CN114052179A - Quinoa and litsea cubeba meal replacement porridge and preparation method thereof - Google Patents
Quinoa and litsea cubeba meal replacement porridge and preparation method thereof Download PDFInfo
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- CN114052179A CN114052179A CN202111227891.0A CN202111227891A CN114052179A CN 114052179 A CN114052179 A CN 114052179A CN 202111227891 A CN202111227891 A CN 202111227891A CN 114052179 A CN114052179 A CN 114052179A
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- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
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Abstract
The invention discloses quinoa-litsea cubeba-rattan meal replacement porridge which comprises the following raw materials in parts by weight: 60-80 parts of quinoa, 25-45 parts of corn starch, 200 parts of litsea cubeba 150-. The quinoa-litsea cubeba meal replacement porridge prepared by the invention has the characteristics of special flavor, such as high calcium, high protein and the like, can be quickly brewed and eaten by adding a proper amount of water, has an excellent effect of reducing uric acid in a short-term use, and can be widely popularized and applied.
Description
Technical Field
The invention belongs to the technical field of preparation of meal replacement porridge, and particularly relates to quinoa and litsea cubeba meal replacement porridge and a preparation method thereof.
Background
The meal replacement (also called meal replacement and meal replacement food) is the food for replacing part or all dinners as the name suggests, and common meal replacement forms comprise meal replacement powder, meal replacement bars, meal replacement milkshakes, meal replacement porridge and the like. The meal replacement can quickly and conveniently provide a large amount of various nutrient substances for the human body, and has the characteristics of high fiber, low calorie, easy satiety and the like. Such as fresh flower pie produced by using konjak as raw material, and coarse cereal powder obtained by grinding coarse cereals.
Hyperuricemia is caused by increased uric acid production and/or decreased renal uric acid secretion, which is the most dangerous factor for inducing gout. The use of drugs to reduce blood uric acid concentrations is one of the common methods for preventing gout development. At present, the drugs for treating hyperuricemia mainly comprise colchicine, non-sterol anti-inflammatory drugs, uric acid production inhibiting drugs, uric acid excretion promoting drugs, glucocorticoid and the like. However, these drugs have certain defects in treatment, poor curative effect and great side effect, which become the bottleneck of clinical application, and have difficulty in reducing uric acid or preventing uric acid increase for a long time.
At present, the functional meal replacement porridge for reducing uric acid is produced in an industrialized mode by taking the litsea cubeba as a main raw material, and the problem that the quality guarantee period is short and the like exists, so that the functional meal replacement porridge is not beneficial to popularization and application.
Disclosure of Invention
The invention aims to provide quinoa and litsea cubeba meal replacement porridge and a preparation method thereof, and aims to solve the problems that the meal replacement porridge produced by the prior art is short in quality guarantee period and the like.
In order to solve the technical problems, the invention adopts the following technical scheme:
the quinoa-litsea cubeba meal replacement porridge comprises the following raw materials in parts by weight: 60-80 parts of quinoa, 25-45 parts of corn starch, 200 parts of litsea cubeba 150-.
Preferably, the quinoa-litsea cubeba meal replacement porridge comprises the following raw materials in parts by weight: 75 parts of quinoa, 40 parts of corn starch, 160 parts of litsea cubeba, 27 parts of dendrobium moniliforme, 12 parts of gynura divaricata, 18 parts of angelica sinensis leaves, 450 parts of water, 1.6 parts of vitamin C, 5 parts of 4-O-alpha-D-glucopyranosyl-D-glucose, 0.4 part of polyglycerol polyricinoleate and 1 part of sodium alginate.
The invention also provides a preparation method of the quinoa-litsea cubeba meal replacement porridge, which comprises the following steps:
s1: weighing the Siberian cocklebur stems, the dendrobium candidum, the gynura bicolor and the angelica leaves according to the weight part ratio, and then mixing the raw materials to obtain the mixed raw materials;
s2: placing the mixed raw materials obtained in the step S1 into water at 86-90 ℃ for blanching for 1-2min, taking out, placing into a color protection solution for color protection, and obtaining a color protection raw material;
s3: pulping the color protection raw material prepared in the step S2 to prepare a pulping material;
s4: adding complex enzyme into the pulped material prepared in the step S3, and performing enzymolysis for 1-1.5h at the temperature of 48-55 ℃ to prepare an enzymolysis material;
s5: grinding the enzymolysis material prepared in the step S4 in a colloid mill to prepare a ground material;
s6: adding auxiliary materials into the ground material prepared in the step S5, wherein the auxiliary materials comprise 500 parts of vitamin C, 4-O-alpha-D-glucopyranosyl-D-glucose, polyglycerol polyricinoleate, sodium alginate and water, and then blending for 8-10min at the rotation speed of 200-300r/min to prepare blending liquid;
s7: homogenizing the prepared solution obtained in the step S6 by using a homogenizer, and filtering to obtain a homogenized solution;
s8: and (4) carrying out vacuum freeze drying on the homogenized liquid prepared in the step (S7) under the conditions of: the temperature of the cold trap is between 28 ℃ below zero and 32 ℃ below zero, the heating temperature is between 31 ℃ and 34 ℃, the vacuum degree is 140Pa, and the materials are dried until the moisture content of the materials is less than or equal to 1.2 percent to prepare dry powder;
s9: quinoa pretreatment: weighing quinoa according to the weight part ratio, enriching gamma-aminobutyric acid (GABA) after the quinoa germinates, and fermenting the germinated quinoa by utilizing lactic acid bacteria to further enrich GABA to prepare pretreated quinoa;
s10: adding the dry powder prepared in the step S8 and corn starch into the preprocessed quinoa prepared in the step S9 for compounding to prepare a compound material;
s11: and (5) drying, sterilizing and packaging the compound material prepared in the step (S10) to prepare the quinoa-litsea cubeba meal replacement porridge capable of being brewed quickly.
Preferably, the mass ratio of the mixed raw materials to the color protection liquid in the step S2 is 1: 2-3.
Preferably, the color protection solution is an aqueous solution containing 0.1-0.2% by mass of sodium erythorbate, 2.2-3% by mass of leucine, 1.3-1.8% by mass of amino-oligosaccharin and 0.2-0.4% by mass of sodium phytate.
Preferably, the amount of the complex enzyme added into the pulping material in the step S4 is 0.3-0.5% of the total weight of the pulping material.
Preferably, the complex enzyme consists of pectinase, alpha-amylase, papain and transglucosidase in a mass ratio of 6-9:3-5:1-2: 2-3.
Preferably, in step S5, the enzymatic hydrolysate is ground in a colloid mill to a fineness of less than 20 μm to obtain a ground material.
Preferably, the blending solution is homogenized by a high-pressure homogenizer at 20-35MP in step S7 to obtain a homogenized solution.
Preferably, the lactic acid bacteria account for 3% -6% of the weight of the germinated quinoa in step S9.
The invention has the following beneficial effects:
(1) 4-O-alpha-D-glucopyranosyl-D-glucose, polyglycerol polyricinoleate and sodium alginate in the homogeneous solution prepared by the invention generate a synergistic effect when used together, and the centrifugal precipitation rate is synergistically reduced because: 4-O-alpha-D-glucopyranosyl-D-glucose can have high affinity with protein particles, and a sugar film can be formed on the surface of protein after the 4-O-alpha-D-glucopyranosyl-D-glucose is added, so that the affinity of the protein and a dispersion medium is improved, the protein is stably and uniformly dispersed in the homogeneous solution, and the centrifugal precipitation rate is further reduced; polyglycerol polyricinoleate molecules are directionally adsorbed towards the surface of water oil to form a stable complex, the adsorption capacity is increased, the surface tension is reduced, the aggregation among particles in homogeneous liquid is prevented, the formation of a sugar film on the surfaces of 4-O-alpha-D-glucopyranosyl-D-glucose and protein is promoted to be enhanced, and the centrifugal precipitation rate is reduced; the sodium alginate can inhibit protein particles in the homogeneous liquid from interacting to form colloid, so that the protein particles are in a negative charge state as a whole, and the homogeneous liquid provided by the invention is kept in a stable state under the repulsion action. Therefore, under the interaction of 4-O-alpha-D-glucopyranosyl-D-glucose, polyglycerol ricinoleate and sodium alginate, the centrifugal precipitation rate of the homogenized solution is synergistically reduced, the stability of the meal replacement porridge prepared by the homogenized solution is improved, and the quality guarantee period is prolonged.
(2) After the raw quinoa is pretreated (germinated and fermented after germination), the GABA content is increased to over 199.41mg/100g, which shows that the nutritional ingredients of the raw quinoa are greatly increased after the raw quinoa is pretreated, and the additional value of the meal replacement porridge can be improved.
(3) The invention takes quinoa and litsea cubeba as main raw materials, combines the special fruits and vegetables such as dendrobium candidum, gynura bicolor and angelica sinensis leaves to prepare meal replacement porridge, has the characteristics of special flavor, such as high calcium, high protein and the like, can be quickly brewed and eaten by adding proper amount of water, has excellent effect of reducing uric acid in short-term use, and can be widely popularized and applied.
Detailed Description
For a better understanding of the present invention, the following examples are given to illustrate, but not to limit the scope of the present invention.
In the following embodiments, the quinoa-litsea cubeba meal replacement porridge comprises the following raw materials in parts by weight: 60-80 parts of quinoa, 25-45 parts of corn starch, 200 parts of litsea cubeba 150-;
the preparation method of the quinoa-litsea cubeba meal replacement porridge comprises the following steps:
s1: weighing the Siberian cocklebur stems, the dendrobium candidum, the gynura bicolor and the angelica leaves according to the weight part ratio, and then mixing the raw materials to obtain the mixed raw materials;
s2: placing the mixed raw materials obtained in the step S1 into water at 86-90 ℃ for blanching for 1-2min, taking out the raw materials, placing the raw materials into a color protection solution for color protection, and obtaining a color protection raw material, wherein the mass ratio of the mixed raw materials to the color protection solution is 1:2-3, and the color protection solution is an aqueous solution containing 0.1-0.2% by mass of sodium erythorbate, 2.2-3% by mass of leucine, 1.3-1.8% by mass of amino-oligosaccharide and 0.2-0.4% by mass of sodium phytate;
s3: pulping the color protection raw material prepared in the step S2 to prepare a pulping material;
s4: adding a complex enzyme which is 0.3-0.5% of the total weight of the pulping material into the pulping material prepared in the step S3, and carrying out enzymolysis for 1-1.5h at the temperature of 48-55 ℃ to prepare an enzymolysis material, wherein the complex enzyme consists of pectinase, alpha-amylase, papain and glucose transglycosidase in a mass ratio of 6-9:3-5:1-2: 2-3;
s5: grinding the enzymolysis material prepared in the step S4 in a colloid mill to the fineness of less than 20 mu m to prepare a ground material;
s6: adding auxiliary materials into the ground material prepared in the step S5, wherein the auxiliary materials comprise 500 parts of vitamin C, 4-O-alpha-D-glucopyranosyl-D-glucose, polyglycerol polyricinoleate, sodium alginate and water, and then blending for 8-10min at the rotation speed of 200-300r/min to prepare blending liquid;
s7: homogenizing the prepared solution obtained in step S6 with a high pressure homogenizer at 20-35MP, and filtering to obtain homogenized solution;
s8: and (4) carrying out vacuum freeze drying on the homogenized liquid prepared in the step (S7) under the conditions of: the temperature of the cold trap is between 28 ℃ below zero and 32 ℃ below zero, the heating temperature is between 31 ℃ and 34 ℃, the vacuum degree is 140Pa, and the materials are dried until the moisture content of the materials is less than or equal to 1.2 percent to prepare dry powder;
s9: quinoa pretreatment: weighing quinoa according to a weight part ratio, enriching gamma-aminobutyric acid (GABA) after the quinoa germinates, and then fermenting the germinated quinoa by utilizing lactic acid bacteria to further enrich GABA to prepare pretreated quinoa, wherein the lactic acid bacteria account for 3% -6% of the weight of the germinated quinoa;
s10: adding the dry powder prepared in the step S8 and corn starch into the preprocessed quinoa prepared in the step S9 for compounding to prepare a compound material;
s11: and (5) drying, sterilizing and packaging the compound material prepared in the step (S10) to prepare the quinoa-litsea cubeba meal replacement porridge capable of being brewed quickly.
The following is a more specific example.
Example 1
The quinoa-litsea cubeba meal replacement porridge comprises the following raw materials in parts by weight: 78 parts of quinoa, 43 parts of corn starch, 190 parts of litsea cubeba, 28 parts of dendrobium candidum, 15 parts of gynura divaricata, 24 parts of angelica sinensis leaves, 480 parts of water, 2 parts of vitamin C, 5 parts of 4-O-alpha-D-glucopyranosyl-D-glucose, 0.5 part of polyglycerol ricinoleate and 1.2 parts of sodium alginate;
the preparation method of the quinoa-litsea cubeba meal replacement porridge comprises the following steps:
s1: weighing the Siberian cocklebur stems, the dendrobium candidum, the gynura bicolor and the angelica leaves according to the weight part ratio, and then mixing the raw materials to obtain the mixed raw materials;
s2: placing the mixed raw materials obtained in the step S1 into water at the temperature of 90 ℃ for blanching for 1min, taking out the raw materials, placing the raw materials into a color protection solution for color protection, and obtaining a color protection raw material, wherein the mass ratio of the mixed raw materials to the color protection solution is 1:2, and the color protection solution contains 0.2 mass percent of sodium erythorbate, 2.9 mass percent of leucine, 1.7 mass percent of amino-oligosaccharide and 0.4 mass percent of sodium phytate aqueous solution;
s3: pulping the color protection raw material prepared in the step S2 to prepare a pulping material;
s4: adding a complex enzyme which is 0.5 percent of the total weight of the pulping material into the pulping material prepared in the step S3, and carrying out enzymolysis for 1h at the temperature of 54 ℃ to prepare an enzymolysis material, wherein the complex enzyme consists of pectinase, alpha-amylase, papain and transglucosidase in a mass ratio of 9:4:1: 3;
s5: grinding the enzymolysis material prepared in the step S4 in a colloid mill to the fineness of below 19.7 mu m to prepare a ground material;
s6: adding auxiliary materials into the ground material prepared in the step S5, wherein the auxiliary materials comprise vitamin C, 4-O-alpha-D-glucopyranosyl-D-glucose, polyglycerol polyricinoleate, sodium alginate and 480 parts of water, and then preparing for 9min at the rotating speed of 300r/min to prepare a prepared liquid;
s7: homogenizing the prepared solution obtained in step S6 with a high-pressure homogenizer at 35MP, and filtering to obtain a homogenized solution;
s8: and (4) carrying out vacuum freeze drying on the homogenized liquid prepared in the step (S7) under the conditions of: the cold trap temperature is minus 28 ℃, the heating temperature is 31 ℃, the vacuum degree is 120Pa, and the materials are dried until the moisture content of the materials is 1 percent to prepare dry powder;
s9: quinoa pretreatment: weighing quinoa according to a weight part ratio, enriching gamma-aminobutyric acid (GABA) after the quinoa germinates, and then fermenting the germinated quinoa by utilizing lactic acid bacteria to further enrich GABA to prepare pretreated quinoa, wherein the lactic acid bacteria account for 6% of the weight of the germinated quinoa;
s10: adding the dry powder prepared in the step S8 and corn starch into the preprocessed quinoa prepared in the step S9 for compounding to prepare a compound material;
s11: and (5) drying, sterilizing and packaging the compound material prepared in the step (S10) to prepare the quinoa-litsea cubeba meal replacement porridge capable of being brewed quickly.
Example 2
The quinoa-litsea cubeba meal replacement porridge comprises the following raw materials in parts by weight: 72 parts of quinoa, 36 parts of corn starch, 180 parts of litsea cubeba, 26 parts of dendrobium candidum, 13 parts of gynura divaricata, 20 parts of angelica sinensis leaves, 420 parts of water, 1.2 parts of vitamin C, 4 parts of 4-O-alpha-D-glucopyranosyl-D-glucose, 0.3 part of polyglycerol ricinoleate and 0.8 part of sodium alginate;
the preparation method of the quinoa-litsea cubeba meal replacement porridge comprises the following steps:
s1: weighing the Siberian cocklebur stems, the dendrobium candidum, the gynura bicolor and the angelica leaves according to the weight part ratio, and then mixing the raw materials to obtain the mixed raw materials;
s2: putting the mixed raw materials obtained in the step S1 into water with the temperature of 87 ℃ for blanching for 2min, then fishing out and putting into color protection liquid for color protection to obtain a color protection raw material, wherein the mass ratio of the mixed raw materials to the color protection liquid is 1:2, and the color protection liquid is an aqueous solution containing 0.1 mass percent of sodium erythorbate, 2.4 mass percent of leucine, 1.6 mass percent of amino-oligosaccharide and 0.2 mass percent of sodium phytate;
s3: pulping the color protection raw material prepared in the step S2 to prepare a pulping material;
s4: adding a complex enzyme which is 0.3 percent of the total weight of the pulping material into the pulping material prepared in the step S3, and carrying out enzymolysis for 1.4 hours at the temperature of 50 ℃ to prepare an enzymolysis material, wherein the complex enzyme consists of pectinase, alpha-amylase, papain and transglucosidase in a mass ratio of 7:3:1: 2;
s5: grinding the enzymolysis material prepared in the step S4 in a colloid mill to the fineness of below 19.2 mu m to prepare a ground material;
s6: adding auxiliary materials into the ground material prepared in the step S5, wherein the auxiliary materials comprise vitamin C, 4-O-alpha-D-glucopyranosyl-D-glucose, polyglycerol polyricinoleate, sodium alginate and 420 parts of water, and then preparing for 10min at the rotating speed of 200r/min to prepare a prepared liquid;
s7: homogenizing the prepared solution obtained in step S6 with a high-pressure homogenizer at 22MP, and filtering to obtain a homogenized solution;
s8: and (4) carrying out vacuum freeze drying on the homogenized liquid prepared in the step (S7) under the conditions of: the cold trap temperature is-30 ℃, the heating temperature is 32 ℃, the vacuum degree is 130Pa, and the materials are dried until the moisture content of the materials is 0.9 percent to prepare dry powder;
s9: quinoa pretreatment: weighing quinoa according to a weight part ratio, enriching gamma-aminobutyric acid (GABA) after the quinoa germinates, and then fermenting the germinated quinoa by utilizing lactic acid bacteria to further enrich GABA to prepare pretreated quinoa, wherein the lactic acid bacteria account for 4% of the weight of the germinated quinoa;
s10: adding the dry powder prepared in the step S8 and corn starch into the preprocessed quinoa prepared in the step S9 for compounding to prepare a compound material;
s11: and (5) drying, sterilizing and packaging the compound material prepared in the step (S10) to prepare the quinoa-litsea cubeba meal replacement porridge capable of being brewed quickly.
Example 3
The quinoa-litsea cubeba meal replacement porridge comprises the following raw materials in parts by weight: 75 parts of quinoa, 40 parts of corn starch, 160 parts of litsea cubeba, 27 parts of dendrobium candidum, 12 parts of gynura divaricata, 18 parts of angelica sinensis leaves, 450 parts of water, 1.6 parts of vitamin C, 5 parts of 4-O-alpha-D-glucopyranosyl-D-glucose, 0.4 part of polyglycerol ricinoleate and 1 part of sodium alginate;
the preparation method of the quinoa-litsea cubeba meal replacement porridge comprises the following steps:
s1: weighing the Siberian cocklebur stems, the dendrobium candidum, the gynura bicolor and the angelica leaves according to the weight part ratio, and then mixing the raw materials to obtain the mixed raw materials;
s2: placing the mixed raw materials obtained in the step S1 into water at the temperature of 88 ℃ for blanching for 1.4min, then fishing out and placing into a color protection solution for color protection to obtain a color protection raw material, wherein the mass ratio of the mixed raw materials to the color protection solution is 1:2.6, and the color protection solution contains 0.2 mass percent of sodium erythorbate, 2.8 mass percent of leucine, 1.6 mass percent of amino-oligosaccharide and 0.3 mass percent of sodium phytate aqueous solution;
s3: pulping the color protection raw material prepared in the step S2 to prepare a pulping material;
s4: adding a complex enzyme which is 0.4 percent of the total weight of the pulping material into the pulping material prepared in the step S3, and carrying out enzymolysis for 1.3h at the temperature of 52 ℃ to prepare an enzymolysis material, wherein the complex enzyme consists of pectinase, alpha-amylase, papain and transglucosidase in a mass ratio of 8:4:2: 3;
s5: grinding the enzymolysis material prepared in the step S4 in a colloid mill to the fineness of below 18.6 mu m to prepare a ground material;
s6: adding auxiliary materials into the ground material prepared in the step S5, wherein the auxiliary materials comprise vitamin C, 4-O-alpha-D-glucopyranosyl-D-glucose, polyglycerol polyricinoleate, sodium alginate and 450 parts of water, and then blending for 8min at the rotating speed of 300r/min to prepare blended liquid;
s7: homogenizing the prepared solution obtained in step S6 with a high-pressure homogenizer at 32MP, and filtering to obtain a homogenized solution;
s8: and (4) carrying out vacuum freeze drying on the homogenized liquid prepared in the step (S7) under the conditions of: the cold trap temperature is-31 ℃, the heating temperature is 33 ℃, the vacuum degree is 135Pa, and the materials are dried until the moisture content of the materials is 1.1 percent to prepare dry powder;
s9: quinoa pretreatment: weighing quinoa according to a weight part ratio, enriching gamma-aminobutyric acid (GABA) after the quinoa germinates, and then fermenting the germinated quinoa by utilizing lactic acid bacteria to further enrich GABA to prepare pretreated quinoa, wherein the lactic acid bacteria account for 5% of the weight of the germinated quinoa;
s10: adding the dry powder prepared in the step S8 and corn starch into the preprocessed quinoa prepared in the step S9 for compounding to prepare a compound material;
s11: and (5) drying, sterilizing and packaging the compound material prepared in the step (S10) to prepare the quinoa-litsea cubeba meal replacement porridge capable of being brewed quickly.
Comparative example 1
The preparation method is basically the same as that of the example 3, except that 4-O-alpha-D-glucopyranosyl-D-glucose, polyglycerol polyricinoleate and sodium alginate are absent in the raw materials for preparing the quinoa-red-litsea-vine meal-replacing porridge.
Comparative example 2
The preparation method is basically the same as that of the example 3, except that 4-O-alpha-D-glucopyranosyl-D-glucose is absent in the raw materials for preparing the quinoa-red litsea cubeba meal replacement porridge.
Comparative example 3
The preparation method is basically the same as that of example 3, except that the polyglycerol polyricinoleate is absent in the raw materials for preparing the quinoa-litsea cubeba meal replacement porridge.
Comparative example 4
The preparation method is basically the same as that of the example 3, except that sodium alginate is absent in the raw materials for preparing the quinoa-litsea cubeba meal replacement porridge.
First, homogeneous liquid centrifugal precipitation rate detection test
And (3) calculating the centrifugal precipitation rate of the homogeneous liquid prepared in the examples 1-3 and the comparative examples 1-4, specifically weighing 50g of each group of homogeneous liquid, putting the homogeneous liquid into a centrifugal tube, centrifuging at 6000r/min for 15min, discarding a floating elutriation layer and a middle whey layer, weighing the mass of the precipitate, repeating the steps for 3 times for each sample, and taking an average value. The centrifugation yield/%, which is 100% × precipitate mass/homogeneous mass, is shown in the following table:
remarking: the smaller the centrifugal precipitation rate, the less the precipitation rate, indicating the better the homogeneous liquid stability.
From the above table, it can be seen that: (1) the centrifugations of examples 1 to 3 showed the sedimenting rate of 0.78% to 0.91%, indicating that the homogenized liquid prepared by the present invention was excellent in stability.
(2) As can be seen from the data of example 3 and comparative examples 1-4, the absence of 4-O-alpha-D-glucopyranosyl-D-glucose, polyglycerol polyricinoleate, sodium alginate in the preparation of the homogenate according to the present invention all affects the centrifugation yield of the homogenate. From the centrifugation sedimentation rate data of example 3 and comparative examples 1 to 4, it was also calculated whether the use of 4-O- α -D-glucopyranosyl-D-glucose, polyglycerol polyricinoleate and sodium alginate resulted in a synergistic effect on the centrifugation sedimentation rate. The calculation is as follows:
1) the effect value of the centrifugal precipitation rate decreased when 4-O- α -D-glucopyranosyl-D-glucose, polyglycerol polyricinoleate and sodium alginate were used together is |2.08-0.78| 1.3 (%), which can be calculated from the centrifugal precipitation rate data of example 3 and comparative example 1.
2) The effect value of the centrifugal precipitation rate decreased when 4-O- α -D-glucopyranosyl-D-glucose was used alone, i.e., |1.13-0.78| 0.35 (%), can be calculated from the centrifugal precipitation rate data of example 3 and comparative example 2.
3) The effect value of the reduction of the centrifugal precipitation rate of polyglycerol ricinoleate when used alone is |1.05 to 0.78| 0.27 (%), which can be calculated from the centrifugal precipitation rate data of example 3 and comparative example 3.
4) The effect value of the reduction in the centrifugal precipitation rate when sodium alginate alone was used was |1.26 to 0.78| 0.48 (%), which can be calculated from the centrifugal precipitation rate data of example 3 and comparative example 4.
The effect value of the centrifugation sedimentation rate decreased by the addition of 4-O- α -D-glucopyranosyl-D-glucose, polyglycerol polyricinoleate, and sodium alginate when used alone was calculated to be 0.35+0.27+0.48 to 1.1 (%).
In summary, the effect value of the reduced centrifugal precipitation rate when 4-O- α -D-glucopyranosyl-D-glucose, polyglycerol ricinoleate, and sodium alginate are used together can be calculated to be higher than the effect value of the reduced centrifugal precipitation rate when 4-O- α -D-glucopyranosyl-D-glucose, polyglycerol ricinoleate, and sodium alginate are used alone respectively by the percentage of (1.3-1.1) ÷ 1.1 × 100% ═ 18.2% > 10%, since 18.2% is greater than 10%, which indicates that 4-O- α -D-glucopyranosyl-D-glucose, polyglycerol ricinoleate, and sodium alginate are used together to generate a synergistic effect and reduce the centrifugal precipitation rate synergistically, because:
4-O-alpha-D-glucopyranosyl-D-glucose can have high affinity with protein particles, and a sugar film can be formed on the surface of protein after the 4-O-alpha-D-glucopyranosyl-D-glucose is added, so that the affinity of the protein and a dispersion medium is improved, the protein is stably and uniformly dispersed in the homogeneous solution, and the centrifugal precipitation rate is further reduced; polyglycerol polyricinoleate molecules are directionally adsorbed towards the surface of water oil to form a stable complex, the adsorption capacity is increased, the surface tension is reduced, the aggregation among particles in homogeneous liquid is prevented, the formation of a sugar film on the surfaces of 4-O-alpha-D-glucopyranosyl-D-glucose and protein is promoted to be enhanced, and the centrifugal precipitation rate is reduced; the sodium alginate can inhibit protein particles in the homogeneous liquid from interacting to form colloid, so that the protein particles are in a negative charge state as a whole, and the homogeneous liquid provided by the invention is kept in a stable state under the repulsion action. Therefore, under the interaction of 4-O-alpha-D-glucopyranosyl-D-glucose, polyglycerol ricinoleate and sodium alginate, the centrifugal precipitation rate of the homogeneous liquid is synergistically reduced, the stability of the homogeneous liquid is improved, and the quality guarantee period is prolonged.
Second, enrichment GABA test after quinoa pretreatment
The results of the tests conducted on the GABA content enriched after quinoa pretreatment in examples 1-3 are shown in the following table.
From the above table, it can be seen that: after the raw quinoa is pretreated (germinated and fermented after germination), the GABA content is increased to over 199.41mg/100g, which shows that the nutrient content of the raw quinoa is greatly increased and the added value is increased after the raw quinoa is pretreated.
Third, uric acid lowering test
1. Molding liquid: adenine aqueous solution with concentration of 4 mg/mL; 1.2g/mL yeast extract aqueous solution.
2. Experimental animals, 18 Kunming mice, SPF grade, half and half of male and female, 19.5-21.7 g; 18 Kunming mice were acclimatized for one week and then randomly divided into treatment groups 1-3 of 6 mice each; and measuring and recording the initial blood uric acid value of each mouse after grouping, and counting the average value of the initial blood uric acid of each group of mice.
3. Establishing an animal model:
respectively performing intragastric perfusion on 18 Kunming mice by using molding solution twice a day, and continuously performing intragastric perfusion for 8 days to prepare a hyperuricemia model; in each gastric lavage, adenine solution is administered in an amount of 100ml/kg adenine, and yeast extract solution is administered in an amount of 30ml/kg yeast extract.
2 drops of tail peripheral blood of the mice are collected after the modeling is finished, a disposable chip and an instrument are used for rapidly measuring, the blood uric acid value of each group of mice after the modeling is finished is recorded, and the average value of the blood uric acid of each group of mice after the modeling is finished is counted.
4. Administration method and blood uric acid value test:
the following medicines are used for the mice in the treatment groups 1-3, and each mouse is fed with 1g of quinoa and litsea cubeba meal replacement porridge every day.
Treatment group 1: the quinoa-litsea cubeba meal replacement porridge of example 1;
treatment group 2: the quinoa-litsea cubeba meal replacement porridge of example 2;
treatment group 3: the quinoa-litsea cubeba meal replacement porridge of example 3.
And continuously feeding the litsea cubeba meal-replacing porridge for 2 days by each group of mice, measuring and recording blood uric acid values of the mice after drug effect test of each group after 2 days, counting blood uric acid average values of the mice after drug effect test of each group, comparing the initial blood uric acid average values, modeling blood uric acid average values and drug effect test blood uric acid average values, and obtaining results shown in the following table.
From the above table, it can be seen that: after the mice in the treatment groups 1-3 are fed with the quinoa red vine meal replacement porridge prepared in the examples 1-3, the average value of blood uric acid after modeling is 339.0-343.1 mu mol/L to 183.4-191.5 mu mol/L after 2 days, which shows that the quinoa red vine meal replacement porridge disclosed by the invention is excellent in uric acid reduction effect after short-term use.
The above description should not be taken as limiting the invention to the embodiments, but rather, as will be apparent to those skilled in the art to which the invention pertains, numerous simplifications or substitutions may be made without departing from the spirit of the invention, which shall be deemed to fall within the scope of the invention as defined by the claims appended hereto.
Claims (10)
1. The quinoa-litsea cubeba meal replacement porridge is characterized by comprising the following raw materials in parts by weight: 60-80 parts of quinoa, 25-45 parts of corn starch, 200 parts of litsea cubeba 150-.
2. The quinoa-litsea cubeba meal-replacing porridge according to claim 1, is characterized by comprising the following raw materials in parts by weight: 75 parts of quinoa, 40 parts of corn starch, 160 parts of litsea cubeba, 27 parts of dendrobium moniliforme, 12 parts of gynura divaricata, 18 parts of angelica sinensis leaves, 450 parts of water, 1.6 parts of vitamin C, 5 parts of 4-O-alpha-D-glucopyranosyl-D-glucose, 0.4 part of polyglycerol polyricinoleate and 1 part of sodium alginate.
3. The preparation method of the quinoa-litsea cubeba meal replacement porridge according to claim 1 or 2, which is characterized by comprising the following steps of:
s1: weighing the Siberian cocklebur stems, the dendrobium candidum, the gynura bicolor and the angelica leaves according to the weight part ratio, and then mixing the raw materials to obtain the mixed raw materials;
s2: placing the mixed raw materials obtained in the step S1 into water at 86-90 ℃ for blanching for 1-2min, taking out, placing into a color protection solution for color protection, and obtaining a color protection raw material;
s3: pulping the color protection raw material prepared in the step S2 to prepare a pulping material;
s4: adding complex enzyme into the pulped material prepared in the step S3, and performing enzymolysis for 1-1.5h at the temperature of 48-55 ℃ to prepare an enzymolysis material;
s5: grinding the enzymolysis material prepared in the step S4 in a colloid mill to prepare a ground material;
s6: adding auxiliary materials into the ground material prepared in the step S5, wherein the auxiliary materials comprise 500 parts of vitamin C, 4-O-alpha-D-glucopyranosyl-D-glucose, polyglycerol polyricinoleate, sodium alginate and water, and then blending for 8-10min at the rotation speed of 200-300r/min to prepare blending liquid;
s7: homogenizing the prepared solution obtained in the step S6 by using a homogenizer, and filtering to obtain a homogenized solution;
s8: and (4) carrying out vacuum freeze drying on the homogenized liquid prepared in the step (S7) under the conditions of: the temperature of the cold trap is between 28 ℃ below zero and 32 ℃ below zero, the heating temperature is between 31 ℃ and 34 ℃, the vacuum degree is 140Pa, and the materials are dried until the moisture content of the materials is less than or equal to 1.2 percent to prepare dry powder;
s9: quinoa pretreatment: weighing quinoa according to the weight part ratio, enriching gamma-aminobutyric acid (GABA) after the quinoa germinates, and fermenting the germinated quinoa by utilizing lactic acid bacteria to further enrich GABA to prepare pretreated quinoa;
s10: adding the dry powder prepared in the step S8 and corn starch into the preprocessed quinoa prepared in the step S9 for compounding to prepare a compound material;
s11: and (5) drying, sterilizing and packaging the compound material prepared in the step (S10) to prepare the quinoa-litsea cubeba meal replacement porridge capable of being brewed quickly.
4. The method for preparing the quinoa-litsea cubeba meal-replacing porridge according to claim 3, wherein the mass ratio of the mixed raw materials to the color-protecting liquid in step S2 is 1: 2-3.
5. The method for preparing the quinoa-litsea cubeba meal-replacing porridge according to claim 4, wherein the color-protecting solution is an aqueous solution containing 0.1-0.2% by mass of sodium erythorbate, 2.2-3% by mass of leucine, 1.3-1.8% by mass of amino-oligosaccharide and 0.2-0.4% by mass of sodium phytate.
6. The method for preparing the quinoa-litsea cubeba meal-replacing porridge according to claim 3, wherein the amount of the complex enzyme added to the pulping material in the step S4 is 0.3-0.5% of the total weight of the pulping material.
7. The method for preparing the quinoa-litsea cubeba meal-replacing porridge according to claim 6, wherein the complex enzyme consists of pectinase, alpha-amylase, papain and transglucosidase in a mass ratio of 6-9:3-5:1-2: 2-3.
8. The method for preparing the quinoa-litsea cubeba meal-replacing porridge according to claim 3, wherein in step S5, the enzymatic hydrolysate is put into a colloid mill and ground to the fineness of less than 20 μm to obtain a ground material.
9. The method of claim 3, wherein the step S7 is to homogenize the blended solution with a high pressure homogenizer at 20-35MP to obtain a homogenized solution.
10. The method for preparing the quinoa-litsea cubeba meal-replacing porridge according to claim 3, wherein the lactic acid bacteria account for 3% -6% of the weight of the germinated quinoa in step S9.
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