CN110239804A - Lancing device and microfluidic device - Google Patents
Lancing device and microfluidic device Download PDFInfo
- Publication number
- CN110239804A CN110239804A CN201910649387.6A CN201910649387A CN110239804A CN 110239804 A CN110239804 A CN 110239804A CN 201910649387 A CN201910649387 A CN 201910649387A CN 110239804 A CN110239804 A CN 110239804A
- Authority
- CN
- China
- Prior art keywords
- component
- compression bar
- link block
- lancing device
- driving
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000012530 fluid Substances 0.000 claims abstract description 43
- 238000006243 chemical reaction Methods 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 230000006835 compression Effects 0.000 claims description 62
- 238000007906 compression Methods 0.000 claims description 62
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 13
- 230000007246 mechanism Effects 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 4
- 238000001514 detection method Methods 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000007259 addition reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65B—MACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
- B65B69/00—Unpacking of articles or materials, not otherwise provided for
- B65B69/0033—Unpacking of articles or materials, not otherwise provided for by cutting
- B65B69/0041—Unpacking of articles or materials, not otherwise provided for by cutting by puncturing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
Abstract
This application involves field of biological detection, in particular to a kind of lancing device and microfluidic device.Lancing device is used to seal in chemical reaction, including punctures component, seal assembly and driving assembly;The driving component is connect with the component that punctures, and the driving component can drive the working end for puncturing component to move to close to or far from fluid packet;The seal assembly be located at it is described puncture between component and fluid packet, the seal assembly is used to puncture the fluid packet and when far from the fluid packet in the component that punctures, and the liquid flowed out in component and the fluid packet that punctures is kept being isolated.All very high in assembling product and the upper difficulty of design in a manner of solving valve and pipeline existing in the prior art, the price so as to cause product is higher, is unfavorable for the problem of promoting and applying.
Description
Technical field
This application involves field of biological detection, in particular to a kind of lancing device and microfluidic device.
Background technique
Chemical reaction for closed system, reaction environment are isolated from the outside world, and addition reaction raw materials can only be in reactor
The reaction raw materials of needs are spatially segregated from storage by portion, are then discharged when needed to form contact and mixing, are occurred anti-
It answers.
The key request of above-mentioned mode is can safety and stability storage plurality of raw materials and fast and easy elimination
Space isolation.The mode generallyd use is all valve and circuit design, to realize the addition of the reaction raw materials in sealing precursor reactant,
But the mode of valve and pipeline is all very high in assembling product and the upper difficulty of design, the price so as to cause product is higher, no
Conducive to popularization and application.
Summary of the invention
The embodiment of the present application is designed to provide a kind of lancing device, and the structure of the lancing device is simple, convenient for assembling
And it is easy to operate, be conducive to promote and apply.
In a first aspect, the embodiment of the present invention provides a kind of lancing device, it is used to seal in chemical reaction, including puncture group
Part, seal assembly and driving assembly;
The driving component is connect with the component that punctures, and the driving component can drive the work for puncturing component
It holds to close to or far from fluid packet and moves;
The seal assembly punctures between component and fluid packet described in being located at, and the seal assembly is used to puncture group described
The fluid packet is punctured and when far from the fluid packet by part, and the liquid flowed out in component and the fluid packet that punctures is protected
Hold isolation.
In alternative embodiments, the component that punctures further includes more compression bar arms;
The every compression bar arm is equipped at least one described working end, and the side of each compression bar arm is equipped at least
One connector for abutting with the driving component, the other side are equipped with reset spring, and the driving component can squeeze institute
Connector is stated, and compresses the reset spring, so that the compression bar arm pumps.
In alternative embodiments, the driving component includes driving mechanism and sliding block;
The sliding block is located at one end of the compression bar arm, and the sliding block is equipped with recessed portion, when the connector with it is described
When recessed portion cooperates, the reset spring resets.
In alternative embodiments, the driving mechanism includes driving motor, driving member, screw rod and linkage seat;
One end of the driving member is connect with the output end of the driving motor, the other end of the driving member and the silk
Bar connection, the linkage seat is mounted on the screw rod, and the linkage seat can back and forth be transported along the extending direction of the screw rod
It is dynamic;
The linkage seat is connected with the slide block.
In alternative embodiments, the driving mechanism further includes support base, and the support base is located at close to the biography
One end of moving part, the screw rod pass through support base and connect with the linkage seat.
In alternative embodiments, the component that punctures further includes shaft;
The compression bar arm is three, three compression bar arm parallel intervals settings, one end of three compression bar arms with
The shaft rotation connection, the other end of three compression bar arms are equipped at least one described working end, and two neighboring institute
State the line of the connector of compression bar arm upper end not on the same line.
In alternative embodiments, the lancing device further includes pedestal, set on the pedestal there are three be used for and institute
The locating slot of compression bar arm cooperation is stated, the shaft passes through three locating slots and fixes three compression bar arms, the positioning
At least one guide rail being connected with the slide block, the extension side of the extending direction of the guide rail and the locating slot are equipped with above slot
To identical.
In alternative embodiments, the sliding block includes that main body, the first link block, the second link block are connected with third
Block;
First link block, the second link block connect block gap with third and are located at the main body close to the compression bar arm
One end, one end of first link block, the second link block and the third link block are located at the end of the main body, and described
The other end of one link block, the second link block and the third link block is in inclined-plane setting, along prolonging for first link block
It stretches direction and sets that there are two the first grooves with the connector cooperation on the wherein first compression bar arm, on second link block
The second groove equipped with one with the connector cooperation on the wherein second compression bar arm.
In alternative embodiments, the pedestal is equipped with reseting sensor, and the reseting sensor is located at the base
The side of seat, the reseting sensor is for resetting the linkage seat.
In alternative embodiments, the seal assembly is pellosil.
It is another object of the present invention to provide a kind of microfluidic devices, have lancing device as described above.
Through the above technical solutions, driving assembly can drive movement of the working end for puncturing component to fluid packet, and will
Fluid wrapping is broken so that the liquid in fluid packet flows out, and is chemically reacted, is not needed by way of pipeline and valve in this way
It is chemically reacted, in addition seal assembly is flowing the liquid in fluid packet between the working end for puncturing component and fluid packet
It is to ensure the accuracy of reaction in closed environment out and during reaction.
Detailed description of the invention
Technical solution in ord to more clearly illustrate embodiments of the present application will make below to required in the embodiment of the present application
Attached drawing is briefly described, it should be understood that the following drawings illustrates only some embodiments of the application, therefore should not be seen
Work is the restriction to range, for those of ordinary skill in the art, without creative efforts, can be with
Other relevant attached drawings are obtained according to these attached drawings.
Fig. 1 is the perspective view of lancing device provided by the embodiments of the present application;
Fig. 2 is the cross-sectional view of lancing device provided by the embodiments of the present application;
Fig. 3 is lancing device sliding block provided by the embodiments of the present application and the structural schematic diagram for puncturing component mating connection;
Fig. 4 is the structural schematic diagram of the sliding block of lancing device provided by the embodiments of the present application;
Fig. 5 is the cross-sectional view punctured when component operation terminal spine breaks fluid packet in lancing device provided by the embodiments of the present application.
Icon: 100- punctures component;110- compression bar arm;The working end 120-;130- connector;140- reset spring;150-
Shaft;200- driving assembly;210- driving motor;220- driving member;230- screw rod;240- linkage seat;250- sliding block;251- master
Body;The first link block of 252-;The second link block of 253-;254- third link block;260- support base;300- seal assembly;400-
Pedestal;410- guide rail;420- reseting sensor;500- fluid packet.
Specific embodiment
To keep the purposes, technical schemes and advantages of the embodiment of the present application clearer, below in conjunction with the embodiment of the present application
In attached drawing, the technical scheme in the embodiment of the application is clearly and completely described.Obviously, described embodiment is
Some embodiments of the present application, instead of all the embodiments.The application being usually described and illustrated herein in the accompanying drawings is implemented
The component of example can be arranged and be designed with a variety of different configurations.
In the description of the present application, it should be noted that the orientation or positional relationship of the instructions such as term "inner", "outside" is base
In orientation or positional relationship shown in the drawings or this application product using when the orientation or positional relationship usually put, only
It is the application and simplified description for ease of description, rather than the device or element of indication or suggestion meaning must have specifically
Orientation is constructed and operated in a specific orientation, therefore should not be understood as the limitation to the application.In addition, term " first ", " the
Two " etc. are only used for distinguishing description, are not understood to indicate or imply relative importance.
In the description of the present application, it is also necessary to which explanation is unless specifically defined or limited otherwise, term " setting ",
" connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected;It can be
It is connected directly, the connection inside two elements can also be can be indirectly connected through an intermediary.For the common of this field
For technical staff, the concrete meaning of above-mentioned term in this application can be understood with concrete condition.
As shown in figs. 1-3 and 5, a kind of lancing device for sealing in chemical reaction, including puncture component 100, sealing
Component 300 and driving assembly 200;The driving component 200 is connect with the component 100 that punctures, and the driving component 200 can
The working end 120 that component 100 is punctured described in driving is moved to close to or far from fluid packet 500;The seal assembly 300 is located at institute
It states and punctures between component 100 and fluid packet 500, the seal assembly 300 is used for the fluid packet in the component 100 that punctures
It punctures and when far from the fluid packet 500, the liquid flowed out in component 100 and the fluid packet 500 that punctures keeps being isolated.
Wherein, the seal assembly 300 is preferably pellosil.Pellosil is being punctured 100 working end 120 of component to leaning on
When the power of nearly 500 1 end motion of fluid packet, due to the characteristic of pellosil itself, cause to puncture fluid packet 500 puncturing component 100
When, only deformation occurs for pellosil itself, and the working end 120 that can't be punctured component 100 punctures, and then ensures fluid packet
When liquid in 500 flows out, reaction compartment remains confined space.
Since liquid packet bottom micro-channels film is mantle, thus needle of breaking seal can continue up to reach puncture liquid packet
Sealed bottom film is without speckling with the purpose of solution in liquid pack assembly chamber.
In this implementation, driving assembly 200 can drive movement of the working end 120 for puncturing component 100 to fluid packet 500,
And fluid packet 500 is punctured so that the liquid in fluid packet 500 flows out, chemically reacted, do not need in this way through pipeline and
The mode of valve is chemically reacted, in addition seal assembly 300 the working end 120 that punctures component 100 and fluid packet 500 it
Between, making the liquid in fluid packet 500 is to ensure the accurate of reaction in closed environment during outflow and reaction
Property.
In alternative embodiments, the component 100 that punctures further includes more compression bar arms 110;The every compression bar arm
110 are equipped at least one described working end 120, and the side of each compression bar arm 110 is used for equipped at least one and institute
The connector 130 of the abutting of driving assembly 200 is stated, the other side is equipped with reset spring 140, and the driving component 200 can squeeze institute
Connector 130 is stated, and compresses the reset spring 140, so that the compression bar arm 110 pumps.
Wherein, one end that connector 130 is abutted with driving assembly 200 is bearing or idler wheel, with connector 130 and driving group
Frictional force between part 200.
In addition, working end 120 is unpacking needle.
In the present embodiment, driving assembly 200 in the process of moving, can abut, and produce with the outer profile of connector 130
The raw extruding force to compression bar arm 110, due to the setting of reset spring 140, in this way with the movement of driving assembly 200, Jin Erke
So that mobile formation corresponding motion profile of the compression bar arm 110 with driving assembly 200, to drive the working end of compression bar arm 110
Position where 120 to fluid packet 500 moves, and can restore, to realize the function of puncturing fluid packet 500.
In alternative embodiments, the driving component 200 includes driving mechanism and sliding block 250;The sliding block 250
In one end of the compression bar arm 110, the sliding block 250 is equipped with recessed portion, when the connector 130 cooperates with the recessed portion
When, the reset spring 140 resets.
Wherein, the quantity of recessed portion can be one, two or three etc., be adjusted according to specific requirement.
In the present embodiment, driving mechanism drives sliding block 250 to slide, and the glide direction of sliding block 250 and compression bar arm 110
Extending direction is identical, and in this way during sliding block 250 slides, connector 130 will receive the extruding force of sliding block 250, to make
The working end 120 of compression bar arm 110 can be moved to the position where fluid packet 500, because 110 lower end of compression bar arm is equipped with reset spring
140, when connector 130 is by recessed portion, reset spring 140 resets, and working end 120 is far from fluid packet 500 at this time.
In alternative embodiments, the driving mechanism includes driving motor 210, driving member 220, screw rod 230 and connection
Dynamic seat 240;One end of the driving member 220 is connect with the output end of the driving motor 210, the driving member 220 it is another
End is connect with the screw rod 230, and the linkage seat 240 is mounted on the screw rod 230, and the linkage seat 240 can be along institute
The extending direction for stating screw rod 230 moves back and forth;The linkage seat 240 is connect with the sliding block 250.
Wherein, driving member 220 is preferably gear drive.
In the present embodiment, driving motor 210 drives screw rod 230 to rotate by driving member 220, and screw rod 230 and linkage seat
240 connections, when screw rod 230 rotates, linkage seat 240 is connect with sliding block 250, mobile so as to band movable slider 250, to realize
Fluid packet 500 is punctured and is playbacked by the working end 120 being connected on compression bar arm 110.
In alternative embodiments, the driving mechanism further includes support base 260, and the support base 260 is located at close
One end of the driving member 220, the screw rod 230 pass through support base 260 and connect with the linkage seat 240.
In the present embodiment, the setting of support base 260 can be improved convenience when screw rod 230 is installed, and screw rod 230
Stability during rotation can also be protected.
In alternative embodiments, the component 100 that punctures further includes shaft 150;The compression bar arm 110 is three,
Three 110 parallel interval of compression bar arm settings, one end of three compression bar arms 110 are rotatablely connected with the shaft 150,
The other end of three compression bar arms 110 is equipped at least one described working end 120, and the two neighboring compression bar arm 110
The line of the connector 130 of upper end is not on the same line.
As shown in figure 4, further, the sliding block 250 includes main body 251, the first link block 252, the second link block 253
With third link block 254;First link block 252, the second link block 253 and the interval of third link block 254 are located at the master
Body 251 is close to one end of the compression bar arm 110, first link block 252, the second link block 253 and the third link block
254 one end is located at the end of the main body 251, and first link block 252, the second link block 253 are connected with the third
The other end of block 254 be in inclined-plane setting, along the extending direction of first link block 252 set there are two with wherein first institute
The first groove that the connector 130 on compression bar arm 110 cooperates is stated, second link block 253 is equipped with one and wherein second
The second groove that connector 130 on a compression bar arm 110 cooperates.
In the present embodiment, compression bar arm 110 is preferably three, and one end of three compression bar arms 110 is connected by shaft 150,
It is equipped at least one working end 120 on three compression bar arms 110, is equipped in one end that sliding block 250 can be contacted with compression bar arm 110
First link block 252, the second link block 253 and third link block 254, during main body 251 is mobile, the first link block
252, one end of the second link block 253 and third link block 254 is arranged in inclined-plane, the connector being located on compression bar arm 110 in this way
130 can preferably slide on the first link block 252, the second link block 253 and third link block 254, and two of them
At least two connectors 130 and the first link block 252 and the second link block 253 on compression bar arm 110 be equipped with the first groove and
The cooperation of second groove, so as to realize in the unidirectional sliding process of sliding block 250, the working end 120 of two of them compression bar arm 110 can
It realizes flexible.
In alternative embodiments, the lancing device further includes pedestal 400, set on the pedestal 400 there are three use
In the locating slot cooperated with the compression bar arm 110, the shaft 150 passes through three locating slots for three compression bar arms
110 is fixed, at least one guide rail 410 connecting with the sliding block 250 is equipped with above the locating slot, and the guide rail 410 prolongs
It is identical as the extending direction of the locating slot to stretch direction.
Wherein, pedestal 400 is equipped at least one pilot hole, and pilot hole and the working end 120 for puncturing component 100 are thought pair
It answers, working end 120 can stretch along pilot hole, so that fluid packet 500 punctures.
In the present embodiment, locating slot is equipped on pedestal 400, locating slot can be such that three compression bar arms 110 rotationally set
It sets, makes whole stability more preferably, in addition the setting of guide rail 410 can make sliding block 250 sliding along the extending direction of guide rail 410
It is dynamic, it ensures that it is abutted with the connector 130 on compression bar arm 110 in the process of movement, drives the work being located on compression bar arm 110
End 120 punctures fluid packet 500.
In alternative embodiments, the pedestal 400 is equipped with reseting sensor 420, and the reseting sensor 420 is set
In the side of the pedestal 400, the reseting sensor 420 is for resetting the linkage seat 240.
In the present embodiment, the setting of reseting sensor 420 can make single unit system once puncture the dynamic of fluid packet 500 doing
The initial position that can restore after work, the movement to ensure each task are identical.
It is another object of the present invention to provide a kind of microfluidic devices, have lancing device as described above.It is produced
Raw beneficial effect is identical as the effect of lancing device, repeats no more.
It should be noted that in the absence of conflict, the feature in embodiment in the application can be combined with each other.
The foregoing is merely preferred embodiment of the present application, are not intended to limit this application, for the skill of this field
For art personnel, various changes and changes are possible in this application.Within the spirit and principles of this application, made any to repair
Change, equivalent replacement, improvement etc., should be included within the scope of protection of this application.
Claims (11)
1. a kind of lancing device, for sealing in chemical reaction, which is characterized in that including puncturing component, seal assembly and driving
Component;
The driving component is connect with the component that punctures, the driving component can drive the working end for puncturing component to
It is moved close to or far from fluid packet;
The seal assembly punctures between component and fluid packet described in being located at, and the seal assembly is used for will in the component that punctures
The fluid packet punctures and when far from the fluid packet, by it is described puncture the liquid flowed out in component and the fluid packet keep every
From.
2. lancing device according to claim 1, which is characterized in that the component that punctures further includes more compression bar arms;
The every compression bar arm is equipped at least one described working end, and the side of each compression bar arm is equipped at least one
Connector for abutting with the driving component, the other side are equipped with reset spring, and connector the driving component can squeeze
The connector, and the reset spring is compressed, so that the compression bar arm pumps.
3. lancing device according to claim 2, which is characterized in that the driving component includes driving mechanism and sliding block;
The sliding block is located at one end of the compression bar arm, and the sliding block is equipped with connector recessed portion, when the connector and institute
When stating recessed portion cooperation, the reset spring resets.
4. lancing device according to claim 3, which is characterized in that the driving mechanism include driving motor, driving member,
Screw rod and linkage seat;
One end of the driving member is connect with the output end of the driving motor, and the other end of the driving member and the screw rod connect
It connects, the linkage seat is mounted on the screw rod, and the linkage seat can be moved back and forth along the extending direction of the screw rod;
The linkage seat is connected with the slide block.
5. lancing device according to claim 4, which is characterized in that the driving mechanism further includes support base, the branch
Support seat is located at close to one end of the driving member, and the screw rod passes through support base and connect with the linkage seat.
6. lancing device according to claim 4, which is characterized in that the component that punctures further includes shaft;
The compression bar arm is three, three compression bar arm parallel intervals settings, one end of three compression bar arms with it is described
Shaft rotation connection, the other end of three compression bar arms are equipped at least one described working end, and the two neighboring pressure
The line of the connector of lever arm upper end is not on the same line.
7. lancing device according to claim 6, which is characterized in that the lancing device further includes pedestal, the pedestal
On set there are three for the locating slot with three compression bar arms cooperations, the shaft passes through three locating slots for three institutes
It states compression bar arm to fix, at least one guide rail being connected with the slide block, the extension side of the guide rail is equipped with above the locating slot
To identical as the extending direction of the locating slot.
8. lancing device according to claim 7, which is characterized in that the sliding block includes main body, the first link block, second
Link block and third link block;
First link block, the second link block connect block gap with third and are located at the main body close to the one of the compression bar arm
End, one end of first link block, the second link block and the third link block are located at the end of the main body, and described first
The other end of link block, the second link block and the third link block is in inclined-plane setting, along the extension of first link block
Direction is set there are two the first groove with the connector cooperation on the wherein first compression bar arm, is set on second link block
There are the second groove of one with the connector cooperation on the wherein second compression bar arm.
9. lancing device according to claim 7, which is characterized in that the pedestal is equipped with reseting sensor, described multiple
Level sensor is located at the side of the pedestal, and the reseting sensor is for resetting the linkage seat.
10. lancing device according to claim 1, which is characterized in that the seal assembly is pellosil, the working end
For needle of breaking seal.
11. a kind of microfluidic device, which is characterized in that have such as the described in any item lancing devices of claim 1-10.
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CN201910649387.6A CN110239804A (en) | 2019-07-17 | 2019-07-17 | Lancing device and microfluidic device |
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CN201910649387.6A CN110239804A (en) | 2019-07-17 | 2019-07-17 | Lancing device and microfluidic device |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111187713A (en) * | 2020-02-21 | 2020-05-22 | 厦门大学 | Micro-fluidic chip's puncture device and micro-fluidic chip detecting system |
CN111187714A (en) * | 2020-02-21 | 2020-05-22 | 厦门大学 | Micro-fluidic chip assembly and micro-fluidic chip detection system |
CN114100715A (en) * | 2021-11-22 | 2022-03-01 | 江苏液滴逻辑生物技术有限公司 | Sample injection mode for digital micro-fluidic chip |
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