CN114032230B - 一种细菌甘油脱水酶突变体Gt9及其表达菌株的构建和应用 - Google Patents
一种细菌甘油脱水酶突变体Gt9及其表达菌株的构建和应用 Download PDFInfo
- Publication number
- CN114032230B CN114032230B CN202111633183.7A CN202111633183A CN114032230B CN 114032230 B CN114032230 B CN 114032230B CN 202111633183 A CN202111633183 A CN 202111633183A CN 114032230 B CN114032230 B CN 114032230B
- Authority
- CN
- China
- Prior art keywords
- glycerol dehydratase
- mutant
- solution
- enzyme
- glycerol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 108010025885 Glycerol dehydratase Proteins 0.000 title claims abstract description 61
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 19
- 238000010276 construction Methods 0.000 title claims abstract description 15
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 48
- 102000004190 Enzymes Human genes 0.000 claims abstract description 45
- 108090000790 Enzymes Proteins 0.000 claims abstract description 45
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 23
- 241000186604 Lactobacillus reuteri Species 0.000 claims abstract description 22
- 229940001882 lactobacillus reuteri Drugs 0.000 claims abstract description 17
- 230000035772 mutation Effects 0.000 claims abstract description 8
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract description 6
- 239000000243 solution Substances 0.000 claims description 37
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 claims description 17
- 239000000872 buffer Substances 0.000 claims description 14
- 238000000855 fermentation Methods 0.000 claims description 14
- 230000004151 fermentation Effects 0.000 claims description 14
- 239000000047 product Substances 0.000 claims description 13
- 238000000502 dialysis Methods 0.000 claims description 12
- 230000000694 effects Effects 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 11
- 239000007853 buffer solution Substances 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 238000003259 recombinant expression Methods 0.000 claims description 9
- 239000012634 fragment Substances 0.000 claims description 8
- 238000011084 recovery Methods 0.000 claims description 8
- 230000003321 amplification Effects 0.000 claims description 7
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 7
- 239000013612 plasmid Substances 0.000 claims description 7
- 239000002244 precipitate Substances 0.000 claims description 7
- PHOLIFLKGONSGY-CSKARUKUSA-N (e)-(3-methyl-1,3-benzothiazol-2-ylidene)hydrazine Chemical compound C1=CC=C2S\C(=N\N)N(C)C2=C1 PHOLIFLKGONSGY-CSKARUKUSA-N 0.000 claims description 6
- 241000588724 Escherichia coli Species 0.000 claims description 6
- 210000004027 cell Anatomy 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000002028 Biomass Substances 0.000 claims description 5
- 229910003251 Na K Inorganic materials 0.000 claims description 5
- 239000003480 eluent Substances 0.000 claims description 5
- 239000011534 wash buffer Substances 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 239000000411 inducer Substances 0.000 claims description 4
- 230000006698 induction Effects 0.000 claims description 4
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 claims description 4
- 238000000746 purification Methods 0.000 claims description 4
- 102000003960 Ligases Human genes 0.000 claims description 3
- 108090000364 Ligases Proteins 0.000 claims description 3
- 230000009471 action Effects 0.000 claims description 3
- 210000002421 cell wall Anatomy 0.000 claims description 3
- 238000001952 enzyme assay Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 238000011068 loading method Methods 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims description 3
- 229920002401 polyacrylamide Polymers 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 108091008146 restriction endonucleases Proteins 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- 230000009466 transformation Effects 0.000 claims description 3
- 239000013598 vector Substances 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 claims description 2
- 238000000108 ultra-filtration Methods 0.000 claims description 2
- 239000002299 complementary DNA Substances 0.000 claims 1
- 238000011010 flushing procedure Methods 0.000 claims 1
- 238000002156 mixing Methods 0.000 claims 1
- 238000007747 plating Methods 0.000 claims 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract description 74
- 239000000758 substrate Substances 0.000 abstract description 8
- 230000005764 inhibitory process Effects 0.000 abstract description 6
- 238000011160 research Methods 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000013537 high throughput screening Methods 0.000 abstract description 3
- 108010062877 Bacteriocins Proteins 0.000 abstract description 2
- 230000002906 microbiologic effect Effects 0.000 abstract 1
- 235000011187 glycerol Nutrition 0.000 description 24
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 14
- 238000006243 chemical reaction Methods 0.000 description 12
- 239000000499 gel Substances 0.000 description 12
- 102220485915 Putative uncharacterized protein DHRS4-AS1_C20S_mutation Human genes 0.000 description 8
- 238000001514 detection method Methods 0.000 description 8
- AKXKFZDCRYJKTF-UHFFFAOYSA-N 3-Hydroxypropionaldehyde Chemical compound OCCC=O AKXKFZDCRYJKTF-UHFFFAOYSA-N 0.000 description 7
- 239000011780 sodium chloride Substances 0.000 description 7
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 6
- 238000001976 enzyme digestion Methods 0.000 description 6
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 5
- 229960000723 ampicillin Drugs 0.000 description 5
- 238000000246 agarose gel electrophoresis Methods 0.000 description 4
- 230000001351 cycling effect Effects 0.000 description 4
- 150000002460 imidazoles Chemical class 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 238000012257 pre-denaturation Methods 0.000 description 4
- 206010010144 Completed suicide Diseases 0.000 description 2
- 229930003779 Vitamin B12 Natural products 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 2
- 238000007872 degassing Methods 0.000 description 2
- 239000012149 elution buffer Substances 0.000 description 2
- 239000013613 expression plasmid Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000008057 potassium phosphate buffer Substances 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 238000001742 protein purification Methods 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000000967 suction filtration Methods 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- 239000011715 vitamin B12 Substances 0.000 description 2
- 235000019163 vitamin B12 Nutrition 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000005536 corrosion prevention Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 150000002314 glycerols Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000001508 potassium citrate Substances 0.000 description 1
- 229960002635 potassium citrate Drugs 0.000 description 1
- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 description 1
- 235000011082 potassium citrates Nutrition 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/01—Hydro-lyases (4.2.1)
- C12Y402/0103—Glycerol dehydratase (4.2.1.30)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/101—Plasmid DNA for bacteria
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111633183.7A CN114032230B (zh) | 2021-12-29 | 2021-12-29 | 一种细菌甘油脱水酶突变体Gt9及其表达菌株的构建和应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111633183.7A CN114032230B (zh) | 2021-12-29 | 2021-12-29 | 一种细菌甘油脱水酶突变体Gt9及其表达菌株的构建和应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN114032230A CN114032230A (zh) | 2022-02-11 |
CN114032230B true CN114032230B (zh) | 2023-07-04 |
Family
ID=80147257
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202111633183.7A Active CN114032230B (zh) | 2021-12-29 | 2021-12-29 | 一种细菌甘油脱水酶突变体Gt9及其表达菌株的构建和应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN114032230B (zh) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN117511762A (zh) * | 2023-09-14 | 2024-02-06 | 石河子大学 | 一株产reuterin的耐盐罗伊氏乳杆菌及其应用 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112877304A (zh) * | 2021-03-15 | 2021-06-01 | 合肥师范学院 | 一种细菌漆酶突变体LacAt及其表达菌株的构建和应用 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104053766B (zh) * | 2011-11-30 | 2016-05-04 | 热尔韦·达诺尼公司 | 产生罗伊氏菌素的短乳杆菌 |
WO2013137277A1 (ja) * | 2012-03-14 | 2013-09-19 | 株式会社日本触媒 | 3-ヒドロキシプロピオン酸の製造方法、遺伝子組換え微生物、並びに前記方法を利用したアクリル酸、吸水性樹脂、アクリル酸エステル、およびアクリル酸エステル樹脂の製造方法 |
CN105567622B (zh) * | 2016-03-02 | 2019-10-29 | 浙江工业大学 | 一种重组大肠杆菌及合成3-羟基丙酸中的应用 |
-
2021
- 2021-12-29 CN CN202111633183.7A patent/CN114032230B/zh active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112877304A (zh) * | 2021-03-15 | 2021-06-01 | 合肥师范学院 | 一种细菌漆酶突变体LacAt及其表达菌株的构建和应用 |
Also Published As
Publication number | Publication date |
---|---|
CN114032230A (zh) | 2022-02-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US10829755B2 (en) | Genetically engineered arginine deiminase modified by site-directed mutagenesis | |
CN111876404B (zh) | 一种醛缩酶突变体及其编码基因和应用 | |
CN112725319B (zh) | polyG底物特异性的褐藻胶裂解酶FaAly7及其应用 | |
CN105543193A (zh) | 一种n-酰基高丝氨酸内酯酶及其编码基因和重组菌 | |
CN112029752B (zh) | 一种石莼多糖裂解酶及其编码基因与应用 | |
CN114032230B (zh) | 一种细菌甘油脱水酶突变体Gt9及其表达菌株的构建和应用 | |
CN115960875A (zh) | 一种热稳定性提高的褐藻胶裂解酶的突变体酶 | |
CN107603994B (zh) | 一种κ-卡拉胶酶及其基因和应用 | |
CN112143743B (zh) | 一种乙醛脱氢酶基因、大肠杆菌工程菌、表达及应用 | |
CN111334488B (zh) | 昆布多糖酶ouc-l1及其编码基因与应用 | |
CN111057695B (zh) | 一种腈水解酶及其制备方法和应用 | |
JP4561009B2 (ja) | D−ヒダントインハイドロラーゼをコードするdna、n−カルバミル−d−アミノ酸ハイドロラーゼをコードするdna、該遺伝子を含む組み換えdna、該組み換えdnaにより形質転換された細胞、該形質転換細胞を用いるタンパク質の製造方法、および、d−アミノ酸の製造方法 | |
CN114457062B (zh) | 用于制备褐藻寡糖的褐藻胶裂解酶及其用途 | |
CN115960879A (zh) | 一种d-阿洛酮糖3-差向异构酶突变体文库的高通量筛选方法及获得的突变体 | |
CN114181927B (zh) | 一种肝素酶i | |
CN111471667B (zh) | 壳聚糖酶Csn-PT及其应用 | |
CN112410353B (zh) | 一种fkbS基因、含其的基因工程菌及其制备方法和用途 | |
JP4774496B2 (ja) | α−マンノシダーゼ | |
CN111607548B (zh) | 一种产甘露聚糖的重组大肠杆菌及其应用 | |
CN117737024B (zh) | 一种糖基转移酶突变体及酶法制备急性缺血性脑卒中候选药物shpl-49的方法 | |
CN115851684B (zh) | 一种腈水解酶及其在蛋氨酸合成中的应用 | |
CN117586991B (zh) | β-N-乙酰氨基己糖苷酶FlaNag2353及其应用 | |
CN114958801B (zh) | 产物耐受型腈水解酶突变体及其应用 | |
US10900028B2 (en) | Heparinase-producing Pseudomonas stutzeri strain and heparinase derived therefrom | |
JP3765758B2 (ja) | 新規なd−アミノアシラーゼをコードするdnaおよびそれを用いたd−アミノ酸の製造方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20231226 Address after: 236400 Road South, 10 meters east of the intersection of Tengfei Road and Fuquan Road, Xingtang Street Office, Linquan County, Fuyang City, Anhui Province Patentee after: Linquan Junxuan Biotechnology Co.,Ltd. Address before: 230001 No.40, Nongke South Road, Luyang District, Hefei City, Anhui Province Patentee before: AGRICULTURAL ENGINEERING INSTITUTE, ANHUI ACADEMY OF AGRICULTURAL SCIENCES |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240426 Address after: 236400 the intersection of Fuquan road and Tengfei Road, Xingtang street, Linquan County, Fuyang City, Anhui Province Patentee after: Linquan Guoneng Natural Gas Co.,Ltd. Country or region after: China Address before: 236400 Road South, 10 meters east of the intersection of Tengfei Road and Fuquan Road, Xingtang Street Office, Linquan County, Fuyang City, Anhui Province Patentee before: Linquan Junxuan Biotechnology Co.,Ltd. Country or region before: China |
|
TR01 | Transfer of patent right |