CN114088892A - Potato root system in-situ observation test device - Google Patents

Potato root system in-situ observation test device Download PDF

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Publication number
CN114088892A
CN114088892A CN202111355861.8A CN202111355861A CN114088892A CN 114088892 A CN114088892 A CN 114088892A CN 202111355861 A CN202111355861 A CN 202111355861A CN 114088892 A CN114088892 A CN 114088892A
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China
Prior art keywords
potato
box
test box
root system
test
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Pending
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CN202111355861.8A
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Chinese (zh)
Inventor
吕钊彦
张辉
黄月
杨鑫
白拯
刘熏遥
邵雨晴
王碧晨
喻尚艺
侯华兰
朱晓彪
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Anhui Agricultural University AHAU
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Anhui Agricultural University AHAU
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Priority to CN202111355861.8A priority Critical patent/CN114088892A/en
Publication of CN114088892A publication Critical patent/CN114088892A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02Food
    • G01N33/025Fruits or vegetables

Abstract

The invention discloses a potato root system in-situ observation test device which comprises a test box, at least one layered grid plate with different apertures, a nutrient solution layering system and a fixing mechanism, wherein the layered grid plate is arranged in the test box, and the fixing mechanism is arranged at the top of the test box and is used for fixing potato plants. The invention is beneficial to researching the in-situ growth state of the potato root system and the test problem of in-situ expansion of different layered tubers.

Description

Potato root system in-situ observation test device
Technical Field
The invention relates to the technical field of potato planting, in particular to an in-situ observation test device for a potato root system.
Background
At present, when researching the root stress resistance or growth mode of potato crops, the commonly used test methods comprise a solution culture method, a pot culture method and a field test. Due to the spatial and temporal differences of the soil, potting and field experiments are difficult to control other conditions as accurately and effectively as hydroponic experiments. The root system cultured by the solution can be directly contacted with the nutrient solution, the rapid screening of the large-batch genotypes can be carried out according to certain morphological or physiological indexes, the culture condition can be accurately repeated, and meanwhile, the character characteristics of the root system which are difficult to observe in the field test can be better observed. Therefore, the solution culture method becomes the first choice for root system related experiments.
However, the existing root system hydroponics device has the following disadvantages: 1. the potato tubers cultured by atomization have rough skins, large pores and easy infection of diseases; 2. the shallow layer cultivation is easy to rot in the expansion period of potato tubers, so that the root system grows slowly, becomes white and rot continuously, and nutrient solution is polluted, so that the diseases spread willingly; 3. the traditional root system culture device cannot observe the in-situ growth condition and configuration of the root system; 4. the domestic device generally has large nutrient solution demand in the test process, more replacement times and lower utilization efficiency.
Disclosure of Invention
The invention aims to solve the technical problem of providing a potato root system in-situ observation test device for researching the in-situ growth condition of a potato root system.
In order to solve the technical problems, the invention adopts the following technical scheme: the utility model provides a potato root system in situ observation test device, includes the proof box, sets up at least one net board in the proof box and set up the fixed establishment that is used for fixed potato plant at the top of proof box.
Furthermore, the grid plates are arranged in the test box at intervals from top to bottom, and the through holes of the grid plates are gradually reduced from top to bottom.
Further, a plurality of grid plates are arranged in the test box at intervals from top to bottom by adopting the following structure:
both sides of the grid plate are respectively hinged with more than one supporting rod, and the other ends of the more than one supporting rods are respectively hinged with the test box.
Further, still include with a plurality of the net board one-to-one be used for adjusting a plurality of adjusting part of net board height, adjusting part includes connecting rod and slider, seted up the arc spout on the proof box, the one end of connecting rod is passed the arc spout sets up in the proof box and with corresponding arbitrary on the net board the one end of bracing piece is connected, the other end setting is in outside the proof box with slider sliding connection, be provided with more than one lug on the slider, the proof box is corresponding the position department of arc spout seted up with more than one the more than one recess of lug complex.
Furthermore, the adjusting assembly further comprises more than one telescopic rod, one end of each telescopic rod is hinged with the grid plate, and the other end of each telescopic rod is hinged with the inner wall of the test box.
Furthermore, the fixing mechanism comprises two box covers respectively hinged to the test box and two clamping assemblies respectively arranged on the two box covers, and the two clamping assemblies can be spliced to clamp and fix the potato plants.
Further, two the centre gripping subassembly structure is the same, all includes the limiting plate, slides and sets up clamp splice on the limiting plate and will the clamp splice with limiting plate connected's spring, set up jaggedly on the case lid, the limiting plate sets up the case lid is corresponding the position department of breach.
Furthermore, the test box is made of transparent materials, grid lines are arranged outside any two adjacent side faces of the test box, and the grid lines become dense gradually from top to bottom.
Furthermore, still include the nutrient solution case, it is a plurality of the inside of grid plate has all been seted up a plurality of capillary, the top all seted up with a plurality of apopores that the capillary communicates, and is a plurality of the grid plate all with the nutrient solution case communicates.
A potato test method adopts a potato root system in-situ observation test device, and comprises the following operation steps:
s1, placing the potato tubers in a humid environment at the temperature of between 15 and 30 ℃ for 3 to 5 days continuously until the potato tubers sprout to form 8 to 10 cm plants;
s2, planting the germinated potato plants in a test box, fixing the leaves of the potatoes outside the test box through a fixing mechanism, clamping the roots of the potatoes in the test box, and placing the test box in a cool environment to avoid the potatoes from being exposed to light, and moving the potatoes into an astigmatic environment after waiting for about one week;
s3, observing the growth condition of the roots of the potato plants in the test box every 3 days, and shooting photos of the roots of the potato plants in the test box by a camera for subsequent observation;
s4, observing the root conditions of the potato plants in the shooting test box at different periods, observing the thickness and density of the roots and the number and size of the potato tubers, and making statistical data into a table.
The invention has the beneficial effects that:
the potato root system in-situ observation test device is provided with the test box, the fixing mechanism is used for fixing the stems of the potato plants, the root systems of the potato plants are fixed in the test box through the fixing mechanism, the root systems of the potato plants are divided into the stolons and the potato tubers growing layer, the growing root growing layer and the absorption root growing layer with smaller root diameter through the grid plate, and the stolons and the potato tubers growing layer can not be directly contacted with nutrient solution through different layer growing modes, so that the stolons and the potato tubers growing layer are not easy to rot in the potato tuber growing period, and the normal growth and observation test of the potato plants are facilitated.
Drawings
FIG. 1 is a schematic structural view of a potato root system in-situ observation test device of the invention;
FIG. 2 is an enlarged view of portion A of FIG. 1;
FIG. 3 is a sectional view of the structure of the potato root system in-situ observation test device of the present invention;
FIG. 4 is an enlarged view of portion B of FIG. 3;
FIG. 5 is a top view of the securing mechanism of the present invention;
FIG. 6 is a schematic view of the through holes of different grid plates according to the present invention;
FIG. 7 is a schematic diagram of the grid line locations of the present invention.
The components in the drawings are labeled as follows: 1. a test chamber; 101. an arc-shaped chute; 102. a groove; 103. grid lines; 2. a grid plate; 201. a water outlet hole; 3. a fixing mechanism; 301. a box cover; 3011. a notch; 302. a clamping assembly; 3021. a limiting plate; 3022. a clamping block; 3023. a spring; 4. a support bar; 5. an adjustment assembly; 501. a connecting rod; 502. a slider; 5021. a bump; 503. a telescopic rod; 6. nutrient solution box.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The embodiments and features of the embodiments in the present application may be combined with each other without conflict. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that if directional indications such as up, down, left, right, front, and rear … … are involved in the embodiment of the present invention, the directional indications are only used to explain the relative positional relationship, motion, and the like between the components in a specific posture as shown in the drawings, and if the specific posture is changed, the directional indications are changed accordingly.
In addition, if there is a description of "first", "second", etc. in an embodiment of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, the meaning of "and/or" appearing throughout includes three juxtapositions, exemplified by "A and/or B" including either A or B or both A and B. In addition, "a plurality" means two or more. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.
See fig. 1-4.
The invention discloses a potato root system in-situ observation test device, which comprises a test box 1, at least one grid plate 2 arranged in the test box 1 and a fixing mechanism 3 arranged at the top of the test box 1 and used for fixing potato plants.
The potato root system in-situ observation test device is provided with the test box 1, the fixing mechanism 3 is used for fixing the stems of the potato plants, the root systems of the potato plants are fixed in the test box through the fixing mechanism 3, and the grid plates 2 can layer the root systems of the potato plants, so that the in-situ growth state of the potato root systems can be observed conveniently.
The potato test method adopts the potato root system in-situ observation test device, and comprises the following operation steps:
s1, placing the potato tubers in a humid environment at the temperature of between 15 and 30 ℃ for 3 to 5 days continuously until the potato tubers sprout to form 8 to 10 cm plants;
s2, planting the germinated potato plants in a test box 1, fixing the leaves of the potatoes outside the test box 1 through a fixing mechanism 3, clamping the roots of the potatoes in the test box 1, placing the test box 1 in a cool environment, avoiding the potatoes from contacting with light, and moving the potatoes into an astigmatic environment within about one week;
s3, observing the growth condition of the roots of the potato plants in the test box 1 every 3 days, and shooting photos of the roots of the potato plants in the test box 1 by a camera for subsequent observation;
s4, observing the root conditions of the potato plants in the shooting test box 1 at different periods, observing the thickness and density of the roots and the number and size of the potato tubers, and making statistical data into a table.
In an embodiment, referring to fig. 3 and 6, a plurality of grid plates 2 are arranged in the test chamber 1 at intervals from top to bottom, and the through holes of the plurality of grid plates 2 become smaller from top to bottom. Design like this, the root system that accords with the potato is along with the characteristics of the gradual extension densification of growth, with the proper degree layering of root system of potato, the follow-up observation of being convenient for, in this embodiment, the grid plate 2 that sets up has three, can divide into the root system of potato three-layer, and three-layer from the top down is stolon and potato tuber growing layer, growth root growing layer respectively in proper order and is used for the absorption root growing layer that the root diameter is more little.
In one embodiment, referring to fig. 3 and 6, a plurality of the grid plates 2 are arranged in the test chamber 1 at intervals from top to bottom in the following structure:
two sides of the grid plate 2 are respectively hinged with more than one supporting rod 4, and the other ends of the more than one supporting rods 4 are respectively hinged with the test box 1. Design like this for the distance between a plurality of net boards 2 is adjustable, and when the in-process at potato plant growth, it is intensive to observe and discover root system between each net board 2, is not convenient for observe, can be through corresponding adjusting part 5, adjusts the interval between net board 2.
In an embodiment, referring to fig. 1 to 4, the test device further includes a plurality of adjusting assemblies 5 corresponding to the plurality of grid plates 2 one to one and used for adjusting heights of the plurality of grid plates 2, each adjusting assembly 5 includes a connecting rod 501 and a sliding block 502, an arc-shaped chute 101 is formed in the test box 1, one end of each connecting rod 501 passes through the arc-shaped chute 101 and is arranged in the test box 1 and connected to one end of any one of the supporting rods 4 on the corresponding grid plate 2, the other end of each connecting rod 501 is arranged outside the test box 1 and slidably connected to the corresponding sliding block 502, one or more protrusions 5021 are arranged on the corresponding sliding block 502, and one or more grooves 102 matched with one or more protrusions 5021 are formed in positions of the test box 1 corresponding to the arc-shaped chutes 101. Design like this, realize the distance regulatory function between a plurality of net boards 2, only need during the regulation to test box 1 outside direction slip slider 502, take out lug 5021 and leave corresponding recess 102 and can remove connecting rod 501, connecting rod 501 is used for promoting or reduces the position of net board 2, fixed only need to test box 1 inboard direction slip slider 502, insert corresponding recess 102 with lug 5021 can.
In an embodiment, referring to fig. 3, the adjusting assembly 5 further includes more than one telescopic rod 503, one end of each of the more than one telescopic rods 503 is hinged to the grid plate 2, and the other end of each of the more than one telescopic rods 503 is hinged to the inner wall of the test chamber 1. By the design, the stability of the grid plate 2 in the test box 1 is ensured to a certain extent.
In one embodiment, referring to fig. 5, the fixing mechanism 3 includes two covers 301 hinged to the test chamber 1, and two clamping assemblies 302 disposed on the two covers 301, and the two clamping assemblies 302 can clamp and fix potato plants. By the design, the potato plants can be fixed and prevented from being toppled.
In an embodiment, referring to fig. 5, the two clamping assemblies 302 have the same structure, and each clamping assembly includes a stop plate 3021, a clamp block 3022 slidably disposed on the stop plate 3021, and a spring 3023 connecting the clamp block 3022 and the stop plate 3021, the cover 301 has a notch 3011, and the stop plate 3021 is disposed on the cover 301 at a position corresponding to the notch 3011. Design like this, the fixed potato plant of anchor clamps 3022 concatenation centre gripping through two fixed establishment 3, the elastic action of anchor clamps 3022 and spring 3023 can realize when the centre gripping potato plant that can carry out the self-regulation along with the potato plant grows up simultaneously, in this embodiment, the vertical setting of limiting plate 3021 of setting is on case lid 301 and is the C type structure, and anchor clamps 3022 slides and sets up in C type open-ended inboard.
In an embodiment, referring to fig. 7, the test chamber 1 is made of a transparent material, grid lines 103 are arranged outside any two adjacent side surfaces of the test chamber 1, and the grid lines 103 are gradually densified from top to bottom. The grid lines 103 are convenient for follow-up experimental operation to observe the roots of the potato plants, and observe the thickness and density of the roots and the number and size of the potato tubers of the same grid in different periods, and the density of the grid lines 103 is in accordance with the characteristic that the potato roots gradually become thinner and smaller from top to bottom, so that better observation is facilitated.
In an embodiment, referring to fig. 1 and 6, the nutrient solution tank 6 is further included, a plurality of capillary holes are formed in each of the plurality of grid plates 2, a plurality of water outlet holes 201 communicated with the plurality of capillary holes are formed in the tops of the plurality of grid plates, and the plurality of grid plates 2 are communicated with the nutrient solution tank 6. Design like this, make the nutrient solution in the nutrient solution case 6 flow in the capillary hole and the apopore 201 of different grid plate 2 through capillary action, let the potato plant can absorb the nutrient solution through apopore 201 in the growth process, need not frequently change the nutrient solution, and make stolon and potato tuber not direct contact nutrient solution lead to rotting, reduce experimental error, make things convenient for accurate control test condition.
It should be understood that the examples and embodiments described herein are for illustrative purposes only and are not intended to limit the scope of the present disclosure, and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this disclosure.

Claims (10)

1. The utility model provides a potato root system in situ observation test device which characterized in that includes proof box (1), sets up different aperture layer-stepping grid board (2) of at least one in proof box (1) and sets up be used for fixed potato plant's fixed establishment (3) at the top of proof box (1).
2. The potato root system in-situ observation test device according to claim 1, wherein the grid plates (2) are arranged in the test box (1) at intervals from top to bottom, and the through holes of the grid plates (2) are gradually reduced from top to bottom.
3. The potato root system in-situ observation test device according to claim 2, wherein a plurality of grid plates (2) are arranged in the test box (1) at intervals from top to bottom by adopting the following structure:
both sides of the grid plate (2) are respectively hinged with more than one supporting rod (4), and the other ends of the more than one supporting rods (4) are respectively hinged with the test box (1).
4. The potato root system in-situ observation test device according to claim 3, further comprising a plurality of adjusting assemblies (5) corresponding to the grid plates (2) one to one and used for adjusting the heights of the grid plates (2), wherein each adjusting assembly (5) comprises a connecting rod (501) and a sliding block (502), an arc-shaped sliding groove (101) is formed in the test box (1), one end of each connecting rod (501) penetrates through the arc-shaped sliding groove (101) to be arranged in the test box (1) and corresponds to one end of each supporting rod (4) on the grid plates (2) to be connected, the other end of each connecting rod is arranged outside the test box (1) to be in sliding connection with the corresponding sliding block (502), more than one lug (5021) is arranged on the corresponding position of the arc-shaped sliding groove (101) of the test box (1), and more than one groove (102) matched with more than one lug (5021) is formed.
5. The potato root system in-situ observation test device according to claim 4, wherein the adjusting assembly (5) further comprises more than one telescopic rod (503), one end of the more than one telescopic rod (503) is respectively hinged with the grid plate (2), and the other end of the more than one telescopic rod is respectively hinged with the inner wall of the test box (1).
6. The potato root system in-situ observation test device according to claim 1, wherein the fixing mechanism (3) comprises two box covers (301) respectively hinged on the test box (1) and two clamping assemblies (302) respectively arranged on the two box covers (301), and the two clamping assemblies (302) can be spliced to clamp and fix potato plants.
7. The potato root system in-situ observation test device of claim 6, wherein the two clamping assemblies (302) are identical in structure and each comprise a limiting plate (3021), a clamping block (3022) slidably arranged on the limiting plate (3021), and a spring (3023) connecting the clamping block (3022) with the limiting plate (3021), the box cover (301) is provided with a notch (3011), and the limiting plate (3021) is arranged at the position of the box cover (301) corresponding to the notch (3011).
8. The potato root system in-situ observation test device according to claim 1, wherein the test box (1) is made of transparent material, grid lines (103) are arranged outside any two adjacent side surfaces of the test box (1), and the grid lines (103) are gradually dense from top to bottom.
9. The potato root system in-situ observation test device of claim 2, further comprising a nutrient solution tank (6), wherein a plurality of capillary holes are formed in the plurality of grid plates (2), a plurality of water outlet holes (201) communicated with the plurality of capillary holes are formed in the tops of the plurality of grid plates, and the plurality of grid plates (2) are communicated with the nutrient solution tank (6).
10. A potato test method, which adopts the potato root system in-situ observation test device as defined in any one of claims 1 to 9, and comprises the following operation steps:
s1, placing the potato tubers in a humid environment at the temperature of between 15 and 30 ℃ for 3 to 5 days continuously until the potato tubers sprout to form 8 to 10 cm plants;
s2, planting the germinated potato plants in a test box (1), fixing the leaves of the potatoes outside the test box (1) through a fixing mechanism (3), clamping the roots of the potatoes in the test box (1), placing the test box (1) in a cool environment, avoiding the potatoes from contacting with light, and moving the potatoes into an astigmatic environment after waiting for about one week;
s3, observing the growth condition of the roots of the potato plants in the test box (1) every 3 days, and shooting photos of the roots of the potato plants in the test box (1) by a camera for subsequent observation;
s4, observing the root conditions of the potato plants in the shooting test box (1) in different periods, observing the thickness and density of the roots and the number and size of the potato tubers, and making a table by statistical data.
CN202111355861.8A 2021-11-16 2021-11-16 Potato root system in-situ observation test device Pending CN114088892A (en)

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Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6088958A (en) * 1996-09-30 2000-07-18 Japan Tobacco, Inc. Process for producing potato tubers
CN2483948Y (en) * 2001-07-10 2002-04-03 华南农业大学 Vertical cultivation for plant root-system
WO2008156265A1 (en) * 2007-06-19 2008-12-24 Chang Ho So Culture dish and culture method of a potato trunk using the culture dish
CN104322248A (en) * 2014-10-09 2015-02-04 吉林省农业科学院 Layered positioning study method for plant root system growth and layered positioning device
CN105359870A (en) * 2015-12-03 2016-03-02 扬州大学 Apparatus for observing growth distribution of rice roots
CN205667202U (en) * 2016-06-06 2016-11-02 湖南农业大学 A kind of Real time visible device for observation of plant root growth
CN205671176U (en) * 2016-05-10 2016-11-09 侯坤 Double-deck point of root field planting plate
CN208523337U (en) * 2018-07-23 2019-02-22 沈阳农业大学 A kind of Root Growth of Rice configuration observation device
CN111937626A (en) * 2020-07-17 2020-11-17 武汉爱农云联科技有限公司 Potted plant maintenance device
CN212936948U (en) * 2020-06-29 2021-04-13 广西壮族自治区农业科学院 Barrel planting device easy for observing growth of sugarcane root system

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6088958A (en) * 1996-09-30 2000-07-18 Japan Tobacco, Inc. Process for producing potato tubers
CN2483948Y (en) * 2001-07-10 2002-04-03 华南农业大学 Vertical cultivation for plant root-system
WO2008156265A1 (en) * 2007-06-19 2008-12-24 Chang Ho So Culture dish and culture method of a potato trunk using the culture dish
CN104322248A (en) * 2014-10-09 2015-02-04 吉林省农业科学院 Layered positioning study method for plant root system growth and layered positioning device
CN105359870A (en) * 2015-12-03 2016-03-02 扬州大学 Apparatus for observing growth distribution of rice roots
CN205671176U (en) * 2016-05-10 2016-11-09 侯坤 Double-deck point of root field planting plate
CN205667202U (en) * 2016-06-06 2016-11-02 湖南农业大学 A kind of Real time visible device for observation of plant root growth
CN208523337U (en) * 2018-07-23 2019-02-22 沈阳农业大学 A kind of Root Growth of Rice configuration observation device
CN212936948U (en) * 2020-06-29 2021-04-13 广西壮族自治区农业科学院 Barrel planting device easy for observing growth of sugarcane root system
CN111937626A (en) * 2020-07-17 2020-11-17 武汉爱农云联科技有限公司 Potted plant maintenance device

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