CN104109626B - A kind of sprout brown rice rich in selenium asparagus fermented vinegar and preparation method thereof - Google Patents
A kind of sprout brown rice rich in selenium asparagus fermented vinegar and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of preparation method of sprout brown rice rich in selenium asparagus fermented vinegar, adopt sprout brown rice rich in selenium and asparagus mixed fermentation, in fermenting process, add polygalacturonase, amylase, saccharifying enzyme, Rhizopus oryzae CICC3099, yeast saccharomyces cerevisiae CICC1050, Acetobacter pasteurianus CICC21870.Alpha-aminobutyric acid content 8-13mg/100g in obtained vinegar, vitamin contents more than 0.3%, selenium content 200ug/Kg.Therefore, the vinegar nutritive ingredient using the method to produce is many, local flavor sweet-smelling, and mouthfeel is soft sweet refreshing, is the vinegar based article simultaneously with desirable mouth feel and nutritional health function.
Description
Technical field
The invention belongs to health care edible vinegar manufacture field, be specifically related to a kind of sprout brown rice rich in selenium asparagus fermented vinegar and preparation method thereof.
Background technology
Vinegar can generate voluntarily in physical environment, and ancient times, the mankind just started edible vinegar from very early.In China, it has been generally acknowledged that vinegar starts when the Western Zhou Dynasty to be brewageed.The acetic acid of composition usually containing 3%-5% of vinegar, lactic acid, several mineral materials, what have also has a small amount of tartrate, citric acid etc.Vinegar has detumescence, detoxifies, helps digestion, effect of scattered silt, and drinking appropriate vinegar has larger help to healthy.
Asparagus formal name used at school reed bamboo shoot, its quality is fresh and tender, local flavor is delicious, tender good to eat, the trace element containing rich in protein, VITAMIN, mineral substance and needed by human body in asparagus stem, also the multiple steroid saponin material of distinctive asparagine is contained in addition in asparagus, all effective in cure to cardiovascular diseases, oedema, urocystitis, leukemia, also there is anticancer effect, the long-term edible useful taste of asparagus, have good result for the treatment of to human body numerous disease, thus asparagus is fabulous Dietotherapy health food.
Selenium, by the trace element as needed by human, has the good reputation of anticancer king.It has ten large effects: 1, improve body immunity; 2, anti-oxidant, delay senility; 3, Conservative restoration cell; 4, the treatment of diabetes is participated in; 5, cancer-resisting; 6, eyes are protected; 7, erythrocytic oxygen carrying capacity is improved; 8, cardiovascular and cerebrovascular diseases is prevented and treated; 9, removing toxic substances, gas defence, antipollution; 10, liver is protected.At present,
chinese Soclety of Nutritionadult's intake of recommending is 50-250 microgram every day, and China's 2/3 regional selenium intake is lower than minimum recommended value, and therefore, Chinese generally lack selenium.Given this, rich selenium in daily edible vinegar, effectively improves the intake of selenium.
Containing γ-aminobutyric acid (GABA) in sprout brown rice, it is a kind of naturally occurring nonprotein amino acid, is that inhibitory nerve important in mammalian central nervous system passes on material, has regulating effect to the several functions of body.When GABA in human body lacks, anxiety, uneasiness, the mood such as tired, worried can be produced, need to supplement to releive mood in time.It is approved as new resource food by health ministry, and have calm neural, anxiety, reduces blood pressure, and reduces blood ammonia, improves brain vigor, numerous effect such as to release the pressure.
Now, in order to increase the health-care effect of vinegar, existing correlative study person develops health vinegar, such as: the Chinese Patent Application No. CN201010187965.8 " producing and manufacturing technique of papaya ester
", Chinese Patent Application No. CN201210162462.4 " a kind of tea vinegar beverage and preparation method thereof
"etc..What study is all the health vinegar with traditional method preparation with effect in a certain respect, and composition is comparatively single, and mouthfeel is general, and the vinegar based article simultaneously with desirable mouth feel and nutritional health function is less.
Summary of the invention
The present invention is directed to the deficiency of above-mentioned existence, adopt sprout brown rice rich in selenium and asparagus mixed fermentation, during the fermentation, nutritive and health protection components in asparagus and sprout brown rice rich in selenium is incorporated in vinegar, both mouthfeel and the local flavor of vinegar had been improved, enhance again the health-care effect of sprout brown rice rich in selenium asparagus fermented vinegar, thus the health-care effect of vinegar and quality are significantly promoted.
In order to realize object of the present invention, the present inventor is studied and persistent exploration by lot of experiments, finally obtains following technical scheme:
A kind of sprout brown rice rich in selenium asparagus fermented vinegar, is characterized in that: described sprout brown rice rich in selenium asparagus fermented vinegar is prepared from by following weight primarily of following raw material: asparagus 40-50kg, water 300-350kg, brown rice 80-120kg.
The preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar of the present invention, comprises the following steps:
(1) preparation of asparagus enzymolysis solution: get fresh, cleaned asparagus 40-50kg, colloidal mill defibrination is used after being cut into segment, water 300-350kg is added in slurries, the polygalacturonase 0.70-0.90g/kg adding enzyme 6000U/g alive with the weight ratio (g/kg) of raw material is pressed after mixing, under 45-50 DEG C of condition, enzymolysis 14-18h, obtains enzymolysis solution;
(2) saccharification of sprout brown rice rich in selenium slurries: by 80-120kg sprout brown rice rich in selenium colloidal mill defibrination, then the enzymolysis solution obtained with step (1) mixes, press the amylase 0.60-0.90g/kg adding enzyme 9000U/g alive with the weight ratio (g/kg) of raw material, the saccharifying enzyme 0.40-0.70g/kg of enzyme 10000U/g alive, saccharification 9-12h under 31-36 DEG C of condition;
(3) distiller's wort preparation: in the saccharified liquid that step (2) obtains, by adding Rhizopus oryzae 0.45-0.55g/kg, yeast saccharomyces cerevisiae 0.75-0.90g/kg with the weight ratio (g/kg) of raw material, being placed in 35-40 DEG C of condition bottom fermentation and obtaining distiller's wort in 10-13 days;
(4) distiller's wort solid state fermentation: in the distiller's wort that step (3) obtains, by adding Acetobacter pasteurianus CICC218700.70-0.90g/kg with the weight ratio (g/kg) of raw material, adopting solid state fermentation 7-10 days under 37-42 DEG C of condition, obtaining fermented liquid;
(5) sprout brown rice rich in selenium asparagus fermented vinegar preparation: fermented liquid is centrifugal, filters, sterilizing, and packaging, can obtain sprout brown rice rich in selenium asparagus fermented vinegar.
Preferably, the preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar as above, wherein the sprout brown rice rich in selenium preparation method described in step (2) is: wrap brown rice with gauze, 15-25h is soaked in 2-8 mole/L growth hormone solution, then be placed in 25-35 DEG C of constant incubator and carry out cultivation and germinate, and every 4h spray once rich selenium flushing liquor, until bud grow to 1-2cm long time, take out to clean and drain, obtain sprout brown rice rich in selenium.
Preferably, the preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar as above, it is characterized in that the preparation method of rich selenium flushing liquor is: the rich selenium coal gangue pulverizing article of cut-off footpath between 60-100 order, be placed in 20-30%(V/V) sulfurous acid solution soak 20-50h, use ultrasonic vibration simultaneously, accelerate the dissolving of selenium element; Add 5-10%(V/V after immersion) sodium radio-phosphate,P-32 solution, precipitate the heavy metal in selenium ore, filter, in filtered liquid, add concentration is 8-13%(V/V) sodium hydroxide solution, by the pH regulator of filtered liquid to 6.5-8.5, i.e. obtained flushing liquor.
Preferably, the preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar as above, wherein the Rhizopus oryzae described in step (3) is Rhizopus oryzae CICC3099, yeast saccharomyces cerevisiae is yeast saccharomyces cerevisiae CICC1050.
Preferably, the preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar as above, wherein the Acetobacter pasteurianus described in step (4) is Acetobacter pasteurianus CICC21870.
The present invention is compared with existing vinegar processing technology, and tool has the following advantages:
(1) adopt solid fermentating mode, what utilize sprout brown rice rich in selenium to produce enriches enzyme system, more abundant, the obtained vinegar nutritive ingredient of raw material glycolysis is increased, local flavor sweet-smelling, and mouthfeel is soft sweet refreshing, and quality is improved.
(2) green plants asparagus and sprout brown rice rich in selenium mixing is utilized to produce vinegar, what produced by asparagus enzymolysis enriches VITAMIN, amino acid, the nutritive and health protection components such as trace element and sprout brown rice rich in selenium are dissolved in vinegar, finally alpha-aminobutyric acid content in the vinegar obtained can be made to reach 8-12mg/100g, vitamin contents reaches 0.3%, selenium content 200ug/Kg, thus it is safe and reliable to make the vinegar obtained reach both nutrient healths, there is again good health-care effect.
Embodiment
Be below specific embodiments of the invention, technical scheme of the present invention is done to describing further, but protection scope of the present invention be not limited to these embodiments.Every do not deviate from the present invention's design change or equivalent substituting include within protection scope of the present invention.
embodiment 1
(1) preparation of asparagus enzymolysis solution: get fresh, cleaned asparagus 40kg, colloidal mill defibrination is used after being cut into segment, water 300kg is added in slurries, the polygalacturonase 0.70g/kg adding enzyme 6000U/g alive with the weight ratio (g/kg) of raw material is pressed after mixing, under 45 DEG C of conditions, enzymolysis 14h, obtains enzymolysis solution;
(2) preparation of liquid is shone in the spray of rich selenium: the rich selenium coal gangue pulverizing article of cut-off footpath 60 object, is placed in 20%(V/V) sulfurous acid solution soak 20h, use ultrasonic vibration simultaneously, accelerate the dissolving of selenium element; Add 5%(V/V after immersion) sodium radio-phosphate,P-32 solution, precipitate the heavy metal in selenium ore, filter, in filtered liquid, add concentration is 8%(V/V) sodium hydroxide solution, by the pH regulator to 6.5 of filtered liquid, i.e. obtained flushing liquor;
(3) preparation of sprout brown rice rich in selenium:: wrap brown rice with gauze, 15h is soaked in 2 moles/L growth hormone solution, then be placed in 25 DEG C of constant incubators and carry out cultivation germination, and every 4h spray once rich selenium flushing liquor, until bud grow to 1cm long time, take out to clean and drain, obtain sprout brown rice rich in selenium;
(4) saccharification of sprout brown rice rich in selenium slurries: by 80kg sprout brown rice rich in selenium colloidal mill defibrination, then the enzymolysis solution obtained with step (1) mixes, press the amylase 0.60g/kg adding enzyme 9000U/g alive with the weight ratio (g/kg) of raw material, the saccharifying enzyme 0.40g/kg of enzyme 10000U/g alive, saccharification 9h under 31 DEG C of conditions;
(5) distiller's wort preparation: in the saccharified liquid that step (2) obtains, by adding Rhizopus oryzae 0.45g/kg, yeast saccharomyces cerevisiae 0.75g/kg with the weight ratio (g/kg) of raw material, being placed in 35 DEG C of condition bottom fermentations and obtaining distiller's wort in 10 days;
(6) distiller's wort solid state fermentation: in the distiller's wort that step (3) obtains, by adding Acetobacter pasteurianus 0.70g/kg with the weight ratio (g/kg) of raw material, adopting solid state fermentation 7 days, obtaining fermented liquid under 37 DEG C of conditions;
(7) sprout brown rice rich in selenium asparagus fermented vinegar preparation: fermented liquid is centrifugal, filters, sterilizing, and packaging, can obtain sprout brown rice rich in selenium asparagus fermented vinegar.
embodiment 2
(1) preparation of asparagus enzymolysis solution: get fresh, cleaned asparagus 50kg, colloidal mill defibrination is used after being cut into segment, water 350kg is added in slurries, the polygalacturonase 0.90g/kg adding enzyme 6000U/g alive with the weight ratio (g/kg) of raw material is pressed after mixing, under 50 DEG C of conditions, enzymolysis 18h, obtains enzymolysis solution;
(2) preparation of liquid is shone in the spray of rich selenium: the rich selenium coal gangue pulverizing article of cut-off footpath 100 object, is placed in 30%(V/V) sulfurous acid solution soak 50h, use ultrasonic vibration simultaneously, accelerate the dissolving of selenium element; Add 10%(V/V after immersion) sodium radio-phosphate,P-32 solution, precipitate the heavy metal in selenium ore, filter, in filtered liquid, add concentration is 13%(V/V) sodium hydroxide solution, by the pH regulator to 8.5 of filtered liquid, i.e. obtained flushing liquor;
(3) preparation of sprout brown rice rich in selenium: wrap brown rice with gauze, 25h is soaked in 8 moles/L growth hormone solution, then be placed in 35 DEG C of constant incubators and carry out cultivation germination, and every 4h spray once rich selenium flushing liquor, until bud grow to 2cm long time, take out to clean and drain, obtain sprout brown rice rich in selenium;
(4) saccharification of sprout brown rice rich in selenium slurries: by 120kg sprout brown rice rich in selenium colloidal mill defibrination, then the enzymolysis solution obtained with step (1) mixes, press the amylase 0.90g/kg adding enzyme 9000U/g alive with the weight ratio (g/kg) of raw material, the saccharifying enzyme 0.70g/kg of enzyme 10000U/g alive, saccharification 12h under 36 DEG C of conditions;
(5) distiller's wort preparation: in the saccharified liquid that step (2) obtains, by adding Rhizopus oryzae 0.55g/kg, yeast saccharomyces cerevisiae 0.90g/kg with the weight ratio (g/kg) of raw material, being placed in 40 DEG C of condition bottom fermentations and obtaining distiller's wort in 13 days;
(6) distiller's wort solid state fermentation: in the distiller's wort that step (3) obtains, by adding Acetobacter pasteurianus 0.90g/kg with the weight ratio (g/kg) of raw material, adopting solid state fermentation 10 days, obtaining fermented liquid under 42 DEG C of conditions;
(7) sprout brown rice rich in selenium asparagus fermented vinegar preparation: fermented liquid is centrifugal, filters, sterilizing, and packaging, can obtain sprout brown rice rich in selenium asparagus fermented vinegar.
embodiment 3
(1) preparation of asparagus enzymolysis solution: get fresh, cleaned asparagus 45kg, colloidal mill defibrination is used after being cut into segment, water 325kg is added in slurries, the polygalacturonase 0.80g/kg adding enzyme 6000U/g alive with the weight ratio (g/kg) of raw material is pressed after mixing, under 47 DEG C of conditions, enzymolysis 16h, obtains enzymolysis solution;
(2) preparation of liquid is shone in the spray of rich selenium: the rich selenium coal gangue pulverizing article of cut-off footpath 80 object, is placed in 25%(V/V) sulfurous acid solution soak 35h, use ultrasonic vibration simultaneously, accelerate the dissolving of selenium element; Add 7%(V/V after immersion) sodium radio-phosphate,P-32 solution, precipitate the heavy metal in selenium ore, filter, in filtered liquid, add concentration is 10%(V/V) sodium hydroxide solution, by the pH regulator to 7.5 of filtered liquid, i.e. obtained flushing liquor.
(3) preparation of sprout brown rice rich in selenium: wrap brown rice with gauze, 20h is soaked in 5 moles/L growth hormone solution, then be placed in 30 DEG C of constant incubators and carry out cultivation germination, and every 4h spray once rich selenium flushing liquor, until bud grow to 1.5cm long time, take out to clean and drain, obtain sprout brown rice rich in selenium.
(4) saccharification of sprout brown rice rich in selenium slurries: by 100kg sprout brown rice rich in selenium colloidal mill defibrination, then the enzymolysis solution obtained with step (1) mixes, press the amylase 0.75g/kg adding enzyme 9000U/g alive with the weight ratio (g/kg) of raw material, the saccharifying enzyme 0.50g/kg of enzyme 10000U/g alive, saccharification 11h under 34 DEG C of conditions;
(5) distiller's wort preparation: in the saccharified liquid that step (2) obtains, by adding Rhizopus oryzae 0.50g/kg, yeast saccharomyces cerevisiae 0.85g/kg with the weight ratio (g/kg) of raw material, being placed in 38 DEG C of condition bottom fermentations and obtaining distiller's wort in 12 days;
(6) distiller's wort solid state fermentation: in the distiller's wort that step (3) obtains, by adding Acetobacter pasteurianus 0.80g/kg with the weight ratio (g/kg) of raw material, adopting solid state fermentation 9 days, obtaining fermented liquid under 40 DEG C of conditions;
(7) sprout brown rice rich in selenium asparagus fermented vinegar preparation: fermented liquid is centrifugal, filters, sterilizing, and packaging, can obtain sprout brown rice rich in selenium asparagus fermented vinegar.
comparative example 1:
The supplementary material kind that this embodiment uses with embodiment 1, quantity of adding, order of addition and the fermentation condition in each stage are all identical, only in comparative example, do not carry out selenium-rich germinated process to brown rice.
comparative example 2:
Do not use the enzymolysis solution of asparagus in this embodiment, namely lack the step (1) in example 2, all the other steps are all identical with example 2.
comparative example 3:
The difference of this embodiment and example 3 is steps (1) in comparative example 3, do not add zymin in (4), and other are all identical with example 3.
Asparagus vinegar prepared by embodiment 1-3 and comparative example 1-3, its attributional analysis is as following table:
Table 1 each embodiment attributional analysis table
From above data, use asparagus vinegar prepared by the present invention, VITAMIN, protein, amino acid and enzyme equal size are all higher than comparison example, especially selenium-rich germinated process is not carried out to brown rice in comparative example 1, r-Gamma-propalanine content is 0, selenium content is 0, does not use asparagus raw material in comparison example 2, and in the vinegar of preparation, vitamin contents is extremely low.Therefore, using the sprout brown rice rich in selenium asparagus fermented vinegar prepared of the present invention to make full use of, the glycolysis such as sprout brown rice rich in selenium and asparagus produces enriches enzyme system, VITAMIN and macroelement, trace element, obtained vinegar nutritive ingredient is many, local flavor sweet-smelling, mouthfeel is soft sweet refreshing, is the vinegar based article simultaneously possessing splendid mouthfeel and nutritional health function.
Claims (5)
1. a preparation method for sprout brown rice rich in selenium asparagus fermented vinegar, is characterized in that the method comprises the following steps:
(1) preparation of asparagus enzymolysis solution: get fresh, cleaned asparagus 40-50kg, colloidal mill defibrination is used after being cut into segment, water 300-350kg is added in slurries, add the polygalacturonase 0.70-0.90g/kg of enzyme 6000U/g alive by the weight ratio with raw material after mixing, under 45-50 DEG C of condition, enzymolysis 14-18h, obtains enzymolysis solution;
(2) saccharification of sprout brown rice rich in selenium slurries: by 80-120kg sprout brown rice rich in selenium colloidal mill defibrination, then the enzymolysis solution obtained with step (1) mixes, the amylase 0.60-0.90g/kg of enzyme 9000U/g alive is added by the weight ratio with raw material, the saccharifying enzyme 0.40-0.70g/kg of enzyme 10000U/g alive, saccharification 9-12h under 31-36 DEG C of condition;
(3) distiller's wort preparation: in the saccharified liquid that step (2) obtains, add Rhizopus oryzae 0.45-0.55g/kg, yeast saccharomyces cerevisiae 0.75-0.90g/kg by the weight ratio with raw material, is placed in 35-40 DEG C of condition bottom fermentation and obtains distiller's wort in 10-13 days;
(4) distiller's wort solid state fermentation: in the distiller's wort that step (3) obtains, add Acetobacter pasteurianus 0.70-0.90g/kg by the weight ratio with raw material, adopt solid state fermentation 7-10 days under 37-42 DEG C of condition, obtain fermented liquid;
(5) sprout brown rice rich in selenium asparagus fermented vinegar preparation: fermented liquid is centrifugal, filters, sterilizing, and packaging, can obtain sprout brown rice rich in selenium asparagus fermented vinegar.
2. the preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar according to claim 1, it is characterized in that: described in step (2), the preparation method of sprout brown rice rich in selenium is: wrap brown rice with gauze, 15-25h is soaked in 2-8 mole/L growth hormone solution, then be placed in 25-35 DEG C of constant incubator and carry out cultivation germination, and every 4h spray once rich selenium flushing liquor, until bud grow to 1-2cm long time, take out clean drains, obtain sprout brown rice rich in selenium.
3. the preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar according to claim 2, it is characterized in that the preparation method of rich selenium flushing liquor is: the rich selenium coal gangue pulverizing article of cut-off footpath between 60-100 order, be placed in 20-30%(V/V) sulfurous acid solution soak 20-50h, use ultrasonic vibration simultaneously, accelerate the dissolving of selenium element; Add 5-10%(V/V after immersion) sodium radio-phosphate,P-32 solution, precipitate the heavy metal in selenium ore, filter, in filtered liquid, add concentration is 8-13%(V/V) sodium hydroxide solution, by the pH regulator of filtered liquid to 6.5-8.5, i.e. obtained flushing liquor.
4. the preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar according to claim 1, is characterized in that: the Rhizopus oryzae described in step (3) is Rhizopus oryzae CICC3099, yeast saccharomyces cerevisiae is yeast saccharomyces cerevisiae CICC1050.
5. the preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar according to claim 1, is characterized in that: the Acetobacter pasteurianus described in step (4) is Acetobacter pasteurianus CICC21870.
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| CN105462796A (en) * | 2015-11-24 | 2016-04-06 | 全椒井府富硒生态牧业有限公司 | Selenium-rich vinegar |
| CN105567544A (en) * | 2016-02-06 | 2016-05-11 | 湖北工业大学 | Garlic juice and mulberry vinegar |
| CN108220076A (en) * | 2018-04-18 | 2018-06-29 | 洪瑞茜 | A kind of selenium-rich germinated Rough glutinous rice red hayberry wine and preparation method thereof |
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Effective date of registration: 20201119 Address after: No.8, yangqueba group, yangqueba village, wuyangba sub district office, Enshi Tujia and Miao Autonomous Prefecture, Hubei Province Patentee after: ENSHI XISHENG PLANT TECHNOLOGY Co.,Ltd. Address before: 430068 Wuchang City, Wuhan Province, South Lake, Li Jia Dun village, No. 1, No., No. 1 Patentee before: HUBEI University OF TECHNOLOGY |
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