CN203914904U - A kind of engineered construction speed measuring device - Google Patents
A kind of engineered construction speed measuring device Download PDFInfo
- Publication number
- CN203914904U CN203914904U CN201320882080.9U CN201320882080U CN203914904U CN 203914904 U CN203914904 U CN 203914904U CN 201320882080 U CN201320882080 U CN 201320882080U CN 203914904 U CN203914904 U CN 203914904U
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- metal electrode
- measurement component
- measuring device
- engineered construction
- engineered
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Abstract
This utility model belongs to biomedical engineering field, this utility model provides a kind of engineered construction speed measuring device, comprise measurement component (1) and insulation crust (2), on measurement component (1), be fixed with the equidistant metal electrode (3) more than six roots of sensation, extend to measurement component (1) is outside metal electrode (3) one end, in order to be connected with external equipment; Insulation crust (2) top is provided with the opening that matches with measurement component (1) in order to place measurement component (1).This utility model volume is little, can save production cost, and operational approach is simple, can measure quickly and accurately the conducting speed of engineered construction and exsomatizednerve.
Description
Technical field
This utility model belongs to the biological equipment field of biomedical engineering, is specifically related to a kind of engineered construction speed measuring device that is applicable to.
Background technology
In recent years, the development of organizational project is in the ascendant.It provides a kind of brand-new thinking and therapeutic strategy, is about to a small amount of seed cell compound with biomaterial after amplification in vitro, builds tissue or the organ make new advances, for substituting and repair pathological changes, damaged histoorgan, rebuilds physiological function.The structure organizations such as cartilage, bone, tendon, blood vessel, trachea of successfully having regenerated at present.Engineered cardiovascular and engineered nerve have also been obtained certain development.For heart tissue or nerve, their important measuring abilities are electrical conductivity.
The research starting in domestic engineered tissue (construction) field building is evening relatively, but also carried out the research of more association area, and for engineered construction conductivity aspect at present in the blank stage, its reason is engineered heart tissue or the nerve that external structure experimentation is used, its small volume, or length is shorter, the detection for this performance both at home and abroad does not also have unified detection technique, equipment or device.
Therefore have not yet to see the report that is specially adapted for engineered heart tissue or nervous tissue's conductivity checkout gear.
Utility model content
The purpose of this utility model is to fill up the blank of measuring engineered construction conducting speed aspect research, adopt the measuring principle of the neuroelectricity conduction velocity of international uniform standard, according to the little feature of engineered construction essence, provide a kind of and be specially adapted for engineered construction to detect the device of its conductivity performance.
For solving the problems of the technologies described above, this utility model provides a kind of engineered construction speed measuring device, comprises measurement component and insulation crust, is fixed with 6 above equidistant metal electrodes on measurement component, extend to measurement component is outside metal electrode one end, in order to be connected with external equipment; Insulation crust top is provided with the opening that matches with measurement component in order to place measurement component.
Preferably, the adjacent metal electrode spacing of described measurement component is 1.5mm-2.5mm, and the best is 1.625mm-2mm.
The number of described metal electrode, the best is 6.
Preferably, metal electrode is platinum filament, filamentary silver, spun gold, stainless pin, and the best is platinum filament.
Preferably, the device of the engineered construction conducting speed of described measurement, also comprises a device block matching with insulation crust.
Described metal electrode diameter is 0.5-0.05mm, and the best is 0.125mm.Wherein, 0.125mm is the minimum dimension that current production company produces, if WeiLai Technology development can be produced the metal electrode of thinner size, for making tachometric survey more accurately can select thinner size.
The below of described metal electrode is cavity, has deposited a certain amount of buffer solution.
Described engineered construction speed measuring device, can also be for testing the conducting speed of exsomatizednerve.
Utilize device of the present utility model, measure the method for engineered construction conducting speed, comprise the steps:
Measurement component is connected with MPA2000m multichannel bio signal analytical system, be specially, by the red electrode of the sti passage of MPA2000m multichannel bio signal analytical system, (in accompanying drawing, red electrode represents by Lycoperdon polymorphum Vitt, lower same) be connected with the first metal electrode, black electrodes is connected with the second metal electrode; The white electrode of the B passage of MPA2000m multichannel bio signal analytical system is connected with the 3rd metal electrode, and red electrode is connected with the 4th metal electrode, and black electrodes is connected with the second metal electrode; The white electrode of the C-channel of MPA2000m multichannel bio signal analytical system is connected with the 5th metal electrode, and red electrode is connected with the 6th metal electrode, and black electrodes is connected with the second metal electrode;
Open MPA2000m multichannel bio signal analytical system software, connect;
The timbering material being equal to of not planting seed cell under engineered construction same culture conditions with to be measured is placed on the metal electrode of measurement component, and covers six electrodes simultaneously, zero;
The timbering material being equal to of not planting seed cell with engineered construction displacement to be measured, carries out electricity irritation, and the frequency of stimulating current is 1-200Hz, and voltage is 1-5V, the stimulus waveform obtaining by software records;
Input Δ d, the conducting speed that goes out this construction by computed in software, is specially, by the distance, delta d between B, two channel electrodes of C divided by time difference Δ t between the corresponding crest of conductivity waveform, wherein, Δ d is the twice of adjacent two metal electrode spacing and metal electrode diameter sum.
Engineered construction speed measuring device of the present utility model, the interelectrode distance of adjacent metal is 1.5mm-2.5mm, be applicable to the tissue constructs (the about 1cm of length) in existing experimentation, overcome existing measurement conducting speed device and cannot realize the shortcoming that engineered construction is measured, meet the little feature of engineered construction essence, meet the basic demand of current Tissue Engineering Study.This device is equally applicable to carry out the measurement of the short and small nerve that existing nerve conduction velocity measuring method measures.
This device is comprised of insulation crust and measurement component, simple in structure, can save production cost, in conjunction with MPA2000m multichannel bio signal analytical system, measure the conducting speed of engineered construction, operational approach is simple, can obtain quickly and accurately measurement result.
Engineered construction speed measuring device of the present utility model, compares compared with prior art, adopts this device to measure and has repeatability, and concordance, generally applies and will reach standard, the uniformity of engineered construction conducting speed measurement result.
In addition, this device is also applicable to the measurement of exsomatizednerve conducting speed.
Used the engineered construction speed measuring device in this utility model, measured the conducting speed of engineered construction in conjunction with MPA2000m multichannel bio signal analytical system, measuring method is simple, can obtain quickly and accurately measurement result.
In the equipment cavity of the metal electrode plane below of engineered construction speed measuring device of the present utility model, deposit a certain amount of buffer solution, can prevent from measuring the dry of thing, during maintaining measurement, measure the normal condition of thing, thereby guarantee the accuracy of measurement result.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model,
Fig. 2 is the structural representation of embodiment of the present utility model,
Fig. 3 is the circuit connection diagram of this utility model method,
Fig. 4 is conductivity checkout gear simulation drawing,
Fig. 5 is conductivity signal mode graphoid,
Wherein: 1 is measurement component; 2 is insulation crust; 3 is metal electrode; 4 is MPA2000m multichannel bio signal analytical system; 5 for being equipped with the computer of MPA2000m multichannel bio signal analytical system software; 31 is the first metal electrode; 32 is the second metal electrode; 33 is the 3rd metal electrode; 34 is the 4th metal electrode; 35 is the 5th metal electrode; 36 is the 6th metal electrode.
The specific embodiment
Below in conjunction with drawings and Examples, this utility model is described in further detail, but enforcement of the present utility model is not limited only to this.
Embodiment 1:
As shown in Figure 1, a kind of engineered construction speed measuring device, comprises measurement component 1 and insulation crust 2, is fixed with 6 equidistant metal electrodes 3 on measurement component 1, and extend to measurement component 1 is outside metal electrode 3 one end, in order to be connected with external equipment; Insulation crust 2 tops are provided with the opening that matches with measurement component 1 in order to place measurement component 1.
Embodiment 2:
An engineered construction speed measuring device, all the other also comprise a device block matching with insulation crust 2 with embodiment 1.
The spacing of described adjacent metal electrode 3 is 1.625mm.
Described metal electrode 3 is platinum filament.
Embodiment 3:
An engineered construction speed measuring device, all the other are with embodiment 1.
The spacing of described adjacent metal electrode 3 is 1.5mm.
Described metal electrode 3 is filamentary silver.
Embodiment 4:
An engineered construction speed measuring device, all the other are with embodiment 1.
Described metal electrode 3 is spun gold.
The spacing of described adjacent metal electrode 3 is 2mm.
The number of described metal electrode 3 is 7.
Embodiment 5:
Utilize a kind of engineered construction speed measuring device of embodiment 2, measure the method for engineered construction conducting speed, its step is as follows:
Be ready to engineered construction to be measured, it is high that size when it is measured maintains the wide * of the long * of 1cm * 0.5cm * 0.2cm(),
By thering is the platinum electrode that 6 diameters are 0.125mm, the measurement component 1 that adjacent electrode spacing is 1.625mm is connected with MPA2000m multichannel bio signal analytical system 4, be specially, by the red electrode of the sti passage of MPA2000m multichannel bio signal analytical system 4, (in accompanying drawing, red electrode represents by Lycoperdon polymorphum Vitt, lower same) be connected with the first metal electrode 31, black electrodes is connected with the second metal electrode 32; The white electrode of the B passage of MPA2000m multichannel bio signal analytical system 4 is connected with the 3rd metal electrode 33, and red electrode is connected with the 4th metal electrode 34, and black electrodes is connected with the second metal electrode 32; The white electrode of the C-channel of MPA2000m multichannel bio signal analytical system 4 is connected with the 5th metal electrode 35, and red electrode is connected with the 6th metal electrode 36, and black electrodes is connected with the second metal electrode 32,
Open MPA2000m multichannel bio signal analytical system software, connect,
The timbering material being equal to of not planting seed cell under engineered construction same culture conditions with to be measured is placed in the platinum electrode of measurement component 1, and covers six electrodes simultaneously, zero,
The timbering material being equal to of not planting seed cell with engineered construction displacement to be measured, carries out electricity irritation, and the frequency of stimulating current is 100Hz, and voltage is 3V, the stimulus waveform obtaining by software records.
By computed in software, go out the conducting speed of this construction, be specially, distance, delta d(Δ d between input B, two channel electrodes of C is the twice of adjacent two metal electrode spacing and metal electrode diameter sum), Δ d=(1.625mm+0.125mm) * 2=3.5mm, Δ d is shown by software voluntarily divided by time difference △ t(△ t between the corresponding crest of conductivity waveform, numerical value is 1500 μ s), the conducting speed that software calculates tissue constructs to be measured is automatically about 2.3m/s.
Embodiment 6:
All the other are with embodiment 5, metal electrode 3 every electrode diameters are 0.125mm, electrode spacing is 1.625mm, thing to be measured is exsomatizednerve, in zero step, do not need to use with engineered construction same culture conditions to be measured under the timbering material being equal to of not planting seed cell, exsomatizednerve length is 1.5cm.Between the corresponding crest of conductivity waveform, time difference Δ t is shown as 85.00us, and the distance, delta d between B, two channel electrodes of C is 3.5mm, and the conducting speed that software calculates tissue constructs to be measured is automatically about 412m/s.
Below the preferred embodiment of this utility model having been created illustrates, but this utility model is created and is not limited to described embodiment, those of ordinary skill in the art also can make all modification being equal to or replacement under the prerequisite without prejudice to this utility model creative spirit, and the modification that these are equal to or replacement are all included in the application's claim limited range.
Claims (5)
1. an engineered construction speed measuring device, it is characterized in that, this device comprises measurement component (1) and insulation crust (2), on measurement component (1), be fixed with 6 above equidistant metal electrodes (3), extend to measurement component (1) is outside metal electrode (3) one end, in order to be connected with external equipment; Insulation crust (2) top is provided with the opening that matches with measurement component (1) in order to place measurement component (1); Adjacent metal electrode (3) spacing of described measurement component (1) is 1.5mm-2.5mm.
2. according to the engineered construction speed measuring device described in claim 1, it is characterized in that, adjacent metal electrode (3) spacing of described measurement component (1) is 1.625mm-2mm.
3. engineered construction speed measuring device according to claim 1 and 2, is characterized in that, the number of described metal electrode (3) is 6.
4. engineered construction speed measuring device according to claim 1 and 2, is characterized in that, also comprises a device block matching with insulation crust (2).
5. engineered construction speed measuring device according to claim 1 and 2, is characterized in that, the below of described metal electrode (3) is cavity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320882080.9U CN203914904U (en) | 2013-12-30 | 2013-12-30 | A kind of engineered construction speed measuring device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320882080.9U CN203914904U (en) | 2013-12-30 | 2013-12-30 | A kind of engineered construction speed measuring device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203914904U true CN203914904U (en) | 2014-11-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320882080.9U Withdrawn - After Issue CN203914904U (en) | 2013-12-30 | 2013-12-30 | A kind of engineered construction speed measuring device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103690158A (en) * | 2013-12-30 | 2014-04-02 | 中国人民解放军第二军医大学 | Measuring device and method for electrical conduction speed in tissue engineered structures |
-
2013
- 2013-12-30 CN CN201320882080.9U patent/CN203914904U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103690158A (en) * | 2013-12-30 | 2014-04-02 | 中国人民解放军第二军医大学 | Measuring device and method for electrical conduction speed in tissue engineered structures |
| CN103690158B (en) * | 2013-12-30 | 2015-08-26 | 中国人民解放军第二军医大学 | Measure the apparatus and method of engineered construction conducting speed |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20141105 Effective date of abandoning: 20150826 |
|
| RGAV | Abandon patent right to avoid regrant |