CN213060874U

CN213060874U - Optical standard device for real-time fluorescence quantitative PCR instrument

Info

Publication number: CN213060874U
Application number: CN202120585820.7U
Authority: CN
Inventors: 董亮; 刘秀丽; 钟衍程; 张辰; 孙俊峰; 刘文敏; 朱娟; 曾宪化; 周文; 李莉; 魏明; 戴翔; 卓华
Original assignee: Beijing Lindian Weiye Electronic Technology Co ltd
Current assignee: Beijing Lindian Weiye Electronic Technology Co ltd
Priority date: 2021-03-23
Filing date: 2021-03-23
Publication date: 2021-04-27
Anticipated expiration: 2031-03-23

Abstract

The utility model relates to the technical field of the quality control of an optical path system of a fluorescence quantitative PCR instrument, in particular to an optical standard device for a real-time fluorescence quantitative PCR instrument, which comprises a device bottom plate, wherein the end surface of the device bottom plate is provided with a circuit board, the bottom of the circuit board is provided with a button cell, the end surface of the circuit board is provided with a bottom optical probe, and the end surface of the circuit board is provided with a temperature switch, the utility model can adopt a physical method to detect the optical path system of the fluorescence quantitative PCR instrument for bottom fluorescence detection and side fluorescence detection through the arranged bottom optical probe, thereby effectively evaluating the reliability and the accuracy of the fluorescence quantitative PCR instrument, solving the problems that the domestic fluorescence quantitative PCR instrument generally adopts the principle of bottom fluorescence detection or side fluorescence detection, lacks effective metering means and quality control means at present and cannot carry out effective quality control, and further, the device can detect the accuracy and reliability of the fluorescent PCR based on the bottom or side fluorescent detection principle, so that the device is more controllable.

Description

Optical standard device for real-time fluorescence quantitative PCR instrument

Technical Field

The utility model relates to a light path system quality control technical field of fluorescence quantitative PCR appearance specifically is an optical standard device for real-time fluorescence quantitative PCR appearance.

Background

The real-time fluorescent quantitative PCR technology is a method of adding fluorescent groups into a PCR reaction system, monitoring the whole PCR reaction process in real time by utilizing the accumulation of fluorescent signals, and finally carrying out quantitative analysis on an unknown template through a standard curve. The fluorescence detection system of the real-time fluorescence quantitative PCR instrument comprises three types of top fluorescence detection, bottom fluorescence detection and side fluorescence detection, wherein the imported fluorescence quantitative PCR instrument is generally used for top fluorescence detection, the domestic fluorescence quantitative PCR instrument is mainly used for bottom fluorescence detection and side fluorescence detection, and the detection hole is arranged at the bottom or the side. The detection accuracy of the fluorescence quantitative PCR instrument mainly depends on the accuracy of a temperature module and the reliability of an optical path system, the detection of the temperature module of the fluorescence quantitative PCR instrument has a mature technology at present, foreign and domestic technologies can detect the optical path system of the fluorescence quantitative PCR instrument for top detection, the existing physical optical standard device at home and abroad can only be suitable for the real-time fluorescence quantitative PCR instrument for top lighting and can not be used for the real-time fluorescence quantitative PCR instrument for bottom lighting or side lighting, and the optical path system detection, calibration or quality control function of the real-time fluorescence quantitative PCR instrument for bottom lighting or side lighting adopts a chemical method or a biological method, so that the operation is complex, the human introduction error is large, the traceability is unavailable, the quantitative transmission is not possessed, and the real-time fluorescence quantitative PCR instrument for bottom lighting or side lighting has no physical detection device.

There is therefore a need for an optical standard for real-time fluorescence quantitative PCR instruments that ameliorates the above problems.

Disclosure of Invention

An object of the utility model is to provide an optics standard device for real-time fluorescence quantitative PCR appearance to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme:

the utility model provides an optics standard device for real-time fluorescence quantitative PCR appearance, includes the device bottom plate, the terminal surface of device bottom plate is equipped with the circuit board, the bottom of circuit board is equipped with button cell, the terminal surface of circuit board is equipped with bottom optical probe, the terminal surface of circuit board is equipped with temperature switch, temperature switch's bottom is and be connected with the temperature switch bottom plate with the circuit board, temperature switch's terminal surface is equipped with metal temperature probe.

Preferably, the bottom optical probe is formed by wrapping a standard light source by a light-transmitting material, and the brightness of the standard light source can be controlled by controlling the current through a circuit; the light-transmitting material is an organic porous material, and can ensure the transmittance and uniformity of a standard light source.

Preferably, the metal temperature probe is composed of a high-precision temperature sensor, a red copper gold-plated shell and a PEEK material, the high-precision temperature sensor is a thermistor, and the temperature switch can control the temperature change of the bottom optical probe through the collected temperature change.

Preferably, the circuit board is a PCB circuit board, bottom optical probe, temperature switch and button cell are integrated on the circuit board to fixed by the device bottom plate, the size of the device bottom plate is the same as the Block module size of the fluorescence quantitative PCR to be measured.

Preferably, the bottom optical probe is of the same size as the temperature switch and fits completely into the Block well of the fluorometric PCR being measured.

Preferably, the number of the bottom optical probes and the temperature switches is at least one, and the total number of the bottom optical probes and the temperature switches is fifteen, so that the bottom optical probes and the temperature switches are freely combined and uniformly distributed on the end face of the circuit board.

Preferably, the bottom optical probe and the temperature switch are electrically connected through a wire and a button cell.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the utility model discloses in, through the bottom optical probe who sets up, can take the optical path system of physical method to the fluorescence quantitative PCR appearance of bottom fluorescence detection and side fluorescence detection and detect, effectively aassessment fluorescence quantitative PCR appearance's reliability and accuracy to solved and used bottom fluorescence detection and side fluorescence detection to lack effectual measurement means, quality control means at present for the domestic fluorescence PCR of principle, can't carry out effective quality control's problem, and then the practicality that makes the device obtains further promotion.

2. In the utility model, through the free combination of the bottom optical probe and the temperature switch, the device can be suitable for the quality control and the performance evaluation of the fluorescent quantitative PCR instrument of various types of bottom fluorescence detection and side fluorescence detection, the device can simulate the single-hole gene amplification process and can also simulate the gene amplification process of a standard curve method, the fluorescent quantitative PCR instrument of the bottom fluorescence detection and the side fluorescence detection can be more comprehensively evaluated, through evaluating the light path system, the device not only can meet the accuracy and the reliability of the experiment, but also is beneficial to finding the equipment design problem, and the improvement and optimization scheme is reversely provided, thereby solving the problems that the existing light path system of the real-time fluorescent quantitative PCR instrument of bottom lighting or side lighting adopts a chemical method or a biological method, the operation is complex, the introduced error is large, the source cannot be traced, and the mass transmissibility is not possessed, the real-time fluorescent quantitative PCR instrument with bottom lighting or side lighting has no problem of a physical detection device.

Drawings

FIG. 1 is a front view of the present invention;

FIG. 2 is a bottom view of the present invention;

FIG. 3 is an enlarged view of part A of the present invention;

fig. 4 is a diagram of an embodiment of the present invention.

In the figure: 1. a device chassis; 2. a circuit board; 3. a button cell; 4. a bottom optical probe; 5. a temperature switch; 6. a temperature switch base plate; 7. a metal temperature probe.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative work belong to the protection scope of the present invention based on the embodiments of the present invention.

Referring to fig. 1-4, the present invention provides a technical solution:

the utility model provides an optics standard device for real-time fluorescence quantitative PCR appearance, includes device bottom plate 1, and the terminal surface of device bottom plate 1 is equipped with circuit board 2, and the bottom of circuit board 2 is equipped with button cell 3, and the terminal surface of circuit board 2 is equipped with bottom optical probe 4, and the terminal surface of circuit board 2 is equipped with temperature switch 5, and temperature switch 5's bottom is and be connected with circuit board 2 and have temperature switch bottom plate 6, and temperature switch 5's terminal surface is equipped with metal temperature probe 7.

The specific implementation case is as follows:

when in use, the fluorescent quantitative PCR instrument is started to work and then placed in a Block (temperature control template) of the fluorescent quantitative PCR instrument, the relative brightness of all optical probes is controlled according to the temperature collected by a temperature switch so as to simulate gene amplification reaction, a sent standard light source is collected by a detector of the fluorescent quantitative PCR instrument through a lighting hole at the bottom or the side of the Block to generate an amplification curve, and the light path system of the fluorescent quantitative PCR instrument is evaluated by analyzing the result of the amplification curve and the change of the standard light source provided by the utility model, as shown in figure 4, the utility model discloses a physical method can be adopted to detect the light path system of the fluorescent quantitative PCR instrument for bottom fluorescent detection and side fluorescent detection through a bottom optical probe 4, thereby effectively evaluating the reliability and accuracy of the fluorescent quantitative PCR instrument, and solving the common bottom fluorescent detection and side fluorescent detection principle of the domestic fluorescent quantitative PCR instrument, at present, effective measurement means and quality control means are lacked, and the problem of effective quality control cannot be carried out, so that the practicability of the device is further improved, the device can be suitable for the quality control and performance evaluation of fluorescent quantitative PCR instruments of various types of bottom fluorescence detection and side fluorescence detection through the free combination of a bottom optical probe 4 and a temperature switch 5, can simulate the single-hole gene amplification process and the gene amplification process of a standard curve method, can carry out more comprehensive evaluation on the fluorescent quantitative PCR instruments of the bottom fluorescence detection and the side fluorescence detection, can meet the accuracy and reliability of experiments through the evaluation of a light path system, is favorable for finding the design problem of equipment, and reversely provides an improved optimization scheme, so that the problems of detection, performance evaluation, and the like of the light path system of the existing real-time fluorescent quantitative PCR instrument for bottom lighting or side lighting are solved, The calibration or quality control function adopts a chemical method or a biological method, the operation is complex, the human introduced error is large, the tracing cannot be realized, the mass transfer performance is not realized, and the real-time fluorescent quantitative PCR instrument for bottom lighting or side lighting has no physical detection device, so that the device can detect the accuracy and the reliability of the fluorescent PCR of the bottom or side fluorescent detection principle, and the device is more controllable.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. An optical standard device for a real-time fluorescent quantitative PCR instrument, comprising a device base plate (1), characterized in that: the device is characterized in that the end face of the device bottom plate (1) is provided with a circuit board (2), the bottom of the circuit board (2) is provided with a button cell (3), the end face of the circuit board (2) is provided with a bottom optical probe (4), the end face of the circuit board (2) is provided with a temperature switch (5), the bottom of the temperature switch (5) is connected with a temperature switch bottom plate (6) and the circuit board (2), and the end face of the temperature switch (5) is provided with a metal temperature probe (7).

2. The optical standard device of claim 1, wherein the optical standard device comprises: the bottom optical probe (4) is formed by wrapping a standard light source by a light-transmitting material, and the brightness of the standard light source can be controlled by controlling the current through a circuit; the light-transmitting material is an organic porous material, and can ensure the transmittance and uniformity of a standard light source.

3. The optical standard device of claim 1, wherein the optical standard device comprises: the metal temperature probe (7) is composed of a high-precision temperature sensor, a red copper gold-plated shell and a PEEK material, the high-precision temperature sensor is a thermistor, the brightness change of the bottom optical probe (4) can be controlled through the collected temperature change of the temperature switch (5), and the preset collection temperature and brightness can be changed according to the requirement.

4. The optical standard device of claim 1, wherein the optical standard device comprises: the circuit board (2) is a PCB circuit board, the bottom optical probe (4), the temperature switch (5) and the button cell (3) are integrated on the circuit board (2) and are fixed by the device bottom plate (1), and the size of the device bottom plate (1) is the same as the size of a Block module of the fluorescence quantitative PCR to be detected.

5. The optical standard device of claim 1, wherein the optical standard device comprises: the bottom optical probe (4) is consistent with the temperature switch (5) in size and is completely attached to the Block hole of the fluorescence quantitative PCR to be detected.

6. The optical standard device of claim 1, wherein the optical standard device comprises: the number of the bottom optical probes (4) and the temperature switches (5) is at least one, and the total number of the bottom optical probes and the temperature switches is fifteen, and the bottom optical probes and the temperature switches are freely combined and uniformly distributed on the end face of the circuit board (2).

7. The optical standard device of claim 1, wherein the optical standard device comprises: the bottom optical probe (4) and the temperature switch (5) are electrically connected through a lead and the button cell (3).