, the limit feedback is whether the liquid transfer is finished or not, after the liquid transfer is finished, the PLC controls the plunger pump driver to drive the micro-flow plunger pump 5, (the micro-flow plunger pump core 5-1 is fixed on a micro-flow plunger pump mounting frame 5-6, the micro-flow plunger pump shell 5-2 is fixed on a mounting slide block 5-5, the plunger pump driver 5-3 drives the fourth lead screw 5-4 to drive the mounting slide block 5-5 to move) to absorb the solution in the detection test tube, after the detection solution passes through the valve body through the three-way valve 13, the driver of the micro-flow plunger pump a reverses to send the detection solution out of the other outlet of the three-way valve 13 to the micro-flow chip a, and the micro-flow plunger pump b absorbs the liquid in the water tank to keep the internal and external pressure of the micro-flow chip b, (so that the micro-flow plunger chip b is in a negative pressure state, and the micro-flow plunger pump b absorbs the detection liquid from the micro-flow plunger chip a), observing the picture of the micro-fluidic chip b output by the microscope 19, detecting the check sample, after the detection is finished, pressurizing the water tank by the micro-flow plunger pump b of the water sucking tank 16, sending the detection solution back to the micro-fluidic chip a, simultaneously moving the slide block 4-6 to the position of the cover opener 2, executing the cover closing action (the cover opener lifting motor 2-2 drives the cover opener head to descend to the test tube port, the cover opener vertical motor 2-6 reversely rotates to screw the test tube cover, the limit feedback cover closing is finished, the cover opener lifting motor 2-2 drives the cover opener head to ascend to the limit, the cover closing is finished), moving the slide block 4-6 out of the bin, taking out the test tube with the needle head and the micro-fluidic chip a from the glove box 10, pressing the action key again, starting the ultraviolet lamp, and enabling the system resetting position to be zero.

The third concrete implementation mode: in the portable rapid new coronavirus detector of the present embodiment, in order to facilitate carrying of the new coronavirus detector, a handle 9 is installed at the top of the housing 6, and a worker can carry the new coronavirus detector by the handle 9 to perform a screening operation.

The fourth concrete implementation mode: in order to improve the detection accuracy, the portable fast new coronavirus detecting instrument of the embodiment is provided with a fan in the inner space, specifically, the fan 10 is arranged on the housing 6, specifically, the fan 10 is a negative pressure fan, the negative pressure fan provides negative pressure for the whole detection environment, and the dust, impurities, bacteria and the like in the instrument are removed to the outside of the detection environment, so that the detection accuracy is improved.

The fifth concrete implementation mode: the portable fast new coronavirus detecting instrument of the present embodiment is described with reference to fig. 1 to 10, which avoids the propagation of bacteria inside the new coronavirus detecting instrument and affects the detection accuracy of other detected samples, and an ultraviolet lamp with a sterilization function is disposed in the internal space of the new coronavirus detecting instrument, specifically: an ultraviolet lamp 11 is installed in the frame 1.

The sixth specific implementation mode: in order to ensure the personal safety of the personnel involved in the examination, the portable rapid new coronavirus detection instrument of the embodiment is further provided with a defense device at the outer side of the new coronavirus detection instrument, specifically, the defense device is a glove box 12, the glove box 12 is installed on the side wall of the frame 1, the glove box 12 is provided with an operation hole, a protective glove is installed on the operation hole, the glove box 12 is further provided with a switch door, and the test tube and the microfluidic chip can be taken out or placed through the switch door.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.

The relative arrangement of the components and steps, the numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention unless specifically stated otherwise. Meanwhile, it should be understood that the sizes of the respective portions shown in the drawings are not drawn in an actual proportional relationship for the convenience of description. Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail but are intended to be part of the specification where appropriate. In all examples shown and discussed herein, any particular value should be construed as merely illustrative, and not limiting. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, further discussion thereof is not required in subsequent figures.

In the description of the present invention, it is to be understood that the orientation or positional relationship indicated by the orientation words such as "front, rear, upper, lower, left, right", "lateral, vertical, horizontal" and "top, bottom", etc. are usually based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and in the case of not making a reverse description, these orientation words do not indicate and imply that the device or element being referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore, should not be considered as limiting the scope of the present invention; the terms "inner and outer" refer to the inner and outer relative to the profile of the respective component itself.

Spatially relative terms, such as "above … …," "above … …," "above … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial relationship to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if a device in the figures is turned over, devices described as "above" or "on" other devices or configurations would then be oriented "below" or "under" the other devices or configurations. Thus, the exemplary term "above … …" can include both an orientation of "above … …" and "below … …". The device may be otherwise variously oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.

It should be noted that the terms "first," "second," and the like in the description and claims of this application and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the application described herein are capable of operation in sequences other than those illustrated or described herein.

It should be noted that, in the above embodiments, as long as the technical solutions can be aligned and combined without contradiction, those skilled in the art can exhaust all possibilities according to the mathematical knowledge of the alignment and combination, and therefore, the present invention does not describe the technical solutions after alignment and combination one by one, but it should be understood that the technical solutions after alignment and combination have been disclosed by the present invention.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. Portable quick new corona virus detector, its characterized in that: the micro-flow plunger pump comprises a frame (1), a cover opener (2), a liquid-transferring mechanism (3), a mobile platform (4) and a micro-flow plunger pump (5), wherein a shell (6) is wrapped on the outer side of the frame (1), a base (7) is arranged at the bottom of the frame (1), the cover opener (2), the liquid-transferring mechanism (3) and the mobile platform (4) are installed on the base (7), the cover opener (2) and the liquid-transferring mechanism (3) are arranged on the side surface of the mobile platform (4), a clamping rail (8) is installed in the frame (1), and the micro-flow plunger pump (5) is installed on the clamping rail (8);

the moving platform (4) comprises a moving seat (4-1), a moving nut (4-2), a moving screw rod (4-3), a moving motor (4-4), a moving slide rail (4-5) and a moving slide block (4-6), two parallel movable sliding rails (4-5) are arranged on a base (7), a movable base (4-1) is arranged on the movable sliding rails (4-5) through movable sliding blocks (4-6), a movable screw nut (4-2) is arranged on the movable base (4-1), a movable motor (4-4) is fixedly arranged on the base (7), a movable lead screw (4-3) is arranged at the output end of the movable motor (4-4), and the movable lead screw (4-3) and the movable screw nut (4-2) are installed in a matched mode;

the micro-flow plunger pump (5) comprises a micro-flow plunger pump core (5-1), a micro-flow plunger pump casing (5-2), a plunger pump driver (5-3), a fourth lead screw (5-4), a mounting slide block (5-5) and a micro-flow plunger pump mounting rack (5-6), wherein the micro-flow plunger pump mounting rack (5-6) is mounted on a clamping rail (8), the mounting slide block (5-5) is slidably mounted in the micro-flow plunger pump mounting rack (5-6), the plunger pump driver (5-3) is mounted on the micro-flow plunger pump mounting rack (5-6), the fourth lead screw (5-4) is mounted at the output end of the plunger pump driver (5-3), and the fourth lead screw (5-4) is mounted with the mounting slide block (5-5) in a matching manner through a nut, a micro-flow plunger pump casing (5-2) is arranged on the mounting slide block (5-5), one end of a micro-flow plunger pump core (5-1) is arranged in the micro-flow plunger pump casing (5-2), the other end of the micro-flow plunger pump core (5-1) is fixed on a micro-flow plunger pump mounting frame (5-6), and the micro-flow plunger pump further comprises a glove box 12, and the glove box 12 is arranged on the side wall of the frame (1);

further comprising: the micro-flow plunger pump comprises a three-way valve (13), a one-way valve (14), a water tank (16), a micro-flow chip a (17), a micro-flow chip b (18), a micro-flow plunger pump a and a micro-flow plunger pump b, wherein the three-way valve (13) is provided with a first interface (13 a), a second interface (13 b) and a third interface (13 c), the one-way valve (14) comprises a first one-way valve (14-1), a second one-way valve (14-2), a third one-way valve (14-3) and a fourth one-way valve (14-4), the inlet of the first one-way valve (14-1) is connected with a detection test tube through a rubber tube, the outlet of the first one-way valve (14-1) is connected with the second interface (13 b) through a rubber tube, the first interface (13 a) is connected with the micro-flow plunger pump a, the inlet of the second one-way valve (14-2) is connected with the third interface (13 c) through a rubber tube, an outlet of the second one-way valve (14-2) is connected with an inlet of a micro-fluidic chip a (17) through a rubber tube, an outlet of the micro-fluidic chip a (17) is connected with an inlet of a micro-fluidic chip b (18) through a rubber tube, an outlet of the micro-fluidic chip b (18) is connected with an outlet of a third one-way valve (14-3), an inlet of the third one-way valve (14-3) is connected with an outlet of a water tank (16), an inlet of the water tank (16) is connected with an outlet of a fourth one-way valve (14-4), and an inlet of the fourth one-way valve (14-4) is connected with a micro-flow plunger pump b;

the water tank (16) is arranged at the bottom of the frame (1), the micro-fluidic chip a (17) is arranged on the movable sliding block (4-6), the micro-fluidic chip b (18) is arranged on the base (7), the micro-flow plunger pump (5) comprises a micro-flow plunger pump a and a micro-flow plunger pump b, the base (7) is further provided with a microscope (19), and the microscope (19) is used for detecting a nucleic acid detection result.

2. The portable rapid new coronavirus detection instrument of claim 1, wherein: and a handle (9) is arranged at the top of the shell (6).

3. The portable rapid new coronavirus detection instrument of claim 1, wherein: and a fan (10) is arranged on the shell (6).

4. The portable rapid new coronavirus detection instrument of claim 1, wherein: an ultraviolet lamp (11) is installed in the frame (1).

5. The portable rapid new coronavirus detection instrument of any one of claims 1-4, wherein: the cap opener (2) comprises a first support (2-1), a cap opener lifting motor (2-2), a cap opener head (2-3), a guide rod (2-4) and a first lead screw (2-5), the first support (2-1) is installed on a base (7), the cap opener lifting motor (2-2) is installed on the first support (2-1), the first lead screw (2-5) is installed at the output end of the cap opener lifting motor (2-2), the cap opener head (2-3) is installed with the first lead screw (2-5) in a matched mode through a screw, the guide rod (2-4) is further installed on the first support (2-1), and the guide rod (2-4) is installed with the cap opener head (2-3) in a matched mode;

the cover opening machine head (2-3) is provided with a hole, cover opening teeth (2-7) are arranged in the hole, a cover opening device vertical motor (2-6) is installed on the cover opening machine head (2-3), and the output end of the cover opening device vertical motor (2-6) penetrates through the cover opening machine head (2-3) and is installed in a matched mode with the cover opening teeth (2-7).

6. The portable rapid new coronavirus detection instrument of any one of claims 1-4, wherein: the liquid transfer mechanism (3) comprises a second support (3-1), a liquid transfer vertical motor (3-2), a liquid transfer machine head (3-3), a liquid transfer motor (3-4), a second lead screw (3-5), a fine adjustment seat (3-6), a fine adjustment nut (3-7) and a liquid transfer machine (3-8), the second support (3-1) is installed on the base (7), the liquid transfer vertical motor (3-2) is installed on the second support (3-1), a first belt pulley (3-10) is installed at the output end of the liquid transfer vertical motor (3-2), a second lead screw installation seat (3-9) is installed on the second support (3-1), and the second lead screw (3-5) is installed in the second lead screw installation seat (3-9) in a matching manner, a second belt pulley (3-11) is arranged at the top end of the second lead screw (3-5) in a matching way, and the first belt pulley (3-10) is in installation relation with the second belt pulley (3-11) through a belt;

a liquid transfer motor (3-4) is installed on the liquid transfer machine head (3-3), a third belt pulley (3-12) is installed at the output end of the liquid transfer motor (3-4), a third lead screw (3-14) is installed on the liquid transfer machine head (3-3) through a bearing in a rotating mode, a fourth belt pulley (3-13) is installed on the third lead screw (3-14), and the third belt pulley (3-12) is in installation relation with the fourth belt pulley (3-13) through a belt;

the fine adjustment seat (3-6) is installed with a third lead screw (3-14) in a matched mode through a fine adjustment screw nut (3-7), an optical axis guide rod (3-15) is installed on the liquid transfer machine head (3-3), the fine adjustment seat (3-6) is installed with the optical axis guide rod (3-15) in a matched mode, the liquid transfer machine (3-8) is installed on the liquid transfer machine head (3-3), and a needle pushing plate (3-16) is arranged at the bottom of the liquid transfer machine (3-8).

7. The portable rapid new coronavirus detection instrument of claim 6, wherein: the liquid transfer machine head (3-3) is provided with a limit switch (3-17), and the fine adjustment seat (3-6) is provided with a light screen (3-18).