CN205786452U - A kind of amount of viable cell measuring instrument - Google Patents
A kind of amount of viable cell measuring instrument Download PDFInfo
- Publication number
- CN205786452U CN205786452U CN201620736356.6U CN201620736356U CN205786452U CN 205786452 U CN205786452 U CN 205786452U CN 201620736356 U CN201620736356 U CN 201620736356U CN 205786452 U CN205786452 U CN 205786452U
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- CN
- China
- Prior art keywords
- measuring instrument
- amount
- viable cell
- original position
- cell measuring
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The utility model discloses a kind of amount of viable cell measuring instrument, described measuring instrument includes popping one's head in (1) in situ, signals collecting amplifier (2), analog-digital converter (3) and processor (4), described original position probe (1) is for putting into measurement voltage in the liquid containing living cells, and (1) passes sequentially through signals collecting amplifier (2) to described original position probe, analog-digital converter (3) is connected with processor (4) signal.A kind of amount of viable cell measuring instrument of the present utility model can On-line sampling system living cells quantity.
Description
Technical field
This utility model relates to instrument analysis technology field, is specifically related to a kind of amount of viable cell measuring instrument.
Background technology
Reliably amount of viable cell is one of very important parameter in each sweat, that live, have metabolic activity
Cell quantity is closely related with production.All dynamic feeding strategy can be used to obtain higher cell in many sweats close
Degree and production concentration, these implementations of strategies all depend on the quantity of living cells, and therefore the measurement in real time of this key parameter is non-
The most important.Traditional amount of viable cell measuring method such as measures dry weight, absorbance and centrifugal volume etc., has operation complicated, delayed
Time length, measurement result are living cells and the shortcoming of dead cell summation.FDA discloses PAT industrial directory in JIUYUE, 2004, will
PAT is defined as one and can carry out design and analysis by measuring critical procedure parameter and quality index, control pharmaceutical production
The mechanism of journey and means.Online amount of viable cell measuring instrument application in terms of Biomass monitoring has become as in process analysis technique
Rising instrument.
Utility model content
The purpose of this utility model is to provide a kind of amount of viable cell measuring instrument, a kind of living cells of the present utility model to measure
Amount instrument can On-line sampling system living cells quantity.
For achieving the above object, this utility model 1, a kind of amount of viable cell measuring instrument, described measuring instrument includes popping one's head in situ,
Signals collecting amplifier, analog-digital converter and processor, described original position probe is measured for putting in the liquid containing living cells
Voltage, described original position probe passes sequentially through signals collecting amplifier, analog-digital converter is connected with processor signal.
Preferably, described original position probe includes two alternating electric field output electrodes and two voltage detecting electrodes.
Preferably, described measuring instrument also includes digital frequency synthesizer, described digital frequency synthesizer and two alternation electricity
Field output electrode electrical connection.
This utility model has the advantage that
The traditional measurement method of Biomass is all off-line, measures biomass concentration by off-line process cumbersome and time consuming, also
From fermentation tank, to extract sample, in sampling process, easily to bring miscellaneous bacteria into and infect fermentation tank, use another of off-line method
Individual shortcoming is, people can not realize automatically controlling sweat in this way.Online amount of viable cell measuring instrument is very
The problems referred to above of good solution.This instrument has wide spectrum, test point selects feature flexibly, and this is the most external commercial apparatus
Do not possess.In terms of the system integration, detection platform, data algorithm, combining closely of application are also main wounds
Newly.This instrument can provide powerful to vast laboratory and the amount of viable cell detecting instrument of motility (simultaneously also can be as biology
Other parameter detecting of suspension are used), meanwhile, this instrument miniaturization, production line easy for installation, with low cost, easy to use are former
Position measuring instrument also certainly will promote that promotion China biotechnology, in the popularization of the applications such as bioreactor, is produced by this technology
Industryization development is significant, and also has the advantage that
1. viable cell concentrations can be carried out that On-line sampling system, i.e. instrument record be biomass concentration at this very moment and
Not being the numerical value in a certain moment in the past, probe is inserted directly in fermented product without sampling in situ；
2. the stability worked long hours and reliability；
3. probe can be at working site high temperature sterilize in situ, and probe does not infects and pollutes fermented product in situ；
4. reading is affected little by graininess float and dead yeast cells；
5. (such as fermentation broth contents, pH value, dissolved oxygen concentration, temperature, mixing speed, ventilation when fermentation condition changes
Amounts etc. are when changing) reading of instrument is essentially unaffected.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of detailed description of the invention of amount of viable cell measuring instrument of the present utility model.
Detailed description of the invention
Detailed description below is used for illustrating this utility model, but is not limited to scope of the present utility model.
As it is shown in figure 1, this utility model provides a kind of amount of viable cell measuring instrument, described measuring instrument includes popping one's head in situ 1, letter
Number collection amplifier 2, analog-digital converter 3 and processor 4, described original position probe 1 is surveyed for putting in the liquid containing living cells
Amount voltage, described original position probe 1 passes sequentially through signals collecting amplifier 2, analog-digital converter 3 is connected with processor 4 signal.
Living cells has airtight cell membrane system, under the action of alternative electric field of certain frequency, in living cells charged carefully
Kytoplasm migrates, and runs into cell membrane complete, insulation and forms polarization phenomena, and cell becomes the most small capacitor, institute
The electric capacity surveyed becomes good linear relationship with the concentration of living cells.By measuring capacitance on electrode, demarcate reaction later and live thin
Born of the same parents' concentration.Described original position probe 1 can include two alternating electric field output electrodes and two voltage detecting electrodes.Described measuring instrument
Digital frequency synthesizer, described digital frequency synthesizer and two alternating electric field output electrode electrical connections can also be included.
Below by concrete amount of viable cell measuring instrument of the present utility model, but the most therefore limit this utility model.
Amount of viable cell measuring instrument, it is mainly by precision switch power circuit, digital frequency synthesizer, filter circuit, letter
Number output amplifier, signals collecting amplifying circuit, analog to digital conversion circuit, data caching circuit, central processing unit, communication electricity
Road, host computer and composition of popping one's head in situ.
Probe is made up of 4 electrodes in situ, and electrode is made up of platinum material.Digital frequency synthesizer output alternation
Electric field is carried on two electrodes in outside.In fermentation tank, living cells produces polarization phenomena under action of alternative electric field, in inner side
Detecting magnitude of voltage by signals collecting amplifying circuit on two electrodes, magnitude of voltage is converted into digital signal by analog-digital converter
After, after delivering to central processing unit process, obtain capacitance and electric conductivity value data, upload the data to by RS-485 communicating circuit
Host computer, host computer interface display curvilinear motion, judge viable cell concentrations more intuitively, visually, improve production efficiency.
This utility model uses precision switch power supply as power supply utility model, has work efficiency high, and it is excellent that volume is little etc.
Point.The Switching Power Supply selected must be high-quality, carries out the process of power supply ripple, otherwise directly has signals collecting the biggest
Impact.
This utility model communicating circuit uses RS-485 serial bus standard, and RS-485 interface uses balance driver and difference
Dividing the combination of receptor, noise immunity is good, and transfer rate is fast, long transmission distance, is well suited for using in industry spot.
Data analysis and process is mainly used all phase fast fourier transform algorithm, all phase Fu by this utility model
In leaf algorithm than conventional Fourier algorithm, there is more excellent suppression spectral leakage performance, strong interference immunity, precision is higher, be suitable for
In accurate measurement occasion, amount of calculation is little, is conducive to realizing real-time.
Digital frequency synthesizer uses ADI company AD9852 chip, and this chips has the programmable frequency of 48 and deposits
Device, 14 programmable phase offset registers, 12 programmable amplitude modulation and switch output shape keying function.The most permissible
Make instrument reach wide spectrum, frequency selects versatility performance flexibly.By PC control AD9852 output frequency and amplitude
Variable alternation cosine wave, simple, convenient.
Voltage on ADC sample circuit Real-time Collection probe medial electrode in situ, the central processing unit data to gathering are made
All phase fast Fourier transform, reference signal is also made all phase fast Fourier transform simultaneously, is calculated and survey by central processing unit
Measure signal and the phase contrast of reference signal and modulus value, calculate capacitance and electric conductivity value.
Communicating circuit uses ADI company magnetic coupling based on chip-scale transformator isolation technology, and communication delay is little, power consumption
Stability low, superpower, the life-span is long.RS-485 Interface design has lightning protection discharge tube, TVS protection, filter network tertiary treatment, can answer
To overwhelming majority thunderbolt, electromagnetic interference, electrostatic etc..
Although, the most with general explanation and specific embodiment, this utility model is made detailed description, but
On the basis of this utility model, can make some modifications or improvements it, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements on the basis of without departing from this utility model spirit, belong to this utility model and want
Seek the scope of protection.
Claims (3)
1. an amount of viable cell measuring instrument, it is characterised in that described measuring instrument includes popping one's head in situ (1), signals collecting amplifier
(2), analog-digital converter (3) and processor (4), described original position probe (1) measures electricity for putting in the liquid containing living cells
Pressure, (1) passes sequentially through signals collecting amplifier (2) to described original position probe, analog-digital converter (3) is connected with processor (4) signal.
Amount of viable cell measuring instrument the most according to claim 1, it is characterised in that described original position probe (1) includes two friendships
Changed electric field output electrode and two voltage detecting electrodes.
Amount of viable cell measuring instrument the most according to claim 1, it is characterised in that described measuring instrument also includes that numerical frequency is closed
Grow up to be a useful person, described digital frequency synthesizer and two alternating electric field output electrode electrical connections.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620736356.6U CN205786452U (en) | 2016-07-09 | 2016-07-09 | A kind of amount of viable cell measuring instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620736356.6U CN205786452U (en) | 2016-07-09 | 2016-07-09 | A kind of amount of viable cell measuring instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205786452U true CN205786452U (en) | 2016-12-07 |
Family
ID=58122296
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620736356.6U Expired - Fee Related CN205786452U (en) | 2016-07-09 | 2016-07-09 | A kind of amount of viable cell measuring instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205786452U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112666233A (en) * | 2020-12-09 | 2021-04-16 | 中南民族大学 | Algae detection system based on planar electrode array |
-
2016
- 2016-07-09 CN CN201620736356.6U patent/CN205786452U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112666233A (en) * | 2020-12-09 | 2021-04-16 | 中南民族大学 | Algae detection system based on planar electrode array |
| CN112666233B (en) * | 2020-12-09 | 2022-09-20 | 中南民族大学 | Algae detection system based on planar electrode array |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161207 Termination date: 20200709 |