CN114032246B - Rice light harvesting pigment chlorophyll a/b binding protein gene Lhcb3 and application thereof in rice photoprotection - Google Patents
Rice light harvesting pigment chlorophyll a/b binding protein gene Lhcb3 and application thereof in rice photoprotection Download PDFInfo
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/8269—Photosynthesis
Abstract
The invention discloses a rice light-harvesting pigment chlorophyll a/b binding protein geneLhcb3The nucleotide sequence of the rice light-harvesting pigment chlorophyll a/b binding protein gene is shown as a sequence table SEQ ID NO. 1Lhcb3The experiment proves that the light-emitting diode is applied to the photoprotection of riceLhcb3Can reduce chlorophyll content, influence photosynthetic efficiency and NPQ value, the gene has important value in rice high light efficiency breeding and photoprotection, and provides reference for the gene to regulate other plant NPQ value.
Description
Technical Field
The invention belongs to the technical field of bioengineering, and particularly relates to a rice light-harvesting pigment chlorophyll a/b binding protein gene Lhcb3 and application thereof in rice photoprotection.
Background
The rice is used as one of three large grain crops in the world, is also an important grain crop in China, and the demand of the rice is increased due to the large increase of the population in the world. Light is essential for photosynthesis in plants, and chloroplast pigments in the photosynthetic machinery of plants absorb light energy and convert it into stable chemical energy. However, in the case of excess light energy, the plants receive more energy than they can convert, and if the excess light energy is not dissipated in time, the excess light can cause a light inhibition phenomenon, thereby reducing photosynthetic efficiency. Photosynthesis and its complexity, the coordination of which is mainly represented by a balance of absorption and utilization of light energy. Through long-term evolution, rice has formed a variety of protection mechanisms that coordinate the absorption and utilization of light energy. The non-photochemical quenching (NPQ) is an important protector, which can enable plants to dissipate absorbed surplus light energy in a harmless heat energy mode, can reduce the generation of active oxygen, and can effectively avoid the occurrence of photoinhibition.
Plants have two light systems (light system I and light system II) involved in the capture and conversion of light. Both consist of a core complex that undergoes a photochemical reaction and an antenna system that participates in light capture, which is a multiprotein super complex. The antennas of the plant's optical system II include smaller light harvesting complexes II (LHCB 4, LHCB5 and LHCB 6) and larger antenna complex trimers (LHCB 1, LHCB2 and LHCB 3), which are the most abundant membrane proteins on earth. The LHCB not only participates in capturing optical energy, but also participates in energy dissipation of excessive optical energy, and energy distribution of a conversion optical system I and an optical system II plays a role in optical protection.
Disclosure of Invention
The invention aims to provide a rice light-harvesting pigment chlorophyll a/b binding protein gene Lhcb3 and application thereof in rice photoprotection, a gene Lhcb3 for controlling chlorophyll content, photosynthetic rate and NPQ value is separated from rice, RNA interference expression is carried out on the gene, the gene is found to be capable of reducing chlorophyll content, influencing photosynthetic efficiency and NPQ value, and the gene has important value in rice high-light-efficiency breeding and photoprotection.
The invention is realized by the following technical scheme:
the invention provides a rice light harvesting pigment chlorophyll a/b binding protein gene Lhcb3, the nucleotide sequence of which is shown in a sequence table SEQ ID NO:1, which is obtained by cloning a light harvesting pigment chlorophyll a/b binding protein gene from a typical rice japonica rice variety Zhonghua 11, and then interfering the Lhcb3 gene by using RNAi interference technology, thus obtaining a transgenic plant with the Lhcb3 gene function inhibited.
The sequence table SEQ ID NO. 1 is the DNA sequence of the Lhcb3 gene cloned from flower 11 in rice variety, and the gene has the advantages of full length, long coding region and coding-amino acid.
T for inhibiting expression of Lhcb3 gene 0 Systematic investigation is carried out on chlorophyll content and photosynthetic functional period of the transgenic plant, and compared with a wild type, the material for inhibiting expression of the Lhcb3 gene is found that the SPAD value, chlorophyll a, chlorophyll b, total chlorophyll content and NPQ of the material are extremely obviously reduced; the chlorophyll a/chlorophyll b ratio rises.
For the Lhcb3 geneInhibition of expressed T 0 Co-segregation detection is carried out on the transgenic plant, and the fact that the plant with low LHCB3 gene expression level has low chlorophyll content and NPQ value content, and the phenotype and expression level of the chlorophyll content and the NPQ value are well co-segregated is found.
T for inhibiting expression of Lhcb3 gene 1 The transgenic plant is observed by a transmission electron microscope in the seedling stage, and the compactness of the lamellar structure of the thylakoid is lower than that of the wild type plant when the positive plant is compared with the wild type plant.
T for inhibiting expression of Lhcb3 gene 1 Systematic studies on the chlorophyll content and photosynthetic functional period of the system are carried out in the seedling stage of the transgenic plant, and the SPAD value, the chlorophyll a, the chlorophyll b and the total chlorophyll content are extremely obviously reduced, and the ratio of the chlorophyll a to the chlorophyll b is increased.
T for inhibiting expression of Lhcb3 gene 1 The plant heading period of the transgenic plant is systematically examined for the chlorophyll content and photosynthetic functional period of the system, and the SPAD value, the chlorophyll a, the chlorophyll b, the total chlorophyll content and the NPQ value are extremely obviously reduced, the ratio of the chlorophyll a to the chlorophyll b is increased, and the net photosynthetic rate is reduced.
T for inhibiting expression of Lhcb3 gene 1 The expression quantity of the transgenic plant in the seedling stage and the heading stage is detected and analyzed, and the expression quantity of the genes Lhcb1 and Lhcb2 related to the optical system II is drastically reduced after the expression quantity of the gene Lhcb3 is reduced in the seedling stage and the heading stage; the expression level of the genes Lhcb4, lhcb5 and Lhcb6 related to the optical system I is slightly reduced; the decrease in expression level of the gene PsbS1 related to photoprotection occurs in the heading stage, which indicates that Lhcb3 plays an important role in controlling photoprotection.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, the RNAi interference technology is used for inhibiting the rice light-harvesting pigment chlorophyll a/b binding protein gene OsLhcb3, so that a transgenic plant is obtained, physiological phenotypes of the transgenic plant and a wild photosynthetic functional period are systematically inspected and analyzed, and experiments prove that the Lhcb3 gene controls phenotypes such as chlorophyll content, photosynthetic rate, qE-type NPQ and the like, and the gene plays an important role in rice photoprotection.
2. The invention discovers that Lhcb3 can regulate and control NPQ value in rice for the first time, influences photosynthetic yield of the rice, and fully confirms the point by using transgenic materials. The gene plays an important role in rice photoprotection and plays an important role in rice high-light-efficiency breeding. The invention also enriches the functional knowledge of the Lhcb3 gene and provides a reference for regulating and controlling the NPQ value of other plants by the gene.
Drawings
Fig. 1: lhcb3 gene RNAiT 0 Chlorophyll content, NPQ value and expression quantity of transgenic plant;
fig. 2: lhcb3 gene RNAiT 1 SPAD values of two family seedling stages and heading stage of transgenic plants;
fig. 3: lhcb3 gene RNAiT 1 Chlorophyll content of the transgenic plants in the seedling stage and the heading stage of the two families;
fig. 4: lhcb3 gene RNAiT 1 Fv/Fm values of transgenic plants;
fig. 5: lhcb3 gene RNAiT 1 NPQ value of transgenic plants;
fig. 6: lhcb3 gene RNAiT 1 Net photosynthetic rate of transgenic plants;
fig. 7: she Setu of the Lhcb3 gene RNAi transgenic plant;
fig. 8: leaf transmission electron microscopy of Lhcb3 gene RNAi transgenic plants;
fig. 9: graph of relative expression level of Lhcb1.1 gene in Lhcb3 RNAi transgenic plants;
fig. 10: graph of relative expression level of Lhcb1.2 gene in Lhcb3 RNAi transgenic plants;
fig. 11: graph of relative expression level of Lhcb1.3 gene in Lhcb3 RNAi transgenic plants;
fig. 12: graph of relative expression level of Lhcb2 gene in Lhcb3 RNAi transgenic plants;
fig. 13: a graph of the relative expression level of the Lhcb3 gene in the Lhcb3 RNAi transgenic plant;
fig. 14: graph of relative expression level of Lhcb4 gene in Lhcb3 RNAi transgenic plants;
fig. 15: graph of relative expression level of Lhcb5 gene in Lhcb3 RNAi transgenic plants;
fig. 16: graph of relative expression level of Lhcb6 gene in Lhcb3 RNAi transgenic plants;
fig. 17: a graph of the relative expression level of the Lhca1 gene in the Lhcb3 RNAi transgenic plant;
fig. 18: a graph of the relative expression level of the Lhca3 gene in the Lhcb3 RNAi transgenic plant;
fig. 19: a graph of the relative expression level of the Lhca4 gene in the Lhcb3 RNAi transgenic plant;
fig. 20: a graph of the relative expression level of the Lhca6 gene in the Lhcb3 RNAi transgenic plant;
fig. 21: graph of the relative expression level of the PsbS1 gene in Lhcb3 RNAi transgenic plants.
Detailed Description
Example 1
Construction of Lhcb3 Gene RNAi vector
Extraction of DNA
Extracting DNA from young leaves of flowers 11 in typical japonica rice varieties; specifically, young leaves with the length of 2cm are taken from rice plants, placed into a 2mL centrifuge tube, added with 800 mu L of 1.5x CTAB, ground, subjected to water bath at 65 ℃ for 30min, and uniformly mixed every 10min in an upside down way. In a fume hood, adding 600 μl of chloroform into each tube of sample, shaking on a shaker for 30min, centrifuging at 8000rpm/min for 10min, sucking 400 μl of supernatant into a new centrifuge tube, adding 1000 μl of glacial ethanol, mixing, and standing at-20deg.C overnight; the next day, the mixture was centrifuged at 12000rpm/min for 15min. Pouring out the liquid, and reversely buckling the liquid on the water absorbing paper to absorb the surface liquid. Adding 1mL of 75% ethanol, mixing, standing for 2min, pouring out the liquid, reversely buckling on absorbent paper to suck the surface liquid, drying the liquid on a super clean bench, adding 200 μL of sterilized ddH 2 O dissolves DNA.
PCR amplification
PCR with the extracted DNA as template (amplification primer designed inside the second exon of Lhcb3 gene): 20 uL System (3 uL ddH) 2 O+2. Mu.L template+15. Mu.L mixture) +20. Mu.L mineral oil 15. Mu.L mixture is as follows:
pre-denaturing at 94℃for 5min; denaturation at 94℃for 30s, annealing at 58℃for 30s, extension at 72℃for 1min, cycle 32; PCR program amplification by extension at 72℃for 7min
Construction of RNAi vector
And (3) carrying out DNA gel cutting recovery on the amplified product of the PCR, connecting the recovered product to a T-easy vector through T4 DNA ligase, transferring the recombined vector to DH5 alpha escherichia coli strain through a heat shock method, and extracting the recombined plasmid Lhcb 3-T-easy. The recombinant plasmid Lhcb3-T-easy and RNAi vector pDS1301 are digested with BamH I and Kpn I, the digested product is connected to the digested pDS1301, the digested product is transferred into DH5 alpha E.coli strain by a heat shock method, the strain is cultured for 16 hours at 37 ℃, and a mutation-free monoclonal is selected for amplification and propagation, and the corresponding plasmid is extracted and named as Lhcb3-pDS1301-A. The recombinant plasmid Lhcb3-T-easy and RNAi vector Lhcb3-pDS1301-A are digested with SacI and SpeI, transferred into DH5 alpha E.coli strain by heat shock method, cultured for 16 hours at 37 ℃, the mutation-free monoclonal is selected for amplification and propagation, and the corresponding plasmid is extracted, named Lhcb3-RNAi-pDS1301. Thus, the Lhcb3 gene RNAi vector was constructed successfully. The constructed vector is sent to WU Han dynasty biological technology Co., ltd for genetic transformation, RNAi vector is integrated into rice genome through agrobacterium-mediated genetic transformation, and 12 transgenic seedlings are successfully obtained.
Test example 1 chlorophyll content measurement
Chlorophyll relative content determination: chlorophyll meter (SPAD meter) living body measurement method
The invention utilizes the chlorophyll meter to carry out living body measurement on the seedling stage and the heading stage of the rice, the SPAD value represents the relative value of the chlorophyll content of plant leaves, and the larger the value is, the higher the chlorophyll content is.
Absolute chlorophyll content determination-in-vitro determination method
The method for investigating the chlorophyll content in vitro comprises the following steps: after the selected mutant and the wild type leaf are placed into a centrifuge tube (5 plants are selected for sampling in each family), the leaf is immediately placed into an ice-filled foam box, and the leaf is immediately brought back into a laboratory after being wrapped by black cloth. Accurately weighing by an analytical balance, shearing, and adding acetone: ethanol: and (3) extracting the mixed extract of water (4.5:4.5:1) at 4 ℃ in a dark place, shaking the sample at intervals, measuring the absorbance values of the sample at 663nm and 645nm on a spectrophotometer after the sample is completely decolorized, and calculating the chlorophyll content according to a formula.
The specific calculation formula is as follows:
Chla=(12.71A663-2.59A645)×V/1000W
Chlb＝(22.88A645-4.67A663)×V/1000W
Total chl=(8.04A663+20.29A645)×V/1000W
wherein A663 and A645 are light absorption values under corresponding wavelengths respectively, V is the volume (ml) of the extract, W is weight g, and the unit of chlorophyll after conversion is mg/g.
By systematically examining the chlorophyll content of transgenic plants in seedling stage and heading stage, positive plants (mut) are found to have lower chlorophyll content than wild plants (WT), and Lhcb3 gene can down regulate the chlorophyll content, thus playing an important role in regulating the chlorophyll content.
Test example 2 determination of chlorophyll fluorescence
According to the invention, chlorophyll fluorescence measurement is carried out on the transgenic family by using a portable chlorophyll fluorescence modulation instrument PAM-2500, and statistical analysis is carried out on data after measurement. These plants were dark adapted for 30min prior to the assay. The minimal fluorescence of the open PSII center was measured by light measurement (F o ) After adaptation in the dark for 30min, the maximum fluorescence (F) of the closed PSII center was determined after application of a 0.8s pulse under saturated light m )。PSII(F v /F m ) Is defined as (F) m -F o )/F m . Measurement of steady state chlorophyll fluorescence (F) using actinic light (Red light) s ). In the light adaptation state F' m Measured by applying saturation pulses, F' o Then far-red light measurement is applied by turning off the light 2s after the saturation pulse. NPQ is defined as F m /F’ m -1；PSII( Φ PSII) actual quantum efficiencyThe rate is defined as (F' m -F s )/F’ m The method comprises the steps of carrying out a first treatment on the surface of the Photochemical quenching (q) p ) Is defined as 1- (F) s –F’ o )/(F’ m –F’ o )。
F of transgenic plant seedling stage and heading stage is inspected by a system v /F m And NPQ, positive plants (mut) were found to have lower F than wild-type plants (WT) v /F m And NPQ value, lhcb3 gene can down regulate F v /F m And NPQ value, plays an important role in regulating and controlling non-photochemical quenching and photoprotection of rice.
Test example 3 net photosynthetic rate measurement
The invention selects to measure the net photosynthetic rate of the transgenic family on a sunny day, and sets the gradient of the light intensity during measurement. And carrying out statistical analysis on the obtained data. A red/blue LED light source (6400-02B; li-Cor) was mounted on the leaf of rice, and the net photosynthetic rate (Pn) and the light response curve were determined using an LI-6400XT photosynthetic-fluorometer from Beijing Liper high technology Co. All measurements were at a temperature of 30℃and CO 2 Concentration of 400. Mu.LL -1 The process is carried out under the condition that the ambient humidity is 75+/-5 percent. During Pn measurement, photosynthetic Photon Flux Density (PPFD) of the leaf surface was controlled at 1000. Mu. Mol m -2 s -1 。
Through systematic investigation of the net photosynthetic rate (Pn) of the transgenic plant in the seedling stage and the heading stage, the positive plant (mut) has lower net photosynthetic rate than the wild plant (WT), and the Lhcb3 gene can affect the chlorophyll content and the photoprotection capacity and the net photosynthetic rate of rice after the expression is down-regulated.
Test example 4 detection of expression level of photosynthesis-related Gene
RNA extraction is carried out, the tissue is ground into powder of about 0.05-0.1g by liquid nitrogen, 1mL of Trans Zol is used, 0.2mL of chloroform is added, the shaking is violent for 15s, and the standing is carried out for 5min at room temperature. Centrifuge at 10,000rpm at 4℃for 10min. At this point the sample was split into three layers. Transfer 600 μl of the supernatant into a new centrifuge tube, add 600 μl isopropanol, mix well, and stand at room temperature for 10min. Centrifugation at 12,000rpm at 4℃for 15min resulted in the formation of a white gummy precipitate on the tube side and bottom. The supernatant fluid is discarded, and the mixture is filtered,1mL of 75% ethanol (DEPC treated water) was added, vortexed vigorously, and centrifuged at 10,000rpm at 4℃for 5min (twice). The supernatant was discarded, centrifuged at 10,000rpm for 1min, the residual ethanol which was not poured off cleanly was sucked off with a gun head, and the pellet was dried (about 10 min) on a sample super clean bench. Adding 50 μl of RNA dissolving solution, and preserving at-70deg.C in 55-60deg.C water bath for 10min for long-term use. After the completion of RNA extraction, reverse transcription was performed using HyperScript III RT SuperMix reverse transcription reagent produced by Shanghai New shellfish Biotechnology, according to the instructions. The expression level of the cDNA after reverse transcription was measured, and amplified using Rogowski fluorescent quantitation reagent FastStart Universal SYBR Green Master (Rox) according to the instructions. According to 2 -ΔΔct The calculation of the relative expression level of the gene was performed.
FIG. 1 is an Lhcb3 gene RNAiT 0 Compared with the positive plant (mut) and the Wild Type (WT), the SPAD value, chlorophyll a, chlorophyll b, total chlorophyll content, fv/Fm and NPQ value of the transgenic plant are extremely obviously reduced; the chlorophyll a/chlorophyll b ratio of the plant is extremely obviously increased; the Lhcb3 gene expression level is well co-separated from SPAD value, chlorophyll a, chlorophyll b, total chlorophyll content, fv/Fm, NPQ value and chlorophyll a/chlorophyll b ratio.
FIG. 2 is an Lhcb3 gene RNAiT 1 SPAD values for two families of transgenic plants in seedling stage and heading stage. The SPAD values of positive plants (mut) were significantly reduced compared to wild-type plants (WT) at both times.
FIG. 3 is an Lhcb3 gene RNAiT 1 Chlorophyll content of the transgenic plants in the seedling stage and the heading stage of the two families; compared with a wild plant (WT), the positive plant (mut) has extremely remarkable reduction of chlorophyll a, chlorophyll b and total chlorophyll content; the chlorophyll a/chlorophyll b ratio of the plant is extremely obviously increased.
FIG. 4 is an Lhcb3 gene RNAiT 1 Fv/Fm values of transgenic plants; the Fv/Fm values of the positive plants (mut) were reduced compared to those of the wild-type plants WT).
FIG. 5 is an Lhcb3 gene RNAiT 1 NPQ value of transgenic plants; the positive plants (mut) compared to the wild type plants WT,the NPQ value thereof is reduced.
FIG. 6 is an Lhcb3 gene RNAiT 1 Net photosynthetic rate of transgenic plants; the maximum net photosynthetic rate of positive plants (mut) was reduced compared to wild-type plants (WT).
FIG. 7 is She Setu of an Lhcb3 gene RNAi transgenic plant; the positive plants (mut) were lighter in colour than the wild type plants (WT).
FIG. 8 is a leaf transmission electron micrograph of an Lhcb3 gene RNAi transgenic plant; mutant chloroplasts are fewer than wild type chloroplasts and are unevenly distributed, and mutant thylakoids are sparsely arranged.
The expression quantity of the transgenic plants is detected to find that: the expression level of PSII-large antenna light-harvesting protein complex genes (Lhcb1.1, lhcb1.2, lhcb1.3, lhcb2 and Lhcb 3) is greatly reduced in the seedling stage and the heading stage; the expression level of PSII-smaller antenna light-harvesting protein complex genes (Lhcb 4, lhcb5, lhcb 6) is slightly reduced in both seedling stage and heading stage; the expression amounts of mutant and wild-type PSI light-harvesting protein complex genes (Lhca 1, lhca3, lhca4 and Lhca 6) are not significantly different; it is exciting that the gene PsbS1 involved in photoprotection is reduced in the heading stage, and the expression level of the gene directly influences the change of NPQ value, and the rice Lhcb3 gene influences the photoprotection capacity by reducing the expression level of PsbS 1.
The primers according to the present invention are shown in Table 1 below
Primer sequences used in Table 1
Sequence listing
<110> Xinyang teaching and learning school
<120> Rice light harvesting pigment chlorophyll a/b binding protein gene Lhcb3 and application thereof in rice photoprotection
<130> 1025
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<170> PatentIn version 3.5
<210> 1
<211> 1346
<212> DNA
<213> Oryza saliva subsp keng
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agcacctccc cttctagagt agtgttagtc ttagcgaatc cagcagcaga agcagcagca 60
gcagctcagc cgccgccgat ggcgtccacg atcatggccc caacctcccg cgtcctcgcc 120
gccaagaccc ccttcctcgg ccatccccgc ccctccaatg cccccgtccg cgacatcgcc 180
gccgccgcca ccggccgcat caccatggta cgtacacact tactctacta ttttccgttc 240
aagaatataa caacctaata tcatcggatg ttcgagaata catcatagtt gttgttatat 300
ttttggacgg aaagagtgca gagtagctaa gtagctattt tttggttgtg ggggtgcaga 360
gcaaggagct gtggtacggg ccggataggg tgaagtacct ggggccattc tcggcgcaga 420
cgccgtcgta cctgaggggc gagttcccgg gcgactacgg gtgggacacg gcggggctct 480
ccgcggaccc ggaggcgttc gcgaggaaca gggcgctgga ggtgatccac ggccggtggg 540
cgatgctcgg cgcgctgggc tgcatcacgc cggaggtgct cgagaagtgg gtgcgcgtcg 600
acttcaagga gcccgtgtgg ttcaaggccg gcgcccagat cttctccgac ggcggcctcg 660
actacctcgg caaccccaac ctggtgcacg cccagagcat cctcgccgtg ctgggattcc 720
aggtcgtcct catgggcctc gtcgagggct accgcatcaa cggcctcccc ggcgtcggcg 780
acggcaacga cctctacccc ggcggccagt acttcgaccc gctcggcctc gccgacgacc 840
ccgtcacctt cgccgagctc aaggtcaagg agatcaagaa tggccgcctc gccatgttct 900
ccatgttcgg cttcttcgtc caggccatcg tcaccggcaa gggccccttg gagaacctgc 960
tcgaccacct cgccgacccc gtcgccaaca acgcctgggt ctacgccacc aagttcacgc 1020
cgggctcgtg aatggcccag ccgccattgt agactactct ctctctccat cgctcgatgt 1080
accaatgctt gcacagattg tacgatgctg tgatgaatct gatatgatcc atccagaatt 1140
cagaaactga aaaaagttgg ggtttgcaac ttatgtgtca gctgctgctt taagcttagt 1200
taactctttt gatgttttaa tttgtacatt tgtgtacttt gtaatgatcc atacgagacc 1260
tagtgtttga ttattgctaa tttgctgtgg cattttcttg catcaagtac tggatacaga 1320
gttcatcatt cagatttttg aagata 1346
Claims (3)
1. Rice light harvesting pigment chlorophyll a/b binding protein geneLhcb3The application in the rice photoprotection is characterized in that: the rice light-harvesting pigment chlorophyll a/b binding protein geneLhcb3The nucleotide sequence is shown as SEQ ID NO. 1, and the rice light-harvesting pigment chlorophyll a/b binding protein geneLhcb3The NPQ value is regulated and controlled by down regulating the expression quantity of a gene PsbS1 involved in photoprotection, so that the photosynthetic yield of the rice is affected.
2. The use according to claim 1, characterized in that: RNAi interference technology is applied to rice light harvesting pigment chlorophyll a/b binding protein geneLhcb3Interference is performed to obtainLhcb3Transgenic plants with suppressed gene function.
3. The use according to claim 1, characterized in that: the sequence SEQ ID NO. 1 is cloned from flower 11 in rice varietyLhcb3DNA sequence of the gene.
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