CN205786452U - A kind of amount of viable cell measuring instrument - Google PatentsA kind of amount of viable cell measuring instrument Download PDF
- Publication number
- CN205786452U CN205786452U CN201620736356.6U CN201620736356U CN205786452U CN 205786452 U CN205786452 U CN 205786452U CN 201620736356 U CN201620736356 U CN 201620736356U CN 205786452 U CN205786452 U CN 205786452U
- Prior art keywords
- measuring instrument
- viable cell
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
- 239000000523 sample Substances 0.000 claims abstract description 18
- 238000011065 in-situ storage Methods 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 4
- 230000005684 electric field Effects 0.000 claims description 8
- 230000005611 electricity Effects 0.000 claims description 3
- 238000005070 sampling Methods 0.000 abstract description 5
- 238000005259 measurement Methods 0.000 abstract description 4
- 210000004027 cells Anatomy 0.000 description 30
- 238000000034 method Methods 0.000 description 6
- 238000000855 fermentation Methods 0.000 description 5
- 230000004151 fermentation Effects 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000002028 Biomass Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 210000004243 Sweat Anatomy 0.000 description 3
- 210000000170 Cell Membrane Anatomy 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000006011 modification reaction Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 229920002574 CR-39 Polymers 0.000 description 1
- 230000036091 Metabolic activity Effects 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003990 capacitor Substances 0.000 description 1
- 230000001808 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000003111 delayed Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010949 in-process test method Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000002503 metabolic Effects 0.000 description 1
- 230000000051 modifying Effects 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 230000000630 rising Effects 0.000 description 1
- 230000003595 spectral Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000004148 unit process Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 200000000019 wound Diseases 0.000 description 1
The utility model discloses a kind of amount of viable cell measuring instrument, described measuring instrument includes popping one's head in (1) in situ, signals collecting amplifier (2), analog-digital converter (3) and processor (4), described original position probe (1) is for putting into measurement voltage in the liquid containing living cells, and (1) passes sequentially through signals collecting amplifier (2) to described original position probe, analog-digital converter (3) is connected with processor (4) signal.A kind of amount of viable cell measuring instrument of the present utility model can On-line sampling system living cells quantity.
This utility model relates to instrument analysis technology field, is specifically related to a kind of amount of viable cell measuring instrument.
Reliably amount of viable cell is one of very important parameter in each sweat, that live, have metabolic activity Cell quantity is closely related with production.All dynamic feeding strategy can be used to obtain higher cell in many sweats close Degree and production concentration, these implementations of strategies all depend on the quantity of living cells, and therefore the measurement in real time of this key parameter is non- The most important.Traditional amount of viable cell measuring method such as measures dry weight, absorbance and centrifugal volume etc., has operation complicated, delayed Time length, measurement result are living cells and the shortcoming of dead cell summation.FDA discloses PAT industrial directory in JIUYUE, 2004, will PAT is defined as one and can carry out design and analysis by measuring critical procedure parameter and quality index, control pharmaceutical production The mechanism of journey and means.Online amount of viable cell measuring instrument application in terms of Biomass monitoring has become as in process analysis technique Rising instrument.
Utility model content
The purpose of this utility model is to provide a kind of amount of viable cell measuring instrument, a kind of living cells of the present utility model to measure Amount instrument can On-line sampling system living cells quantity.
For achieving the above object, this utility model 1, a kind of amount of viable cell measuring instrument, described measuring instrument includes popping one's head in situ, Signals collecting amplifier, analog-digital converter and processor, described original position probe is measured for putting in the liquid containing living cells Voltage, described original position probe passes sequentially through signals collecting amplifier, analog-digital converter is connected with processor signal.
Preferably, described original position probe includes two alternating electric field output electrodes and two voltage detecting electrodes.
Preferably, described measuring instrument also includes digital frequency synthesizer, described digital frequency synthesizer and two alternation electricity Field output electrode electrical connection.
This utility model has the advantage that
The traditional measurement method of Biomass is all off-line, measures biomass concentration by off-line process cumbersome and time consuming, also From fermentation tank, to extract sample, in sampling process, easily to bring miscellaneous bacteria into and infect fermentation tank, use another of off-line method Individual shortcoming is, people can not realize automatically controlling sweat in this way.Online amount of viable cell measuring instrument is very The problems referred to above of good solution.This instrument has wide spectrum, test point selects feature flexibly, and this is the most external commercial apparatus Do not possess.In terms of the system integration, detection platform, data algorithm, combining closely of application are also main wounds Newly.This instrument can provide powerful to vast laboratory and the amount of viable cell detecting instrument of motility (simultaneously also can be as biology Other parameter detecting of suspension are used), meanwhile, this instrument miniaturization, production line easy for installation, with low cost, easy to use are former Position measuring instrument also certainly will promote that promotion China biotechnology, in the popularization of the applications such as bioreactor, is produced by this technology Industryization development is significant, and also has the advantage that
1. viable cell concentrations can be carried out that On-line sampling system, i.e. instrument record be biomass concentration at this very moment and Not being the numerical value in a certain moment in the past, probe is inserted directly in fermented product without sampling in situ；
2. the stability worked long hours and reliability；
3. probe can be at working site high temperature sterilize in situ, and probe does not infects and pollutes fermented product in situ；
4. reading is affected little by graininess float and dead yeast cells；
5. (such as fermentation broth contents, pH value, dissolved oxygen concentration, temperature, mixing speed, ventilation when fermentation condition changes Amounts etc. are when changing) reading of instrument is essentially unaffected.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of detailed description of the invention of amount of viable cell measuring instrument of the present utility model.
Detailed description of the invention
Detailed description below is used for illustrating this utility model, but is not limited to scope of the present utility model.
As it is shown in figure 1, this utility model provides a kind of amount of viable cell measuring instrument, described measuring instrument includes popping one's head in situ 1, letter Number collection amplifier 2, analog-digital converter 3 and processor 4, described original position probe 1 is surveyed for putting in the liquid containing living cells Amount voltage, described original position probe 1 passes sequentially through signals collecting amplifier 2, analog-digital converter 3 is connected with processor 4 signal.
Living cells has airtight cell membrane system, under the action of alternative electric field of certain frequency, in living cells charged carefully Kytoplasm migrates, and runs into cell membrane complete, insulation and forms polarization phenomena, and cell becomes the most small capacitor, institute The electric capacity surveyed becomes good linear relationship with the concentration of living cells.By measuring capacitance on electrode, demarcate reaction later and live thin Born of the same parents' concentration.Described original position probe 1 can include two alternating electric field output electrodes and two voltage detecting electrodes.Described measuring instrument Digital frequency synthesizer, described digital frequency synthesizer and two alternating electric field output electrode electrical connections can also be included.
Below by concrete amount of viable cell measuring instrument of the present utility model, but the most therefore limit this utility model.
Amount of viable cell measuring instrument, it is mainly by precision switch power circuit, digital frequency synthesizer, filter circuit, letter Number output amplifier, signals collecting amplifying circuit, analog to digital conversion circuit, data caching circuit, central processing unit, communication electricity Road, host computer and composition of popping one's head in situ.
Probe is made up of 4 electrodes in situ, and electrode is made up of platinum material.Digital frequency synthesizer output alternation Electric field is carried on two electrodes in outside.In fermentation tank, living cells produces polarization phenomena under action of alternative electric field, in inner side Detecting magnitude of voltage by signals collecting amplifying circuit on two electrodes, magnitude of voltage is converted into digital signal by analog-digital converter After, after delivering to central processing unit process, obtain capacitance and electric conductivity value data, upload the data to by RS-485 communicating circuit Host computer, host computer interface display curvilinear motion, judge viable cell concentrations more intuitively, visually, improve production efficiency.
This utility model uses precision switch power supply as power supply utility model, has work efficiency high, and it is excellent that volume is little etc. Point.The Switching Power Supply selected must be high-quality, carries out the process of power supply ripple, otherwise directly has signals collecting the biggest Impact.
This utility model communicating circuit uses RS-485 serial bus standard, and RS-485 interface uses balance driver and difference Dividing the combination of receptor, noise immunity is good, and transfer rate is fast, long transmission distance, is well suited for using in industry spot.
Data analysis and process is mainly used all phase fast fourier transform algorithm, all phase Fu by this utility model In leaf algorithm than conventional Fourier algorithm, there is more excellent suppression spectral leakage performance, strong interference immunity, precision is higher, be suitable for In accurate measurement occasion, amount of calculation is little, is conducive to realizing real-time.
Digital frequency synthesizer uses ADI company AD9852 chip, and this chips has the programmable frequency of 48 and deposits Device, 14 programmable phase offset registers, 12 programmable amplitude modulation and switch output shape keying function.The most permissible Make instrument reach wide spectrum, frequency selects versatility performance flexibly.By PC control AD9852 output frequency and amplitude Variable alternation cosine wave, simple, convenient.
Voltage on ADC sample circuit Real-time Collection probe medial electrode in situ, the central processing unit data to gathering are made All phase fast Fourier transform, reference signal is also made all phase fast Fourier transform simultaneously, is calculated and survey by central processing unit Measure signal and the phase contrast of reference signal and modulus value, calculate capacitance and electric conductivity value.
Communicating circuit uses ADI company magnetic coupling based on chip-scale transformator isolation technology, and communication delay is little, power consumption Stability low, superpower, the life-span is long.RS-485 Interface design has lightning protection discharge tube, TVS protection, filter network tertiary treatment, can answer To overwhelming majority thunderbolt, electromagnetic interference, electrostatic etc..
Although, the most with general explanation and specific embodiment, this utility model is made detailed description, but On the basis of this utility model, can make some modifications or improvements it, this is apparent to those skilled in the art 's.Therefore, these modifications or improvements on the basis of without departing from this utility model spirit, belong to this utility model and want Seek the scope of protection.
1. an amount of viable cell measuring instrument, it is characterised in that described measuring instrument includes popping one's head in situ (1), signals collecting amplifier (2), analog-digital converter (3) and processor (4), described original position probe (1) measures electricity for putting in the liquid containing living cells Pressure, (1) passes sequentially through signals collecting amplifier (2) to described original position probe, analog-digital converter (3) is connected with processor (4) signal.
Amount of viable cell measuring instrument the most according to claim 1, it is characterised in that described original position probe (1) includes two friendships Changed electric field output electrode and two voltage detecting electrodes.
Amount of viable cell measuring instrument the most according to claim 1, it is characterised in that described measuring instrument also includes that numerical frequency is closed Grow up to be a useful person, described digital frequency synthesizer and two alternating electric field output electrode electrical connections.
Priority Applications (1)
|Application Number||Priority Date||Filing Date||Title|
|CN201620736356.6U CN205786452U (en)||2016-07-09||2016-07-09||A kind of amount of viable cell measuring instrument|
Applications Claiming Priority (1)
|Application Number||Priority Date||Filing Date||Title|
|CN201620736356.6U CN205786452U (en)||2016-07-09||2016-07-09||A kind of amount of viable cell measuring instrument|
|Publication Number||Publication Date|
|CN205786452U true CN205786452U (en)||2016-12-07|
Family Applications (1)
|Application Number||Title||Priority Date||Filing Date|
|CN201620736356.6U Expired - Fee Related CN205786452U (en)||2016-07-09||2016-07-09||A kind of amount of viable cell measuring instrument|
Country Status (1)
|CN (1)||CN205786452U (en)|
- 2016-07-09 CN CN201620736356.6U patent/CN205786452U/en not_active Expired - Fee Related
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|C14||Grant of patent or utility model|
|CF01||Termination of patent right due to non-payment of annual fee|
|CF01||Termination of patent right due to non-payment of annual fee||
Granted publication date: 20161207
Termination date: 20200709