CN114067315B - Cell counting method, cell counting device, computer device, and storage medium - Google Patents
Cell counting method, cell counting device, computer device, and storage medium Download PDFInfo
- Publication number
- CN114067315B CN114067315B CN202111236635.8A CN202111236635A CN114067315B CN 114067315 B CN114067315 B CN 114067315B CN 202111236635 A CN202111236635 A CN 202111236635A CN 114067315 B CN114067315 B CN 114067315B
- Authority
- CN
- China
- Prior art keywords
- cell
- consumable
- counting
- type
- light source
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06F—ELECTRIC DIGITAL DATA PROCESSING
- G06F18/00—Pattern recognition
- G06F18/20—Analysing
- G06F18/21—Design or setup of recognition systems or techniques; Extraction of features in feature space; Blind source separation
- G06F18/214—Generating training patterns; Bootstrap methods, e.g. bagging or boosting
Landscapes
- Engineering & Computer Science (AREA)
- Data Mining & Analysis (AREA)
- Theoretical Computer Science (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Artificial Intelligence (AREA)
- Evolutionary Biology (AREA)
- Evolutionary Computation (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present application relates to a cell counting method, apparatus, computer device and storage medium, the method comprising: the cell counting instrument obtains the type of the cell consumable to be detected currently, the cell consumable is determined to be moved to a target position according to the type of the cell consumable, and then cells on the cell consumable are counted on the target position; wherein the cell consumables are of the type including manual consumables or automatic consumables; that is to say, the cell technology appearance in this application embodiment can support the cell counting function of the cell consumptive material of multiple different grade type, has increased the compatibility of cell counting appearance to and the function of extension cell counting appearance, improved the application scope of cell counting appearance greatly, improved flexibility, convenience and the high efficiency that the user carries out cell counting work.
Description
Technical Field
The present application relates to the field of cell counting technologies, and in particular, to a cell counting method, an apparatus, a computer device, and a storage medium.
Background
Cell counting is a more conventional and important link in cell biology research, and under the continuous development of the field of biology research, the requirements on accuracy and high efficiency of cell counting are higher and higher.
In the conventional technology, a cell counting instrument is generally adopted to perform cell counting analysis on a cell sample to be detected, the existing cell counting instrument comprises two types, one type is that the cell sample is manually dyed and then is loaded into a cell consumable, the cell counting instrument performs counting analysis on the dyed cell sample in the cell consumable, the other type is that the cell sample is loaded into the cell consumable, and the cell counting instrument performs automatic dyeing on the cell sample in the cell consumable and performs cell counting analysis after dyeing.
However, the existing cell counting instrument has a single function and cannot meet the current cell counting requirement.
Disclosure of Invention
In view of the above, there is a need to provide a multifunctional cell counting instrument, a cell counting method, a cell counting device, a computer device and a storage medium, which can meet different requirements of cell counting.
In a first aspect, there is provided a cell counting method, which is applied to a cell counter, the method including:
acquiring the type of the cell consumable to be detected currently;
determining to move the cell consumable to a target location according to the type of the cell consumable;
counting cells on the cell consumable at the target location.
In one embodiment, the cell counter comprises a light source and at least one photodiode, wherein an illumination signal of the light source is received by the photodiode after passing through the cell consumable; obtaining the type of the cell consumable to be detected currently, including:
acquiring the intensity of an illumination signal received by a photodiode;
and determining the type of the cell consumable according to the intensity of the illumination signal.
In one embodiment, the cell counter has a plurality of light sources, and the acquiring of the intensity of the illumination signal received by the photodiode includes:
and controlling the plurality of light sources to sequentially irradiate the cell consumables to obtain a plurality of illumination signal intensities received by the photodiode.
In one embodiment, determining the type of cell consumable based on the intensity of the illumination signal comprises:
determining the arrangement sequence of the intensities of a plurality of illumination signals according to the light emitting sequence of a plurality of light sources to form an illumination intensity combination;
determining the type of the cell consumable according to the illumination intensity combination and the first corresponding relation; wherein the first corresponding relationship comprises corresponding relationships between different light intensity combinations and different types of cell consumables.
In one embodiment, before acquiring the type of cell consumable currently to be tested, the method further comprises:
sending a starting instruction to the light source to control the light source to emit light;
and determining whether the cell consumable to be detected exists on the cell counter according to the intensity of the illumination signal received by the photodiode.
In one embodiment, the method further comprises:
under the condition that the cell counting instrument is determined to have cell consumables to be detected, acquiring identification of the cell consumables, and sending consumable data requests to a server, wherein the consumable data requests carry the identification;
and receiving consumable data which is sent by the server and corresponds to the identification, and determining whether the cell consumable is used or not according to the consumable data.
In one embodiment, the cell consumables are of the type including manual consumables or automatic consumables.
In one embodiment, determining to move the cell consumable to the target location according to the type of the cell consumable includes:
under the condition that the cell consumable is a manual consumable, moving the cell consumable to a cell counting position;
in the case where the cell consumable is an automatic consumable, the cell consumable is moved to a cell staining position.
In one embodiment, counting cells on a cell consumable at a target location comprises:
under the condition that the cell consumable is positioned at the cell counting position, sequentially controlling a plurality of light sources to irradiate cell samples to be detected in each detection channel of the cell consumable;
when the light source irradiates the cell sample to be detected in the detection channel, controlling a focusing assembly on the cell counter to focus to a target focal length;
acquiring a target cell image of the cell sample in the detection channel under the irradiation of the light source based on the target focal length;
and carrying out image analysis processing on the target cell image to obtain a cell counting result.
In one embodiment, counting cells on a cell consumable at a target location comprises:
under the condition that the cell consumable is located at the cell staining position, controlling a blending component on a cell counter to sequentially stain and blend cell samples to be detected in all detection channels of the cell consumable to obtain the stained and blended cell samples in all detection channels;
sequentially controlling a plurality of light sources to irradiate the cell samples which are dyed and uniformly mixed in each detection channel, and acquiring target cell images of the cell samples in each detection channel;
and carrying out image analysis processing on the target cell images of the cell samples in the detection channels to obtain cell counting results of the cell samples in the detection channels.
In a second aspect, there is provided a cell counting device, which is applied to a cell counter, the device comprising:
the acquisition module is used for acquiring the type of the cell consumable to be detected currently;
a first determining module for determining to move the cell consumable to a target location according to the type of the cell consumable;
and the counting module is used for counting the cells on the cell consumable at the target position.
In a third aspect, a computer device is provided, comprising a memory and a processor, the memory storing a computer program, the processor implementing the following steps when executing the computer program:
acquiring the type of the cell consumable to be detected currently;
determining to move the cell consumable to a target location according to the type of the cell consumable;
the cells on the cell consumable are counted at the target location.
In a fourth aspect, there is provided a computer readable storage medium having stored thereon a computer program which, when executed by a processor, performs the steps of:
acquiring the type of the cell consumable to be detected currently;
determining to move the cell consumable to a target location according to the type of the cell consumable;
counting cells on the cell consumable at the target location.
According to the cell counting method, the cell counting device, the computer equipment and the storage medium, the cell counter obtains the type of the cell consumable to be detected currently, the cell consumable is determined to be moved to the target position according to the type of the cell consumable, and then the cells on the cell consumable are counted at the target position; that is to say, the cell technology appearance in this application embodiment can support the cell counting function of the cell consumptive material of multiple different grade type, has increased the compatibility of cell counting appearance to and the function of extension cell counting appearance, improved the application scope of cell counting appearance greatly, improved flexibility, convenience and the high efficiency that the user carries out cell counting work.
Drawings
FIG. 1 is a schematic flow chart of a cell counting method in one embodiment;
FIG. 2 is a schematic flow chart of a cell counting method according to another embodiment;
FIG. 3 is a schematic flow chart of a cell counting method according to another embodiment;
FIG. 4 is a schematic flow chart of a cell counting method according to another embodiment;
FIG. 5 is a schematic flow chart of a cell counting method according to another embodiment;
FIG. 6 is a schematic flow chart of a cell counting method according to another embodiment;
FIG. 7 is a schematic flow chart of a cell counting method according to another embodiment;
FIG. 8 is a block diagram showing the structure of a cell counting apparatus according to an embodiment;
FIG. 9 is a diagram illustrating an internal structure of a computer device according to an embodiment.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the present application is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.
The cell counting method provided by the application can be applied to computer equipment for cell counting, and the computer equipment can be a cell counter or any type of cell detector with a cell counting function; the cell counting instrument or the cell detecting instrument can support cell counting on the manually dyed cell samples and also support counting on the automatically dyed cell samples; that is, the cell counting instrument or the cell detecting instrument may be provided with manual consumables or automatic consumables, wherein the manual consumables contain the cell sample after being manually stained, and the automatic consumables contain the cell sample without being stained; optionally, the cell counter or the cell detector may be used to independently place manual consumables or automatic consumables, or may be used to place manual consumables and automatic consumables simultaneously.
Alternatively, manual and automatic consumables may include multiple channels, for example: the manual consumables can be 8 channels, and the automatic consumables can be 24 channels, and it should be noted that, this application embodiment does not limit the number of channels of different consumables.
In one embodiment, as shown in fig. 1, a cell counting method is provided, which is described by taking the application of the method to the cell counter as an example, and comprises the following steps:
Alternatively, the type of cell consumables may include manual consumables, automatic consumables, semi-automatic consumables, coarse detection consumables, fine detection consumables, and the like.
Optionally, in the case of placing the cell consumable to be detected into the cell counting instrument, when the cell counting instrument is started to work, the cell counting instrument may first obtain the type of the cell consumable to be detected that is currently placed into the cell counting instrument; optionally, each cell consumable may be provided with an identification code for identifying the type of the cell consumable, the identification code may be a two-dimensional code, a bar code, or the like, and the cell counter may obtain the type of the cell consumable to be detected by scanning and obtaining the identification code on the cell consumable to be detected; optionally, the cell counter may further include a human-computer interface, and the type of the cell consumable to be detected may be determined by obtaining a trigger operation (inputting the type of the cell consumable to be detected) of the user in the human-computer interface; of course, other cell consumable types may be obtained, which is not limited in the embodiments of the present application.
Optionally, since the cell sample in the manual consumable has been cell-stained and the cell sample in the automatic consumable is a cell sample that has not been stained, the cell counting instrument has a difference in the process of counting the cell sample to be tested in each channel in the consumable for the manual consumable and the automatic consumable, and at least for the automatic consumable, the cell staining process needs to be performed on the cell sample to be tested in each channel in the automatic consumable, and then the cell counting process is performed; therefore, the detection counting processes corresponding to the manual consumable and the automatic consumable are different; alternatively, in the cytometer, for different types of cell consumables (manual consumables or automatic consumables), target positions corresponding to the different types of cell consumables may be preset, that is, a corresponding relationship between the different types of consumables and the target positions of the types of consumables is preset; and determining to move the cell consumable to the target position according to the type of the cell consumable and the corresponding relation.
Optionally, in the case that the cell consumable is a manual consumable, the cell consumable can be moved to a cell counting position to directly perform a cell counting process; under this cell consumptive material is automatic consumptive material's the condition, can remove this cell consumptive material to cell staining position, carry out cell staining process, after dyeing, can carry out the cell count process to the cell after dyeing. Optionally, the cell counting procedure may include acquiring a cell image in at least one field position of the cell channel to be detected, performing image analysis on the cell image in each field position to obtain an image analysis result in each field position, and then determining a cell counting result of the cell sample to be detected in the cell channel to be detected according to the image analysis result in each field position; similarly, cell counting results of the cell sample to be tested in other cell channels of the cell consumable can be obtained; finally, after all channels of the cell consumable are detected, outputting a detection result; optionally, when the cell image at each visual field position is acquired, the cell image at the visual field position may be focused to adjust the image to the clearest position, so as to obtain a cell image with a definition meeting a preset requirement. Optionally, the cell staining process may include performing a blending operation on the cell sample to be detected in each cell channel to be detected to ensure that cells in the cell sample to be detected are uniformly distributed, and then performing cell staining on the blended cell sample to be detected to obtain a stained cell sample to be detected.
In step 103, the cells on the cell consumable are counted at the target location.
Optionally, after the cell consumable is moved to the target position, the cell sample to be measured on the cell consumable is counted by using the cell counting process corresponding to the target position, so as to obtain a counting result of the cell sample to be measured in each channel of the cell consumable.
In the cell counting method, the cell counting instrument acquires the type of the cell consumable to be detected currently, and determines to move the cell consumable to a target position according to the type of the cell consumable, and then counts the cells on the cell consumable at the target position; wherein the cell consumables are of the type including manual consumables or automatic consumables; that is to say, cell technology appearance in this application embodiment can support the cell count function of the cell consumptive material of multiple different grade type, has increased the compatibility of cell count appearance to and the function of extension cell count appearance, improved the application scope of cell count appearance greatly, improved flexibility, convenience and the high efficiency that the user carries out cell count work.
FIG. 2 is a schematic flow chart of a cell counting method in another embodiment. The embodiment relates to one optional implementation process of acquiring the type of a cell consumable to be detected by a cell counter through an illumination principle; on the basis of the above embodiment, as shown in fig. 2, the step 101 includes:
The cell counter comprises a light source and at least one photodiode, and an illumination signal of the light source is received by the photodiode after passing through the cell consumable. For different types of cell consumables, the cell consumables are made of different materials, and after the same illumination signal passes through the cell consumables of different types, the intensity of the illumination signal received by the photodiode is different.
Optionally, after the cell consumable to be detected is put into the cell counting instrument and the cell counting instrument is started to work, the cell counting instrument can control the light source to be turned on and control the light source to irradiate the cell consumable to be detected, so that each photodiode can receive the illumination signal intensity after passing through the cell consumable, and further, each photodiode can transmit the received illumination signal intensity to the processor of the cell counting instrument, so that the processor of the cell counting instrument obtains the illumination signal intensity after the light source received by each photodiode passes through the cell consumable to be detected.
And step 202, determining the type of the cell consumable according to the intensity of the illumination signal.
Optionally, the corresponding relationship between different types of cell consumables and different illumination signal intensities can be preset in the cell counting instrument; after the cell counting instrument acquires the intensity of the illumination signal received by each photodiode after passing through the cell consumable to be detected, the type of the cell consumable to be detected can be determined according to the intensity of the illumination signal and the corresponding relation. Optionally, the intensity of the illumination signal received by one photodiode of the at least one photodiode and the corresponding relationship may be selected to determine the type of the cell consumable; or determining the average illumination signal intensity according to the illumination signal intensity received by the at least one photodiode, and then determining the type of the cell consumable according to the average illumination signal intensity and the corresponding relationship; this is not a limitation of the present application.
In this embodiment, set up light source and at least one photodiode in the cell count appearance, through the illumination signal intensity who acquires the illumination signal behind the cell sample that the light source that each photodiode received sent through waiting to detect, and confirm the type of cell consumptive material according to this illumination signal intensity, can realize the judgement to cell consumptive material type through simpler structure, the structure of cell count appearance has been simplified, be favorable to the miniaturization of cell count appearance, can also improve the accuracy of judging the cell consumptive material type.
In an alternative embodiment of the present application, the light source of the cytometer may be multiple, and each light source may be of a different type, for example: a bright field light source, a fluorescent light source and the like, wherein the fluorescent light source can be an AO light source, a PI light source and the like; the type of the cell consumable can be comprehensively judged by controlling a plurality of light sources to respectively irradiate the cell consumable to be detected; optionally, the step 201 of "acquiring the intensity of the illumination signal received by the photodiode" may further include: the cell consumable is irradiated by controlling the plurality of light sources in sequence to obtain a plurality of illumination signal intensities received by the photodiode, that is, each light source can be controlled to be started in sequence and irradiated on the cell consumable, so that the photodiode receives the illumination signal intensity of the illumination signal transmitted by the light source after passing through the cell consumable, the light source is closed, and then the next light source is controlled to be started, so that the photodiode receives the illumination signal intensity of the illumination signal transmitted by the next light source after passing through the cell consumable, and so on until the photodiode collects the illumination signal intensity of the illumination signal transmitted by all the light sources after passing through the cell consumable.
Optionally, in the case of obtaining the illumination signal intensities under irradiation of a plurality of light sources, the cell counter may calculate an average illumination signal intensity of the illumination signal intensities under irradiation of the plurality of light sources for the illumination signal intensities corresponding to different light sources received by the same photodiode, and then determine the type of the cell consumable according to the average illumination signal intensity and the correspondence relationship in step 202.
In this embodiment, this cell count appearance's light source is a plurality of, shines the cell consumptive material in proper order through controlling a plurality of light sources, obtains a plurality of illumination signal intensity that photodiode received, then, confirms the type of cell consumptive material according to this a plurality of illumination signal intensity, can improve the accuracy of judging the cell consumptive material type.
FIG. 3 is a schematic flow chart of a cell counting method in another embodiment. The embodiment relates to a cell counting instrument which is provided with a plurality of light sources, and under the condition that a plurality of illumination signal intensities received by a photodiode under the irradiation of the plurality of light sources are obtained, one optional implementation process of determining the type of a cell consumable is determined according to the illumination signal intensities; on the basis of the above embodiment, as shown in fig. 3, the step 202 includes:
That is, in this embodiment, the illumination signal intensity received by the photodiode through the cell consumable under the illumination of each light source is obtained, and the illumination intensity combination is obtained.
Wherein the first corresponding relationship comprises corresponding relationships between different light intensity combinations and different types of cell consumables.
In this embodiment, the cell counter determines an arrangement order of a plurality of illumination signal intensities according to a light emitting order of a plurality of light sources to form an illumination intensity combination, and then determines a type of the cell consumable according to the illumination intensity combination and a first corresponding relationship; wherein the first correspondence comprises correspondence between different combinations of illumination intensities and different types of cell consumables; can improve the accuracy of judging the type of the cell consumable.
FIG. 4 is a schematic flow chart of a cell counting method according to another embodiment. The embodiment relates to an optional implementation process for judging whether the cell consumable to be detected exists on a cell counter before the type of the cell consumable to be detected is obtained; on the basis of the above embodiment, as shown in fig. 4, the method further includes:
Alternatively, the light source may be any one of the above-described plurality of light sources. After the cell counting instrument is started to work, the cell counting instrument can firstly judge whether a preset position (a position for placing cell consumables) is a cell consumable to be detected or not; alternatively, the detection may be performed by using a photoelectric principle, that is, the light source may be controlled to emit light by sending a turn-on command to the light source, and the light emitted by the light source is controlled to irradiate the preset position.
And step 402, determining whether the cell consumable to be detected exists on the cell counter according to the intensity of the illumination signal received by the photodiode.
Because the intensity of the light signal of the light source directly received by the photodiode is greatly different from the intensity of the light signal of the light source after the light signal of the light source passes through the cell consumables, the intensity ranges of the light signals corresponding to the cell consumables and the cell-free consumables can be preset to obtain a preset mapping relation; after the cell counter acquires the intensity of the illumination signal received by the photodiode, the current intensity of the illumination signal is judged to be within the intensity range of the illumination signal with cell consumables or within the intensity range of the illumination signal without the cell consumables according to the intensity of the illumination signal and the preset mapping relation, so that whether the cell consumables to be detected exist on the cell counter is judged.
In this embodiment, the cell counting instrument can send the opening instruction to the light source before obtaining the type of the cell consumptive material that waits to detect at present, and the control light source is luminous, and whether there is the cell consumptive material that waits to detect on the definite cell counting instrument according to the illumination signal intensity that photodiode received, can avoid carrying out the condition of invalid detection under the condition that no cell consumptive material was put into, perhaps appear detecting unusual problem, improves the intelligence and the high efficiency of this cell counting instrument.
FIG. 5 is a schematic flow chart of a cell counting method according to another embodiment. The embodiment relates to a method for judging whether a cell consumable is used or not before acquiring the type of the cell consumable to be detected currently; on the basis of the above embodiment, as shown in fig. 5, the method further includes:
Optionally, the cell counting instrument may further determine whether the cell consumable to be detected has been used or not, that is, determine whether the cell consumable has been detected or not, under the condition that it is determined that the cell consumable to be detected currently exists, so as to prevent a problem of repeated detection of the cell consumable; optionally, the cell counter may obtain an identifier of the cell consumable, and send a consumable data request carrying the identifier to the server; the identifier of the cell consumable may be a code (e.g., a unique ID) of the cell consumable, or may be a name of the cell consumable, which is not limited in this embodiment; in addition, the mode of obtaining the identification of the cell consumable can be through the mode of scanning the two-dimensional code or the code on the cell consumable, also can be through the identification of human-computer interface input this cell consumable, and this application embodiment does not limit this.
Optionally, when the cell consumable is detected and not detected, the corresponding consumable data for the identifier is different, for example: the cell consumable can be marked to be detected or not detected through different character strings, for example, Y indicates that the cell consumable is detected, and N indicates that the cell consumable is not detected; the corresponding consumable data can be the detected result under the condition that the cell consumable is detected, and the corresponding consumable data is empty under the condition that the cell consumable is not detected; the embodiment of the present application does not limit this. It should be noted that, after detecting the first cell sample to be detected through the cell consumable, the consumable data corresponding to the identifier of the cell consumable indicates that the cell consumable is used, and after cleaning the cell consumable, after placing the second cell sample to be detected, the consumable data corresponding to the identifier of the cell consumable needs to be updated to be the consumable data when the consumable data is not detected (i.e. not used), so that the cell counting instrument can execute the cell counting process on the cell consumable placed in the second cell sample to be detected, and the cell counting result of the second cell sample to be detected is obtained.
Based on this, the cytometer may determine whether the cell consumable is used according to the consumable data by receiving the consumable data corresponding to the identifier transmitted by the server. Under the condition that the cell consumable is not used, the cell counting method can be continuously executed, namely the type of the cell consumable to be detected currently is obtained, the cell consumable is determined to be moved to a target position according to the type of the cell consumable, and the cells on the cell consumable are counted at the target position; when the cell consumable is used, reminding information can be output to remind a user that the cell consumable is used, and the user is advised to put the unused cell consumable to be detected again and quit the operation.
In this embodiment, the cell count appearance has the cell consumptive material's that waits to detect the condition on confirming the cell count appearance, acquire the sign of cell consumptive material, and send the consumptive material data request that carries this sign to the server, then, receive the consumptive material data that corresponds with this sign that the server sent, and confirm whether this cell consumptive material is used according to this consumptive material data, in order to avoid carrying out the problem that repeated detection leads to the invalid work of cell count appearance to used cell consumptive material, can improve the intelligence and the high efficiency of this cell count appearance.
FIG. 6 is a flow chart illustrating a cell counting method according to another embodiment. The embodiment relates to one optional implementation process of counting the cells on the cell consumable at the target position under the condition that the cell consumable is positioned at the cell counting position; on the basis of the above embodiment, as shown in fig. 6, the step 103 includes:
Wherein the cell consumable comprises a plurality of detection channels, optionally the manual consumable may comprise 8 detection channels; the light source may include a bright field light source and a fluorescent light source, which may include an AO light source and a PI light source.
Optionally, under the condition that the cell consumable is located at the cell counting position, for each detection channel to be detected on the cell consumable, each light source can be sequentially controlled to irradiate onto the cell sample to be detected in the detection channel by controlling the light source switching module; for example: controlling a bright field light source to move to the position of the detection channel by controlling a light source switching module, and starting the bright field light source to enable an illumination signal emitted by the bright field light source to irradiate a cell sample to be detected in the detection channel; after the preset time, closing and removing the bright field light source, controlling the AO light source to move to the position of the detection channel through the control light source switching module, and opening the AO light source to enable an illumination signal emitted by the AO light source to irradiate on a cell sample to be detected in the detection channel; after the preset time, the AO light source is closed and removed, the PI light source is controlled to move to the position of the detection channel through the control light source switching module, and the PI light source is started, so that the illumination signal emitted by the PI light source can irradiate on the cell sample in the detection channel.
And obtaining a cell image of the cell sample to be detected, wherein the definition of the cell image is the highest under the target focal length.
Optionally, the target focal length may be preset, or the target focal length corresponding to the cell image with the highest definition may be obtained by successively iterating the definitions of the cell images obtained at different focal lengths; the method for acquiring the target focal length is not limited in the application.
Optionally, after the focusing is controlled to the target focal length by controlling the focusing assembly, controlling the shooting assembly to shoot a target cell image of the cell sample in the detection channel under the irradiation of the current light source; optionally, a plurality of target cell images of the cell sample in the detection channel at different visual field positions can be acquired by controlling the movement of the light source.
And step 604, performing image analysis processing on the target cell image to obtain a cell counting result.
Optionally, a preset image analysis algorithm is adopted to perform image analysis processing on the target cell image, so as to obtain a cell count result of the cell sample in the detection channel, where the cell count result may include at least one of a total number of cells, a number of dead cells, or a number of live cells.
Based on the above steps, after cell analysis results of one detection channel of the cell consumable under the respective irradiation of the plurality of light sources are obtained, the cell consumable is controlled to move to the next detection channel, the plurality of light sources are continuously controlled and switched to obtain cell analysis results of the next detection channel under the respective irradiation of the plurality of light sources, and so on until cell analysis results of each detection channel of the cell consumable under the respective irradiation of the plurality of light sources are obtained, finally, a final cell analysis result of the cell consumable is output, the final cell analysis result includes cell analysis results corresponding to each detection channel, and the cell analysis result corresponding to each detection channel includes cell analysis results under different light sources.
In this embodiment, under the condition that the cell consumable is located at the cell counting position, the light sources are sequentially controlled to irradiate the cell sample to be detected in each detection channel of the cell consumable; when the light source irradiates the cell sample to be detected in the detection channel, controlling a focusing assembly on the cell counter to focus to a target focal length; acquiring a target cell image of the cell sample in the detection channel under the irradiation of the light source based on the target focal length; carrying out image analysis processing on the target cell image to obtain a cell counting result; by carrying out focusing treatment on the cell samples to be detected in each detection channel, the cell images with higher definition can be obtained, and the accuracy of the analysis results of the cell images can be improved, so that the comprehensive performance of the cell counter is improved.
FIG. 7 is a schematic flow chart of a cell counting method according to another embodiment. The embodiment relates to one optional implementation process of counting the cells on the cell consumable at the target position under the condition that the cell consumable is positioned at the cell staining position; on the basis of the above embodiment, as shown in fig. 7, the step 103 includes:
and 701, controlling a blending component on the cell counting instrument to sequentially dye and blend cell samples to be detected in all detection channels of the cell consumable under the condition that the cell consumable is located at the cell dyeing position, so as to obtain the cell samples dyed and blended in all the detection channels.
Optionally, the mixing assembly may include a plurality of mixing subassemblies, one mixing subassembly corresponding to one detection channel, and the mixing subassembly may include a blowing and sucking assembly, an air extracting assembly, a first micro flow channel and a second micro flow channel; the detection channel is connected with the blowing and sucking component through a first micro-channel, the detection channel is connected with the air pumping component through a second micro-channel, and dye is arranged in the second micro-channel; the cell samples in the detection channel are uniformly mixed by controlling the blowing and sucking assembly, and after uniform mixing, the cell samples in the detection channel can be sucked to the second micro flow channel by controlling the air pumping assembly, so that the cell samples and the dye in the second micro flow channel are fused to realize dyeing of the cell samples, and the dyed and uniformly mixed cell samples are obtained.
And 702, sequentially controlling a plurality of light sources to irradiate the cell samples which are dyed and uniformly mixed in each detection channel, and acquiring target cell images of the cell samples in each detection channel.
Optionally, after the cell sample in each detection channel is dyed and uniformly mixed, the obtained dyed and uniformly mixed cell sample is located in a second micro-channel connected with the detection channel, and the second micro-channel comprises a detection window which can be used for shooting; and aiming at each detection channel, sequentially controlling a plurality of light sources to move to the detection window, and controlling the shooting assembly to obtain a plurality of target cell images corresponding to the stained and uniformly mixed cell samples in the detection window. Optionally, after the light source is controlled to irradiate the detection window, a focusing assembly on the cell counter may be controlled to focus to a target focal length, which may specifically refer to the related discussion in step 602, and is not described herein again; and then, based on the target focal length, controlling the shooting assembly to obtain a target cell image corresponding to the cell sample after dyeing and uniform mixing in the detection window, wherein the definition of the target cell image obtained after focusing is higher.
And 703, performing image analysis processing on the target cell image of the cell sample in each detection channel to obtain a cell counting result of the cell sample in each detection channel.
Optionally, performing image analysis processing on the target cell image of the cell sample in each detection channel by using a preset image analysis algorithm to obtain a cell count result of the cell sample in each detection channel, where the cell count result may include at least one of the total number of cells, the number of dead cells, or the number of live cells; and finally, combining the cell counting results of the cell samples in the detection channels into a final cell analysis result of the cell consumable.
In this embodiment, under the condition that the cell consumable is located at the cell staining position, controlling a blending component on the cell counter to sequentially stain and blend cell samples to be detected in each detection channel of the cell consumable to obtain the stained and blended cell samples in each detection channel; sequentially controlling a plurality of light sources to irradiate the cell samples which are dyed and uniformly mixed in each detection channel, and acquiring target cell images of the cell samples in each detection channel; carrying out image analysis processing on the target cell image of the cell sample in each detection channel to obtain a cell counting result of the cell sample in each detection channel; the cell counting appearance in this application can realize the automatic dyeing of cell sample to automatic consumptive material promptly, reduces the complexity of artifical dyeing, in addition, carries out focus treatment through the cell sample to after dyeing, obtains the cell image that the definition is higher, can improve the accuracy to the analysis result of this cell image to this comprehensive properties who improves the cell counting appearance.
It should be understood that although the various steps in the flow charts of fig. 1-7 are shown in order as indicated by the arrows, the steps are not necessarily performed in order as indicated by the arrows. The steps are not performed in the exact order shown and described, and may be performed in other orders, unless explicitly stated otherwise. Moreover, at least some of the steps in fig. 1-7 may include multiple steps or multiple stages, which are not necessarily performed at the same time, but may be performed at different times, which are not necessarily performed in sequence, but may be performed in turn or alternately with other steps or at least some of the other steps.
In one embodiment, as shown in fig. 8, there is provided a cell counting apparatus comprising: an acquisition module 801, a first determination module 802, and a counting module 803, wherein:
an obtaining module 801, configured to obtain a type of a cell consumable to be currently detected;
a first determining module 802, configured to determine to move the cell consumable to a target location according to the type of the cell consumable;
a counting module 803, configured to count the cells on the cell consumable at the target location.
In one embodiment, the cell counter comprises a light source and at least one photodiode, wherein an illumination signal of the light source is received by the photodiode after passing through the cell consumable; the acquiring module 801 includes an acquiring unit and a determining unit; the acquisition unit is used for acquiring the intensity of an illumination signal received by the photodiode; and the determining unit is used for determining the type of the cell consumable according to the intensity of the illumination signal.
In one embodiment, the cell counter includes a plurality of light sources, and the acquiring unit is specifically configured to control the plurality of light sources to sequentially irradiate the cell consumable to obtain a plurality of illumination signal intensities received by the photodiode.
In one embodiment, the determining unit is specifically configured to determine an arrangement order of the intensities of the multiple illumination signals according to a light emitting order of the multiple light sources, so as to form an illumination intensity combination; determining the type of the cell consumable according to the illumination intensity combination and the first corresponding relation; wherein the first correspondence includes correspondence between different combinations of illumination intensities and different types of cell consumables.
In one embodiment, the device further comprises a first sending module and a second determining module; the first sending module is used for sending a starting instruction to the light source to control the light source to emit light before the type of the cell consumable to be detected is acquired; and the second determining module is used for determining whether the cell consumable to be detected exists on the cell counter according to the intensity of the illumination signal received by the photodiode.
In one embodiment, the apparatus further comprises a second sending module and a third determining module; the second sending module is used for acquiring the identification of the cell consumable under the condition that the cell consumable to be detected exists on the cell counter, and sending a consumable data request to the server, wherein the identification is carried in the consumable data request; and the third determining module is used for receiving the consumable data which is sent by the server and corresponds to the identification, and determining whether the cell consumable is used or not according to the consumable data.
In one embodiment, the type of cell consumable includes a manual consumable or an automatic consumable.
In one embodiment, the first determining module is specifically configured to move the cell consumable to a cell counting position when the cell consumable is a manual consumable; in the case where the cell consumable is an automatic consumable, the cell consumable is moved to a cell staining position.
In one embodiment, the counting module 803 is specifically configured to sequentially control a plurality of light sources to irradiate the cell sample to be detected in each detection channel of the cell consumable when the cell consumable is located at the cell counting position; when a light source irradiates a cell sample to be detected in the detection channel, controlling a focusing assembly on the cell counter to focus to a target focal length; acquiring a target cell image of the cell sample in the detection channel under the irradiation of the light source based on the target focal length; and carrying out image analysis processing on the target cell image to obtain a cell counting result.
In one embodiment, the counting module 803 is specifically configured to, when the cell consumable is located at the cell staining position, control a blending component on the cell counter to sequentially stain and blend the cell samples to be detected in each detection channel of the cell consumable, so as to obtain the stained and blended cell samples in each detection channel; sequentially controlling a plurality of light sources to irradiate the cell samples which are dyed and uniformly mixed in each detection channel, and acquiring target cell images of the cell samples in each detection channel; and carrying out image analysis processing on the target cell images of the cell samples in the detection channels to obtain cell counting results of the cell samples in the detection channels.
For the specific definition of the cell counting device, reference may be made to the above definition of the cell counting method, which is not described herein again. The various modules in the above described cell counting apparatus may be implemented in whole or in part by software, hardware, and combinations thereof. The modules can be embedded in a hardware form or independent of a processor in the computer device, and can also be stored in a memory in the computer device in a software form, so that the processor can call and execute operations corresponding to the modules.
In one embodiment, a computer device is provided, which may be a cytometer, the internal structure of which may be as shown in fig. 9. The computer device comprises a processor, a memory, a communication interface, a display screen and an input device which are connected through a system bus. Wherein the processor of the computer device is configured to provide computing and control capabilities. The memory of the computer device comprises a nonvolatile storage medium and an internal memory. The non-volatile storage medium stores an operating system and a computer program. The internal memory provides an environment for the operation of an operating system and computer programs in the non-volatile storage medium. The communication interface of the computer device is used for carrying out wired or wireless communication with an external terminal, and the wireless communication can be realized through WIFI, an operator network, NFC (near field communication) or other technologies. The computer program is executed by a processor to implement a method of cell counting. The display screen of the computer equipment can be a liquid crystal display screen or an electronic ink display screen, and the input device of the computer equipment can be a touch layer covered on the display screen, a key, a track ball or a touch pad arranged on the shell of the computer equipment, an external keyboard, a touch pad or a mouse and the like.
Those skilled in the art will appreciate that the architecture shown in fig. 9 is merely a block diagram of some of the structures associated with the disclosed aspects and is not intended to limit the computing devices to which the disclosed aspects apply, as particular computing devices may include more or less components than those shown, or may combine certain components, or have a different arrangement of components.
In one embodiment, a computer device is provided, comprising a memory and a processor, the memory having a computer program stored therein, the processor implementing the following steps when executing the computer program:
obtaining the type of the cell consumable to be detected currently;
determining to move the cell consumable to a target location according to the type of the cell consumable;
the cells on the cell consumable are counted at the target location.
In one embodiment, the processor when executing the computer program further performs the steps of: the cell counter comprises a light source and at least one photodiode, wherein an illumination signal of the light source is received by the photodiode after passing through the cell consumables; acquiring the intensity of an illumination signal received by a photodiode; and determining the type of the cell consumable according to the intensity of the illumination signal.
In one embodiment, the processor when executing the computer program further performs the steps of: the cell counter has multiple light sources, and the multiple light sources are controlled to irradiate cell consumables in sequence to obtain multiple light signal intensities received by the photodiodes.
In one embodiment, the processor, when executing the computer program, further performs the steps of: determining the arrangement sequence of the intensities of the plurality of illumination signals according to the light-emitting sequence of the plurality of light sources to form an illumination intensity combination; determining the type of the cell consumable according to the illumination intensity combination and the first corresponding relation; wherein the first correspondence includes correspondence between different combinations of illumination intensities and different types of cell consumables.
In one embodiment, the processor, when executing the computer program, further performs the steps of: before the type of the cell consumable to be detected is obtained, sending a starting instruction to a light source to control the light source to emit light; and determining whether the cell consumable to be detected exists on the cell counter according to the intensity of the illumination signal received by the photodiode.
In one embodiment, the processor, when executing the computer program, further performs the steps of: under the condition that the cell counting instrument is determined to have the cell consumable to be detected, acquiring an identifier of the cell consumable, and sending a consumable data request to a server, wherein the identifier is carried in the consumable data request; and receiving consumable data which is sent by the server and corresponds to the identification, and determining whether the cell consumable is used or not according to the consumable data.
In one embodiment, the processor when executing the computer program further performs the steps of: the cell consumables may be of the type including manual or automatic consumables.
In one embodiment, the processor, when executing the computer program, further performs the steps of: moving the cell consumable to a cell counting position under the condition that the cell consumable is a manual consumable; in the case where the cell consumable is an automated consumable, the cell consumable is moved to a cell staining site.
In one embodiment, the processor when executing the computer program further performs the steps of: under the condition that the cell consumable is positioned at the cell counting position, sequentially controlling a plurality of light sources to irradiate cell samples to be detected in each detection channel of the cell consumable; when the light source irradiates the cell sample to be detected in the detection channel, controlling a focusing assembly on the cell counter to focus to a target focal length; acquiring a target cell image of the cell sample in the detection channel under the irradiation of the light source based on the target focal length; and carrying out image analysis processing on the target cell image to obtain a cell counting result.
In one embodiment, the processor when executing the computer program further performs the steps of: under the condition that the cell consumable is located at the cell dyeing position, controlling a blending component on the cell counter to sequentially dye and blend cell samples to be tested in each detection channel of the cell consumable to obtain the cell samples dyed and blended in each detection channel; sequentially controlling a plurality of light sources to irradiate the cell samples which are dyed and uniformly mixed in each detection channel, and acquiring target cell images of the cell samples in each detection channel; and carrying out image analysis processing on the target cell image of the cell sample in each detection channel to obtain a cell counting result of the cell sample in each detection channel.
In one embodiment, a computer-readable storage medium is provided, on which a computer program is stored which, when executed by a processor, performs the steps of:
acquiring the type of the cell consumable to be detected currently;
determining to move the cell consumable to a target location according to the type of the cell consumable;
the cells on the cell consumable are counted at the target location.
In one embodiment, the computer program when executed by the processor further performs the steps of: the cell counter comprises a light source and at least one photodiode, wherein an illumination signal of the light source is received by the photodiode after passing through the cell consumable; acquiring the intensity of an illumination signal received by a photodiode; and determining the type of the cell consumable according to the intensity of the illumination signal.
In one embodiment, the computer program when executed by the processor further performs the steps of: the cell counter has multiple light sources, and the multiple light sources are controlled to irradiate cell consumables in sequence to obtain multiple light signal intensities received by the photodiodes.
In one embodiment, the computer program when executed by the processor further performs the steps of: determining the arrangement sequence of the intensities of a plurality of illumination signals according to the light emitting sequence of a plurality of light sources to form an illumination intensity combination; determining the type of the cell consumable according to the illumination intensity combination and the first corresponding relation; wherein the first corresponding relationship comprises corresponding relationships between different light intensity combinations and different types of cell consumables.
In one embodiment, the computer program when executed by the processor further performs the steps of: before the type of the cell consumable to be detected is obtained, sending a starting instruction to a light source to control the light source to emit light; and determining whether the cell counting instrument has cell consumables to be detected according to the intensity of the illumination signal received by the photodiode.
In one embodiment, the computer program when executed by the processor further performs the steps of: under the condition that the cell counting instrument is determined to have the cell consumable to be detected, acquiring an identifier of the cell consumable, and sending a consumable data request to a server, wherein the identifier is carried in the consumable data request; and receiving consumable data which is sent by the server and corresponds to the identification, and determining whether the cell consumable is used or not according to the consumable data.
In one embodiment, the computer program when executed by the processor further performs the steps of: the cell consumables may be of the type including manual or automatic consumables.
In one embodiment, the computer program when executed by the processor further performs the steps of: under the condition that the cell consumable is a manual consumable, moving the cell consumable to a cell counting position; in the case where the cell consumable is an automated consumable, the cell consumable is moved to a cell staining site.
In one embodiment, the computer program when executed by the processor further performs the steps of: under the condition that the cell consumable is positioned at the cell counting position, sequentially controlling a plurality of light sources to irradiate cell samples to be detected in each detection channel of the cell consumable; when a light source irradiates a cell sample to be detected in the detection channel, controlling a focusing assembly on the cell counter to focus to a target focal length; acquiring a target cell image of the cell sample in the detection channel under the irradiation of the light source based on the target focal length; and carrying out image analysis processing on the target cell image to obtain a cell counting result.
In one embodiment, the computer program when executed by the processor further performs the steps of: under the condition that the cell consumable is located at the cell staining position, controlling a blending component on a cell counter to sequentially stain and blend cell samples to be detected in all detection channels of the cell consumable to obtain the stained and blended cell samples in all detection channels; sequentially controlling a plurality of light sources to irradiate the cell samples which are dyed and uniformly mixed in each detection channel, and acquiring target cell images of the cell samples in each detection channel; and carrying out image analysis processing on the target cell images of the cell samples in the detection channels to obtain cell counting results of the cell samples in the detection channels.
It will be understood by those skilled in the art that all or part of the processes of the methods of the embodiments described above can be implemented by hardware instructions of a computer program, which can be stored in a non-volatile computer-readable storage medium, and when executed, can include the processes of the embodiments of the methods described above. Any reference to memory, storage, database or other medium used in the embodiments provided herein can include at least one of non-volatile and volatile memory. Non-volatile Memory may include Read-Only Memory (ROM), magnetic tape, floppy disk, flash Memory, optical Memory, or the like. Volatile Memory can include Random Access Memory (RAM) or external cache Memory. By way of illustration and not limitation, RAM can take many forms, such as Static Random Access Memory (SRAM) or Dynamic Random Access Memory (DRAM), among others.
The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present application, and the description thereof is specific and detailed, but not to be understood as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, and these are all within the scope of protection of the present application. Therefore, the protection scope of the present patent application shall be subject to the appended claims.
Claims (12)
1. A method of cell counting, the method being applied to a cytometer, the method comprising:
acquiring the type of the consumable of the cell consumable to be detected currently;
determining to move the cell consumable to a target position corresponding to the consumable type of the cell consumable according to the consumable type of the cell consumable;
counting cells on the cell consumable at the target location;
the cell counting instrument comprises a light source and at least one photodiode, and an illumination signal of the light source is received by the photodiode after passing through the cell consumable; the method for acquiring the type of the consumable of the cell consumable to be detected currently comprises the following steps:
acquiring the intensity of an illumination signal received by the photodiode;
and determining the consumable type of the cell consumable according to the intensity of the illumination signal.
2. The method of claim 1, wherein the light source of the cytometer is plural, and the obtaining the intensity of the illumination signal received by the photodiode comprises:
and controlling the plurality of light sources to sequentially irradiate the cell consumable to obtain a plurality of illumination signal intensities received by the photodiode.
3. The method according to claim 2, wherein determining the consumable type of the cell consumable from the illumination signal intensity comprises:
determining the arrangement sequence of the intensities of the plurality of illumination signals according to the light-emitting sequence of the plurality of light sources to form an illumination intensity combination;
determining the consumable type of the cell consumable according to the illumination intensity combination and the first corresponding relation; wherein the first correspondence comprises a correspondence between different combinations of illumination intensities and consumable types of different cell consumables.
4. The method according to any one of claims 1 to 3, characterized in that, before said acquiring the type of consumable of the cellular consumable currently to be tested, it further comprises:
sending a starting instruction to a light source to control the light source to emit light;
and determining whether the cell count instrument has the cell consumable to be detected according to the intensity of the illumination signal received by the photodiode.
5. The method of claim 4, further comprising:
if the cell counting instrument is determined to have the cell consumable to be detected, acquiring an identifier of the cell consumable, and sending a consumable data request to a server, wherein the identifier is carried in the consumable data request;
and receiving consumable data which is sent by the server and corresponds to the identification, and determining whether the cell consumable is used according to the consumable data.
6. The method of claim 5, wherein the consumable type of the cellular consumable comprises a manual consumable or an automatic consumable.
7. The method according to claim 2, wherein the determining to move the cell consumable to the target location according to the consumable type of the cell consumable comprises:
if the cell consumable is a manual consumable, moving the cell consumable to a cell counting position; wherein the cell sample in the manual consumable is a stained cell sample;
if the cell consumable is an automatic consumable, moving the cell consumable to a cell staining position; wherein the cell sample in the automated consumable is an unstained cell sample.
8. The method of claim 7, wherein counting cells on the cell consumable at the target location comprises:
if the cell consumable is located at the cell counting position, the light sources are sequentially controlled to irradiate cell samples to be detected in the detection channels of the cell consumable;
when the light source irradiates the cell sample to be detected in the detection channel, controlling a focusing assembly on the cell counter to focus to a target focal length;
acquiring a target cell image of the cell sample in the detection channel under the irradiation of the light source based on the target focal length;
and carrying out image analysis processing on the target cell image to obtain a cell counting result.
9. The method of claim 7, wherein counting cells on the cell consumable at the target location comprises:
if the cell consumable is located at the cell dyeing position, controlling a blending component on the cell counter to sequentially dye and blend cell samples to be tested in each detection channel of the cell consumable to obtain the cell samples dyed and blended in each detection channel;
sequentially controlling the plurality of light sources to irradiate the cell samples which are dyed and uniformly mixed in each detection channel, and acquiring target cell images of the cell samples in each detection channel;
and carrying out image analysis processing on the target cell image of the cell sample in each detection channel to obtain a cell counting result of the cell sample in each detection channel.
10. A cell counting device, which is applied to a cell counter, the device comprising:
the acquisition module is used for acquiring the consumable type of the cell consumable to be detected currently; wherein the consumable type of the cell consumable comprises a manual consumable or an automatic consumable;
the first determination module is used for determining to move the cell consumables to a target position according to the consumable type of the cell consumables;
a counting module for counting the cells on the cell consumable at the target location;
the cell counting instrument comprises a light source and at least one photodiode, and an illumination signal of the light source is received by the photodiode after passing through the cell consumable; the acquisition module comprises an acquisition unit and a determination unit, wherein the acquisition unit is used for acquiring the intensity of the illumination signal received by the photodiode; the determining unit is used for determining the consumable type of the cell consumable according to the intensity of the illumination signal.
11. A computer device comprising a memory and a processor, the memory storing a computer program, characterized in that the processor realizes the steps of the method of any one of claims 1 to 9 when executing the computer program.
12. A computer-readable storage medium, on which a computer program is stored which, when being executed by a processor, carries out the steps of the method according to any one of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111236635.8A CN114067315B (en) | 2021-10-23 | 2021-10-23 | Cell counting method, cell counting device, computer device, and storage medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111236635.8A CN114067315B (en) | 2021-10-23 | 2021-10-23 | Cell counting method, cell counting device, computer device, and storage medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114067315A CN114067315A (en) | 2022-02-18 |
| CN114067315B true CN114067315B (en) | 2022-11-29 |
Family
ID=80235293
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111236635.8A Active CN114067315B (en) | 2021-10-23 | 2021-10-23 | Cell counting method, cell counting device, computer device, and storage medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114067315B (en) |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1675551A (en) * | 2002-08-07 | 2005-09-28 | 塞弗罗有限公司 | Method and device for identifying micro organisms |
| CN102203283A (en) * | 2008-07-21 | 2011-09-28 | 新诊断学股份有限公司 | Methods for the cytological analysis of cervical cells |
| CN102576406A (en) * | 2009-08-31 | 2012-07-11 | 生物辐射实验室股份有限公司 | Compact automated cell counter |
| CN103146800A (en) * | 2013-03-27 | 2013-06-12 | 朱耀辉 | Full-automatic cell non-staining image recognizing and counting method |
| CN103597349A (en) * | 2011-04-07 | 2014-02-19 | 贝克曼考尔特公司 | Identifying and enumerating early granulated cells (EGCs) |
| CN204058472U (en) * | 2014-03-21 | 2014-12-31 | 姬广聚 | A kind of full-automatic cell counter |
| WO2016054293A1 (en) * | 2014-09-30 | 2016-04-07 | The Regents Of The University Of California | Imaging flow cytometer using spatial-temporal transformation |
| CN109269964A (en) * | 2018-10-15 | 2019-01-25 | 深圳博大博聚科技有限公司 | A kind of artificial counting and calibration equipment based on image recognition method cell counter |
| CN109313017A (en) * | 2015-12-18 | 2019-02-05 | 雅培实验室 | The spectral discrimination of histological stain agent |
| CN109900917A (en) * | 2019-04-04 | 2019-06-18 | 电子科技大学 | A kind of portable flow cytometry number system based on micro-fluidic chip |
| JP2019135939A (en) * | 2018-02-07 | 2019-08-22 | 澁谷工業株式会社 | Cell count method, cell count device and cell culture method |
| CN111868260A (en) * | 2017-08-07 | 2020-10-30 | 约翰斯霍普金斯大学 | Methods and materials for assessing and treating cancer |
| WO2020229437A1 (en) * | 2019-05-14 | 2020-11-19 | F. Hoffmann-La Roche Ag | Devices and methods for sample analysis |
| CN112540417A (en) * | 2020-12-28 | 2021-03-23 | 武汉友芝友医疗科技股份有限公司 | Device and method for detecting existence of consumable materials in cell sorter |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6784981B1 (en) * | 2000-06-02 | 2004-08-31 | Idexx Laboratories, Inc. | Flow cytometry-based hematology system |
| US6594009B2 (en) * | 2001-02-27 | 2003-07-15 | Honeywell International Inc. | Flow cytometer and ultraviolet light disinfecting systems |
| CN101622522A (en) * | 2007-01-26 | 2010-01-06 | 贝克顿·迪金森公司 | The method, system and the composition that are used for cell count and analysis |
| CN107169556A (en) * | 2017-05-15 | 2017-09-15 | 电子科技大学 | stem cell automatic counting method based on deep learning |
| JP7045046B2 (en) * | 2017-07-10 | 2022-03-31 | 国立大学法人東海国立大学機構 | Cyanine compound and fluorescent dye using it |
| CN108982334A (en) * | 2018-06-05 | 2018-12-11 | 江苏卓微生物科技有限公司 | Cell counter and its application |
| CN109100286A (en) * | 2018-10-31 | 2018-12-28 | 江苏卓微生物科技有限公司 | cell counter |
| CN110554491B (en) * | 2018-12-12 | 2022-04-22 | 西安理工大学 | Miniature phase difference digital microscope for observing unstained cells of living body |
| CN110706206A (en) * | 2019-09-11 | 2020-01-17 | 深圳先进技术研究院 | Fluorescent cell counting method, fluorescent cell counting device, terminal equipment and storage medium |
| JP2021083431A (en) * | 2019-11-29 | 2021-06-03 | シスメックス株式会社 | Cell analysis method, cell analysis device, cell analysis system and cell analysis program |
| EP4071485A4 (en) * | 2019-12-06 | 2023-04-12 | Shenzhen Mindray Bio-Medical Electronics Co., Ltd | Sample analysis system and method, cell image analyzer, and storage medium |
| CN111088313B (en) * | 2019-12-16 | 2024-05-14 | 中国水产科学研究院珠江水产研究所 | Reagent for rapidly fixing cell count of tetrahymena thermophila and counting method thereof |
| CN111973334A (en) * | 2020-07-29 | 2020-11-24 | 烯旺新材料科技股份有限公司 | New application of graphene in immune enhancement |
| CN112435259B (en) * | 2021-01-27 | 2021-04-02 | 核工业四一六医院 | Cell distribution model construction and cell counting method based on single sample learning |
| CN113515077A (en) * | 2021-04-23 | 2021-10-19 | 重庆德方信息技术有限公司 | System and method for monitoring human body cell staining process |
-
2021
- 2021-10-23 CN CN202111236635.8A patent/CN114067315B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1675551A (en) * | 2002-08-07 | 2005-09-28 | 塞弗罗有限公司 | Method and device for identifying micro organisms |
| CN102203283A (en) * | 2008-07-21 | 2011-09-28 | 新诊断学股份有限公司 | Methods for the cytological analysis of cervical cells |
| CN102576406A (en) * | 2009-08-31 | 2012-07-11 | 生物辐射实验室股份有限公司 | Compact automated cell counter |
| CN103597349A (en) * | 2011-04-07 | 2014-02-19 | 贝克曼考尔特公司 | Identifying and enumerating early granulated cells (EGCs) |
| CN103146800A (en) * | 2013-03-27 | 2013-06-12 | 朱耀辉 | Full-automatic cell non-staining image recognizing and counting method |
| CN204058472U (en) * | 2014-03-21 | 2014-12-31 | 姬广聚 | A kind of full-automatic cell counter |
| WO2016054293A1 (en) * | 2014-09-30 | 2016-04-07 | The Regents Of The University Of California | Imaging flow cytometer using spatial-temporal transformation |
| CN109313017A (en) * | 2015-12-18 | 2019-02-05 | 雅培实验室 | The spectral discrimination of histological stain agent |
| CN111868260A (en) * | 2017-08-07 | 2020-10-30 | 约翰斯霍普金斯大学 | Methods and materials for assessing and treating cancer |
| JP2019135939A (en) * | 2018-02-07 | 2019-08-22 | 澁谷工業株式会社 | Cell count method, cell count device and cell culture method |
| CN109269964A (en) * | 2018-10-15 | 2019-01-25 | 深圳博大博聚科技有限公司 | A kind of artificial counting and calibration equipment based on image recognition method cell counter |
| CN109900917A (en) * | 2019-04-04 | 2019-06-18 | 电子科技大学 | A kind of portable flow cytometry number system based on micro-fluidic chip |
| WO2020229437A1 (en) * | 2019-05-14 | 2020-11-19 | F. Hoffmann-La Roche Ag | Devices and methods for sample analysis |
| CN112540417A (en) * | 2020-12-28 | 2021-03-23 | 武汉友芝友医疗科技股份有限公司 | Device and method for detecting existence of consumable materials in cell sorter |
Non-Patent Citations (6)
| Title |
|---|
| "Automatic Cell Counter for cell viability estimation";Lojk J等;《International Convention on Information and Communication Technology，Electronics and Microeletronics》;20141231;第239-244页 * |
| "Cell Counter：ImageJ Plugin to automatic count of cells from unique and Multiple color image";Pierre Decazes;《ResearchGate》;20180331;第345-349页 * |
| "一种pH响应型光敏剂的活性和细胞毒性";黄晓晨等;《三明学院学报》;20190831;第36卷(第4期);第7-12页 * |
| "单细胞分析技术在肿瘤研究中的应用进展";宋琦等;《实用医学杂志》;20191231;第35卷(第2期);第322-324页 * |
| "国产细胞计数仪怎么选？";_Danabas;《https：//www.sohu.com/a/286165171_99982681》;20190103;第7页 * |
| "水溶性聚噻吩衍生物的合成、表征及细胞成像";王凤燕等;《高等学校化学学报》;20181130;第39卷(第11期);第2594-2600页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114067315A (en) | 2022-02-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7526116B2 (en) | Automated microscopic sperm identification | |
| US9338408B2 (en) | Image obtaining apparatus, image obtaining method, and image obtaining program | |
| US20070114388A1 (en) | Imaging mass spectrometer | |
| JP5407015B2 (en) | Image processing apparatus, image processing method, computer-executable image processing program, and microscope system | |
| US20130141561A1 (en) | Method of analyzing linearity of shot image, image obtaining method, and image obtaining apparatus | |
| US9438848B2 (en) | Image obtaining apparatus, image obtaining method, and image obtaining program | |
| JP2000180922A (en) | Photographing system | |
| CN109852540B (en) | Image analysis device and image analysis method | |
| JP2000180361A (en) | Photographing system | |
| US10591402B2 (en) | Image processing apparatus, image processing method, and image processing program | |
| CN109643453A (en) | System and method for the image analysis based on template | |
| CN114829933A (en) | Sample analysis system and automatic precision management method thereof | |
| CN114067315B (en) | Cell counting method, cell counting device, computer device, and storage medium | |
| JPWO2017018047A1 (en) | Specimen staining system | |
| JP5953195B2 (en) | Imaging analysis apparatus, control method thereof, and program for imaging analysis apparatus | |
| CN112884751B (en) | Device and method for synchronously measuring deformation and temperature by using ultraviolet fluorescent mark | |
| JP2000235003A (en) | Photographing method and photographing device | |
| CN114066818B (en) | Cell detection analysis method, cell detection analysis device, computer equipment and storage medium | |
| CN112858702A (en) | Sample detection-based information processing method, sample detection system and storage medium | |
| CN110993062B (en) | Medical image information system | |
| KR102602005B1 (en) | charged particle beam device | |
| CN100481095C (en) | System and method for fully automated robotic-assisted image analysis for in vitro and in vivo genotoxicity testing | |
| CN111326237A (en) | Reordering processing method and device, electronic equipment and storage medium | |
| US8963105B2 (en) | Image obtaining apparatus, image obtaining method, and image obtaining program | |
| CN114088606B (en) | Cell analysis device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |