CN114096660A - 高组织再生能力的软骨细胞培养物 - Google Patents

高组织再生能力的软骨细胞培养物 Download PDF

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Publication number
CN114096660A
CN114096660A CN202080034375.3A CN202080034375A CN114096660A CN 114096660 A CN114096660 A CN 114096660A CN 202080034375 A CN202080034375 A CN 202080034375A CN 114096660 A CN114096660 A CN 114096660A
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China
Prior art keywords
culture
tissue
tgp
cartilage tissue
chondrocyte
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Pending
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CN202080034375.3A
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English (en)
Chinese (zh)
Inventor
加藤正二郎
S·Jk·亚伯拉罕
洼田倭
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Jbm Co ltd
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Jbm Co ltd
Guided Knowledge Ip Pty Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/65—MicroRNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/905—Hyaluronic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2537/00—Supports and/or coatings for cell culture characterised by physical or chemical treatment
    • C12N2537/10—Cross-linking
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2539/00—Supports and/or coatings for cell culture characterised by properties
    • C12N2539/10—Coating allowing for selective detachment of cells, e.g. thermoreactive coating

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
CN202080034375.3A 2019-04-08 2020-04-08 高组织再生能力的软骨细胞培养物 Pending CN114096660A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019073783 2019-04-08
JP2019-073783 2019-04-08
PCT/JP2020/015896 WO2020209316A1 (ja) 2019-04-08 2020-04-08 組織再生能が高い軟骨細胞培養物

Publications (1)

Publication Number Publication Date
CN114096660A true CN114096660A (zh) 2022-02-25

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CN202080034375.3A Pending CN114096660A (zh) 2019-04-08 2020-04-08 高组织再生能力的软骨细胞培养物

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US (1) US20230092155A1 (https=)
EP (1) EP3954761A4 (https=)
JP (1) JP7578958B2 (https=)
CN (1) CN114096660A (https=)
WO (1) WO2020209316A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2022065328A1 (https=) * 2020-09-23 2022-03-31

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6103528A (en) * 1998-04-17 2000-08-15 Battelle Memorial Institute Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices
US20040203148A1 (en) * 2001-07-13 2004-10-14 Hiroshi Yoshioka Support for cell/tissue culture and culture method
CN1897989A (zh) * 2003-11-04 2007-01-17 国家健康医学研究所 水凝胶用于培养软骨细胞的用途
WO2007035778A2 (en) * 2005-09-19 2007-03-29 Histogenics Corporation Cell-support matrix and a method for preparation thereof
CN106350482A (zh) * 2016-09-30 2017-01-25 广州赛莱拉干细胞科技股份有限公司 一种培养体系及其应用与培养软骨细胞的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995024430A2 (en) * 1994-03-04 1995-09-14 University Of Washington Block and graft copolymers and methods relating thereto
US7537780B2 (en) * 2002-03-22 2009-05-26 Histogenics Corporation Method for preparing and implanting a cartilage construct to treat cartilage lesions
JPWO2004074463A1 (ja) * 2003-02-19 2006-06-01 学校法人早稲田大学 細胞分離用ハイドロゲルおよび細胞の分離方法
WO2016006712A1 (ja) 2014-07-11 2016-01-14 国立研究開発法人産業技術総合研究所 細胞分化ポテンシャル判別法
JPWO2018154813A1 (ja) * 2017-02-24 2019-02-28 株式会社セルシード 組織再生培養細胞シート、製造方法及びその利用方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6103528A (en) * 1998-04-17 2000-08-15 Battelle Memorial Institute Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices
US20040203148A1 (en) * 2001-07-13 2004-10-14 Hiroshi Yoshioka Support for cell/tissue culture and culture method
CN1897989A (zh) * 2003-11-04 2007-01-17 国家健康医学研究所 水凝胶用于培养软骨细胞的用途
WO2007035778A2 (en) * 2005-09-19 2007-03-29 Histogenics Corporation Cell-support matrix and a method for preparation thereof
CN106350482A (zh) * 2016-09-30 2017-01-25 广州赛莱拉干细胞科技股份有限公司 一种培养体系及其应用与培养软骨细胞的方法

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Publication number Publication date
WO2020209316A9 (ja) 2021-09-16
WO2020209316A1 (ja) 2020-10-15
JP7578958B2 (ja) 2024-11-07
EP3954761A1 (en) 2022-02-16
US20230092155A1 (en) 2023-03-23
JPWO2020209316A1 (https=) 2020-10-15
EP3954761A4 (en) 2023-05-17

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