CN112540417A - Device and method for detecting existence of consumable materials in cell sorter - Google Patents
Device and method for detecting existence of consumable materials in cell sorter Download PDFInfo
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- CN112540417A CN112540417A CN202011575038.3A CN202011575038A CN112540417A CN 112540417 A CN112540417 A CN 112540417A CN 202011575038 A CN202011575038 A CN 202011575038A CN 112540417 A CN112540417 A CN 112540417A
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- 239000000463 material Substances 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims description 18
- 239000013307 optical fiber Substances 0.000 claims abstract description 80
- 238000001514 detection method Methods 0.000 claims abstract description 21
- 239000000835 fiber Substances 0.000 claims abstract description 6
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- 238000005259 measurement Methods 0.000 claims description 4
- 210000004027 cell Anatomy 0.000 description 29
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 10
- 230000000712 assembly Effects 0.000 description 5
- 238000000429 assembly Methods 0.000 description 5
- 238000001914 filtration Methods 0.000 description 4
- 210000004881 tumor cell Anatomy 0.000 description 4
- 206010027476 Metastases Diseases 0.000 description 3
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- 230000002980 postoperative effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 1
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- 230000003631 expected effect Effects 0.000 description 1
- 238000003316 immunomagnetic separation method Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01V—GEOPHYSICS; GRAVITATIONAL MEASUREMENTS; DETECTING MASSES OR OBJECTS; TAGS
- G01V8/00—Prospecting or detecting by optical means
- G01V8/10—Detecting, e.g. by using light barriers
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Abstract
The invention discloses a device for detecting the existence of consumables in a cell sorter, which comprises an X-axis assembly, a Y-axis assembly and a Z-axis assembly which are arranged in the cell sorter, wherein an optical fiber assembly for detecting the existence of consumables is arranged on the Z-axis assembly, and the optical fiber assembly is suitable for adjusting position coordinates in the X-axis direction, the Y-axis direction and the Z-axis direction in a stroke adjusting interval of the X-axis assembly, the Y-axis assembly and the Z-axis assembly; the cell sorter is located the below in X axle subassembly, Y axle subassembly, Z axle subassembly's coordinate regulation district is provided with the consumptive material and places the district, the optic fibre subassembly is suitable for the adjustment to the top of district arbitrary position is placed to the consumptive material. The device for detecting whether consumables exist in the cell sorter quickly judges whether consumables are placed on stations of each consumable placing area, so that the detection efficiency and the detection accuracy are improved.
Description
Technical Field
The invention relates to the technical field of cell sorters, in particular to a device and a method for detecting whether consumables exist in a cell sorter.
Background
Circulating Tumor Cells (CTCs) are tumor cells that are released into the peripheral blood circulation from solid tumors or metastases, either spontaneously or as a result of medical procedures, and are important causes of postoperative recurrence and distant metastasis in malignant patients. In recent years, with the continuous improvement of detection technology, the application value of CTCs detection as a novel non-invasive diagnostic tool in the aspects of early detection of postoperative recurrence and distant metastasis of patients, evaluation of curative effect and prognosis and the like has become a hot spot of clinical research. However, since the number of CTCs in peripheral blood is very small, and the only few tumor cells are usually found in about 1 million white blood cells and 500 million red blood cells, the CTCs are usually enriched before detection, i.e., they are separated from collected blood or other samples, in order to increase the detection rate of the CTCs.
Currently, the enrichment methods of CTCs are mainly classified into immunomagnetic separation methods and enrichment methods based on the physical properties of CTCs according to their principles. Among them, enrichment methods based on the physical properties of CTCs mainly include density gradient centrifugation and filtration. The filtration method is a method for separating tumor cells based on their sizes, and is mainly used for separating tumor cells according to their sizes from normal cells. The filtration method is a method for separating cells by a physical method, so that the cell morphology can be well and completely preserved, various antigens or molecular markers on the surface are not damaged, the characteristics of the cells are not influenced, good conditions are provided for subsequent detection, the technical requirement on equipment is not high, the process is easy to master, and the method becomes the most common CTCs enrichment method in practical operation.
The cell sorter used in the filtration method is a 24-channel full-automatic sorter, wherein the 24 channels are divided into 4 groups and 6 channels, and the sample adding process of each group of six channels is linked; the kit is a single-use kit, consumables required by the cell sorter comprise a gun head, a cell filter, the single-use kit, a sample, a glass slide and the like, and the consumables are required to be manually placed by a user; the mismatching of the number or the placement position of any consumable may cause the cell sorting result to fail to achieve the expected effect, but the prior art has not yet researched and developed the technology of whether the relevant consumable is placed in place in the cell sorter, so that the technical gap needs to be filled.
Disclosure of Invention
Therefore, the technical problem to be solved by the present invention is to overcome the above-mentioned deficiencies of the existing cell sorter technology, and further to provide a device and a method for detecting the presence or absence of consumables in a cell sorter.
In order to achieve the purpose, the invention adopts the following technical scheme:
a device for detecting the existence of consumables in a cell sorter comprises an X-axis assembly, a Y-axis assembly and a Z-axis assembly which are arranged in the cell sorter, wherein an optical fiber assembly for detecting the existence of consumables is arranged on the Z-axis assembly, and the optical fiber assembly is suitable for adjusting position coordinates in the X-axis direction, the Y-axis direction and the Z-axis direction in a stroke adjusting interval of the X-axis assembly, the Y-axis assembly and the Z-axis assembly; the cell sorter is located the below in X axle subassembly, Y axle subassembly, Z axle subassembly's coordinate regulation district is provided with the consumptive material and places the district, the optic fibre subassembly is suitable for the adjustment to the top of district arbitrary position is placed to the consumptive material.
Preferably, the optical fiber assembly is provided with at least one group, and each group of the optical fiber assembly comprises an optical fiber amplifier and an optical fiber sensor.
Preferably, the optical fiber amplifier is arranged on the Z-axis assembly, and the optical fiber sensor is adapted to perform position coordinate adjustment along the X-axis direction, the Y-axis direction, and the Z-axis direction.
Preferably, the optical fiber core diameter of the optical fiber component ranges from 0.1mm to 5 mm.
Preferably, the color of the emitted light of the optical fiber assembly is at least one of red light, blue light, green light and white light.
Preferably, the cell sorter further comprises a display for displaying the detection result, and the display is fixedly arranged on the body of the cell sorter.
Preferably, the consumable material placing area comprises at least one of a kit placing area, a gun head placing area, a filter placing area, a blood collection tube placing area, a kit placing area and a slide placing area.
A method for detecting the presence or absence of a consumable in a cell sorter as claimed in any of the above claims, comprising the steps of:
s1: moving the X shaft assembly, the Y shaft assembly and the Z shaft assembly to enable an optical fiber sensor in an optical fiber assembly on the Z shaft assembly to move to the position above a consumable to be detected; observing the measured values of the optical fiber amplifiers in the optical fiber assembly under the condition that the consumables are placed and the consumables are not placed respectively; adjusting the power and corresponding time of the optical fiber amplifier and the height of the Z-axis component to enable the measurement of the optical fiber amplifier in the state of placing consumables to be more than or equal to half of a full range, and setting the value as a corresponding value of the maximum value of the analog quantity;
s2: corresponding consumables are respectively placed in the gun head placing area, the filter placing area, the blood collection tube placing area and the glass slide placing area, and consumables in all the areas are not full;
s3: running a consumable detection program, waiting for the detection to be completed, observing all detection values on a display screen, and setting a judgment threshold value for whether each consumable exists or not;
s4: and judging whether a threshold value exists or not according to each consumable material, and distinguishing whether each station places a corresponding consumable material or not.
If more than two groups of optical fiber assemblies are arranged on the Z-axis assembly, the step S2 is repeated to set the corresponding value of the maximum analog quantity value of the optical fiber amplifier in each group of optical fiber assemblies.
The invention has the beneficial effects that:
according to the device for detecting the existence of the consumables in the cell sorter, the optical fiber sensor and the optical fiber transmission amplifier are arranged, and whether the consumables are placed on the stations of the consumable placing areas or not is rapidly judged based on the optical fiber signals transmitted by the optical fiber assembly and the optical signal received by the optical fiber assembly, so that the inspectors can master the existence of the consumables greatly conveniently, and the detection efficiency and the detection accuracy are improved.
Drawings
In order that the present invention may be more readily and clearly understood, reference is now made to the following detailed description taken in conjunction with the accompanying drawings, in which:
FIG. 1 is a schematic view showing the structure of an apparatus for detecting the presence or absence of consumables in a cell sorter according to the present invention;
FIG. 2 is a schematic view showing another perspective (the opposite direction to the perspective in FIG. 1) of the apparatus for detecting the presence or absence of consumables in the cell sorter according to the present invention.
The reference numbers in the figures denote:
100-cell sorter; 1-X axis assembly; 2-Y shaft assembly; 3- -Z shaft assembly; 4-a kit placement area; 5-glass slide placing area; 6-a gun head placing area; 7-blood collection tube placement area; 8-a filter placement zone; 9. 10-a fiber amplifier; 11. 12-fiber optic sensor.
Detailed Description
Example one
Referring to fig. 1-2, the device for detecting the presence or absence of consumables in the cell sorter of this embodiment includes an X-axis module 1, a Y-axis module 2, a Z-axis module 3, and a consumable placement area that are disposed in the body of the cell sorter 100, where the consumable placement area includes a kit placement area 4, a slide placement area 5, a tip placement area 6, a blood collection tube placement area 7, a filter placement area 8, and two sets of optical fiber assemblies that are disposed on the Z-axis module, where one set of optical fiber assemblies includes an optical fiber amplifier 9 and an optical fiber sensor 11, the other set of optical fiber assemblies includes an optical fiber amplifier 10 and an optical fiber sensor 12, the optical fiber sensor 11 is electrically connected to the optical fiber amplifier 9, and the optical fiber sensor 12 is electrically connected to the; the optical fiber sensor 11 and the optical fiber sensor 12 are arranged on the Z-axis component 2 and are suitable for sequentially moving to the detection positions of the consumable placing areas along the X-axis direction, the Y-axis direction and the Z-axis direction respectively.
The specific structures and compositions of the X-axis assembly, the Y-axis assembly and the Z-axis assembly in this embodiment are not limited as long as adaptive coordinate adjustment of the optical fiber sensors 11 and 12 in the coordinate system stroke can be achieved. The optical fiber assembly of the present embodiment may use an existing known optical fiber sensor, such as a conventional optical fiber amplifier with analog output, the optical fiber assembly of the present embodiment preferably has an optical fiber core diameter ranging from 0.1mm to 5mm, the color of light emitted by the optical fiber assembly is any one of red light, blue light, green light and white light, and the color of optical fibers of different colors does not affect the detection result.
The device of this embodiment detect consumptive material and have in the cell sorter still is including the display screen that is used for showing the monitoring result, the display screen can be solitary display screen, also can share the display screen with the cell sorter, the display screen can be through control and transmission of control circuit board, and then shows in real time the consumptive material has or not testing result to supply the inspection personnel to observe.
Example two
Referring to fig. 1-2, the method for detecting the presence or absence of a consumable in a cell sorter according to the first embodiment includes the steps of:
s1: moving the X-axis assembly 1, the Y-axis assembly 2 and the Z-axis assembly 3 to enable the optical fiber sensor 11 (the model is PRA3Y10QD) in the Z-axis assembly 3 to move to the position above the consumable to be detected; observing the measured values of the fiber amplifier 9 (model number ER2-22HM) with and without consumables placed, respectively; adjusting the power and corresponding time of the optical fiber amplifier and the height of the Z-axis component 3 to enable the measurement of the optical fiber amplifier in the state of placing consumables to be more than or equal to half of a full range, and setting the value as a corresponding value of the maximum value of the analog quantity;
moving the X-axis assembly 1, the Y-axis assembly 2 and the Z-axis assembly 3 to enable the optical fiber sensor 12 (the model is PRA3Y10QD) in the Z-axis assembly 3 to move to the position above the consumable to be detected; observing the measured values of the optical fiber amplifier 10 (model number ER2-22HM) with and without consumables placed, respectively; adjusting the power and corresponding time of the optical fiber amplifier 9 and the height of the Z-axis component 3 to enable the measurement of the optical fiber amplifier in the state of placing consumables to be more than or equal to half of a full range, and setting the value as a corresponding value of the maximum value of the analog quantity;
s2: corresponding consumables are respectively placed in the gun head placing area 6, the filter placing area 8, the blood collection tube placing area 7 and the glass slide placing area 5, and consumables in all areas are not full;
s3: running a consumable detection program, waiting for the detection to be completed, observing all detection values on a display screen, and setting a judgment threshold value for whether each consumable exists or not;
s4: and judging whether a threshold value exists or not according to each consumable material, and distinguishing whether each station places a corresponding consumable material or not.
In this embodiment, through the above operation, the setting value of the optical fiber sensor 9 is outputted at 9000 corresponding to 5V, the judgment threshold of the tip is 2V, the judgment threshold of the filter is 1.2V, the judgment threshold of the blood collection tube is 1.2V, and the judgment threshold of the slide glass is 1.7V. The above-mentioned several consumptive materials have or not the condition to detect through the manual work, confirm that the consumptive material actually has or not the condition and accords with the testing result in this embodiment.
According to the device for detecting the existence of the consumables in the cell sorter, whether the consumables are placed on the station of each consumable placing area is judged based on the optical fiber signal transmitted by the optical fiber assembly and the optical signal received by the optical fiber assembly by additionally arranging the optical fiber sensor and the optical fiber transmission amplifier on the Z shaft assembly, the existence of various consumables can be automatically judged after basic setting is completed, and the detection result is displayed on the display screen, so that a detector can timely know the existence of the consumables in each placing area.
The above embodiments are merely to explain the technical solutions of the present invention in detail, and the present invention is not limited to the above embodiments, and it should be understood by those skilled in the art that all modifications and substitutions based on the above principles and spirit of the present invention should be within the protection scope of the present invention.
Claims (9)
1. The utility model provides a detect device that consumptive material had or not among cell sorter which characterized in that: the cell sorter comprises an X-axis assembly, a Y-axis assembly and a Z-axis assembly which are arranged in a cell sorter, wherein an optical fiber assembly for detecting whether consumables exist is arranged on the Z-axis assembly, and the optical fiber assembly is suitable for adjusting position coordinates in the X-axis direction, the Y-axis direction and the Z-axis direction in a stroke adjusting interval of the X-axis assembly, the Y-axis assembly and the Z-axis assembly; the cell sorter is located the below in X axle subassembly, Y axle subassembly, Z axle subassembly's coordinate regulation district is provided with the consumptive material and places the district, the optic fibre subassembly is suitable for the adjustment to the top of district arbitrary position is placed to the consumptive material.
2. The apparatus for detecting the presence or absence of a consumable in a cell sorter according to claim 1, wherein: the optical fiber assembly is at least provided with one group, and each group of optical fiber assembly comprises an optical fiber amplifier and an optical fiber sensor.
3. The apparatus for detecting the presence or absence of a consumable in a cell sorter according to claim 2, wherein: the optical fiber amplifier is arranged on the Z-axis component, and the optical fiber sensor is suitable for adjusting position coordinates along the X-axis direction, the Y-axis direction and the Z-axis direction.
4. The apparatus for detecting the presence or absence of a consumable in a cell sorter according to any of claims 1 to 3, wherein: the diameter range of the optical fiber core of the optical fiber component is 0.1mm-5 mm.
5. The apparatus for detecting the presence or absence of a consumable in a cell sorter according to claim 4, wherein: the color of the emitted light of the optical fiber component is at least one of red light, blue light, green light and white light.
6. The apparatus for detecting the presence or absence of a consumable in a cell sorter according to claim 4, wherein: the cell sorter also comprises a display for displaying the detection result, wherein the display is fixedly arranged on the body of the cell sorter.
7. The apparatus for detecting the presence or absence of a consumable in a cell sorter according to claim 1, wherein: the consumable material placing area comprises at least one of a kit placing area, a gun head placing area, a filter placing area, a blood collection tube placing area, a kit placing area and a glass slide placing area.
8. A method for detecting the presence or absence of a consumable in a cell sorter as claimed in any of claims 1 to 7, comprising the steps of:
s1: moving the X shaft assembly, the Y shaft assembly and the Z shaft assembly to enable an optical fiber sensor in an optical fiber assembly on the Z shaft assembly to move to the position above a consumable to be detected; observing the measured values of the optical fiber amplifiers in the optical fiber assembly under the condition that the consumables are placed and the consumables are not placed respectively; adjusting the power and corresponding time of the optical fiber amplifier and the height of the Z-axis component to enable the measurement of the optical fiber amplifier in the state of placing consumables to be more than or equal to half of a full range, and setting the value as a corresponding value of the maximum value of the analog quantity;
s2: corresponding consumables are respectively placed in the gun head placing area, the filter placing area, the blood collection tube placing area and the glass slide placing area, and consumables in all the areas are not full;
s3: running a consumable detection program, waiting for the detection to be completed, observing all detection values on a display screen, and setting a judgment threshold value for whether each consumable exists or not;
s4: and judging whether a threshold value exists or not according to each consumable material, and distinguishing whether each station places a corresponding consumable material or not.
9. The method of claim 8, wherein the Z-axis module has two or more sets of optical fiber modules, and the step S2 is repeated to set the maximum analog value of each optical fiber amplifier in each set of optical fiber modules.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011575038.3A CN112540417A (en) | 2020-12-28 | 2020-12-28 | Device and method for detecting existence of consumable materials in cell sorter |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011575038.3A CN112540417A (en) | 2020-12-28 | 2020-12-28 | Device and method for detecting existence of consumable materials in cell sorter |
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| CN112540417A true CN112540417A (en) | 2021-03-23 |
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| CN202011575038.3A Pending CN112540417A (en) | 2020-12-28 | 2020-12-28 | Device and method for detecting existence of consumable materials in cell sorter |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114067315A (en) * | 2021-10-23 | 2022-02-18 | 广州市艾贝泰生物科技有限公司 | Cell counting method, cell counting device, computer device, and storage medium |
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| CN110940568A (en) * | 2019-12-17 | 2020-03-31 | 武汉友芝友医疗科技股份有限公司 | Integrated system for cell enrichment, separation, dyeing and flaking |
| CN110964630A (en) * | 2019-12-18 | 2020-04-07 | 武汉友芝友医疗科技股份有限公司 | Device and method for detecting liquid separation completeness in cell filter |
| CN111027409A (en) * | 2019-11-19 | 2020-04-17 | 西安天隆科技有限公司 | Liquid transfer workbench and method for recognizing and monitoring consumable materials by using liquid transfer workbench |
| CN213780398U (en) * | 2020-12-28 | 2021-07-23 | 武汉友芝友医疗科技股份有限公司 | Device for detecting whether consumable materials exist in cell sorter |
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- 2020-12-28 CN CN202011575038.3A patent/CN112540417A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111027409A (en) * | 2019-11-19 | 2020-04-17 | 西安天隆科技有限公司 | Liquid transfer workbench and method for recognizing and monitoring consumable materials by using liquid transfer workbench |
| CN110940568A (en) * | 2019-12-17 | 2020-03-31 | 武汉友芝友医疗科技股份有限公司 | Integrated system for cell enrichment, separation, dyeing and flaking |
| CN110964630A (en) * | 2019-12-18 | 2020-04-07 | 武汉友芝友医疗科技股份有限公司 | Device and method for detecting liquid separation completeness in cell filter |
| CN213780398U (en) * | 2020-12-28 | 2021-07-23 | 武汉友芝友医疗科技股份有限公司 | Device for detecting whether consumable materials exist in cell sorter |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114067315A (en) * | 2021-10-23 | 2022-02-18 | 广州市艾贝泰生物科技有限公司 | Cell counting method, cell counting device, computer device, and storage medium |
| CN114067315B (en) * | 2021-10-23 | 2022-11-29 | 广州市艾贝泰生物科技有限公司 | Cell counting method, cell counting device, computer device, and storage medium |
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