CN114100715A - Sample injection mode for digital micro-fluidic chip - Google Patents
Sample injection mode for digital micro-fluidic chip Download PDFInfo
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- CN114100715A CN114100715A CN202111386783.8A CN202111386783A CN114100715A CN 114100715 A CN114100715 A CN 114100715A CN 202111386783 A CN202111386783 A CN 202111386783A CN 114100715 A CN114100715 A CN 114100715A
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- Prior art keywords
- sample injection
- pressure head
- shaft sleeve
- microfluidic chip
- driving
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
- B01L3/0293—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
Abstract
The invention discloses a sample injection method and a sample injection device for a digital microfluidic chip. The sample injection device of the digital microfluidic chip mainly comprises a sample injection pressure head (1) and a driving mechanism (6) for driving the sample injection pressure head to move. The sample injection pressure head (1) is used for injecting a reagent pre-embedded in the chip (12) into the chip (12). The driving mechanism (6) is used for controlling the movement of the sample injection pressure head (1) to realize automatic sample injection. The sample injection method has the advantages of high sensitivity, high accuracy, high flux, high integration degree and the like, and the whole-flow reaction has no cross contamination, can be operated by one key, and greatly liberates the hands of operators.
Description
Technical Field
The invention relates to a sample injection method and a sample injection device for a digital microfluidic chip.
Background
At present, a digital microfluidic chip needs to be used in cooperation with a detection reagent to have a certain specific detection function, and in the prior art, a liquid transfer gun is generally used for absorbing a certain amount of liquid reagent samples, and then the liquid reagent samples are aligned to a sample inlet of the chip, so that the reagent is completely injected into the chip. However, the use of a pipette to inject a reagent increases the use cost, is complicated in process and has strong dependence on the reagent, and the traditional sample injection method cannot realize the pre-embedding of the detection reagent on the digital microfluidic chip, so that the use environment and the sample injection condition of the digital microfluidic chip are greatly limited.
At present, centrifugal force driving or air pressure driving is mostly adopted for micro-fluidic chips which adopt automatic sample injection in the market. Centrifugal force drive has higher requirements on chip manufacturing, the micro-valve effect needs to be realized through inner diameter mutation, air pressure balance of various channels needs to be ensured during rotation, and consistency of the chip is difficult to control. The air pressure driving type air source system needs an external air source and a plurality of control valves, has extremely high requirement on air circuit tightness, is complex in overall control and has higher cost. The existing automatic sample injection method has the defects of high processing and manufacturing difficulty, high cost, complex sample injection process and difficult assembly. In mass production, the sample injection consistency of each chip is difficult to ensure, which is not beneficial to mass production.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to quantitatively seal a reagent and other substances (related liquid, solid or solid-liquid mixed liquid and the like) required for detection in a reagent package in advance, pre-embed the reagent package in a micro-fluidic chip, and compress the reagent package by a sample injection method designed by the invention so as to inject the reagent broken by the reagent package into a chip cavity.
The invention provides a sample injection device of a digital microfluidic chip, which mainly comprises a sample injection pressure head 1 and a driving mechanism 6 for driving the sample injection pressure head to move. The sample injection pressure head 1 is used for injecting the reagent pre-embedded in the chip 12 into the chip 12. The driving mechanism 6 is used for controlling the movement of the sample injection pressure head 1 to realize automatic sample injection.
Annotate appearance pressure head 1 by at least one pressure head axle sleeve 2, stopper beat screw 3, compression spring 4 and annotate appearance clamp plate 5 and constitute. The sample injection pressing plate 5 is provided with a mounting hole for fixing the plug screw 3. Compression spring 4 assembles between notes appearance clamp plate 5 and pressure head axle sleeve 2, has the echelonment fixed orifices in the pressure head axle sleeve 2, the fixed orifices is along being close to annotating 5 direction internal diameters of appearance clamp plate and reducing, and 3 slip of stopper screw pass pressure head axle sleeve 2 internal fixation holes, and 3 nut inboards and the fixed orifices ladder terminal surface butt of stopper screw are beated to the stopper, with pressure head axle sleeve 2 activity assembly on annotating appearance clamp plate 5. The pressure head shaft sleeve 2 is used for directly contacting the reagent pack and compressing the reagent pack to generate deformation or directly crushing the reagent pack;
preferably, the pressure head shaft sleeve 2 is provided with an initial working position and a pressing position, when the pressure head shaft sleeve 2 is at the initial working position, the compression spring 4 pushes against the pressure head shaft sleeve 2, and when the pressure head shaft sleeve 2 moves to the pressing position, the compression spring 4 generates elastic deformation and is compressed;
preferably, the number of the pressure head shaft sleeves 2 corresponds to the number of the reagent packs in the microfluidic chip 12 one by one;
preferably, the number of the sample injection heads 1 can be increased in parallel according to the number of the microfluidic chips 12.
The driving mechanism 6 mainly comprises a base 7, a driving plate 8, a driving motor 9, a sliding rail 10 and a supporting plate 11. The driving motor 9 and the sliding rail 10 are installed in parallel in the same plane, the driving plate 8 is assembled on the sliding rail 10 and the driving motor 9, the driving motor 9 drives the driving plate 8 to move, and the supporting plate 11 is used for assembling the sample injection pressure head 1 and the driving mechanism 6 together.
Preferably, the sample injection pressure head 1 is vertically installed with the driving plate 8 through the supporting plate 11, and the driving motor 9 drives the driving plate 8 to drive the sample injection pressure head 1 to move.
Preferably, more than two driving mechanisms 6 for driving the sample injection pressure head to move can be arranged in parallel according to the number of the chips 12.
The invention provides a sample injection method of a digital microfluidic chip. The driving motor 9 controls the movement of the sample injection pressure head 1, the pressure head shaft sleeve 2 assembled in the sample injection pressure head 1 has an initial working position and a compression position when moving, the sample injection pressure head 1 is driven by the driving motor 9 to move towards the direction of a reagent package sealed in the microfluidic chip when the pressure head shaft sleeve 2 is at the initial working position until the pressure head shaft sleeve 2 contacts the reagent package and compresses the reagent package to break the reagent package, the reagent in the reagent package is injected into the chip, and the compression spring 4 assembled with the pressure head shaft sleeve 2 pushes against the pressure head shaft sleeve 2 at the moment. The sample injection pressure head 1 continues to be driven to move until the pressure head shaft sleeve 2 moves to a compression position, the compression spring 4 assembled with the pressure head shaft sleeve 2 deforms and is compressed, and the reagent in the reagent pack is completely injected into the chip.
Compared with the prior art, the invention has the following beneficial effects:
(1) the sample injection is carried out without using a pipette gun, and only the reagent is pre-embedded in the chip in advance, so that the automatic sample injection of the reagent can be realized through the sample injection mode;
(2) inconvenience and waste caused by manual misoperation can be effectively prevented, the detection efficiency is greatly improved, and the detection cost is reduced;
(3) for the micro-fluidic chip, a complex micro-channel and air circuit structure does not need to be designed, a pump and a valve are not needed to be controlled by the whole machine, an internal air pipe is not needed to be connected, and the assembling reliability of the whole machine is high;
(4) the sample injection precision is high, all reagent packages can be completely injected into the microfluidic chip, less sample consumption is realized, the advantages of high sensitivity, high precision, high flux, high integration degree and the like are achieved, cross contamination is avoided in the whole-process reaction, one-key operation can be realized, and the hands of operators are greatly liberated.
Drawings
Figures 1-a and 1-B show a front view and a cross-sectional view, respectively, of the injection ram 1;
fig. 2 shows a front view of the drive mechanism 6;
fig. 3 shows a front view of the assembly of the sample injection ram 1 and the drive mechanism 6;
fig. 4 shows the position relationship between the sample injection device and the microfluidic chip 12, at least one reagent pack is arranged in the microfluidic chip 12, and each reagent pack corresponds to the pressure head shaft sleeve 2 one by one;
fig. 5-a and 5-B show a front view and a cross-sectional view, respectively, of the indenter sleeve 2 fully injecting the reagent pack in the microfluidic chip 12 into the chip.
Detailed Description
To further illustrate the technical means adopted by the present invention and the effects thereof, the present invention is further described below with reference to the embodiments and the accompanying drawings. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting of the invention.
The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications. The reagents or apparatus used are conventional products commercially available from normal sources, not indicated by the manufacturer.
Example 1: sample injection device structure of digital microfluidic chip
The sample injection device of the digital microfluidic chip mainly comprises a sample injection pressure head 1 and a driving mechanism 6 for driving the sample injection pressure head to move. The sample injection pressure head 1 is used for injecting the reagent pre-embedded in the chip 12 into the chip 12. The driving mechanism 6 is used for controlling the movement of the sample injection pressure head 1 to realize automatic sample injection.
Fig. 1-a and fig. 1-B show a front view and a cross-sectional view of a sample injection press head 1, respectively, the sample injection press head 1 being composed of a plurality of press head shaft sleeves 2, a tucking screw 3, a compression spring 4, and a sample injection press plate 5. Compression spring 4 assembles between notes appearance clamp plate 5 and pressure head axle sleeve 2, has the echelonment fixed orifices in the pressure head axle sleeve 2, the fixed orifices is along being close to annotating 5 direction internal diameters of appearance clamp plate and reducing, and 3 slip of stopper screw pass pressure head axle sleeve 2 internal fixation holes, and 3 nut inboards and the fixed orifices ladder terminal surface butt of stopper screw are beated to the stopper, with pressure head axle sleeve 2 activity assembly on annotating appearance clamp plate 5. The pressure head shaft sleeve 2 is used for directly contacting the reagent pack and compressing the reagent pack to generate deformation or directly crushing the reagent pack;
the pressure head shaft sleeve 2 is provided with an initial working position and a pressing position, when the pressure head shaft sleeve 2 is at the initial working position, the compression spring 4 pushes against the pressure head shaft sleeve 2, and when the pressure head shaft sleeve 2 moves to the pressing position, the compression spring 4 generates elastic deformation and is compressed;
the number of the pressure head shaft sleeves 2 corresponds to the number of the reagent packs in the microfluidic chip 12 one by one;
the number of the sample injection pressure heads 1 can be increased in parallel according to the number of the microfluidic chips 12.
Fig. 2 shows a front view of the driving mechanism 6, and the driving mechanism 6 mainly comprises a base 7, a driving plate 8, a driving motor 9, a sliding rail 10 and a supporting plate 11. The driving motor 9 and the sliding rail 10 are installed in parallel in the same plane, the driving plate 8 is assembled on the sliding rail 10 and the driving motor 9, the driving motor 9 drives the driving plate 8 to move, and the supporting plate 11 is used for assembling the sample injection pressure head 1 and the driving mechanism 6 together.
Annotate appearance pressure head 1 and pass through backup pad 11 and drive plate 8 vertical installation together, driving motor 9 drives the activity of the drive plate 8 drive notes appearance pressure head 1.
Fig. 3 shows a front view of the assembly of the injection head 1 and the drive mechanism 6.
Fig. 4 shows the position relationship between the sample injection device and the microfluidic chip 12, and at least one reagent pack is arranged in the microfluidic chip 12, and each reagent pack corresponds to the pressure head shaft sleeve 2 one by one.
More than two driving mechanisms 6 for driving the sample injection pressure head to move can be arranged in parallel according to the number of the chips 12.
Example 2: sample injection method of digital microfluidic chip
Fig. 5-a and 5-B show a front view and a cross-sectional view, respectively, of the indenter sleeve 2 fully injecting the reagent pack in the microfluidic chip 12 into the chip. The driving motor 9 controls the movement of the sample injection pressure head 1, the pressure head shaft sleeve 2 assembled in the sample injection pressure head 1 has an initial working position and a compression position when moving, the sample injection pressure head 1 is driven by the driving motor 9 to move towards the direction of a reagent package sealed in the microfluidic chip when the pressure head shaft sleeve 2 is at the initial working position until the pressure head shaft sleeve 2 contacts the reagent package and compresses the reagent package to break the reagent package, the reagent in the reagent package is injected into the chip, and the compression spring 4 assembled with the pressure head shaft sleeve 2 pushes against the pressure head shaft sleeve 2 at the moment. The sample injection pressure head 1 continues to be driven to move until the pressure head shaft sleeve 2 moves to a compression position, the compression spring 4 assembled with the pressure head shaft sleeve 2 deforms and is compressed, and the reagent in the reagent pack is completely injected into the chip.
The applicant asserts that the foregoing is merely a preferred embodiment of the present invention and the technical principles employed. It will be understood by those skilled in the art that the present invention is not limited to the particular embodiments described herein, but is capable of various obvious changes, rearrangements and substitutions as will now become apparent to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail by the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the spirit of the present invention, and the scope of the present invention is determined by the scope of the appended claims.
Claims (9)
1. A sample injection device for a digital microfluidic chip is characterized by comprising a sample injection pressure head (1) and a driving mechanism (6) for driving the sample injection pressure head to move; wherein the content of the first and second substances,
the sample injection pressure head (1) comprises at least one pressure head shaft sleeve (2), a plugging screw (3), a compression spring (4) and a sample injection pressure plate (5);
the driving mechanism (6) comprises a base (7), a driving plate (8), a driving motor (9), a sliding rail (10) and a supporting plate (11).
2. The sample injection device of the digital microfluidic chip according to claim 1, wherein the sample injection pressure plate (5) is provided with a mounting hole for fixing the plug screw (3), the pressure head shaft sleeve (2) is provided with a fixing hole therein, the compression spring (4) is assembled between the sample injection pressure plate (5) and the pressure head shaft sleeve (2), and the plug screw (3) slides through the fixing hole in the pressure head shaft sleeve (2) to movably assemble the pressure head shaft sleeve (2) on the sample injection pressure plate (5).
3. The sample injection device of the digital microfluidic chip according to claim 2, wherein the indenter shaft sleeve (2) has an initial working position and a pressing position, when the indenter shaft sleeve (2) is in the initial working position, the compression spring (4) pushes against the indenter shaft sleeve (2), and when the indenter shaft sleeve (2) moves to the pressing position, the compression spring (4) generates elastic deformation and is compressed;
preferably, the compression shaft sleeve (2) internal fixation hole is the echelonment through-hole, and the fixed orifices is along being close to notes appearance clamp plate (5) direction internal diameter and reducing, stopper is beaten screw (3) and is passed pressure head shaft sleeve (2) fixed orifices, stopper is beaten screw (3) nut inboard and fixed orifices ladder terminal surface butt.
4. The sample injection device of the digital microfluidic chip according to any one of claims 1 to 3, wherein the driving plate (8) is mounted on a slide rail (10) and a driving motor (9), the driving motor (9) drives the driving plate (8) to move, and the supporting plate (11) is used for mounting the sample injection head (1) and the driving mechanism (6) together.
5. The sample injection device of the digital microfluidic chip according to claim 4, wherein the sample injection head (1) is vertically installed with the driving plate (8) through the supporting plate (11), and the driving motor (9) drives the driving plate (8) to drive the sample injection head (1) to move.
6. The sample injection device of the digital microfluidic chip according to any one of claims 1 to 5, wherein the sample injection pressure head (1) is used for injecting a reagent pre-embedded in the microfluidic chip (12) into the microfluidic chip (12), and the number of the pressure head shaft sleeves (2) corresponds to the number of the reagent packets in the microfluidic chip (12) one by one;
preferably, the number of the sample injection pressure heads (1) can be increased in parallel according to the number of the microfluidic chips (12).
7. The sample injection device of the digital microfluidic chip according to claim 6, wherein more than two driving mechanisms (6) are arranged in parallel according to the number of the microfluidic chips (12).
8. A sample injection method of a digital microfluidic chip is characterized in that the sample injection device of the digital microfluidic chip is used for injecting samples into the digital microfluidic chip according to any one of claims 1 to 7.
9. The method according to claim 8, comprising in particular: the sample injection pressure head (1) is controlled to move by the movement of the driving motor (9), the pressure head shaft sleeve (2) assembled in the sample injection pressure head (1) is provided with an initial working position and a compression position, the sample injection pressure head (1) is driven by the driving motor (9) to move towards a reagent pack sealed in the microfluidic chip when the pressure head shaft sleeve (2) is at the initial working position until the pressure head shaft sleeve (2) contacts the reagent pack and compresses the reagent pack to break the reagent pack, the reagent in the reagent pack is injected into the chip, the compression spring (4) assembled with the pressure head shaft sleeve (2) pushes the pressure head shaft sleeve (2) against the pressure head shaft sleeve at the moment, the sample injection pressure head (1) is continuously driven to move until the pressure head shaft sleeve (2) moves to the compression position, the compression spring (4) assembled with the pressure head shaft sleeve (2) deforms and is compressed at the moment, and the reagent in the reagent pack is completely injected into the microfluidic chip (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111386783.8A CN114100715A (en) | 2021-11-22 | 2021-11-22 | Sample injection mode for digital micro-fluidic chip |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111386783.8A CN114100715A (en) | 2021-11-22 | 2021-11-22 | Sample injection mode for digital micro-fluidic chip |
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| CN114100715A true CN114100715A (en) | 2022-03-01 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202111386783.8A Pending CN114100715A (en) | 2021-11-22 | 2021-11-22 | Sample injection mode for digital micro-fluidic chip |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115078363A (en) * | 2022-08-18 | 2022-09-20 | 广东奥素液芯微纳科技有限公司 | Microfluidic liquid-transfering observation instrument |
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| CN113300190A (en) * | 2021-04-30 | 2021-08-24 | 东莞联鹏智能装备有限公司 | Pressure head leveling device |
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2021
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| CN106434282A (en) * | 2016-10-27 | 2017-02-22 | 杭州安杰思生物科技有限公司 | Micro-fluidic vacuole extrusion mechanism |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115078363A (en) * | 2022-08-18 | 2022-09-20 | 广东奥素液芯微纳科技有限公司 | Microfluidic liquid-transfering observation instrument |
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