CN114010560A - Compound Tibetan herba schizonepetae composition with effects of brightening skin and resisting allergy and relieving and preparation method thereof - Google Patents

Compound Tibetan herba schizonepetae composition with effects of brightening skin and resisting allergy and relieving and preparation method thereof Download PDF

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CN114010560A
CN114010560A CN202111366824.7A CN202111366824A CN114010560A CN 114010560 A CN114010560 A CN 114010560A CN 202111366824 A CN202111366824 A CN 202111366824A CN 114010560 A CN114010560 A CN 114010560A
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composition
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CN114010560B (en
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旦增江白
李斌
黄山
吾坚旦增
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Bange Zhuopan Health Industry Co ltd
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18

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Abstract

The invention discloses a compound Tibetan schizonepeta composition with skin brightening, anti-allergy and relieving effects and a preparation method thereof, wherein the compound Tibetan schizonepeta composition is prepared from the following raw materials in parts by weight: 10-50 parts of Tibetan schizonepeta, 10-50 parts of corydalis tuber, 5-30 parts of tribulus fruit and 5-30 parts of myrobalan. The invention also comprises a preparation method and application of the composition. The composition disclosed by the invention has the functions of brightening the skin and resisting allergy and relieving when being used in a skin care product, and has milder skin effect, no irritation and higher safety.

Description

Compound Tibetan herba schizonepetae composition with effects of brightening skin and resisting allergy and relieving and preparation method thereof
Technical Field
The invention relates to the technical field of fine chemical and daily necessities, in particular to a compound Tibetan schizonepeta composition with skin brightening, anti-allergy and relieving effects and a preparation method thereof.
Background
Skin color, which is the color of the skin, is the color of the epidermis of the human skin as reflected by pigmentation of the epidermis of the human skin, such as melanin, protopanarubicin, and phyllanthin. Skin color is distributed differently in different areas and populations. Skin color is related to the content and distribution (granular or dispersed) of melanin in the skin. Melanin is concentrated in and among the cells of the germinal layer of the epidermis. The dermis is generally devoid of melanin, but with pigments, it can penetrate the skin and appear cyan, such as gray-cyan patches of the sacral and buttocks of a newborn. In addition, the color of the skin is also related to the blood in the capillaries, the roughness and the moisturization of the skin.
Most Asian women want to have bright and white skin, but under the conditions that the work and life rhythm of the modern society is accelerated and the pressure is increased, many Asian women have the skin health problems of dull and dull skin and increased skin darkness, which causes troubles to the daily life and work of the Asian women. Thus, the need for skin lightening products is also an urgent requirement for asian female skin care products.
With the frequent use of various skin care and cosmetic products and the increase of external adverse stimuli such as environmental pollution, the proportion of people with sensitive skin is increasing. Sensitive skin is easy to have symptoms of reddening, fever, pruritus, stabbing pain and the like, and skin redness and swelling and rash can occur in severe cases. Sensitive skin also causes the problems of skin thinning, stratum corneum insufficiency, easy desquamation, poor water retention capacity, tight and dry skin and the like. With the increasing quality requirements of skin care and cosmetic products, consumers also want products that are milder, non-irritating, and safer to the skin.
At present, skin care products which can brighten skin color and resist allergy and relieve allergy are still rare. The products with the skin color brightening function mostly adopt vitamin C, tartaric acid, kojic acid, tretinoin and the like, the raw materials are easy to cause the problems of red swelling, fever, pruritus, stabbing pain and the like of sensitive skin, and the products do not brighten the skin color but cause new skin problems. Therefore, consumers want the product to have both effects, and a new solution is provided for solving the skin problem.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides the compound Tibetan schizonepeta composition with the functions of brightening the skin and resisting allergy and relieving and the preparation method thereof.
In order to achieve the purpose, the technical scheme adopted by the invention for solving the technical problems is as follows: the compound Tibetan schizonepeta composition with the effects of brightening skin and resisting allergy and relieving is provided, and is prepared from the following raw materials in parts by weight: 10-50 parts of Tibetan schizonepeta, 10-50 parts of corydalis tuber, 5-30 parts of tribulus fruit and 5-30 parts of myrobalan.
Further, the compound Tibetan schizonepeta composition with the effects of brightening skin and resisting allergy and relieving allergy is prepared from the following raw materials in parts by weight: 20-40 parts of Tibetan schizonepeta, 20-40 parts of corydalis tuber, 10-20 parts of tribulus fruit and 10-20 parts of myrobalan.
Further, the compound Tibetan schizonepeta composition with the effects of brightening skin and resisting allergy and relieving allergy is prepared from the following raw materials in parts by weight: 40 parts of Tibetan schizonepeta, 30 parts of corydalis tuber, 15 parts of tribulus fruit and 15 parts of myrobalan.
The herba Schizonepetae is whole plant of Nepeta angustifolia C.Y.Wu of Labiatae. Has effects in calming endogenous wind, relieving spasm, relieving swelling, and relieving pain, and can be used for treating epilepsy, wound swelling and pain, apoplexy, hemorrhagic cerebral infarction, wound, and pain, and contains nepetalactone, ursolic acid, oleanolic acid, and betulinic acid.
Rhizoma Corydalis is dried tuber of Corydalis yanhusuo W.T.Wang of Papaveraceae. Has warm nature and pungent and bitter taste, has effects of promoting blood circulation, activating qi-flowing, and relieving pain, and can be used for treating chest and hypochondrium pain, abdominal pain, thoracic obstruction, cardialgia, amenorrhea, dysmenorrhea, puerperal blood stasis, and traumatic injury with swelling and pain, and contains alkaloids such as corydaline and tetrahydropalmatine.
The fructus Tribuli fruit is dried mature fruit of Tribuli terrestris L. Has mild warm nature and pungent and bitter taste, has effects of suppressing hyperactive liver, resolving stagnation, promoting blood circulation, dispelling pathogenic wind, improving eyesight, and relieving itching, and can be used for treating headache, giddiness, chest and hypochondrium distending pain, breast closure, acute mastitis, conjunctival congestion, nebula, rubella, pruritus, and saponin components such as dioscin and lucidus stage.
The fructus Chebulae is dry mature fruit of Terminalia chebula Retz of Combretaceae or Terminalia villosa Retz. var. tomentella Kurt. Neutral in nature, bitter, sour and astringent in taste, has the effects of astringing intestines to stop diarrhea, astringing lung to relieve cough, lowering fire and relieving sore throat, is used for treating chronic diarrhea and dysentery, hematochezia and rectocele, lung deficiency and cough with dyspnea, chronic cough, pharyngalgia and hoarseness, and contains polyphenols such as myrobalic acid, chebulagic acid, gallic acid and the like.
The preparation method of the compound Tibetan schizonepeta composition with the effects of brightening skin and resisting allergy and relieving allergy comprises the following steps:
(1) pulverizing dried Tibetan herba Schizonepetae and rhizoma corydalis, sieving with 20 mesh sieve, mixing, adding 5-15 times of 30-80 vt% ethanol according to volume and mass ratio, reflux extracting for 1-3 times, each time for 1-3 hr, filtering, mixing filtrates, and concentrating under reduced pressure at 55-75 deg.C to obtain fluid extract; then adding water into the clear paste to disperse to 0.05-0.5g/mL, eluting by macroporous adsorption resin, collecting eluent, and drying under reduced pressure to obtain a composition A;
(2) pulverizing dried fructus Tribuli and fructus Chebulae, sieving with 20 mesh sieve, mixing, adding 10-30 times of water according to volume-to-mass ratio, reflux extracting for 1-3 times (1-3 hr each time), filtering, mixing filtrates, and concentrating to obtain fluid extract; then adding water into the clear paste to disperse to 0.05-0.5g/mL, eluting by macroporous adsorption resin, collecting eluent, and drying under reduced pressure to obtain a composition B;
(3) and (3) uniformly mixing the composition A obtained in the step (1) and the composition B obtained in the step (2) to obtain the compound Tibetan schizonepeta composition with the effects of brightening the skin, resisting allergy and relieving.
Further, in the steps (1) and (2), when the macroporous adsorption resin is eluted, the ratio of the diameter to the height of the resin is 1:3-8, the loading amount is 0.1-1g/mL calculated by the amount of the whole formula crude drug, the adsorption flow rate is 1-4BV/h, the resin is eluted by water for removing impurities by 2-10 times of the volume of the resin, the resin is eluted by 30-80% of ethanol for 2-8 times of the volume of the resin, and the elution flow rate is 2-8 BV/h.
Further, in the steps (1) and (2), the macroporous adsorption resin is AB-8, D101, HPD-100, HPD-300 or HPD-5000.
The compound Tibetan schizonepeta composition with the skin brightening, anti-allergy and relieving effects is applied to preparing skin care products with the skin brightening, anti-allergy and relieving effects.
Furthermore, the compound Tibetan herba schizonepetae composition with the effects of brightening skin and resisting allergy and relieving is added in an amount of 0.1-10%.
Furthermore, the compound Tibetan herba schizonepetae composition with the effects of brightening skin and resisting allergy and relieving is added in an amount of 1-8%.
Further, the skin care product is a face wash, a face cream, a soap, a cream, an emulsion, water, a mask or a gel.
In summary, the invention has the following advantages:
the traditional Tibetan medicine theory is taken as guidance, and the modern scientific and technological research results are combined, Tibetan schizonepeta, corydalis tuber, tribulus fruit and myrobalan are used in a matched mode, Tibetan schizonepeta and corydalis tuber are taken as monarch drugs, tribulus fruit is taken as a ministerial drug, myrobalan is taken as an adjuvant drug, the effects of brightening skin color, lightening darkness, resisting allergy, relieving and improving skin sensitivity are achieved, and the traditional Tibetan medicine composition can be used for daily skin care products.
Detailed Description
Example 1
A compound Tibetan herba schizonepetae composition with skin brightening, anti-allergy and relieving effects is prepared from the following raw materials in parts by weight: 40 parts of Tibetan schizonepeta, 30 parts of corydalis tuber, 15 parts of tribulus fruit and 15 parts of myrobalan.
The preparation method of the compound Tibetan schizonepeta composition with the effects of brightening skin and resisting allergy and relieving allergy comprises the following steps:
(1) pulverizing dried Tibetan herba Schizonepetae and rhizoma corydalis, sieving with 20 mesh sieve, mixing, adding 10 times of 50 vt% ethanol according to volume and mass ratio, reflux extracting for 2 times (2 hr each time), filtering, mixing filtrates, and concentrating under reduced pressure at 75 deg.C to obtain fluid extract; then adding water into the clear paste to disperse to 0.2g/mL, eluting by macroporous adsorption resin, collecting eluent, and drying under reduced pressure to obtain a composition A;
(2) pulverizing dried fructus Tribuli and fructus Chebulae, sieving with 20 mesh sieve, mixing, adding 15 times of water according to volume-mass ratio, reflux-extracting for 2 times (2 hr each time), filtering, mixing filtrates, and concentrating to obtain fluid extract; then adding water into the clear paste to disperse to 0.5g/mL, eluting by macroporous adsorption resin, collecting eluent, and drying under reduced pressure to obtain a composition B;
(3) and (3) uniformly mixing the composition A obtained in the step (1) and the composition B obtained in the step (2) to obtain the compound Tibetan schizonepeta composition with the effects of brightening the skin, resisting allergy and relieving.
In the steps (1) and (2), when the macroporous adsorption resin is eluted, HPD-100 type macroporous adsorption resin is adopted, the diameter-height ratio of the resin is 1:6, the loading amount is 0.5g/mL based on the amount of the whole formula crude drug, the adsorption flow rate is 2BV/h, the resin is eluted by water by 8 times of the volume of the resin to remove impurities, 50% ethanol is used for eluting by 5 times of the volume of the resin, and the elution flow rate is 2 BV/h.
Example 2
A compound Tibetan herba schizonepetae composition with skin brightening, anti-allergy and relieving effects is prepared from the following raw materials in parts by weight: 30 parts of Tibetan schizonepeta, 30 parts of corydalis tuber, 20 parts of tribulus fruit and 20 parts of myrobalan.
The preparation method of the compound Tibetan schizonepeta composition with the effects of brightening skin and resisting allergy and relieving allergy comprises the following steps:
(1) pulverizing dried Tibetan herba Schizonepetae and rhizoma corydalis, sieving with 20 mesh sieve, mixing, adding 5 times of 80 vt% ethanol according to volume and mass ratio, reflux extracting for 3 times, each time for 1 hr, filtering, mixing filtrates, and concentrating under reduced pressure at 65 deg.C to obtain fluid extract; then adding water into the clear paste to disperse to 0.5g/mL, eluting by macroporous adsorption resin, collecting eluent, and drying under reduced pressure to obtain a composition A;
(2) pulverizing dried fructus Tribuli and fructus Chebulae, sieving with 20 mesh sieve, mixing, adding 30 times of water according to volume-mass ratio, reflux extracting for 1 time, each time for 3 hr, filtering, mixing filtrates, and concentrating to obtain fluid extract; then adding water into the clear paste to disperse to 0.1g/mL, eluting by macroporous adsorption resin, collecting eluent, and drying under reduced pressure to obtain a composition B;
(3) and (3) uniformly mixing the composition A obtained in the step (1) and the composition B obtained in the step (2) to obtain the compound Tibetan schizonepeta composition with the effects of brightening the skin, resisting allergy and relieving.
In the steps (1) and (2), D-101 type macroporous adsorption resin is adopted when the macroporous adsorption resin is eluted, the ratio of the diameter to the height of the resin is 1:8, the loading amount is 1g/mL based on the amount of the whole formula crude drugs, the adsorption flow rate is 4BV/h, the resin is eluted by water by 5 times of the volume of the resin to remove impurities, the resin is eluted by 380 percent ethanol by 8 times of the volume of the resin, and the elution flow rate is 8 BV/h.
Example 3
A compound Tibetan herba schizonepetae composition with skin brightening, anti-allergy and relieving effects is prepared from the following raw materials in parts by weight: 40 parts of Tibetan schizonepeta, 40 parts of corydalis tuber, 10 parts of tribulus fruit and 10 parts of myrobalan.
The preparation method of the compound Tibetan schizonepeta composition with the effects of brightening skin and resisting allergy and relieving allergy comprises the following steps:
(1) pulverizing dried Tibetan herba Schizonepetae and rhizoma corydalis, sieving with 20 mesh sieve, mixing, adding 15 times of 30 vt% ethanol according to volume and mass ratio, reflux extracting for 1 time, each time for 3 hr, filtering, mixing filtrates, and concentrating under reduced pressure at 70 deg.C to obtain fluid extract; then adding water into the clear paste to disperse to 0.1g/mL, eluting by macroporous adsorption resin, collecting eluent, and drying under reduced pressure to obtain a composition A;
(2) pulverizing dried fructus Tribuli and fructus Chebulae, sieving with 20 mesh sieve, mixing, adding 10 times of water according to volume-mass ratio, reflux extracting for 3 times (3 hr each time), filtering, mixing filtrates, and concentrating to obtain fluid extract; then adding water into the clear paste to disperse to 0.5g/mL, eluting by macroporous adsorption resin, collecting eluent, and drying under reduced pressure to obtain a composition B;
(3) and (3) uniformly mixing the composition A obtained in the step (1) and the composition B obtained in the step (2) to obtain the compound Tibetan schizonepeta composition with the effects of brightening the skin, resisting allergy and relieving.
In the steps (1) and (2), when the macroporous adsorption resin is eluted, HPD-300 type macroporous adsorption resin is adopted, the diameter-height ratio of the resin is 1:3, the loading amount is 0.1g/mL based on the amount of the whole formula crude drug, the adsorption flow rate is 3BV/h, 6 times of the volume of the resin is eluted by water to remove impurities, 8 times of the volume of the resin is eluted by 30 percent ethanol, and the elution flow rate is 5 BV/h.
Example 4
A compound Tibetan herba schizonepetae composition with skin brightening, anti-allergy and relieving effects is prepared from the following raw materials in parts by weight: 30 parts of Tibetan schizonepeta, 40 parts of corydalis tuber, 20 parts of tribulus fruit and 10 parts of myrobalan.
The preparation method of the compound Tibetan schizonepeta composition with the effects of brightening skin and resisting allergy and relieving allergy comprises the following steps:
(1) pulverizing dried Tibetan herba Schizonepetae and rhizoma corydalis, sieving with 20 mesh sieve, mixing, adding 8 times of 60 vt% ethanol according to volume and mass ratio, reflux extracting for 2 times, each time for 2.5 hr, filtering, mixing filtrates, and concentrating under reduced pressure at 55 deg.C to obtain fluid extract; then adding water into the clear paste to disperse to 0.08g/mL, eluting by macroporous adsorption resin, collecting eluent, and drying under reduced pressure to obtain a composition A;
(2) pulverizing dried fructus Tribuli and fructus Chebulae, sieving with 20 mesh sieve, mixing, adding 25 times of water according to volume-to-mass ratio, reflux extracting for 2 times (2.5 hr each time), filtering, mixing filtrates, and concentrating to obtain fluid extract; then adding water into the clear paste to disperse to 0.08g/mL, eluting by macroporous adsorption resin, collecting eluent, and drying under reduced pressure to obtain a composition B;
(3) and (3) uniformly mixing the composition A obtained in the step (1) and the composition B obtained in the step (2) to obtain the compound Tibetan schizonepeta composition with the effects of brightening the skin, resisting allergy and relieving.
Wherein, in the steps (1) and (2), when the macroporous adsorption resin is eluted, HPD-5000 type macroporous adsorption resin is adopted, the diameter-height ratio of the resin is 1:7, the loading amount is 0.4g/mL calculated by the amount of the whole formula crude drugs, the adsorption flow rate is 2.5BV/h, the resin is eluted by 4 times of the volume of the resin with water to remove impurities, the resin is eluted by 8 times of the volume of the resin with 70 percent ethanol, and the elution flow rate is 7 BV/h.
Example 5
A compound Tibetan herba schizonepetae composition with skin brightening, anti-allergy and relieving effects is prepared from the following raw materials in parts by weight: 35 parts of Tibetan schizonepeta, 30 parts of corydalis tuber, 20 parts of tribulus fruit and 15 parts of myrobalan.
The preparation method of the compound Tibetan schizonepeta composition with the effects of brightening skin and resisting allergy and relieving allergy comprises the following steps:
(1) pulverizing dried Tibetan herba Schizonepetae and rhizoma corydalis, sieving with 20 mesh sieve, mixing, adding 12 times of 70 vt% ethanol according to volume and mass ratio, reflux extracting for 3 times, each time for 1.5 hr, filtering, mixing filtrates, and concentrating under reduced pressure at 60 deg.C to obtain fluid extract; then adding water into the clear paste to disperse to 0.4g/mL, eluting by macroporous adsorption resin, collecting eluent, and drying under reduced pressure to obtain a composition A;
(2) pulverizing dried fructus Tribuli and fructus Chebulae, sieving with 20 mesh sieve, mixing, adding 16 times of water according to volume-mass ratio, reflux extracting for 3 times (1.5 hr each time), filtering, mixing filtrates, and concentrating to obtain fluid extract; then adding water into the clear paste to disperse to 0.3g/mL, eluting by macroporous adsorption resin, collecting eluent, and drying under reduced pressure to obtain a composition B;
(3) and (3) uniformly mixing the composition A obtained in the step (1) and the composition B obtained in the step (2) to obtain the compound Tibetan schizonepeta composition with the effects of brightening the skin, resisting allergy and relieving.
In the steps (1) and (2), when the macroporous adsorption resin is eluted, AB-8 type macroporous adsorption resin is adopted, the ratio of the diameter to the height of the resin is 1:6, the loading amount is 0.8g/mL based on the amount of the whole formula crude drugs, the adsorption flow rate is 4BV/h, the resin is eluted by 7 times of the volume of water to remove impurities, the resin is eluted by 6 times of the volume of 40% ethanol, and the elution flow rate is 3 BV/h.
Comparative example 1
Comparative example 1 differs from example 1 in that only Tibetan catmint was included.
Comparative example 2
Comparative example 2 differs from example 1 in that only corydalis tuber is included.
Comparative example 3
Comparative example 3 differs from example 1 in that only tribulus fruit is contained.
Comparative example 4
Comparative example 4 differs from example 1 in that only myrobalan was included.
Comparative example 5
Comparative example 5 differs from example 1 in that only chenopodium ambrosioides and corydalis tuber are present.
Comparative example 6
Comparative example 6 differs from example 1 in that only the fruits of Tibet Schizonepeta and Tribulus terrestris are present.
Comparative example 7
Comparative example 7 differs from example 1 in that only Tibetan catnip and myrobalan were included.
Comparative example 8
Comparative example 8 differs from example 1 in that only corydalis tuber and tribulus fruit are contained.
Comparative example 9
Comparative example 9 differs from example 1 in that only corydalis tuber and myrobalan are contained.
Comparative example 10
Comparative example 10 differs from example 1 in that only tribulus fruit and myrobalan are present.
Comparative example 11
Comparative example 11 differs from example 1 in that myrobalan is not included.
Comparative example 12
Comparative example 12 differs from example 1 in that no corydalis tuber is included.
Comparative example 13
Comparative example 13 differs from example 1 in that catnip was not included.
Examples of the experiments
The compositions obtained in example 1 and comparative examples 1 to 6 were used to prepare corresponding skin creams according to the formulation shown in Table 1.
TABLE 1 skin cream formulation table
Figure BDA0003360970120000101
Heating the components of group A and B to 70 deg.C, dissolving completely, and mixing well. Slowly adding group A into group B under stirring, slowly cooling to 30 deg.C, adding group C, stirring, and cooling to room temperature to obtain skin cream. The skin cream of each proportion is prepared according to the same method.
1. Comparative experiment for skin color brightening effect
(1) A subject: selecting 140 subjects, female, 20-45 years old and indoor workers, wherein the exclusion conditions of the subjects meet the inclusion and exclusion standards of the cosmetic contact dermatitis diagnosis standard and treatment principle; no serious systemic disease, no immunodeficiency or autoimmune disease; an inactive allergic disease; no allergic history to skin care cosmetics; hormone medicine and immunosuppressant have not been used systemically in 1 month, and are not in gestation or lactation period; no ethical contraindication; skin color was tested between II-IV using healthy subjects according to Chardon's grouping. The subjects were randomly divided into 14 groups of 10 persons each, and skin color lightening effect comparison experiments were performed using the skin cream prepared by the composition of example 1 and the above comparative example.
(2) Testing indexes are as follows: skin whiteness (brightness) (L)*Value): l is*The value is white balance, and the color is more white when the value is larger, and is more black when the value is smaller.
Skin brightness (dark yellowness) (ITA ° value): ITA DEG value is the skin individual type angle, is equal to L*Value b and*value-dependent values characterizing skin brightness. The greater the ITA value, the brighter the skin, whereas the darker the skin.
The test method comprises the following steps: selecting the inner sides of the left arm crank and the right arm crank of the testee as tested areas (with the same area), randomly marking, setting blank areas (without using any sample), smearing experimental samples on the tested areas for 1 time in the morning and at night every day, and smearing the experimental samples according to personal use habits. The use period was 8 weeks. The subjects did not change the brand and habit of daily use of the cleaning product during the test. After 8 weeks of use of the test product, data were collected from the test site using a test instrument (Mexameter MX 18 skin melanin and heme tester, MPA9 multifunctional skin tester and colorimetric test probe, CK company, germany).
And (3) data analysis: if the data are normally distributed, the variation of the data before and after the use of the sample and the difference between the test area and the matrix area are analyzed by using the t-test. If the data are distributed in a non-normal way, corresponding statistical analysis is carried out by applying rank sum test, and a conclusion whether the data are effective or not is obtained according to a calculation result. The data calculation formulas are shown in formula (1) and formula (2).
Formula (1) Δ L*Value of L*Value (test area) -L*Value (blank), formula (2) Δ ITA ° value ═ ITA ° value (test area) -ITA ° (blank).
Skin whiteness (brightness) (L)*Values) the results are shown in table 2.
TABLE 2 skin whiteness (brightness) (L)*Value) measurement result
Figure BDA0003360970120000111
Figure BDA0003360970120000121
Note: in comparison with the example 1, the present inventors have conducted a comparison,*P<0.05，**P<0.01。
table 2 results show the L of the test area of subjects 8 weeks after using the product of example 1 of the present invention*The value is obviously increased, and compared with various comparative products, the value is obviously different (P)<0.05，P<0.01). Delta L of the product of example 1 of the invention in comparison with the blank zone of the same subject*The value is also significantly higher than that of the other comparative products (P)<0.01). The formula of the invention has obvious synergistic skin color brightening effect, and compared with a comparative product containing only one, two or three raw materials, the formula of the invention has obvious skin color brightening effect and definite synergistic effect.
The skin brightness (dark yellowness) (ITA DEG values) are determined in Table 3.
TABLE 3 skin Brightness (dark yellowness) (ITA DEG value) measurement results
Figure BDA0003360970120000122
Figure BDA0003360970120000131
Note: in comparison with the example 1, the present inventors have conducted a comparison,*P<0.05，**P<0.01。
the results in table 3 show that after 8 weeks using the product of example 1 according to the invention, the value of Δ ITA ° was also significantly higher for the product of example 1 according to the invention than for the other comparative products (P <0.01) in comparison with the Δ ITA ° values of the blank zone of the same subjects. The formula of the invention has obvious synergistic effect of increasing skin brightness, and compared with a comparative product containing only one, two or three raw materials, the skin color brightening effect is obvious, and the formula has definite synergistic effect.
2. Anti-allergic and antipruritic test
After 1 week of acclimatization of 160 mice, they were randomly divided into 16 groups: blank control group, histamine group, inventive example 1, comparative examples 1-13, 10 per group. In the experiment, 1% sodium sulfide is used for removing the hair of the mouse back, the area of the skin area for removing the hair is 2cm multiplied by 3cm, and the mouse is used for the experiment after 2 days. In the experiment, the back hairless area of each group of animals is locally coated with corresponding samples (0.2 ml/mouse) for 5 days continuously, and the neck and the back of each group of mice are injected with 50 mu L/mouse intracutaneously after 1h of the last administration except for a blank control group. After histamine administration, mice were observed for itching and the number of scratching was recorded within 30 min. The test data were statistically analyzed using SPSS 22.0 statistical software, and the statistics were expressed as mean. + -. standard deviation, and if the data were normally distributed, the variation of the data before and after the use of the sample and the difference between the test area and the matrix area were analyzed using the t-test method. If the data are distributed in a non-normal way, corresponding statistical analysis is carried out by applying rank sum test, and a conclusion whether the data are effective or not is obtained according to a calculation result. The results of the anti-allergic and antipruritic tests are shown in Table 4.
TABLE 4 anti-allergic antipruritic test results
Product group Number of scratching itch within 30min
Blank group 23.19±3.22##
Histamine group 153.31±20.23**
Example 1 33.81±12.68*##
Comparative example 1 94.27±28.30**##
Comparative example 2 73.43±19.56**##
Comparative example 3 65.70±21.77**##
Comparative example 4 148.92±32.68**
Comparative example 5 89.33±18.55**##
Comparative example 6 79.41±21.33**##
Comparative example 7 122.28±31.50**#
Comparative example 8 62.42±20.56**##
Comparative example 9 82.32±33.01**##
Comparative example 10 118.80±33.89**#
Comparative example 11 66.45±18.38**##
Comparative example 12 83.21±22.60**##
Comparative example 13 60.25±20.78**##
Note: in comparison with the blank set, the results,*P<0.05,**P<0.01. in comparison with the histamine group,#P<0.05，##P<0.01。
the results in table 4 show that, for itch caused by histamine, the product of the example of the invention has obvious anti-allergic itching relieving effect, and has significant difference (P <0.01) compared with histamine model group, although the itch scratching frequency is still higher than that of blank group, compared with other comparative example group, the itch scratching frequency of the product of the example 1 is obviously lower than that of other comparative example groups, and the itching relieving effect is obvious. The formula of the invention has obvious synergistic anti-allergy and relieving effects, and compared with a comparative product containing only one, two or three raw materials, the formula of the invention has obvious anti-allergy and itching relieving effects and has definite synergistic effect.
While the present invention has been described in detail with reference to the specific embodiments thereof, it should not be construed as limited by the scope of the present patent. Various modifications and changes may be made by those skilled in the art without inventive step within the scope of the appended claims.

Claims (10)

1. The compound Tibetan schizonepeta composition with the effects of brightening skin and resisting allergy and relieving is characterized by being prepared from the following raw materials in parts by weight: 10-50 parts of Tibetan schizonepeta, 10-50 parts of corydalis tuber, 5-30 parts of tribulus fruit and 5-30 parts of myrobalan.
2. The compound Tibetan catnip composition with skin lightening and anti-allergy relieving effects as claimed in claim 1, which is prepared from the following raw materials in parts by weight: 20-40 parts of Tibetan schizonepeta, 20-40 parts of corydalis tuber, 10-20 parts of tribulus fruit and 10-20 parts of myrobalan.
3. The compound Tibetan catnip composition with skin lightening and anti-allergy relieving effects as claimed in claim 1, which is prepared from the following raw materials in parts by weight: 40 parts of Tibetan schizonepeta, 30 parts of corydalis tuber, 15 parts of tribulus fruit and 15 parts of myrobalan.
4. A process for preparing a compound nepeta cataria composition for skin lightening and anti-allergy relief as claimed in any one of claims 1-3, comprising the steps of:
(1) pulverizing dried Tibetan herba Schizonepetae and rhizoma corydalis, sieving with 20 mesh sieve, mixing, adding 5-15 times of 30-80 vt% ethanol according to volume and mass ratio, reflux extracting for 1-3 times, each time for 1-3 hr, filtering, mixing filtrates, and concentrating under reduced pressure at 55-75 deg.C to obtain fluid extract; then adding water into the clear paste to disperse to 0.05-0.5g/mL, eluting by macroporous adsorption resin, collecting eluent, and drying under reduced pressure to obtain a composition A;
(2) pulverizing dried fructus Tribuli and fructus Chebulae, sieving with 20 mesh sieve, mixing, adding 10-30 times of water according to volume-to-mass ratio, reflux extracting for 1-3 times (1-3 hr each time), filtering, mixing filtrates, and concentrating to obtain fluid extract; then adding water into the clear paste to disperse to 0.05-0.5g/mL, eluting by macroporous adsorption resin, collecting eluent, and drying under reduced pressure to obtain a composition B;
(3) and (3) uniformly mixing the composition A obtained in the step (1) and the composition B obtained in the step (2) to obtain the compound Tibetan schizonepeta composition with the effects of brightening the skin, resisting allergy and relieving.
5. The method for preparing the compound Tibetan herba schizonepetae composition with the effects of brightening skin and resisting allergy and relieving pain as claimed in claim 4, wherein in the steps (1) and (2), when macroporous adsorption resin is eluted, the diameter-height ratio of the resin is 1:3-8, the loading amount is 0.1-1g/mL calculated by the amount of the whole formula crude drugs, the adsorption flow rate is 1-4BV/h, the resin volume which is 2-10 times of that of water elution is used for removing impurities, the resin volume which is 2-8 times of that of 30-80% ethanol elution is used for removing impurities, and the elution flow rate is 2-8 BV/h.
6. The method of claim 4, wherein the macroporous adsorbent resin of steps (1) and (2) is AB-8, D101, HPD-100, HPD-300, or HPD-5000.
7. Use of the compound Tibetan catnip composition having skin lightening and anti-allergy soothing effects as claimed in any one of claims 1 to 3 in the preparation of a skin lightening and anti-allergy soothing skin care product.
8. The use of claim 7, wherein the compound nepeta cataria composition is added in an amount of 0.1% to 10% for skin lightening and anti-allergy relief.
9. The use of claim 7, wherein the compound nepeta cataria composition is added in an amount of 1-8% for skin lightening and anti-allergy relief.
10. The use of claim 7, wherein the skin care product is a facial cleanser, facial cream, soap, cream, lotion, water, mask, or gel.
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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105055575A (en) * 2015-08-24 2015-11-18 北京太和逸肤科技有限公司 External application traditional Chinese medicine composition and preparation with skin whitening and qi and blood promoting effects and preparation method of external application traditional Chinese medicine composition
CN105250522A (en) * 2015-10-30 2016-01-20 北京工商大学 External use type traditional Chinese medicine composition with effect of preventing and treating mosquito bite, skin-protecting preparation and preparation method thereof
CN106420531A (en) * 2016-11-24 2017-02-22 青岛科技大学 Traditional Chinese medicine composite extract containing nepeta angustifolia and having skin whitening and moisturizing function and application
CN107802559A (en) * 2017-12-14 2018-03-16 广州市科能化妆品科研有限公司 Lightening compositions and its production and use
KR20200007501A (en) * 2018-07-13 2020-01-22 주식회사 코씨드바이오팜 Cosmetic composition for improvement of skin itching and moisturizing with the extract of Corydalis platycarpa
CN111544553A (en) * 2020-07-02 2020-08-18 青岛科技大学 Natural plant mosquito-repelling and itching-relieving composition containing volatile oil of Tibetan schizonepeta and application of natural plant mosquito-repelling and itching-relieving composition
CN111671713A (en) * 2020-06-28 2020-09-18 宗晨钟 Whitening and freckle removing cream and preparation method thereof
CN113521164A (en) * 2021-08-16 2021-10-22 西藏大学 Tibetan medicine composition with antibacterial effect and preparation method and application thereof

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105055575A (en) * 2015-08-24 2015-11-18 北京太和逸肤科技有限公司 External application traditional Chinese medicine composition and preparation with skin whitening and qi and blood promoting effects and preparation method of external application traditional Chinese medicine composition
CN105250522A (en) * 2015-10-30 2016-01-20 北京工商大学 External use type traditional Chinese medicine composition with effect of preventing and treating mosquito bite, skin-protecting preparation and preparation method thereof
CN106420531A (en) * 2016-11-24 2017-02-22 青岛科技大学 Traditional Chinese medicine composite extract containing nepeta angustifolia and having skin whitening and moisturizing function and application
CN107802559A (en) * 2017-12-14 2018-03-16 广州市科能化妆品科研有限公司 Lightening compositions and its production and use
KR20200007501A (en) * 2018-07-13 2020-01-22 주식회사 코씨드바이오팜 Cosmetic composition for improvement of skin itching and moisturizing with the extract of Corydalis platycarpa
CN111671713A (en) * 2020-06-28 2020-09-18 宗晨钟 Whitening and freckle removing cream and preparation method thereof
CN111544553A (en) * 2020-07-02 2020-08-18 青岛科技大学 Natural plant mosquito-repelling and itching-relieving composition containing volatile oil of Tibetan schizonepeta and application of natural plant mosquito-repelling and itching-relieving composition
CN113521164A (en) * 2021-08-16 2021-10-22 西藏大学 Tibetan medicine composition with antibacterial effect and preparation method and application thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
王丽丽: "植物延胡索化学成分的研究", no. 11, pages 340 *

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