CN103690158B - Measure the apparatus and method of engineered construction conducting speed - Google Patents
Measure the apparatus and method of engineered construction conducting speed Download PDFInfo
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- CN103690158B CN103690158B CN201310744104.9A CN201310744104A CN103690158B CN 103690158 B CN103690158 B CN 103690158B CN 201310744104 A CN201310744104 A CN 201310744104A CN 103690158 B CN103690158 B CN 103690158B
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- conducting speed
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Abstract
The invention belongs to biomedical engineering field, the invention provides a kind of device measuring engineered construction conducting speed, comprise measurement component (1) and insulation crust (2), measurement component (1) is fixed with the equidistant metal electrode (3) of more than 6, metal electrode (3) one end extends, in order to be connected with external equipment to measurement component (1) outside; Insulation crust (2) top is provided with the opening that matches with measurement component (1) in order to place measurement component (1).The present invention also provides a kind of device of described measurement engineered construction conducting speed that utilizes to measure the method for engineered construction conducting speed.Volume of the present invention is little, can save production cost, and operational approach is simple, can measure the conducting speed of engineered construction and exsomatizednerve quickly and accurately.
Description
Technical field
The invention belongs to the biological clothing arts of biomedical engineering, be specifically related to a kind of apparatus and method being applicable to measure engineered construction conducting speed.
Background technology
In recent years, the development of organizational project is in the ascendant.It provide a kind of brand-new thinking and therapeutic strategy, by a small amount of seed cell after amplification in vitro with biomaterial compound, build the tissue that makes new advances or organ, for substituting and repair the histoorgan of pathological changes, defect, rebuild physiological function.The at present successful regeneration structure organizations such as cartilage, bone, tendon, blood vessel, trachea.Engineered cardiovascular and engineered nerve also achieve certain development.For heart tissue or nerve, namely their important measuring abilities are electrical conductivity.
The research starting relatively evening in domestic engineered tissue (construction) field building, but also to carry out the research of more association area, and the blank stage is at present for engineered construction conductivity aspect, its reason is engineered heart tissue or the nerve of external structure experimentation, its small volume, or length is shorter, both at home and abroad for the detection technique that the detection of this performance is also ununified, equipment or device.
Therefore the report being specially adapted for engineered heart tissue or nervous tissue's conductivity checkout gear is had not yet to see.
Summary of the invention
The object of the invention is to fill up the blank measuring engineered construction conducting speed aspect research, adopt the measuring principle of the neuroelectricity conduction velocity of international uniform standard, the feature little according to engineered construction essence, provides a kind of and is specially adapted for engineered construction to detect the device of its conductivity performance.
For solving the problems of the technologies described above, the invention provides a kind of device measuring engineered construction conducting speed, comprise measurement component and insulation crust, measurement component is fixed with the equidistant metal electrode of more than 6, metal electrode one end extends, in order to be connected with external equipment to measurement component outside; Insulation crust top is provided with the opening that matches with measurement component in order to place measurement component.
Preferably, the adjacent metal electrode spacing of described measurement component is 1.5mm-2.5mm, and the best is 1.625mm-2mm.
The number of described metal electrode, the best is 6.
Preferably, metal electrode is platinum filament, filamentary silver, spun gold, stainless pin, and the best is platinum filament.
Preferably, the device of the engineered construction conducting speed of described measurement, also comprises a device matched with insulation crust and blocks a shot.
Described metal electrode diameter is 0.5-0.05mm, and the best is 0.125mm.Wherein, 0.125mm is the minimum dimension that current production company produces, if future technical advances can produce the metal electrode of thinner size, more accurately can select thinner size for making tachometric survey.
The below of described metal electrode is cavity, has deposited a certain amount of buffer solution.
The device of the engineered construction conducting speed of described measurement, can also be used for the conducting speed of testing exsomatizednerve.
Present invention also offers a kind of method utilizing said apparatus to measure engineered construction conducting speed, comprise the steps:
Measurement component is connected with MPA2000m multichannel bio signal analytical system, be specially, by the red electrode of the sti passage of MPA2000m multichannel bio signal analytical system, (in accompanying drawing, red electrode Lycoperdon polymorphum Vitt represents, lower same) be connected with the first metal electrode, black electrodes is connected with the second metal electrode; Be connected with the 3rd metal electrode by the white electrode of the channel B of MPA2000m multichannel bio signal analytical system, red electrode is connected with the 4th metal electrode, and black electrodes is connected with the second metal electrode; Be connected with the 5th metal electrode by the white electrode of the C-channel of MPA2000m multichannel bio signal analytical system, red electrode is connected with the 6th metal electrode, and black electrodes is connected with the second metal electrode;
Open MPA2000m multichannel bio signal analytical system software, connect;
Be placed on the timbering material be equal to of not planting seed cell under engineered construction same culture conditions to be measured on the metal electrode of measurement component, and cover six electrodes simultaneously, compared with zero;
Do not plant the equivalent timbering material of seed cell with engineered construction displacement to be measured, carry out electricity irritation, the frequency of stimulating current is 1-200Hz, and voltage is 1-5V, the stimulus waveform obtained by software records;
Input Δ d, gone out the conducting speed of this construction by computed in software, be specially, by the distance, delta d between B, C two channel electrodes divided by time difference Δ t between conductivity waveform corresponding crest, wherein, Δ d is the twice of adjacent two metal electrode spacing and metal electrode diameter sum.
The present invention measures the device of engineered construction conducting speed, the interelectrode distance of adjacent metal is 1.5mm-2.5mm, be applicable to the tissue constructs (length is about 1cm) in existing experimentation, overcome the shortcoming that existing measurement conducting speed device cannot realize measuring engineered construction, meet the feature that engineered construction essence is little, meet the basic demand of current Tissue Engineering Study.This device is equally applicable to the measurement cannot carrying out the short and small nerve that existing nerve conduction velocity measuring method is measured.
This device is made up of insulation crust and measurement component, structure is simple, can save production cost, measure the conducting speed of engineered construction in conjunction with MPA2000m multichannel bio signal analytical system, operational approach is simple, can obtain measurement result quickly and accurately.
The present invention measures the device of engineered construction conducting speed, comparatively prior art is compared, adopting this device to carry out measurement and there is repeatability, concordance, generally applying standard, the uniformity by reaching engineered construction conducting speed measurement result.
In addition, this device is also applicable to the measurement of exsomatizednerve conducting speed.
The present invention measures the method for engineered construction conducting speed, employ the device of the engineered construction conducting speed of measurement in the present invention, the conducting speed of engineered construction is measured in conjunction with MPA2000m multichannel bio signal analytical system, operational approach is simple, can obtain measurement result quickly and accurately.
A certain amount of buffer solution is deposited in equipment cavity below the metal electrode plane that the present invention measures the device of engineered construction conducting speed, the drying of measuring thing can be prevented, maintain the normal condition measuring thing during measuring, thus ensure the accuracy of measurement result.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention,
Fig. 2 is the structural representation of embodiments of the invention,
Fig. 3 is the circuit connection diagram of the inventive method,
Fig. 4 is conductivity checkout gear simulation drawing,
Fig. 5 is conductivity signal mode graphoid,
Fig. 6 is the schematic diagram of MPA2000m multichannel bio signal analytical system software,
Fig. 7 is the schematic diagram measuring engineered construction conducting speed result.
Wherein: 1 is measurement component; 2 is insulation crust; 3 is metal electrode; 4 is MPA2000m multichannel bio signal analytical system; 5 for being equipped with the computer of MPA2000m multichannel bio signal analytical system software; 31 is the first metal electrode; 32 is the second metal electrode; 33 is the 3rd metal electrode; 34 is the 4th metal electrode; 35 is the 5th metal electrode; 36 is the 6th metal electrode.
Detailed description of the invention
Below in conjunction with drawings and Examples, the present invention is described in further detail, but enforcement of the present invention is not limited only to this.
Embodiment 1:
As shown in Figure 1, measure a device for engineered construction conducting speed, comprise measurement component 1 and insulation crust 2, measurement component 1 is fixed with 6 equidistant metal electrodes 3, metal electrode 3 one end extends, in order to be connected with external equipment to measurement component 1 outside; Insulation crust 2 top is provided with the opening that matches with measurement component 1 in order to place measurement component 1.
Embodiment 2:
Measure a device for engineered construction conducting speed, all the other are with embodiment 1, also comprise a device matched with insulation crust 2 and block a shot.
The spacing of described adjacent metal electrode 3 is 1.625mm.
Described metal electrode 3 is platinum filament.
Embodiment 3:
Measure a device for engineered construction conducting speed, all the other are with embodiment 1.
The spacing of described adjacent metal electrode 3 is 1.5mm.
Described metal electrode 3 is filamentary silver.
Embodiment 4:
Measure a device for engineered construction conducting speed, all the other are with embodiment 1.
Described metal electrode 3 is spun gold.
The spacing of described adjacent metal electrode 3 is 2mm.
The number of described metal electrode 3 is 7.
Embodiment 5:
Measure a method for engineered construction conducting speed, its step is as follows:
Get out engineered construction to be measured, it is high that size when it is measured maintains the wide * of the long * of 1cm × 0.5cm × 0.2cm(),
The measurement component 1 having platinum electrode that 6 diameters are 0.125mm, adjacent electrode spacing is 1.625mm is connected with MPA2000m multichannel bio signal analytical system 4, be specially, by the red electrode of the sti passage of MPA2000m multichannel bio signal analytical system 4, (in accompanying drawing, red electrode Lycoperdon polymorphum Vitt represents, lower same) be connected with the first metal electrode 31, black electrodes is connected with the second metal electrode 32; Be connected with the 3rd metal electrode 33 by the white electrode of the channel B of MPA2000m multichannel bio signal analytical system 4, red electrode is connected with the 4th metal electrode 34, and black electrodes is connected with the second metal electrode 32; Be connected with the 5th metal electrode 35 by the white electrode of the C-channel of MPA2000m multichannel bio signal analytical system 4, red electrode is connected with the 6th metal electrode 36, and black electrodes is connected with the second metal electrode 32,
Open MPA2000m multichannel bio signal analytical system software, connect,
Be placed on the timbering material be equal to of not planting seed cell under engineered construction same culture conditions to be measured in the platinum electrode of measurement component 1, and cover six electrodes simultaneously, compared with zero,
Do not plant the equivalent timbering material of seed cell with engineered construction displacement to be measured, carry out electricity irritation, the frequency of stimulating current is 100Hz, and voltage is 3V, the stimulus waveform obtained by software records, as shown in Figure 6.
The conducting speed of this construction is gone out by computed in software, be specially, distance, delta d(Δ d between input B, C two channel electrodes is the twice of adjacent two metal electrode spacing and metal electrode diameter sum, see Fig. 7), Δ d=(1.625mm+0.125mm) × 2=3.5mm, Δ d is shown by software voluntarily divided by time difference △ t(△ t between conductivity waveform corresponding crest, numerical value is 1500 μ s), the conducting speed that software calculates tissue constructs to be measured is automatically about 2.3m/s.
Embodiment 6:
A kind of method measuring engineered construction conducting speed, all the other are with embodiment 3, the every root electrode diameter of metal electrode 3 is 0.125mm, electrode spacing is 1.625mm, thing to be measured is exsomatizednerve, use and the timbering material be equal to of not planting seed cell under engineered construction same culture conditions to be measured compared with not needing in zero step, exsomatizednerve length is 1.5cm.Between the crest that conductivity waveform is corresponding, time difference Δ t is shown as 85.00us, and the distance, delta d between B, C two channel electrodes is 3.5mm, and the conducting speed that software calculates tissue constructs to be measured is automatically about 412m/s.
Below the preferred embodiment of the invention is illustrated, but the invention is not limited to described embodiment, those of ordinary skill in the art also can make all equivalent modification or replacement under the prerequisite without prejudice to the invention spirit, and these equivalent modification or replacement are all included in the application's claim limited range.
Claims (8)
1. measure the device of engineered construction conducting speed for one kind, comprise measurement component (1) and insulation crust (2), it is characterized in that, measurement component (1) is fixed with the equidistant metal electrode (3) of more than 6, metal electrode (3) one end extends, in order to be connected with external equipment to measurement component (1) outside; Insulation crust (2) top is provided with the opening that matches with measurement component (1) in order to place measurement component (1).
2. according to the device of the engineered construction conducting speed of the measurement described in claim 1, it is characterized in that, adjacent metal electrode (3) spacing of described measurement component (1) is 1.5mm-2.5mm.
3. according to the device of the engineered construction conducting speed of the measurement described in claim 1, it is characterized in that, adjacent metal electrode (3) spacing of described measurement component (1) is 1.625mm-2mm.
4. according to the device of the engineered construction conducting speed of measurement described in claim 1,2 or 3, it is characterized in that, the number of described metal electrode (3) is 6.
5. according to the device of the engineered construction conducting speed of measurement described in claim 1,2 or 3, it is characterized in that, described metal electrode (3) be platinum filament, filamentary silver, spun gold, or stainless pin.
6. according to the device of the engineered construction conducting speed of measurement described in claim 1,2 or 3, it is characterized in that, also comprise a device matched with insulation crust (2) and block a shot.
7. according to the device of the engineered construction conducting speed of measurement described in claim 1,2 or 3, it is characterized in that, the below of described metal electrode (3) is cavity.
8. measure a method for engineered construction conducting speed, it is characterized in that, the method utilizes the device measuring engineered construction conducting speed as claimed in claim 4, and comprise the steps:
Measurement component (1) is connected with MPA2000m multichannel bio signal analytical system (4), be specially, be connected with the first metal electrode (31) by the red electrode of the sti passage of MPA2000m multichannel bio signal analytical system (4), black electrodes is connected with the second metal electrode (32); Be connected with the 3rd metal electrode (33) by the white electrode of the channel B of MPA2000m multichannel bio signal analytical system (4), red electrode is connected with the 4th metal electrode (34), and black electrodes is connected with the second metal electrode (32); Be connected with the 5th metal electrode (35) by the white electrode of the C-channel of MPA2000m multichannel bio signal analytical system (4), red electrode is connected with the 6th metal electrode (36), and black electrodes is connected with the second metal electrode (32);
Open MPA2000m multichannel bio signal analytical system software, connect;
Be placed on the timbering material be equal to of not planting seed cell under engineered construction same culture conditions to be measured on the metal electrode (3) of measurement component (1), and cover six electrodes simultaneously, compared with zero;
Do not plant the equivalent timbering material of seed cell with engineered construction displacement to be measured, carry out electricity irritation, the frequency of stimulating current is 1-200Hz, and voltage is 1-5V, the stimulus waveform obtained by software records;
Input Δ d, the conducting speed of this construction is gone out by computed in software, conducting speed is: by the distance, delta d between B, C two channel electrodes divided by time difference Δ t between conductivity waveform corresponding crest, wherein, Δ d is the twice of adjacent two metal electrodes (3) spacing and metal electrode (3) diameter sum.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4685466A (en) * | 1985-01-29 | 1987-08-11 | Rau Guenter | Measuring sensor for the non-invasive detection of electro-physiological quantities |
| ES2267333A1 (en) * | 2004-01-27 | 2007-03-01 | Universidad De Sevilla | Substance administration, stimulation and registration device for neuro-physiological use, has electrodes, camera and conduits arranged in sealed casing for monitoring and registering perfusion and instillation of substance in nerves |
| CN102247137A (en) * | 2010-05-19 | 2011-11-23 | 中国科学院电子学研究所 | Microelectrode array-based multichannel neural information detection system |
| CN203914904U (en) * | 2013-12-30 | 2014-11-05 | 中国人民解放军第二军医大学 | A kind of engineered construction speed measuring device |
-
2013
- 2013-12-30 CN CN201310744104.9A patent/CN103690158B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4685466A (en) * | 1985-01-29 | 1987-08-11 | Rau Guenter | Measuring sensor for the non-invasive detection of electro-physiological quantities |
| ES2267333A1 (en) * | 2004-01-27 | 2007-03-01 | Universidad De Sevilla | Substance administration, stimulation and registration device for neuro-physiological use, has electrodes, camera and conduits arranged in sealed casing for monitoring and registering perfusion and instillation of substance in nerves |
| CN102247137A (en) * | 2010-05-19 | 2011-11-23 | 中国科学院电子学研究所 | Microelectrode array-based multichannel neural information detection system |
| CN203914904U (en) * | 2013-12-30 | 2014-11-05 | 中国人民解放军第二军医大学 | A kind of engineered construction speed measuring device |
Non-Patent Citations (3)
| Title |
|---|
| 《MPA-2000M多道生物信号分析系统》;朱宝旺;《中国医学生物技术应用杂志》;20020131;第51-58页 * |
| 《心肌细胞与胶原材料复合再造组织工程心肌移植心肌梗死大鼠的心肌电生理变化》;孙娟 等;《中国组织上程研究》;20121021;第16卷(第43期);第8078-8082页 * |
| 《组织工程化心传导束的体外构建研究》;李晓童 等;《中国解剖学会2012年年会论文文摘汇编》;20121029;第96页 * |
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