CN114081856A - PDRN-containing essence with whitening, spot-fading, moisturizing and skin-tendering functions and preparation process thereof - Google Patents

Abstract

The invention discloses PDRN-containing essence with functions of whitening, fading spots, moisturizing and tendering skin and a preparation process thereof, wherein the essence comprises the following raw materials in parts by weight: PDRN1-10 parts, saussurea involucrate extracting solution 5-10 parts, centella asiatica extracting solution 3-8 parts, nicotinamide 3-12 parts, hyaluronic acid 3-6 parts, glycerol 5-10 parts, hydroxyethyl cellulose 2-4 parts, polyacrylic acid 0.5-1 part, p-hydroxyacetophenone 0.3-0.6 part, hydrogenated castor oil 0.2-0.5 part, citric acid 0.05-0.1 part, and deionized water is used for supplementing to 100 parts. The cosmetic prepared by the invention has the effects of moisturizing, whitening, lightening spots and improving microcirculation. The PDRN in the invention is micromolecular DNA extracted from spermary of salmon, has the size of between 100 and 500bp, can be transferred to the dermis layer of skin, replenishes water and moistens, repairs the skin and promotes whitening regeneration. The skin whitening and spot whitening moisturizing cream is mild and non-irritant to skin, has no greasy feeling, has the effects of whitening, spot lightening, moisturizing and tendering skin, and can be used for a long time.

Description

PDRN-containing essence with whitening, spot-fading, moisturizing and skin-tendering functions and preparation process thereof

Technical Field

The invention belongs to the field of biotechnology and cosmetics, and relates to whitening, spot-lightening, moisturizing, skin-tendering and anti-aging emulsion containing PDRN (Polygala tenuifolia Willd.) and saussurea involucrate extracts and a preparation method thereof.

Background

The human face can visually reflect the mental appearance and the physical health of a person, and the quality and the aesthetic feeling of the person are shown. With the continuous improvement of the living standard of human beings, people have increasingly strong pursuit of beauty. The market demand of cosmetics for whitening, spot lightening, skin tendering and anti-aging is continuously increasing. Research shows that seventy percent of European and American women use the cosmetics with the functions of whitening and removing freckles to relieve and clear spots and chloasma; eighty percent of asian women desire to have fair and shiny skin on their own. Most whitening cosmetics on the market at present have poor freckle removing effect, are added with essence and hormone, have side effects and are easy to generate dependency, so that the whitening and freckle removing cosmetic has wide prospect for developing efficient and nontoxic whitening and freckle removing products.

Polydeoxyribonucleotides (PDRNs) are natural low-molecular-weight DNA molecules with the length of 50-2000 bp, and the natural sources of the PDRNs are mainly human placenta or trout sperms. PDRNs bind to water molecules to form a 3D gel and are cleaved into nucleotides, which naturally occur in the ECM and are used by cells for metabolism. In addition to being involved in cell growth, PDRNs promote the production of Vascular Endothelial Growth Factor (VEGF) and collagen, the migration of various cells, and reduce inflammatory responses. Both increased VEGF production and decreased inflammation are associated with promoting tissue healing. The major mechanism by which PDRNs are known to function is their interaction with adenosine receptors, particularly the A2A receptor. Based on its multiple effects, it has been used to improve the skin repair process, enhance the wound healing strength, tissue repair, wound regeneration and skin beauty of diabetic people.

Disclosure of Invention

Technical problem to be solved

The invention aims to provide a safe and effective essence containing PDRN active ingredients and having the functions of whitening, lightening spots, moisturizing and tendering skin and a preparation method thereof. The technology described in the invention has the advantages of simple and convenient operation, safe and nontoxic formula, long-term use and obvious effects of whitening, lightening spots, moisturizing, tendering skin and resisting aging.

The technical scheme provided by the invention is as follows:

the essence containing PDRN and having the functions of whitening, fading spots, supplementing water and preserving moisture is prepared from the following raw materials, by weight, 1-10 parts of PDRN, 5-10 parts of saussurea involucrate extract, 3-8 parts of centella asiatica extract, 3-12 parts of nicotinamide, 3-6 parts of hyaluronic acid, 5-10 parts of glycerol, 2-4 parts of hydroxyethyl cellulose, 0.5-1 part of polyacrylic acid, 0.3-0.6 part of p-hydroxyacetophenone, 0.2-0.5 part of hydrogenated castor oil and 0.05-0.1 part of citric acid, and deionized water is used for supplementing to 100 parts.

Further, the feed is prepared from the following raw materials in parts by weight: 2 parts of PDRN, 5 parts of saussurea involucrate extract, 6 parts of centella asiatica extract, 5 parts of nicotinamide, 4 parts of hyaluronic acid, 6 parts of glycerol, 3 parts of hydroxyethyl cellulose, 0.5 part of polyacrylic acid, 0.4 part of p-hydroxyacetophenone, 0.3 part of hydrogenated castor oil and 0.05 part of citric acid, and the 100 parts of deionized water is used for supplementing.

Further, the feed is prepared from the following raw materials in parts by weight: 5 parts of PDRN, 4 parts of saussurea involucrate extract, 5 parts of centella asiatica extract, 5 parts of nicotinamide, 5 parts of hyaluronic acid, 6 parts of glycerol, 3 parts of hydroxyethyl cellulose, 0.5 part of polyacrylic acid, 0.4 part of p-hydroxyacetophenone, 0.3 part of hydrogenated castor oil and 0.05 part of citric acid, and the 100 parts of deionized water is used for supplementing.

Further, the PDRN extraction step comprises: taking out the spermary of the salmon fish, cleaning the spermary with precooled normal saline, placing the cleaned spermary in an extraction buffer solution, and homogenizing and crushing by using a homogenizing crusher; adding proper protease K to enzymolyze protein; thirdly, centrifuging, collecting supernatant, adding saturated sodium chloride solution into the supernatant, mixing uniformly, centrifuging and collecting supernatant containing DNA; adding absolute ethyl alcohol into the collected supernatant, precipitating DNA, and washing the precipitate twice with 75% ethyl alcohol; dissolving the obtained DNA molecules by using deionized water, and carrying out ultrasonic crushing; sixthly, freeze drying to obtain the micromolecule Polydeoxyribonucleotide (PDRN).

Further, the preparation method of the snow lotus extract and the snow grass extract comprises the following steps:

respectively cleaning herba Adonidis and herba Centellae, oven drying, and pulverizing; extracting the crushed powder in a supercritical extraction reactor; the extractant used was ethanol: butanediol (3: 3-8), the temperature is 45-75 ℃, the pressure is 30-40MPa, and the circular extraction is carried out for 3-4 hours; filtering the extractive solution, and concentrating to obtain herba Saussureae Involueratae extractive solution and herba Centellae extractive solution.

Further, the method comprises the following steps:

1) extracting small molecule Polydeoxyribonucleotide (PDRN) from salmon according to the extraction step of PDRN;

2) the preparation method comprises the following steps of weighing the snow lotus herb extract, the centella asiatica extract, the nicotinamide, the hyaluronic acid, the glycerol, the hydroxyethyl cellulose, the polyacrylic acid, the p-hydroxyacetophenone and the hydrogenated castor oil according to the weight part of the raw materials in claim 1, slowly adding the components into deionized water in sequence, stirring for 1-3 hours at the temperature of 60-72 ℃ by using a stirrer, adding the citric acid in corresponding parts by mass, continuously stirring uniformly, standing for 1 hour to be stable, adding the PDRN, fully stirring uniformly, and standing to room temperature to obtain the essence.

The beneficial effects are that:

PDRN is a small molecule DNA from salmon sperm cells, and has extremely high sequence similarity with human DNA. It can act on adenosine A2a receptor, thereby promoting VEGF production, promoting cell regeneration, improving skin condition, and improving skin color. In addition, a number of studies have shown that PDRN is free of systemic toxicity and antigenicity and is therefore a safe cosmetic ingredient.

2. The herba Saussureae Involueratae has effects of whitening skin, resisting dampness, preventing wrinkle, resisting aging, and promoting skin cell regeneration.

3. The nicotinamide can relieve and prevent skin dark yellow color generated in the early aging process of the skin, can repair damaged stratum corneum lipid barrier of the skin, improves the skin resistance, and has extremely strong water locking function. The research shows that the nicotinamide can effectively inhibit the diffusion of melanin to the horny layer, and can accelerate the cell renewal speed and promote the melanocyte to fall off when part of the melanin inevitably reaches the surface layer of the skin. Thus having good whitening effect.

4. The snow lotus herb extract and the snow grass extract in the invention can reduce the irritation of nicotinamide to skin and simultaneously play a role in whitening by the cooperation of various substances.

5. Hyaluronic acid has the effects of moisturizing, delaying aging, increasing skin elasticity and the like, and is a common component of cosmetics. Research shows that when PDRN and hyaluronic acid are used together, the effect of promoting wound healing is obviously higher than that of hyaluronic acid used alone. The efficacy of the two in promoting each other is shown.

Detailed Description

The essence containing PDRN and having the functions of whitening, fading spots, supplementing water and preserving moisture is prepared from the following raw materials, by weight, 1-10 parts of PDRN, 5-10 parts of saussurea involucrate extract, 3-8 parts of centella asiatica extract, 3-12 parts of nicotinamide, 3-6 parts of hyaluronic acid, 5-10 parts of glycerol, 2-4 parts of hydroxyethyl cellulose, 0.5-1 part of polyacrylic acid, 0.3-0.6 part of p-hydroxyacetophenone, 0.2-0.5 part of hydrogenated castor oil and 0.05-0.1 part of citric acid, and deionized water is used for supplementing to 100 parts.

Further, the feed is prepared from the following raw materials in parts by weight: 2 parts of PDRN, 5 parts of saussurea involucrate extract, 6 parts of centella asiatica extract, 5 parts of nicotinamide, 4 parts of hyaluronic acid, 6 parts of glycerol, 3 parts of hydroxyethyl cellulose, 0.5 part of polyacrylic acid, 0.4 part of p-hydroxyacetophenone, 0.3 part of hydrogenated castor oil and 0.05 part of citric acid, and the 100 parts of deionized water is used for supplementing.

Further, the feed is prepared from the following raw materials in parts by weight: 5 parts of PDRN, 4 parts of saussurea involucrate extract, 5 parts of centella asiatica extract, 5 parts of nicotinamide, 5 parts of hyaluronic acid, 6 parts of glycerol, 3 parts of hydroxyethyl cellulose, 0.5 part of polyacrylic acid, 0.4 part of p-hydroxyacetophenone, 0.3 part of hydrogenated castor oil and 0.05 part of citric acid, and the 100 parts of deionized water is used for supplementing.

Further, the PDRN extraction step comprises: taking out the spermary of the salmon fish, cleaning the spermary with precooled normal saline, placing the cleaned spermary in an extraction buffer solution, and homogenizing and crushing by using a homogenizing crusher; adding proper protease K to enzymolyze protein; thirdly, centrifuging, collecting supernatant, adding saturated sodium chloride solution into the supernatant, mixing uniformly, centrifuging and collecting supernatant containing DNA; adding absolute ethyl alcohol into the collected supernatant, precipitating DNA, and washing the precipitate twice with 75% ethyl alcohol; dissolving the obtained DNA molecules by using deionized water, and carrying out ultrasonic crushing; sixthly, freeze drying to obtain the micromolecule Polydeoxyribonucleotide (PDRN).

Further, the preparation method of the snow lotus extract and the snow grass extract comprises the following steps:

respectively cleaning herba Adonidis and herba Centellae, oven drying, and pulverizing; extracting the crushed powder in a supercritical extraction reactor; the extractant used was ethanol: butanediol (3: 3-8), the temperature is 45-75 ℃, the pressure is 30-40MPa, and the circular extraction is carried out for 3-4 hours; filtering the extractive solution, and concentrating to obtain herba Saussureae Involueratae extractive solution and herba Centellae extractive solution.

Further, the method comprises the following steps:

1) extracting small molecule Polydeoxyribonucleotide (PDRN) from salmon according to the extraction step of PDRN;

2) the preparation method comprises the following steps of weighing the snow lotus herb extract, the centella asiatica extract, the nicotinamide, the hyaluronic acid, the glycerol, the hydroxyethyl cellulose, the polyacrylic acid, the p-hydroxyacetophenone and the hydrogenated castor oil according to the weight part of the raw materials in claim 1, slowly adding the components into deionized water in sequence, stirring for 1-3 hours at the temperature of 60-72 ℃ by using a stirrer, adding the citric acid in corresponding parts by mass, continuously stirring uniformly, standing for 1 hour to be stable, adding the PDRN, fully stirring uniformly, and standing to room temperature to obtain the essence.

Embodiment mode 1

The PDRN-containing essence with the functions of whitening, fading spots, moisturizing and tendering skin is prepared from the following raw materials in parts by weight: 2 parts of PDRN, 5 parts of saussurea involucrate extract, 6 parts of centella asiatica extract, 5 parts of nicotinamide, 4 parts of hyaluronic acid, 6 parts of glycerol, 3 parts of hydroxyethyl cellulose, 0.5 part of polyacrylic acid, 0.4 part of p-hydroxyacetophenone, 0.3 part of hydrogenated castor oil and 0.05 part of citric acid, and the 100 parts of deionized water is used for supplementing.

The preparation method comprises the following specific steps:

taking out the salmon testis, cleaning with pre-cooled physiological saline, adding into extraction buffer solution (containing Tris-HCl, NaCl, Na2EDTA), homogenizing, and crushing; adding a proper amount of protease K to carry out enzymolysis on protein; placing the mixture into a centrifugal machine, centrifuging at the rotating speed of 10000rpm, and collecting supernatant; adding an equal volume of saturated sodium chloride solution into the supernatant, uniformly mixing, and centrifuging to collect the supernatant containing DNA; adding equal volume of absolute ethyl alcohol into the collected supernatant, precipitating DNA at low temperature, and washing twice with 75% ethyl alcohol; dissolving precipitate (DNA) in deionized water, and breaking the DNA by an ultrasonic crusher; freeze drying to obtain small molecule Polydeoxyribonucleotide (PDRN).

Drying the killed snow lotus herb and centella asiatica, crushing, sieving by a 40-mesh sieve, and circularly extracting for 3 hours at 55 ℃ under the protection of carbon dioxide by using an extracting agent (ethanol: butanediol: 3: 5). Filtering the extractive solution, distilling under reduced pressure, and concentrating for 2 hr to obtain herba Adonidis extract and herba Centellae extract.

Weighing 5 parts of snow lotus extract, 6 parts of snow grass extract, 5 parts of nicotinamide, 4 parts of hyaluronic acid, 6 parts of glycerol, 3 parts of hydroxyethyl cellulose, 0.5 part of polyacrylic acid, 0.4 part of p-hydroxyacetophenone and 0.3 part of hydrogenated castor oil, slowly and sequentially adding the components into deionized water, stirring for 2 hours at 65 ℃ by using a stirrer, adding 0.05 part of citric acid, continuously stirring to be uniform, standing for 1 hour to be stable, adding 2 parts of PDRN, fully stirring to be uniform, and standing to room temperature to obtain the whitening, spot-lightening, moisturizing and skin-tendering essence containing PDRN.

Embodiment mode 2

A whitening, spot-fading, moisturizing and skin-tendering essence containing PDRN is prepared from the following raw materials in parts by weight: 5 parts of PDRN, 4 parts of saussurea involucrate extract, 5 parts of centella asiatica extract, 5 parts of nicotinamide, 5 parts of hyaluronic acid, 6 parts of glycerol, 3 parts of hydroxyethyl cellulose, 0.5 part of polyacrylic acid, 0.4 part of p-hydroxyacetophenone, 0.3 part of hydrogenated castor oil and 0.05 part of citric acid, and the 100 parts of deionized water is used for supplementing.

The preparation method comprises the following specific steps:

weighing 4 parts of snow lotus extract, 5 parts of snow grass extract, 5 parts of nicotinamide, 5 parts of hyaluronic acid, 6 parts of glycerol, 3 parts of hydroxyethyl cellulose, 0.5 part of polyacrylic acid, 0.4 part of p-hydroxyacetophenone and 0.3 part of hydrogenated castor oil. The components are sequentially added into deionized water at 60 ℃, stirred for 2 hours at a constant speed of 3000rpm/min by using a stirrer, then 0.05 part of citric acid is added, and stirring is continued for about 20 minutes. Then 5 parts of PDRN are added and the mixture is made up to 100 parts with deionized water, and stirring is continued until the mixture is uniform. After being stirred uniformly, the mixture is kept stand to room temperature, and the whitening, spot-lightening, moisturizing and skin-tendering essence containing PDRN is obtained.

Test A: the essences described in embodiments 1 and 2 were diluted 1, 3, 5, and 10 times, respectively, and subjected to a tyrosine kinase activity inhibition test, a cell proliferation test, a melanocyte tyrosinase activity inhibition test, and a melanin synthesis inhibition test. The experiment is divided into: a 1-time diluted group, a 3-time diluted group, a 5-time diluted group, a 10-time diluted group, a PDRN-free group (the other components except PDRN are the same as in embodiments 1 and 2), and a commercially available whitening, freckle-removing and moisturizing product group.

And (3) testing B: the essence described in embodiments 1 and 2, the essence without PDRN (except that PDRN is not contained, the other components are the same as those in embodiments 1 and 2), and a moisturizing performance test of a commercially available whitening and freckle removing moisturizing product. The method comprises the following steps: weighing 10g of each product and 10g of glycerol aqueous solution at room temperature, respectively placing in a weighing dish, placing in a constant temperature and humidity box (under constant temperature and humidity conditions: temperature 22 ℃ and relative humidity 40%) with an opening, weighing after the placing time is divided into 6h, 12h, 24h and 36h, and calculating the moisture retention rate. Moisture retention rate is mass after standing/mass before standing × 100%.

And (3) testing C: 30 healthy female volunteers of 20-40 years of average age were selected, and had facial pigmentation and spots. Subjects were randomized into 6 groups: the example 1, 2, 1, 2-PDRN, 2, 1, 2-PDRN, 1 and 2-market whitening and spot-removing products. After cleaning the face with warm water every day, the subject applies appropriate amount of the above cosmetics in groups, once every morning and evening, and continuously uses for 6 weeks. No other cosmetic products were used during the test. The influence of each cosmetic on the skin color is detected once every two weeks through visual observation, photographing record and skin color difference detection by a probe. The skin moisture tester is used for testing the influence of the cosmetics on the moisture content, the elasticity and the quality of the skin once every two weeks. The results show that the cosmetics show whitening, freckle removing, moisturizing and skin tendering effects in different degrees, and the essence solutions of the PDRN-containing examples 1 and 2 have better effects than the corresponding essence solutions without the PDRN and the tested commercially available cosmetics.

Claims (6)

1. The PDRN-containing essence with the functions of whitening, fading spots, moisturizing and tendering skin is characterized by comprising the following raw materials in parts by weight: PDRN1-10 parts, saussurea involucrate extracting solution 5-10 parts, centella asiatica extracting solution 3-8 parts, nicotinamide 3-12 parts, hyaluronic acid 3-6 parts, glycerol 5-10 parts, hydroxyethyl cellulose 2-4 parts, polyacrylic acid 0.5-1 part, p-hydroxyacetophenone 0.3-0.6 part, hydrogenated castor oil 0.2-0.5 part, citric acid 0.05-0.1 part, and deionized water is used for supplementing to 100 parts.

2. The PDRN-containing essence with the functions of whitening, fading spots, moisturizing and tendering skin is characterized by being prepared from the following raw materials in parts by weight: 2 parts of PDRN, 5 parts of saussurea involucrate extract, 6 parts of centella asiatica extract, 5 parts of nicotinamide, 4 parts of hyaluronic acid, 6 parts of glycerol, 3 parts of hydroxyethyl cellulose, 0.5 part of polyacrylic acid, 0.4 part of p-hydroxyacetophenone, 0.3 part of hydrogenated castor oil and 0.05 part of citric acid, and the 100 parts of deionized water is used for supplementing.

3. The PDRN-containing essence with the functions of whitening, fading spots, moisturizing and tendering skin is characterized by being prepared from the following raw materials in parts by weight: 5 parts of PDRN, 4 parts of saussurea involucrate extract, 5 parts of centella asiatica extract, 5 parts of nicotinamide, 5 parts of hyaluronic acid, 6 parts of glycerol, 3 parts of hydroxyethyl cellulose, 0.5 part of polyacrylic acid, 0.4 part of p-hydroxyacetophenone, 0.3 part of hydrogenated castor oil and 0.05 part of citric acid, and the 100 parts of deionized water is used for supplementing.

4. The preparation process of the PDRN-containing essence with the functions of whitening, fading spots, moisturizing and rejuvenating skin as claimed in claim 1, wherein the PDRN is extracted by the following steps: taking out the spermary of the salmon fish, cleaning the spermary with precooled normal saline, placing the cleaned spermary in an extraction buffer solution, and homogenizing and crushing by using a homogenizing crusher; adding proper protease K to enzymolyze protein; thirdly, centrifuging, collecting supernatant, adding saturated sodium chloride solution into the supernatant, mixing uniformly, centrifuging and collecting supernatant containing DNA; adding absolute ethyl alcohol into the collected supernatant, precipitating DNA, and washing the precipitate twice with 75% ethyl alcohol; dissolving the obtained DNA molecules by using deionized water, and carrying out ultrasonic crushing; sixthly, freeze drying to obtain the micromolecule Polydeoxyribonucleotide (PDRN).

5. The preparation process of the PDRN-containing essence with the functions of whitening, fading spots, moisturizing and tendering skin as claimed in claim 1, wherein the preparation methods of the snow lotus extract and the snow grass extract are as follows:

respectively cleaning herba Adonidis and herba Centellae, oven drying, and pulverizing; extracting the crushed powder in a supercritical extraction reactor; the extractant used was ethanol: butanediol (3: 3-8), the temperature is 45-75 ℃, the pressure is 30-40MPa, and the circular extraction is carried out for 3-4 hours; filtering the extractive solution, and concentrating to obtain herba Saussureae Involueratae extractive solution and herba Centellae extractive solution.

6. The preparation process of the PDRN-containing essence with the functions of whitening, lightening spots, moisturizing and rejuvenating skin as claimed in claim 1, wherein the preparation process comprises the following steps:

1) extracting small molecule Polydeoxyribonucleotide (PDRN) from salmon according to the extraction step of PDRN;

2) the preparation method comprises the following steps of weighing the snow lotus herb extract, the centella asiatica extract, the nicotinamide, the hyaluronic acid, the glycerol, the hydroxyethyl cellulose, the polyacrylic acid, the p-hydroxyacetophenone and the hydrogenated castor oil according to the weight part of the raw materials in claim 1, slowly adding the components into deionized water in sequence, stirring for 1-3 hours at the temperature of 60-72 ℃ by using a stirrer, adding the citric acid in corresponding parts by mass, continuously stirring uniformly, standing for 1 hour to be stable, adding the PDRN, fully stirring uniformly, and standing to room temperature to obtain the essence.