CN204058472U - A kind of full-automatic cell counter - Google Patents
A kind of full-automatic cell counter Download PDFInfo
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- CN204058472U CN204058472U CN201420129546.2U CN201420129546U CN204058472U CN 204058472 U CN204058472 U CN 204058472U CN 201420129546 U CN201420129546 U CN 201420129546U CN 204058472 U CN204058472 U CN 204058472U
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Abstract
The utility model relates to a kind of cell counter, especially relates to a kind of full-automatic cell counter.Comprise pointolite, be arranged on convex lens below pointolite, be arranged on below convex lens for place cell suspension to be measured count slice, be arranged on focusing object lens below count slice, be arranged on the spectral filter below focusing object lens and be arranged on be converted to electrical signal for the optical signal obtained by sensitive chip below spectral filter after be used for the analytical system analyzed.Therefore, the utility model tool has the following advantages: can automatically identify non-staining cell, also automatically can identify staining cell; The utility model uses the method for pattern recognition, avoids the shortcoming that the accuracy of electric impedance counting process is low, range of application is narrow, needs often correct; Cell through the utility model counting can be cultivated (amplification) again, ensure that the continuity of scientific research.
Description
Technical field
The utility model relates to a kind of cell counter, especially relates to a kind of full-automatic cell counter.
Background technology
Because cell must keep certain density just can have good activity when cultivating, therefore all need repeatedly to carry out cell counting according to practical situation in the recovery of cell, cultivation, frozen process; Need different cell concns in cell therapy clinical application, therefore need cell counting.
Method conventional at present comprises pattern-recognition method and electric impedance counting process.
(1) pattern-recognition method principle is: by cell suspension after the dyeing of placenta indigo plant, add the blood cell counting plate of glass, amplified by cell image through micro-amplification light path, and viable cell central zone is bright around has a circle blue, is rendered as one bright circular; The image of dead cell is blue spot (can differentiate with background color).During artificial counting, need operator to count the sum of viable cell and dead cell under the microscope one by one, extrapolate the concentration of dead cell and viable cell; During Auto-counting, the special computer picture recognition software of instrument application is captured by figure, the parameter such as circularity, diameter, color gray scale of analysis image, automatically can calculate viable cell concentrations in tally (quantity/milliliter), dead cell concentration (quantity/milliliter), total cellular score (containing dead cell and viable cell) value, the data such as live cell fraction, and can be stored in instrument or transfer to other computer-readable storage mediums by USB flash disk etc.
(2) shortcoming of current pattern-recognition method:
1, conventional in these class methods dyestuff is core dyestuff, has certain toxicity to viable cell;
2, can only realize off-line counting, cell must depart from culture system and can count, and can not realize real-time online counting (not departing from culture system);
3, can not re-use after the value-added cell counting of follow-up cultivation being needed for embryonic stem cell etc.
4, activity and the quantity with endocytosis cell cannot be judged, and be in the cell (now cell has entered irreversible death program, but still refuses to contaminate trypan blue) of apoptosis process middle and later periods.
Based on what time above, there is larger limitation in traditional dyed cell counting carrying out pattern recognition again.
(3) electric impedance counting process: to detect the cell that suspends in electrolyte solution based on the resistance change by causing during counting channel, carry out the mensuration of cell counting and volume, this method is called electrical impedance method, also referred to as Coulter principle.
(4) electrical impedance method shortcoming:
1, because the internal diameter of the aperture pipe in electrical impedance method only has 25 μm, the instrument of electric impedance is therefore adopted all cannot to count the cell (the ovum diameter of the such as mankind reaches 100 μm) that diameter is greater than 25 μm at present.
2, cell debris is formed agglomerate, impurity agglomerate in diluent and Doublet Cell all can affect Cytometric accuracy.
3, the count signal of cell and undesired signal are exaggerated in amplification process simultaneously, have impact on the reliability of result greatly.
4, in order to make instrument accurately can screen cell, appliance requires carries out regular Quality Control, resets and determines threshold value.
5, the existence detecting electric field is to the activity of cell and cultivate and also can produce detrimentally affect.
Utility model content
The utility model mainly solves the technical problem existing for prior art; Provide one and can automatically identify non-staining cell, also automatically can identify staining cell.Overcome a kind of full-automatic cell counter that traditional images method of identification needs the shortcoming of dyeing;
Secondly the utility model is solve the technical problem existing for prior art; Provide a kind of method using pattern recognition, avoid the shortcoming that the accuracy of electric impedance counting process is low, range of application is narrow, needs often correct; Cell through the utility model counting can be cultivated (amplification) again, ensure that successional a kind of full-automatic cell counter of scientific research.
Above-mentioned technical problem of the present utility model is mainly solved by following technical proposals:
A kind of full-automatic cell counter, it is characterized in that, comprise pointolite, be arranged on convex lens below pointolite, be arranged on below convex lens for place cell suspension to be measured count slice, be arranged on focusing object lens below count slice, be arranged on the spectral filter below focusing object lens and be arranged on be converted to electrical signal for the optical signal obtained by spectral filter below spectral filter after be used for the analytical system analyzed.Final realization does not need accurately to judge dead cell or viable cell state and tally function during dyeing (non-staining).
At above-mentioned a kind of full-automatic cell counter, described analytical system comprises and to be positioned at below spectral filter for receiving optical signals and optical signal to be converted to the sensitive chip of electrical signal, a controller, connection control device and the data line of sensitive chip and the indicating meter be connected with controller.
At above-mentioned a kind of full-automatic cell counter, described sensitive chip adopts magnesium light company MT9T001 chip; Described controller is computer.
Therefore, the utility model tool has the following advantages: can automatically identify non-staining cell, also automatically can identify staining cell; The utility model uses the method for pattern recognition, avoids the shortcoming that the accuracy of electric impedance counting process is low, range of application is narrow, needs often correct; Cell through the utility model counting can be cultivated (amplification) again, ensure that the continuity of scientific research.
Accompanying drawing explanation
Accompanying drawing 1 is a kind of structural principle schematic diagram of the present utility model.
Embodiment
Below by embodiment, and by reference to the accompanying drawings, the technical solution of the utility model is described in further detail.
Embodiment:
First, introduce structure the utility model of the present utility model comprise pointolite 1, be arranged on convex lens 2 below pointolite 1, be arranged on below convex lens 2 for place cell suspension to be measured count slice 3, be arranged on focusing object lens 4 below count slice 3, be arranged on the spectral filter 5 below focusing object lens 4 and be arranged on be converted to electrical signal for the optical signal obtained by spectral filter 5 below spectral filter 5 after be used for the analytical system analyzed.
Analytical system comprises and to be positioned at below spectral filter 5 for receiving optical signals and sensitive chip 6, controller 8, the connection control device 8 optical signal being converted to electrical signal and the data line 7 of sensitive chip 6 and the indicating meter 10 that is connected with controller 8.
During use, first get cell suspension 10ul to be measured with pipettor, need not dye, directly to add in 3 count slices and inserting instrument;
Open the optimum configurations of sensitive chip (Fig. 1 the 6th);
First do white balance, all parameter values after record white balance: R(ruddiness gain), the gain of G(green glow), the gain of B(blue light), gamma value, contrast gradient, auto color gain, automatic exposure value etc., using these parameter values as basic value (hereinafter referred to as basic value);
Reduce RG (red green) gain, reduce to basic value 1/3 to 2/3 between.Namely the receiving capability (instrument has been adjusted to optimum value and has been set to default value) of sensitive chip (Fig. 1 the 6th) to RG (red green) light intensity is reduced;
Increase the gain of B (indigo plant), be increased to 1 to 2 times of basic value.Namely the receiving capability (instrument has been adjusted to optimum value and has been set to default value) of sensitive chip (Fig. 1 the 6th) to B (indigo plant) light intensity is enhanced;
Now 10 display background are light blue: the light planoconvex lens (Fig. 1 the 2nd) that pointolite (Fig. 1 the 1st) sends, count slice (Fig. 1 the 3rd), focusing object lens (Fig. 1 the 4th), colour filter (Fig. 1 the 5th), sensitive chip (Fig. 1 the 6th) convert optical signal to electrical signal, and being transferred to controller (Fig. 1 the 8th) through data line (Fig. 1 the 7th), the background finally above shown at indicating meter (Fig. 1 the 10th) is light blue (instrument has been adjusted to optimum value and has been set to default value).
Spectral filter (Fig. 1 the 5th) in instrument light path prevents the interference of RG (red green) light, only allows B (indigo plant) light to pass through, and B (indigo plant) light intensity of at this moment viable cell reflection is greater than B (indigo plant) light intensity of dead cell reflection;
Turn off automatic exposure function, manual regulation time shutter and exposure gain, the sensitive chip (Fig. 1 the 6th) of different sorts and manufacturer production needs the scope that regulates different, and checkmating, cell image and background obviously distinguish be as the criterion (instrument has been adjusted to optimum value and has been set to default value).Concrete control method: B (indigo plant) light of viable cell reflection because of intensity large, first go up imaging at sensitive chip (Fig. 1 the 6th), indicating meter (Fig. 1 the 10th) can see circular locus coeruleus (darker than background color) gradually; Continue to increase the time shutter, the circular locus coeruleus (darker than background color) first occurred becomes circular speck (because the time shutter is long, occurring overexposure phenomenon), has new circular locus coeruleus (darker than background color) simultaneously and occurs.Now, the background of indicating meter (Fig. 1 the 10th) is light blue, and circular speck is the image of viable cell, and circular locus coeruleus (than background blue color depth) is exactly the image of dead cell.By non-staining cell image recognition software analysis (Fig. 1 the 9th), distinguish the quantity, concentration, diameter, area, ratio etc. of dead cell and viable cell.
This utility model instrument can realize the online cell counting of non-staining through transformation.
Here is simultaneous test.
Use pipettor obtained cell suspension 50ul respectively, add A pipe and B pipe; B pipe is added 50ul placenta indigo plant (0.4%) to mix afterwards;
Get from A pipe " 1 " position that 10ul adds count slice, more renew move liquid head after get from B pipe " 2 " position that 10ul adds count slice.
By in count slice inserting instrument.
Use " non-dye " function counting " 1 " position.
Use " dyeing " function counting " 2 " position.
Two kinds of method count results errors, within 5%, by further optimized image identification software, can improve the accuracy of two kinds of methods simultaneously.
In the present embodiment, controller and computer are known technology, be not emphasis, mainly protected the hardware configuration of optical system by utility model in the utility model.
Specific embodiment described herein is only to the explanation for example of the utility model spirit.The utility model person of ordinary skill in the field can make various amendment or supplements or adopt similar mode to substitute to described specific embodiment, but can't depart from spirit of the present utility model or surmount the scope that appended claims defines.
Claims (1)
1. a full-automatic cell counter, it is characterized in that, comprise pointolite, be arranged on convex lens below pointolite, be arranged on below convex lens for place cell suspension to be measured count slice, be arranged on focusing object lens below count slice, be arranged on the spectral filter below focusing object lens and be arranged on be converted to electrical signal for the optical signal obtained by sensitive chip below spectral filter after be used for the analytical system analyzed;
Described analytical system comprises and to be positioned at below spectral filter for receiving optical signals and optical signal to be converted to the sensitive chip of electrical signal, a controller, connection control device and the data line of sensitive chip and the indicating meter be connected with controller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420129546.2U CN204058472U (en) | 2014-03-21 | 2014-03-21 | A kind of full-automatic cell counter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420129546.2U CN204058472U (en) | 2014-03-21 | 2014-03-21 | A kind of full-automatic cell counter |
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| Publication Number | Publication Date |
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| CN204058472U true CN204058472U (en) | 2014-12-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420129546.2U Expired - Fee Related CN204058472U (en) | 2014-03-21 | 2014-03-21 | A kind of full-automatic cell counter |
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| Country | Link |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021114086A1 (en) * | 2019-12-10 | 2021-06-17 | 苏州生动细胞生物科技有限公司 | Cell counting device |
| CN114067315A (en) * | 2021-10-23 | 2022-02-18 | 广州市艾贝泰生物科技有限公司 | Cell counting method, cell counting device, computer device, and storage medium |
-
2014
- 2014-03-21 CN CN201420129546.2U patent/CN204058472U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021114086A1 (en) * | 2019-12-10 | 2021-06-17 | 苏州生动细胞生物科技有限公司 | Cell counting device |
| CN114067315A (en) * | 2021-10-23 | 2022-02-18 | 广州市艾贝泰生物科技有限公司 | Cell counting method, cell counting device, computer device, and storage medium |
| CN114067315B (en) * | 2021-10-23 | 2022-11-29 | 广州市艾贝泰生物科技有限公司 | Cell counting method, cell counting device, computer device, and storage medium |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Shenzhen Boda Technology Co., Ltd. Assignor: Yuan Zengqiang|Ji Lin|Lin Wei|Zhu Yaohui Contract record no.: 2015990000130 Denomination of utility model: Full-automatic cell counter Granted publication date: 20141231 License type: Exclusive License Record date: 20150325 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141231 Termination date: 20190321 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |