WO2008156265A1

WO2008156265A1 - Culture dish and culture method of a potato trunk using the culture dish

Info

Publication number: WO2008156265A1
Application number: PCT/KR2008/003334
Authority: WO
Inventors: Chang Ho So; Ho Sam Ju; Soon Il So
Original assignee: Chang Ho So; Ho Sam Ju; Soon Il So
Priority date: 2007-06-19
Filing date: 2008-06-13
Publication date: 2008-12-24
Also published as: KR100863769B1

Abstract

A culture dish for increasing culturing efficiency and a culture method of a potato stem enabling mass and rapid production are disclosed. The culture dish includes a receptacle, a culture support plate, and a cover. The receptacle holds a culture medium on a bottom. The culture support plate is arranged horizontally in such a way as to be spaced apart from the bottom, and an upper surface of the culture support plate is partitioned by a plurality of partition walls which are placed side by side, thus forming a plurality of spaces, and the culture medium is injected to the upper surface of the culture support plate through a water supply hole. The cover functions to close the receptacle. Further, an air vent for ventilation is defined in one end of each of the spaces.

Description

[DESCRIPTION] [Invention Title]

CULTURE DISH AND CULTURE METHOD OF A POTATO TRUNK USING THE CULTURE DISH [Technical Field]

The present invention relates to a culture dish and a method of culturing a potato stem using the culture dish. [Background Art]

A culture dish is used to artificially control the germination of a seed or the growth of a plant stem, and a variety of plants may be so grown in the culture dish. Herein, the process of culturing a potato stem, which is performed during the production of a seed potato, will be described as one example.

Potatoes may be propagated using true seeds or propagules. However, when potatoes are cultivated in actual practice, the method of cultivating potatoes using propagules is the main method used.

Generally, in the case of the production of potatoes using propagules, virus-free and high-quality seed potatoes must be sown and cultivated so as to increase the harvest. However, the production and spread of virus-free and high-quality seed potatoes are substantially insufficient, so that the successful propagation rate of the seed potatoes in Korea is only 20 to 30%. In consideration of the proliferation system of seed potatoes, the mass production of potato seeds corresponding to a high grade quality of the basal species is difficult, and only a small production lot is possible. Therefore, the seed potatoes are proliferated for 4 to 6 years, and then are supplied to farmhouses.

Generally, in the first step of producing the seed potato, the growing point of a potato stem is cultured, so that the stem is formed. The stem is sub-cultured in the culture dish, and the cultured stem is cultured through a stem cutting method or a nutriculture method to produce a micro seed potato. Further, by changing the culture condition for the cultured stem, a microtuber is produced.

In the cases where the stem is cultured from the growing point of the potato stem or in the culture dish for creating a subculture, conventionally, as shown in FIG. 1, a culture medium is put into a culture dish 10, which has the shape of a container having a flat bottom. Thereafter, a plurality of stems P is placed on the culture medium so as to be cultured.

However, such a conventional culture method is problematic in that as the stems P are grown, the stems may become entangled with each other, and if two or more stems become entangled with each other, disentangling the stems P from each other must be done so as to sub-culture the stems or take potato seeds from the stems, thus making culturing complicated. Of course, the stems P may be easily damaged even by a small external force. Thus, during the operation of disentangling the entangled stems from each other, damage to the stems may occur, and so a long time is required for disentangling.

Further, the conventional culture method is problematic in that the stems which are placed horizontally on the culture dish 10 are not grown in a predetermined direction and may be curvy, thus making it inconvenient to check the seed potatoes and take the seed potatoes from the stems.

[Disclosure]

[Technical Problem]

Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a culture dish, which cultures a plurality of stems while keeping them separate from each other and keeping the growth of the stems uniform, and prevents the stems from becoming entangled with each other, thus allowing stem separation for sub-culturing the stems or taking seed potatoes from the stems to be easily done, therefore increasing culture efficiency, and providing a potato-stem culture method which enables mass and rapid production.

[Technical Solution]

In order to accomplish the above object, the present invention provides O

a culture dish, including a receptacle which holds a culture medium on a bottom; a culture support plate arranged horizontally in such a way as to be spaced apart from the bottom, an upper surface of the culture support plate being partitioned by a plurality of partition walls which are placed side by side, thus forming a plurality of spaces, the culture medium being injected to the upper surface of the culture support plate through a water supply hole! and a cover for closing the receptacle, wherein an air vent for ventilation is defined in one end of each of the spaces. [Advantageous Effects]

According to the present invention, an object to be cultured, that is a plurality of stems, is placed in separate spaces so as to prevent the stems from becoming entangled with each other and allow the stems to grow straight, thus making it easy to process and utilize the stems, and the stems are laid and arranged horizontally, so that water and nutriments are uniformly supplied from a culture medium to the stems, thus enabling the balanced growth of the stems. [Description of Drawings]

FIG. 1 is a perspective view illustrating a conventional culture dish for culturing stems;

FIG. 2 is an exploded perspective view illustrating a culture dish according to the first embodiment of the present invention;

FIG. 3 is an exploded perspective view illustrating a culture dish according to the second embodiment of the present invention;

FIG. 4 is a sectional view illustrating a culture dish according to a modification of the first and second embodiments of the present invention;

FIG. 5 is a view illustrating the state in which stems are cultured using the culture dish of FIG. 2 or 3; and

FIG. 6 is an exploded perspective view illustrating a culture dish according to the third embodiment of the present invention.

-Description of reference characters of important parts-

10, 100, 100' , 100" ; culture dish 110, 110' , 110" : receptacle

111: bottom 112: air vent

113, 113' : support frame 120, 120' : culture support plate

121: water supply hole 122: support leg

123, 123' , 131: partition wall

130, 130' : cover P: stem

[Mode for Invention]

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

FIG. 2 is an exploded perspective view illustrating a culture dish according to the first embodiment of the present invention, FIG. 3 is an exploded perspective view illustrating a culture dish according to the second embodiment of the present invention, and FIG. 4 is a sectional view illustrating a culture dish according to the modification of the first and second embodiments of the present invention.

The present invention relates to a culture dish 100 or 100' , and a method of culturing stems using the culture dish 100 or 100' . The culture dish 100 or 100' includes a receptacle 110 or 110' , a culture support plate 120, and a cover 130. The receptacle contains a culture medium, including water and nutriments which are required for the growth of the stems P. The culture support plate is held in the receptacle 110 or 110' , with the stems to be cultured being arranged on the culture support plate. The cover functions to close the open top of the receptacle 110 or 110' which holds the culture support plate 120 therein.

In the culture dish 100 or 100' according to the first or second embodiment of the present invention, the culture support plate 120 includes a plurality of partition walls 123 and 123' which are arranged side by side to partition the upper surface of the culture support plate 120 into a plurality of sections. Thus, when the receptacle is closed by the cover 130, the distinct spaces are formed by the cover and the partition walls 123 and 123 . Air ventilation through the partition walls 123 and 123' is blocked. Meanwhile, an air vent 112 is formed in one wall W of the receptacle 110 or 110' , so that ventilation is provided independently in the respective spaces partitioned by the partition walls 123 and 123' .

As described above, the air vent 112 serves to ventilate the spaces separated by the partition walls 123 and 123' . Thus, the ventilation of each space is provided only by an associated part of the air vent 112.

As shown in FIGS. 4a and 4b, even if the cover 130 covers to close off the top of the receptacle 110 or 110' , the air vent 112 or 112' of the receptacle 110 or 110' according to the first or second embodiment provides a gap for ventilation between the cover 130 and the receptacle 110 or 110' .

According to the first embodiment, as shown in FIGS. 2 and 4a, one wall W of the receptacle 110 is relatively lower than the opposite wall, so that a gap is defined between the lower surface of the cover 130 and the wall W. The spaces partitioned by the partition walls 123 and 123' are individually ventilated through this gap.

Meanwhile, according to the second embodiment, as shown in FIGS. 3 and 4b, the upper end of one wall W of the receptacle 110' is cut along the sections partitioned off by the partition walls 123 and 123' , thus forming a plurality of air vents 112' . Thereby, the sections are individually ventilated through the air vents 112' .

FIG. 4c illustrates air vents according to the modification of the present invention. The air vents 112' are formed in one wall W of the receptacle 110' , and in addition, air vents 132 are formed in the cover 130 to correspond to the air vents 112' . Thereby, the air vents 112' and 132 communicate with each other, thus ventilating the interior of the receptacle 110' .

The air vent of the culture dish 100 or 100' according to the present invention is not limited to the above-mentioned embodiments.

That is, the air vent 112 or 112' according to the present invention may comprise one or more holes which are bored through one wall W (see FIG. 4c). Further, instead of the receptacle, a hole may be formed in a planar portion of the cover to serve as the air vent. Further, the cover may be formed to be shorter than the receptacle so that the cover does not cover the entire portion of the receptacle, and part of the receptacle is left open, thereby allowing respective spaces to be ventilated through the open part.

The air vent 112 or 112' may be formed using various methods. However, the air vent 112 or 112' must be formed in only one end of each of the sections separated off by the partition walls 123 or 123' . Consequently, the ventilation in the spaces occurs mostly only in one location.

Water supply holes 121 are cut in the culture support plate, and are used to supply the water and nutriments of the culture medium, injected into a bottom 111 in the receptacle 110 or 110' , to the stems P arranged on the upper surface of the culture support plate 120. The stems P are placed at ends of the small spaces, which are opposite the air vent 112, and grow towards the air vent 112. A plurality of grown stems P is arranged in the lengthwise direction such that water and nutriments can be supplied to any portion of each of the small spaces separated by the partition walls 123 and 123' (see FIG. 5).

The culture support plate 120 is spaced apart from the bottom 111, thus ensuring a space for holding the culture medium in the culture dish. Thus, the culture dish includes a plurality of support legs 122 which supports a main body of the culture support plate 120. Of course, the culture support plate 120 may be supported by means other than the support legs 122. The culture dish may be modified, as in the third embodiment.

The cover 130 covers the top of the receptacle 110 or 110' to close it. Preferably, the cover is firmly secured to the receptacle 110 or 110' such that the cover is not removed or become separated from the receptacle.

Meanwhile, the cover 130 is made of a transparent or a semitransparent material permitting the transmission of light. However, the cover may be made of an opaque material which prevents the transmission thereof.

Hereinafter, the culture method using the culture dish 100 according to the first or second embodiment of the present invention will be described sequentially. Herein, an object to be cultured is a potato stem for the production of a seed potato. But, the potato stem, which is the object to be cultured, is only an example for illustrating the culture dish 100 or 100' and the culture method according to the present invention. It is apparent that the culture dish 100 or 100' is not only for a potato stem.

First step: Yielding a basal stem

The growing point of a potato is taken and cultured, so that a basal stem is yielded. Here, the growing point is taken from an apical meristem of a bud which is germinated by the cultured potato.

The taken growing point is laid on the culture support plate 120. Here, the growing point will be located at a point which is opposite the air vent 112 in each of the small spaces separated off by the partition walls 123 or 123' .

Meanwhile, an MS medium, this being generally used to culture plant tissue, is utilized as the culture medium for culturing the growing point, and is injected into the receptacle 110 of the culture dish 100 or 100' according to the present invention.

The culture medium contains potassium nitrate, ammonium nitrate, potassium phosphate, calcium chloride, and other substances and has a pH from 5.5 to 6.0. The culturing conditions require a temperature range of from 20 to 30^°C , illuminance of 2,000 lux or more, and an illuminating period whose light period and dark period have a ratio from 13:11 to 17:7 (in hours).

The stem P is proliferated from the growing point, and grows along each small space towards the air vent 112. This happens because oxygen and carbon dioxide, indispensable for the growth of the stem P, are supplied only through the air vent 112. Further, neighboring small spaces are separated from each other by the partition walls 123 and 123' , thus preventing growing stems P from becoming entangled with each other, and allowing the stems P to grow straight .

Moreover, each stem P is arranged horizontally, so that water and nutriments are directly and uniformly supplied through the plurality of water supply holes 121 to the stem. Thus, the entire portion of the stem P is evenly grown to have good nutritive conditions, and is uniformly proliferated.

If the stem P grows in length to 3 to 7cm, the proliferated stem is sub-cultured, so that the basal stem is yielded.

Second step: Proliferation of the basal stem

The basal stem, yielded through the first stage, is sub-cultured in another culture dish 100 or 100' , so that a proliferated stem is yielded.

Preferably, the basal stem P yielded at the first step is cultured using a liquid medium rather than a solid medium, in consideration of the growth rate of the stem P. However, when a liquid medium is chosen, conventionally, the stem may be immersed into the medium, and the uniform growth of stems may be impeded due to the interference between the stems growing in the medium during the culturing period. For the reason, until now, the stems have been cultured and proliferated in the solid medium. However, according to the present invention, the basal stem P is grown in each of the small spaces partitioned by the partition walls 123 and 123' , thus overcoming the disadvantage of the liquid medium, while utilizing the advantage of the liquid medium which is that it is capable of increasing the growth rate, therefore maximizing culture efficiency.

Meanwhile, the culture condition when the basal stem P is cultured using the culture dish 100 or 100' is identical with that of the first step.

Third step: Production of seed potato

The stem, proliferated at the second step, is cultured in a culture medium for forming a seed potato at 15 to 25°C under a condition of darkness, and thus the seed potato is produced.

Preferably, the stem, grown to 5 to 15cm, is cut to a proper length, and then the cut stem is used. The culture medium for forming the seed potato contains a triazole-based compound, such as uniconazole, an anti- gibberellin compound, paclobutrazol , inabenfide, ancymidol , or flurprimidol . Alternatively, the culture medium may use an MS medium as the basic substance and partially contain the triazole-based compound. It is preferable that the stem be cultured under the following conditions, that is, for 50 to 60 days at a temperature of from 15 to 25^°C .

Of course, even in this case, the culture dish 100 or 100' according to the present invention can be utilized. Consequently, the entire lengthwise portion of the final stem for producing the seed potato exhibits uniform growth, and has a straight shape, so that it is easy to take the seed potato from the stem. Further, the stems are not entangled with each other, thus completely obviating damage to the stems resulting from their needing to be separated.

FIG. 6 is an exploded perspective view illustrating a culture dish according to the third embodiment of the present invention. The culture dish will be described with reference to this drawing.

The culture dish 100" according to the third embodiment is constructed as follows.

A support frame 113 protrudes from the inner wall of a receptacle 110". The support frame 113 functions to support a culture support plate 120' , in place of the support legs 122 of the first and second embodiments. Since the support frame 113 is spaced apart from a bottom 111 by a predetermined distance, the culture support plate 120' , held on the support frame 113, is spaced apart from the bottom 111.

Meanwhile, the culture support plate 120' has a mesh shape, and functions to evenly supply the water and nutriments of a culture medium, injected to the bottom 111, to the entire portion of the culture support plate 120' .

A plurality of partition walls 131 is provided on the lower surface of a cover 130' . The partition walls 131, provided on the lower surface of the cover 130' , have the same function as the partition walls 123 and 123' of the culture support plate 120 according to the first or second embodiment, and are used instead of the partition walls 123 and 123' of the culture support plate 120. The plurality of partition walls is arranged side by side in such a way as to be spaced apart from each other, thus partitioning the upper surface of the mesh-shaped culture support plate 120' into separate small spaces.

The third embodiment 100" modifies the culture dish 100 or 100' according to the present invention, and the partition walls 123 and 123' may be provided on the mesh-shaped culture support plate 120' , as in the first and second embodiments.

Claims

[CLAIMS] [Claim 1]

A culture dish, comprising: a receptacle (110) having a shape of a container enclosed by a sidewall to hold a culture medium on a bottom; a culture support plate (120) arranged horizontally in such a way as to be spaced apart from the bottom, an upper surface of the culture support plate being partitioned by a plurality of partition walls (123) which are placed side by side, thus forming a plurality of spaces, the culture medium being injected to the upper surface of the culture support plate through a water supply hole (121); and a cover (130) for closing the receptacle (110), wherein an air vent (112) for ventilation is defined in one end of each of the spaces. [Claim 2]

The culture dish according to claim 1, wherein the culture support plate (120) has a lattice shape, with the water supply hole (121) formed in the culture support plate. [Claim 3]

The culture dish according to claim 1, wherein the air vent (112) comprises air vents which are cut in one wall (W) of the receptacle (110) so that the air vents are individually formed in the corresponding spaces portioned off by the partition walls (123). [Claim 4]

A culture dish, comprising: a receptacle having a shape of a container enclosed by a sidewall to hold a culture medium on a bottom; a culture support plate (120' ) arranged horizontally in such a way as to be spaced apart from the bottom, the culture medium being injected to an upper surface of the culture support plate through a water supply hole (121); and a cover (130' ) for closing the receptacle, and having on a lower surface thereof a plurality of partition walls (131) which are arranged side by side, the partition walls placed on the upper surface of the culture support plate when the cover closes the receptacle, thus partitioning the upper surface of the culture support plate into a plurality of spaces, wherein an air vent for ventilation is defined in one end of each of the spaces. [Claim 5]

The culture dish according to claim 4, wherein the culture support plate (120' ) has a lattice shape, with the water supply hole formed in the culture support plate. [Claim 6]

A method of culturing a potato stem, comprising: a first step of culturing a growing point taken from a potato, thus yielding a basal stem; a second step of sub-culturing and proliferating the basal stem; and a third step of culturing the proliferated stem on a culture medium for forming a seed potato, wherein any one step selected from the steps is performed in a culture dish, the culture dish comprising: a receptacle (110) having a shape of a container enclosed by a sidewall to hold a culture medium on a bottom; a culture support plate (120) arranged horizontally in such a way as to be spaced apart from the bottom, an upper surface of the culture support plate being partitioned by a plurality of partition walls (123) which are placed side by side, thus forming a plurality of spaces, the culture medium being injected to the upper surface of the culture support plate through a water supply hole (121); and a cover (130) for closing the receptacle (110), wherein an air vent (112) for ventilation is defined in one end of each of the spaces, wherein the growing point of the first step, the basal stem of the second step, or the proliferated stem of the third step is arranged at an end opposite the air vent and is cultured. [Claim 7]

A method of culturing a potato stem, comprising: a first step of culturing a growing point taken from a potato, thus yielding a basal stem; a second step of sub-culturing and proliferating the basal stem; and a third step of culturing the proliferated stem on a culture medium for forming a seed potato, wherein any one step selected from the steps is performed in a culture dish, the culture dish comprising: a receptacle having a shape of a container enclosed by a sidewall to hold a culture medium on a bottom; a culture support plate (120' ) arranged horizontally in such a way as to be spaced apart from the bottom, the culture medium being injected to an upper surface of the culture support plate through a water supply hole (121); and a cover (130' ) for closing the receptacle, and having on a lower surface thereof a plurality of partition walls (131) which are arranged side by side, the partition walls placed on the upper surface of the culture support plate when the cover closes the receptacle, thus partitioning the upper surface of the support plate into a plurality of spaces, wherein an air vent for ventilation is defined in one end of each of the spaces, wherein the growing point of the first step, the basal stem of the second step, or the proliferated stem of the third step is arranged at an end opposite the air vent and is cultured.