JP2000235003A - Photographing method and photographing device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To simplify the switching setting operation of a photographing device according to the kind of emitted light (photographing style) to be detected, in the case of a photographing device for which setting items are prepared according to a plurality of photographing styles. SOLUTION: In this photographing device, a look-up table in which the kinds of detecting light to be detected are associated with combinations of the kinds of irradiation light (a while LED light source 45a, a blue LED light source 45b, and a fluorescent lamp 45c), diaphragm 41 (an open diaphragm 41a, a first diaphragm 41b, and a second diaphragm 41c), and a filter (whether an excitation-light cutting filter 42 is used or not) of a photographing device body 20, is previously stored in a memory 71a of a computer body 71, and a changeover means 60 obtains a combination corresponding to the kind of detection light (photographing style) inputted into a keyboard 73 by referring to the look-up table and changes over the kinds of illumination light, etc., so as to satisfy the combination.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a photographing system and, more particularly, to an improvement of a photographing method and a photographing apparatus in which a plurality of photographing methods are prepared so as to be switchable depending on a photographing object.

[0002]

2. Description of the Related Art Conventionally, in the field of biochemistry and molecular biology, as a method of detecting nucleic acids and proteins in a membrane filter or the like after blotting, a chemical reaction that chemically reacts with a specific protein or the like to emit light (chemiluminescence). A chemiluminescence method is known in which a substance is labeled on the protein and an image of the chemiluminescence is captured using a photoelectric reading means such as a CCD. A chemiluminescence method is known as an imaging device for capturing such chemiluminescence imaging. A filter or the like is placed on a suitable sample table and housed in a dark box shielded from external light, and in this dark box, chemiluminescent light emitted from a membrane filter or the like is photoelectrically scanned by a photoelectric reading means via a lens. To obtain image information indicating the distribution of specific proteins and the like that react with a predetermined luminescent chemical substance It is known.

[0003] On the other hand, in the same field, a fluorescence detection (fluoresceuce) system using a fluorescent dye as a labeling substance is known. This system irradiates a sample in which a specific biological substance labeled with a fluorescent dye is distributed with excitation light, and captures an image of the fluorescence excited by the irradiation of the excitation light using a photoelectric reading unit, It reads image information indicating the distribution state of fluorescent dyes, that is, the distribution state of a specific biological substance, the gene sequence, gene expression level, the metabolism, absorption, excretion pathway and state of the administered substance in experimental mice, protein Separation, identification, or evaluation of molecular weight and characteristics.

Here, the above-described photographing apparatus for detecting chemiluminescence is provided with an excitation light source that emits excitation light capable of exciting a fluorescent dye, and a method in which only fluorescence enters the photoelectric reading unit and the excitation light enters. By further providing an excitation light cut filter that blocks the light and a predetermined stop, the device can function as an imaging device for the above-described fluorescence detection system, and such an imaging device has already been developed.

That is, when performing imaging for detecting chemiluminescence, the excitation light is prevented from being emitted, the excitation light cut filter is retracted from the optical path of the chemiluminescence, and the aperture is opened to be emitted from the sample. When the chemiluminescent light is detected by the photoelectric reading means, and when imaging for fluorescence detection is performed, the excitation light is emitted to irradiate the sample, and the excitation light cut filter is irradiated with the fluorescent light from the sample to the photoelectric reading means. In order to ensure only the fluorescence by the photoelectric reading means, the aperture may be switched from the open state in order to secure the depth of field corresponding to the thickness of the sample.

Further, this photographing device irradiates a transparent original such as a film or a reflective original such as a photograph with white light and photoelectrically reads the transmitted light or the reflected light by a photoelectric reading means via a lens to obtain a digital image. Can also be used as a digitizer to obtain In this case, an illumination light source that emits white light is provided separately from the excitation light source that is provided for fluorescence detection, and a diaphragm that further narrows the amount of light incident on the photoelectric reading unit is provided than a diaphragm that is used for fluorescence detection. I just need.

[0007]

By the way, the above-mentioned three types of photographing methods (chemiluminescence detection, fluorescence detection and digitizing) (type of irradiation light, degree of aperture, type of filter used) can be switched and set. In the above, the operator must perform an operation of switching the irradiation light, the aperture, and the filter in accordance with the type of the emitted light to be detected. However, an operator skilled in such an operation can immediately associate the type of emitted light to be detected with the imaging method, and can quickly and accurately perform the above-described switching setting operation. Although it is possible, an unskilled operator may take a long time to perform the switching setting operation or may make an incorrect setting.

[0008] Even for a skilled operator, the operation of switching the irradiation light, the aperture, and the filter one by one is troublesome, and the possibility of a setting error cannot be denied.

SUMMARY OF THE INVENTION The present invention has been made in view of the above circumstances, and has as its object to provide a photographing method and a photographing apparatus in which the switching setting operation of the photographing apparatus is simplified according to the type of emitted light to be detected. It is assumed that.

[0010]

According to the photographing method of the present invention, the emitted light to be detected is associated with a photographing method in advance, and the type of the emitted light to be detected is input.
The photographing method is automatically switched in accordance with the photographing method associated with the emitted light.

That is, according to the imaging method of the present invention, the emitted light emitted from the sample is photoelectrically detected by the photoelectric reading means.
In an imaging method in which the type of light irradiating the sample, the degree of a diaphragm arranged between the sample and the photoelectric reading unit, and the type of filter can be switched according to the type of the emitted light, According to the type of the emitted light, a combination of the type of the light to be irradiated, the degree of the aperture to be arranged, and the type of the filter to be arranged is stored in a reference table in advance, and the emitted light to be detected is Receiving the input of the selection of the type, the type of light to be irradiated according to the type of the selected emission light,
The combination of the degree of the aperture to be arranged and the type of the filter to be arranged is determined by referring to the reference table, and according to the determined combination, the type of light to be irradiated, the degree of the aperture to be arranged, and the arrangement This is characterized in that the types of filters to be switched are switched.

Here, the reference table is a combination of, for example, when the emitted light to be detected is chemiluminescent light, there is no light to be irradiated, the aperture to be arranged is in an open state, and no filter is arranged. When the emitted light to be emitted is fluorescent light, the emitted light is excitation light for exciting the fluorescence, the aperture to be arranged is a first aperture state narrower than the open state, the filter to be arranged cuts the excitation light, and When the emitted light to be detected is reflected light or transmitted light from the sample, the light to be irradiated is white light, and the stop to be arranged is the above-described aperture. The second aperture state, which is further narrowed than the first aperture state, may be a combination without any filter.

Further, when the emitted light to be detected is fluorescent, the light to be detected is further subdivided, and when the emitted light to be detected is fluorescent by the fluorescent reagent FITC (fluorescein isothiosilanate), The correspondence may be different depending on the case where the light is fluorescent with the fluorescent reagent Cy5 (Sci Five; trade name of Amersham Pharmacia Biotech Co., Ltd.). That is, specifically, in the case of the fluorescence by the fluorescent reagent FITC, the irradiation light is excitation light (specifically, blue light) for exciting the fluorescence by the FITC, the diaphragm to be arranged is the first diaphragm state, and the filter to be arranged. Is a combination of an excitation light cut filter (specifically, a yellow transmission filter) whose band is set so as to cut the excitation light and transmit the fluorescence by the FITC, and the emitted light to be detected is the fluorescence of the fluorescent reagent Cy5. At this time, the irradiation light is excitation light (specifically, red light) that excites the fluorescence by Cy5, the aperture to be arranged is the first aperture state, the filter to be arranged cuts the excitation light, and the fluorescence by Cy5 is emitted. What is necessary is just to make a reference table a correspondence as a combination of an excitation light cut filter (specifically, a near-infrared light transmission filter) whose band is set to be transmitted.

When the emitted light to be detected is transmitted light or reflected light, the light may be further divided and the correspondence between transmitted light and reflected light may be divided. That is, specifically, in the case of transmitted light, the light to be irradiated is white light that irradiates the sample from a direction in which the transmitted light of the sample (transmitted original) is read photoelectrically, the aperture to be arranged is the second aperture state, In the case of reflected light, the light to be irradiated is white light for irradiating the sample from the direction in which the reflected light of the sample (reflecting document) is read photoelectrically, and the diaphragm to be disposed is the second light. What is necessary is just to make a reference table a correspondence in which the combination of the aperture state and the filter to be arranged is absent.

The photoelectric reading means has a wide dynamic range capable of detecting weak chemiluminescence and fluorescence,
In addition, it is preferable to use an interline type CCD having a cooling element, which can detect with good linearity and can also take out a pseudo moving image by repeating photoelectric reading for a short time.

An imaging apparatus according to the present invention is an apparatus for carrying out the imaging method according to the present invention, wherein emitted light emitted from a sample is photoelectrically detected by photoelectric reading means. In an imaging device in which the type of light to be irradiated on the sample, the degree of the diaphragm arranged between the sample and the photoelectric reading means, and the type of filter can be switched, the type of light emitted depends on the type of the emitted light to be detected. In addition, a storage unit in which a combination of the type of light to be irradiated, the degree of the aperture to be arranged, and the type of the filter to be arranged is stored in advance as a reference table, and selection of the type of the emitted light to be detected is input. Referring to the reference table, the type of the light to be irradiated corresponding to the type of the emitted light input to the input method, Switching means for determining the combination of the degree of the aperture to be arranged and the type of the filter to be arranged, and switching the type of the light to be irradiated, the degree of the aperture to be arranged, and the type of the filter to be arranged according to the determined combination. It is characterized by having.

Here, as the reference table stored in the storage means, for example, when the emitted light to be detected is chemiluminescent light, the light to be irradiated is similar to that described in the photographing method of the present invention. None, the aperture to be arranged is in an open state, and the filter to be arranged is a combination of no.When the emitted light to be detected is fluorescent, the light to be irradiated is excitation light for exciting fluorescence, and the aperture to be arranged is more than in the open state. The first aperture state that is narrowed down, the filter to be arranged cuts the excitation light,
It is a combination of an excitation light cut filter whose band is set so as to transmit fluorescence, and when the emission light to be detected is reflected light or transmitted light from the sample, the light to be irradiated is white light, and the aperture to be arranged is What is necessary is just to set it as the combination of the 2nd aperture state narrowed further than the said 1st aperture state, and no filter to arrange.

Further, when the emitted light to be detected is fluorescent, the light is further subdivided, and when the emitted light to be detected is fluorescent by the fluorescent reagent FITC, and when the emitted light to be detected is fluorescent by the fluorescent reagent Cy5. The correspondence may be divided between the case of fluorescence and the case of fluorescence. That is, specifically, in the case of the fluorescence by the fluorescent reagent FITC, the irradiation light is excitation light (specifically, blue light) for exciting the fluorescence by the FITC, the diaphragm to be arranged is the first diaphragm state, and the filter to be arranged. Is a combination of an excitation light cut filter (specifically, a yellow transmission filter) whose band is set so as to cut the excitation light and transmit the fluorescence by the FITC, and the emitted light to be detected is the fluorescence of the fluorescent reagent Cy5. At this time, the irradiation light is excitation light (specifically, red light) that excites the fluorescence by Cy5, the aperture to be arranged is the first aperture state, the filter to be arranged cuts the excitation light, and the fluorescence by Cy5 is emitted. What is necessary is just to make a reference table a correspondence as a combination of an excitation light cut filter (specifically, a near-infrared light transmission filter) whose band is set to be transmitted. If the emitted light to be detected is transmitted light or reflected light, the light may be further subdivided and the correspondence between transmitted light and reflected light may be divided. That is, specifically, in the case of transmitted light, the light to be irradiated is white light that irradiates the sample from a direction in which the transmitted light of the sample (transmitted original) is read photoelectrically, the aperture to be arranged is the second aperture state, In the case of reflected light, the light to be irradiated is white light for irradiating the sample from the direction in which the reflected light of the sample (reflecting document) is read photoelectrically, and the diaphragm to be disposed is the second light. What is necessary is just to make the reference table a combination in which the combination of the aperture state and the filter to be arranged is absent.

The photoelectric reading means has a wide dynamic range capable of detecting weak chemiluminescence and fluorescence.
In addition, it is preferable to use an interline type CCD having a cooling element, which can detect with good linearity and can also take out a pseudo moving image by repeating photoelectric reading for a short time.

The storage means and the photographing method input means are not limited to those which are physically integrated with the photographing apparatus main body such as the photoelectric reading means and the photographing quality (dark box) in which the sample is arranged. A personal computer or the like systemized with the main body of the photographing apparatus for performing image processing, analysis processing, and the like on image information obtained by the photographing apparatus, and these storage means and photographing method input means are provided. Alternatively, it may of course be configured integrally with the imaging device body.

[0021]

According to the photographing method and photographing apparatus of the present invention,
The type of emitted light that is to be photoelectrically detected by the photoelectric reading unit and the imaging method (the type of light applied to the sample, the degree of the diaphragm disposed between the sample and the photoelectric reading unit, and the type of filter) The type of the emitted light to be detected or the name of the imaging method (or a number or the like associated with the name) is input to the imaging method input means in advance by associating it with the reference table. The photographing method associated with the light is obtained by referring to the reference table, and the switching unit automatically switches to the obtained photographing method, and according to the type of light to be detected,
The switching setting operation of the photographing device can be simplified.

Therefore, there is no need for the operator to switch the irradiation light, the aperture, and the filter of the photographing apparatus, and the above-described switching setting operation can be performed quickly and accurately regardless of the skill level of the operator.

[0023]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, a specific embodiment of a photographing apparatus for carrying out the photographing method of the present invention will be described with reference to the drawings.

FIG. 2 is a perspective view showing a schematic configuration of an embodiment of the photographing apparatus according to the present invention, and FIG. 1 is a view showing the entire structure including the inside of the photographing apparatus shown in FIG. Illustrated photographing device 10
Reference numeral 0 denotes a photographing apparatus main body 20 including a CCD 51 for photographing a sample, and a personal computer 70 for performing image processing or the like on an image signal obtained by photographing.

The photographing apparatus main body 20 is provided with a lens 40 movable in the optical axis X direction and a tray 10 on which a predetermined sample 11 is placed. , 21g formed at seven discrete positions having different distances from each other, and one tray rail on which the tray 10 is actually arranged among the seven tray rails is detected. A tray rail detection sensor 22a,..., 22g, an interline CCD 51 having a cooling element for photoelectrically reading a distribution image of emission light emitted from the sample 11 formed on the light receiving surface by the lens 40, Tray rail detection sensors 22a,.
Light emitted from the sample 11 on the light receiving surface of the CCD 51 according to the rail (one of 21a,..., 21g on which the tray 10 is actually mounted, 21d in the drawing) detected by 22g. Lens moving means 32 for moving the lens 40 in the optical axis X direction so that a light image is formed;
And the sample 11 when the sample 11 is a fluorescence detection sample, provided between the lens 40 and the CCD 51 so as to be able to enter and exit on an optical path of light constituting an image of the sample 11.
The excitation light cut filter 42 is provided with a band set so as to transmit the fluorescent light emitted from the filter and block the transmission of the blue LED light for exciting the fluorescent light, and is provided between the lens 40 and the excitation light cut filter 42. The first stop 41b and the first stop 4 whose apertures are in the open state, the aperture amount of which is smaller than that of the open stop 41a;
A second aperture stop 4 that further reduces the amount of light passing therethrough than 1b
A variable diaphragm 41 for selectively arranging 1c on the optical path;
White LED light source 45a that emits white LED light, blue L
The excitation is performed by referring to a light source including a blue LED light source 45b that emits ED light and a fluorescent lamp 45c that emits white light from below the sample 11, and a lookup table stored in a memory 71a of a personal computer 70 described later. A configuration including switching means 60 for switching the state of the light cut filter 42 into and out of the optical path, the aperture 41 and the light source, a camera controller 52 for controlling the exposure of the CCD 51, and a dark box 25 as a case of the photographing apparatus main body 20. It is.

Here, the tray rail detection sensor 22a,
, 22g are tray rails 21a, ..., 21g, respectively.
It corresponds to.

The personal computer 70 mainly performs image processing and the like on image signals obtained by photographing. The personal computer 70 comprises a keyboard 73, a mouse 74, a CRT 72 and a computer main body 71 which are man / machine interfaces. As shown in Table 1 below, the memory 71a stores a look-up table in which detection light that is an input, the type of irradiation light that is an output, and a combination of an aperture and a filter are associated in advance.

[0028]

[Table 1]

The computer main body 71 includes a keyboard 73
Alternatively, an exposure start signal is transmitted to the camera controller 52 in accordance with an exposure start signal input from the mouse 74, and an image signal photoelectrically read by the CCD 51 after the exposure is completed is transmitted to the camera controller 5.
2 and performs signal processing such as image processing.

Next, the operation of the photographing apparatus 100 of the present embodiment will be described.

First, an operator places a sample (for example, a membrane filter on which a substance exhibiting chemiluminescence is distributed) 11 for detecting chemiluminescence at a predetermined position on the tray 10.
A tray rail suitable for photographing at a desired angle of view is selected (in this embodiment, tray rail 21d).
Then, the tray 10 is arranged on the tray rail 21d. Here, the lens 40 is initially stopped at a predetermined initial position.

When the tray 10 is placed by the operator, the sensor 22d corresponding to the fourth rail 21d on which the tray 10 is placed is placed on the tray 10 among the sensors 22a to 22g placed in the dark box 25. Is detected, and this detection signal is sent to the lens moving means 32. At this time, the other sensors 22a to 22c and 22e to 22e
g are the corresponding rails 21a to 21c and 2 respectively.
Since the tray 10 does not exist in 1e to 21g, no detection signal is output.

The lens moving means 32 receives the detection signal, recognizes that the detection signal has been output from the sensor 22d, and operates the fourth rail 2 corresponding to the sensor 22d.
The image of the sample 11 on the tray 10 placed on the
The lens 40 is moved in the optical axis X direction to a position suitable for forming an image on the light receiving surface of D51 without defocus.

Next, the operator opens and closes the door 25 of the dark box 25.
a to close the dark box 25, and
Then, an input to the effect that the photographing target is the chemiluminescent light is made on the keyboard 73 of No. 0. The input items here may be any specific input contents as long as they correspond to the chemiluminescent light on a one-to-one basis, and may be, for example, a “chemoluminescent light imaging method”. Good. The input method may be a method of directly inputting characters to the keyboard 73, or in a GUI (graphical user interface) environment,
A method of selecting from those displayed on the RT 72 using the mouse 74 may be used.

As described above, when the type of emitted light to be detected is input from the photographing method input means such as the keyboard 73, the switching means 60 responds to the type of the input emitted light, "chemiluminescent light". The combination of the irradiation light, the aperture, and the filter is determined with reference to a look-up table stored in the memory 71a of the computer 70. That is, as shown in Table 1, there is no irradiation light (all of the white LED light source 45a, the blue LED light source 45b, and the fluorescent lamp 45c are turned off), and the aperture is opened (the open aperture 41a is selected). ) And no filter (exclusion of the excitation light cut filter 42).

The switching means 60 retracts the excitation light cut filter 42 from the optical path in the direction of the arrow Y so that the obtained combination is obtained, and arranges the open stop 41a on the optical path.
Switching is performed so that none of the white LED light source 45a, the blue LED light source 45b, and the fluorescent lamp 45c is turned on.

The chemiluminescent light emitted from the sample 11 is imaged as an emission distribution image of the chemiluminescent light on the sample 11 by the lens 40 on the light receiving surface of the CCD 51 through the open aperture 41a. When an instruction to start exposure is input from the keyboard 73, the camera controller 5
2 controls exposure start for the CCD 51,
An exposure unit 51 performs exposure for a predetermined exposure time (for example, 30 minutes or the like) from the start of exposure, and reads the distribution image photoelectrically.
Then, the read image signal is transmitted to the camera controller 5.
The data is input to the personal computer 70 via the PC 2 and is used for image processing, quantitative analysis, and the like.

The personal computer 70 acquires the distribution image of the chemiluminescent light under image processing conditions suitable for performing image processing or the like based on the instruction of “chemiluminescent light” input as a photographing method from the keyboard 73. Image processing is performed on the received image signal.

When the acquisition of the image signal is completed, the operator opens the opening / closing door 25a of the photographing apparatus main body 20 and opens the sample 11
Is taken out of the dark box 25 together with the tray 10, whereby a series of photographing operations is completed.

Next, a case where an image of a sample 11 for detecting fluorescence (eg, a gel in which specific DNA labeled with a fluorescent dye is distributed) 11 is described.

First, as in the case of the sample for detecting the chemiluminescent light, the operator places the sample 11 for detecting the fluorescence at a predetermined position on the tray 10 and sets the tray 11 suitable for photographing at a desired angle of view. A rail is selected (in this embodiment, a tray rail 21c), and the tray 10 is arranged on the tray rail 21c.

When the tray 10 is placed by the operator, the sensor 22c corresponding to the third rail 21c on which the tray 10 is placed is placed on the tray 10 among the sensors 22a to 22g placed in the dark box 25. Is detected, the detection signal is sent to the lens moving means 32, and the lens moving means 32 receives the detection signal, recognizes that the detection signal has been output from the sensor 22c, and outputs the signal to the sensor 22c. The lens 40 is moved in the optical axis X direction to a position suitable for forming an image of the sample 11 on the tray 10 disposed on the corresponding third-stage rail 21c on the light receiving surface of the CCD 51 without defocusing. .

Next, the operator opens and closes the door 25 of the dark box 25.
a to close the dark box 25, and
Then, an input to the effect that the subject to be photographed is fluorescent light is input to the keyboard 73 of the “0”.

When the type of emitted light to be detected is input from the keyboard 73 in this manner, the switching means 60
The combination of the irradiation light, the diaphragm, and the filter corresponding to the type of the input emission light “fluorescence” is stored in the computer 70.
With reference to the look-up table stored in the memory 71a. That is, as shown in Table 1, "fluorescence"
And the blue LED light source 45 as the irradiation light
b, the combination of the first aperture 41b and the presence of a filter (using the excitation light cut filter 42) is obtained.

The switching means 60 controls the excitation light cut filter 42 and the first aperture 41 so that the obtained combination is obtained.
b is arranged on the optical path, and switching is performed so that the blue LED light source 45b is turned on.

Inside the dark box 25, the blue LED light source 45b
Is turned on, the blue LED light emitted from the blue LED light source 45b illuminates the sample 11, and excites a fluorescent dye that labels specific DNA distributed in the sample 11. Accordingly, fluorescence corresponding to the distribution of the specific DNA is emitted from the sample 11, and the emitted fluorescence is transmitted by the lens 40 onto the light receiving surface of the CCD 51 via the first aperture 41 b and the excitation light cut filter 42. When the operator inputs an instruction to start exposure from a keyboard 73, the camera controller 52 controls the CCD 51 to start exposure, and the CCD 51 controls the CCD 51 for a predetermined time from the start of exposure. Exposure is performed for the exposure time, and this distribution image is read photoelectrically. The read image signal is input to the personal computer 70 via the camera controller 52, and is used for image processing, quantitative analysis, and the like. Here, the sample 11
Blue LED reflected by sample 11 and tray 10
Although light is also incident, it is cut by the excitation light cut filter 42 and does not enter the CCD 51, and a distribution image of only emitted fluorescent light can be detected.

The personal computer 70 converts the acquired image signal into image signals under image processing conditions suitable for image processing or the like of the fluorescence distribution image based on the instruction of “fluorescence” input as an image capturing method from the keyboard 73. Then, image processing is performed.

When the acquisition of the image signal is completed, the operator opens the opening / closing door 25a of the photographing apparatus main body 20 and opens the sample 11
Is taken out of the dark box 25 together with the tray 10, whereby a series of photographing operations is completed.

Next, a case where a sample (for example, a reflection original such as a photograph) 11 for detecting reflected light is photographed as a digitizer will be described.

First, the operator places the sample 11 at a predetermined position on the tray 10, selects a tray rail suitable for photographing at a desired angle of view, and places the tray 10 on the tray rail.

When the tray 10 is placed by the operator, the sensor 22c corresponding to the rail (for example, 21c) on which the tray 10 is placed is placed on the tray 22 among the sensors 22a to 22g placed in the dark box 25. Is detected, and this detection signal is sent to the lens moving means 32,
The lens moving means 32 receives the detection signal, recognizes that the detection signal has been output from the sensor 22c, and sets the sample 11 on the tray 10 disposed on the third rail 21c corresponding to the sensor 22c. The lens 40 is moved in the direction of the optical axis X to a position suitable for forming the image on the light receiving surface of the CCD 51 without defocus.

Next, the operator opens and closes the door 25 of the dark box 25.
a to close the dark box 25, and
Then, an input is made to the keyboard 73 of No. 0 to indicate that the photographing target is reflected light.

When the type of emitted light to be detected is input from the keyboard 73 in this manner, the switching means 60
The combination of the irradiation light, the diaphragm, and the filter corresponding to the “reflected light” that is the type of the input light emission is
The value is obtained with reference to a look-up table stored in the memory 71a of the “0”. That is, from Table 1, a combination of the white LED light source 45a, the second diaphragm 41c, and the absence of the filter (retreating the excitation light cut filter 42) is obtained as the irradiation light, which is associated with the "reflected light".

The switching means 60 switches the excitation light cut filter 42 away from the optical path, arranges the second aperture 41c on the optical path, and turns on the white LED light source 45a so as to obtain the obtained combination. .

Inside the dark box 25, a white LED light source 45a
Is turned on, the white LED light emitted from the white LED light source 45a irradiates the sample 11, and the sample 11 emits reflected light according to the image recorded on the sample 11. The emitted reflected light is transmitted by the lens 40
The image is formed as a reflected light image of the image recorded on the sample 11 on the light receiving surface of the CCD 51 via the second aperture 41c. When the operator inputs an instruction to start exposure from the keyboard 73, the camera controller 52 , The exposure of the CCD 51 is performed for a predetermined exposure time from the start of the exposure, and the reflected light image is read photoelectrically. The read image signal is input to the personal computer 70 via the camera controller 52, and is used for image processing, quantitative analysis, and the like.

The personal computer 70 converts the acquired image signal into an image signal under image processing conditions suitable for image processing or the like of the reflected light image based on the instruction of “reflected light” input as a photographing method from the keyboard 73. Then, image processing is performed.

When the acquisition of the image signal is completed, the operator opens the opening / closing door 25a of the photographing apparatus main body 20 and opens the sample 11
Is taken out of the dark box 25 together with the tray 10, whereby a series of photographing operations is completed.

Next, a case where a sample for transmitted light detection (for example, a transparent original such as a positive film) 11 is photographed as a digitizer will be described.

First, the operator places the sample 11 at a predetermined position on the optically transparent tray 10, selects a tray rail suitable for photographing at a desired angle of view, and places the tray 10 on the tray rail. Place.

When the tray 10 is placed by the operator, the sensor 22b corresponding to the rail (for example, 21b) on which the tray 10 is placed among the sensors 22a to 22g placed in the dark box 25 is placed on the tray 10. Is detected, and this detection signal is sent to the lens moving means 32,
The lens moving means 32 receives the detection signal, recognizes that the detection signal has been output from the sensor 22b, and detects the sample 11 on the tray 10 disposed on the second rail 21b corresponding to the sensor 22b. The lens 40 is moved in the direction of the optical axis X to a position suitable for forming the image on the light receiving surface of the CCD 51 without defocus.

Next, the operator opens and closes the door 25 of the dark box 25.
a to close the dark box 25, and
An input is made to the keyboard 73 of No. 0 to indicate that the object to be photographed is transmitted light.

When the type of the emitted light to be detected is input from the keyboard 73 in this manner, the switching means 60
The combination of the irradiation light, the diaphragm, and the filter corresponding to the type of the transmitted light “transmission light” is transmitted to the computer 7.
The value is obtained with reference to a look-up table stored in the memory 71a of the “0”. That is, from Table 1, the fluorescent lamp 45c, the second diaphragm 41c, and no filter (the excitation light cut filter 4
2) is acquired.

The switching means 60 retracts the excitation light cut filter 42 from the optical path, arranges the second diaphragm 41c on the optical path, and sets the fluorescent lamp disposed below the sample 11 so as to obtain the obtained combination. 45c is turned on.

Since the fluorescent lamp 45c is turned on inside the dark box 25, the white light emitted from the fluorescent lamp 45c passes through the transparent tray 10 to irradiate the sample 11, and the sample 11 records the sample 11. The transmitted light corresponding to the image thus emitted is emitted. The emitted transmitted light is imaged by the lens 40 on the light receiving surface of the CCD 51 through the second aperture 41 c as a transmitted light image of an image recorded on the sample 11, where the operator When a start instruction is input, the camera controller 52
D51 is controlled to start exposure, and the CCD 51 performs exposure for a predetermined exposure time from the start of exposure, and photoelectrically reads the transmitted light image. The read image signal is input to the personal computer 70 via the camera controller 52, and is used for image processing, quantitative analysis, and the like.

The personal computer 70 converts the transmitted image signal into an image signal under image processing conditions suitable for image processing or the like of the transmitted light image based on an instruction of “transmitted light” input as a photographing method from the keyboard 73. Then, image processing is performed.

When the acquisition of the image signal is completed, the operator opens the opening / closing door 25a of the photographing apparatus main body 20 and opens the sample 11
Is taken out of the dark box 25 together with the tray 10, whereby a series of photographing operations is completed.

[Brief description of the drawings]

FIG. 1 is a diagram showing an entire configuration of an embodiment of a photographing apparatus of the present invention.

FIG. 2 is a perspective view showing a schematic configuration of the photographing apparatus shown in FIG. 1;

[Explanation of symbols]

 Reference Signs List 10 tray 11 sample 20 imaging apparatus main body 21a to 21g tray rail 22a to 22g tray sensor 25 dark box 25a opening and closing door 32 lens moving means 40 lens 41 aperture 41a open aperture 41b first aperture 41c second aperture 42 excitation light cut filter 45a white LED Light source 45b Blue LED light source 45c Fluorescent lamp 51 Cooling CCD 52 Camera controller 60 Switching means 70 Personal computer 71 Computer body 71a Memory 72 CRT 73 Keyboard 74 Mouse 100 Imaging device

Continued on the front page F-term (reference) 2G020 BA03 CA01 CC09 CC26 CD03 CD11 CD22 CD34 CD37 CD51 DA12 DA42 2G043 AA03 BA16 CA03 DA02 DA06 EA01 FA01 GA04 GB01 GB18 GB19 GB21 HA01 JA03 LA03 MA01 NA06 NA13 2G054 AA03 CA22 CA23 CD01 CE02 EA01 FA19 FA33 FB01 GB01 GB02 JA02 JA05 JA10 2H002 AB01 CC21 EB17 FB21 FB24 FB28 GA35 HA11 HA12 HA13 JA00 JA11 5C022 AA01 AB12 AB13 AB15 AC00 AC31 AC55 CA07

Claims (4)

[Claims] 1. A type of light irradiating the sample according to the type of the emitted light, wherein the type of light emitted to the sample is arranged between the sample and the photoelectric reading means. In the imaging method in which the degree of the aperture to be switched and the type of the filter can be switched, the type of the light to be irradiated, the degree of the aperture to be disposed, and the arrangement are determined according to the type of the emitted light to be detected. The combination of the types of the filters is stored in a reference table in advance, and when the selection of the type of the emitted light to be detected is input, the type of the light to be irradiated according to the type of the selected emitted light is received. The combination of the degree of the aperture to be arranged and the type of the filter to be arranged is determined by referring to the reference table. According to the determined combination, the light to be radiated is determined. A photographing method, wherein the type of the lens, the degree of the aperture to be arranged, and the type of the filter to be arranged are switched. 2. The reference table according to claim 1, wherein when the emitted light to be detected is chemiluminescent light, there is no irradiating light, the arranged aperture is open, and the arranged filter is absent. When the emitted light to be detected is fluorescence, the light to be irradiated is excitation light for exciting the fluorescence, the aperture to be arranged is a first aperture state narrower than the open state, and the arrangement is arranged. The filter cuts the excitation light, and is a combination of an excitation light cut filter whose band is set so as to transmit the fluorescence, and when the emitted light to be detected is reflected light or transmitted light from the sample. Corresponds to a combination that the light to be irradiated is white light, the aperture to be arranged is a second aperture state further narrowed than the first aperture state, and the filter to be arranged is absent. The method of imaging according to claim 1, characterized in that it is an eclipse. 3. A type of light irradiating the sample according to the type of the emitted light, wherein the type of light emitted to the sample is arranged between the sample and the photoelectric reading unit. In a photographing apparatus in which the degree of the aperture to be switched and the type of the filter can be respectively switched, the type of the light to be irradiated, the degree of the aperture to be disposed and the arrangement are determined according to the type of the emitted light to be detected. Storage means for storing a combination of the types of filters in advance as a reference table; photographing method input means for inputting selection of the type of the emitted light to be detected; and inputting the photographing method with reference to the reference table. The combination of the type of the light to be irradiated, the degree of the aperture to be arranged, and the type of the filter to be arranged, corresponding to the type of the emitted light input to the means, A photographing apparatus, comprising: switching means for switching the type of light to be irradiated, the degree of the aperture to be arranged, and the type of the filter to be arranged, in accordance with the obtained combination. 4. When the reference table indicates that the emitted light to be detected is chemiluminescent light, there is no light to be applied, the aperture to be arranged is in an open state, and the filter to be arranged is no combination. When the emitted light to be detected is fluorescence, the light to be irradiated is excitation light for exciting the fluorescence, the aperture to be arranged is a first aperture state narrower than the open state, and the arrangement is arranged. The filter cuts the excitation light, and is a combination of an excitation light cut filter whose band is set so as to transmit the fluorescence, and when the emitted light to be detected is reflected light or transmitted light from the sample. Corresponds to a combination that the light to be irradiated is white light, the aperture to be arranged is a second aperture state further narrowed than the first aperture state, and the filter to be arranged is absent. Photographing apparatus according to claim 3, wherein a is obtained vignetting.