CN114027424B - Special puffed feed for circulating water culture of striped bass juvenile fish as well as preparation method and application of special puffed feed - Google Patents
Special puffed feed for circulating water culture of striped bass juvenile fish as well as preparation method and application of special puffed feed Download PDFInfo
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Abstract
The invention discloses a special puffed feed for circulating water culture of striped bass, which comprises, by mass, 54% of protein, 16% of fat and 3.375% of egg fat: 1. the invention also discloses a preparation method and application thereof. The special puffed feed for the circulating water culture of the striped bass comprises 54% of protein and 16% of fat, and the egg fat ratio is 3.375:1, the young striped bass fed with the striped bass feed has the best growth performance under the condition of factory circulating water cultivation.
Description
Technical Field
The invention relates to the technical field of fish puffed feed, in particular to a puffed feed special for circulating water culture of young striped bass, a preparation method and application thereof.
Background
Striped bass, academic name Centropristis striata, also known as America black spot, is taxonomically assigned to Perciformes (Perciformes) > Perciformes (Porcoidei) > bass (SERRANIDAE) > bass genus (Centropristis) > striped bass (Centropristis striata). Striped bass is a rare marine fish which is mainly distributed on the east coast of the United states and Mexico, namely the Atlantic coast, and has a plurality of excellent qualities of high growth speed, strong adaptability, high meat yield, rich nutrition and the like. Researchers analyze and evaluate the nutrition components, and prove that the striped bass is an excellent breeding variety with higher nutrition value and economic value and wide market prospect.
Striped bass belongs to fishes with wide temperature, wide salt and warm temperature, the survival temperature is 5-31 ℃, and the optimal growth temperature is 17-25 ℃; the survival salinity is 10-35, and the optimal growth salinity is 23-33. The striped bass has lower oxygen consumption rate and can resist 3.0mg/L hypoxia condition, so that the striped bass can be densely cultivated. Striped bass is carnivorous fish, is relatively fierce, is initially cultivated, is relatively timidic due to the change of environment, cannot eat immediately, and needs to be acclimatized for a certain time. Under the condition of artificial culture, the growth speed of the plant is many times faster than that of the plant in natural environment; the raised black stone spots can be quickly suitable for ingestion of dry granular feed; the cultivation effect in a closed or open cultivation system is good.
Striped bass in 2002 is introduced into Shandong province in China, and domesticated and artificially bred successfully in 2006. The cultivation is more in Fujian areas of China, the quantity of other areas is small, but the market is gradually faded out due to the general sales. In recent years, the cultivation scale is enlarged because of the excellent performance of the cultivation scale in the tattooing industry, which is popular with consumers. With the development of large-scale cultivation of striped bass in areas such as Fujian, guangdong, shandong and Zhoushan in China, the need of providing high-quality compound feed capable of meeting the growth needs of striped bass is urgent, and the research on striped bass in China is mainly focused on the aspects of resource investigation, ecology, physiology, artificial breeding and cultivation technology and the like, and few research reports on the nutrition needs of striped bass are provided, so that a series of researches on the protein level, fat level and egg fat ratio in the compound feed are carried out to obtain the optimal feed egg fat ratio required by the growth of striped bass.
Protein and fat are important substance bases required for normal growth and development of fish, wherein the protein is the most critical nutrient for determining the growth of the fish, and has obvious influence on the growth and development of the fish. In livestock production, high-precision feed and high-protein feed are often selected for intensive fattening, and because of the high protein nutrition level, the feed conversion rate is superior to that of low-protein feed, and the protein requirements of aquatic animals are far higher than those of livestock and poultry animals. When the protein content in the feed is insufficient and the nutrition requirement of normal growth and development of the feed cannot be met, the growth speed of fish and the utilization efficiency of the feed are reduced, even the disease resistance of organisms of the fish is reduced, and the adaptation tolerance and the anti-stress capability to adverse environments are reduced; when the protein content in the feed is too high, the aquaculture cost is increased, excessive ammonia nitrogen excreta can be generated by fish due to excessive protein intake, the pollution of the aquaculture water body can be aggravated to a certain extent, the occurrence of diseases is increased, and meanwhile, the protein resource waste can be caused.
Non-protein energy sources have proven useful in reducing protein consumption as energy and increasing protein utilization for growth, a process known as "protein conservation". However, the use of non-protein energy sources in aquaculture feeds must be evaluated because the addition of too much non-protein energy sources in aquaculture feeds has several disadvantages: (1) reducing feed intake; (2) the fat content of the fish body is too high; (3) inhibit the use of other nutrients. The content and the proportion of protein and fat in the feed are not only related to the economic benefit of fish culture, but also relate to the ecological environment problem. Thus, in addition to meeting the nutritional requirements of aquatic feed formulations, optimal feed egg/fat ratios should be considered.
Disclosure of Invention
The invention aims to provide a special puffed feed for circulating water culture of striped bass and a preparation method and application thereof, and aims to solve the defects in the prior art.
The invention adopts the following technical scheme:
The invention provides a special puffed feed for circulating water culture of striped saw bass, which comprises, by mass, 54% of protein, 16% of fat and 3.375% of egg fat: 1.
Further, the special puffed feed for circulating water culture comprises Peruvian fish meal, defatted antarctic krill meal, soybean meal, wheat starch, wheat gluten, soybean milk powder, fish oil, soybean oil, premix, choline chloride, L-lysine and DL-methionine, wherein the premix consists of vitamin premix and mineral premix.
The invention provides a preparation method of special puffed feed for circulating water aquaculture of striped bass, which comprises the following steps:
step 1, respectively crushing Peruvian fish meal, defatted antarctic krill powder and soybean meal, and sieving with a 100-mesh sieve;
Step 2, weighing Peruvian fish meal, defatted antarctic krill powder, bean pulp, wheat starch, wheat gluten and soybean milk powder according to the formula amount, fully and uniformly mixing in a double-shaft mixer, weighing premix, choline chloride, L-lysine and DL-methionine according to the formula amount, uniformly mixing, adding into the double-shaft mixer, fully and uniformly mixing, and slowly adding 45% -50% of water according to the total mass of Peruvian fish meal, defatted antarctic krill powder, bean pulp, wheat starch, wheat gluten, soybean milk powder, premix, choline chloride, L-lysine and DL-methionine, further uniformly mixing to obtain wet materials, and standing for 24 hours at normal temperature to balance the moisture of the wet materials;
Step 3, granulating by using a double-screw bulking machine, wherein the aperture of a discharging mould is 4.0mm, and rapidly drying and shaping the feed by using a dryer and controlling the moisture to be 7% -9%;
step 4, using a vacuum spraying machine to spray grease, wherein the grease is fish oil and soybean oil;
and 5, sieving and removing broken particles to obtain the special puffed feed for circulating water aquaculture of the young striped bass.
The third aspect of the invention provides the application of the special puffed feed for circulating aquaculture in feeding striped bass juvenile fish.
Further, the initial average weight of the young bass is 42.2+/-0.33 g.
The invention has the beneficial effects that:
According to the invention, through a compound feed feeding experiment for 70 days, feeding, growth, feed utilization, total fish components and nutrient storage rate are taken as comprehensive evaluation indexes, and the optimal protein demand of striped saw bass (with initial average weight of 42.2+/-0.33 g) is 54% and the optimal fat demand is 16% is obtained through single-factor and double-factor variance analysis.
The growth of the striped bass juvenile fish is obviously affected by the egg fat ratio of different feeds, the striped bass juvenile fish fed with the D5 (54/16) feed has the best growth performance under the condition of industrial circulating water culture, and the optimal egg fat ratio of the special puffed feed for the striped bass juvenile fish circulating water culture is 3.375:1.
Detailed Description
The invention is further explained below with reference to examples. The following examples are only illustrative of the present invention and are not intended to limit the scope of the invention.
1 Experimental materials and methods
1.1 Main raw materials and Experimental feeds
The experiment uses a 2x 4 two factor design, 8 experimental groups were set up, comprising 2 fat levels (12%, 16%) and 4 protein levels (54%, 50%, 46% and 42%), D1 (54/12), D2 (50/12), D3 (46/12), D4 (42/12), D5 (54/16), D6 (50/16), D7 (46/16) and D8 (42/16), respectively. The formulation and chemical composition of the eight feeds are shown in Table 1. The premix in table 1 is a multidimensional multi-mineral premix, which consists of vitamin premix and mineral premix, and the formula is as follows:
Vitamin premix (mg kg -1 feed): vitamin a,1.95; vitamins B1, 20; vitamins B2, 10; vitamin B6, 30; nicotinamide, 250; vitamin C,5; calcium pantothenate, 50; folic acid, 20; vitamin E,1200; vitamin K,0.8; vitamin D,0.05; inositol, 650; defatted rice bran, 150.
Mineral premix (mg kg -1 feed )：CuSO4·5H2O,10;FeSO4·7H2O,300;ZnSO4·H2O,200;MnSO4·H2O,100;KI(10％),80;Na2SeO3(10％Se),67;CoCl2·6H2O(10％Co),5;NaCl,100; zeolite powder 638).
Pulverizing Peruvian fish powder, defatted Euphausia superba powder and soybean meal respectively, and sieving with 100 mesh sieve. Weighing Peruvian fish meal, defatted antarctic krill powder, bean pulp, wheat starch, wheat gluten and soybean milk powder according to the formula amount, fully and uniformly mixing in a double-shaft stirrer, weighing premix, choline chloride, L-lysine and DL-methionine according to the formula amount, uniformly mixing, adding into the double-shaft stirrer, fully and uniformly mixing, and slowly adding 45% -50% of water according to the total mass of Peruvian fish meal, defatted antarctic krill powder, bean pulp, wheat starch, wheat gluten, soybean milk powder, premix, choline chloride, L-lysine and DL-methionine, and further uniformly mixing to obtain wet materials. The mixture was left at room temperature for 24 hours to equilibrate the moisture of the wet material. The feed processing was carried out using a twin screw bulking machine (SYSLG-IV, saibano technology Co., ltd., jinan) with a discharge die aperture of 4.0mm. The fodder is quickly dried and shaped by a dryer, and the moisture is controlled between 7% and 9%. Grease (fish oil and soybean oil) spraying was performed using a ZJB-40 vacuum coater. After sieving and removing the broken particles, 3 x 1000 complete feeds are randomly selected from each feed and weighed, so that the average particle weight of each feed is obtained.
1.2 Determination of physical Properties of Experimental feeds
Physical performance metrics of the experimental feed included Length (L), particle size (Diameter, D), feed durability index (Pellet durability index, PDI), expansion rate (E), and hardness (Hardness of pellets, HP).
50 Complete feeds are randomly selected from each feed (not sprayed), and the length L and the particle size D of the complete feeds are measured and recorded by a digital vernier caliper.
Pellet feed durability index PDI is one of the most important indicators reflecting pellet feed quality and is used to measure the relative ability of a pellet feed product to resist breakage of the feed pellet during transportation and handling. The experiment was performed using a ST-136 double-box feed pulverization tester (Cheng Tai instruments, jinan) to determine PDI of the self-made experimental feed. 500g of granular feed (marked as M) without sprayed grease is placed in a rotary box of a pulverizer, the rotation speed of the instrument is 50r/min, the operation is continued for 10 minutes, a sample is taken out after the instrument stops working, a standard sieve with 20 meshes and the aperture of 0.9mm is used for sieving the sample by hand for about 2 minutes, and the weight of the oversize is marked as M. Repeating the steps once, and taking the average value of the two measurement results to obtain the final pulverization rate of the feed.
PDI calculation formula:
Wherein:
m-weight of sample (g) on sieve after sieving;
M-initial sample weight (g).
The calculation mode of the feed expansion rate E is as follows:
Wherein:
D 1 —particle size (mm) of pellet feed;
D 2 -the aperture (mm) of the discharge orifice.
The hardness of the feed was measured by using an ST-120B hardness tester (Cheng Tai instruments, inc., jinan). 50 feed pellets were randomly selected from each of the non-fat-sprayed feeds, placed in a hardness tester to measure the readings and record, and averaged. The physical properties of all 8 homemade test feeds are detailed in table 2.
1.3 Experimental fish and feeding management
The striped bass juvenile fish in the experiment is purchased at Zhu Gujian th month of 2019 in the aquatic product culture institute of Zhoushan city, temporarily cultured in a laboratory of the aquatic product animal nutrition and feed resource research institute of Zhejiang university until the experimental specification, and then transferred into an indoor seawater circulation culture system for formal experiment. The striped bass juvenile fish were starved for 24 hours prior to the experiment and then anesthetized with MS-222 anesthetic. 600 striped bass juvenile fish (initial average weight of about 42.2 + -0.33 g) which are healthy, strong and harmless in body and have basically consistent body length and body weight are selected, weighed and randomly placed into 24 cylindrical glass fiber cylinders with the volume of 500L, and 25 feet per cylinder. Three parallel cylinders for each feed. In the cultivation process, the light is irradiated for 15 hours every day, oxygen is continuously supplied through the gas stone for 24 hours, the water changing amount of each cylinder is 4-5L/min, the water salinity for cultivation is 24-26, the pH value is 7.0-7.5, the water temperature is 25-28 ℃, the dissolved oxygen is more than 5.5mg/L, the ammonia nitrogen content is less than or equal to 0.5mg/L, and the nitrite content is less than or equal to 0.25mg/L.
After the growth experiment is started, 3 times a day (8:00, 14:00, 20:00) are fed for about 1 hour each time, so that the apparent satiety of each experimental fish is ensured, and the fish is not fed any more. The daily feeding amount of each jar is increased by 10% based on the average net feeding amount of three days before the jar, and the residual bait amount is calculated by multiplying the residual bait amount generated after each feeding time by the average weight of the corresponding feed particles of each jar. The feeding experiments were performed for a total of 70 days.
1.4 Sample collection and analysis
1.4.1 Sample collection
10 Striped bass juvenile fish which are basically consistent with the body weight of the experimental fish and are healthy and active are selected as initial whole fish samples before the growth experiment starts, and the striped bass juvenile fish are anesthetized and killed by using excessive MS-222, weighed and stored in a refrigerator at the temperature of minus 20 ℃. Feeding is stopped the day before final sampling, and each experimental fish is anesthetized by MS-222 (1.0 mg/L) anesthetic, drained, and the total weight is weighed after excessive water on the surface of the fish body is wiped off. Then 10 individuals with uniform size are selected from the above materials, and the body length and the body weight are measured and then are placed in a refrigerator at the temperature of minus 20 ℃ to be used as whole fish samples.
1.4.2 Sample analysis
The initial and final whole fish samples of each jar were separately stirred into surimi using an SXC-12 meat grinder (hengyue food machinery, shanghai), each sample was mixed well and sampled to determine the fresh sample moisture of the whole fish. The whole minced fillet samples were autoclaved at 120℃for 30 minutes using a DGL-758 vertical steam sterilizer (Denguan medical instruments Co., ltd., jiangsu) and homogenized in a tissue homogenizer, and then dried in an oven at 80℃until the moisture content was less than or equal to 9%. And (3) crushing the dried fish by using a grinder, sieving the crushed fish with a 30-target standard sieve to obtain whole fish dry powder, and filling the whole fish dry powder into a sample bottle to be tested.
The water content is measured by a constant weight method of drying at 105 ℃, and the water is dried for 36 to 48 hours by using a DHG-9140A oven (Shanghai, inc. of Jinghai laboratory equipment); the ash was measured by burning in a SX2-4-10A muffle furnace (Shangdaozhi Ind) at 550℃for 30 hours; the crude protein was measured by Kjeldahl method using Opsis KD's full-automatic Kjeldahl apparatus (OPSIS, switzerland); crude fat was measured by Soxhlet extraction using Opsis SX-360 Soxhlet extractor OPSIS, switzerland). The total energy of the sample was measured by the oxygen bomb method using a Parr-1271 model fully automatic oxygen bomb calorimeter (Parr, USA).
1.5 Data calculation and statistical analysis
The formulas for food intake (FEED INTAKE, FI), fish weight gain (WEIGHT GAIN, WG), weight gain rate (WEIGHT GAIN RATE, WGR), feed coefficient (Feed conversion ratio, FCR), protein storage rate (Protein retention efficiency, PRE), energy storage rate (Energy retention efficiency, ERE) are as follows:
FI (g dry matter/tail) =total feed intake/mantissa
WG (g/tail) =final average weight-initial average weight
WGR (%) =weight gain/initial average weight x 100%
FCR = food intake/weight gain
PRE (%) = (final average weight x final whole fish crude protein content-initial average weight x initial whole fish crude protein content)/(feed dry basis crude protein content x food intake) x 100%
ERE (%) = (end average weight x end total fish energy value-initial average weight x initial total fish energy value)/(feed dry basis energy value x food intake) x 100%
The data of four different protein content feed groups at the same fat level are statistically analyzed by using One-way ANOVA method, and if the difference (P < 0.05) is significant, regression analysis is further performed to estimate a linear or quadratic curve. Two factors were analyzed statistically for different fat levels and different protein levels using Two-way ANOVA. All this was done using SPSS 20.0 software.
2 Experimental results and analysis
2.1 Effect of egg lipid ratio of different feeds on feeding, growth and feed utilization of striped bass
The different feed egg lipid ratios have significant effects (P < 0.05) on the feed intake, weight gain rate and feed coefficient of the young striped bass, and the results are shown in Table 3. Young striped bass fed with feed D1 (54/12) and feed D5 (54/16) have the highest and similar feeding, weight gain and weight gain rates, the highest feeding amount is up to 73.1 g/tail, the weight gain is up to 50.2 g/tail, the weight gain rate is up to 119%, the weight gain rate is significantly higher than other 3 feeds (P < 0.05) with the same fat level, and the feed coefficient is also significantly lower than other groups (P < 0.05) with the same fat level. Double-factor analysis of variance (Table 4) was performed on the ingestion, growth and feed utilization of young striped bass at both fat and protein levels, and it was found that the different fat levels had a significant effect on the ingestion, growth and feed utilization of experimental fish as well (P < 0.05), but the interaction of protein and fat had no significant effect on the various growth indicators of young striped bass (P > 0.05). The feed intake, weight gain, and weight gain rate decrease with decreasing protein content and vice versa with varying fat content. The feed coefficient increases with decreasing protein content or fat content. In conclusion, based on the experimental result, the feed D5 (54/16) has better performance on the aspects of ingestion, growth and feed utilization of experimental juvenile fish.
2.2 Effect of different feed egg lipid ratios on the Whole fish composition of striped bass juvenile fish
As shown in Table 5, the different feed egg lipid ratios had a significant effect on the whole fish crude protein of experimental fish fed with 16% fat level feed only on the components (P < 0.05), but there was no significant difference between the treatment groups on dry matter, crude fat, ash, energy, 12% fat level whole fish crude protein, etc. (P > 0.05). Based on regression analysis, at 16% fat level, the crude protein content of fish body was linear with the protein content in the feed (table 5, crude protein=0.92x+135, r2=0.83, where X represents the protein content in the feed). According to Table 6, the change in fat content has a significant effect (P < 0.05) on the dry matter, crude fat, and total time of the whole striped bass juvenile fish. The change of the protein content in the feed only has a significant effect on the crude protein of the whole fish of the experimental fish (P < 0.05). From Table 7 and Table 8, the egg fat ratio of different feeds showed no significant difference (P > 0.05) in protein storage rate and energy storage rate of the young striped bass.
2.3 Proper protein and fat content and optimal egg fat ratio in feed for young striped bass
According to Table 3, the treatment groups with different protein contents were compared with each other at the same fat level, namely D1 (54/12), D2 (50/12), D3 (46/12), D4 (42/12) or D5 (54/16), D6 (50/16), D7 (46/16) and D8 (42/16). The feed treatment group with high protein content (54%) has significantly higher ingestion, growth and feed utilization than other treatment groups (P < 0.05), and is more suitable for the growth of young striped bass. Thus, the protein content of 54% in the feed is primarily determined to be the optimal protein content required by the growth of the young striped bass. Comparison between the treatment groups of feeds with different fat contents at the same protein level shows that the ingestion, growth and feed utilization of the high-fat group (16%) are higher than those of the low-fat group (12%), so that the fat content of 16% in the feed is primarily judged to be the fat content required for the optimal growth of young striped-saw fish. In combination with tables 5 and 6, the change of the protein content in the feed only has a significant effect on the crude protein of the whole fish (P is less than 0.05), and the crude protein content of the whole fish of the experimental fish increases with the increase of the protein content in the feed, and no significant difference exists after the protein content in the feed reaches 50%. The change of fat content in the feed has a significant effect on the dry matter of the fish body, the crude fat content and the total energy (P < 0.05), and the dry matter of the whole fish of the experimental fish, the crude fat content and the total energy increase with the increase of the fat content in the feed. As can be seen from tables 7 and 8, the change in protein content or fat content in the feed had no significant effect on the protein storage rate and the energy storage rate of the whole fish (P > 0.05). In conclusion, the optimal protein content in the puffed feed special for the circulating water culture of the striped bass is about 54%, the fat content is about 16%, and the egg fat ratio is 3.375:1.
Conclusion 3
1. The optimal protein demand of young striped bass (initial average weight is about 42.2+/-0.33 g) is 54% and the optimal fat demand is 16% by taking ingestion, growth, feed utilization, total fish components and nutrient storage rate as comprehensive evaluation indexes and performing single-factor and double-factor analysis of variance.
2. The egg fat ratio of different feeds has a remarkable influence on the growth of young striped bass (P < 0.05), young striped bass fed with D5 (54/16) feed has the best growth performance under the condition of industrial circulating water culture, and the optimal egg fat ratio of the special expanded feed for circulating water culture of young striped bass is 54 percent: 16%, 3.375:1.
Table 1 experimental feed formulation for striped bass and young fish and analysis of chemical composition (dry matter basis)
Table 2 physical properties of 8 puffed feeds for young striped bass
TABLE 3 egg fat ratio of different feeds the effects of feeding, growth and feed utilization of striped bass larvae (based on one-way analysis of variance)
Different superscript letters a,b and c indicate significant differences between the different treatment groups (P < 0.05).
1 And integrating the average standard error.
Table 4 effects of different feed egg lipid ratios on feeding, growth and feed utilization of striped bass juvenile fish (based on two-factor analysis of variance)
Different superscript letters a,b and c indicate significant differences between the different treatment groups (P < 0.05).
1 And integrating the average standard error.
TABLE 5 Effect of egg lipid ratios of different feeds on the composition of the complete fish of striped bass (based on one-factor analysis of variance)
Different superscript letters a,b and c indicate significant differences between the different treatment groups (P < 0.05).
1 And integrating the average standard error.
TABLE 6 Effect of egg lipid ratios of different feeds on the composition of the complete fish of striped bass (based on two-factor analysis of variance)
Different superscript letters a,b and c indicate significant differences between the different treatment groups (P < 0.05).
1 And integrating the average standard error.
TABLE 7 Effect of egg lipid ratios of different feeds on protein and energy storage rates of striped bass juvenile fish (based on one-factor analysis of variance)
1 And integrating the average standard error.
TABLE 8 Effect of egg lipid ratios of different feeds on protein and energy storage rates of striped bass juvenile fish (based on two-factor analysis of variance)
Different superscript letters a and b indicate significant differences between the different treatment groups (P < 0.05).
1 And integrating the average standard error.
Claims (4)
1. The special puffed feed for the circulating water aquaculture of the striped bass is characterized by comprising, by mass, 54% of protein, 16% of fat and 3.375 of egg fat: 1, a step of; the special puffed feed for circulating water culture comprises Peruvian fish meal, defatted antarctic krill powder, bean pulp, wheat starch, wheat gluten, soybean milk powder, fish oil, soybean oil, premix, choline chloride and DL-methionine, wherein the premix consists of vitamin premix and mineral premix;
The specific contents of the components calculated by g.kg -1 feed are as follows: peruvian fish meal 250, defatted antarctic krill meal 40, soybean meal 95, wheat starch 117, wheat gluten 75, soybean milk powder 280, fish oil 70, soybean oil 61, premix 10, choline chloride 0.5 and DL-methionine 0.6;
Calculating the vitamin premix according to mg.kg -1 feed: vitamin a,1.95; vitamins B1, 20; vitamins B2, 10; vitamin B6, 30; nicotinamide, 250; vitamin C,5; calcium pantothenate, 50; folic acid, 20; vitamin E,1200; vitamin K,0.8; vitamin D,0.05; inositol, 650; defatted rice bran, 150;
The mineral premix ：CuSO4·5H2O,10;FeSO4·7H2O,300;ZnSO4·H2O,200;MnSO4·H2O,100;KI,80;Na2SeO3,67;CoCl2·6H2O,5;NaCl,100; zeolite powder 638 was calculated as mg.kg -1 feed.
2. The method for preparing the puffed feed special for circulating water culture of striped bass and bass according to claim 1, which is characterized by comprising the following steps:
step 1, respectively crushing Peruvian fish meal, defatted antarctic krill powder and soybean meal, and sieving with a 100-mesh sieve;
Step 2, weighing Peruvian fish meal, defatted antarctic krill powder, bean pulp, wheat starch, wheat gluten and soybean milk powder according to the formula amount, fully and uniformly mixing in a double-shaft mixer, weighing premix, choline chloride and DL-methionine according to the formula amount, uniformly mixing, adding into the double-shaft mixer, fully and uniformly mixing, slowly adding 45% -50% of water according to the total mass of Peruvian fish meal, defatted antarctic krill powder, bean pulp, wheat starch, wheat gluten, soybean milk powder, premix, choline chloride and DL-methionine, further uniformly mixing to obtain wet materials, and standing at normal temperature for 24 hours to balance the moisture of the wet materials;
Step 3, granulating by using a double-screw bulking machine, wherein the aperture of a discharging mould is 4.0mm, and rapidly drying and shaping the feed by using a dryer and controlling the moisture to be 7% -9%;
step 4, using a vacuum spraying machine to spray grease, wherein the grease is fish oil and soybean oil;
and 5, sieving and removing broken particles to obtain the special puffed feed for circulating water aquaculture of the young striped bass.
3. Use of the expanded feed for circulating aquaculture according to claim 1 for feeding young bass with striped saw.
4. The use according to claim 3, wherein the young bass is initially weighed 42.2±0.33g.
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