CN114107460A - 一种基因抑制剂在抑制脐带血间充质干细胞衰老中的应用 - Google Patents
一种基因抑制剂在抑制脐带血间充质干细胞衰老中的应用 Download PDFInfo
- Publication number
- CN114107460A CN114107460A CN202111415058.9A CN202111415058A CN114107460A CN 114107460 A CN114107460 A CN 114107460A CN 202111415058 A CN202111415058 A CN 202111415058A CN 114107460 A CN114107460 A CN 114107460A
- Authority
- CN
- China
- Prior art keywords
- lnc
- stem cells
- mesenchymal stem
- cord blood
- umbilical cord
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 62
- 210000004700 fetal blood Anatomy 0.000 title claims abstract description 58
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 44
- 239000003112 inhibitor Substances 0.000 title claims abstract description 34
- 230000032683 aging Effects 0.000 title claims abstract description 15
- 230000005764 inhibitory process Effects 0.000 title description 4
- 230000009758 senescence Effects 0.000 claims abstract description 16
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 14
- 210000004027 cell Anatomy 0.000 claims description 27
- 108091027967 Small hairpin RNA Proteins 0.000 claims description 16
- 239000003814 drug Substances 0.000 claims description 6
- 101150084750 1 gene Proteins 0.000 claims description 4
- 239000008194 pharmaceutical composition Substances 0.000 claims description 3
- 239000003937 drug carrier Substances 0.000 claims 1
- 230000008943 replicative senescence Effects 0.000 abstract description 16
- 238000002474 experimental method Methods 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 6
- 108091027963 non-coding RNA Proteins 0.000 abstract description 6
- 102000042567 non-coding RNA Human genes 0.000 abstract description 6
- 230000036542 oxidative stress Effects 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 4
- 102000005936 beta-Galactosidase Human genes 0.000 description 18
- 108010005774 beta-Galactosidase Proteins 0.000 description 18
- 108020004414 DNA Proteins 0.000 description 14
- 230000035755 proliferation Effects 0.000 description 12
- 239000012192 staining solution Substances 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 238000004113 cell culture Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108010058546 Cyclin D1 Proteins 0.000 description 5
- 102100037858 G1/S-specific cyclin-E1 Human genes 0.000 description 5
- 101000738568 Homo sapiens G1/S-specific cyclin-E1 Proteins 0.000 description 5
- 238000001890 transfection Methods 0.000 description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 102100024165 G1/S-specific cyclin-D1 Human genes 0.000 description 4
- 230000033228 biological regulation Effects 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 238000003753 real-time PCR Methods 0.000 description 4
- 210000000130 stem cell Anatomy 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 239000002502 liposome Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000010839 reverse transcription Methods 0.000 description 3
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 108091092584 GDNA Proteins 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 239000002033 PVDF binder Substances 0.000 description 2
- 102000006382 Ribonucleases Human genes 0.000 description 2
- 108010083644 Ribonucleases Proteins 0.000 description 2
- 108091046869 Telomeric non-coding RNA Proteins 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000008346 aqueous phase Substances 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 239000000834 fixative Substances 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 108010087230 Sincalide Proteins 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 238000010609 cell counting kit-8 assay Methods 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
- 238000012165 high-throughput sequencing Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000005087 mononuclear cell Anatomy 0.000 description 1
- 210000001178 neural stem cell Anatomy 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 238000012257 pre-denaturation Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- IZTQOLKUZKXIRV-YRVFCXMDSA-N sincalide Chemical compound C([C@@H](C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(N)=O)NC(=O)[C@@H](N)CC(O)=O)C1=CC=C(OS(O)(=O)=O)C=C1 IZTQOLKUZKXIRV-YRVFCXMDSA-N 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
- C12N2310/113—Antisense targeting other non-coding nucleic acids, e.g. antagomirs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Developmental Biology & Embryology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Rheumatology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Plant Pathology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Description
试剂 | 添加量 |
5×gDNA Eraser Buffer | 2μl |
gDNA Eraser | 1μl |
Total RNA | 1μg |
RNase Free dH2O | up to 10μl |
试剂 | 添加量 |
步骤(7)反应液 | 10μl |
PrimeScript RT Enzyme Mix I | 1μl |
RT Primer Mix | 1μl |
5×PrimeScript Buffer | 4μl |
RNase Free dH2O | 4μl |
试剂 | 添加量 |
SYBR Green Premix Ex Taq(2×) | 10μl |
上游引物 | 0.4μl |
下游引物 | 0.4μl |
cDNA template | 2μl |
ddH2O | 4μl |
Sh-NC | Sh-lnc-AC005307.1 | |
1d | 0.240±0.006 | 0.234±0.012 |
2d | 0.291±0.024 | 0.368±0.029 |
3d | 0.363±0.027 | 0.586±0.036 |
4d | 0.429±0.025 | 0.706±0.040 |
5d | 0.467±0.035 | 0.790±0.039 |
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111415058.9A CN114107460A (zh) | 2021-11-25 | 2021-11-25 | 一种基因抑制剂在抑制脐带血间充质干细胞衰老中的应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111415058.9A CN114107460A (zh) | 2021-11-25 | 2021-11-25 | 一种基因抑制剂在抑制脐带血间充质干细胞衰老中的应用 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN114107460A true CN114107460A (zh) | 2022-03-01 |
Family
ID=80373284
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202111415058.9A Pending CN114107460A (zh) | 2021-11-25 | 2021-11-25 | 一种基因抑制剂在抑制脐带血间充质干细胞衰老中的应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN114107460A (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114934052A (zh) * | 2022-06-14 | 2022-08-23 | 贵州医科大学附属医院 | 一种长链非编码rna aabr07017227的应用 |
-
2021
- 2021-11-25 CN CN202111415058.9A patent/CN114107460A/zh active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114934052A (zh) * | 2022-06-14 | 2022-08-23 | 贵州医科大学附属医院 | 一种长链非编码rna aabr07017227的应用 |
CN114934052B (zh) * | 2022-06-14 | 2023-05-02 | 贵州医科大学附属医院 | 一种长链非编码rna aabr07017227的应用 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Chen et al. | MicroRNA-130a promotes the metastasis and epithelial-mesenchymal transition of osteosarcoma by targeting PTEN | |
Cai et al. | Identification and analysis of the expression of microRNA from lactating and nonlactating mammary glands of the Chinese swamp buffalo | |
CN111454953B (zh) | 一种骨髓间充质干细胞成脂转化促进剂 | |
CN116355898B (zh) | miRNA-133在调节绵羊胚胎毛囊发育中的应用 | |
Chen et al. | The role of Pin1 protein in aging of human tendon stem/progenitor cells | |
CN107663539B (zh) | 环状RNA circ-PTGR1的用途 | |
CN111235097B (zh) | Linc01877在骨髓间充质干细胞成骨分化中的应用 | |
CN111254146B (zh) | Linc01331基因抑制剂在制备治疗肺癌药物中的用途 | |
CN114196624B (zh) | 促进脐带血间充质干细胞增殖的基因抑制剂 | |
CN114107460A (zh) | 一种基因抑制剂在抑制脐带血间充质干细胞衰老中的应用 | |
Li et al. | Melanoma stem cells promote metastasis via exosomal miR-1268a inactivation of autophagy | |
CN107213161B (zh) | 长链非编码rna rp11-224o19.2抑制剂的用途 | |
CN115851972B (zh) | 一种绵羊毛囊发育标志物miR-23b及其应用 | |
CN112877433B (zh) | 一种结直肠癌靶向治疗药物 | |
CN107881237B (zh) | 肺癌诊断标记物microRNA-4317及在药物和诊断试剂盒中的应用 | |
CN107937523B (zh) | 肺癌诊断标记物microRNA-3607-3p及在药物和诊断试剂盒中的应用 | |
CN107320484B (zh) | mir-452-3p在制备治疗肝癌的药物组合物中的用途 | |
CN113549593B (zh) | 一种促进表皮干细胞增殖的药物制剂 | |
CN112301030B (zh) | 一种抑制Hippo信号通路和胃癌的基因抑制剂 | |
CN113350368B (zh) | 一种基因抑制剂在制备表皮干细胞迁移药物制剂中的应用 | |
CN111118154B (zh) | Linc01272在制备肿瘤检测试剂和/或治疗药物中的应用 | |
CN112280859B (zh) | 一种乳腺癌标志物及应用 | |
CN114317755B (zh) | m6A去甲基化酶FTO在抑制NPM1突变白血病细胞增殖方面的应用 | |
CN113717935B (zh) | Nigroain-E1多肽在维持脂肪干细胞干性中的应用 | |
CN115197944B (zh) | 长链非编码rna mafa-as1在三阴性乳腺癌诊断及治疗中的应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information |
Inventor after: Jiao Sujuan Inventor after: Li Dexia Inventor after: Wang Yingcui Inventor after: Xu Yintao Inventor after: Liu Xiaohua Inventor after: Qin Juan Inventor before: Li Dexia Inventor before: Wang Yingcui Inventor before: Xu Yintao Inventor before: Liu Xiaohua Inventor before: Qin Juan |
|
CB03 | Change of inventor or designer information |