JPH10210344A - Cooled ccd camera - Google Patents

Abstract

PROBLEM TO BE SOLVED: To easily assemble the members of a cooled CCD(charge coupled device) camera, so as to fix the optical axis especially the focal point of the CCD despite the uneven dimensions, particularly the uneven height directions of the members. SOLUTION: This camera includes a CCD 41, a heat exchanger plate 42, a cooling element 43 which holds the CCD 41, a substrate 45 which contains the electronic parts and an airtight metallic package 40 which contains these component elements. Then at least one of a terminal 46 of the CCD 41 and a connector terminal 47 which secures connection to the outside is fixed into a through-hole of a socket fixed in a hole formed at a prescribed point of the substrate 45 and then connected to the substrate 45 via the socket.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cooled CCD camera, and more particularly, to a CCD camera (charge-coupled device) having a constant optical axis even when the dimensions of the members vary. Cooled CCD that can be easily assembled
It is about cameras.

[0002]

2. Description of the Related Art An immobilized polymer such as a protein or nucleic acid sequence is selectively labeled with a labeling substance that causes chemiluminescence upon contact with a chemiluminescent substance, and is selectively labeled with the labeling substance. The polymer is brought into contact with the chemiluminescent substance, and the chemiluminescence in the visible light wavelength region caused by the contact between the chemiluminescent substance and the labeling substance is photoelectrically detected, and a digital image signal is generated to perform image processing. A chemiluminescence detection system is known in which a chemiluminescence image is reproduced on a display means such as a CRT or a recording material such as a photographic film to obtain information on a macromolecule such as genetic information. Also, a fluorescence detection system using a fluorescent substance as a labeling substance is known. According to this fluorescence detection system, by reading a fluorescence image, it is possible to perform gene sequence, gene expression level, protein separation and identification, or molecular weight and property evaluation. D
After adding a fluorescent dye to the solution containing the NA fragment, a plurality of DNA fragments are subjected to electrophoresis on a gel support, or a plurality of DNA fragments are placed on a gel support containing a fluorescent dye.
The A fragment is electrophoresed, or a plurality of DNA fragments are electrophoresed on a gel support, and then the gel support is immersed in a solution containing a fluorescent dye. By labeling, exciting a fluorescent dye with excitation light, and detecting the generated fluorescence, an image is generated, and the distribution of DNA on the gel support is detected. After electrophoresis on a support, the DNA is denaturated and then
A probe prepared by transferring at least a portion of a denatured DNA fragment onto a transfer support such as nitrocellulose by Southern blotting, and labeling DNA or RNA complementary to the target DNA with a fluorescent dye D
An image is generated by hybridizing with an NA fragment, selectively labeling only a DNA fragment complementary to a probe DNA or a probe RNA, exciting a fluorescent dye with excitation light, and detecting generated fluorescence. Then, the distribution of the target DNA on the transcription support can be detected. Further, a DNA probe complementary to the DNA containing the target gene labeled with the labeling substance is prepared, hybridized with the DNA on the transcription support, and the enzyme is reacted with the complementary DNA labeled with the labeling substance. After binding, the fluorescent substance is brought into contact with a fluorescent substrate, the fluorescent substrate is changed into a fluorescent substance that emits fluorescence, and the generated fluorescent substance is excited by excitation light, and the generated fluorescence is detected.
An image can be generated to detect the distribution of the target DNA on the transfer support. This fluorescence detection system has an advantage that a gene sequence or the like can be easily detected without using a radioactive substance.

[0003] Such a chemiluminescent or fluorescent light is cooled by a cooled CCD.
When detecting with a camera and generating a chemiluminescent image or fluorescent image, chemiluminescent or fluorescent light is very weak light, so it is necessary to perform exposure for a long time. , Due to the heat generated by the CCD
It is known that noise occurs in an image. In order to reduce such heat-induced noise, a cooled CCD camera for detecting very weak light such as chemiluminescence or fluorescence is provided in a hermetically sealed metal package in a CCD or metal package. It includes a hot plate, a cooling element for cooling the CCD, a substrate on which electronic components are mounted, and the like.

[0004]

However, CC
D, It is extremely difficult to manufacture heat transfer plates, cooling elements, substrates, etc., so that their sizes are always constant, and it is inevitable that the sizes of these members will vary. When the CCD and these members are assembled and the terminal of the CCD and the external connector terminal are connected to the substrate, it is difficult to keep the optical axis of the CCD constant. A similar problem occurs when a cooled CCD camera is used to generate an image by detecting very weak light, such as in astronomical observation. Therefore, the present invention provides a cooled CCD camera that can be easily assembled so that the optical axis of the CCD, particularly the focal position, is constant even if the dimensions of the member, especially the height of the member, vary. The purpose is to do so.

[0005]

SUMMARY OF THE INVENTION An object of the present invention is to provide a CCD, a heat transfer plate, a cooling element for cooling the CCD, a substrate on which electronic components are mounted, and a metal package having an airtight structure for accommodating these components. A cooled CCD camera, said CCD
At least one of the terminal and the connector terminal with the outside is fixed in the through-hole of the socket having a through-hole fixed in a hole formed in a predetermined portion of the substrate,
A cooling CC connected to the board via the socket
Achieved by a D camera. According to the present invention, the CCD
Terminal or a connector terminal with the outside is configured to be connected to the substrate in a through hole of a socket having a through hole fixed in a hole formed in a predetermined portion of the substrate, so that the CCD, Even if there are variations in the size of members such as the heat transfer plate and the cooling element that cools the CCD, and the board on which the electronic components are mounted, and especially in the height direction of the members, at least one of the CCD terminals and the external connector terminals should be used. It can be moved in the through hole of the socket and fixed to the socket at a position corresponding to the variation in the size of these members, and can be connected to the substrate via the socket. It is possible to keep the optical axis of the CCD of the cooled CCD camera, particularly the focal position, always constant.

[0006]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Preferred embodiments according to the present invention will be described below in detail with reference to the accompanying drawings. FIG. 1 shows a cooling C according to a preferred embodiment of the present invention.
1 is a schematic front view of an image generation device including a CD camera. FIG.
1, the image generation device includes an imaging device 1, a dark box 2, and a personal computer 4. As shown in FIG. 1, the personal computer 3 includes a CRT 4 and a keyboard 5. FIG. 2 is a schematic vertical sectional view of the imaging device 1. As shown in FIG. 2, the imaging device 1 includes a cooled CCD camera 6 and an A that converts analog image data generated by the cooled CCD camera 6 into digital image data.
A / D converter 7, an image data buffer 8 for temporarily storing image data digitized by the A / D converter 7, and a camera control circuit 9 for controlling the operation of the imaging apparatus 1
And Radiation fins 10 for radiating heat are formed around the imaging device 1 over substantially half of the longitudinal direction. On the wall surface 10a of the radiation fin 10,
The cooling CCD camera 6 is fixed in close contact, and heat from the cooling CCD camera 6 is radiated.

[0007] A shutter 11 is provided in front of the cooled CCD camera 6 provided in the imaging apparatus 1, and an image intensifier 12 is provided with a dark box 2 in front of the imaging apparatus 1.
A camera lens 13 is attached to the front of the image intensifier 12. FIG.
Is a schematic vertical sectional view of the dark box 2. FIG. As shown in FIG. 3, a first blue LED light source 21 that emits excitation light having an emission wavelength center of 450 nm is provided in the dark box 2.
Obliquely above the first blue LED light source 21, a second blue LED light source 22 and a third blue LED light source 23 that emit excitation light having an emission wavelength center of 450 nm are provided. A filter 2 is provided on the upper surface of the first blue LED light source 21.
4 are attached to the front surface of the second blue LED light source 22 and the third blue LED light source 23, respectively.
5 and a filter 26 are attached. Filter 2
Nos. 4, 25 and 26 have a property of cutting out light harmful to excitation of a fluorescent substance other than a wavelength of around 450 nm and transmitting only light of a wavelength of around 450 nm. Camera lens 1
A filter 27 that cuts off the excitation light near 450 nm is provided on the front surface of 3 in a detachable manner.

FIG. 4 is a block diagram of the periphery of the personal computer 3. As shown in FIG. 4, the personal computer 3 includes a CPU 30 for controlling the exposure of the imaging device 1, an image data transfer unit 31 for reading out image data generated by the imaging device 1 from the image data buffer 8, and an image data transfer unit. An image processing unit 33 performs image processing on the image data read by the image data storage unit 31 and stores the image data in an image data storage unit 32. Based on the image data stored in the image data storage unit 32, a visible image is displayed on the screen of the CRT 4. Image display means 34 for displaying. First blue LED light source 21, second blue LED
The light source 22 and the third blue LED light source 23 are controlled by a light source control unit 35, and an instruction signal is input to the light source control unit 35 from the keyboard 5 via the CPU 30. . The CPU 30 is configured to be able to output various signals to the camera control circuit 9 of the imaging device 1.

FIG. 5 is a schematic vertical sectional view showing details of the cooled CCD camera 6. As shown in FIG. As shown in FIG.
The camera 6 includes an aluminum alloy package 40 having an airtight structure. Inside the package 40, a CCD 41 and a heat transfer plate 42 made of a metal such as an aluminum alloy are provided.
A Peltier element 43 for cooling the CCD 41;
The CCD 41 includes an optical glass plate 44 attached to a package 40 on the camera lens 13 side of the CCD 41, and a substrate 45 on which electronic components are mounted. The terminal 46 of the CCD 41 is connected to a predetermined portion of the substrate 45 via a socket described later,
An external connector terminal 47 is connected to a predetermined portion of the substrate 45 via a socket also described later. The melting point between the connector terminal 47 and the package 40 and between the optical glass plate 44 and the package 40 are 4
Hermetically sealed with low melting point glass of 00 to 450 ° C.

FIG. 6 is a schematic side view showing the connection between the substrate 46 and the terminals 46 and connector terminals 47 of the CCD 41. As shown in FIG. 6, the terminal 46 and the connector terminal 47 of the CCD 41 are both connected to predetermined portions of the substrate 45 via a socket 51 having a through hole 50. Each socket 51 is fixed to a hole 52 formed in the substrate 45. FIG. 7 is a schematic perspective view of the socket 51. Before assembling, the terminal 46 and the connector terminal 47 of the CCD 41 are movable in a through hole 50 formed in the socket 51. ,
It is configured to be fixed in the socket 51 at a position determined by the thickness of members such as the Peltier element 43 and the substrate 45.

An image generating apparatus including a cooled CCD camera according to the present embodiment detects fluorescence from an image carrier carrying an image of a fluorescent substance and chemiluminescence generated by contact of a chemiluminescent substance with a labeling substance, It is configured to be able to generate a fluorescence image and a chemiluminescence image. When a fluorescent image is generated, a fluorescent image is detected from an image carrier carrying an image of a fluorescent substance, and a visible image is generated as described below. Here, the image carrier carries an image of a fluorescent substance means that the image carrier carries an image of a sample labeled with a fluorescent dye, and that the enzyme is combined with the labeled sample before the enzyme is fluoresced. Contacting with a substrate to change the fluorescent substrate into a fluorescent substance that emits fluorescence, and carrying an image of the obtained fluorescent substance.

First, after the user places the image carrier 18 as a sample on the filter 24, focuses on the filter, and closes the dark box 2, the user inputs an exposure start signal to the keyboard 5; The first blue LED light source 21 or the second blue LED light source 22 and the third blue LED light source 23
Is turned on, and the excitation light is emitted toward the image carrier 18. At the same time, the exposure start signal is
Input to the camera control circuit 9 of the imaging device 1
Thereby, the shutter 11 is opened, and the exposure of the CCD 41 is started. Excitation light emitted from the first blue LED light source 21 or the second blue LED light source 22 and the third blue LED light source 23 has wavelength components other than light having a wavelength near 450 nm by filters 24, 25, and 26. As a result, the fluorescent substance in the image carrier 18 is excited by light having a wavelength near 450 nm, and emits fluorescence.

The fluorescent light emitted from the fluorescent substance in the image carrier 18 enters the photoelectric surface of the image intensifier 12 via the filter 27 and the camera lens 13, is amplified, and is amplified. An image is formed on the fluorescent screen of. The CCD 41 of the imaging device 1
Thus, the light of the image formed on the phosphor screen of the image intensifier 12 is received, and the light is accumulated in the form of electric charges. Since the filter 27 cuts off the excitation light having a wavelength near 450 nm, only the fluorescent light emitted from the fluorescent substance in the image carrier 18 is emitted from the CCD of the imaging device 1.
Light is received by 41. When the predetermined exposure time has elapsed, the CPU 30 outputs an exposure completion signal to the camera control circuit 9 of the imaging device 1. The camera control circuit 9 includes the CPU 3
When the exposure completion signal is received from 0, the analog image data accumulated in the form of electric charges by the CCD 41 of the cooled CCD camera 6 is transferred to the A / D converter 7, digitized, and temporarily stored in the image data buffer 8. Let it. At the same time, CP
U30 outputs a data transfer signal to the image data transfer means 31, reads out digital image data temporarily stored in the image data buffer 8 of the imaging device 1, and inputs the digital image data to the image processing means 33. The image processing unit 33 performs image processing on the image data input from the image data transfer unit 31 and causes the image data storage unit 32 to store the image data.

Thereafter, when the user inputs an image generation signal to the keyboard 5, the image display means 35 reads the image data stored in the data storage means 33, and based on the read image data, the CRT 4 A fluorescent image is displayed on the screen. When generating a chemiluminescent image, the filter 27 is removed, and the first blue LED light source 21, the second blue LED light source 22, and the third blue LED
Each of the ED light sources 23 is kept in the off state, and the filter 2
4 except that a sample 18 that emits chemiluminescence is placed thereon and the chemiluminescence emitted from the sample 18 is detected.
Sample 1 was prepared in exactly the same way as when the fluorescent image was generated.
Chemiluminescence emitted from the CCD 4 through the camera lens 13 and the image intensifier 12
1 to photoelectrically detect and generate image data,
A chemiluminescence image is displayed on the screen of RT4.

According to this embodiment, before assembly, C
The terminals 46 and the connector terminals 47 of the CD 41 are movable in through holes 50 formed in the socket 51, and at a position determined by the thickness of members such as the heat transfer plate 42, the Peltier element 43, and the substrate 45 at the time of assembly. , Socket 51
The CCD 41, the heat transfer plate 42, the Peltier element 4
3. Even if the thickness of the members such as the substrate 45 varies,
By easily assembling these members, it is possible to obtain a cooled CCD camera 6 in which the optical axis of the CCD 41 is always constant. The present invention is not limited to the above embodiments,
Various modifications are possible within the scope of the invention described in the claims, and it goes without saying that they are also included in the scope of the present invention.

For example, in the above embodiment, CC
Although both the terminal 46 of D41 and the connector terminal 47 are connected to the board 45 via the socket 51,
At least one of the terminal 46 and the connector terminal 47 of the CD 41 may be connected to the board 45 via the socket 51. Further, in the above embodiment, the image intensifier 12 is provided on the front surface of the imaging device 1, but it is not always necessary to provide the image intensifier 12. Further, in the above embodiment, the first blue LED light source 21, the second blue LED light source 22, and the third blue LED light source 2
3, the first blue LED light source 21 alone, or the second blue LED light source 22 and the third blue LED
Only the ED light source 23 may be provided.

In the above embodiment, the blue LED light source 2 emits excitation light having a light emission wavelength center of 450 nm.
Although 1, 22, and 23 are used, an LED light source that emits excitation light having a wavelength of 400 to 700 nm can be selected and used according to the type of the fluorescent substance. Further, in the above embodiment, when an exposure start signal is inputted to the keyboard 5, the light source control means 35
Thus, the first blue LED light source 21 or the second blue LED light source 22 and the third blue LED light source 23 are turned on, but the light source control means 35 is controlled by the personal computer 3. It is not always necessary to configure, and the light source control means 35 may be manually operated.

In the above embodiment, the image generating apparatus is configured such that the filter 27 for cutting off the excitation light near 450 nm is detachable, and by removing the filter 27, the extremely weak chemiluminescence is detected. Therefore, it is configured to be able to generate a chemiluminescence image,
The filter 27 may be fixedly provided on the front surface of the camera lens 13 so as to generate only a fluorescence image in the fluorescence detection system. Further, in the above embodiment, the first blue LED light source 21 and the second blue LE
D light source 22 and a third blue LED light source 23. When used as an image generating apparatus that detects chemiluminescence and generates only a chemiluminescence image, the first blue L
The ED light source 21, the second blue LED light source 22, and the third blue LED light source 23 are not required.
There is no need for 4, 25, 26, 27 either.

In the above embodiment, the radiation fins 10 for dissipating the heat generated by the Peltier element 8 are formed around the imaging device 1 over almost half of the longitudinal direction. May be provided over all of them, and how much the heat radiation fins 10 are provided around the imaging device 1 can be arbitrarily determined.

[0020]

According to the present invention, cooling which can be easily assembled so that the optical axis of the CCD, particularly the focal position, becomes constant even if the dimensions of the members vary, especially in the height direction of the members. It is possible to provide a CCD camera.

[Brief description of the drawings]

FIG. 1 is a schematic front view of an image generating apparatus including a cooled CCD camera according to a preferred embodiment of the present invention.

FIG. 2 is a schematic vertical sectional view of the imaging apparatus.

FIG. 3 is a schematic vertical sectional view of a dark box.

FIG. 4 is a block diagram of the periphery of a personal computer.

FIG. 5 is a schematic vertical sectional view showing details of a cooled CCD camera.

FIG. 6 is a schematic side view showing a connection mode between a terminal and a connector terminal of a CCD and a substrate.

FIG. 7 is a schematic perspective view of a socket.

[Explanation of symbols]

 REFERENCE SIGNS LIST 1 imaging device 2 dark box 3 personal computer 4 CRT 5 keyboard 6 cooled CCD camera 7 A / D converter 8 image data buffer 9 camera control circuit 10 radiating fin 11 shutter 12 image intensifier 13 camera lens 21 first blue LED Light source 22 Second blue LED light source 23 Third blue LED light source 24, 25, 26, 27 Filter 30 CPU 31 Image data transfer means 32 Image data storage means 33 Image processing means 34 Image display means 35 Light source control means 40 Aluminum alloy Package 41 CCD 42 Heat transfer plate 43 Peltier element 44 Optical glass plate 45 Substrate 46 CCD terminal 47 Connector terminal 50 Through hole 51 Socket 52 hole

Claims (1)

[Claims] 1. A charge coupled device (CCD), a heat transfer plate,
A cooled CCD camera comprising a cooling element for cooling the CCD, a substrate on which electronic components are mounted, and a metal package having an airtight structure for accommodating the electronic device, wherein at least a terminal of the CCD and a connector terminal for the outside. One is fixed in the through-hole of a socket having a through-hole fixed in a hole formed in a predetermined portion of the substrate, and is connected to the substrate via the socket. Cooled CCD camera.