CN216093736U - Detection device - Google Patents
Detection device Download PDFInfo
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- CN216093736U CN216093736U CN202122528002.6U CN202122528002U CN216093736U CN 216093736 U CN216093736 U CN 216093736U CN 202122528002 U CN202122528002 U CN 202122528002U CN 216093736 U CN216093736 U CN 216093736U
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Abstract
The utility model discloses a detection device, which comprises a microfluidic reaction component, wherein the microfluidic reaction component comprises a mounting seat, the upward end of the mounting seat is provided with a mounting groove, the downward end of the mounting seat is provided with a first detection port communicated with the mounting groove, and a microfluidic reaction chip for detecting the concentration of a marker is mounted on the mounting seat through the mounting groove; the detector comprises a detection shell, wherein an image collector is arranged in the detection shell, a movable plate capable of moving in the height direction is connected in the detection shell, a mounting seat is placed on the upper side of the movable plate, a second detection port corresponding to the first detection port is formed in the movable plate, and the image collector is arranged right below the second detection port; the utility model has high integration level and is convenient to carry.
Description
Technical Field
The utility model relates to the technical field of microfluidics, in particular to a detection device.
Background
The microfluidic detection technology refers to a technology related to a system for processing or manipulating microfluid by using a channel of several tens to several hundreds of micrometers, and the chemiluminescence immunoassay is a detection and analysis technology for various antigens, haptens, antibodies, hormones, enzymes, drugs and the like by combining a chemiluminescence assay technology with high sensitivity and high specificity immunoreaction.
In the prior art, each device for detecting the concentration of the marker is dispersed and inconvenient to carry, and when an image collector or a heating plate and other devices are needed, the image collector is placed below the microfluidic reaction chip, so that the operation is inconvenient.
SUMMERY OF THE UTILITY MODEL
This section is for the purpose of summarizing some aspects of embodiments of the utility model and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section as well as in the abstract of the specification and the title of the application to avoid obscuring the purpose of this section, the abstract of the specification and the title of the application, and such simplifications or omissions are not intended to limit the scope of the utility model.
The present invention has been made in view of the above and/or other problems with existing microfluidic assays.
Therefore, the utility model aims to provide a detection device which is simple in structure, high in integration level and convenient to carry.
In order to solve the technical problems, the utility model provides the following technical scheme: a detection device, which comprises a detection device,
the microfluidic reaction assembly comprises a mounting seat, wherein a mounting groove is formed in the upward end of the mounting seat, a first detection port communicated with the mounting groove is formed in the downward end of the mounting seat, and a microfluidic reaction chip for detecting the concentration of a marker is mounted on the mounting seat through the mounting groove;
the detector comprises a detection shell, an image collector is installed in the detection shell, a movable plate capable of moving in the height direction is connected in the detection shell, a mounting seat is placed on the upper side of the movable plate, a second detection port corresponding to the first detection port is formed in the movable plate, and the image collector is arranged under the second detection port.
As a preferable aspect of the detection device of the present invention, wherein: the micro-fluidic reaction chip comprises a first chip body, a second chip body and a third chip body which are sequentially connected from top to bottom, wherein a plurality of groups of liquid inlet components are distributed on the first chip body, each liquid inlet component comprises a plurality of liquid storage tanks arranged in the first chip body and a liquid outlet channel connected with the liquid storage tanks, a plurality of liquid inlet holes which are in one-to-one correspondence with the liquid storage tanks and communicated with the liquid storage tanks are distributed at one upward end of the first chip body, a liquid outlet reaction tank is arranged at one downward end of the first chip body, a liquid outlet valve sleeve is connected on the first chip body between the liquid outlet channel and the liquid outlet reaction tank, one end of the liquid outlet channel, far away from the liquid storage tanks, can be communicated with the liquid outlet reaction tank through the liquid outlet valve sleeve, a plurality of reaction counter bores are distributed at one upward end of the second chip body, the liquid outlet reaction tank covers the corresponding reaction counter bores, and a plurality of waste liquid outlet holes which are in one-to one correspondence with the reaction counter bores are also formed on the second chip body, the waste liquid hole is far away from the liquid outlet valve sleeve, and the upward end of the third chip body is provided with a waste liquid pool communicated with the waste liquid hole.
As a preferable aspect of the detection device of the present invention, wherein: a plurality of liquid outlet holes which are in one-to-one correspondence with the liquid outlet channels and communicated with the liquid outlet channels are arranged on the liquid outlet valve sleeve, and liquid outlets which are arranged towards the liquid outlet reaction tank are arranged at one end, far away from the liquid outlet channels, of the liquid outlet valve sleeve.
As a preferable aspect of the detection device of the present invention, wherein: the first chip body is rotatably connected with a liquid outlet control valve body through a liquid outlet valve sleeve, a control liquid hole capable of being communicated with the liquid outlet hole is formed in the liquid outlet control valve body, and when the control liquid hole is communicated with the liquid outlet hole, liquid in the corresponding liquid storage tank sequentially passes through the liquid outlet channel, the liquid outlet hole, the control liquid hole and the liquid outlet control valve body and then enters an area where the liquid outlet reaction tank is located.
As a preferable aspect of the detection device of the present invention, wherein: the upper end of the liquid outlet control valve body is abutted against the upper side of the first chip body.
As a preferable aspect of the detection device of the present invention, wherein: and a non-circular transmission groove is formed in the upward end of the liquid outlet control valve body.
As a preferable aspect of the detection device of the present invention, wherein: the upper end of the detection shell is rotatably connected with a closed door body, one side of the closed door body, which is opposite to the detection shell, is connected with a plurality of transmission motors which are in one-to-one correspondence with the liquid outlet control valve body, each transmission motor is connected with a transmission block which can be inserted into a corresponding transmission groove, and when the closed door body cover is used for detecting the upper side of the detection shell, a closed detection cavity is formed between the inner wall of the detection shell and the lower side of the closed door body.
As a preferable aspect of the detection device of the present invention, wherein: the closed door body is also connected with a heating plate.
As a preferable aspect of the detection device of the present invention, wherein: and a peristaltic pump for driving liquid in the liquid storage tank to flow backwards is further arranged in the detection shell.
As a preferable aspect of the detection device of the present invention, wherein: the detection shell is internally connected with a lifting linear driver, the lifting linear driver is connected with a lifting rod which extends upwards and can reciprocate in the height direction, and the upper end of the lifting rod is connected to the lower side of the movable plate.
The utility model has the beneficial effects that: the utility model has simple structure and high detection efficiency, is convenient to carry, and realizes the detection of markers such as throat swabs and the like.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise. Wherein:
FIG. 1 is a perspective view of a microfluidic reaction assembly of the present invention above a detection housing.
FIG. 2 is a perspective view of the microfluidic reaction assembly of the present invention in a detection housing.
Fig. 3 is a three-dimensional structure view of the present invention with the detection case hidden.
Fig. 4 is an exploded view of the microfluidic reaction assembly according to the present invention.
FIG. 5 is an exploded view of a microfluidic reaction chip according to the present invention.
FIG. 6 is a first perspective view of a microfluidic reaction chip according to the present invention.
FIG. 7 is a second perspective view of the microfluidic reaction chip according to the present invention.
Fig. 8 is a perspective view of the first chip body according to the present invention.
In the figure, 100 microfluidic reaction components, 101 mounting seats, 101a mounting grooves, 102 microfluidic reaction chips, 102a first chip body, 102a1 liquid inlet components, 102a1-1 liquid storage tank, 102a1-2 liquid inlet holes, 102a1-3 liquid outlet channels, 102a1-4 liquid outlet valve sleeves, 102a1-5 liquid outlet reaction tank, 102a1-6 liquid outlet holes, 102a1-7 liquid outlet holes, 102b second chip body, 102b1 waste liquid holes, 102b2 reaction counter bores, 102c third chip body, 102c1 waste liquid tank, 103 glass plate, 104 control valve body, 104a control liquid hole, 104b transmission tank, 200 detector, 201 detection door body, 202 movable plate, peristaltic lifting rod, 204 detection shell, 205 heating plate, 206 transmission motor, 207 transmission block, 208 pump, 209 image collector, 210 lifting linear driver.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
Furthermore, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the utility model. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
Example 1
Referring to fig. 1 to 3, a first embodiment of the present invention provides a detection device, which can assist in performing detection of a marker in a microfluidic reaction chip 102.
A detection device comprises a microfluidic reaction assembly 100 and a detector 200, wherein the microfluidic reaction assembly 100 comprises a mounting seat 101, one upward end of the mounting seat 101 is provided with a mounting groove 101a, one downward end of the mounting seat 101 is provided with a first detection port communicated with the mounting groove 101a, a glass plate 103 is arranged in the first detection port, and the mounting seat 101 is provided with a microfluidic reaction chip 102 for detecting the concentration of a marker through the mounting groove 101 a; the detecting instrument 200 comprises a detecting shell 204, an image collector 209 is installed in the detecting shell 204, a movable plate 202 capable of moving in the height direction is connected in the detecting shell 204, a lifting linear driver 210 is connected in the detecting shell 204, a lifting rod 203 which extends upwards and can reciprocate in the height direction is connected on the lifting linear driver 210, the upper end of the lifting rod 203 is connected to the lower side of the movable plate 202, a mounting seat 101 is placed on the upper side of the movable plate 202, a second detecting opening corresponding to the first detecting opening is formed in the movable plate 202, and the image collector 209 is located right below the second detecting opening.
Further, a liquid outlet control valve body 104 for controlling liquid outlet is rotatably connected to the microfluidic reaction chip 102, the upper end of the liquid outlet control valve body 104 abuts against the upper side of the microfluidic reaction chip 102, and the upward end of the liquid outlet control valve body 104 is provided with a non-circular transmission groove 104 b.
Further, the upper end of the detection shell 204 is rotatably connected with a closed door body 201, one side of the closed door body 201, which is opposite to the detection shell 204, is connected with a plurality of transmission motors 206 which are in one-to-one correspondence with the liquid outlet control valve bodies 104, the transmission motors 206 are connected with transmission blocks 207 which can be inserted into the corresponding transmission grooves 104b, and when the closed door body 201 covers the upper side of the detection shell 204, a closed detection cavity is formed between the inner wall of the detection shell 204 and the lower side of the closed door body 201.
Furthermore, the sealing door 201 is also connected with a heating plate 205.
During nucleic acid detection, the heating plate 205 is controlled to work, heating is carried out according to amplification requirements to amplify RNA, and in the amplification process, a fluorophore signal generated in the amplification process is shot by the image collector 209 (preferably an astronomical grade freezing CCD camera).
Further, a peristaltic pump 208 for driving the liquid to flow backwards is further installed in the detection shell 204; when the liquid is fed, the reagent is driven to flow backwards by the peristaltic pump 208 according to the requirement of the liquid feeding.
Example 2
Referring to fig. 4 to 8, a second embodiment of the present invention is provided, which provides a detection apparatus capable of further performing a mixing reaction of a plurality of reagents.
A detection device, wherein a microfluidic reaction chip 102 comprises a first chip body 102a, a second chip body 102b and a third chip body 102c which are sequentially connected together from top to bottom, a plurality of groups of liquid inlet assemblies 102a1 are arranged on the first chip body 102a, each liquid inlet assembly 102a1 comprises a plurality of liquid storage tanks 102a1-1 arranged in the first chip body 102a and liquid outlet channels 102a1-3 connected with the liquid storage tanks 102a1-1, a plurality of liquid inlet holes 102a1-2 which correspond to and are communicated with the liquid storage tanks 102a1-1 one by one are arranged at the upward end of the first chip body 102a, a liquid outlet reaction tank 102a1-5 is arranged at the downward end of the first chip body 102a1-3, a liquid outlet valve sleeve 102a1-4 is connected on the first chip body 102a between the liquid outlet reaction tank 102a1-5, one end of the liquid outlet channel 102a1-3 far away from the liquid storage tank 102a1-1 can be communicated with the liquid outlet reaction tank 102a1-5 through the liquid outlet valve sleeve 102a1-4, liquid outlet holes 102a1-6 which are in one-to-one correspondence with the liquid outlet channels 102a1-3 are formed in the liquid outlet valve sleeve 102a1-4, a liquid outlet 102a1-7 which is communicated with the liquid outlet reaction groove 102a1-5 is formed in one end, far away from the liquid outlet channel 102a1-3, of the liquid outlet valve sleeve 102a1-4, a plurality of reaction counter bores 102b2 are arranged at one upward end of the second chip body 102b, the liquid outlet reaction groove 102a1-5 covers the corresponding reaction counter bore 102b2, a plurality of waste liquid holes 102b1 which are in one-to-one correspondence with the reaction counter bores are further formed in the second chip body 102b, the waste liquid holes 102b1 are arranged far away from the liquid outlet valve sleeve 102a1-4, and a waste liquid pool 102c1 which is communicated with the waste liquid holes 102b1 is arranged at one upward end of the third chip body 102 c.
Furthermore, the first chip body 102a is rotatably connected with a liquid outlet control valve body 104 through a liquid outlet valve sleeve 102a1-4, the liquid outlet control valve body 104 is provided with a control liquid hole 104a capable of communicating with the liquid outlet hole 102a1-6, when the control liquid hole 104a is communicated with the liquid outlet hole 102a1-6, the liquid in the corresponding liquid storage tank 102a1-1 sequentially passes through the liquid outlet channel 102a1-3, the liquid outlet holes 102a1-6, the control liquid hole 104a and the liquid outlet control valve body 104 and then enters the area where the liquid outlet reaction tank 102a1-5 is located, a plurality of liquid outlet holes 102a1-6 which are in one-to-one correspondence and communication with the liquid outlet channel 102a1-3 are distributed on the liquid outlet valve sleeve 102a1-4, and one end of the liquid outlet valve sleeve 102a1-4, which is far away from the liquid outlet channel 102a1-3, is provided with a liquid outlet 102a1-7 which is arranged towards the liquid outlet reaction tank 102a 1-5.
The peristaltic pump 208 drives the reagents in the liquid storage tanks 102a1-1 to flow towards the liquid outlet reaction tank 102a1-5 through the corresponding liquid inlet holes 102a1-2, so that the reagents in different liquid storage tanks 102a1-1 enter the area where the liquid outlet reaction tank 102a1-5 is located according to the sample introduction sequence for reaction, and the redundant solution flows into the waste liquid tank 102c1 through the waste liquid hole 102b 1; during detection, the closed door body 201 is closed, the transmission block 207 is inserted into the transmission groove 104b, the transmission motor 206 acts to enable the control liquid hole 104a to be aligned with the liquid storage tank 102a1-1 needing liquid outlet, reagents in the liquid storage tank 102a1-1 sequentially pass through the corresponding liquid outlet channel 102a1-3, the liquid outlet hole 102a1-6, the control liquid hole 104a and the liquid outlet control valve body 104 and then enter the area where the liquid outlet reaction groove 102a1-5 is located, the reagents react in the reaction counter bore 102b2, the image collector 209 shoots fluorophore signals generated in the reaction process, and waste liquid enters the waste liquid tank 102c1 of the third chip body 102c through the waste liquid hole 102b1 to achieve waste liquid recovery.
It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.
Claims (10)
1. A detection device, characterized by: which comprises the steps of preparing a mixture of a plurality of raw materials,
the microfluidic reaction assembly (100) comprises a mounting seat (101), wherein an installation groove (101a) is formed in the upward end of the mounting seat (101), a first detection port communicated with the installation groove (101a) is formed in the downward end of the mounting seat (101), and a microfluidic reaction chip (102) for detecting the concentration of a marker is installed on the mounting seat (101) through the installation groove (101 a);
detector (200), detector (200) are including detecting casing (204), install image collector (209) in detecting casing (204), it has fly leaf (202) that can remove on the direction of height to detect casing (204) in-connection, mount pad (101) are placed at fly leaf (202) upside, it has the second detection mouth that corresponds with first detection mouth to open on fly leaf (202), image collector (209) are under the second detection mouth.
2. The detection device of claim 1, wherein: the microfluidic reaction chip (102) comprises a first chip body (102a), a second chip body (102b) and a third chip body (102c) which are sequentially connected together from top to bottom, wherein a plurality of groups of liquid inlet assemblies (102a1) are arranged on the first chip body (102a), each liquid inlet assembly (102a1) comprises a plurality of liquid storage pools (102a1-1) arranged in the first chip body (102a) and liquid outlet channels (102a1-3) connected with the liquid storage pools (102a1-1), a plurality of liquid inlet holes (102a1-2) which are in one-to-one correspondence with and communicated with the liquid storage pools (102a1-1) are arranged at the upward end of the first chip body (102a), a liquid outlet reaction groove (102a1-5) is arranged at the downward end of the first chip body (102a), and a liquid outlet valve is connected to the first chip body (102a) between the liquid outlet channel (102a1-3) and the liquid outlet reaction groove (102a1-5) The liquid outlet valve comprises a sleeve (102a1-4), one end, far away from a liquid storage tank (102a1-1), of the liquid outlet channel (102a1-3) can be communicated with a liquid outlet reaction tank (102a1-5) through a liquid outlet valve sleeve (102a1-4), a plurality of reaction counter bores (102b2) are arranged at the upward end of the second chip body (102b), the liquid outlet reaction tank (102a1-5) covers the corresponding reaction counter bores (102b2), a plurality of waste liquid holes (102b1) corresponding to the reaction counter bores one to one are further formed in the second chip body (102b), the waste liquid holes (102b1) are far away from the liquid outlet valve sleeve (102a1-4), and a waste liquid tank (102c1) communicated with the waste liquid holes (102b1) is arranged at the upward end of the third chip body (102 c).
3. The detection device of claim 2, wherein: a plurality of liquid outlet holes (102a1-6) which are in one-to-one correspondence and are communicated with the liquid outlet channels (102a1-3) are distributed on the liquid outlet valve sleeve (102a1-4), and a liquid outlet (102a1-7) which is arranged towards the liquid outlet reaction tank (102a1-5) is arranged at one end of the liquid outlet valve sleeve (102a1-4) far away from the liquid outlet channels (102a 1-3).
4. A testing device according to claim 3, wherein: the first chip body (102a) is rotatably connected with a liquid outlet control valve body (104) through a liquid outlet valve sleeve (102a1-4), the liquid outlet control valve body (104) is provided with a control liquid hole (104a) capable of being communicated with the liquid outlet hole (102a1-6), and when the control liquid hole (104a) is communicated with the liquid outlet hole (102a1-6), liquid in the corresponding liquid storage pool (102a1-1) sequentially passes through a liquid outlet channel (102a1-3), the liquid outlet hole (102a1-6), the control liquid hole (104a) and the liquid outlet control valve body (104) and then enters the area where the liquid outlet reaction tank (102a1-5) is located.
5. The detection device of claim 4, wherein: the upper end of the liquid outlet control valve body (104) is abutted against the upper side of the first chip body (102 a).
6. The detection apparatus according to claim 3 or 4, wherein: and a non-circular transmission groove (104b) is formed in the upward end of the liquid outlet control valve body (104).
7. The detection device of claim 6, wherein: the upper end that detects casing (204) rotationally is connected with the closure door body (201), one side that the closure door body (201) detected casing (204) relatively is connected with a plurality of and goes out liquid control valve body (104) one-to-one transmission motor (206), be connected with on transmission motor (206) and inject transmission piece (207) in corresponding transmission groove (104b), the closure door body (201) lid is when detecting casing (204) upside, detects and forms the confined detection chamber between casing (204) inner wall and the closure door body (201) downside.
8. The detection device of claim 7, wherein: the closed door body (201) is also connected with a heating plate (205).
9. The detecting device according to any one of claims 1 to 5, wherein: a peristaltic pump (208) for driving liquid in the liquid storage tank (102a1-1) to flow backwards is also installed in the detection shell (204).
10. The detecting device according to any one of claims 1 to 5, wherein: detect casing (204) in-connection have lift linear actuator (210), be connected with on lift linear actuator (210) and upwards stretch out and can be reciprocating motion's lifter (203) in the direction of height, the upper end of lifter (203) is connected at fly leaf (202) downside.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122528002.6U CN216093736U (en) | 2021-10-20 | 2021-10-20 | Detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122528002.6U CN216093736U (en) | 2021-10-20 | 2021-10-20 | Detection device |
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| CN216093736U true CN216093736U (en) | 2022-03-22 |
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| CN202122528002.6U Active CN216093736U (en) | 2021-10-20 | 2021-10-20 | Detection device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115508559A (en) * | 2022-08-26 | 2022-12-23 | 扬州大学 | Drug detection device |
| CN116990288A (en) * | 2023-07-24 | 2023-11-03 | 扬州大学 | Micro-fluidic-based cadmium ion logic detection device |
-
2021
- 2021-10-20 CN CN202122528002.6U patent/CN216093736U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115508559A (en) * | 2022-08-26 | 2022-12-23 | 扬州大学 | Drug detection device |
| CN116990288A (en) * | 2023-07-24 | 2023-11-03 | 扬州大学 | Micro-fluidic-based cadmium ion logic detection device |
| CN116990288B (en) * | 2023-07-24 | 2024-02-06 | 扬州大学 | Micro-fluidic-based cadmium ion logic detection device |
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