JPS63287478A - Box for cultivating biological tissue - Google Patents

Abstract

PURPOSE:To obtain a cultivation box for biological tissues capable of efficiently carrying out transplanting, by dividing the interior or a transparent receptacle unit in the box consisting of the receptacle unit and a lid unit with partition frames and filling a culture medium supporting material in the bottom of spaces in the respective frames. CONSTITUTION:A cultivation box for biological tissues is constituted of a transparent receptacle unit (1a), colorless and transparent lid unit (1b), partition frames 5 for dividing the interior of the receptacle unit (1a) and a culture medium supporting material 6 to be filled in the bottom of spaces (5a) in the respective frames. Agar, rock wool, water absorbing resin foam, polyester fiber, cellulose, etc., are used alone or suitably mixed for use as the culture medium supporting material 6.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a biological & Il fabric culture box suitably used for culturing biological & Il fabrics, particularly plant tissues.

[Prior art and its problems]

Recently, research on plant Mi-woven dark culture has been actively conducted, and the use of plant tissue culture bacteria has become popular for some high-grade garden plants.

Such plant & 11 tissue culture uses -C a culture container made of glass, differentiates explants that have been hopelessly excised from the plant body in this culture container, and multiplies the regenerated plantlets. This is done by growing seedlings into cultured seedlings, which are removed from the culture container and acclimatized.

In this method, if contamination occurs due to bacteria entering the culture container during culture, all the callus and plantlets inside the culture container will be contaminated. The culture is carried out in small quantities using containers, etc., in order to minimize the damage caused by contamination.

However, if the culture container is small as mentioned above,
It is not possible to culture in large quantities, and it is troublesome to remove cultured seedlings from the small opening of the culture container for acclimatization and transplant them, and there are problems such as easily damaging the roots of cultured seedlings when removing them. Ta.

[Means for solving problems]

The biological tissue culture box of the present invention was completed as a result of intensive research with the aim of solving the above problems all at once. The gist is that the inside of the saucer body is divided by isolation frames, and the lower part of each space within each isolation frame is filled with a culture medium support material.

〔Example〕

Embodiments of the present invention will be described in detail below with reference to the drawings.

FIG. 1 is an exploded perspective view showing a biological M tissue culture box according to an embodiment of the present invention with its lid partially cut away, and FIG. 2 is a sectional view of the box.

In the figure, la is a rectangular saucer body, and 1b is the saucer body 1a.
A substantially cubic box 1 is constituted by the saucer body 1a and the lid body 1b. Both the saucer body 1a and the lid body 1b are made of colorless and transparent synthetic resin such as heat-resistant polycarbonate or polyester sarcon.
Alternatively, the lid body ib is formed on the four walls of the saucer body 1a, and the raised lid holder is fitted into the stepped portion 11a, and a pair of stainless steel plates with good corrosion resistance are formed. It is fixed by a stop 3.3. And this lid body 1b
As shown in an enlarged view in FIG. 3, a packing material 4 is interposed between the lid holder step portion 11a and the lid holder step portion 11a to prevent the invasion of germs. Further, a plurality of ventilation holes 11b are bored in the top plate portion of the lid body 1b, and cotton plugs or the like can be stuffed into the ventilation holes 11b. There are no particular restrictions on the vertical, horizontal, and height dimensions of the entire box l, but considering the convenience of transportation and handling, approximately 20
0X2QOX200m+a to 300X300X300
It is preferable to set the dimension range to about +*-.

A lattice-shaped isolation frame 5 is built into the interior of the saucer body 1a, and the interior of the saucer body 1a is finely divided by the isolation frame 5. The isolation frame 5 is made of the same synthetic resin as the saucer body 1a, the lid body 1b, etc., and as shown in FIG. It has become. There are no particular restrictions on the number and volume of small compartments divided by this isolation frame 5, in other words, the number and volume of the spaces 5a within the frame, but there are considerations such as the workability of transplantation or the quality of differentiation, proliferation, and growth. Considering, the space within the frame 5a
It is preferable to set the number of frames to about 100 to 200, the vertical and horizontal dimensions of the frame space 5a to about 10 to 20++a+, and the volume of the frame space 5a to about 1.5 to 5.9 cc.

A culture medium support material 6 is provided at the bottom of each frame space 5a of the isolation frame 5.
is filled, and an explant (not shown) that is aseptically excised from the plant body is transplanted therein. Such culture medium support material 6 is at least one part from the upper end of each frame space 5a.
It is preferable to fill the medium so that about 5-10 degrees of space remains as an empty space.If the medium is filled so that a sufficient space is left at the top in this way, even if the culture medium support material 6 in one frame space 5a is contaminated with bacteria. Even so, it becomes difficult for germs to move to the culture medium support material 6 in the adjacent frame space, which has the advantage of almost certainly preventing the spread of contamination.

As the medium support material 6, for example, in addition to agar, rock wool, water-absorbing resin foam, polyester fiber, cellulose, etc. may be used alone or in an appropriate mixture.

In the biological tissue culture box of this embodiment, as described above, the lid holder of the tray body 1a is configured to be sealed by interposing the packing material 4 between the stepped portion 11a and the lid body 1b. Alternatively, a sealing structure as shown in FIG. 4 may be adopted. In other words, in FIG.
ie and outer wall lld are formed without a bifurcated shape A, and a saucer body l is inserted between these inner and outer walls 11c and lid via a packing material 4.
The upper edge of a is fitted and sealed, and this structure has the advantage of further improving sealing performance and more reliably preventing invasion of germs. Further, in the box of this embodiment, as described above, the ventilation hole 11b is formed in the top plate of the lid body 1 and is configured to be filled with a cotton plug or the like, but instead of this, as shown in FIG. Of course, it is also possible to adopt a ventilation structure such as that shown in FIG. With this structure, the box 1 can be changed from a ventilated state to an airtight state by removing the camp 8 and replacing the sterile filter 8 with a closing plate. It has the advantage of being easily changeable. It goes without saying that the ventilation structure as shown in FIG. 5 and the aforementioned ventilation holes 11b may be formed on the side wall of the lid body 1b as required.

When performing tissue culture using the biological tissue culture box of the present invention configured as described above, first, the lid/body 1b is removed, and the culture medium support material 6 filled in each frame space 5a of the isolation frame 5 is removed. After transplanting the explant, cover the lid body 1b and fix it with the fastener 3. Then, the explant is heterotrophically differentiated for several months inside the box 1, and a plantlet is regenerated. During this initial culture period, contamination is generally likely to occur due to the invasion of various bacteria, but in the biological fabric culture box of the present invention, as described above, the lid holder of the tray body 1a is located between the stepped portion 11a and the lid body 1b. Since the box 1 is sealed with a gasket 4 interposed between the box 1 and the inside of the box 1, there is little intrusion of germs into the box 1 (almost no contamination occurs). Since the lower part of each frame space 5a is filled with a culture medium support material 6 and the upper part of each frame space 5a is left empty, even if the culture medium support material 6 in one frame space 5a is contaminated with bacteria. Even if there is a possibility that the bacteria may move beyond the isolation frame 5 to the culture medium support material 6 in the adjacent frame space, it is possible to almost certainly prevent the spread of contamination. Therefore, damage caused by contamination can be minimized.

The regenerated plantlets continue to grow in the box 1, and when they become cultured bacteria, the whole box 1 is transferred to the acclimation process. Then, in the middle of acclimatization, remove the lid body 1b and remove the saucer body l.
Transfer the whole plant to a greenhouse, etc., allow it to acclimate in earnest, and then transplant it to a pallet containing artificial soil, etc., to use as a permanent seedling.

In this way, in the biological tissue culture box of the present invention, the cultured bacteria can be transferred to the acclimatization process along with the box 1.
There is no longer any need for the troublesome work of taking out cultured bacteria one by one from a culture container and transplanting them as in the past, and there is no need to damage the cultured bacteria. In addition, when transferring cultured bacteria to the acclimatization process,
Cultured bacteria may be taken out and transplanted one by one in the same way as before, but even in that case, since the cultured bacteria is separated by the isolation frame 5, the cultured bacteria cannot be removed as in the case of taking out the cultured bacteria from a conventional small culture container. There is almost no fear of damage, and since the lid TB can be removed and the cultured bacteria can be taken out in a free position, there is no need to insert a jig into the small opening of a conventional small culture container to take out the cultured bacteria. This has the advantage that the workability of removing cultured bacteria is greatly improved.

〔Effect of the invention〕

As is clear from the above explanation, according to the biological tissue culture box of the present invention, it is possible to almost certainly prevent contamination, and even if the culture medium support material is contaminated with bacteria in one of the isolation frames, Even if the contamination occurs, the spread of contamination to the surrounding area can be prevented and the damage can be minimized, and there is no need to worry about damaging the cultured bacteria since the troublesome work of transplanting the cultured bacteria is not necessary. Without,
Even in the case of transplantation, remarkable effects such as the ability to remove the lid body and carry out the transplantation work efficiently can be obtained.

[Brief explanation of drawings]

FIG. 1 is an exploded perspective view showing a biological tissue culture box according to an embodiment of the present invention with the lid partially cut away, and FIG. 2 is a sectional view of the box with the lid attached. Fig. 3 is an enlarged partial sectional view of the fitting portion between the saucer body and the lid body, Fig. 4 is an enlarged partial sectional view showing different fitting structures of the saucer body and the lid body, and Fig. 5 is a lid with a different ventilation structure. FIG. 2 is an enlarged partial cross-sectional view of the body. (Explanation of main symbols in the drawings) l...box, 1a...saucer body, lb...lid body, 5...isolation frame, 5a...frame space, 6...culture medium support material . Patent applicant Yasushi Watanabe Co., Ltd. C)

Claims (1)

[Claims] (1) A box is constructed of a transparent saucer body and a lid body, the inside of this saucer body is divided by isolation frames, and the lower part of each frame space of the isolation frame is filled with a culture medium support material. Tissue culture box.