CN103690158A - Measuring device and method for electrical conduction speed in tissue engineered structures - Google Patents
Measuring device and method for electrical conduction speed in tissue engineered structures Download PDFInfo
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- CN103690158A CN103690158A CN201310744104.9A CN201310744104A CN103690158A CN 103690158 A CN103690158 A CN 103690158A CN 201310744104 A CN201310744104 A CN 201310744104A CN 103690158 A CN103690158 A CN 103690158A
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Abstract
The invention belongs to the field of biomedical engineering and provides a measuring device for electrical conduction speed in tissue engineered structures. The device comprises a measuring part (1) and an insulating housing (2). More than six metal electrodes (3) equally spaced are fixed on the measuring part (1). One end of every metal electrode (3) extends out and is connected with an external device. The upper portion of the insulating housing (2) is provided with an opening which matches with the measuring part (1) and which is used for holding the measuring part (1). The invention further discloses a method of measuring electrical conduction speed in tissue engineered structures by the measuring device. The device and the method have the advantages that the size is small, production cost can be saved, the method is simple to operate, and the electrical conduction speed in tissue engineered structures and in-vitro nerves can be quickly and accurately measured.
Description
Technical field
The biological equipment field that the invention belongs to biomedical engineering, is specifically related to a kind of apparatus and method that are applicable to measure engineered construction conducting speed.
Background technology
In recent years, the development of organizational project is in the ascendant.It provides a kind of brand-new thinking and therapeutic strategy, is about to a small amount of seed cell compound with biomaterial after amplification in vitro, builds tissue or the organ make new advances, for substituting and repair pathological changes, damaged histoorgan, rebuilds physiological function.The structure organizations such as cartilage, bone, tendon, blood vessel, trachea of successfully having regenerated at present.Engineered cardiovascular and engineered nerve have also been obtained certain development.For heart tissue or nerve, their important measuring abilities are electrical conductivity.
The research starting in domestic engineered tissue (construction) field building is evening relatively, but also carried out the research of more association area, and for engineered construction conductivity aspect at present in the blank stage, its reason is engineered heart tissue or the nerve that external structure experimentation is used, its small volume, or length is shorter, the detection for this performance both at home and abroad does not also have unified detection technique, equipment or device.
Therefore have not yet to see the report that is specially adapted for engineered heart tissue or nervous tissue's conductivity checkout gear.
Summary of the invention
The object of the invention is to fill up the blank of measuring engineered construction conducting speed aspect research, adopt the measuring principle of the neuroelectricity conduction velocity of international uniform standard, according to the little feature of engineered construction essence, provide a kind of and be specially adapted for engineered construction to detect the device of its conductivity performance.
For solving the problems of the technologies described above, the invention provides a kind of device of measuring engineered construction conducting speed, comprise measurement component and insulation crust, on measurement component, be fixed with 6 above equidistant metal electrodes, extend to measurement component is outside metal electrode one end, in order to be connected with external equipment; Insulation crust top is provided with the opening that matches with measurement component in order to place measurement component.
Preferably, the adjacent metal electrode spacing of described measurement component is 1.5mm-2.5mm, and the best is 1.625mm-2mm.
The number of described metal electrode, the best is 6.
Preferably, metal electrode is platinum filament, filamentary silver, spun gold, stainless pin, and the best is platinum filament.
Preferably, the device of the engineered construction conducting speed of described measurement, also comprises a device block matching with insulation crust.
Described metal electrode diameter is 0.5-0.05mm, and the best is 0.125mm.Wherein, 0.125mm is the minimum dimension that current production company produces, if WeiLai Technology development can be produced the metal electrode of thinner size, for making tachometric survey more accurately can select thinner size.
The below of described metal electrode is cavity, has deposited a certain amount of buffer solution.
The device of the engineered construction conducting speed of described measurement, can also be for testing the conducting speed of exsomatizednerve.
The present invention also provides a kind of method of utilizing said apparatus to measure engineered construction conducting speed, comprises the steps:
Measurement component is connected with MPA2000m multichannel bio signal analytical system, be specially, by the red electrode of the sti passage of MPA2000m multichannel bio signal analytical system, (in accompanying drawing, red electrode represents by Lycoperdon polymorphum Vitt, lower same) be connected with the first metal electrode, black electrodes is connected with the second metal electrode; The white electrode of the B passage of MPA2000m multichannel bio signal analytical system is connected with the 3rd metal electrode, and red electrode is connected with the 4th metal electrode, and black electrodes is connected with the second metal electrode; The white electrode of the C-channel of MPA2000m multichannel bio signal analytical system is connected with the 5th metal electrode, and red electrode is connected with the 6th metal electrode, and black electrodes is connected with the second metal electrode;
Open MPA2000m multichannel bio signal analytical system software, connect;
The timbering material being equal to of not planting seed cell under engineered construction same culture conditions with to be measured is placed on the metal electrode of measurement component, and covers six electrodes simultaneously, zero;
The timbering material being equal to of not planting seed cell with engineered construction displacement to be measured, carries out electricity irritation, and the frequency of stimulating current is 1-200Hz, and voltage is 1-5V, the stimulus waveform obtaining by software records;
Input Δ d, the conducting speed that goes out this construction by computed in software, is specially, by the distance, delta d between B, two channel electrodes of C divided by time difference Δ t between the corresponding crest of conductivity waveform, wherein, Δ d is the twice of adjacent two metal electrode spacing and metal electrode diameter sum.
The present invention measures the device of engineered construction conducting speed, the interelectrode distance of adjacent metal is 1.5mm-2.5mm, be applicable to the tissue constructs (the about 1cm of length) in existing experimentation, overcome existing measurement conducting speed device and cannot realize the shortcoming that engineered construction is measured, meet the little feature of engineered construction essence, meet the basic demand of current Tissue Engineering Study.This device is equally applicable to carry out the measurement of the short and small nerve that existing nerve conduction velocity measuring method measures.
This device is comprised of insulation crust and measurement component, simple in structure, can save production cost, in conjunction with MPA2000m multichannel bio signal analytical system, measure the conducting speed of engineered construction, operational approach is simple, can obtain quickly and accurately measurement result.
The present invention measures the device of engineered construction conducting speed, compared with prior art, compare, adopt this device to measure and have repeatability, concordance, generally applies and will reach standard, the uniformity of engineered construction conducting speed measurement result.
In addition, this device is also applicable to the measurement of exsomatizednerve conducting speed.
The present invention measures the method for engineered construction conducting speed, used the device of the engineered construction conducting speed of the measurement in the present invention, in conjunction with MPA2000m multichannel bio signal analytical system, measure the conducting speed of engineered construction, operational approach is simple, can obtain quickly and accurately measurement result.
The present invention measures in the equipment cavity of metal electrode plane below of device of engineered construction conducting speed and deposits a certain amount of buffer solution, can prevent from measuring the dry of thing, during maintaining measurement, measure the normal condition of thing, thereby guarantee the accuracy of measurement result.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention,
Fig. 2 is the structural representation of embodiments of the invention,
Fig. 3 is the circuit connection diagram of the inventive method,
Fig. 4 is conductivity checkout gear simulation drawing,
Fig. 5 is conductivity signal mode graphoid,
Fig. 6 is the schematic diagram of MPA2000m multichannel bio signal analytical system software,
Fig. 7 is for measuring the schematic diagram of engineered construction conducting speed result.
Wherein: 1 is measurement component; 2 is insulation crust; 3 is metal electrode; 4 is MPA2000m multichannel bio signal analytical system; 5 for being equipped with the computer of MPA2000m multichannel bio signal analytical system software; 31 is the first metal electrode; 32 is the second metal electrode; 33 is the 3rd metal electrode; 34 is the 4th metal electrode; 35 is the 5th metal electrode; 36 is the 6th metal electrode.
The specific embodiment
Below in conjunction with drawings and Examples, the present invention is described in further detail, but enforcement of the present invention is not limited only to this.
Embodiment 1:
As shown in Figure 1, measure a device for engineered construction conducting speed, comprise measurement component 1 and insulation crust 2, on measurement component 1, be fixed with 6 equidistant metal electrodes 3, extend to measurement component 1 is outside metal electrode 3 one end, in order to be connected with external equipment; Insulation crust 2 tops are provided with the opening that matches with measurement component 1 in order to place measurement component 1.
Embodiment 2:
Measure a device for engineered construction conducting speed, all the other also comprise a device block matching with insulation crust 2 with embodiment 1.
The spacing of described adjacent metal electrode 3 is 1.625mm.
Described metal electrode 3 is platinum filament.
Embodiment 3:
Measure a device for engineered construction conducting speed, all the other are with embodiment 1.
The spacing of described adjacent metal electrode 3 is 1.5mm.
Described metal electrode 3 is filamentary silver.
Embodiment 4:
Measure a device for engineered construction conducting speed, all the other are with embodiment 1.
Described metal electrode 3 is spun gold.
The spacing of described adjacent metal electrode 3 is 2mm.
The number of described metal electrode 3 is 7.
Embodiment 5:
Measure a method for engineered construction conducting speed, its step is as follows:
Be ready to engineered construction to be measured, it is high that size when it is measured maintains the wide * of the long * of 1cm * 0.5cm * 0.2cm(),
By thering is the platinum electrode that 6 diameters are 0.125mm, the measurement component 1 that adjacent electrode spacing is 1.625mm is connected with MPA2000m multichannel bio signal analytical system 4, be specially, by the red electrode of the sti passage of MPA2000m multichannel bio signal analytical system 4, (in accompanying drawing, red electrode represents by Lycoperdon polymorphum Vitt, lower same) be connected with the first metal electrode 31, black electrodes is connected with the second metal electrode 32; The white electrode of the B passage of MPA2000m multichannel bio signal analytical system 4 is connected with the 3rd metal electrode 33, and red electrode is connected with the 4th metal electrode 34, and black electrodes is connected with the second metal electrode 32; The white electrode of the C-channel of MPA2000m multichannel bio signal analytical system 4 is connected with the 5th metal electrode 35, and red electrode is connected with the 6th metal electrode 36, and black electrodes is connected with the second metal electrode 32,
Open MPA2000m multichannel bio signal analytical system software, connect,
The timbering material being equal to of not planting seed cell under engineered construction same culture conditions with to be measured is placed in the platinum electrode of measurement component 1, and covers six electrodes simultaneously, zero,
The timbering material being equal to of not planting seed cell with engineered construction displacement to be measured, carries out electricity irritation, and the frequency of stimulating current is 100Hz, and voltage is 3V, the stimulus waveform obtaining by software records, as shown in Figure 6.
By computed in software, go out the conducting speed of this construction, be specially, distance, delta d(Δ d between input B, two channel electrodes of C is the twice of adjacent two metal electrode spacing and metal electrode diameter sum, see Fig. 7), Δ d=(1.625mm+0.125mm) * 2=3.5mm, Δ d is shown by software voluntarily divided by time difference △ t(△ t between the corresponding crest of conductivity waveform, numerical value is 1500 μ s), the conducting speed that software calculates tissue constructs to be measured is automatically about 2.3m/s.
Embodiment 6:
A kind of method of measuring engineered construction conducting speed, all the other are with embodiment 3, metal electrode 3 every electrode diameters are 0.125mm, electrode spacing is 1.625mm, thing to be measured is exsomatizednerve, in zero step, do not need to use with engineered construction same culture conditions to be measured under the timbering material being equal to of not planting seed cell, exsomatizednerve length is 1.5cm.Between the corresponding crest of conductivity waveform, time difference Δ t is shown as 85.00us, and the distance, delta d between B, two channel electrodes of C is 3.5mm, and the conducting speed that software calculates tissue constructs to be measured is automatically about 412m/s.
Below the preferred embodiment of the invention is illustrated, but the invention is not limited to described embodiment, those of ordinary skill in the art also can make all modification being equal to or replacement under the prerequisite without prejudice to the invention spirit, and the modification that these are equal to or replacement are all included in the application's claim limited range.
Claims (8)
1. a device of measuring engineered construction conducting speed, comprise measurement component (1) and insulation crust (2), it is characterized in that, on measurement component (1), be fixed with 6 above equidistant metal electrodes (3), extend to measurement component (1) is outside metal electrode (3) one end, in order to be connected with external equipment; Insulation crust (2) top is provided with the opening that matches with measurement component (1) in order to place measurement component (1).
2. according to the device of the engineered construction conducting speed of the measurement described in claim 1, it is characterized in that, adjacent metal electrode (3) spacing of described measurement component (1) is 1.5mm-2.5mm.
3. according to the device of the engineered construction conducting speed of the measurement described in claim 1, it is characterized in that, adjacent metal electrode (3) spacing of described measurement component (1) is 1.625mm-2mm.
4. according to the device of the engineered construction conducting speed of measurement described in claim 1,2 or 3, it is characterized in that, the number of described metal electrode (3) is 6.
5. according to the device of the engineered construction conducting speed of measurement described in claim 1,2 or 3, it is characterized in that, described metal electrode (3) is platinum filament, filamentary silver, spun gold, or stainless pin.
6. according to the device of the engineered construction conducting speed of measurement described in claim 1,2 or 3, it is characterized in that, also comprise a device block matching with insulation crust (2).
7. according to the device of the engineered construction conducting speed of measurement described in claim 1,2 or 3, it is characterized in that, the below of described metal electrode (3) is cavity.
8. measure a method for engineered construction conducting speed, it is characterized in that, the method is to utilize the device of the engineered construction conducting speed of measurement as claimed in claim 4, and comprises the steps:
Measurement component (1) is connected with MPA2000m multichannel bio signal analytical system (4), be specially, the red electrode of the sti passage of MPA2000m multichannel bio signal analytical system (4) is connected with the first metal electrode (31), and black electrodes is connected with the second metal electrode (32); The white electrode of the B passage of MPA2000m multichannel bio signal analytical system (4) is connected with the 3rd metal electrode (33), and red electrode is connected with the 4th metal electrode (34), and black electrodes is connected with the second metal electrode (32); The white electrode of the C-channel of MPA2000m multichannel bio signal analytical system (4) is connected with the 5th metal electrode (35), and red electrode is connected with the 6th metal electrode (36), and black electrodes is connected with the second metal electrode (32);
Open MPA2000m multichannel bio signal analytical system software, connect;
The metal electrode (3) that the timbering material being equal to of not planting seed cell under engineered construction same culture conditions with to be measured is placed on to measurement component (1) is upper, and covers six electrodes simultaneously, zero;
The timbering material being equal to of not planting seed cell with engineered construction displacement to be measured, carries out electricity irritation, and the frequency of stimulating current is 1-200Hz, and voltage is 1-5V, the stimulus waveform obtaining by software records;
Input Δ d, by computed in software, go out the conducting speed of this construction, conducting speed is: by the distance, delta d between B, two channel electrodes of C divided by time difference Δ t between the corresponding crest of conductivity waveform, wherein, Δ d is the twice of adjacent two metal electrodes (3) spacing and metal electrode (3) diameter sum.
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| CN201310744104.9A CN103690158B (en) | 2013-12-30 | 2013-12-30 | Measure the apparatus and method of engineered construction conducting speed |
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Citations (4)
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|---|---|---|---|---|
| US4685466A (en) * | 1985-01-29 | 1987-08-11 | Rau Guenter | Measuring sensor for the non-invasive detection of electro-physiological quantities |
| ES2267333A1 (en) * | 2004-01-27 | 2007-03-01 | Universidad De Sevilla | Substance administration, stimulation and registration device for neuro-physiological use, has electrodes, camera and conduits arranged in sealed casing for monitoring and registering perfusion and instillation of substance in nerves |
| CN102247137A (en) * | 2010-05-19 | 2011-11-23 | 中国科学院电子学研究所 | Microelectrode array-based multichannel neural information detection system |
| CN203914904U (en) * | 2013-12-30 | 2014-11-05 | 中国人民解放军第二军医大学 | A kind of engineered construction speed measuring device |
-
2013
- 2013-12-30 CN CN201310744104.9A patent/CN103690158B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4685466A (en) * | 1985-01-29 | 1987-08-11 | Rau Guenter | Measuring sensor for the non-invasive detection of electro-physiological quantities |
| ES2267333A1 (en) * | 2004-01-27 | 2007-03-01 | Universidad De Sevilla | Substance administration, stimulation and registration device for neuro-physiological use, has electrodes, camera and conduits arranged in sealed casing for monitoring and registering perfusion and instillation of substance in nerves |
| CN102247137A (en) * | 2010-05-19 | 2011-11-23 | 中国科学院电子学研究所 | Microelectrode array-based multichannel neural information detection system |
| CN203914904U (en) * | 2013-12-30 | 2014-11-05 | 中国人民解放军第二军医大学 | A kind of engineered construction speed measuring device |
Non-Patent Citations (3)
| Title |
|---|
| 孙娟 等: "《心肌细胞与胶原材料复合再造组织工程心肌移植心肌梗死大鼠的心肌电生理变化》", 《中国组织上程研究》 * |
| 朱宝旺: "《MPA-2000M多道生物信号分析系统》", 《中国医学生物技术应用杂志》 * |
| 李晓童 等: "《组织工程化心传导束的体外构建研究》", 《中国解剖学会2012年年会论文文摘汇编》 * |
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