CN114099388B - Anti-wrinkle composition and preparation method and application thereof - Google Patents
Anti-wrinkle composition and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses an anti-wrinkle composition, a preparation method and application thereof, and relates to the technical field of cosmetics. The anti-wrinkle composition takes the hydrolyzed okra coffee extract and the benstonia tobacco extract as raw materials, and the content and the mass ratio of the hydrolyzed okra coffee extract and the benstonia tobacco extract are controlled, so that the obtained anti-wrinkle composition can improve the water content of skin, promote the generation of type I collagen, up-regulate genes related to the generation of epidermal lipid, improve the integrity and the barrier function of the skin, increase the thickness of the epidermis, fill epidermal lipid and promote the generation of collagen and hyaluronic acid, and the two active ingredients have the synergistic effect mutually, so that the anti-wrinkle composition has the effects of more comprehensively and remarkably reducing the depth and the area of wrinkles, weakening the wrinkles, tightening pores and improving the skin glossiness.
Description
Technical Field
The invention relates to the technical field of cosmetics, and particularly relates to an anti-wrinkle composition, and a preparation method and application thereof.
Background
With aging, skin aging is an inevitable aging phenomenon. Skin aging can be divided into endogenous aging and exogenous aging, wherein the former mainly refers to aging caused by irresistible factors existing in the body, such as heredity, gravity, endocrine, immune function and the like, along with the increase of age; the latter aggravates the natural aging process mainly due to the effects of the environment, sunlight, health, living habits, and the like. Particularly, in the age with great living pressure, people often stay up, smoke, drink, use electronic products for a long time, use excessive eyes and the like to accelerate skin aging, and when the water content of the skin cuticle is about 20 percent and is sufficient, the skin is soft, smooth, fine and elastic. When the stratum corneum contains less than 10% of water due to lack of lipids, water or moisturizing factors, the normal structure of the stratum corneum is unstable, and the barrier function of the skin is affected, so that increased percutaneous water loss is caused, and the skin becomes dry, rough, dull, less elastic, more wrinkled, itchy, desquamated, and the like, and also, skin aging is accelerated. Among them, facial skin aging is particularly remarkable, the number of fibroblasts in the human body is gradually reduced, collagen and elastin secreted therefrom are decomposed and broken, and the moisturizing component hyaluronic acid is reduced, the stratum corneum is thinned, etc., thereby making the skin lose elasticity and forming wrinkles, which are a sign of skin aging.
Aging is an inevitable but postponable biological process, and with the improvement of living standard and the pursuit of beauty, people have more and more demands for anti-wrinkle cosmetics.
For example, Chinese patent application 201811429898.9 discloses an anti-wrinkle moisturizing skin-brightening composition comprising alkali-soluble ganoderan ferulic ester. The research shows that the alkali-soluble ganoderma lucidum polysaccharide ferulate has the anti-aging effect, and the anti-wrinkle moisturizing and skin-brightening composition has the anti-aging and anti-wrinkle effects by adding the alkali-soluble ganoderma lucidum polysaccharide ferulate, but the anti-wrinkle effect is not obvious.
In recent years, retinol is widely used as an effective anti-aging component in medicine and cosmetics, is widely applied to anti-aging and anti-wrinkle products, has obvious effect, and is favored by consumers. For example, the chinese patent application 202110728500.7 discloses a multi-dimensional anti-wrinkle composition, a cosmetic composition and a preparation method thereof, which relate to the technical field of cosmetics, and the multi-dimensional anti-wrinkle composition is composed of the following raw materials in parts by weight: retinol palmitate: 0.05-0.2 part; acetyl tetrapeptide-2: 0.02-0.2 part; acetyl hexapeptide-8: 0.02-0.2 part; split yeast fermentation lysate: 0.5-5 parts; auxiliary components: and (4) the balance. However, the particular physicochemical properties of retinol limit its use: has great sensitivity to light, heat, polar media and the like, is easy to decay into non-bioactive substances under common conditions, and brings difficulty to use and storage. And retinol is converted by enzymes on the skin surface to release retinoic acid, which has certain irritation.
Therefore, there is a need for the development of an anti-wrinkle composition having good water retention, pore tightening, skin gloss enhancement, low irritation and photosensitivity, and good stability.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide an anti-wrinkle composition, a preparation method and an application thereof.
The invention is realized by the following technical scheme.
In one aspect, the invention provides an anti-wrinkle composition, which comprises the following components in parts by weight: 1-20 parts of Nicotiana benthamiana extract; 0.5-15 parts of hydrolyzed coffee abelmoschus manihot extract.
Preferably, the composition comprises the following components in parts by weight: 5-15 parts of Nicotiana benthamiana extract; 1-12 parts of hydrolyzed coffee abelmoschus manihot extract.
Still preferably, the composition comprises the following components in parts by weight: 10-15 parts of Nicotiana benthamiana extract; 5-10 parts of hydrolyzed coffee abelmoschus manihot extract.
Further preferably, the mass ratio of the nicotiana benthamiana extract to the hydrolyzed okra extract is 1-5: 1.
In another aspect, the present invention provides a method for preparing the anti-wrinkle composition, comprising the steps of:
weighing the benstonia tobacco extract and the hydrolyzed coffee abelmoschus esculentus extract according to the formula dosage, and uniformly stirring to obtain the anti-wrinkle composition.
The trade name of the Nicotiana benthamiana extract is as follows: a Reesed; the purchase manufacturers are as follows: lipoture.
The trade name of the hydrolyzed coffee abelmoschus esculentus extract is as follows: phyto hydralon; the purchase manufacturers are as follows: ICHIMARU PHARCOS.
The Nicotiana benthamiana extract has activity similar to that of vitamin A, can promote the synthesis of type I collagen, can up-regulate genes related to epidermal lipid generation, improve the skin integrity and barrier function, increase the epidermal thickness and fill epidermal lipid, smooth wrinkles, has lower phototoxicity than vitamin A and vitamin A acid, has good chemical stability, is not influenced by light, can be used in the daytime, and is an effective anti-wrinkle active substance; the hydrolyzed coffee abelmoschus manihot extract has the effects of moisturizing, promoting the generation of collagen and hyaluronic acid and resisting wrinkles. The two active ingredients have mutual synergistic effect, and have the effects of reducing the depth and the area of wrinkles, fading the wrinkles, tightening pores and improving the skin glossiness more comprehensively and obviously.
In the implementation process, the invention unexpectedly discovers that the effect of firming pores, improving skin glossiness and reducing wrinkle depth can be better achieved by mixing the nicotiana benthamiana extract and the hydrolyzed coffee abelmoschus manihot extract.
In another aspect, the invention also provides the application of the composition in a skin care product, wherein the skin care product is a water agent, an emulsion, a cream, essence, a facial mask or gel.
A skin care product comprising an anti-wrinkle composition and an adjuvant; the auxiliary agent is selected from one or more of chelating agent, thickening agent, humectant, solubilizer, emulsifier, pH regulator, antiseptic, solvent, oily matrix and essence.
Wherein, the chelating agent is EDTA-2 Na.
The thickening agent is selected from one or more of acrylic acid (acrylate)/C10-30 alkanol acrylate cross-linked polymer, xanthan gum, carboxymethyl cellulose and hydroxyethyl cellulose.
Preferably, the thickener is selected from one or more of acrylic acid (ester)/C10-30 alkanol acrylate crosspolymer, xanthan gum and carboxymethyl cellulose.
In some preferred embodiments the thickener is an acrylic acid/C10-30 alkanol acrylate crosspolymer;
preferably, the mass ratio of the acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer to the anti-wrinkle composition is 1.5-6.5:30-65, and further preferably, the mass ratio of the acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer to the anti-wrinkle composition is 2:30, 1.5:40 or 0.1: 10.
In the implementation process of the invention, the fact that the release of effective components in the anti-wrinkle composition can be ensured under proper viscosity of the obtained skin care product by controlling the mass ratio of the acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer in the thickening agent to the anti-wrinkle composition is unexpectedly found, and the anti-wrinkle performance of the skin care product is improved.
The humectant is selected from one or more of glycerol, butanediol, 1, 3-butanediol, propylene glycol, dipropylene glycol, hydroxyethyl urea, sorbitol, hexylene glycol, dextran, allantoin and methyl propylene glycol.
Preferably, the humectant is selected from one or more of glycerol, 1, 3-butylene glycol, propylene glycol, hexylene glycol, allantoin and methyl propylene glycol.
The solubilizer is selected from one or more of oleyl polyether-3, oleyl polyether-5, oleyl polyether-20, PEG-40 hydrogenated castor oil and C12-13 alkanol polyether-15.
Preferably, the solubilizer is PEG-40 hydrogenated castor oil.
The emulsifier is one or more selected from span 60, tween 60, glyceryl monostearate and self-emulsifying glyceryl monostearate.
Preferably, the emulsifier is selected from one or more of tween 60, span 60 and glyceryl monostearate.
The pH regulator is selected from one or more of triethanolamine, arginine, aminomethyl propanol, citric acid, sodium citrate, tromethamine and sodium hydroxide.
Preferably, the pH regulator is selected from triethanolamine or arginine.
The preservative is selected from one or more of imidazolidinyl urea, p-hydroxyacetophenone, methyl hydroxybenzoate, propyl hydroxybenzoate, phenoxyethanol, PE9010 (phenoxyethanol and ethylhexyl glycerol), DMDM hydantoin, sodium benzoate, caprylyl glycol and benzyl alcohol.
Preferably, the preservative is selected from one or more of methyl hydroxybenzoate, propyl hydroxybenzoate, phenoxyethanol, p-hydroxyacetophenone and PE9010 (phenoxyethanol and ethylhexyl glycerol).
The solvent is water.
The oily matrix is selected from one or more of isopropyl myristate, isopropyl palmitate, hydrogenated polydecene, caprylic/capric triglyceride, squalane, polydimethylsiloxane, lanolin, vaseline and cetostearyl alcohol.
Preferably, the oily matrix is selected from one or more of vaseline, lanolin, caprylic/capric triglyceride, squalane, cetostearyl alcohol, white oil and isopropyl myristate.
The essence is common essence in the field.
In the implementation process, the application unexpectedly finds that when the mass ratio of the caprylic acid/capric acid triglyceride, the PEG-40 hydrogenated castor oil and the anti-wrinkle composition is controlled to be 0.3:1:6, the effective ingredients in the anti-wrinkle composition can be promoted to be exerted, and the anti-wrinkle effect of the skin care product is obviously improved.
As a preferred technical scheme, the skin care product comprises the following components in percentage by weight: 0.1% of hydroxyethyl cellulose, 0.3% of xanthan gum, 4% of glycerol, 0.03% of EDTA-2Na, 0.2% of allantoin, 0.15% of methylparaben, 0.2% of p-hydroxyacetophenone, 2% of 1, 3-butanediol, 0.2% of phenoxyethanol, 0.3% of caprylic/capric triglyceride, 1% of PEG-40 hydrogenated castor oil, 6% of an anti-wrinkle composition and the balance of deionized water;
the anti-wrinkle composition is a hydrolyzed coffee abelmoschus esculentus extract and a Nicotiana benthamiana extract in a mass ratio of 1:2.
As another preferred embodiment, the skin care product comprises the following components in percentage by weight: acrylic acid (ester)/C10-30 alkanol acrylate crosspolymer 0.65%, glycerin 5%, propylene glycol 3%, triethanolamine 0.8%, PE 90100.6%, caprylic/capric triglyceride 0.3%, PEG-40 hydrogenated castor oil 1%, anti-wrinkle composition 6.5%, and the balance of deionized water;
the anti-wrinkle composition is a hydrolyzed coffee abelmoschus manihot extract and a Nicotiana benthamiana extract in a mass ratio of 1: 2.25.
Compared with the prior art, the method has the advantages that:
(1) the invention provides an anti-wrinkle composition, which takes a hydrolyzed okra extract and a bunsen extract as raw materials, and the content and the mass ratio of the hydrolyzed okra extract and the bunsen extract are controlled, so that the obtained anti-wrinkle composition can improve the water content of skin, promote the generation of type I collagen, up-regulate genes related to the generation of epidermal lipid, improve the integrity and the barrier function of the skin, increase the thickness of the epidermis, fill epidermal lipid and promote the generation of collagen and hyaluronic acid, and the two active ingredients have a synergistic effect mutually, so that the anti-wrinkle composition has the effects of more comprehensively and obviously reducing the depth and the area of wrinkles, fading wrinkles, tightening pores and improving the skin glossiness;
(2) the invention also provides a skin care product containing the anti-wrinkle composition, and in the implementation process, the weight ratio of the acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer to the anti-wrinkle composition in the thickening agent is controlled to be 1.5-6.5:30-65, and the weight ratio of the caprylic acid/capric acid triglyceride, the PEG-40 hydrogenated castor oil and the anti-wrinkle composition is controlled to be 0.3:1:6, so that the obtained skin care product can ensure the release of effective components in the anti-wrinkle composition at proper viscosity, the anti-wrinkle performance of the skin care product is improved, and the satisfaction degree of the product is improved.
Drawings
FIG. 1 effect of different anti-wrinkle compositions on type I collagen synthesis rate;
FIG. 2 reduction in wrinkle volume over time;
FIG. 3 reduction in the number of wrinkles over time;
figure 4 reduction of wrinkle depth over time.
Detailed Description
The present invention is further illustrated by the following examples, which are not intended to limit the scope of the present invention in any way.
Purchase and model of reagents used in the examples
Example 1 an anti-wrinkle composition and a method for preparing the same
The composition comprises the following components in parts by weight: 3 parts of Nicotiana benthamiana extract; 1 part of hydrolyzed coffee abelmoschus manihot extract.
The preparation method comprises the following steps: weighing the Nicotiana benthamiana extract and the Abelmoschus manihot extract according to the weight parts, and uniformly mixing to obtain the anti-wrinkle composition.
Example 2 an anti-wrinkle composition and a method for preparing the same
The composition comprises the following components in parts by weight: 2.5 parts of Nicotiana benthamiana extract; 1.5 parts of hydrolyzed coffee abelmoschus manihot extract
The preparation method comprises the following steps: weighing the Nicotiana benthamiana extract and the Abelmoschus manihot extract according to the weight parts, and uniformly mixing to obtain the anti-wrinkle composition.
Example 3 an anti-wrinkle composition and method for preparing the same
The composition comprises the following components in parts by weight: 4 parts of Nicotiana benthamiana extract; 2 parts of hydrolyzed coffee abelmoschus manihot extract
The preparation method comprises the following steps: weighing the Nicotiana benthamiana extract and the abelmoschus coffei extract according to the weight parts, and uniformly mixing to obtain the anti-wrinkle composition.
Effect test
irritation/Corrosion test-chick embryo chorioallantoic Membrane vascular test
(1) The experimental method comprises the following steps: the chick embryo chorioallantoic membrane (CAM) test is a commonly used method for evaluating the irritation of cosmetics in vitro, and utilizes the characteristic that the chorioallantoic membrane is similar to the conjunctival tissue structure of eyes in the middle stage (8-12 days) of hatching embryos. A certain amount of the test substance is dropped on the CAM, and the change of blood vessels (such as bleeding, angiolysis and blood coagulation) of the CAM is observed in a certain period of time, so that the eye irritation of the test substance is evaluated according to the damage degree of the CAM. The anti-wrinkle composition is a transparent test substance, and then is scored by adopting a reaction time method. After egg examination of the chicken embryo incubated for 10 days, marking the position of the air chamber, peeling off the eggshell part at the air chamber by using dental zigzag forceps, wetting the exposed white egg membrane by using a certain amount of 0.9% NaCl solution, and then sucking out the solution. The intima is removed with forceps and care should be taken to ensure the integrity of the vascular membrane. 0.3mL of the anti-wrinkle composition solution of the above example was directly dropped on the CAM, timed for 5min, and the change of the CAM within 5min was observed, and the initial time(s) for bleeding, clotting, and angiolysis to occur was recorded. A0.1 mol/L NaOH solution is set as a positive control, and a 0.9% NaCl solution is set as a negative control. The irritation score was calculated according to the formula (1), and the ocular irritation of the sample was graded according to the irritation score. IS < 1: no eye irritation; 1 IS < 5: mild ocular irritation; IS IS not less than 5 and less than 9: moderate ocular irritation; IS IS not less than 10: strong ocular irritation/erosion. 6 chick embryos were tested and averaged.
In the formula: sec H: bleeding time, s;
sec L: time of angiolysis, s;
sec C: clotting time, s;
(2) the experimental results are as follows: the results of the experiments are shown in table 1 below.
TABLE 1 chick embryo chorioallantoic Membrane test results
| Test article | Sec H(s) | sec L(s) | sec C(s) | IS | Category of irritation |
| Negative control (0.9% NaCl) | >300 | >300 | >300 | <1 | Has no irritation |
| Positive control (0.1mol/L NaOH) | 18 | 50 | 42 | 18.34 | Strong irritation/corrosion |
| Anti-wrinkle composition of example 1 | >300 | >300 | >300 | <1 | Has no irritation |
| Anti-wrinkle composition of example 2 | >300 | >300 | >300 | <1 | Has no irritation |
| Anti-wrinkle composition of example 3 | >300 | >300 | >300 | <1 | Has no irritation |
As can be seen from the data in table 1, the anti-wrinkle compositions prepared in examples 1 to 3 above were non-irritating and safe to use.
Application example 1 anti-wrinkle cream and preparation method thereof
Comprises the following components:
the preparation method comprises the following steps:
(1) taking acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer with the formula dosage, adding deionized water, heating, soaking uniformly, homogenizing, dispersing uniformly, then sequentially adding glycerol, EDTA-2Na, propylene glycol and Tween 60 with the formula dosage, starting heating, and heating to 80-85 ℃ to obtain phase A;
(2) weighing vaseline, caprylic/capric triglyceride, glyceryl monostearate, span 60, lanolin, 26# white oil, cetostearyl alcohol and propyl hydroxybenzoate according to formula dosage in sequence, heating to 80-85 deg.C, and dissolving uniformly to obtain phase B;
(3) adding the phase B into the phase A, keeping the temperature, starting 10000rpm, and homogenizing for 3min to obtain a mixture 1;
(4) adding triethanolamine into the mixture 1 when the temperature of the mixture is reduced to 60 ℃, and uniformly stirring to obtain a mixture 2;
(5) when the temperature of the mixture is reduced to 40-45 ℃, sequentially adding phenoxyethanol and the anti-wrinkle composition, and uniformly stirring to obtain the anti-wrinkle cream.
Application comparative examples 1-3 anti-wrinkle cream and preparation method thereof
Comprises the following components:
the preparation method is the same as that of application example 1.
Application example 2 anti-wrinkle water and preparation method thereof
Comprises the following components:
the preparation method comprises the following steps:
(1) weighing p-hydroxyacetophenone and methyl propylene glycol according to the formula dosage, mixing, and dissolving to obtain a phase B;
(2) weighing glycerol dispersed xanthan gum with a formula dosage, adding deionized water to dissolve, sequentially adding EDTA-2Na, 1, 3-butanediol and hexanediol, starting heating, adding phase B when the temperature reaches 70-75 ℃, continuously heating to about 85 ℃ to ensure complete dissolution, and obtaining a mixture 1;
(3) when the temperature of the mixture 1 is reduced to 45 ℃, the caprylic/capric triglyceride, the PEG-40 hydrogenated castor oil and the anti-wrinkle composition are sequentially added, and the mixture is stirred uniformly to obtain the anti-wrinkle water.
Application comparative example 4 anti-wrinkle water and preparation method thereof
Comprises the following components:
the preparation method is the same as that of application example 2.
Application example 3 anti-wrinkle emulsion and preparation method thereof
Comprises the following components:
the preparation method comprises the following steps:
(1) weighing p-hydroxyacetophenone and methyl propylene glycol according to the formula dosage, mixing, and dissolving to obtain an A1 phase;
(2) weighing acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer with a formula dosage, heating and soaking the acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer with deionized water uniformly, homogenizing and dispersing the heated and soaked polymer uniformly, adding xanthan gum pre-dispersed by glycerol, sequentially adding EDTA-2Na and 1, 3-butanediol, starting heating, heating to 70-75 ℃, adding a pre-dissolved transparent A1 phase, and continuously heating to 80-85 ℃ to obtain an A phase;
(3) weighing glyceryl monostearate, PEG-100 glyceryl stearate, isopropyl myristate, caprylic/capric triglyceride, cetostearyl alcohol and vaseline according to formula dosage, heating to 80-85 deg.C, and dissolving uniformly to obtain phase B;
(4) adding the phase B into the phase A, keeping the temperature, starting 10000rpm, and homogenizing for 3min to obtain a mixture 1;
(5) cooling the mixture 1 to 60 ℃, adding triethanolamine, and uniformly stirring to obtain a mixture 2;
(6) when the temperature of the mixture 2 is reduced to 40-45 ℃, sequentially adding phenoxyethanol and the anti-wrinkle composition, and uniformly stirring to obtain the anti-wrinkle emulsion.
Application comparative example 5 anti-wrinkle emulsion and preparation method thereof
Comprises the following components:
the preparation method is the same as that of application example 3.
Application example 4 anti-wrinkle essence and preparation method thereof
Comprises the following components:
the preparation method comprises the following steps:
(1) weighing p-hydroxyacetophenone and methyl propylene glycol according to the formula dosage, mixing, and dissolving to obtain an A1 phase;
(2) weighing acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer with a formula dosage, heating and soaking the acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer with deionized water uniformly, homogenizing and dispersing the heated and soaked polymer uniformly, then adding carboxymethyl cellulose pre-dispersed by glycerol, sequentially adding EDTA-2Na and methyl propylene glycol, starting heating, heating to 70-75 ℃, adding a pre-dissolved transparent A1 phase, and continuing heating to 80-85 ℃ to obtain a phase A;
(3) weighing squalane, isopropyl myristate and caprylic/capric triglyceride according to formula dosage, heating to 80-85 deg.C, and dissolving uniformly to obtain phase B;
(4) adding the phase B into the phase A, keeping the temperature, starting 10000rpm, and homogenizing for 3min to obtain a mixture 1;
(5) when the temperature of the mixture 1 is reduced to 60 ℃, adding arginine and deionized water according to the formula dosage, and uniformly stirring to obtain a mixture 2;
(6) and (3) when the temperature of the mixture 2 is reduced to 40-45 ℃, sequentially adding the PE9010 and the anti-wrinkle composition, and uniformly mixing to obtain the anti-wrinkle essence.
Application comparative example 6 anti-wrinkle essence and preparation method thereof
Comprises the following components:
the preparation method is the same as in application example 4.
Application example 5 an anti-wrinkle mask and a preparation method thereof
Comprises the following components:
the preparation method comprises the following steps:
(1) weighing p-hydroxyacetophenone and 1, 3-butanediol with the formula dosage, mixing, and dissolving to obtain a phase B;
(2) taking glycerol with a formula dosage, pre-dispersing hydroxyethyl cellulose, sequentially adding xanthan gum, EDTA-2Na, allantoin, methylparaben and deionized water, starting heating, adding a pre-dissolved transparent B-phase raw material when heating to 70-75 ℃, and then heating to 80-85 ℃ for uniform dissolution to obtain a mixture 1;
(3) weighing caprylic acid/capric acid triglyceride and PEG-40 hydrogenated castor oil according to the formula dosage, and uniformly mixing to obtain a phase D;
(4) and (3) when the temperature of the mixture 1 is reduced to 40-45 ℃, adding phenoxyethanol, the uniformly premixed phase D and the anti-wrinkle composition, and uniformly stirring to obtain the anti-wrinkle mask.
Application comparative examples 7-10 anti-wrinkle mask and preparation method thereof
Comprises the following components:
the preparation method is the same as in application example 5.
Application example 6 anti-wrinkle gel and preparation method thereof
Comprises the following components:
the preparation method comprises the following steps:
(1) weighing acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer with formula dosage, heating and soaking with deionized water uniformly, homogenizing and dispersing uniformly, adding glycerol and propylene glycol, starting heating, and heating to 80-85 ℃ to obtain phase A;
(2) adding triethanolamine when the temperature of the phase A is reduced to 60 ℃, and uniformly stirring to obtain a mixture 1;
(3) weighing caprylic acid/capric acid triglyceride and PEG-40 hydrogenated castor oil according to the formula dosage, and uniformly mixing to obtain a phase D;
(3) and (3) when the temperature of the mixture 1 is reduced to 40-45 ℃, adding PE9010, the uniformly premixed phase D and the anti-wrinkle composition, and uniformly stirring to obtain the anti-wrinkle gel.
Application comparative example 11 of anti-wrinkle gel and preparation method thereof
Comprises the following components:
the preparation method is the same as that of application example 6.
Effect test
1. Fibroblast protein expression assay
(1) Test samples: the experimental group is the anti-wrinkle skin care product of application examples 1-6
(2) The test method comprises the following steps: the human skin fibroblasts after recovery were subcultured in DMEM medium containing 10% Fetal Bovine Serum (FBS), with 1 change of the culture medium every 2d, to logarithmic phase. Then taking the fibroblasts in the logarithmic phase for suspension culture, adjusting the cell density (the cell density is 111111/mL and 90 muL of the cells are added into a 96-well plate in each well in the cell viability detection process, the cell density is 30000/mL and 2mL of the cells are added into a 12-well plate in each well in the I type collagen synthesis detection process), the temperature is 37 ℃ and the content of 5% CO is 5% 2 Culturing under the condition. After 24h, the cells were attached and the test sample and positive control (EGF, 10ng/mL) were added. The supernatants were harvested and tested for type I collagen content using an ELISA kit, with the cell number and the amount of collagen produced by untreated cells as references (═ 100%).
(3) And (3) test results: the results are shown in detail in FIG. 1.
According to the detection data in fig. 1, it can be seen that the anti-wrinkle skin care products prepared in examples 1 to 6 can better promote the synthesis of type I collagen, and especially the skin care products prepared in examples 5 to 6 have higher efficiency in promoting the synthesis of type I collagen.
2. Skin elasticity test
2.1 test sample: the experimental groups were the products of examples 1-6 and comparative examples 1-11.
2.2 test methods: volunteers 85 (age 28-45 years) were randomly grouped into 5 persons each, and the left and right cheekbones and cheeks of the face were used as test areas in the experiment, and the test volunteers applied 2g of sample on the face every time, and applied the test article twice a day in the morning and at night for a total of 28 days. During the experiment, the subject was not able to apply any other cosmetic product at the experimental site. The skin elasticity changes of the test areas of the day 0, the day 14 and the day 28 are respectively tested by using a skin elasticity tester Cutomer MPA580, the probe repeatedly measures the skin elasticity changes in the same test area for 3 times during measurement, the average value is obtained, and the test of the same volunteer is completed by the same measurer.
2.3 testing the instrument: skin elasticity tester Cutometer dual MPA580, CK company, germany.
2.4 test results: the skin elasticity test results are detailed in tables 2-4 below.
TABLE 2 test results of application example 1 and application comparative examples 1 to 4
The difference between application comparative example 1 and application example 1 is that: the anti-wrinkle composition does not contain hydrolyzed okra extract, and other components and contents are not changed;
the difference between application comparative example 2 and application example 1 is that: replacing the anti-wrinkle composition with retinol, wherein other components and contents are not changed;
the difference between application comparative example 3 and application example 1 is that: the anti-wrinkle composition is not added with the Nicotiana benthamiana extract, and other components and contents are not changed;
and (4) conclusion:
the skin elasticity value of the hydrolyzed coffee abelmoschus manihot extract and the nicotiana benthamiana extract which are added into a skin care product formula independently or in a compounding manner can be increased, so that the skin elasticity of the hydrolyzed coffee abelmoschus manihot extract and the nicotiana benthamiana extract can be improved, the skin elasticity increasing value of the embodiment in which the hydrolyzed coffee abelmoschus manihot extract and the nicotiana benthamiana extract are compounded is superior to that of the comparative example in which the hydrolyzed coffee abelmoschus manihot extract and the nicotiana benthamiana extract are added independently or not, and the hydrolyzed coffee abelmoschus manihot extract and the nicotiana benthamiana extract have a synergistic effect on the aspect of increasing the skin elasticity.
TABLE 3 test results of application examples 2 to 4 and 6 and application comparative examples 4 to 6 and 11
Application examples 2 to 4, 6 and application comparative examples 4 to 6, 11 are different in that the anti-wrinkle composition is replaced with retinol.
And (4) conclusion: from application examples 2-4, 6 and application comparative examples 4-6, 11, it can be seen that retinol and the anti-wrinkle composition can improve skin elasticity after 28 days of use, and the examples of the anti-wrinkle composition are equivalent to or better than the comparative examples of retinol.
TABLE 4 test results of application example 5 and application comparative examples 7 to 10
The difference between application comparative example 7 and application example 5 is that: replacing the anti-wrinkle composition with retinol, and leaving other components and contents unchanged;
the difference between application comparative example 8 and application example 5 is that: caprylic/capric triglyceride is not added, 1.3% of PEG-40 hydrogenated castor oil, and other components and contents are not changed;
comparative application example 9 differs from application example 5 in that: PEG-40 hydrogenated castor oil is not added, caprylic/capric triglyceride is 1.3%, and other components and contents are not changed;
comparative application example 10 differs from application example 1 in that: changing the mass ratio of caprylic/capric triglyceride to PEG-40 hydrogenated castor oil to be 1:1, other components and contents were not changed.
And (4) conclusion: according to the data in table 4, it can be seen that the anti-wrinkle mask pack obtained by using the components disclosed in the present invention and controlling the mass ratio thereof within the range of the present invention has a better effect of improving skin elasticity.
3. Anti-wrinkle efficacy assessment
3.1 test sample: the experimental groups were the products of examples 1-6 and comparative examples 1-12.
3.2 test method: volunteers 90 (ages 35-50) had marked fine lines or wrinkles on their faces, randomized into 18 groups. In the experiment, the canthus tail line area is a test area, and the canthus position (the dosage is 0.1g) is coated by using the embodiment in the experiment period for 2 times per day, 1 time respectively in the morning and at night and continuously used for 8 weeks; volunteers followed 1 time each at week 4 and 8 of use, each at the same time of day. The PROMIS system analysis was used clinically to assess the percent reduction in tail-eye wrinkle depth, wrinkle volume, number of wrinkles over time.
3.1 test results: the skin anti-wrinkle efficacy evaluation test results are shown in tables 5-7 below and figures 2-4.
TABLE 5 test results of application example 1 and application comparative examples 1 to 3
TABLE 6 test results of application examples 2 to 4 and 6 and application comparative examples 4 to 6 and 11
TABLE 7 examination results of application example 5 and application comparative examples 7 to 10
And (4) conclusion: according to the data of the inspection sides in the above tables 5-7, the anti-wrinkle effect of the product prepared by the invention is better, and when the content or the mass ratio of the changed components is not in the protection range of the invention, the anti-wrinkle effect of the obtained product is obviously reduced.
The test results show that: the hydrolyzed coffee abelmoschus manihot extract and the nicotiana benthamiana extract are added into a skin care product formula independently or in a compounding way, so that the quantity, the depth and the volume of wrinkles are reduced, the hydrolyzed coffee abelmoschus manihot extract and the nicotiana benthamiana extract can resist wrinkles and remove wrinkles, the quantity, the depth and the volume reduction of the wrinkles of a compound example of the hydrolyzed coffee abelmoschus manihot extract and the nicotiana benthamiana extract are better than those of a comparative example of the hydrolyzed coffee abelmoschus manihot extract and the nicotiana benthamiana extract which are added or not added independently, and the hydrolyzed coffee abelmoschus manihot extract and the nicotiana benthamiana extract have mutual synergistic effect on the reduction of the quantity, the depth and the volume of the wrinkles.
4. Human testing-consumer self assessment
4.1 scoring criteria: the score 1 was unsatisfactory, the score 2 was less satisfactory, the score 3 was general, the score 4 was satisfactory, and the score 5 was very satisfactory.
4.2 test sample: the product groups of application examples 1,3, 4 and application comparative example 7 and the blank group after removal of the anti-wrinkle composition in application example 1.
4.3 test methods: the product and blank groups were scored for 4 and 8 weeks of product use according to scoring criteria.
4.4 test results: the calculation method comprises the following steps: satisfaction ÷ subjects with a score greater than 3 ÷ total population × 100%, self-assessment results are shown in table 8 below.
TABLE 8 self-assessment results
From the above scoring results it can be seen that: after 8 weeks of use, the overall satisfaction of the product group is higher than 4, while the overall satisfaction of the blank group is only 3.3, 91% of volunteers are satisfied with the product, and the anti-wrinkle composition is good in anti-wrinkle effect and high in satisfaction of the volunteers.
The foregoing is merely a preferred embodiment of the invention and is not intended to limit the invention in any manner; as will be apparent to those skilled in the art in view of the foregoing description, there is provided the present invention; however, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the scope of the invention; meanwhile, any equivalent changes, modifications and evolutions made to the above embodiments according to the substantial technology of the present invention are still within the protection scope of the technical solution of the present invention.
Claims (5)
1. A skin care product characterized by: the skin care product comprises an anti-wrinkle composition and an auxiliary agent;
the anti-wrinkle composition comprises the following components in parts by weight: 1-20 parts of Nicotiana benthamiana extract; 0.5-15 parts of hydrolyzed coffee abelmoschus manihot extract; the mass ratio of the Nicotiana benthamiana extract to the hydrolyzed coffee abelmoschus manihot extract is 1-5: 1;
the auxiliary agent comprises a chelating agent, a thickening agent, a humectant, a solubilizer, a preservative, a solvent, a pH regulator and an oily matrix;
the thickening agent is selected from one or more of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, xanthan gum, carboxymethyl cellulose and hydroxyethyl cellulose;
the oily matrix is caprylic/capric triglyceride;
the solubilizer is PEG-40 hydrogenated castor oil;
the mass ratio of the acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer to the anti-wrinkle composition is 1.5-6.5: 30-65;
the mass ratio of the caprylic acid/capric acid triglyceride, the PEG-40 hydrogenated castor oil and the anti-wrinkle composition is 0.3:1: 6.
2. The skin care product of claim 1, characterized in that: the anti-wrinkle composition comprises the following components in parts by weight: 10-15 parts of Nicotiana benthamiana extract; 5-10 parts of hydrolyzed coffee abelmoschus manihot extract.
3. The skin care product of claim 1, characterized in that: the preparation method of the anti-wrinkle composition comprises the following steps: weighing the benschniakia tobacco extract and the hydrolyzed coffee abelmoschus manihot extract according to the formula dosage, and uniformly stirring to obtain the anti-wrinkle composition.
4. The skin care product of claim 1, characterized in that: the skin care product is water, emulsion, cream, essence, facial mask or gel.
5. The skin care product of claim 1, characterized in that: the thickening agent is selected from acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer.
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| CN110115699A (en) * | 2019-04-16 | 2019-08-13 | 上海悦目化妆品有限公司 | A kind of eye essence and preparation method thereof |
| CN113631566A (en) * | 2019-02-08 | 2021-11-09 | 路博润先进材料公司 | Compounds useful for the treatment and/or care of the skin, hair, nails and/or mucous membranes |
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| CN113631566A (en) * | 2019-02-08 | 2021-11-09 | 路博润先进材料公司 | Compounds useful for the treatment and/or care of the skin, hair, nails and/or mucous membranes |
| CN110115699A (en) * | 2019-04-16 | 2019-08-13 | 上海悦目化妆品有限公司 | A kind of eye essence and preparation method thereof |
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