CN206450690U - A kind of circulating tumor cell detector - Google Patents
A kind of circulating tumor cell detector Download PDFInfo
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- CN206450690U CN206450690U CN201720129634.6U CN201720129634U CN206450690U CN 206450690 U CN206450690 U CN 206450690U CN 201720129634 U CN201720129634 U CN 201720129634U CN 206450690 U CN206450690 U CN 206450690U
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- circulating tumor
- tumor cell
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Abstract
The utility model discloses a kind of circulating tumor cell detector, including instrument box, blood sample stocking mechanism, Beads enrichment mechanism, fluorescent staining mechanism and testing agency；Instrument box includes left box body and right case, and left box body and right case are respectively arranged with chamber door, and blood sample stocking mechanism, Beads enrichment mechanism, fluorescent staining mechanism are arranged in left box body；Blood sample stocking mechanism includes heparin tube, pipe clamp and supporting plate；Beads enrichment mechanism includes the magnetic bead kit and the magnetic field generator below magnetic bead kit for being used to separate circulating tumor cell；Fluorescent staining mechanism includes rotating shaft, fluorescent dye injection disk and fluorescent dye and holds bottle；Testing agency includes fluorescent electronic microscope and high-flux cell image analyzers.The utility model influences the factor of diagnostic result few, can effectively improve the precision of testing result by the circulating tumor cell in above-mentioned part detection detection blood.
Description
Technical field
The utility model is related to technical field of medical instruments, more particularly to a kind of circulating tumor cell detector.
Background technology
Circulating tumor cell (CTC) refers to depart from tumour and enters sanguimotor cancer cell, by detecting circulating tumor
The quantity of cell can assess cancer patient's disease development process and therapeutic effect.
The method of circulating tumor cell generally in detection blood has two kinds, and one of which method is using magnetic cell point
Selecting technology, its principle is first specific antibody to be coated on the magnetic bead of homologous secondary antibody immunity magnetic micropearls are made, then with target
Antigen binding on cell is into " target cell-antigen-antibody-magnetic bead " compound, and the compound has magnetic responsiveness, in additional magnetic
Move, target cell is enriched with, sorted using positive-selecting and the negative method for screening combination to certain orientation in the presence of
Go out target cell.Another method is to use immunocytochemical technique, and its principle is combined using antigen with antibody specificity
Feature, makes the antibody that coloring agent (fluorescein, enzyme etc.) is marked (mainly include epithelial cell angle egg with specific tumor markers
White CK, epithelial cell membrane specific antigen etc.) combine, tumour is judged by enzyme and substrate reactions colour developing or other coloration methods
The presence of cell, then tumour cell statistics is carried out by testing staff under the microscope.
Magnetic activated cell soring is simple to operate, and sub-elect without loss of functionality and vigor, can be immediately available for analysis and
Experiment, but the false positive rate analyzed is higher, detects not accurate enough.Immunocytochemical technique is ripe, but breaks up the swollen of difference
Knurl can not express cytokeratin and epithelial antigen in target antigen, rather than epithelial cell and may also be positive, detection
It is not accurate enough, and testing staff to result observe and interpretation it is subjective, count it is also not accurate enough.
Utility model content
The purpose of this utility model is to provide a kind of circulating tumor cell detector, can solved in above-mentioned background technology
The problem of existing,
The utility model is achieved in that a kind of circulating tumor cell detector, including the storage of instrument box, blood sample
Deposit mechanism, Beads enrichment mechanism, fluorescent staining mechanism and testing agency；
The instrument box includes left box body and right case, and the left box body and the right case are respectively arranged with chamber door,
The blood sample stocking mechanism, Beads enrichment mechanism, fluorescent staining mechanism are arranged in the left box body；
The blood sample stocking mechanism includes heparin tube, pipe clamp and supporting plate, and the supporting plate is horizontally set on described
In left box body, the upper surface of the supporting plate is provided with two adjusting levers, and two adjusting levers wear out the left box body
It is connected between top, and the top of described two adjusting levers on the outside of pull bar, the top of the left box body and is provided with two
Individual sleeve, two adjusting levers are arranged in the sleeve respectively, and adjustment bolt, the support are additionally provided with the sleeve
The one end of plate outside pointing to is evenly arranged with multiple pipe clamps, the pipe clamp and is provided with the heparin tube vertically, described to adopt
Blood vessel is clamped fixation by the pipe clamp, and the heparin tube is divided into two sections, and hypomere is conical pipe, and epimere is straight barrel type
Pipe, the bottom of the conical pipe is provided with liquid outlet, and the top of the straight barrel type pipe is provided with filling opening, the straight barrel type Guan Yu
Controlled valve is provided with above the filling opening；
The Beads enrichment mechanism is arranged at the lower section of the blood sample stocking mechanism, and the Beads enrichment mechanism includes
Magnetic bead kit and the magnetic field generator below the magnetic bead kit for separating circulating tumor cell, the left case
The bottom of body is provided with a raised platforms, and the magnetic field generator is arranged in the raised platforms, the magnetic bead kit
It is positioned on the raised platforms；
The fluorescent staining mechanism is arranged between the Beads enrichment mechanism and the blood sample stocking mechanism, described
Fluorescent staining mechanism includes rotating shaft, fluorescent dye injection disk and fluorescent dye and holds in bottle, the left box body and set
Rotating shaft is stated, the rotating shaft is vertically provided in the left box body, fixed is covered in the rotating shaft has the fluorescent dye to inject surely
Disk, the fluorescent dye holds the side that bottle is positioned over the raised platforms, is provided with fluorescent dye injection disk multiple
The inlet of indent, the lower end of the inlet is provided with downward tapered mouth, and the bottom of the tapered mouth is provided with liquid outlet；
The testing agency is arranged in right case, and the testing agency includes fluorescent electronic microscope and high-flux cell
Image analyzers, the fluorescent electronic microscope is electrically connected with the high-flux cell image analyzers.
Further, the pipe clamp is the clamp being made up of two jig arm being hinged.
Further, the fluorescent dye holds and fluorescent dye is provided with bottle, and the fluorescent dye is used to mark
Circulating tumor cell.
Further, it is enclosed with the antibody immune magnetic beads being placed with the magnetic bead kit on immunomagnetic beads, immunomagnetic beads
Antibody for separating circulating tumor cell.
The utility model has the advantages that：
The utility model provides a kind of circulating tumor cell detector, is divided circulating tumor cell by immunomagnetic beads method
From by fluorescent dye mark cycle tumour cell, the circulating tumor cell after mark is obtained using fluorescent electronic microscope
The distributed image of circulating tumor cell is taken, image is passed into high-flux cell image analyzers, high-flux cell image is utilized
Analyzer analyzes the quantity for drawing circulating tumor cell, and reliability is high.The circulating tumor cell inspection provided due to the utility model
Instrument is surveyed, by the circulating tumor cell in above-mentioned part detection detection blood, influences the factor of diagnostic result few, so, no
Easily there is the case history of false positive, so can effectively improve the precision of testing result.In addition, the circulating tumor cell is detected
Instrument is by setting multiple heparin tubes gripped using pipe clamp, and heparin tube can be taken a blood sample by the mobile realization of adjusting lever
The height adjustment of pipe, can facilitate tester to be operated, the dismounting and cleaning of heparin tube be also allowed for, while being also convenient for multiple
The detection of blood sample.
Brief description of the drawings
A kind of circulating tumor cell detector structural representation that Fig. 1 provides for the utility model.
Description of reference numerals：
1st, left box body；2nd, right case；3rd, chamber door；4th, testing agency；5th, heparin tube；6th, pipe clamp；7th, supporting plate；8th, adjust
Bar；9th, pull bar；10th, sleeve；11st, adjustment bolt；12nd, controlled valve；13rd, magnetic bead kit；14th, magnetic field generator；15th, it is raised
Platform；16th, rotating shaft；17th, fluorescent dye injection disk；18th, fluorescent dye holds bottle.
Embodiment
Below in conjunction with the accompanying drawings, an embodiment of the present utility model is described in detail, it is to be understood that this
The protection domain of utility model is not limited by embodiment.
A kind of circulating tumor cell detector structural representation that Fig. 1 provides for the utility model embodiment.The circulation is swollen
Oncocyte detector is specifically included：Instrument box, blood sample stocking mechanism, Beads enrichment mechanism, fluorescent staining mechanism and inspection
Survey mechanism 4.
As shown in figure 1, instrument box includes left box body 1 and right case 2, left box body 1 and right case 2 are respectively arranged with chamber door
3, blood sample stocking mechanism, Beads enrichment mechanism, fluorescent staining mechanism are arranged in left box body 1；
Blood sample stocking mechanism includes heparin tube 5, pipe clamp 6 and supporting plate 7, and supporting plate 7 is horizontally set on left box body 1
Interior, the upper surface of supporting plate 7 is provided with two adjusting levers 8, and two adjusting levers 8 wear out the top of left box body 1, and two tune
It is connected between the top of whole bar 8 on the outside of pull bar 9, the top of left box body 1 and is provided with two sleeves 10, two adjusting levers 8 is distinguished
It is arranged in sleeve 10, adjustment bolt 11 is additionally provided with sleeve 10, the one end of supporting plate 7 outside pointing to is evenly arranged with many
Heparin tube 5 is vertically arranged with individual pipe clamp 6, pipe clamp 6, heparin tube 5 is clamped fixation by pipe clamp 6, above and below 5 points of heparin tube is
Two sections, hypomere is conical pipe, and epimere is straight barrel type pipe, and the bottom of conical pipe is provided with liquid outlet, and the top of straight barrel type pipe is set
There is filling opening, straight barrel type pipe is provided with controlled valve 12 above filling opening；Structure design as heparin tube 5, on the one hand
It is easy to the collection of blood sample, is on the other hand easy to control the discharge of blood sample to control using this heparin tube 5.
Beads enrichment mechanism is arranged at the lower section of blood sample stocking mechanism, and Beads enrichment mechanism includes being used to separate circulation
The magnetic bead kit 13 of tumour cell and the magnetic field generator 14 in the lower section of magnetic bead kit 13, magnetic field generator 14 are set
Put, can make to produce magnetic field in left box body 1, the bottom of left box body 1 is provided with a raised platforms 15, and magnetic field generator 14 is set
In in raised platforms 15, magnetic bead kit 13 is positioned on raised platforms 15；
Fluorescent staining mechanism is arranged between Beads enrichment mechanism and blood sample stocking mechanism, and fluorescent staining mechanism includes
Rotating shaft 16, fluorescent dye injection disk 17 and fluorescent dye are held and rotating shaft 16 are provided with bottle 18, left box body 1, and rotating shaft 16 is erected
To being arranged in left box body 1, set is even fixed with fluorescent dye injection disk 17 in rotating shaft 16, and fluorescent dye holds bottle 18 and placed
In the inlet that multiple indents are provided with the side of raised platforms 15, fluorescent dye injection disk 17, the lower end of inlet is set
Downward tapered mouth is equipped with, the bottom of tapered mouth is provided with liquid outlet；Fluorescent dye injects the setting of disk 17, can be conveniently accurate
The addition of true progress fluorescent dye.
Testing agency 4 is arranged in right case 2, and testing agency 4 includes fluorescent electronic microscope and high-flux cell image
Analyzer, fluorescent electronic microscope is electrically connected with high-flux cell image analyzers.
Pipe clamp 6 is the clamp being made up of two jig arm being hinged.
Fluorescent dye holds and fluorescent dye is provided with bottle 18, and fluorescent dye is used for mark cycle tumour cell.
It is enclosed with and is followed for separation on the antibody immune magnetic beads being placed with magnetic bead kit 13 on immunomagnetic beads, immunomagnetic beads
The antibody of ring tumour cell.
Magnetic bead kit 13, high-flux cell image analyzers, fluorescent electronic microscope are prior art, are not done herein
It is described in detail.
The operation principle of the circulating tumor cell detector is：First by for collecting, the multiple of storage of blood sample adopt
Blood vessel 5 is gripped by pipe clamp 6, then by being lifted to pull bar 9, is adjusted to proper height position, is passed through
Adjustment bolt 11 is fixed to carry out position to adjusting lever 8, is then inserted in heparin tube 5 blood sample to be detected, is now adopted
The controlled valve 12 of blood vessel 5 is closed, and can place cell-protecting in heparin tube 5 in advance, utilizes the storage of blood sample
Deposit.After multiple blood samples are collected by heparin tube 5, fluorescent dye injection disk 17 is gone in the presence of rotating shaft 16
On one side, then magnetic bead kit 13 is positioned over to the lower section of heparin tube 5, the height of heparin tube 5 is then adjusted again by pull bar 9
Position is spent, the lower end and magnetic bead kit 13 for making heparin tube 5 are close to correct position, now, need to such as detected in which heparin tube 5
Blood sample, it is possible to open the controlled valve 12 of corresponding heparin tube 5, the blood sample in heparin tube 5 is entered magnetic bead
In kit 13, immunomagnetic beads is compound into " target cell-antigen-antibody-magnetic bead " with the antigen binding on target cell in sample blood
Thing, starts magnetic field generator 14, and then the compound is enriched with magnetic fields, is separated；Then blood sampling is adjusted again
After the height and position of pipe 5, fluorescent dye injection disk 17 is gone in the top of magnetic bead kit 13, disk is injected by fluorescent dye
Fluorescent dye is injected into magnetic bead kit 13 by the inlet on 17, fluorescent dye mark cycle tumour cell, is entered
One step sub-elects circulating tumor cell, and the circulating tumor cell after mark then is placed on into the microscopical detection of fluorescent electronic
Place, so as to obtain the distributed image of circulating tumor cell using fluorescent electronic microscope, then passes to high flux thin by image
Born of the same parents' image analyzers, the quantity for drawing circulating tumor cell is analyzed using high-flux cell image analyzers.
In summary, a kind of circulating tumor cell detector that the utility model embodiment is provided, passes through immunomagnetic beads method
Circulating tumor cell is separated, by fluorescent dye mark cycle tumour cell, the circulating tumor cell after mark utilized
Fluorescent electronic microscope obtains the distributed image of circulating tumor cell, and image is passed into high-flux cell image analyzers, profit
The quantity for drawing circulating tumor cell is analyzed with high-flux cell image analyzers, reliability is high.Because the utility model is provided
Circulating tumor cell detector, pass through the circulating tumor cell in above-mentioned part detection detection blood, influence diagnostic result
Factor it is few, so, the case history of false positive is less prone to, so the precision of testing result can be improved effectively.In addition, should
Circulating tumor cell detector is by setting multiple heparin tubes gripped using pipe clamp 6, and heparin tube can be by adjusting
The movement of whole bar realize heparin tube height adjustment, tester can be facilitated to be operated, also allow for heparin tube dismounting and clearly
Wash, while being also convenient for the detection to multiple blood samples.
Disclosed above is only several specific embodiments of the present utility model, still, the utility model embodiment not office
It is limited to this, the changes that any person skilled in the art can think of should all fall into protection domain of the present utility model.
Claims (4)
1. a kind of circulating tumor cell detector, it is characterised in that including instrument box, blood sample stocking mechanism, magnetic bead point
Disembark structure, fluorescent staining mechanism and testing agency；
The instrument box includes left box body and right case, and the left box body and the right case are respectively arranged with chamber door, described
Blood sample stocking mechanism, Beads enrichment mechanism, fluorescent staining mechanism are arranged in the left box body；
The blood sample stocking mechanism includes heparin tube, pipe clamp and supporting plate, and the supporting plate is horizontally set on the left case
In vivo, the upper surface of the supporting plate is provided with two adjusting levers, and two adjusting levers wear out the top of the left box body,
And it is connected between the top of two adjusting levers on the outside of pull bar, the top of the left box body and is provided with two sleeves, two
The individual adjusting lever is arranged in the sleeve respectively, adjustment bolt is additionally provided with the sleeve, the supporting plate is in sensing
Outside one end is evenly arranged with multiple pipe clamps, the pipe clamp is provided with the heparin tube vertically, and the heparin tube passes through
The pipe clamp is clamped fixation, and the heparin tube is divided into two sections, and hypomere is conical pipe, and epimere is straight barrel type pipe, described
The bottom of conical pipe is provided with liquid outlet, and the top of the straight barrel type pipe is provided with filling opening, described in the straight barrel type Guan Yu plus
The top of liquid mouthful is provided with controlled valve；
The Beads enrichment mechanism is arranged at the lower section of the blood sample stocking mechanism, and the Beads enrichment mechanism includes being used for
The magnetic bead kit and the magnetic field generator below the magnetic bead kit of circulating tumor cell are separated, the left box body
Bottom is provided with a raised platforms, and the magnetic field generator is arranged in the raised platforms, and the magnetic bead kit is placed
On the raised platforms；
The fluorescent staining mechanism is arranged between the Beads enrichment mechanism and the blood sample stocking mechanism, the fluorescence
Dyeing mechanism holds including rotating shaft, fluorescent dye injection disk and fluorescent dye and described turn is provided with bottle, the left box body
Axle, the rotating shaft is vertically provided in the left box body, and set is connected with the fluorescent dye injection disk in the rotating shaft, described glimmering
Light coloring agent holds and is provided with multiple indents on the side that bottle is positioned over the raised platforms, the fluorescent dye injection disk
Inlet, the lower end of the inlet is provided with downward tapered mouth, and the bottom of the tapered mouth is provided with liquid outlet；
The testing agency is arranged in right case, and the testing agency includes fluorescent electronic microscope and high-flux cell image
Analyzer, the fluorescent electronic microscope is electrically connected with the high-flux cell image analyzers.
2. a kind of circulating tumor cell detector as claimed in claim 1, it is characterised in that the pipe clamp is mutual by two
The clamp that the jig arm being hinged is constituted.
3. a kind of circulating tumor cell detector as claimed in claim 1, it is characterised in that the fluorescent dye holds bottle
Fluorescent dye is inside provided with, the fluorescent dye is used for mark cycle tumour cell.
4. a kind of circulating tumor cell detector as claimed in claim 1, it is characterised in that be placed with the magnetic bead kit
The antibody for separating circulating tumor cell is enclosed with antibody immune magnetic beads on immunomagnetic beads, immunomagnetic beads.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720129634.6U CN206450690U (en) | 2017-02-09 | 2017-02-09 | A kind of circulating tumor cell detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720129634.6U CN206450690U (en) | 2017-02-09 | 2017-02-09 | A kind of circulating tumor cell detector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206450690U true CN206450690U (en) | 2017-08-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720129634.6U Expired - Fee Related CN206450690U (en) | 2017-02-09 | 2017-02-09 | A kind of circulating tumor cell detector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206450690U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111896512A (en) * | 2020-08-10 | 2020-11-06 | 中南大学湘雅医院 | Lung cancer circulating tumor cell detection equipment |
| CN113040769A (en) * | 2021-03-23 | 2021-06-29 | 王凯 | Liquid biopsy detection system and method for circulating tumor cells based on 5G and block chain |
| CN116358980A (en) * | 2023-06-01 | 2023-06-30 | 翔鹏佑康(北京)科技有限公司 | Tumor cell short-term test identification device |
-
2017
- 2017-02-09 CN CN201720129634.6U patent/CN206450690U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111896512A (en) * | 2020-08-10 | 2020-11-06 | 中南大学湘雅医院 | Lung cancer circulating tumor cell detection equipment |
| CN113040769A (en) * | 2021-03-23 | 2021-06-29 | 王凯 | Liquid biopsy detection system and method for circulating tumor cells based on 5G and block chain |
| CN116358980A (en) * | 2023-06-01 | 2023-06-30 | 翔鹏佑康(北京)科技有限公司 | Tumor cell short-term test identification device |
| CN116358980B (en) * | 2023-06-01 | 2023-09-29 | 翔鹏佑康(北京)科技有限公司 | Tumor cell short-term test identification device |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170829 Termination date: 20180209 |
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| CF01 | Termination of patent right due to non-payment of annual fee |