CN114107331A - 参与烟草干旱胁迫反应的基因及其应用 - Google Patents
参与烟草干旱胁迫反应的基因及其应用 Download PDFInfo
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Abstract
本发明公开了一种参与烟草干旱胁迫反应的基因，所述基因的核苷酸序列如SEQ ID NO.1所示。本发明将得到的基因构建到基因编辑的表达载体，将获得的基因编辑的烟草植株进行烟草干旱胁迫反应后其过氧化物酶、脯氨酸含量提高，特别是脯氨酸含量提高明显。
Description
技术领域
本发明属于植物基因工程技术领域，具体涉及参与烟草干旱胁迫反应的基因及其应用。
背景技术
过氧化物酶(Peroxidase,POD)是一类氧化还原酶，广泛存在于细菌、真菌、植物及动物中。目前报道中已有多种植物中的过氧化物酶基因被克隆，如玉米(Yu等虎，2015)，棉花(高正银等，2006)，紫花苜蓿(张怡，2013)等。山楂POD72基因在烟草转基因植物中异位表达，发现其可能参与木质素合成。过量表达POD基因的烟草能增强耐热性(刘飞虎，2006)，增强耐铝毒害性(杨志丽，2015)和盐胁迫能力(Lu，等2020)。目前在一些植物如玉米、棉花和山楂等中POD基因已经被报道相关功能；但烟草POD基因在干旱胁迫中的功能未知。
为解决上述问题提出本发明。
发明内容
本发明从烟草中克隆得到POD基因，如SEQ ID NO.1所示。具体技术方案是参考GenBank收录烟草POD序列，登录号是XP_016440067.1，采用Primer5.0软件设计引物，见下表1。扩增反应程序为：95℃预变性5min；95℃变性30s；55℃退火30s；72℃延伸90s；35个循环。目的片段纯化后与pMD19-T载体16℃连接过夜，连接产物转化大肠杆菌DH5α感受态，随后在涂有氨苄青霉素的LB平板上进行筛选，采用菌落PCR检测阳性克隆。检测后随机选取3个独立的阳性克隆送生物技术公司进行测序，得到的烟草POD基因的核苷酸序列如SEQ IDNO.1所示，该烟草POD基因核苷酸序列全长990bp。随后将该基因构建到基因编辑的表达载体，利用农杆菌介导的方法进行烟草红花大金元遗传转化，利用获得的基因编辑材料进行功能研究。
本发明第一方面公开了一种参与烟草干旱胁迫反应的基因，所述基因的核苷酸序列如SEQ ID NO.1所示，核苷酸序列全长990bp。
优选地，所述参与烟草干旱胁迫反应的基因的编码蛋白。
优选地，所述编码蛋白的氨基酸序列如SEQ ID NO.2所示，包括329个氨基酸。
本发明第二方面公开了所述的参与烟草干旱胁迫反应的基因的应用，将所述基因构建到基因编辑的表达载体，利用农杆菌介导的方法进行烟草遗传转化，然后将获得的基因编辑的烟草植株进行干旱胁迫反应。
优选地，所有植株包括对照烟草植株与基因编辑后的烟草植株干旱胁迫后其过氧化物酶、脯氨酸含量及过氧化氢浓度提高，特别是脯氨酸含量提高明显；但叶绿素含量降低。
优选地，所述基因编辑后的烟草植株干旱胁迫后，其叶绿素含量、过氧化物酶和脯氨酸含量均高于对照烟草植株；但过氧化氢浓度略低于对照烟草植株。
本发明的有益效果：
1、本发明通过同源克隆从烟草中得到POD基因，其核苷酸序列如SEQ ID NO.1所示，核苷酸序列全长990bp碱基。
2、本发明将得到的基因构建到基因编辑的表达载体，利用农杆菌介导的方法进行烟草遗传转化，然后将获得的基因编辑的烟草植株进行烟草干旱胁迫反应后其过氧化物酶、脯氨酸含量提高，特别是脯氨酸含量提高明显。
附图说明
图1为本发明的POD基因的电泳图。
图2为POD基因编辑的烟草植株干旱处理表型检测；HD为对照样即普通烟草植株，T1-T3为基因编辑后的烟草植株；其中左边为正常生长，右边为干旱处理后。
图3为POD基因编辑的烟草植株干旱处理后叶绿素含量测定；HD为对照样即普通烟草植株，T1-T3为基因编辑后的烟草植株；其中左边为正常生长，右边为干旱处理。
图4为POD基因编辑的烟草植株干旱处理后过氧化物酶(POD)含量测定；HD为对照样即普通烟草植株，T1-T3为基因编辑后的烟草植株；其中左边为正常生长，右边为干旱处理。
图5为POD基因编辑的烟草植株干旱处理后过脯氨酸含量测定；HD为对照样即普通烟草植株，T1-T3为基因编辑后的烟草植株；其中左边为正常生长，右边为干旱处理。
图6为POD基因编辑的烟草植株干旱处理后过氧化氢浓度测定；HD为对照样即普通烟草植株，T1-T3为基因编辑后的烟草植株；其中左边为正常生长，右边为干旱处理。
具体实施方式
以下通过实施例来详细说明本发明的技术方案，以下的实施例仅是示例性的，仅能用来解释和说明本发明的技术方案，而不能解释为是对本发明技术方案的限制。在本申请的各实施例中，没有注明具体技术或条件者，按照本领域内现有技术或条件进行，所使用的材料或设备未注明生产厂商者，均为可以通过购买获得的常规产品。本发明除非另有说明，否则百分号为体积百分数，比例为体积比。
实施例1：基因克隆及烟草植株基因编辑
1、基因克隆
取0.5g烟草新鲜叶片，采用Trizol法提取烟草细胞的总RNA，然后采用TaKaRa公司的cDNA合成试剂盒合成cDNA，进一步采用Primer5.0软件设计引物对cDNA进行PCR扩增，引物见下表1。
扩增反应程序为：95℃预变性5min；95℃变性30s；55℃退火30s；72℃延伸90s；35个循环。目的片段纯化后与pMD19-T载体16℃连接过夜，连接产物转化大肠杆菌DH5α感受态，随后在涂有氨苄青霉素的LB平板上进行筛选，采用菌落PCR检测阳性克隆。检测后随机选取3个独立的阳性克隆送生物技术公司进行测序，得到的烟草POD基因的核苷酸序列如SEQ ID NO.1所示，该烟草POD72基因核苷酸序列全长990bp碱基。
表1引物列表
图1为得到的POD基因的电泳图。
2、目的基因连接表达载体
将上述转化好的T-载体与基因编辑的表达载体进行双酶切，回收目的基因和表达载体，然后用连接酶连接，将连接后的重组表达载体转入大肠杆菌DH5α的感受态细胞，对转化后的大肠杆菌单菌落进行PCR扩增，然后挑取阳性菌落扩繁、提取质粒进行酶切和测序来检测是否构建成功。
3、冻融法转化农杆菌和PCR检测
取200μL感受态细胞，室温融化后加入10μL构建好的重组质粒DNA，混匀后冰浴30min，液氮中速冻1min，37℃水浴5min，然后加入500μL含利福平和链霉素的LB培养基，28℃慢速振荡培养4h，10000r/min离心1min集菌，弃上清，加入500μL含利福平和链霉素的LB培养基重新悬浮细胞，涂布于含有50μg/mL利福平、50μg/mL链霉素和50μg/mL卡那霉素的平板上，28℃避光培养约48h。待平板上长出的单菌落，直接用灭菌的牙签蘸取单菌落，在PCR体系中晃动几下后进行PCR扩增反应。
PCR体系为：
反应体系为：
1.0％的琼脂糖凝胶电泳检测PCR产物结果：若空白对照没有条带，但转录PCR产物有明亮且大小正确的条带，证明转化成功。
4、农杆菌转化烟草方法
4.1烟草无菌苗的培养和预培养
选取籽粒饱满无病虫害的烟草种子装入1.5mL EP管中先用70％的乙醇消毒30s，无菌水冲洗3-5次，然后用1mL 30％次氯酸钠溶液消毒5min，吸出消毒液，再加入1mL30％次氯酸钠溶液消毒25min，消毒期间都要不断地振荡EP管。最后用无菌水反复清洗6-7次后，吸干种子表面水分，布于MS培养基上，于光照培养箱最大光强度、温度20℃、光照16h/d培养30d左右备用。选取约30d苗龄且长势良好的烟草无菌苗，用灭菌刀片将烟草叶片切下，放置在预培养基上培养2d(注意培养基要倒置，即叶的正面朝下)。
4.2侵染菌液的制备
将含有目的基因的农杆菌在固体LB培养基上划板，28℃下暗培养2d。用灭菌牙签挑取菌落，接种于2mL液体LB培养基中，28℃下振荡培养过夜(12h左右)。活化过夜的农杆菌，按1:50的比例，取100μL稀释到5mL LB培养基中，继续培养至OD600值为0.5(约3h检测一次)。取培养物1mL置于无菌离心管中，12000r/min离心1min，弃上清。加入100mL的MS0培养基，混匀备用。
4.3侵染叶片、共培养和分化培养
将在预培养基上培养2d的烟草叶片切成1cm2左右的叶盘，置于悬菌液中(MS0悬浮，可以稀释50-100倍)浸泡3-5min。然后取出，用无菌滤纸吸去其表面的液体。将侵染过的叶盘分别接种在覆有一层滤纸的共培养基上面，放到恒温培养箱20℃暗培养2d。用100mL含有200μL头孢霉素，100μL羧苄青霉素的无菌水清洗4min，重复一次后再用无菌水清洗8min，最后用无菌滤纸吸去其表面的液体再转入分化培养基进行分化培养，前期3d继代一次，每次继代需在无菌条件下进行，连续继代3次后就每隔两周继代一次。
4.4生根培养和繁殖
待抗性芽长至1-2cm时，在超净台上切去芽基部的所有愈伤组织及基部叶片，植于生根培养基上。待根长至2-3cm时，取出无菌苗，轻轻打碎固体培养基，洗去残留的培养基，去掉下部叶，然后将无菌苗植入土壤，室内培养大约一周后移到室外(最初的3d应在阴暗处生长且要盖上透明塑料)。经过PCR检测为阳性的烟草植株进行繁殖到T3代。
实施例2：基因编辑后的烟草植株干旱胁迫实验
1、基因编辑后的烟草植株干旱处理后表型观察
采用漂浮育苗的方法，对三个基因编辑后的烟草植株和对照样(普通烟草植株)进行培养，30天后种植在培养盘中进行干旱胁迫实验；干旱处理15天后进行照相和生理指标的测定，结果如图2所示。从图2可以明显看出干旱处理15天后的三个基因编辑后的烟草植株明显长势优于对照样。
2、基因编辑后的烟草植株干旱处理后的生理指标测定
对三个基因编辑后的烟草植株进行干旱处理后生理指标进行测定，并与对照样(普通烟草植株)进行干旱处理后比较，结果如图3-图6所示。由图3-图6可以看出，叶绿素含量、过氧化物酶和脯氨酸含量在干旱处理15天后基因编辑后的烟草植株中明显高于对照样；而在正常的生长条件下，基因编辑后的烟草植株与对照样没有明显差异，见图3-图5。而过氧化氢含量测定结果表明在干旱处理15天，后基因编辑后的烟草植株中过氧化氢含量明显低于对照样；而在正常的生长条件下基因编辑后的烟草植株与对照样没有明显差异，如图6所示。
以上结果表明，烟草POD基因编辑后的烟草植株具有较高抵抗干旱胁迫的能力。
以上显示和描述了本发明的基本原理、主要特征和本发明的优点。本行业的技术人员应该了解，本发明不受上述实施例的限制，上述实施例和说明书中描述的只是说明本发明的原理，在不脱离本发明精神和范围的前提下，本发明还会有各种变化和改进，这些变化和改进都落入要求保护的本发明范围内。本发明要求保护范围由所附的权利要求书及其等效物界定。
序列表
<110> 云南中烟工业有限责任公司
<120> 参与烟草干旱胁迫反应的基因及其应用
<130> RIB210606
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 990
<212> DNA
<213> 人工序列(POD)
<400> 1
atggctataa aaatgcttct ttttctatgc atttccgcct tattaagtac ttcctcttta 60
tcccagcgcc attcgccgct aaatactgct ttttaccgca aatcatgccc aagatttgag 120
cagataatgg aagaaaccac tacaaacaaa cagatcactt ctcctactac cgccgccgcc 180
accctccgcc tcttcttcca cgactgcttc gtcggcggct gtgacgcctc cgtacttgtc 240
tcctccactc ccttcaacaa agccgagcgt gacgccgaaa tcaacctttc cctccccggc 300
gatggcttcg acgtggtcgt acgcgccaag accgcgcttg aactttcctg tccaggtata 360
gtctcttgct ccgacattct cgccgtcgct gcccgaaacc ttgtcgtcca aactggcggc 420
ccgttttatc cagttaaatt gggccgtaaa gattcttttg tatcgaaagc ctcattagta 480
gaaggaaatc tgccccggcc cacaatgccg atggatcaaa tcattaagat tttcgaatcc 540
agaggatttt cgattcaaga aatggtagca ttatctggag ctcacacaat tggattttcc 600
cactgtaaag agttcaactc ggatctttat aactacaaca aaacttcaca agctgatcct 660
tcgtataatc cgaggtttgc tcaagctttg aaagatgctt gtagtaattc ccagaaagat 720
ccaacattat cagtgttcaa cgatataatg actcctaaca aattcgataa tatgtattat 780
cagaacttgc ctaagggttt gggtttattg tcttcagacc gtggtctgtt ttcagatccg 840
aggacgagaa ttcacgttga ggaatatgtt agagatcaga atgcattttt taaggcgttt 900
gcttcagcaa tgcagaagct tagtgaacat ggtgttaaaa ttggtaaaca tggtgagatc 960
aggcacaggt gtgacgcttt caacaattga 990
<210> 2
<211> 329
<212> PRT
<213> 人工序列(POD)
<400> 2
Met Ala Ile Lys Met Leu Leu Phe Leu Cys Ile Ser Ala Leu Leu Ser
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Thr Ser Ser Leu Ser Gln Arg His Ser Pro Leu Asn Thr Ala Phe Tyr
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Arg Lys Ser Cys Pro Arg Phe Glu Gln Ile Met Glu Glu Thr Thr Thr
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Asn Lys Gln Ile Thr Ser Pro Thr Thr Ala Ala Ala Thr Leu Arg Leu
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Phe Phe His Asp Cys Phe Val Gly Gly Cys Asp Ala Ser Val Leu Val
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Ser Ser Thr Pro Phe Asn Lys Ala Glu Arg Asp Ala Glu Ile Asn Leu
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Ser Leu Pro Gly Asp Gly Phe Asp Val Val Val Arg Ala Lys Thr Ala
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Leu Glu Leu Ser Cys Pro Gly Ile Val Ser Cys Ser Asp Ile Leu Ala
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Val Ala Ala Arg Asn Leu Val Val Gln Thr Gly Gly Pro Phe Tyr Pro
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Val Lys Leu Gly Arg Lys Asp Ser Phe Val Ser Lys Ala Ser Leu Val
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Glu Gly Asn Leu Pro Arg Pro Thr Met Pro Met Asp Gln Ile Ile Lys
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Ile Phe Glu Ser Arg Gly Phe Ser Ile Gln Glu Met Val Ala Leu Ser
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Gly Ala His Thr Ile Gly Phe Ser His Cys Lys Glu Phe Asn Ser Asp
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Leu Tyr Asn Tyr Asn Lys Thr Ser Gln Ala Asp Pro Ser Tyr Asn Pro
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Arg Phe Ala Gln Ala Leu Lys Asp Ala Cys Ser Asn Ser Gln Lys Asp
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Pro Thr Leu Ser Val Phe Asn Asp Ile Met Thr Pro Asn Lys Phe Asp
245 250 255
Asn Met Tyr Tyr Gln Asn Leu Pro Lys Gly Leu Gly Leu Leu Ser Ser
260 265 270
Asp Arg Gly Leu Phe Ser Asp Pro Arg Thr Arg Ile His Val Glu Glu
275 280 285
Tyr Val Arg Asp Gln Asn Ala Phe Phe Lys Ala Phe Ala Ser Ala Met
290 295 300
Gln Lys Leu Ser Glu His Gly Val Lys Ile Gly Lys His Gly Glu Ile
305 310 315 320
Arg His Arg Cys Asp Ala Phe Asn Asn
325
Claims (6)
1.一种参与烟草干旱胁迫反应的基因，其特征在于，所述基因的核苷酸序列如SEQ IDNO.1所示。
2.根据权利要求1所述的参与烟草干旱胁迫反应的基因，其特征在于，所述基因的编码蛋白。
3.根据权利要求2所述的参与烟草干旱胁迫反应的基因，其特征在于，所述编码蛋白的氨基酸序列如SEQ ID NO.2所示。
4.根据权利要求1-3任一所述的参与烟草干旱胁迫反应的基因的应用，其特征在于，将所述基因构建到基因编辑的表达载体，利用农杆菌介导的方法进行烟草遗传转化，然后将获得的基因编辑的烟草植株进行干旱胁迫反应。
5.根据权利要求4所述的应用，其特征在于，对照烟草植株与基因编辑后的烟草植株干旱胁迫后其过氧化物酶含量、脯氨酸含量及过氧化氢浓度提高。
6.根据权利要求4所述的应用，其特征在于，所述基因编辑后的烟草植株干旱胁迫后，其叶绿素含量、过氧化物酶和脯氨酸含量均高于对照烟草植株。
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140026254A1 (en) * | 2012-07-17 | 2014-01-23 | Industry Foundation Of Chonnam National University | Gene Implicated in Drought Stress Tolerance and Transformed Plants with the Same |
| CN108795894A (zh) * | 2018-06-25 | 2018-11-13 | 四川农业大学 | 烟草pod63蛋白、编码基因及其在烟草盐胁迫反应中的应用 |
-
2021
- 2021-12-02 CN CN202111461484.6A patent/CN114107331A/zh active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140026254A1 (en) * | 2012-07-17 | 2014-01-23 | Industry Foundation Of Chonnam National University | Gene Implicated in Drought Stress Tolerance and Transformed Plants with the Same |
| CN108795894A (zh) * | 2018-06-25 | 2018-11-13 | 四川农业大学 | 烟草pod63蛋白、编码基因及其在烟草盐胁迫反应中的应用 |
Non-Patent Citations (1)
| Title |
|---|
| 刘国顺主编: "烤烟旱作高产栽培技术", vol. 1, 31 May 2015, 北京理工大学出版社, pages: 85 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113046371A (zh) * | 2021-03-22 | 2021-06-29 | 云南中烟工业有限责任公司 | 一种烟草过氧化物酶相关的基因及其应用 |
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